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[application of pyrazinamidase activity for the identification of aeromonas species].70 aeromonas strains isolated from patients with acute diarrheal disease were classified into species by using the camp factor test and the pyrazinamidase activity. it is stressed the high percent of coincidence between both tests, which are easy to make at the clinical microbiology laboratories.19979685960
[comparative study of 4 methods for identifying species of the genus aeromonas].it is given an explanation of the results obtained with the following methods: aerokey ii, aerokey ii + abbot's scheme, api 20 ne, and the biolog system. the study was conducted with 38 strains of aeromonas isolated from children under 5 with acute diarrheal disease (add). the aerokey ii + abbot's scheme proved to be the best identification method.19979685968
polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria.polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ...199712501353
dissimilatory nitrate reduction in anaerobic sediments leading to river nitrite accumulation.recent studies on northern ireland rivers have shown that summer nitrite (no(inf2)(sup-)) concentrations greatly exceed the european union guideline of 3 (mu)g of n liter(sup-1) for rivers supporting salmonid fisheries. in fast-flowing aerobic small streams, no(inf2)(sup-) is thought to originate from nitrification, due to the retardation of nitrobacter strains by the presence of free ammonia. multiple regression analyses of no(inf2)(sup-) concentrations against water quality variables of the si ...199716535749
microbiological profile of raw nile water.with the increase of population on the nile banks, a remarkable increase in industrial, agricultural, human activities, and recreational activities have also occurred. the effluents of such activities are discharged directly into the nile or through some agricultural drains which finally discharge their wastes into the nile. thus, the microbiological monitoring is one of the main objectives to protect the river nile. this study was conducted to determine the microbiological profile of river nile ...199817217019
diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: aeromonas, plesiomonas, campylobacter.the reactiviteisofstrains ofplesionmona shigeloides, campylobater jejuni, campylobacter coli and aeromonas species with some lectins namely soyabean agglutinin (sba), ricinus communis agglutinin (rca), peanut agllutinin (pna), cancanavalin-a (con-a), lima bean agglutinin (lma) and abrin were investigated using the microtitre plate method. the results obtained show that all stains of a. hydrophila, a. caviae, a. sobria and p. shigelloides reacted very strongly with pna and sba. all strains of c. ...199817581001
the effects of pollution on fish health.potentially harmful substances-e.g. pesticides, heavy metals and hydrocarbons-are often released into the aquatic environment. when large quantities of pollutants are released there may be an immediate impact as measured by large-scale sudden mortalities of aquatic organisms, e.g. fish kills resulting from contamination of waterways with agricultural pesticides. lower levels of discharge may result in an accumulation of the pollutants in aquatic organisms. the end results, which may occur long a ...199821182713
bacteriocin activity and probiotic activity of aeromonas media.three strains of aeromonas media (161, a164 and a199) were shown to be active in-vitro producers of bacteriocin-like inhibitory substances (blis). for example, the producer strain, aer. media a199, displayed antagonistic activity against all strains tested of aer. caviae, aer. hydrophila, aer. salmonicida, aer. veronii var. sobria, listonella anguillarum, photobacterium damsella, eight species of vibrio and yersinia ruckeri. because of this wide-ranging activity against fish/shellfish pathogens, ...199821182714
specific probiotic characterization of weissella hellenica ds-12 isolated from flounder intestine.a total of 199 microorganisms were isolated from the intestinal contents of flounder (paralichthys olivaceus) in a fish farm in seoul, korea. among these strains, ds-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% nacl, 10% bile, and in broth at ph 3 for 90 min. this strain was gram-positive, and catalase-negative coccoid ...199812501410
lipase activity and gene cloning of acinetobacter calcoaceticus lp009.the production of lipase from acinetobacter calcoaceticus lp009, a bacterium isolated from raw milk, was found to be best induced by tween-80 at 1.0% concentration. it was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. a. calcoaceticus lp009 lipase exhibited maximum activity at ph 7.0 and 50 degrees c, and was relatively stable upon storage at ph 5.0 to 7.0 and at 4, 30, or 37 degrees c. the enzyme was found to be inactivated by e ...199812501281
bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria.on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ...199814666747
the development of random dna probes specific for aeromonas salmonicida.rapd-pcr has been used to produce dna probes for aeromonas salmonicida. dna hybridization studies showed that rapd-pcr fragments of the same size did not necessarily hybridize to each other and therefore these sequences were not always homologous. however, a single rapd-pcr fragment (designated 15e) was identified as being common to aer. salmonicida. subsequently, 15e was found to comprise five dna fragments of similar size which differed in their nucleotide sequences. all five fragments were ev ...199815244055
the acetylcholinesterase ichthyotoxin is a common component in the extracellular products of vibrionaceae strains.in previous work, it was reported that a strain of aeromonas hydrophila (b32) produces the most potent lethal toxin with neurotoxic activity described so far for fish. in the present study, the presence and distribution of this acetylcholinesterase toxin lethal for fish were determined in extracellular products (ecp) of 42 vibrionaceae strains using both immunological and colorimetric methods. this neurotoxin was shown to be present in the majority of the ecp from the aeromonas and vibrio strain ...199815244056
the maximum growth temperature and human pathogenicity of psychrotrophic aeromonads.determination of the maximum growth temperature of aeromonas strains isolated from clinical material and endowed with pronounced propensity for adhesion to intestinal cells has demonstrated that all strains still grow at 39 degrees c, with this holding true for the majority of strains also at 40 degrees c. since aeromonas isolated obtained from water evidence to a large extent a markedly lower maximum growth temperature, the 39 degrees c growth criterion is a characteristic of aeromonas strains ...19989686448
aeromonas hydrophila in burn patients.burn wound infection with aeromonas hydrophila appears to be very uncommon. this study reports on nine cases of a. hydrophila in burn patients treated over a 21 month period at the new somerset hospital burn unit. the average age of the patients was 31 years (range 24-60 years) and the average tbsa was 33% (range 16-51%). all patients had positive wound cultures for a. hydrophila, obtained on admission or shortly thereafter. one patient also had a positive blood culture. two patients with small ...19989688201
computer-assisted structural analysis of oligo- and polysaccharides: an extension of casper to multibranched structures.the casper program which is used for determination of the structure of oligo- and polysaccharides has been extended. it can now handle a reduced number of experimental signals from an nmr spectrum in the comparison to the simulated spectra of structures that it generates, an improvement which is of practical importance since all signals in nmr spectra cannot always be identified. furthermore, the program has been enhanced to simulate nmr spectra of multibranched oligo- and polysaccharides. the n ...19989691437
[bacteriological and virological studies on the cause of sporadic acute gastroenteritis in tama, tokyo (1991-1996)].between may 1991 and december 1996, bacteriological and virological investigations on the fecal samples from patient of infantile diarrhea (155 cases), or infectious gastroenteritis (1,409 cases) diagnosed with 9 medical institutions in tama of tokyo were carried out. of 1,564 samples, 722 (46.2%) were enteropathogen positive cases, and mixed infection was also observed in about 15% of the positive cases. among 13 different kinds of enteropathogens identified, the most prevalent one was pathogen ...19989695470
occurrence and significance of atypical aeromonas salmonicida in non-salmonid and salmonid fish species: a review.bacterial strains of aeromonas salmonicida included in the recognized subsp. acromogenes, subsp. masoucida, and subsp. smithia in addition to the large number of strains not included in any of the described subspecies are referred to as atypical a. salmonicida. the atypical strains form a very heterogeneous group with respect to biochemical characteristics, growth conditions, and production of extracellular proteasess. consequently, the present taxonomy of the species a. salmonicida is rather am ...19989696626
growth of atlantic salmon salmo salar after intraperitoneal administration of vaccines containing adjuvants.growth of atlantic salmon after intraperitoneal (i.p.) administration of adjuvanted vaccines was studied using groups of individually tagged fish held together in one tank or pen under commercial farming conditions. parallel experiments were initiated at 2 freshwater sites and 1 marine site. trivalent (vibriosis, cold water vibriosis and furunculosis) vaccines containing oil or beta-1, 3 glucan as adjuvants were used for immunisation of pre-smolts, whereas identical formulations containing furun ...19989696628
recurrent spontaneous bacterial empyema in cirrhosis: a case report.spontaneous bacterial empyema occurs in about 0.4% of cirrhotic patients, but recurrent attack has rarely been reported. herein we report a case of repeat episodes of spontaneous bacterial empyema. the patient presented with fever, shortness of breath and three episodes of spontaneous bacterial empyema (accompanied by septic shock in two episodes) within six months. chest roentgenography showed massive right-side pleural effusion. thoracentesis yielded pleural effusion containing a neutrophil co ...19989699395
aeromonas hydrophila sepsis presenting as meningitis and necrotizing fasciitis in a man with alcoholic liver cirrhosis.aeromonas hydrophila is rarely reported as a causative organism of meningitis in humans. a 39-year-old man with alcoholic liver cirrhosis was admitted with a 2-day history of fever, chills, and confusion. laboratory data revealed leukocytosis with granulocytosis, marked impairment of renal and liver function, and an elevated serum ammonia level. a. hydrophila was isolated from both blood and cerebrospinal fluid samples. skin and soft-tissue lesions, consisting of bullae and necrotizing fasciitis ...19989700248
the prevalence of vibrio spp. in drinking water and environmental samples in vellore south india.the prevalence of vibrio cholerae in drinking water, lakes and sewage outfalls during july and august 1996 in vellore, india was determined. drinking water samples were collected on single occasions from 12 sites in different geographic areas of the town where cholera had been reported. samples of water, plankton and sediment were collected from fixed sites at three lakes on three occasions separated by at least 3 days during the course of the study. samples from open sewers were taken from two ...19989747757
prolyl endopeptidase from sphingomonas capsulata: isolation and characterization of the enzyme and nucleotide sequence of the gene.prolyl endopeptidase (prolyl oligopeptidase, ec 3.4.21.26) was purified from sphingomonas capsulata ifo 12533, and its gene was cloned and expressed in escherichia coli. the recombinant enzyme was markedly inhibited by diisopropyl phosphofluoridate and hardly affected by sh reagents or metal chelators, similar to the native enzyme purified from s. capsulata. nucleotide sequencing analysis revealed an open reading frame of 2169 bp, coding for a protein of 723 amino acids with a predicted molecula ...19989750174
occurrence, diversity and pathogenicity of mesophilic aeromonas in estuarine waters of the italian coast of the adriatic sea.a total of 208 strains of aeromonas were isolated by monthly sampling from two estuaries (one provided with, and the other devoid of a waste-water treatment system) on the italian coast of the adriatic sea between september 1994 and august 1995. biotyping at the species level allowed the identification of 96 strains (46%) as aer. caviae, 46 (22%) as aer. sobria, 33 (16%) as aer. hydrophila and 25 (12%) as aer. veronii. eight strains (4%) were regarded as unnamed aeromonads. aeromonas caviae was ...19989750281
the occurrence of aeromonas species in drinking water supplies of an area of the dolomite mountains, italy.a study was made of the occurrence of aeromonas spp. in drinking water supplies in a mountain area in northeast italy (the dolomites). on account of its location, the water in question is exposed to a low level of pollution and systematic chemical disinfection is not necessary. out of 7395 water samples analysed over a 3 year period, 1623 (21 x 95%) were found to be positive for aeromonas, with levels ranging from 1 to 240 cfu 100 ml-1; 72 x 4% of the strains were identified as aer. hydrophila, ...19989750299
fishtank water as a source of a rare case of aeromonas hydrophila septicaemia. 19989754216
molecular properties and activity of a carboxyl-terminal truncated form of xylanase 3 from aeromonas caviae w-61.aeromonas caviae w-61 produces five species of xylanases, xylanases 1, 2, 3, 4, and 5 [nguyen, v.d. et al., biosci. biotechnol. biochem., 56, 1708-1712 (1993) and appl. environ. microbiol., 57, 445-449 (1991)]. while preserving a purified xylanase 3 preparation from a. caviae in solution at 4 degrees c, the xylanase 3 was found to be proteolyzed to give a truncated form with a smaller molecular mass than that of the intact one. it appears likely that the truncated form of xylanase 3 was produced ...19989757562
distribution and life strategies of two bacterial populations in a eutrophic lakemonoclonal antibodies and epifluorescence microscopy were used to determine the depth distribution of two indigenous bacterial populations in the stratified lake plusssee and characterize their life strategies. populations of comamonas acidovorans px54 showed a depth distribution with maximum abundances in the oxic epilimnion, whereas aeromonas hydrophila pu7718 showed a depth distribution with maximum abundances in the anoxic thermocline layer (metalimnion), i. e., in the water layer with the h ...19989758799
x-ray structure of the znii beta-lactamase from bacteroides fragilis in an orthorhombic crystal form.beta-lactamases are extracellular or periplasmic bacterial enzymes which confer resistance to beta-lactam antibiotics. on the basis of their catalytic mechanisms, they can be divided into two major groups: active-site serine enzymes (classes a, c and d) and the znii enzymes (class b). the first crystal structure of a class b enzyme, the metallo-beta-lactamase from bacillus cereus, has been solved at 2.5 a resolution [carfi, pares, duée, galleni, duez, frère & dideberg (1995). embo j. 14, 4914-49 ...19989761816
incidence & enteropathogenicity of aeromonas spp in children suffering from acute diarrhoea in chennai.a total of 200 stool samples from children below 10 yr suffering from diarrhoea were screened for enteric pathogens with special interest on aeromonas. aeromonas spp were isolated from 6.5 per cent of the patients, comprising 4 per cent a. hydrophila, 2 per cent a. sobria and 0.5 per cent a. caviae. among the 13 isolates obtained, 10 isolates produced enterotoxin in ligated rabbit ileal loops, and 11 produced cytotoxin in hep 2 cells. many of the aeromonas isolates exhibited resistance to common ...19989701892
motile aeromonads in the feces and carcasses of broiler chickens in turkey.motile aeromonas spp. were surveyed in chicken feces, carcasses, scalding water, and chilling water during slaughtering in a commercial processing plant in turkey. fecal and carcass samples of 351 chickens from 15 different flocks were examined by selective isolation techniques. all of the 15 flocks were positive for motile aeromonads. motile aeromonads were detected in 14.8%, 90.5%, and 100% of fecal, carcass, and chilling water samples, respectively, while scalding water samples were negative ...19989708264
inhibition of listeria monocytogenes and aeromonas hydrophila by plant extracts in refrigerated cooked beef.refrigerated ready-to-eat foods are becoming increasingly popular but are often vulnerable to contamination and subsequent growth by psychrotrophic foodborne pathogens. consequently, there is a need for additional methods to assure the safety of these foods. beef slices prepared from roasted whole sirloin tips were used in the study. nine plant extracts were evaluated for ability to inhibit the growth of two psychrotrophic pathogens (aeromonas hydrophila and listeria monocytogenes) in refrigerat ...19989708301
growth of inoculated psychrotrophic pathogens on refrigerated fillets of aquacultured rainbow trout and channel catfish.aquacultured rainbow trout (oncorhynchus mykiss) and channel catfish (ictalurus punctatus) fillets were inoculated with the psychrotrophic pathogens listeria monocytogenes and aeromonas hydrophila: cell populations were monitored during refrigerated storage at 2 to 4 degrees c. fillets of both species were placed individually in sterile plastic bags and inoculated with cell suspensions (10(4.7) cfu/100 g of fish) of either a. hydrophila or l monocytogenes or of both a. hydrophila and l. monocyto ...19989708302
survival of aeromonas hydrophila in fresh tomatoes (lycopersicum esculentum mill) stored at different temperatures and treated with chlorine.this study examines the survival of two aeromonas hydrophila strains (a. hydrophila atcc 7965 [strain a] and a. hydrophila isolated from food [strain b] on the surface and core tissue of fresh tomatoes stored at different temperatures and the efficacy of chlorine treatment on their survival. counts of a. hydrophila on the surface of tomatoes stored at 25 and 35 degrees c were significantly higher between days 1 and 4 for both strains as compared to results obtained at 6 degrees c. core tissue co ...19989709203
recovery of foodborne microorganisms from potentially lethal radiation damage.a two-stage recovery protocol was examined for microorganisms following gamma irradiation in phosphate buffer at 0 degrees c. in the first stage, survivors were recovered on basal yeast extract agar and held at various temperatures suboptimal for their growth for 20 h (resuscitation protocol). in the second stage the survivors were incubated for an additional 24 h, but in this case at their optimum temperature for growth. controls consisted of survivors which were not subjected to the resuscitat ...19989709232
bacterial populations associated with the dirty area of a south african poultry abattoir.bacterial populations associated with three sample types from the neck region of poultry carcasses in the dirty area of an abattoir were characterized. sample types before and after scalding were skin only, feathers only, and a skin and feather combination. the neck skin of carcasses after the defeathering processing stage was also sampled. bacterial populations associated with water from the scald tank, rubber fingers at the exit of the defeathering machine, and air in the dirty area were also ...19989709252
evolving concepts regarding the genus aeromonas: an expanding panorama of species, disease presentations, and unanswered questions.it has been almost 10 years since a major review on the association of aeromonas with human disease has been published. during that period the number of valid species in the genus has grown to 14, with a new family (aeromonadaceae) established to house this genus. despite this explosion in the number of new genomospecies, only five (aeromonas hydrophila, a. caviae, a. veronii, a. jandaei, and a. schubertii) are currently recognized as human pathogens. new syndromes attributed to this genus inclu ...19989709884
septic arthritis due to aeromonas veronii biotype sobria. 19989709899
metallo-beta-lactamases: a class apart.metallo-beta-lactamases have recently become more prominent among the beta-lactam-hydrolyzing enzymes. two major functional groups of enzymes have been identified, with little structural similarity among the groups. one group is a set of enzymes with broad substrate specificities capable of hydrolyzing most beta-lactams except monobactams. a second group is composed of the "true" carbapenemases, enzymes that exhibit poor hydrolysis of penicillins and cephalosporins. this latter group has been fo ...19989710671
identification of atypical aeromonas salmonicida: inter-laboratory evaluation and harmonization of methods.the atypical isolates of aeromonas salmonicida are becoming increasingly important as the frequency of isolation of bacteria belonging to this group continues to rise. the primary object of this study was to compare and evaluate the results obtained in various laboratories concerning the biochemical identification of atypical aer. salmonicida before and after standardization of media and methods. five laboratories examined 25 isolates of aer. salmonicida from diverse fish species and geographica ...19989717284
antibacterial properties of extracts from selected planktonic freshwater cyanobacteria--a comparative study of bacterial bioassays.aqueous and methanol extracts from five selected cyanobacteria were examined for antibacterial properties in six different bacterial bioassays. all five cyanobacteria revealed antibacterial properties. methanol extracts made from tychonema bourrellyi, aphanizomenon flos-aquae and cylindrospermopsis raciborskii showed the most pronounced inhibitory effects, aqueous extracts made from microcystis aeruginosa and t. bourrellyi possessed evident antibacterial properties. the bacterial bioassays were ...19989717298
rrna-targeted fluorescent in situ hybridization analysis of bacterial community structure in river water.an improved in situ hybridization technique, hnpp-fish, using 2-hydroxy-3-naphthoic acid 2'-phenylanilide phosphate (hnpp) and fast red tr was applied to analyse the community structure of planktonic bacteria in river water. oligonucleotide probes specific for the domain bacteria (eub338) and five bacterial groups [flavobacterium-cytophaga; burkholderia-pseudomonas (rrna iii)-authentic alcaligenes; vibrio-aeromonas; pseudomonas (rrna i): the genus acinetobacter] were used to investigate the bact ...19989720029
physical map of the chromosome of aeromonas salmonicida and genomic comparisons between aeromonas strains.i-ceul and pmel physical maps of the aeromonas salmonicida a449 chromosome were constructed using pfge. the circular chromosome of a. salmonicida a449 was estimated to be 4658 +/- 30 kb. the approximate location of several genes, including those encoding proteins implicated in virulence, were identified. the map showed that the known virulence-factor-encoding genes were not clustered. the i-ceul genomic digestion fingerprints of several typical and atypical strains of a. salmonicida were compare ...19989720035
incidence of mesophilic aeromonas within a public drinking water supply in north-east scotland.the motile mesophilic aeromonas are ubiquitous to a wide variety of aquatic environments including drinking water distribution systems. concern over the presence of mesophilic aeromonas in public drinking water supplies has been expressed in recent years as it has been regarded as a pathogenic organism of importance in gastroenteritis. a major drinking water distribution system in north-east scotland was monitored over a 12 month period to determine the prevalence of mesophilic aeromonas. these ...19989721643
identification of aeromonas strains of different origin to the genomic species level.a total of 71 aeromonas strains was identified with established genomic species by dna-dna hybridization. the strains were isolated from diarrhoeal stools, dead and live fish, drinking, lake, river and sea water, municipal sewage and aluminium rolling emulsion. the strains were allocated to seven hybridization groups (hgs) but the majority belonged to hg 4 (42%), hg 8/10 (30%) and hg 3 (18%). all strains were examined by 136 phenotypic tests. useful phenotypic characters for separation of aeromo ...19989721647
bacterial isolates from california sea lions (zalophus californianus), harbor seals (phoca vitulina), and northern elephant seals (mirounga angustirostris) admitted to a rehabilitation center along the central california coast, 1994-1995.in 2 yr of bacteriologic culturing of 297 california sea lions (zalophus californianus), 154 harbor seals (phoca vitulina), and 89 northern elephant seals (mirounga angustirostris) stranded alive along the california coast, the most frequent isolates from inflammatory lesions in live animals were escherichia coli, streptococcus viridans, listeria ivanovii, beta-hemolytic streptococcus spp., and enterococcus spp. this is the first report of l. ivanovii isolation from a marine mammal. the common i ...19989732032
characterization of atypical aeromonas salmonicida by different methods.fifty two isolates of atypical aeromonas salmonicida, recovered from a wide range of hosts and geographical locations, were heterogeneous in terms of molecular and phenotypic characteristics, and represented taxa which could not be accommodated by the current classification of four subspecies. generally, there was incongruence between the molecular (pcr, rapd and ribotyping) and phenotypic methods in terms of cluster membership. by pcr, 6 groups were described of which group 1 encompassed 12 iso ...19989741110
antibacterial effects of n-sulfonated and n-sulfobenzoyl chitosan and application to oyster preservation.the antibacterial effects of sulfonated and sulfobenzoyl chitosans were evaluated and compared with that of 69% deacetylated chitosan (dd69 chitosan). minimal inhibitory concentrations of sulfonated chitosan (sc1, 0.63% sulfur content) against shigella dysenteriae, aeromonas hydrophila, salmonella typhimurium, and bacillus cereus were found to be lower than those of dd69 chitosan. a high sulfur content in sulfonated chitosan adversely influenced its antibacterial effect. sulfobenzoyl chitosan (s ...19989766062
mesophilic aeromonas strains from different serogroups: the influence of growth temperature and osmolarity on lipopolysaccharide and virulence.growth of mesophilic aeromonas sp. strains from serogroups o:13, o:33 and o:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (lps) and virulence of the strains tested, as we had previously reported for strains from serogroup o:34. the effect of osmolarity could be observed when the cells grew at 37 degrees c but not at 20 degrees c. purified lps from cells cultivated at 20 degrees c (high or low osmolarity) or at 37 degrees c at high osmolarity was smooth ...19989766240
an exeab complex in the type ii secretion pathway of aeromonas hydrophila: effect of atp-binding cassette mutations on complex formation and function.the energy-dependent secretion of aerolysin by aeromonas hydrophila requires the exea and exeb proteins. an 85 kda complex containing the two proteins was identified in wild-type cells but not in cells producing either protein alone. radiolabelling followed by cross-linking, immunoprecipitation and then reduction of the cross-links confirmed the presence of the two proteins in the same complex. the complex could also be extracted intact from cell membranes with non-ionic detergents. a g229d subs ...19989767591
pharmacokinetics of oxytetracycline in the red pacu (colossoma brachypomum) following different routes of administration.oxytetracycline (otc) pharmacokinetics were studied in the red pacu (colossoma brachypomum) following intravenous (i.v.) and intramuscular (i.m.) administration at a dose of 5 mg/kg body weight. otc plasma concentrations were determined by high-performance-liquid-chromatography (hplc). a non-compartmental model was used to describe plasma drug disposition after otc administration. following i.m. administration, the elimination half-life (t1/2) was 62.65 +/- 1.25 h and the bioavailability was 49. ...19989811436
population dynamics of aeromonas spp. in an urban river watershed.density of aeromonas spp. at one site in the buffalo river and at four sites on its upstream tributaries was followed from june 1992-june 1993. membrane filtration counts of aeromonas during the summer ranged between 18 and 4000 ml-1, which were one to two logs higher than faecal coliform and faecal streptococci densities. aeromonas spp. in the buffalo river, and faecal coliforms, faecal streptococci, and the heterotrophic plate count throughout the watershed, increased by approximately one log ...19989812384
isolation of aeromonas salmonicida in association with purple-pigmented bacteria in sediment from a scottish loch.aeromonas salmonicida was recovered in close association with an unidentified purple-pigmented organism, which was isolated from sediment in a scottish loch during november (1997) and february (1998). however, there has not been any evidence of a. salmonicida infections, specifically furunculosis, associated with the fish in this loch.19989871353
construction of a physical and preliminary genetic map of aeromonas hydrophila jmp636.a physical and preliminary genetic map of the aeromonas hydrophila jmp636 chromosome has been constructed. the topology of the genome was predicted to be circular as chromosomal dna did not migrate from the origin during pfge unless linearized by s1 nuclease. cleavage of the chromosome with paci and pmei produced 23 and 14 fragments, respectively, and enabled calculation of the genome size at 4.5 mb. digestion of the chromosome with i-ceui produced 10 fragments, indicating that 10 rrl (23s) gene ...19989846744
mobile drug resistance genes among fish bacteria. 19989850686
transcriptional regulation and organization of the dcua and dcub genes, encoding homologous anaerobic c4-dicarboxylate transporters in escherichia coli.the dcua and dcub genes of escherichia coli encode homologous proteins that appear to function as independent and mutually redundant c4-dicarboxylate transporters during anaerobiosis. the dcua gene is 117 bp downstream of, and has the same polarity as, the aspartase gene (aspa), while dcub is 77 bp upstream of, and has the same polarity as, the anaerobic fumarase gene (fumb). to learn more about the respective roles of the dcu genes, the environmental and regulatory factors influencing their exp ...19989852003
[bacteriological quality of spring and well water in yaounde (cameroon)].the drinking water supply in yaounde, cameroon, often fails to meet demand and this leads many people to seek water from springs and wells the microbiological quality of which is unknown. we carried out a microbiological survey of five spring water points and ten wells. the fifteen study sites were located in various residential districts and were selected according to geographical location and number of users. microorganisms were isolated on specific culture media using the membrane filter tech ...19989854008
optimisation of the protocol for detection of aeromonas species in ready-to-eat salads, and its use to speciate isolates and establish their prevalence.aeromonas spp. are detected in more than 500 cases of gastrointestinal infection each year in england and wales. this study aimed to identify their prevalence in ready-to-eat salads, which are a potential source of aeromonas infection. the protocol for isolation of mesophilic aeromonas spp. from salads was optimised. using the improved method, aeromonas spp were isolated from 19 of 25 samples (25 g) of ready-to-eat salad products. aeromonas organisms were counted, isolates were identified to spe ...19989854886
the risk to public health of aeromonas in ready-to-eat salad products.mesophilic aeromonas spp. are isolated regularly from cases of gastrointestinal infection and have markers indicative of enteropathogenicity. is aeromonas, which is present in a large proportion of ready-to-eat salads, actually a gastrointestinal pathogen? isolates of mesophilic aeromonas from salads were characterised in terms of their ability to grow at refrigeration temperatures over the given shelf life and by the presence of markers of potential virulence. the major phenospecies present in ...19989854887
purification and characterization of enterotoxin produced by aeromonas sobria.we purified the toxin of aeromonas sobria capable of inducing a positive response in the mouse intestinal loop assay. the purified toxin showed a positive response not only in the loop assay but also in a hemolytic assay. subsequently, we cloned the toxin gene and demonstrated that the product of this gene possessed both hemolytic and enterotoxic activities. these results showed that the enterotoxin of a. sobria possesses hemolytic activity. nucleotide sequence determination of the toxin gene an ...19989858466
the acute phase response in the sudan plated lizard, gerrhosaurus major.the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ...19989773512
inducible beta-lactam resistance in aeromonas hydrophila: therapeutic challenge for antimicrobial therapy.despite the abundant amount of knowledge about inducible chromosomally mediated beta-lactamases among aeromonas species, extended-spectrum beta-lactam-resistant a. hydrophila strains selected in clinical practice were rarely reported. in the present study, two strains of a. hydrophila, a136 and a139, with markedly different susceptibilities to extended-spectrum cephalosporins were isolated from blood and the tip segment of an arterial catheter of a burn patient. another strain (a136m) was select ...19989774563
haemorrhagic septicaemia by aeromonas salmonicida subsp. salmonicida in a black-tip reef shark (carcharhinus melanopterus).one black-tip reef shark (carcharhinus melanopterus) was found dead without previous signs of disease. major lesions consisted in cutaneous erythema, mainly at the base of the fins, focal to diffuse inflammatory lesions in gills and intestinal wall, and discrete haemorrhages in the same organs, liver and kidneys. microcolonies of gram-negative rods were observed in the lamina propia of gills, underneath the intestinal mucosa and randomly distributed in the renal and hepatic parenchymas. also, em ...19989780832
bacteriophage pm2 nomenclature revision. 19989787669
plasma proteins of rainbow trout (oncorhynchus mykiss) isolated by binding to lipopolysaccharide from aeromonas salmonicida.in an attempt to find plasma proteins that might be involved in the constitutive resistance of rainbow trout to furunculosis, a disease caused by aeromonas salmonicida (as), we purified serum and plasma proteins based on their calcium- and carbohydrate-dependent affinity for a. salmonicida lipopolysaccharide (lps) coupled to an epoxy-activated synthetic matrix (toyopearl af epoxy 650m). a multimeric family of high molecular weight (96 to 200-kda) lps-binding proteins exhibiting both calcium and ...19989787816
experimental infections of rana esculenta with aeromonas hydrophila: a molecular mechanism for the control of the normal flora.frogs can be useful models for studying the mechanisms that may regulate their natural microbial flora. their skin glands produce a secretion containing 20-30 different peptides, some antimicrobial some neurotrophic. as they often live in soil or silt that is rich in microbes, they can be expected to be able to prevent or eliminate infections in very short periods of time. the bacterium aeromonas hydrophila is widely distributed in nature and is considered as part of the natural flora of frogs a ...19989790305
endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of vibrio cholerae.the chia gene of vibrio cholerae encodes a polypeptide which degrades chitin, a homopolymer of n-acetylglucosamine (glcnac) found in cell walls of fungi and in the integuments of insects and crustaceans. chia has a coding capacity corresponding to a polypeptide of 846 amino acids having a predicted molecular mass of 88.7 kda. a 52-bp region with promoter activity was found immediately upstream of the chia open reading frame. insertional inactivation of the chromosomal copy of the gene confirmed ...19989791107
secretion and properties of the large and small lobes of the channel-forming toxin aerolysin.aerolysin is a dimeric protein secreted by aeromonas spp. that binds to glycosylphosphatidylinositol-anchored receptors on target cells and becomes insertion competent by oligomerizing. the protein comprises two lobes joined by a short arm. the large lobe is thought to be responsible for channel formation, whereas the small lobe is believed to stabilize the dimer, and it may also contain the receptor binding site. we cloned and expressed the dna for both lobes of the toxin separately and togethe ...19989791179
occurrence of heterotrophic bacteria and fungi in an aviation fuel handling system and its relationship with fuel fouling.clean, dry and contaminant-free fuel is necessary for safe and economical aircraft operation. microbial growth in aviation fuel handling systems can alter the quality of the product. this paper reports the occurrence of heterotrophic bacteria and fungi in a handling system of jet a-1 aviation turbine fuel. a total of 350 samples were collected during 1990-1996. the aerobic microorganisms in fuel samples were mainly fungi, 85% of samples containing < or = 100 cfu/l (range 0 (< 1 cfu/l) to 2000 cf ...19989793139
molecular characterization of plasmid-mediated oxytetracycline resistance in aeromonas salmonicida.using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (ot)-resistant isolates of aeromonas salmonicida transferred the ot resistance phenotype to escherichia coli. the ot resistance phenotype was encoded by high-molecular-weight r-plasmids that were capable of transferring ot resistance to both environmental and clinical isolates of aeromonas spp. the molecular basis for antibiotic resistance in ot-resistant isolates of a. salmonicida was determined. the ot resistance determinant ...19989797265
adhesion of aeromonas hydrophila to water distribution system pipes after different contact times.scanning electron microscopy observation was used to investigate the ability of aeromonas hydrophila to attach to various water distribution pipe surfaces, such as stainless steel, copper, and polybutylene, after different contact times at ambient and storage temperatures. surface energy value of each surface was estimated by contact angle measurements using water, alpha-bromonaphthalene, and dimethyl sulfoxide. our results indicated that aeromonas cells could easily attach to all surface types ...19989798149
susceptibility of antibiotic-resistant and antibiotic-sensitive foodborne pathogens to acid anionic sanitizers.acid anionic sanitizers for treatment of fruits and vegetables were prepared using ingredients generally recognized as safe by the u.s. food and drug administration or anionic surfactants and organic acid food additives. they met the regulatory definition as sanitizers by showing bactericidal efficacy of 99.999% in 30 s against staphylococcus aureus atcc 6538 and escherichia coli atcc 11229. these sanitizers showed a broad spectrum of microbicidal activity against both gram-positive and gram-neg ...19989798163
sensitivity pattern and beta-lactamase production in clinical isolates of aeromonas strains.of the 43 aeromonas spp. isolated from various clinical samples 94% isolates were beta-lactamase producers. the isolates were tested for sensitivity by disc diffusion method which is commonly used in pakistan. mic was determined by using epsilometer test (e-test) method. more than 80% isolates were sensitive to cephalosporins and quinolones. however, resistance to commonly used antibiotics was very high, 94% isolates were resistant to ampicillin which corresponds to the betalactamase production. ...19989813984
[sanitary and microbiological assessment of marketable fish of the volga-caspian sea region].opportunistic microbes, such as aeromonads, enterobacteria, and pseudomonads, which are responsible for spoiling fish during its storage and processing and capable to initiate pathological processes of fishes are common in the volga-caspian sea region. investigations demonstrated that it is necessary to develop new procedures for processing fish products, to carry out studies on regulation of microbiological criteria for assessing fish quality. this makes it necessary to make a bacteriological c ...19989816781
[factors of persistence and (or) pathogenicity in vibrios and aeromonads belonging to different ecotopes].factors of persistence and/or pathogenicity in vibrio parahaemolyticus and aeromonas hydrophila (hemolytic, lipase, lecithin, dnaase, rnaase, antilysozyme, "anti-interferon", anticomplementary activities and capacity for absorbing congo red) were studied. the study revealed the interspecific and subpopulation (hospital and extraorganismal parts of the population) differences in the activity of the manifestation of these factors. strong dependence of the whole complex of persistence and pathogeni ...19989825495
[database on nucleotide sequences used as primers of microorganisms].the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ...19989825498
brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes.brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. in this study, we have examined the intracellular pathway of the virulent b. abortus strain 2308 (s2308) and the attenuated strain 19 (s19) in hela cells. at 10 min after inoculation, both bacterial strains are transiently detected in phagosomes characterized by the presence of early endosomal markers such as the early endosomal antigen 1. at approximately 1 h postinoculation, bacteria are locate ...19989826346
the pore-forming toxin proaerolysin is activated by furin.aerolysin is secreted as an inactive dimeric precursor by the bacterium aeromonas hydrophila. proteolytic cleavage within a mobile loop near the c terminus of the protoxin is required for oligomerization and channel formation. this loop contains the sequence kvrrar432, which should be recognized by mammalian proprotein convertases such as furin, pace4, and pc5/6a. here we show that these three proteases cleave proaerolysin after arg-432 in vitro, yielding active toxin. we also investigated the p ...19989830006
detection and isolation of novel rhizopine-catabolizing bacteria from the environmentmicrobial rhizopine-catabolizing (moc) activity was detected in serial dilutions of soil and rhizosphere washes. the activity observed generally ranged between 10(6) and 10(7) catabolic units per g, and the numbers of nonspecific culture-forming units were found to be approximately 10 times higher. a diverse set of 37 isolates was obtained by enrichment on scyllo-inosamine-containing media. however, none of the bacteria that were isolated were found to contain dna sequences homologous to the kno ...19989835587
necrotizing soft tissue infection in heart transplantation recipients: two case reports. 19989838477
effect of "acidic oxidative potential water" on microbial contamination harvesting porcine pancreas for islet xenotransplantation. 19989838512
inhibition of streptomyces griseus aminopeptidase and effects of calcium ions on catalysis and binding--comparisons with the homologous enzyme aeromonas proteolytica aminopeptidase.streptomyces griseus aminopeptidase is a zinc metalloenzyme containing 2 mol zinc/mol protein, similar to the homologous enzyme aeromonas proteolytica aminopeptidase. in addition, a unique ca2+-binding site has been identified in the streptomyces enzyme, which is absent in the aeromonas enzyme. binding of ca2+ enhances stability of the streptomyces enzyme and modulates its activity and affinity towards substrates and inhibitors in a structure-dependent manner. among the three hydrophobic 4-nitro ...19989874195
a collagen-binding protein produced by aeromonas hydrophila.the gastrointestinal pathogen aeromonas hydrophila strain a186 produces a collagen-binding protein (cnbp) which is found extracellularly and loosely associated with the cell surface. the cell-associated cnbp was purified by sequential ammonium sulphate precipitation, size-exclusion chromatography and ion-exchange chromatography, or by sequential ammonium sulphate precipitation and affinity chromatography with collagen-sepharose. the purified cnbp was homogeneous in sds-page, and had a mol. wt of ...19989879942
a cluster of cases of infections due to aeromonas hydrophila revealed by combined rapd and eric-pcr.two polymerase chain reaction (pcr)-based methods were used for epidemiological typing of aeromonas hydrophila. random amplification of polymorphic dna (rapd) and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) were applied to an outbreak involving seven patients. the epidemiological situation appeared complex; with the exception of two clinical isolates, all gave unique patterns with both techniques. these methods demonstrated nosocomial transmission in one unit and permitted the ...19989879968
characterisation of a type iv pilus produced by aeromonas caviae.a pilus produced by a clinical isolate of aeromonas caviae (strain ca195) was purified and partially characterised. the mr of the pilin was estimated to be 23 kda by sds-page. its n-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type iv pili purified from other aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. bundle-forming pili, often with a polar location, were seen on the surface of strain ca19 ...19989879972
virulence factors of aeromonads--an emerging food borne pathogen problem.aeromonas spp. possess a number of virulence properties which are considered responsible for intestinal and extra-intestinal infections in human beings and also for a wide variety of infections in animals. the paper discusses current status of colonization and toxic factors of aeromonas spp., especially in relation to food and biochemical markers. future research needs are also identified.19989914671
aeromonas hydrophila associated with a severe outbreak of infection in farmed rabbits.an outbreak of infection with a high rate of mortality has been detected in an industrial rabbitry in spain. aeromonas hydrophila was isolated in pure culture from liver, lung, heart and spleen of ill and moribund animals. all the isolates belonged to o:11 serogroup, autogglutinated after boiling, were resistant to the bactericidal action of the fresh rabbit serum and did not agglutinate in acriflavine. they were producers of hemolysins and proteases but were not enterotoxigenic.19989916296
[meningitis by aeromonas sobria following cranio-encephalic traumatism]. 19989919002
an attempt to use selected strains of bacteria adapted to high concentrations of petroleum oil to increase the effective removal of petroleum products in excess activated sludge in laboratory conditions.forty two strains of bacteria were isolated from excess activated sludge from petroleum wastewater treatment plant. the strains were identified and classified to the following groups: enterobacteriaceae (7 strains), anitratum (3 strains), pseudomonas (13 strains), micrococcus (12 strains), comamonas (2 strains), xanthomonas (2 strains), achromobacter (1 strain) and vibrio-aeromonas (1 strain). one of the isolates was found to be a yeast strain. following preliminary selection ten strains, showin ...19989990713
abdominal ascites in electric eels (electrophorus electricus) associated with hepatic hemosiderosis and elevated water ph.six electric eels (electrophorus electricus) from various centers that house aquatic organisms presented clinically with abdominal distension following prolonged exposure to elevated environmental ph. postmortem examination revealed marked ascites. culture of the abdominal fluid from three of the eels yielded either aeromonas hydrophila or citrobacter freundii, which were most likely secondary invaders. histopathology showed marked iron accumulation in both hepatocytes and hepatic macrophage agg ...199810065849
resistance to antibiotic and heavy metals of motile aeromonads from chilean freshwater.in this work the resistance of 172 motile aeromonas isolates recovered from raw drinking water supplies (56), irrigation waters (60) and runoff waters receiving sewage (56), to some antibiotics and heavy metals was investigated by agar diffusion and agar dilution methods. a high proportion of isolates from all water sources showed resistance to carbenicillin, erythromycin, streptomycin, cephradine and cadmium, and susceptibility to chloramphenicol, kanamycin, gentamicin, tetracycline, nalidixic ...19989926432
ulcerative dermatitis caused by aeromonas salmonicida spp. salmonicida in farmed atlantic salmon in british columbia. 199810051960
the production of a novel carbapenem-hydrolysing beta-lactamase in aeromonas veronii biovar sobria, and its association with imipenem resistance. 199810052912
distributional patterns of bacterial diarrhoeagenic agents and antibiograms of isolates from diarrhoeaic and non-diarrhoeaic patients in urban and rural areas of nigeria.to determine the prevalence of bacteria that could cause diarrhoea in stool specimens of individuals with and without diarrhoea in both urban and rural areas of nigeria. to ascertain the antibiotic susceptibilities of the bacterial diarrhoeagenic agents isolated. to document the predominant signs and symptoms associated with the various bacterial agents of diarrhoea.199810101427
identification of bacteria in water for pharmaceutical use.different systems for the obtention of water used in biopharmaceutical industry were characterized from the bacteriological point of view. determination of aerobic mesophilic microorganisms was performed; as well as the isolation of contaminant microorganisms for what the techniques of membrane filtration was used. for the identification of the more representative species there were made conventional biochemical tests and quick systems: api. the results show that water serving as tap water for p ...199810932743
expression and export of pseudomonas putida ntu-8 creatinase by escherichia coli using the chitinase signal sequence of aeromonas hydrophila.the gene for the creatinase from pseudomonas putida ntu-8 was sequenced and revealed an open reading frame (orf) of 1209 base pairs encoding a polypeptide of 403 amino acids with a calculated molecular weight (m(r)) of 45,691. the deduced amino acid sequence is very similar to that of the creatinase of pseudomonas putida and flavobacterium sp. an overproduction system for the chitinase signal peptide--creatinase hybrid gene was constructed by using the pqe-51 expression vector in e. coli jm109. ...199810230521
leech-borne serratia marcescens infection following complex hand injury.leeches are commonly used in the postoperative course of plastic surgical operations where there is venous congestion in a pedicled or free flap. they provide a temporary relief to venous engorgement whilst venous drainage is re-established. it is known that leeches can carry aeromonas hydrophila infection, and a second or third generation cephalosporin antibiotic has traditionally been given as prophylaxis against infection. we report a new observation that leeches can carry serratia marcescens ...199810209471
prevalence, in-vitro secretory activity, and cytotoxicity of aeromonas species associated with childhood gastroenteritis in chennai (madras), india.an investigation on the prevalence of aeromonas in gastrointestinal illnesses of pediatric inpatients 1 month to 3 years of age was conducted from february 1997 through january 1998 in madras. sixteen aeromonas spp. were isolated from 11 male and five female children among the 341 pediatric inpatients suffering from acute diarrhoea. a. caviae, which was isolated from nine cases, was found to be the most predominant isolate, followed by a. veronii biovar sobria, isolated from six cases, and a. hy ...199810211427
escherichia coli in patients with acute gastroenteritis in lagos, nigeria.over a period of 12 months, a total of 852 stool samples from patients (both children and adults) with acute diarrhoeal diseases attending some public and government recognised health institutions in lagos metropolis were screened for diarrhoeagenic bacterial agents. one hundred and eighty two bacterial isolates were found out of which 83 (45.6%) were escherichia coli, 38 (20.9%) shigella spp, 31 (17%) salmonella spp, 16 (8.8%) klebsiella spp, eight (4.4%) aeromonas spp, while there were only si ...199810493052
coordination chemistry of the amonabactins, bis(catecholate) siderophores from aeromonas hydrophila(1).the amonabactins are a series of four bis(catecholate) siderophores isolated from the pathogenic organism, aeromonas hydrophila. as tetradentate ligands, they cannot singly satisfy the octahedral coordination sphere of iron. the solution coordination chemistry of the amonabactins has been elucidated using potentiometric and spectrophotometric titrations, circular dichroism, and mass spectroscopy. they form 2:3 metal:ligand complexes at high ph and excess ligand. their complexation behavior is es ...199811670604
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