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necrotizing soft tissue infection in heart transplantation recipients: two case reports. 19989838477
effect of "acidic oxidative potential water" on microbial contamination harvesting porcine pancreas for islet xenotransplantation. 19989838512
polyphosphate-accumulating and denitrifying bacteria isolated from anaerobic-anoxic and anaerobic-aerobic sequencing batch reactorsin this study, phosphate-accumulating bacteria achieved complete phosphate removal in two different systems: an anaerobic-anoxic sequencing batch reactor and an anaerobic-aerobic sequencing batch reactor. this result shows that phosphate-accumulating bacteria in the a2 sbr can use nitrate as terminal electron acceptor instead of oxygen. phosphate-accumulating bacteria accumulated phosphate with a rates between 30 and 70 mg p/l/h in the a/o sbr and between 15 and 32 mg p/l/h in the a2 sbr. twenty ...19999841775
construction of a physical and preliminary genetic map of aeromonas hydrophila jmp636.a physical and preliminary genetic map of the aeromonas hydrophila jmp636 chromosome has been constructed. the topology of the genome was predicted to be circular as chromosomal dna did not migrate from the origin during pfge unless linearized by s1 nuclease. cleavage of the chromosome with paci and pmei produced 23 and 14 fragments, respectively, and enabled calculation of the genome size at 4.5 mb. digestion of the chromosome with i-ceui produced 10 fragments, indicating that 10 rrl (23s) gene ...19989846744
mobile drug resistance genes among fish bacteria. 19989850686
transcriptional regulation and organization of the dcua and dcub genes, encoding homologous anaerobic c4-dicarboxylate transporters in escherichia coli.the dcua and dcub genes of escherichia coli encode homologous proteins that appear to function as independent and mutually redundant c4-dicarboxylate transporters during anaerobiosis. the dcua gene is 117 bp downstream of, and has the same polarity as, the aspartase gene (aspa), while dcub is 77 bp upstream of, and has the same polarity as, the anaerobic fumarase gene (fumb). to learn more about the respective roles of the dcu genes, the environmental and regulatory factors influencing their exp ...19989852003
[bacteriological quality of spring and well water in yaounde (cameroon)].the drinking water supply in yaounde, cameroon, often fails to meet demand and this leads many people to seek water from springs and wells the microbiological quality of which is unknown. we carried out a microbiological survey of five spring water points and ten wells. the fifteen study sites were located in various residential districts and were selected according to geographical location and number of users. microorganisms were isolated on specific culture media using the membrane filter tech ...19989854008
optimisation of the protocol for detection of aeromonas species in ready-to-eat salads, and its use to speciate isolates and establish their prevalence.aeromonas spp. are detected in more than 500 cases of gastrointestinal infection each year in england and wales. this study aimed to identify their prevalence in ready-to-eat salads, which are a potential source of aeromonas infection. the protocol for isolation of mesophilic aeromonas spp. from salads was optimised. using the improved method, aeromonas spp were isolated from 19 of 25 samples (25 g) of ready-to-eat salad products. aeromonas organisms were counted, isolates were identified to spe ...19989854886
the risk to public health of aeromonas in ready-to-eat salad products.mesophilic aeromonas spp. are isolated regularly from cases of gastrointestinal infection and have markers indicative of enteropathogenicity. is aeromonas, which is present in a large proportion of ready-to-eat salads, actually a gastrointestinal pathogen? isolates of mesophilic aeromonas from salads were characterised in terms of their ability to grow at refrigeration temperatures over the given shelf life and by the presence of markers of potential virulence. the major phenospecies present in ...19989854887
purification and characterization of enterotoxin produced by aeromonas sobria.we purified the toxin of aeromonas sobria capable of inducing a positive response in the mouse intestinal loop assay. the purified toxin showed a positive response not only in the loop assay but also in a hemolytic assay. subsequently, we cloned the toxin gene and demonstrated that the product of this gene possessed both hemolytic and enterotoxic activities. these results showed that the enterotoxin of a. sobria possesses hemolytic activity. nucleotide sequence determination of the toxin gene an ...19989858466
symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations.hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. infections by aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered. the associated infections demonstrate the need for a better understanding of the digestive tract flora of h. medicinalis. early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of bl ...19999864188
isolation of aeromonas salmonicida in association with purple-pigmented bacteria in sediment from a scottish loch.aeromonas salmonicida was recovered in close association with an unidentified purple-pigmented organism, which was isolated from sediment in a scottish loch during november (1997) and february (1998). however, there has not been any evidence of a. salmonicida infections, specifically furunculosis, associated with the fish in this loch.19989871353
bacterial resistance to ultrasonic waves under pressure at nonlethal (manosonication) and lethal (manothermosonication) temperatures.the decimal reduction times of streptococcus faecium, listeria monocytogenes, salmonella enteritidis, and aeromonas hydrophila corresponding to heat treatment at 62 degrees c were 7.1, 0.34, 0.024, and 0.0096 min, and those corresponding to manosonication treatment (40 degrees c, 200 kpa, 117 microm) were 4.0, 1.5, 0.86, and 0.90 min, respectively. the manosonication decimal reduction times of the four species investigated decreased sixfold when the amplitude was increased from 62 to 150 microm ...19999872795
inhibition of streptomyces griseus aminopeptidase and effects of calcium ions on catalysis and binding--comparisons with the homologous enzyme aeromonas proteolytica aminopeptidase.streptomyces griseus aminopeptidase is a zinc metalloenzyme containing 2 mol zinc/mol protein, similar to the homologous enzyme aeromonas proteolytica aminopeptidase. in addition, a unique ca2+-binding site has been identified in the streptomyces enzyme, which is absent in the aeromonas enzyme. binding of ca2+ enhances stability of the streptomyces enzyme and modulates its activity and affinity towards substrates and inhibitors in a structure-dependent manner. among the three hydrophobic 4-nitro ...19989874195
a collagen-binding protein produced by aeromonas hydrophila.the gastrointestinal pathogen aeromonas hydrophila strain a186 produces a collagen-binding protein (cnbp) which is found extracellularly and loosely associated with the cell surface. the cell-associated cnbp was purified by sequential ammonium sulphate precipitation, size-exclusion chromatography and ion-exchange chromatography, or by sequential ammonium sulphate precipitation and affinity chromatography with collagen-sepharose. the purified cnbp was homogeneous in sds-page, and had a mol. wt of ...19989879942
a cluster of cases of infections due to aeromonas hydrophila revealed by combined rapd and eric-pcr.two polymerase chain reaction (pcr)-based methods were used for epidemiological typing of aeromonas hydrophila. random amplification of polymorphic dna (rapd) and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) were applied to an outbreak involving seven patients. the epidemiological situation appeared complex; with the exception of two clinical isolates, all gave unique patterns with both techniques. these methods demonstrated nosocomial transmission in one unit and permitted the ...19989879968
characterisation of a type iv pilus produced by aeromonas caviae.a pilus produced by a clinical isolate of aeromonas caviae (strain ca195) was purified and partially characterised. the mr of the pilin was estimated to be 23 kda by sds-page. its n-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type iv pili purified from other aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. bundle-forming pili, often with a polar location, were seen on the surface of strain ca19 ...19989879972
virulence factors of aeromonads--an emerging food borne pathogen problem.aeromonas spp. possess a number of virulence properties which are considered responsible for intestinal and extra-intestinal infections in human beings and also for a wide variety of infections in animals. the paper discusses current status of colonization and toxic factors of aeromonas spp., especially in relation to food and biochemical markers. future research needs are also identified.19989914671
aeromonas hydrophila associated with a severe outbreak of infection in farmed rabbits.an outbreak of infection with a high rate of mortality has been detected in an industrial rabbitry in spain. aeromonas hydrophila was isolated in pure culture from liver, lung, heart and spleen of ill and moribund animals. all the isolates belonged to o:11 serogroup, autogglutinated after boiling, were resistant to the bactericidal action of the fresh rabbit serum and did not agglutinate in acriflavine. they were producers of hemolysins and proteases but were not enterotoxigenic.19989916296
[meningitis by aeromonas sobria following cranio-encephalic traumatism]. 19989919002
co-expression of polyhydroxyalkanoate synthase and (r)-enoyl-coa hydratase genes of aeromonas caviae establishes copolyester biosynthesis pathway in escherichia coli.polyhydroxyalkanoate biosynthesis genes of aeromonas caviae were expressed in escherichia coli ls5218 (fadr atoc(con)), and the polyhydroxyalkanoate-producing ability of the recombinants was investigated. a ls5218 strain harboring only phacac (polyhydroxyalkanoate synthase gene) did not accumulate any polyhydroxyalkanoate from dodecanoate in spite of the existence of translated polyhydroxyalkanoate synthase protein, whereas co-expression phacac and phajac ((r)-specific enoyl-coa hydratase gene) ...19999919653
purification and characterization of an aeromonas caviae metalloprotease that is related to the vibrio cholerae hemagglutinin/protease.a zinc metalloprotease (ap34) from aeromonas caviae was purified by ammonium sulfate precipitation and subsequent gel filtration through sephadex g-100 and sephadex g-50 superfine. the molecular mass was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 34 kda. the protease showed maximum activity at ph 7.0 and was stable at 60 degrees c. ap34 was completely inactivated by edta and zincov. the n-terminal amino acid sequence of ap34 showed a high degree of homology with ...19999919673
prevalence and characteristics of aeromonas species isolated from processed channel catfish.from august 1994 to may 1995, 238 channel catfish fillets collected from three processing plants in the mississippi delta at four time periods were tested for the presence of aeromonas species. identification of aeromonas spp. was accomplished using an automated vitek bioassay system with gram-negative and nonfermenter cards. approximately 36.1% were positive for a. hydrophila, 35.7% for a. sobria, and 10.9% for a. caviae. all three aeromonas spp. were found in all three processing plants, and t ...19999921825
quantitative pcr for listeria monocytogenes with colorimetric detection.an enzyme-linked immunosorbent assay (elisa)-mediated polymerase chain reaction (pcr) technique was developed to detect and quantify listeria monocytogenes in food products. the bacterial dna was extracted from artificially contaminated food and co-amplified with a synthetic internal standard (is) using primers specific for the target gene coding for the invasive-associated protein (i.a.p.), a virulence factor of l. monocytogenes (i.a.p.) or is in the presence of fluorescein-dutp pcr products we ...19999921826
the cytotoxic enterotoxin of aeromonas hydrophila is aerolysin. 19999925450
resistance to antibiotic and heavy metals of motile aeromonads from chilean freshwater.in this work the resistance of 172 motile aeromonas isolates recovered from raw drinking water supplies (56), irrigation waters (60) and runoff waters receiving sewage (56), to some antibiotics and heavy metals was investigated by agar diffusion and agar dilution methods. a high proportion of isolates from all water sources showed resistance to carbenicillin, erythromycin, streptomycin, cephradine and cadmium, and susceptibility to chloramphenicol, kanamycin, gentamicin, tetracycline, nalidixic ...19989926432
an in-vitro evaluation of the activity of chlorine against environmental and nosocomial isolates of aeromonas hydrophila.a chlorine preparation was tested against 34 strains of aeromonas hydrophila isolated from a hospital water facility and patients with nosocomial infections in a french medical school hospital. in-vitro bactericidal activity was determined by a macro- and micromethod, using specific interfering substances over a range of dilutions and temperatures, after 1 and 5 minute exposure times. a 10(5)-fold reduction in the challenge inoculum was taken as the criterion of efficacy. results obtained by bot ...19999949964
antimicrobial susceptibility patterns of aeromonas jandaei, a. schubertii, a. trota, and a. veronii biotype veronii.fifty-six isolates of four aeromonas species, which have been documented as causative agents of human infections or isolated from human clinical specimens, were subjected to antimicrobial susceptibility testing using a microscan walkaway conventional (overnight incubation) gram-negative panel. the four species tested and the number of isolates of each were as follows: aeromonas jandaei, 17; a. schubertii, 12; a. trota, 15; and a. veronii biotype veronii, 12. all isolates of a. trota were suscept ...19999986836
an attempt to use selected strains of bacteria adapted to high concentrations of petroleum oil to increase the effective removal of petroleum products in excess activated sludge in laboratory conditions.forty two strains of bacteria were isolated from excess activated sludge from petroleum wastewater treatment plant. the strains were identified and classified to the following groups: enterobacteriaceae (7 strains), anitratum (3 strains), pseudomonas (13 strains), micrococcus (12 strains), comamonas (2 strains), xanthomonas (2 strains), achromobacter (1 strain) and vibrio-aeromonas (1 strain). one of the isolates was found to be a yeast strain. following preliminary selection ten strains, showin ...19989990713
telomerase reverse transcriptase gene is a direct target of c-myc but is not functionally equivalent in cellular transformation.the telomerase reverse transcriptase component (tert) is not expressed in most primary somatic human cells and tissues, but is upregulated in the majority of immortalized cell lines and tumors. here, we identify the c-myc transcription factor as a direct mediator of telomerase activation in primary human fibroblasts through its ability to specifically induce tert gene expression. through the use of a hormone inducible form of c-myc (c-myc-er), we demonstrate that myc-induced activation of the ht ...199910022128
genetic characterization of a new type iv-a pilus gene cluster found in both classical and el tor biotypes of vibrio cholerae.the vibrio cholerae genome contains a 5.4-kb pil gene cluster that resembles the aeromonas hydrophila tap gene cluster and other type iv-a pilus assembly operons. the region consists of five complete open reading frames designated pilabcd and yace, based on the nomenclature of related genes from pseudomonas aeruginosa and escherichia coli k-12. this cluster is present in both classical and el tor biotypes, and the pila and pild genes are 100% conserved. the pila gene encodes a putative type iv p ...199910024587
destabilase complexes--natural liposome produced by medicinal leeches hirudo medicinalis.electrophoretic analysis of destabilase preparation demonstrates the presence of protein combinations with mw 12.3, 25 and 50 kd. fraction (mw 12.3 d) is a monomer of destabilase aggregation having properties of micellar proteins and represents a stable lipid-protein complex, where the role of lipid component is played by the stable analogue of prostacyclin (mw 391 d). the synthesis of a low molecular fraction of destabilase is fulfilled with bacteria--symbiont of leeches aeromonas hydrophila. w ...199910027095
resistance of paroxysmal nocturnal hemoglobinuria cells to the glycosylphosphatidylinositol-binding toxin aerolysin.paroxysmal nocturnal hemoglobinuria (pnh) is a clonal stem cell disorder caused by a somatic mutation of the piga gene. the product of this gene is required for the biosynthesis of glycosylphosphatidylinositol (gpi) anchors; therefore, the phenotypic hallmark of pnh cells is an absence or marked deficiency of all gpi-anchored proteins. aerolysin is a toxin secreted by the bacterial pathogen aeromonas hydrophila and is capable of killing target cells by forming channels in their membranes after b ...199910029605
the effect of diet on aerobic bacterial flora associated with intestine of arctic charr (salvelinus alpinus l.).in order to extend the knowledge on the possible effect of diet on the gastrointestinal microbial community of fish, arctic charr (salvelinus alpinus l.) were fed diets containing high (23.7%) and low (6.4%) levels of carbohydrate. the number of viable aerobic and facultative aerobic bacteria associated with the digestive tract were not influenced by dietary regimen. a wide range of bacterial species was isolated, and the predominant bacterial species of both rearing groups were identified as st ...199910030010
note: susceptibility to chlorine of aeromonas hydrophila strains.the susceptibility of five aeromonas hydrophila strains and one escherichia coli strain to chlorine was studied under carefully controlled laboratory conditions. of the aer. hydrophila strains, two were from untreated water, two from tap water (immediately downstream of a water treatment plant) and one from the dsm collection. the study included disinfectant concentration (0.1, 0.2 and 0.5 mg l-1), ph (6, 7 and 8) and temperature (4, 21 and 32 degrees c) as controlled variables. the results indi ...199910030019
the degradation of sodium o,o-diethyl dithiophosphate by bacteria from metalworking fluids.the breakdown of sodium o,o-diethyl dithiophosphate (o,o-diethyl phosphorodithioate) by four bacterial strains (tentatively identified as strains of aeromonas, pseudomonas, flavobacterium and bacillus) isolated from contaminated metalworking fluids was shown to involve the successive formation of ethanol, aldehyde and orthophosphate. an acid phosphodiesterase was identified in cell-free extracts that was five- to sevenfold enhanced in specific activity in bacteria grown on o,o-diethyl dithiophos ...199910030034
expression and properties of an aerolysin--clostridium septicum alpha toxin hybrid protein.aerolysin is a bilobal channel-forming toxin secreted by aeromonas hydrophila. the alpha toxin produced by clostridium septicum is homologous to the large lobe of aerolysin. however, it does not contain a region corresponding to the small lobe of the aeromonas toxin, leading us to ask what the function of the small lobe is. we fused the small lobe of aerolysin to alpha toxin, producing a hybrid protein that should structurally resemble aerolysin. unlike aerolysin, the hybrid was not secreted whe ...199910048023
ulcerative dermatitis caused by aeromonas salmonicida spp. salmonicida in farmed atlantic salmon in british columbia. 199810051960
the production of a novel carbapenem-hydrolysing beta-lactamase in aeromonas veronii biovar sobria, and its association with imipenem resistance. 199810052912
dna probes specific for aeromonas hydrophila (hg1).aeromonas hydrophila (hg1)-specific rapd-pcr fragments were investigated for their potential as dna probes. from 20 rapd-pcr fragment bands, it was found that two were specific to all isolates of aeromonas hydrophila (hg1) tested. cloning and nucleotide sequence determination of one of these bands showed that co-migration of similar sized amplicons had occurred and that this band (designated '7e') contained at least four fragments of different sequences. three of these individual amplicons had a ...199910063616
abdominal ascites in electric eels (electrophorus electricus) associated with hepatic hemosiderosis and elevated water ph.six electric eels (electrophorus electricus) from various centers that house aquatic organisms presented clinically with abdominal distension following prolonged exposure to elevated environmental ph. postmortem examination revealed marked ascites. culture of the abdominal fluid from three of the eels yielded either aeromonas hydrophila or citrobacter freundii, which were most likely secondary invaders. histopathology showed marked iron accumulation in both hepatocytes and hepatic macrophage agg ...199810065849
haemolytic-uraemic syndrome in an adult male with aeromonas hydrophila enterocolitis. 199910069207
mortality of captive canadian toads from basidiobolus ranarum mycotic dermatitis.twenty-six adult free-ranging canadian toads (bufo hemiophrys) were collected from northeastern north dakota (usa) during the last week of august 1994 and placed in captivity. during late december and january 1995, 21 canadian toads died. clinical signs included increased time sitting in water bowls, darkened dorsal skin, constant arching of their backs, and hyperemia and sloughing of ventral epidermis. the condition progressively worsened until death occurred within 5 to 7 days after onset of c ...199910073347
identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr.molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ...199910074520
[aeromonas septicemia]. 199910088391
peroxide-inducible catalase in aeromonas salmonicida subsp. salmonicida protects against exogenous hydrogen peroxide and killing by activated rainbow trout, oncorhynchus mykiss l., macrophages.aeromonas salmonicida subsp. salmonicida expresses a single cytoplasmically located catalase which was found to be inducible by exposure to 20 microm hydrogen peroxide in mid-exponential phase resulting in a 4 fold increase in activity. subsequent exposure to 2 mm peroxide in late-exponential/early-stationary phase resulted in further induction of catalase activity which increased to 20 fold higher levels than those found in uninduced cultures. exponentially induced cultures were protected again ...199910089155
the type iv aeromonas pilus (tap) gene cluster is widely conserved in aeromonas species.nothing is known regarding the expression or function of the type iv aeromonas pilus (tap), which was recently identified following the cloning of a pilus biogenesis gene cluster (tapabcd). as a first step to determine the possible significance of tap for aeromonas virulence, the distribution of the tapa and tapd genes in hybridization group reference strains and clinical (n=42) and environmental (n=29) isolates was determined. homologues of tapa and tapd were present in all strains tested. hybr ...199910090854
catecholamine enhancement of aeromonas hydrophila growth.several species of bacteria have been shown to respond to the administration of norepinephrine and other catecholamines with increased growth (in culture) and virulence. in this study, we examined the effects of catecholamines on the growth of cultures of aeromonas hydrophila, a gram-negative bacillus found in brackish water. bacterial cultures were maintained in tryptic soy both, then washed free of medium and transferred to a bovine serum-supplemented minimal salts medium. treatment of a. hydr ...199910090855
small subunit rrna gene sequences of aeromonas salmonicida subsp. smithia and haemophilus piscium reveal pronounced similarities with a. salmonicida subsp. salmonicida.the small subunit ribosomal rna (ssu rrna) encoding genes from reference strains of aeromonas salmonicida subsp. smithia and haemophilus piscium were amplified by polymerase chain reaction and cloned into escherichia coli cells. almost the entire ssu rrna gene sequence (1505 nucleotides) from both organisms was determined. these dna sequences were compared with those previously described from a. salmonicida subsp. salmonicida, subsp. achromogenes and subsp. masoucida. this genetic analysis revea ...199910092980
appearance of imp-1 metallo-beta-lactamase in europe. 199910093989
copper exposure reduced the resistance of the catfish saccobranchus fossilis to aeromonas hydrophila infection. 199910094734
aeromonas species isolated from medicinal leeches.aeromonas hydrophila infections are a recognized complication of the use of medicinal leeches. the authors performed an experiment designed to find a safe and practical way to sterilize the leech gut of pathogenic organisms. leeches were incubated for a 12-hour period in solutions of antibiotic effective against a. hydrophila. the incubations in the antibiotic solutions failed to eradicate pathogenic bacteria from the gut of the leeches. the authors examined cultures of bacteria isolated from th ...199910096618
aeromonas hydrophila isolated from cases of bovine seminal vesiculitis in south brazil. 199910098696
secretion of hemolysin of aeromonas sobria as protoxin and contribution of the propeptide region removed from the protoxin to the proteolytic stability of the toxin.the sequence at the amino terminus region of the hemolysin ofaeromonas sobria is homologous with that of aerolysin of a. hydrophila. however, there is no homology between the two toxins in the sequence at the carboxy terminal region. it has been shown that aerolysin is secreted into culture supernatant as a protoxin. this proaerolysin is activated by the proteolytic removal of a carboxy terminal peptide. however, the role of the carboxy terminal region, which is removed in the activation process ...199910100744
purification and characterization of cold-active l-glutamate dehydrogenase independent of nad(p) and oxygen.l-glutamate dehydrogenase (gldh) independent of nad(p) and oxygen was first obtained from the psychrotrophic bacterium aeromonas sp. l101, originally isolated from the organs of salmon (oncorhynchus keta). gldh was purified by a series of chromatography steps on deae-sepharose, superdex 200pg, q-sepharose, cm-sepharose, and phenyl-sepharose. the purified protein was determined to have a molecular mass of 110 kda and a pi of 5.7. maximum activity was obtained at 55 degrees c and ph 8.5. the activ ...199910101290
distributional patterns of bacterial diarrhoeagenic agents and antibiograms of isolates from diarrhoeaic and non-diarrhoeaic patients in urban and rural areas of nigeria.to determine the prevalence of bacteria that could cause diarrhoea in stool specimens of individuals with and without diarrhoea in both urban and rural areas of nigeria. to ascertain the antibiotic susceptibilities of the bacterial diarrhoeagenic agents isolated. to document the predominant signs and symptoms associated with the various bacterial agents of diarrhoea.199810101427
structure of in31, a blaimp-containing pseudomonas aeruginosa integron phyletically related to in5, which carries an unusual array of gene cassettes.the location and environment of the acquired blaimp gene, which encodes the imp-1 metallo-beta-lactamase, were investigated in a japanese pseudomonas aeruginosa clinical isolate (isolate 101/1477) that produced the enzyme. in this isolate, blaimp was carried on a 36-kb plasmid, and similar to the identical alleles found in serratia marcescens and klebsiella pneumoniae clinical isolates, it was located on a mobile gene cassette inserted into an integron. the entire structure of this integron, nam ...199910103196
biochemical characterization of the pseudomonas aeruginosa 101/1477 metallo-beta-lactamase imp-1 produced by escherichia coli.the blaimp gene coding for the imp-1 metallo-beta-lactamase produced by a pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a t7 expression system in escherichia coli bl21 (de3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. the structural and functional properties of the enzyme purified from e. coli were identical to those of the enzyme produced by p. aeruginosa. the imp-1 metallo-beta-lactamase exhibits a broad-spectrum ...199910103197
signature region within the 16s rdna sequences of aeromonas popoffii.to identify a group of eight aeromonas strains of our collection showing ribotyping patterns similar to those described for the species aeromonas popoffii, 16s rrna gene sequence analysis was performed. results were in agreement with the dna binding values, and allowed the identification of a 'signature region' differentiating the a. popoffii strains from all other members of the genus aeromonas.199910188252
the suppressive excretory-secretory product of trichobilharzia ocellata: a possible factor for determining compatibility in parasite-host interactions.factors which may determine trematode-snail interactions were assessed in the present study. compatibility was examined using a bacterial clearance assay to detect the modulatory effects of both compatible and incompatible trematode infections on the activity of haemocytes from lymnaea stagnalis, during the early stages of infection. exposure to and injection with trichobilharzia ocellata, a compatible trematode, or the incompatible schistosoma mansoni, resulted in modulation of haemocyte activi ...199710190175
unusual case of aeromonas sobria cellulitis associated with the use of leeches. 199910192721
evaluation of xylose-galactosidase medium, a new plate for the isolation of salmonella, shigella, yersinia and aeromonas species. 199910192724
effect of florfenicol on the immune response of rainbow trout (oncorhynchus mykiss).florfenicol, a drug effective against several bacterial diseases of fish, was tested for possible immunomodulatory effects. the aim of the study was to follow the kinetics of the immune response after vaccination with simultaneous oral antibiotic treatment. the fish were immunised with a commercial oil-based divalent (furunculosis/vibriosis) vaccine and were simultaneously given oral antibiotic treatment. the specific immune response was monitored by analysing the levels of specific antibodies w ...199910206200
identification of aeromonas trota (hybridization group 13) by amplification of the aerolysin gene using polymerase chain reaction.aeromonas trota is recognized as an important enteropathogen, and its haemolysin (aerolysin) is purported to be one of the virulence factors. rapid detection and identification of a. trota is important for early and specific diagnosis of the infectious diseases that it causes. synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) technique to amplify a species-specific sequence of the aera gene, which encodes the aerolysin of a. trota. a dna fragment of 622 bp was ampl ...199910208799
leech-borne serratia marcescens infection following complex hand injury.leeches are commonly used in the postoperative course of plastic surgical operations where there is venous congestion in a pedicled or free flap. they provide a temporary relief to venous engorgement whilst venous drainage is re-established. it is known that leeches can carry aeromonas hydrophila infection, and a second or third generation cephalosporin antibiotic has traditionally been given as prophylaxis against infection. we report a new observation that leeches can carry serratia marcescens ...199810209471
prevalence, in-vitro secretory activity, and cytotoxicity of aeromonas species associated with childhood gastroenteritis in chennai (madras), india.an investigation on the prevalence of aeromonas in gastrointestinal illnesses of pediatric inpatients 1 month to 3 years of age was conducted from february 1997 through january 1998 in madras. sixteen aeromonas spp. were isolated from 11 male and five female children among the 341 pediatric inpatients suffering from acute diarrhoea. a. caviae, which was isolated from nine cases, was found to be the most predominant isolate, followed by a. veronii biovar sobria, isolated from six cases, and a. hy ...199810211427
[bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996].during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ...199910213987
thermostable aminopeptidase from pyrococcus horikoshii.from the genome sequence data of the thermophilic archaeon pyrococcus horikoshii, an open reading frame was found which encodes a protein (332 amino acids) homologous with an endoglucanase from clostridium thermocellum (42% identity), deblocking aminopeptidase from pyrococcus furiosus (42% identity) and an aminopeptidase from aeromonas proteolytica (18% identity). this gene was cloned and expressed in escherichia coli, and the characteristics of the expressed protein were examined. although endo ...199910218575
changes in serum concentration of a serum amyloid p-like pentraxin in atlantic salmon, salmo salar l., during infection and inflammation.a serum amyloid p-like pentraxin has been isolated from atlantic salmon. salmo salar l., based on its calcium dependent binding to agarose. the subunits of approximately 37 kda were all glycosylated and when covalently linked together formed a pentamer with disulphide bonds between all subunits. a specific rabbit antiserum raised against the pentameric form was used to follow changes in serum levels of the salmon pentraxin during infection with aeromonas salar and inflammation induced by escheri ...199910220069
the gene encoding a periplasmic deoxyribonuclease from aeromonas hydrophila.a gene encoding a deoxyribonuclease, dnsh, was cloned from aeromonas hydrophila jmp636. the predicted mature protein was very similar to the previously described extracellular dns from this organism and an n-terminal region corresponding to a large putative signal sequence was predicted for the jmp636 protein. inactivation of dnsii demonstrated that the dnsh protein was not present extracellularly in this strain. as dnsh degraded plasmid dna and was believed to have a periplasmic location, a dns ...199910220879
[rapidly fatal prognosis caused by aeromonas sobria septic shock in a patient with myelodysplastic syndrome]. 199910222675
fish macrophages express a cyclo-oxygenase-2 homologue after activation.in mammals, the increased generation of prostaglandins (pg) during the onset of inflammatory responses and activation of immune cell types has been attributed to the induction of a novel cyclo-oxygenase (cox) isoform, termed cox-2, which is distinct from the well-characterized constitutive activity (cox-1). goldfish (carassius auratus) macrophages exposed to bacterial lipopolysaccharide and leucocyte-derived macrophage-activating factor(s) showed a significant increase in the generation of the m ...199910229670
expression and export of pseudomonas putida ntu-8 creatinase by escherichia coli using the chitinase signal sequence of aeromonas hydrophila.the gene for the creatinase from pseudomonas putida ntu-8 was sequenced and revealed an open reading frame (orf) of 1209 base pairs encoding a polypeptide of 403 amino acids with a calculated molecular weight (m(r)) of 45,691. the deduced amino acid sequence is very similar to that of the creatinase of pseudomonas putida and flavobacterium sp. an overproduction system for the chitinase signal peptide--creatinase hybrid gene was constructed by using the pqe-51 expression vector in e. coli jm109. ...199810230521
monitoring the conjugal transfer of plasmid rp4 in activated sludge and in situ identification of the transconjugants.a gfpmut3b-tagged derivative of broad host-range plasmid rp4 was used to monitor the conjugative transfer of the plasmid from a pseudomonas putida donor strain to indigenous bacteria in activated sludge. transfer frequencies were determined to be in the range of 4 x 10(-6) to 1 x 10(-5) transconjugants per recipient. in situ hybridisation with fluorescently labeled, rrna-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.199910234817
distribution of two hemolytic toxin genes in clinical and environmental isolates of aeromonas spp.: correlation with virulence in a suckling mouse model.previous studies have shown that two hemolytic toxins, hlya and aera, contribute to the virulence of aeromonas hydrophila. a survey was performed to gauge the distribution of hlya and aera genes in clinical and environmental aeromonas isolates. for a. hydrophila, a. veronii biotype sobria and a caviae, 96%, 12% and 35% of strains, respectively, were hlya positive, whereas, 78%, 97%, 41%, respectively, were aera positive. all virulent a. hydrophila isolates were hlya+ aera+. this genotype was mos ...199910234831
surgeon a clue in puzzle of unusual outbreak. 197910242102
nosocomial acquisition of aeromonas hydrophila. 197910245973
emergence of carbapenem-hydrolyzing enzymes in acinetobacter baumannii clinical isolates. 199910325396
important aspects of the colonization of central venous catheter.this study comprises five different kind of venous central catheters, 103 in total, made of polyurethane tecoflex, polyurethane vialon, ptfe and pvc, and the influence of their raw material on the microbial colonization. patients age and sex, besides their clinical conditions, were taken into account, and neither considered as a sample vicious, nor associated with colonization. when the tips of the catheters were asseptically inoculated in tryptic soy broth and tioglicolate, colonization was det ...199910326311
effects of chromium on humoral and cell-mediated immune responses and host resistance to disease in a freshwater catfish, saccobranchus fossilis (bloch).the effects of subtoxic levels of cr on humoral and cell-mediated immune responses, blood parameters, susceptibility to bacterial (aeromonas hydrophila) infection, and macrophage activity in the freshwater air-breathing asian catfish, saccobranchus fossilis, during a 28-day exposure were examined by a static bioassay test procedure. at 0.1, 1.0, and 3.2 mg / liter cr, dose-dependent cr accumulation in kidney, liver, and spleen was observed at the end of the experiment. chromium exposure caused a ...199910330315
rearing stable fly larvae (diptera: muscidae) on an egg yolk medium.the growth and survival of stomoxys calcitrans (l.) larvae on egg yolk medium inoculated with bacteria isolated from a colony of stable flies was evaluated. five species of bacteria--acinetobacter sp., aeromonas sp., empedobacter breve (holmes & owen), flavobacterium odoratum stutzer, and serratia marcescens bizio--were identified according to fatty acid profiles using a microbial identification system. larvae failed to develop on uninoculated plates, confirming that bacteria are required to com ...199910337112
presumed clostridial and aerobic bacterial infections of the cornea in two horses.microscopic examination of gram-stained tissue specimens collected from severe corneal ulcers in 2 horses revealed large gram-positive rods suggestive of clostridium spp. clostridium perfringens was isolated from specimens collected from horse 1; anaerobic organisms were not detected in specimens from horse 2. aerobic bacterial culture revealed aeromonas hydrophila and enterobacter cloacae in specimens collected from horses 1 and 2, respectively. an insect exoskeleton was presumed to be the unde ...199910340080
enumeration and confirmation of aeromonas hydrophila, aeromonas caviae, and aeromonas sobria isolated from raw milk and other milk products in northern greece.a total of 138 raw cow's and 57 raw ewe's milk samples; 80 pasteurized cow's milk samples; 39 anthotyros cheese, 36 manouri cheese, and 23 feta cheese samples; and 15 rice pudding samples were examined for the presence and any countable population of aeromonas species. twenty-two (15.9%) of the 138 cow's milk samples analyzed were contaminated with a. hydrophila. in 13 of these samples, populations of 3.0x10(2) to 5.0x10(3) cfu/ml were counted in starch ampicillin agar (saa). eighteen cow's milk ...199910340665
[spontaneous bacterial peritonitis and sepsis due to aeromonas hydrophila]. 199910349797
kinetic behavior of some polyphosphate-accumulating bacteria isolates in the presence of nitrate and oxygen.this paper studies the phosphate uptake by pure cultures of aeromonas hydrophila, klebsiella oxytoca, agrobacterium tumefaciens, and aquaspirillum dispar in the presence of both nitrate and oxygen. it is shown that species were able to respire both electron acceptors for phosphate accumulation. a. tumefaciens and a. dispar accumulated overall phosphate both in oxic and anoxic culture conditions, whereas a. hydrophila and k. oxytoca eliminated overall phosphate only in oxic conditions. a. dispar ...199910355118
significance of cryptosporidium in acute diarrhoea in north-eastern india.in a hospital-based study, stool samples from 2095 patients of all ages were examined for different fungal, protozoal and bacterial enteropathogens over a period of 2 years (july 1994-june 1996). cryptosporidium was detected in 151 specimens (7.2%) and was the third commonest pathogen found. the highest prevalence of this organism was in the group aged 16-45 years and during the rainy months (july-oct.). diarrhoea caused by the protozoon was of mild to moderate severity and features of dysentery ...199910359300
evaluation of statens serum institut enteric medium for detection of enteric pathogens.the efficacy of the statens serum institut (ssi) enteric medium for isolation and direct identification of enteric pathogens was evaluated. six different biochemical reactions can be read by using the ssi enteric medium, allowing direct identification of a range of enteric pathogens. all 248 gram-negative bacterial species that were tested grew on the ssi enteric medium. only 10 of 248 bacteria (4%) showed discrepant results in the biochemical reactions, and none of these were enteric pathogens. ...199910364603
lapstatin, a new aminopeptidase inhibitor produced by streptomyces rimosus, inhibits autogenous aminopeptidases.lapstatin, a low-molecular-weight aminopeptidase inhibitor, was purified to homogeneity from streptomyces rimosus culture filtrates. the purification procedure included extraction with methanol, followed by chromatography on dowex 50wx4, ag50wx4, and hplc rp c18 columns. by amino acid analysis, mass spectrometry, and nmr spectroscopy, the structure of lapstatin was shown to be 3-amino-2-hydroxy-4-methylpentanoylvaline. lapstatin inhibited the extracellular leucine aminopeptidases from streptomyc ...199910369895
intramolecular chaperone and inhibitor activities of a propeptide from a bacterial zinc aminopeptidase.an aminopeptidase from aeromonas caviae t-64 was translated as a preproprotein consisting of three domains; a signal peptide (19 amino acid residues), an n-terminal propeptide (101 residues) and a mature region (273 residues). we demonstrated that a proteinase, which was isolated from the culture filtrate of a. caviae t-64, activated the recombinant pro-aminopeptidase by removal of the majority of the propeptide. using l-leu-p-nitroanilide as a substrate, the processed aminopeptidase showed a la ...199910377241
identification of inadequately cleaned equipment used in a sheep carcass-breaking process.aeromonads deposited on pork during a carcass-breaking process were recovered on hydrophobic grid membrane filters placed on ampicillin-dextrin-ethanol agar. isolates from 85 honey-yellow colonies from filters on that medium were aeromonas hydrophila (95%) or vibrio sp. (5%). presumptive aeromonads, coliforms, and escherichia coli in swab samples from product passing through a sheep carcass-breaking process were enumerated by hydrophobic grid membrane filtration techniques with a detection level ...199910382653
viable heterotrophic bacteria in water and sediment in 'mar piccolo' of taranto (ionian sea, italy).samples of water and sediment were collected from october 1996 to september 1997 in 'mar piccolo' of taranto (ionian sea, italy). mar piccolo is a semi-enclosed basin subject to pollution and receives a considerable amount of sewage and industrial waste. qualitative and quantitative analyses of the composition of the microbial flora were conducted on samples from six stations. the highest bacterial densities, in water and in sediment samples, were found in summer and the lowest, in autumn. among ...199910389241
identification of a pathogen-binding lectin in salmon serum.a mannose-binding lectin was isolated from the blood serum of atlantic salmon (salmo salar). analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and non-reducing conditions revealed a multimeric structure composed of 17000 mr subunits. hexosamine analysis and glycosidase digestion showed that the lectin is not glycosylated and amino acid analysis revealed no unusual compositional features. using ruthenium red staining, the lectin was shown to bind ca2+ ions. n-te ...199910390051
enhancement of anti-aeromonas salmonicida activity in atlantic salmon (salmo salar) macrophages by a mannose-binding lectin.we investigated the effects of a calcium-dependent mannose-binding lectin isolated from the serum of atlantic salmon on aeromonas salmonicida viability and the anti-a. salmonicida activity of atlantic salmon macrophages. in the absence of other factors, binding of this lectin at concentrations of 0.8, 4.0 and 20.0 ng ml(-1) to virulent a. salmonicida failed to significantly reduce (p> 0.05) cell viability. however, binding of the lectin to a. salmonicida did result in significant (p < or = 0.05) ...199910390056
isolation and haemolytic activity of aeromonas species from domestic dogs and cats.rectal swabs from 120 domestic dogs and 15 domestic cats were examined for aeromonas species using alkaline peptone water (ph 8.6) as the enrichment medium and blood agar containing 15 mg/l ampicillin as the plating medium. aeromonads were isolated from 13 (10.8%) dogs and from 1 (6.7%) cat. of the 14 aeromonads isolated in the present study only 9 were available for speciation and testing in the haemolysin assay. of these 5 were a. sobria (including one from a cat), 2 were a. hydrophila and 2 w ...199910391504
pseudo-outbreak of aeromonas hydrophila isolates related to endoscopy.over a two-month period there was a sudden increase in aeromonas hydrophila isolated from colon i.e., biopsies from patients without related symptoms. strains were studied by biotyping (api 20 ne, biomerieux), antibiotyping, plasmid analysis, sds-page of whole cell proteins and toxicity for cell cultures. all strains gave identical results. in particular sds-page of whole cell proteins showed an identical electrophoretic pattern for all the strains, and so all were considered to be of the same c ...199910392337
structure elucidation of sch 20562, a glucosidic cyclic dehydropeptide lactone--the major component of w-10 antifungal antibiotic.a novel bacterium designated as aeromonas sp. w-10 produces the antibiotic w-10 complex which comprises of two major and several minor components. the two major components from this complex, sch 20562 (1) and sch 20561 (1a), are of biological interest in view of their potent antifungal activity. the chemical degradation studies utilized for the assignment of structure 1 for sch 20562 are described here. some of the noteworthy diversity of structural features in this glucosidic cyclic dehydronona ...199910395274
structure elucidation of sch 20561, a cyclic dehydropeptide lactone--a major component of w-10 antifungal antibiotic.antibiotic w-10 is a fermentation complex produced by the bacterium aeromonas sp. w-10. the cyclic dehydropeptide lactones sch 20562 (1) and sch 20561 (2) are the major components of this fermentation complex and are of biological interest in view of their unique structural features and potent antifungal activity. the chemical degradation studies that were utilized in the assignment of structure 2 for sch 20561 are described here. the structure determination of 2 made use of the ozonolytic cleav ...199910395275
a study of infectious intestinal disease in england: microbiological findings in cases and controls.a study was undertaken to identify the microorganisms and toxins in stool specimens associated with infectious intestinal disease (iid) among cases in the community and presenting to general practitioners (gps) and in asymptomatic controls. population based cohorts were recruited from practice lists in 70 practices and followed for 26 weeks (cohort component). seven hundred and sixty-one cases of iid identified from the cohorts, 2893 cases who presented to gps in 34 of the practices (gp componen ...199910402744
biochemical characteristics & secretory activity of aeromonas species isolated from children with gastroenteritis in chennai.an attempt was made to delineate the phenotypic markers for the detection of enterotoxigenic strains of aeromonas. eighteen aeromonas species comprising one isolate of a. hydrophila, six isolates of a. sobria and 11 isolates of a. caviae were obtained from 379 children suffering from acute diarrhoea in chennai. nine of these isolates inclusive of three a. sobria and six a. caviae were found to produce secretory response in vitro in the rabbit intestinal mucosa mounted in the ussing chambers as r ...199910402760
recombinant light chain of human monoclonal antibody hb4c5 as a potentially useful lung cancer-targeting vehicle.recombinant lambda light chain of lung cancer-reacting human monoclonal antibody hb4c5 was expressed in escherichia coli. expression in bacteria ensured the generation of homogeneous light chain species devoid of activity-hampering n-linked glycosylation usually found in the light chain cdr-1 of hb4c5. molecular engineering was also employed to eliminate the c-terminal two amino acid residues, i.e., cys and ser, to prevent the formation of lambda light chain dimers which are less reactive than t ...199910405832
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