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occurrence, diversity and pathogenicity of mesophilic aeromonas in estuarine waters of the italian coast of the adriatic sea.a total of 208 strains of aeromonas were isolated by monthly sampling from two estuaries (one provided with, and the other devoid of a waste-water treatment system) on the italian coast of the adriatic sea between september 1994 and august 1995. biotyping at the species level allowed the identification of 96 strains (46%) as aer. caviae, 46 (22%) as aer. sobria, 33 (16%) as aer. hydrophila and 25 (12%) as aer. veronii. eight strains (4%) were regarded as unnamed aeromonads. aeromonas caviae was ...19989750281
the occurrence of aeromonas species in drinking water supplies of an area of the dolomite mountains, italy.a study was made of the occurrence of aeromonas spp. in drinking water supplies in a mountain area in northeast italy (the dolomites). on account of its location, the water in question is exposed to a low level of pollution and systematic chemical disinfection is not necessary. out of 7395 water samples analysed over a 3 year period, 1623 (21 x 95%) were found to be positive for aeromonas, with levels ranging from 1 to 240 cfu 100 ml-1; 72 x 4% of the strains were identified as aer. hydrophila, ...19989750299
fishtank water as a source of a rare case of aeromonas hydrophila septicaemia. 19989754216
molecular properties and activity of a carboxyl-terminal truncated form of xylanase 3 from aeromonas caviae w-61.aeromonas caviae w-61 produces five species of xylanases, xylanases 1, 2, 3, 4, and 5 [nguyen, v.d. et al., biosci. biotechnol. biochem., 56, 1708-1712 (1993) and appl. environ. microbiol., 57, 445-449 (1991)]. while preserving a purified xylanase 3 preparation from a. caviae in solution at 4 degrees c, the xylanase 3 was found to be proteolyzed to give a truncated form with a smaller molecular mass than that of the intact one. it appears likely that the truncated form of xylanase 3 was produced ...19989757562
distribution and life strategies of two bacterial populations in a eutrophic lakemonoclonal antibodies and epifluorescence microscopy were used to determine the depth distribution of two indigenous bacterial populations in the stratified lake plusssee and characterize their life strategies. populations of comamonas acidovorans px54 showed a depth distribution with maximum abundances in the oxic epilimnion, whereas aeromonas hydrophila pu7718 showed a depth distribution with maximum abundances in the anoxic thermocline layer (metalimnion), i. e., in the water layer with the h ...19989758799
x-ray structure of the znii beta-lactamase from bacteroides fragilis in an orthorhombic crystal form.beta-lactamases are extracellular or periplasmic bacterial enzymes which confer resistance to beta-lactam antibiotics. on the basis of their catalytic mechanisms, they can be divided into two major groups: active-site serine enzymes (classes a, c and d) and the znii enzymes (class b). the first crystal structure of a class b enzyme, the metallo-beta-lactamase from bacillus cereus, has been solved at 2.5 a resolution [carfi, pares, duée, galleni, duez, frère & dideberg (1995). embo j. 14, 4914-49 ...19989761816
enzyme characteristics of beta-d-galactosidase- and beta-d-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and escherichia coli.bacteria which were beta-d-galactosidase and beta-d-glucuronidase positive or expressed only one of these enzymes were isolated from environmental water samples. the enzymatic activity of these bacteria was measured in 25-min assays by using the fluorogenic substrates 4-methylumbelliferyl-beta-d-galactoside and 4-methylumbelliferyl-beta-d-glucuronide. the enzyme activity, enzyme induction, and enzyme temperature characteristics of target and nontarget bacteria in assays aimed at detecting colifo ...19989501441
antibacterial effects of n-sulfonated and n-sulfobenzoyl chitosan and application to oyster preservation.the antibacterial effects of sulfonated and sulfobenzoyl chitosans were evaluated and compared with that of 69% deacetylated chitosan (dd69 chitosan). minimal inhibitory concentrations of sulfonated chitosan (sc1, 0.63% sulfur content) against shigella dysenteriae, aeromonas hydrophila, salmonella typhimurium, and bacillus cereus were found to be lower than those of dd69 chitosan. a high sulfur content in sulfonated chitosan adversely influenced its antibacterial effect. sulfobenzoyl chitosan (s ...19989766062
mesophilic aeromonas strains from different serogroups: the influence of growth temperature and osmolarity on lipopolysaccharide and virulence.growth of mesophilic aeromonas sp. strains from serogroups o:13, o:33 and o:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (lps) and virulence of the strains tested, as we had previously reported for strains from serogroup o:34. the effect of osmolarity could be observed when the cells grew at 37 degrees c but not at 20 degrees c. purified lps from cells cultivated at 20 degrees c (high or low osmolarity) or at 37 degrees c at high osmolarity was smooth ...19989766240
an exeab complex in the type ii secretion pathway of aeromonas hydrophila: effect of atp-binding cassette mutations on complex formation and function.the energy-dependent secretion of aerolysin by aeromonas hydrophila requires the exea and exeb proteins. an 85 kda complex containing the two proteins was identified in wild-type cells but not in cells producing either protein alone. radiolabelling followed by cross-linking, immunoprecipitation and then reduction of the cross-links confirmed the presence of the two proteins in the same complex. the complex could also be extracted intact from cell membranes with non-ionic detergents. a g229d subs ...19989767591
diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: aeromonas, plesiomonas, campylobacter.the reactiviteisofstrains ofplesionmona shigeloides, campylobater jejuni, campylobacter coli and aeromonas species with some lectins namely soyabean agglutinin (sba), ricinus communis agglutinin (rca), peanut agllutinin (pna), cancanavalin-a (con-a), lima bean agglutinin (lma) and abrin were investigated using the microtitre plate method. the results obtained show that all stains of a. hydrophila, a. caviae, a. sobria and p. shigelloides reacted very strongly with pna and sba. all strains of c. ...199817581001
microbiological profile of raw nile water.with the increase of population on the nile banks, a remarkable increase in industrial, agricultural, human activities, and recreational activities have also occurred. the effluents of such activities are discharged directly into the nile or through some agricultural drains which finally discharge their wastes into the nile. thus, the microbiological monitoring is one of the main objectives to protect the river nile. this study was conducted to determine the microbiological profile of river nile ...199817217019
the effects of pollution on fish health.potentially harmful substances-e.g. pesticides, heavy metals and hydrocarbons-are often released into the aquatic environment. when large quantities of pollutants are released there may be an immediate impact as measured by large-scale sudden mortalities of aquatic organisms, e.g. fish kills resulting from contamination of waterways with agricultural pesticides. lower levels of discharge may result in an accumulation of the pollutants in aquatic organisms. the end results, which may occur long a ...199821182713
bacteriocin activity and probiotic activity of aeromonas media.three strains of aeromonas media (161, a164 and a199) were shown to be active in-vitro producers of bacteriocin-like inhibitory substances (blis). for example, the producer strain, aer. media a199, displayed antagonistic activity against all strains tested of aer. caviae, aer. hydrophila, aer. salmonicida, aer. veronii var. sobria, listonella anguillarum, photobacterium damsella, eight species of vibrio and yersinia ruckeri. because of this wide-ranging activity against fish/shellfish pathogens, ...199821182714
new developments in meat starter cultures.meat starter cultures containing one or more strains of lactic acid bacteria,actinobacteria, staphylococci,halomonas elongata, aeromonas spec., and moulds or yeasts are widely in practical use. the progress in microbial systematic has led to changes in the taxonomy of familiar bacterial species which are described. studies of flavour genesis led to the identification of the contribution of the enzyme activities endogenously present in the meat matrix as well as of those exerted by the starter cu ...199822060705
dissimilatory nitrate reduction in anaerobic sediments leading to river nitrite accumulation.recent studies on northern ireland rivers have shown that summer nitrite (no(inf2)(sup-)) concentrations greatly exceed the european union guideline of 3 (mu)g of n liter(sup-1) for rivers supporting salmonid fisheries. in fast-flowing aerobic small streams, no(inf2)(sup-) is thought to originate from nitrification, due to the retardation of nitrobacter strains by the presence of free ammonia. multiple regression analyses of no(inf2)(sup-) concentrations against water quality variables of the si ...199716535749
effects of temperature, medium composition, ph, salt and dissolved oxygen on haemolysin and cytotoxin production by aeromonas hydrophila isolated from oyster.the effects of temperature, medium composition, ph, salt content and dissolved oxygen (do) on the production of haemolysin and cytotoxin by one strain of aeromonas hydrophila isolated from oyster were investigated. four media were tested: brain heart infusion broth (bhib), casamino acid-yeast extract broth (cayeb), nutrient broth (nb), and trypticase soy broth (tsb). bhib was the best for toxin production even though the growth rates for aeromonas hydrophila in all of these media were quite simi ...19979506276
isolation and identification of aeromonas species from human stools.one thousand and three diarrhoeal stool samples were processed in our laboratory during the period 1996/1997 for the presence of enteric pathogens especially aeromonas spp., which has emerged as a new agent causing diarrhoea. ampicillin sheep blood agar was found to be the best medium for the isolation of aeromonas spp. from stool specimens. enteric pathogens were found in 200 (20%) stools, of which aeromonas spp. was the second commonest pathogen isolated amounting to 21% of isolates. this stud ...19979510643
rflp-pcr analysis of the aroa gene as a taxonomic tool for the genus aeromonas.the aroa gene has been identified as a target in screening for the presence of most aeromonas species so far described by pcr. synthetic oligonucleotide primers of 24 and 25 nucleotides were used by pcr assay to amplify a sequence of the aroa gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. a 1236-bp dna fragment, representing most of the aroa gene, according to the nucleotide sequence of a. salmonicida, was a ...19979513265
the role of the capsular polysaccharide of aeromonas hydrophila serogroup o:34 in the adherence to and invasion of fish cell lines.the ability of aeromonas hydrophila serogroup o:34 strains grown under different conditions (capsulated and non-capsulated) to adhere to and invade two fish cell lines was compared. the level of adherence was slightly higher when the strains were grown under conditions promoting capsule formation than when the same strains were grown under conditions which did not promote capsule formation. however, the most significant difference among the wild-type strains grown under conditions promoting caps ...19979765847
[application of pyrazinamidase activity for the identification of aeromonas species].70 aeromonas strains isolated from patients with acute diarrheal disease were classified into species by using the camp factor test and the pyrazinamidase activity. it is stressed the high percent of coincidence between both tests, which are easy to make at the clinical microbiology laboratories.19979685960
[comparative study of 4 methods for identifying species of the genus aeromonas].it is given an explanation of the results obtained with the following methods: aerokey ii, aerokey ii + abbot's scheme, api 20 ne, and the biolog system. the study was conducted with 38 strains of aeromonas isolated from children under 5 with acute diarrheal disease (add). the aerokey ii + abbot's scheme proved to be the best identification method.19979685968
enteric bacterial pathogens in stools of residents of urban and rural regions in nigeria: a comparison of patients with and without diarrhoea and controls without diarrhoea.a total of 2,400 stool samples comprising 1,200 from patients with diarrhoea (600 each from urban and rural area) and 1,200 similarly divided controls were obtained from school children and clinic attendants of government and private clinics around three designated study centres of edo, lagos and cross river states, nigeria. these were screened for the prevalence of bacteria that could cause diarrhoea. diarrhoea cases in urban areas had a high prevalence rate for campylobacter spp. (28%), follow ...19979661320
occurrence of gram-negative bacteria in drinking water undergoing softening treatment.a study was carried out on the presence of gram-negative bacteria in the municipal waters of bologna (italy) undergoing softening using domestic ion exchangers with an automatic disinfection mechanism. the softening process was seen to cause a 15 fold increase in 22 degrees c and 36 degrees c heterotrophic plate counts. there was a 30 fold increase in gram-negative bacteria and their number correlated directly with temperature and inversely with active residual chlorine. organic matter had no ef ...19979636986
selenite bioremediation potential of indigenous microorganisms from industrial activated sludge.ten bacterial strains were isolated from the activated sludge waste treatment system (biox) at the exxon refinery in benicia, california. half of these isolates could be grown in minimal medium. when tested for selenite detoxification capability, these five isolates (members of the genera bacillus, pseudomonas, enterobacter and aeromonas), were capable of detoxifying selenite with kinetics similar to those of a well characterized bacillus subtilis strain (168 trp+) studied previously. the seleni ...19979608517
[aeromonas infections]. 19979569900
acute rhabdomyolysis and myonecrosis complicating aeromonas bacteremia in neutropenic patients with hematologic malignancies: report of two cases.infections by aeromonas spp. are a rare cause of systemic infection in normal and immunocompromised hosts. we report the cases of two patients with acute non-lymphoblastic leukemia who developed septic shock by aeromonas species with unusual soft-tissue complications. one patient who was undergoing consolidation chemotherapy developed septic shock by aeromonas hydrophila with rhabdomyolysis and subsequent soft-tissue destruction consistent with myonecrosis. she recovered with combination antibio ...19979499669
meat from dairy cows: possible microbiological hazards and risks.the authors provide an overview of the circumstances associated with culling of dairy cattle in the united states of america (usa) and focus on the possible significant microbiological hazards associated with meat from cull dairy cows. cull dairy cows are an important source of food in the usa, accounting for at least approximately 17% of ground beef. the potential microbiological hazards for foodborne illness from cull dairy cows discussed here include salmonella (with special attention to s. t ...19979501354
the role of seafood in foodborne diseases in the united states of america.in the united states of america, seafood ranked third on the list of products which caused foodborne disease between 1983 and 1992. outbreaks connected with fish vectors were caused by scombroid, ciguatoxin, bacteria and unknown agents; in shellfish, unknown agents, paralytic shellfish poisoning, vibrio spp. and other bacteria, followed by hepatitis a virus, were responsible for the outbreaks. at least ten genera of bacterial pathogens have been implicated in seafood-borne diseases. over the pas ...19979501377
further characterization of renibacterium salmoninarum extracellular products.renibacterium salmoninarum, the agent of bacterial kidney disease in salmonids, releases high concentrations of extracellular protein in tissues of infected fish. the extracellular protein consists almost entirely of a 57-kda protein and derivatives of degradation and aggregation of the same molecule. the 57-kda protein and its derivatives were fractionated into defined ranges of molecular mass. separated fractions continued to produce degradation and aggregation products. one-dimensional electr ...19979480644
[prevalence of aeromonas spp. in surface water].some aeromonas strains are well recognized enteropathogens according to microbiological, clinical, immunological and epidemiological evidence. the main source of infection seems to be untreated water, these microorganisms can be found in virtually all aquatic environments. additionally, some aeromonas, which include enterotoxigenic strains, are capable of rapid growth at 5 degrees c and even of producing toxins. vegetable products irrigated with contaminated water may reach critical aeromonas le ...19979429640
characterization of the bacterial flora isolated from a pilot-scale lagoon processing swine manure.the bacterial flora of an experimental plant that processes liquid swine manure by an aerated compartmented (multistage) lagoon system has been studied. the total flora is characterized by a larger number of oligotrophic bacteria than eutrophic ones. each compartment displays a specific flora, different from the flora in the manure, and consisting of a complex assembly of gram-negative and gram-positive ubiquitous species, such as aeromonas spp. and pseudomonas spp., and specialized species, suc ...19979436310
detection and speciation of bacteria through pcr using universal major cold-shock protein primer oligomers.the detection of bacteria using pcr is a well-established diagnostic technique. however, conventional pcr requires the use of dna primer oligomers that are specific to the target organism and, as a consequence, a sample can only be tested for the presence of that specific target. a significant advantage would be to probe a sample for the presence of any bacteria, followed by identification. to achieve this it is necessary to identify a dna sequence common to all bacteria. here we demonstrate tha ...19979439003
effect of amino acids on the production and activities of cephalosporin c acylase and penicillin v acylase from aeromonas species acy 95.amino acids altered the production and activities of cephalosporin c acylase and penicillin v acylase from aeromonas species acy 95 to a varying degree. dl-tryptophan enhanced the cephalosporin c acylase formation by 222% while suppressed the penicillin v acylase formation by 68%.19979440283
[meropenem: microbiologic perspective].meropenem is a beta-lactamic carbapenem derived from thienamycin and is structurally characterized by the presence of a beta-methyl group in position c1 which confers stability to the molecule versus renal dehydropeptidase 1 (dhp-1), thereby making the coadministration of an enzyme inhibitor unnecessary. its esterochemical configuration of the lateral chain in c2 (dimethyl carbomoilpyrrolidenethium) increases the activity versus gram negative bacteria (enterobacteria and pseudomonas) and moreove ...19979410064
pathogenicity of atypical aeromonas salmonicida in atlantic salmon compared with protease production.the pathogenicity of extracellular products (ecps) from 24 atypical aeromonas salmonicida strains was studied with respect to: lethality in atlantic salmon, pathogenic effect in muscle, haemolytic activity, cytotoxicity in two fish cell lines and proteolytic activities. furthermore, the relationship between lethality of ecps and mortality caused by bacterial challenge was examined. correlation was demonstrated between the pathogenic properties and proteolytic activities of the ecps. cytolytic (g ...19979418019
antimicrobial activity among pseudomonas and related strains of mineral water origin.siderophore production in 382 pseudomonas and related strains of mineral water origin were screened and the antimicrobial activities of 158 of these tested against nine target organisms of health significance. presence of siderophores could be detected in 54.4% and the majority of strains tested (91.2%) inhibited at least one of the nine target strains. staphylococcus, escherichia coli and aeromonas hydrophila were particularly sensitive. addition of iron eliminated the inhibitory activity in 96 ...19979418027
reappraisal of the effect of temperature on the growth kinetics of aeromonas salmonicida.the effect of temperature (1-34 degrees c) on the maximum specific growth rate of aeromonas salmonicida could not be described by the classical growth models; for some strains, two optimal temperatures at 23 degrees c and 30 degrees c were observed, as well as an unexpected increase in the pseudolag time above 27 degrees c. this could be explained by the presence of two subsets, notably s-layer+ and s-layer- sub-populations. the a- cells had higher growth parameters (topt and mu opt) than the a+ ...19979418073
enzyme-linked immunosorbent assay for detection of aeromonas hydrophila serogroup o:19.an enzyme-linked immunosorbent assay has been developed for the detection of aeromonas hydrophila serogroup o:19 isolated from epizootics in eels. the enzyme-linked immunosorbent assay specificity was confirmed after testing a. hydrophila o:19 and non-o:19 strains from different origins, as well as other aeromonas species and other fish pathogens such as vibrio vulnificus biotype 2, v. furnisii, v. damsela, yersinia ruckerii and edwardsiella tarda. the detection limits for a. hydrophila o:19 cel ...19979418247
sequence and expression of an eisenia-fetida-derived cdna clone that encodes the 40-kda fetidin antibacterial protein.fetidins are 40-kda and 45-kda hemolytic and antibacterial glycoproteins present in the coelomic fluid of the earthworm eisenia fetida andrei. by screening a cdna library with a polyclonal antifetidin serum, we have cloned a cdna that encoded the 40-kda fetidin. the clone contains an insert of 1.44 kb encoding a protein of 34 kda, which corresponds to the size of deglycosylated fetidins. the recombinant protein inhibits bacillus megaterium growth. restriction fragment polymorphisms were observed ...19979219536
[bacteremia for aeromonas sobria in a patient with cholecystitis]. 19979221025
molecular and phenotypic methods for the characterization of atypical aeromonas salmonicida.atypical aeromonas salmonicida form a taxonomically diverse group among the psychrophilic a. salmonicida. characteristics of 53 atypical a. salmonicida strains originating from finland, denmark, norway and sweden were studied using 60 phenotypic tests. ribopattern analysis and plasmid profiles were used as genetic methods. the production of brown pigment on the furunculosis agar containing l-tyrosine divided the atypical oxidase-positive strains into two groups: pigment-producing (n = 35) and ac ...19979228690
the role of flagella and motility in the adherence and invasion to fish cell lines by aeromonas hydrophila serogroup o:34 strains.we compared the ability of aeromonas hydrophila wild-type strains of serogroup o:34, non-motile tn5 aflagellar mutants and the same mutants harboring a recombinant cosmid dna from a library of a. hydrophila ah-3 (o:34, wild-type) that allows these mutants to make flagella and to be motile, to adhere and invade two fish cell lines. we found that motility is essential in these strains for adhesion, and also that possession of flagella is essential for the ability to invade the fish cell lines. we ...19979228756
purification and characterization of l-allo-threonine aldolase from aeromonas jandaei dk-39.l-allo-threonine aldolase (l-allo-threonine acetaldehyde-lyase), which exhibited specificity for l-allo-threonine but not for l-threonine, was purified from a cell-free extract of aeromonas jandaei dk-39. the purified enzyme catalyzed the aldol cleavage reaction of l-allo-threonine (k(m) = 1.45 mm, vmax = 45.2 mumol min-1 mg-1). the activity of the enzyme was inhibited by carbonyl reagents, which suggests that pyridoxal-5'-phosphate participates in the enzymatic reaction. the enzyme does not act ...19979228760
secreted enzymes of aeromonas.a hallmark characteristic of species of aeromonas is their ability to secrete a wide variety of enzymes associated with pathogenicity and environmental adaptability. among the most intensively studied are beta-lactamases, lipases, hemolytic enterotoxins, proteases, chitinases, nucleases and amylases. multiple copies of genes encoding each type of enzyme provide additional biological diversity. except for the chitinases, these multiple copies show little evolutionary relatedness at the dna level ...19979228763
computer aided assignment of motile aeromonas strains to genospecies level by standard biochemical tests.a procedure is given for differentiation of motile aeromonas spp. based on nine to 12 biochemical properties it allows an assignment of isolated aeromonas strains first to phenotypes and, in the second step, to hybridization groups (hgs) 1-13 as well as to the genotypically defined species aer. allosaccharophila and aer. encheleia. the computer aided classification is carried out according to the principles of numerical taxonomy. using this differentiation scheme 23 aeromonas strains, which were ...19979230673
molecular and phenotypic features of aeromonads isolated from snails (helix aspersa) affected with a new summer disease.forty-four aeromonads were isolated during the summer of 1994 from snails (helix aspersa) affected or not by a newly described disease. snta 1 rrna gene restriction patterns (ribotyping) and nine selected conventional phenotypic tests were performed to retrospectively determine relationships among these isolates regarding the disease. results from ribotyping were found highly discriminant in strain differentiation and suggest the possible existence of a group of aeromonas associated with the dis ...19979230675
structurally defined epitopes of haemophilus ducreyi lipooligosaccharides recognized by monoclonal antibodies.by use of enzyme-linked immunosorbent assay and immunoblotting techniques, the migration patterns and binding epitopes of lipooligosaccharides (los) from 10 haemophilus ducreyi strains were investigated with two monoclonal antibodies (mabs), mahd6 and mahd7, raised against los from h. ducreyi itm 2665. closely related los, with defined structures, from haemophilus influenzae, bordetella pertussis, aeromonas spp., and synthetic glycoproteins were also included in the analyses. the mabs bound to c ...19979234768
continuous spectrophotometric assay of peptide deformylase.a continuous assay for peptide deformylase has been developed using a formylated dipeptide, formyl-met-leu-p-nitroanilide, as substrate. removal of the formyl group by a peptide deformylase renders the dipeptide product, which contains a free nh2 terminus, a substrate for an aminopeptidase from aeromonas proteolytica. sequential hydrolysis of the dipeptide by the aminopeptidase releases a p-nitroaniline, which is monitored spectrophotometrically at 405 nm. this assay was applied to determine the ...19979234895
rapid purification, partial characterization, and antimicrobial spectrum of the bacteriocin, pediocin acm, from pediococcus acidilactici m.the bacteriocin from pediococcus acidilactici m, designated as pediocin acm, was rapidly purified to homogeneity by the ph mediated cell adsorption-desorption method and semi-preparative reversed-phase hplc. the purification yield was 40.4% and the specific activity was increased by 2450-fold. it gave a single band and a single peak on sds-page and hplc analysis, respectively. when subjected to electrospray lc-ms analysis, the protein was found to be highly pure and the molecular weight was dete ...19979237116
evaluation of the synergistic haemolysis (camp-like) test in the identification of motile, mesophilic aeromonas species.usefulness of the synergistic haemolysis (camp-like) test in the identification of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae was evaluated, using beta-toxin producing staphylococcus aureus atcc 25923 and alpha-toxin producing clostridium perfringens atcc 13124 as sphingomyelinase producing indicator strains. the tests were done on columbia blood agar base supplemented with 5% sheep blood (cba; difco laboratories, detroit, mi, usa). when cultured aerobically, 77% of a. hydrophi ...19979241799
pyomyositis of the anterior tibial compartment.five oncology patients developed bacterial pyomyositis involving the anterior tibial compartment and resulting in compartment syndrome with ischemia and abnormalities of neuromuscular function. all patients were neutropenic and thrombocytopenic, and four were receiving or had recently received cancer chemotherapy. three infections were due to gram-negative bacilli and two to staphylococcus aureus. appropriate antimicrobial therapy and surgical drainage in four patients resulted in the resolution ...19979243048
cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of aeromonas caviae.a 5.0-kbp ecorv-ecori restriction fragment was cloned and analyzed from genomic dna of aeromonas caviae, a bacterium producing a copolyester of (r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyhexanoate (3hhx) [p(3hb-co-3hhx)] from alkanoic acids or oils. the nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (pha) synthase gene (phac(ac) [i.e., the phac gene from a. caviae]) together with four open reading frames (orf1, -3, -4, and -5) and one putative promoter region. t ...19979244271
spectroscopically distinct cobalt(ii) sites in heterodimetallic forms of the aminopeptidase from aeromonas proteolytica: characterization of substrate binding.the co(ii)zn(ii)- and zn(ii)co(ii)-substituted derivatives of the aminopeptidase from aeromonas proteolytica (aap) were probed by epr spectroscopy. epr spectra of the high-spin s = 3/2 co(ii) ions in [cozn(aap)] and [znco(aap)] indicated that each metal binding site provides a spectroscopically distinct signature. for [cozn(aap)], subtraction of epr spectra recorded at ph 7.5 and 10 revealed that two species were present and that the relative contributions to each of the experimental spectra wer ...19979245416
mode of primary binding to target membranes and pore formation induced by vibrio cholerae cytolysin (hemolysin).vibrio cholerae cytolysin (vcc) is produced by many non-choleratoxigenic strains of v. cholerae, and possibly represents a relevant pathogenicity determinant of these bacteria. the protein is secreted as a pro-toxin that is proteolytically cleaved to yield the active toxin with a molecular mass of approximately 63 kda. we here describe a simple procedure for preparative isolation of mature vcc from bacterial culture supernatants, and present information on its mode of binding and pore formation ...19979249028
cloning and expression of the cdna encoding prolyl oligopeptidase (prolyl endopeptidase) from bovine brain.prolyl oligopeptidase (ec 3.4.21.26, prolyl endopeptidase) cdna from bovine brain was cloned by pcr, and the amplified fragment was used as a probe to screen the cdna library from bovine brain. the obtained clone contained a 2.7 kb dna fragment with an open reading frame of 2130 nucleotides, and encoded a protein of 710 amino acids with a deduced molecular weight of 80640 da. the deduced amino acid sequence is 95, 94, 51 and 48% homologous to those of human t-cell, porcine brain, aeromonas hydro ...19979353562
cloning and sequence analysis of the gene (epra1) encoding an extracellular protease from aeromonas hydrophila.a gene (epra1) encoding the extracellular protease of aeromonas hydrophila ah1 has been cloned and sequenced. nucleotide sequence analysis of epra1 predicted a single open reading frame (orf) of 1038 bp encoding a 346 amino acid (aa) polypeptide, with a potential 21-aa signal peptide. when the epra1 gene was expressed in minicells, one major band of approx. 37 kda was identified, while protease activity staining experiments identified a caseinolytic band of approx. 29 kda determined by sds-page ...19979358060
expression and characterization of the recombinant gene encoding chitinase from aeromonas caviae.the gene encoding a chitinase from aeromonas caviae was cloned by pcr techniques. its recombinant gene expression was performed using pet20b(+) in escherichia coli bl21 (de3). the recombinant chitinase with the extra 33 and 13 amino acids in its n- and c-termini, respectively, was purified to near homogeneity using his-tag affinity chromatography. the recombinant chitinase was found to be present in both the culture medium and the cytoplasm. a single protein band on the native polyacrylamide gel ...19979358657
development and application of monoclonal antibodies for in situ detection of indigenous bacterial strains in aquatic ecosystems.strain-specific monoclonal antibodies (mabs) were developed for three different bacterial isolates obtained from a freshwater environment (lake plusssee) in the spring of 1990. the three isolates, which were identified by molecular methods, were as follows: cytophaga johnsonae px62, comamonas acidovorans px54, and aeromonas hydrophila pu7718. these strains represented three species that were detected in high abundance during a set of mesocosm experiments in lake plusssee by the direct analysis o ...19979361440
aeromonas sobria-associated left-sided segmental colitis.aeromonas species, once thought pathogenic only in immunocompromised hosts, have more recently been found to be associated with many different infections in previously healthy individuals. the most common site of these infections is the gastrointestinal tract. we describe a 67-yr-old woman who presented with abdominal pain and bloody diarrhea and was diagnosed with left-sided, segmental colitis due to aeromonas sobria, which was proven by stool culture. extensive work-up for ischemic colitis was ...19979362202
incidence and clinical symptoms of aeromonas-associated travellers' diarrhoea in tokyo.in a survey examining the causes of travellers' diarrhoea treated in tokyo between july 1986 and december 1995, aeromonas species were isolated from 1265 (5.5%) of 23,215 travellers returning from developing countries. aeromonas species were the fourth most frequent enteropathogen isolated, following enterotoxigenic e. coli (8.5%), salmonella spp. (7.6%) and plesiomonas shigelloides (5.6%). aeromonas species were found in 1191 (5.6%) of 21,257 patients with diarrhoea and in 74 (3.8%) of 1958 hea ...19979363009
influence of iron, growth temperature and plasmids on siderophore production in aeromonas hydrophila.aeromonas hydrophila strains obtained from diarrhoeal samples of human patients (19 isolates) and freshwater ponds (11 isolates) were analysed for siderophore production. both clinical and environmental isolates showed significantly increased siderophore production under iron-limiting conditions both at 28 degrees c and at 37 degrees c. clinical isolates consistently produced higher levels of siderophores than did the environmental isolates. the role of plasmids in moderating siderophore product ...19979364139
determination of the amino acid sequence of the plant cytolysin enterolobin.the cytolytic seed protein enterolobin from seeds of enterolobium contortisiliquum was purified by using fplc on a mono q column giving a single peak in capillary electrophoresis. the complete amino acid sequence of the plant cytolysin was determined by an automated method, yielding a molecular mass of 54,806 da. databank searches and sequence alignment demonstrated a high degree of sequence identity and similarity between enterolobin and bacterial aerolysins from aeromonas hydrophila and a. sob ...19979367525
general secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in vibrio cholerae.the general secretion pathway (gsp) of vibrio cholerae is required for secretion of proteins including chitinase, enterotoxin, and protease through the outer membrane. in this study, we report the cloning and sequencing of a dna fragment from v. cholerae, containing 12 open reading frames, epsc to -n, which are similar to gsp genes of aeromonas, erwinia, klebsiella, pseudomonas, and xanthomonas spp. in addition to the two previously described genes, epse and epsm (m. sandkvist, v. morales, and m ...19979371445
[transient bacteremia caused by aeromonas jandaei]. 19979376410
[ascitic fluid infection and enteritis by aeromonas hydrophila]. 19979379726
[the prevalence of aeromonas spp. in the diarrheal feces of children under the age of 5 years in the city of goiânia, goiás in the 1995-1996 biennium].from 1995 through 1996, 163 fecal specimens of children aged under 5 years were analysed, 91 being from diarrhea feces and 72 without diarrhea. the material was collected in transport medium and submitted to the enrichment procedure at 4 degrees c for 7 days. for the primary isolation starch ampicillin agar was used and incubated at 35 degrees c for 18 to 24 hours. twenty (20.9%) from the following specimens were isolated: aeromonas (a.) caviae (7.7%), a. salmonicida salmonicida (6.6%), a. sobri ...19979380898
[piperacillin/tazobactam in the treatment of abdominal sepsis in patients with peritonitis].piperacillin/tazobactam (p/t) was used in the treatment of 40 patients at the age of 22 to 64 years with intraabdominal infection (peritonitis, abscesses). p/t was administered as 30-minute intravenous infusions 4 or 3 times a day for 3 to 13 days. the recovery and improvement were stated in 80 and 10 per cent of the cases respectively. in 10 per cent of the patients the treatment failed because of surgical complications or insufficient surgical intervention or because of some other systemic dis ...19979124988
pathogenic analysis of aeromonas hydrophila septicemia.aeromonas hydrophila has emerged as a potential pathogen in the immunocompromised host. various aeromonal infections, including septicemia, have also been reported in apparently healthy individuals. for years, researchers have disagreed over the epidemiologic roles of aeromonads in gastroenteritis. isolation rates of aeromonads by stool culture among patients with gastroenteritis are not consistently high. carriers of this bacterium also exist. the septicemic course is, however, often fulminant ...19979210970
a monoclonal antibody reacts with maltose-binding protein of escherichia coli and related enteric bacteria.maltose-binding protein (mbp) encoded by male is essential for the energy-dependent translocation of maltose through the cytoplasmic membrane of bacteria. its property of specific binding to maltose has been used in constructing fusion proteins for easy affinity purification. a monoclonal antibody named mab sc1d7 was produced against escherichia coli mbp. this mab also bound to mbp-containing recombinant proteins in both western blotting and immunoprecipitation analysis. as a result, this mab ca ...19979212834
bacterial pathogens associated with diarrhoea on the island of crete.stool specimens from 3,600 diarrhoeal patients from the island of crete, greece, were examined for bacterial pathogens, during a three-year period (1992-1994). one or more pathogens were identified in 826 patients (22.9%), more often from children. salmonella spp. were the most frequently isolated organisms in 13.6% of the patients, followed by campylobacter in 4.7%, and enteropathogenic escherichia coli (epec) in 3.9%. yersinia enterocolitica was found in 0.7%, shigella spp. in 0.7% and aeromon ...19979384274
species, biotype and serogroup of campylobacter spp. isolated from children with diarrhoea over a ten-year period.from 1981 to 1990, stool samples from 6403 gastroenteritis cases were examined for the presence of campylobacters as well as salmonella, shigella, aeromonas species and yersinia enterocolitica. the percentages of isolation were the following: campylobacters 10.8 (86.1% of isolates were c. jejuni and 13.9% were c. coli), salmonella spp. 8.4, aeromonas spp 1.4, yersinia enterocolitica 0.3. shigella spp. were isolated only occasionally. predominant biotypes of campylobacters were c. jejuni i (69.5% ...19979385599
aeromonas sobria endophthalmitis.aeromonas sobria causes a rare gram-negative bacterial water-borne infection. it has been found in waters of north queensland and south-east asia. of all aeromonas species, a. sobria is the most virulent and invasive and has been reported to cause soft tissue infection and corneal ulcer.19979395834
inhibition of the metallo-beta-lactamase produced from serratia marcescens by thiol compounds.low molecular weight thiol compounds have been found to be strong inhibitors of metallo-beta-lactamase (imp-1) produced by serratia marcescens tn9106, which was expressed by echerichia coli jm109 cells. mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited imp-1 with ki of 0.23 and 0.19 microm, respectively. 2-mercaptoethanol reversibly inhibited imp-1 but did not show simple competitive inhibition.19979401719
antimicrobial activity of 12 broad-spectrum agents tested against 270 nosocomial blood stream infection isolates caused by non-enteric gram-negative bacilli: occurrence of resistance, molecular epidemiology, and screening for metallo-enzymes.a total of 270 recent nosocomial blood stream isolates of non-enterobacteriaceae gram-negative bacilli representing nearly 50 u.s. medical centers were characterized. the numbers of isolates of individual organisms were: pseudomonas aeruginosa (n = 204), acinetobacter spp. (n = 48), and stenotrophomonas maltophilia (n = 18). mics were determined using the broth microdilution susceptibility method with 12 antimicrobial agents: piperacillin, piperacillin/tazobactam, ceftriaxone, ceftazidime, cefep ...19979401811
virulence factors-pathogenicity relationships for aeromonas species from clinical and food isolates.the presence of virulence factors in 96 aeromonas strains isolated from food and clinical samples was studied. neither cytotoxic activity and hydrophobicity, not the presence of pili or an extra surface layer made it possible to establish differences between food and clinical strains. statistical studies showed that cytotoxin production was associated with a positive voges-proskauer reaction, inability to ferment arabinose and a positive lysine decarboxylation. therefore, when comparing cytotoxi ...19979449784
[detection of the thermostable lipase gene (lipah) in clinical isolates of aeromonas hydrophila belonging to the major o serogroups o11, o16 and o34].on the basis of dna hybridization data and phenotypes, most pathogenic strains of aeromonas hydrophila are grouped into hybridization group 1 (hg1). these pathogenic strains secret the theromostable lipase, and it's gene (lipah) has been cloned and sequenced. the present study was performed to identify the pathogenic strains of a. hydrophila by the polymerase chain reaction (pcr) technique to detect the lipah. synthetic oligonucleotide primers (lipah-ns-1 and -na-1) were used in the pcr. the pcr ...19979455059
the suppressive excretory-secretory product of trichobilharzia ocellata: a possible factor for determining compatibility in parasite-host interactions.factors which may determine trematode-snail interactions were assessed in the present study. compatibility was examined using a bacterial clearance assay to detect the modulatory effects of both compatible and incompatible trematode infections on the activity of haemocytes from lymnaea stagnalis, during the early stages of infection. exposure to and injection with trichobilharzia ocellata, a compatible trematode, or the incompatible schistosoma mansoni, resulted in modulation of haemocyte activi ...199710190175
diversity, persistence, and virulence of aeromonas strains isolated from drinking water distribution systems in sweden.the aeromonas populations in 13 swedish drinking water distribution systems, representing different treatments, were investigated. from each system, water samples were collected four times during the period from may to september 1994 from raw water and water after treatment and at two to five sites within the distribution system. in total, 220 water samples were collected. from samples containing presumptive aeromonas, up to 32 colonies were analyzed by the pheneplate aeromonas (php-ae) system, ...199711799982
isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine.bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ...199710932719
polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria.polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ...199712501353
acute phase proteins in salmonids: evolutionary analyses and acute phase response.inflammation induces dramatic changes in the biosynthetic profile of the liver, leading to increased serum concentrations of positive acute phase (ap) proteins and decreased concentrations of negative ap proteins. serum amyloid a (saa) and the pentraxins c-reactive protein (crp) and serum amyloid p component (sap) are major ap proteins: their serum levels can rise by 1000-fold, indicating that they play a critical role in defense and/or the restoration of homeostasis. we have cloned saa and a sa ...19978977214
inhibition of metallo-beta-lactamases by a series of mercaptoacetic acid thiol ester derivatives.a series of mercaptoacetic acid thiol esters have been identified as metallo-beta-lactamase inhibitors. electrospray mass spectrometry (esms) has shown that irreversible inhibition of the bacillus cereus ii metallo-beta-lactamase by sb214751, sb214752, and sb213079 was concomitant with a 90-da increase in mass of the enzyme. tryptic digestion of the b. cereus ii inhibited with sb214751 illustrated that the peptide fragment, containing the only cysteine of the enzyme, had undergone a mass increme ...19978980769
characterization of aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in bangladesh.aeromonas isolates from patients with diarrhea in bangladesh (n = 69), from healthy controls (n = 11), and from surface water (n = 40) were analyzed with respect to their hybridization groups (hgs) by the aid of fatty acid methyl ester (fame) characterization and dna fingerprinting by aflp, biochemical phenotypes (phe-neplate [php] types), and the production of hemolysin and cytotoxin. the aim of the investigation was to find out whether certain strains carrying virulence factors predominated am ...19979003598
production of monoclonal antibodies to the non-membrane-damaging cytotoxin (nmdcy) purified from vibrio cholerae o26 and distribution of nmdcy among strains of vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay.the distribution of a newly described secretogenic non-membrane-damaging cytotoxin (nmdcy) among strains of vibrio cholerae and other enteric bacteria was determined. to accomplish this, monoclonal antibodies against nmdcy were prepared and a sandwich monoclonal-polyclonal enzyme-linked immunosorbent assay (elisa) was developed. by the sandwich elisa, it was determined that 55.6% of the 412 strains of v. cholerae examined produced nmdcy at varying concentrations while 76, 37.9, and 15.6% of the ...19979009346
an enzyme-linked immunosorbent assay for detection of vibrio vulnificus biotype 2: development and field studies.vibrio vulnificus biotype 2 is a primary eel pathogen which constitutes a lipopolysaccharide (lps)-based homogeneous o serogroup within the species. in the present work, we have developed an enzyme-linked immunosorbent assay (elisa) based on the specificity of lps for the detection of this pathogen. the elisa specificity was confirmed after testing 36 biotype 2 strains from laboratory cultures and environmental samples, 31 clinical and environmental biotype 1 isolates, and several strains of vib ...19979023934
isolation and characterization of chitinase from a flake-chitin degrading marine bacterium, aeromonas hydrophila h-2330.a flake-chitin degrading marine bacterium was isolated and identified as aeromonas hydrophila. this strain secreted five chitinases and an beta-n-acetylglucosaminidase. the main chitinase (chi-a) was purified and characterized. the optimum ph of chi-a was 5-8, and the activity was inhibited by hg2+ and fe3+. chi-a was different from chitinases of other aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. the amino-terminal amino acid sequence showed e ...19979028048
aeromonas species in fish, fish-eggs, shrimp and freshwater.aeromonas spp. are common contaminants of fish and seafood. they also are ubiquitous in the water environment. aeromonas spp. were identified in 27 (93%) of 29 fish, in 17 (100%) fish-egg, in two (16%) of 12 shrimp samples and in 23 (100%) freshwater samples. in total, 117 aeromonas strains were isolated from 69 positive samples, several samples having had two or three aeromonas species. included in this were also 26 mesophilic aeromonas strains isolated in association with the study on fish dis ...19979029253
carbapenem-hydrolyzing beta-lactamases. 19979021171
aeromonas hydrophila soft-tissue infection: a report of two cases. 19979043548
streptomyces griseus aminopeptidase: x-ray crystallographic structure at 1.75 a resolution.the x-ray crystal structure of the enzyme streptomyces griseus aminopeptidase (sgap) has been determined in its double zinc form to 1.75 a resolution, in its apo-enzyme from (zinc removed) to 2.1 a resolution, and as a mercury replaced derivative to 2.1 a resolution. the structure solution was achieved by single isomorphous replacement with phasing from anomalous scattering (siras), followed by density modification with histogram matching. the protein consists of a central beta-sheet made up of ...19979048953
a 4-year study of the diversity and persistence of coliforms and aeromonas in the water of a swedish drinking water well.coliforms and aeromonas, isolated over a sampling period of 4 years from a swedish drinking water well, were analysed for their phenotypical diversity and for their ability to persist in the well water. from each of the 40 water samples collected from the well, 32 bacterial isolates were subjected to typing by the pheneplate (php) biochemical fingerprinting system. strains able to persist in the well water were further characterized using the api 20e system, gas-liquid chromatographic cellular f ...19979057291
antibiotic-induced antimicrobial resistance in aeromonas spp. 19979059443
expression of the asba1, oxa-12, and asbm1 beta-lactamases in aeromonas jandaei aer 14 is coordinated by a two-component regulon.aeromonas jandaei aer 14 (formerly aeromonas sobria aer 14) expresses three inducible beta-lactamases, asba1, oxa-12 (asbb1), and asbm1. mutant strains that constitutively overexpress all three enzyme simultaneously, suggesting that they share a common regulatory pathway, have been isolated. detectable expression of the cloned genes of asba1 and oxa-12 in some escherichia coli k-12 laboratory strains is achieved only in the presence of a blp mutation. these mutations map to the cre operon at 0 m ...19979068648
crystal structure of carboxypeptidase g2, a bacterial enzyme with applications in cancer therapy.carboxypeptidase g enzymes hydrolyze the c-terminal glutamate moiety from folic acid and its analogues, such as methotrexate. the enzyme studied here, carboxypeptidase g2 (cpg2), is a dimeric zinc-dependent exopeptidase produced by pseudomonas sp. strain rs-16. cpg2 has applications in cancer therapy: following its administration as an immunoconjugate, in which cpg2 is linked to an antibody to a tumour-specific antigen, it can enzymatically convert subsequently administered inactive prodrugs to ...19979083113
molecular analysis and expression of the extracellular lipase of aeromonas hydrophila mcc-2.the structural gene encoding the extracellular lipase of aeromonas hydrophila mcc-2 was cloned and found to be expressed in escherichia coli using its own promoter. when the cloned gene (lip) was expressed in e. coli minicells, an 80 kda protein was identified. subcellular fractionation of e. coli carrying the lip gene indicated that the lip protein was mainly associated with the membrane fraction. nucleotide sequence analysis revealed that the gene is 2253 bp long, coding for a 79-9 kda protein ...19979084164
sensitivity of aeromonas hydrophila carbapenemase to delta3-cephems: comparative study with other metallo-beta-lactamases.ceftriaxone and ceftriaxone s-oxide behaved as inactivators against the metallo-beta-lactamase of aeromonas hydrophila ae036 and as substrates for the zinc beta-lactamase produced by bacillus cereus (569/h/9) and stenotrophomonas maltophilia ula 511. moreover, ro 09-1428, a catechol-cephalosporin, was not recognized by the a. hydrophila enzyme. panipenem, cephalosporin c, cephalosporin c-gamma-lactone, and loracarbef were substrates for the three studied beta-lactamases.19979087509
[severe acute gastroenteritis due to aeromonas in a patient colectomized for crohn's disease].we report a case of severe acute diarrhoea produced by aeromonas sobria, a specimen with controversial human pathogenicity. a 26-year-old man with crohn's disease previously colectomized was admitted with a cholera-like clinical presentation and renal failure. aeromonas sp. can be isolated in both children and adults and in healthy people, a. hydrophila being most often detected. severe infections due to other aeromonas sp., usually a. sobria and a. caviae, have been rarely described. most patie ...19979090982
transcription analysis of the staphylococcus aureus gene encoding penicillin-binding protein 4.the high level of cross-linking found in staphylococcus aureus peptidoglycan is dependent on the low-molecular-weight penicillin-binding protein pbp4. recently, the pbp4 gene, pbpd, was cloned and shown to be adjacent to and divergently transcribed relative to the putative abc-type transporter gene, abca. disruption of abca (in strain kb400) was previously shown to result in heightened resistance to several antibiotics known to interact with pbp4, suggesting that the regulation of pbpd is affect ...19979098064
mechanistic studies on the aminopeptidase from aeromonas proteolytica: a two-metal ion mechanism for peptide hydrolysis.the aminopeptidase from aeromonas proteolytica (aap) is uncompetitively inhibited by fluoride ion at ph 8.0 with an inhibition constant (ki) of 30 mm. thus, fluoride inactivates aap only after substrate binding, and only a single fluoride ion binds to aap. on the other hand, chloride ion does not inhibit aap up to concentrations of 2 m at ph 8.0. the ph dependence of fluoride inhibition of aap was measured over the ph range 6.0-9.5. between ph values of 6.0 and 9.0, fluoride ion acts as a pure u ...19979100023
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