TitleAbstractYear(sorted ascending)
production of extracellular proteases and amylases by some acidophilic and alkalophilic bacteria.twelve bacteria were isolated from two effluent sources of shaw-wallace gelatins, jabalpur. six bacteria from dicalcium phosphate plant effluent (ph-5) and six from main drain of the factory (ph-10) were isolated. two facultatively acidophilic and two facultatively alkalophilic bacteria were selected and tentatively identified as plesiomonas shigelloides, aeromonas hydrophilla, klebsiella pneumoniae and staphylococcus saprophyticus respectively. acidic amylases were produced in higher amounts on ...19968781033
expression of the chitinase iii gene of aeromonas sp. no. 10s-24 in escherichia coli.the chitinase iii gene of aeromonas sp. no. 10s-24 was expressed in escherichia coli. production of chitinase iii by e. coli was 3-fold higher than that of chitinases in the culture supernatant of aeromonas sp. the enzyme from e. coli was purified and characterized. the molecular weight of the chitinase iii from e. coli was estimated by sds-polyacrylamide gel electrophoresis (sds-page) to be 55,000. this agreed with the calculated molecular weight of the mature chitinase iii (54,111). however, i ...19968782417
evaluation of chromagar orientation for differentiation and presumptive identification of gram-negative bacilli and enterococcus species.a new chromogenic plate medium, chromagar orientation, was evaluated for use in the differentiation and presumptive identification of gram-negative bacilli and enterococcus species by a multipoint inoculation (replicator) technique. in this study, 1,404 gram-negative bacilli and 74 enterococcal isolates were tested on chromagar orientation. six control american type culture collection strains were also included with the testing to ensure quality control of the media. of the escherichia coli isol ...19968784591
differential susceptibility of aeromonads and coliforms to cefsulodin.cefsulodin was evaluated as a potential selective agent for aeromonads. resistance of aeromonas and coliform isolates was determined by using a standard disk diffusion technique. a total of 119 aeromonas and 78 coliform strains were isolated. for 102 of 130 [corrected] aeromonas isolates (environmental and reference strains), the mic of cefsulodin was < 8 micrograms/ml. results of mic tests by the agar dilution method showed that a concentration of cefsulodin of 10 micrograms/ml or less inhibite ...19968787387
optical single-channel analysis of the aerolysin pore in erythrocyte membranes.scanning microphotolysis (scamp), a recently developed photobleaching technique, was used to analyze the transport of two small organic anions and one inorganic cation through single pores formed in human erythrocyte membranes by the channel-forming toxin aerolysin secreted by aeromonas species. the transport rate constants of erythrocyte ghosts carrying a single aerolysin pore were determined to be (1.83 +/- 0.43) x 10(-3) s-1 for lucifer yellow, (0.33 +/- 0.10) x 10(-3) s-1 for carboxyfluoresc ...19968789089
prevention of suicide phenomenon in aeromonads.clinical isolates of aeromonas (13 aeromonas caviae), 8 aeromonas hydrophila, 3 aeromonas spp., and 2 aeromonas media recovered from diarrheal feces of children were submitted to the suicide phenomenon test and investigated at intervals of 24 h for up to 120 h. the same isolates were also stimulated by repeated passages in broth for ten days before the test. after the bacterial stimulus, a decreases in the number of aeromonads with suicidal capacity was observed. the suicide phenomenon was expre ...19968793406
inactivation of aeromonas hydrophila by fe(ii)-related-radical generation in oxidizing groundwaters.the survival of aeromonas hydrophila awwx1 in filter-sterilized phreatic groundwaters was studied by using viable counts. aeromonas counts rapidly decreased 2 to 3 log units in oxidizing raw groundwaters from snellegem and beernem, belgium (snellegem-raw and beernem-raw, respectively), containing high concentrations of fe2+ (460 to 1,070 microm). the rapid decline in viable counts of aeromonas cells in the oxidizing raw groundwater of snellegem was prevented by the addition of an fe2+ chelator ( ...19968795217
aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (ht-29).aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%). hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and w ...19968795237
differential media for quantitative recovery of waterborne aeromonas hydrophila.because of the ubiquity of aeromonas spp., their prevalence in drinking water, and the increasing number of reports on aeromonas sp.-related infections, a standard method for routine and quantitative recovery had to be defined. on the basis of a survey of 10 media for recovery analysis and subsequent differentiation assays in mixed cultures, we conclude that ampicillin-dextrin agar performed the best for the recovery of aeromonas spp. in drinking water and the differentiation by simple criteria ...19968795251
fulminant pneumonia and sepsis due to aeromonas hydrophila in an alcohol abuser.a 69-year-old alcoholic man with pneumonia and sepsis due to aeromonas hydrophila is presented. he died of suffocation by a copious amount of hemoptysis six hours after his first symptoms of abdominal pain, diarrhea and dyspnea. aeromonas hydrophila was isolated from blood and bronchial secretion. a fulminant form of pneumonia could develop in patients with predisposing underlying conditions such as alcoholism with chronic hepatitis and diabetes mellitus. aeromonas hydrophila pneumonia may be ch ...19968797058
multiple regulation of carp (cyprinus carpio l.) macrophages and neutrophilic granulocytes by serum factors: influence of infection with atypical aeromonas salmonicida.normal carp serum contains inhibitory and stimulatory factors for macrophage and neutrophilic granulocyte respiratory burst activity. as stimulatory factors were only effective in combination with phorbol myristate actetate (pma) activation, it is concluded that they are probably linked to protein kinase c activation. both the stimulatory and inhibitory factors are heat stable. macrophage- and neutrophilic granulocyte-enriched cell fractions from the pronephros of carp had high respiratory burst ...19968797288
characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (cyprinus carpio).a detergent-solubilized fraction of skin mucus of carp (cyprinus carpio) induced ion channels after reconstitution into planar lipid bilayers. a differential extraction using a non-ionic detergent followed by electrophoretic separation led to the isolation of two hydrophobic 31-kda and 27-kda proteins. in contrast to the 27-kda protein, which was glycosylated, the 31-kda did not bind to concanavalin a. the reconstitution of these proteins into a planar lipid bilayer restored the ionophore behavi ...19968797847
ecological relationship between aeromonas and vibrio spp. and planktonic copepods in the coastal marine environment in southern italy.the colonisation of planktonic copepod integument by bacteria belonging to the family of vibrionaceae is a well described phenomenon. in this study, besides reporting on the occurrence of vibrionaceae and other enteropathogens, we further report on the bacterial attachment to the estuarine copepod acartia margalefi in a faecal polluted coastal lagoon near naples, southern italy. in addition, we also performed a laboratory experiment to study the ability of 7 bacterial strains (vibrio cholerae no ...19968800550
survival of cells and dna of aeromonas salmonicida released into aquatic microcosms.the survival of the bacterial fish pathogen aeromonas salmonicida, and persistence of its dna, were monitored in aquatic microcosms using selective culture and most probable number pcr. bacterial cells and naked dna were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. two different types of surface sediment were used. one was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. inoculated cells and n ...19968810058
the role of the capsular polysaccharide of aeromonas salmonicida in the adherence and invasion of fish cell lines.the ability of several aeromonas salmonicida strains grown under different conditions (capsulated and non-capsulated) to adhere to and invade two fish cell lines was compared. the level of adherence was slightly higher when the strains were grown under conditions promoting capsule formation than when the same strains were grown under conditions which did not promote capsule formation. however, the most significant difference among the wild-type strains grown under conditions promoting capsule fo ...19968810501
differentiation of thermolysins and serralysins by monoclonal antibodies.two monoclonal antibodies (mabs) to a 36-kda extracellular metalloprotease (pscp) from burkholderia (pseudomonas) cepacia were found to react with thermolysin, pseudomonas aeruginosa elastase, alkaline protease (apr) and lasa, serratia marcescens protease (smp), aeromonas hydrophila protease (ahp), and both the lethal factor (lf) and protective antigen (pa) of bacillus anthracis on immunoblots. the mabs were capable of neutralising the proteolytic activity of thermolysin, p. aeruginosa elastase ...19968810950
association of aeromonas hydrophila and vibrio alginolyticus with larval mortalities of scallop (argopecten purpuratus)a bacteriological study was carried out in a hatchery of argopecten purpuratus located in northern chile which had been affected by severe larval mortalities. the phenotypic characterization of the bacterial strains revealed that vibrio alginolyticus was the predominant species isolated in the majority of samples taken from the different units of the hatchery (microalgae, swimming larvae, seawater of larval culture tanks, and a reservoir tank of 50-μm filtered seawater). however, the bacterial p ...19968812601
further studies on biochemical characteristics and serologic properties of the genus aeromonas.we characterized a collection of 268 aeromonas isolates from diverse sources (clinical, animal, and environmental sources) for their species and serogroup designations. overall, 97% of these strains could be identified to the genomospecies level by using an expanded battery of biochemical tests. members of the aeromonas hydrophila complex (a. hydrophila, hg2, and a. salmonicida), a group that has previously been difficult to separate biochemically, could easily be distinguished from one another ...19968818884
cloning of an aeromonas hydrophila type iv pilus biogenesis gene cluster: complementation of pilus assembly functions and characterization of a type iv leader peptidase/n-methyltransferase required for extracellular protein secretion.aeromonas hydrophila secretes several extracellular proteins that are associated with virulence including an enterotoxin, a protease, and the hole-forming toxin, aerolysin. these degradative enzymes and toxins are exported by a conserved pathway found in many gram-negative bacteria. in pseudomonas aeruginosa this export pathway and type iv pilus biogenesis are dependent on the product of the pild gene. pild is a bifunctional enzyme that processes components of the extracellular secretory pathway ...19968820654
[bacteriological studies of travellar's diarrhoea. 5) analysis of enteropathogenic bacteria at osaka airport quarantine station from january 1992 through september 3rd, 1994].during the last 2 years and 8 months before the closure of osaka airport quarantine station (from jan. 1992 to sep. 3, 1994), a total of 7,421,909 overseas travellers were quarantined. 15,919 reported themselves of suffering from diarrhoea. bacteriological examination of a total of 6,031 individuals' stools were performed. 1) various enteropathogenic bacteria were isolated from 31.2% of the stools examined. isolated species were as follows: plesiomonas shigelloides, 1,127 cases (59.9%); vibrio p ...19968822051
[in vitro antibacterial activity of pazufloxacin (pzfx) against clinical isolates from infectious enteritis].antibacterial activity of pazufloxacin (pzfx) against 65 strains of shigella spp., 13 strains of salmonella spp., 7 strains of escherichia coli, 4 strains of vibrio parahaemolyticus, 4 strains of v. cholerae o1, 2 strains of aeromonas spp., 4 strains of plesionomas shigelloides and 3 strains of v. cholerae non-o1 isolated from patients of infectious enteritis and carriers was compared with that of ofloxacin (oflx), ciprofloxacin (cpfx), tosufloxacin (tflx) and nalidixic acid (na). the mic90 of p ...19968822053
synergistic iron reduction and citrate dissimilation by shewanella alga and aeromonas veroniitwo bacterial isolates from great bay estuary, new hampshire, in co-culture carried out anaerobic dissimilation of citric acid with fe(iii) as the terminal electron acceptor. neither isolate oxidized citrate with fe(iii) anaerobically in axenic culture. the fe(iii) reducer, shewanella alga strain bry, did not grow anaerobically with citrate as an energy source. the citrate utilizer, aeromonas veronii, did not reduce iron axenically with a variety of electron donors including citrate. the onset o ...19968824150
aerolysin--the ins and outs of a model channel-forming toxin.aerolysin is one of a large group of bacterial proteins that can kill target cells by forming discrete channels in their plasma membranes. the toxin has many properties in common with the porins of the gram-negative bacterial outer membrane, including an extensive amount of beta-structure, a high proportion of hydrophilic amino acid side-chains and no hydrophobic stretches in the primary structure. it also oligomerizes to produce an insertion-competent state. aerolysin is secreted as a dimer by ...19968825766
characterization of a type iv bundle-forming pilus (sfp) from a gastroenteritis-associated strain of aeromonas veronii biovar sobria.the colonization mechanisms of enteropathogenic aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. this study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. sds-page analysis (various conditions) of pili preparations yielded a pilin protein band of approximately 21 kda. its n-terminal amino acid sequence was unambig ...19968827704
application of a modified disc diffusion technique to antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida clinical isolates.two techniques for antimicrobial susceptibility testing of vibrio anguillarum and aeromonas salmonicida strains were compared. the first method was the reference test that determines minimal inhibitory concentrations (mic); the second was a modified diffusion test that measures the inhibitory concentrations in diffusion (icd) by carrying out the diffusion test with five discs of differing contents. icd measurement was not applicable for the susceptibility testing of oxytetracycline and sulfadime ...19968828130
gram negative bacteria and contact lens induced acute red eye.two patients using hydrogel contact lenses on a daily wear schedule slept overnight with the lenses and woke up with a contact lens induced acute red eye (clare). the contact lenses recovered aseptically at the time of the event grew significant colonies of pseudomonas aeruginosa and aeromonas hydrophila in patient a and pseudomonas aeruginosa and serratia liquefaciens from patient b. similar organisms from the contact lenses were recovered from the lens case and lens care solutions of patient b ...19968828303
prolyl aminopeptidase is also present in enterobacteriaceae: cloning and sequencing of the hafnia alvei enzyme-gene and characterization of the expressed enzyme.the hafnia alvei prolyl aminopeptidase gene (hpap) was cloned and sequenced, the expressed enzyme (hpap) was purified to homogeneity and thoroughly characterized. an open reading frame of 1,281 bp was found to code for the enzyme, resulting in a protein of 427 amino acids with a molecular weight of 48,577. hpap resembles the aeromonas sobria enzyme, having 45% identity and the same distinctive properties with respect to size and substrate specificities. both enzyme show similar chromatographic b ...19968830041
the role of the o-antigen lipopolysaccharide on the colonization in vivo of the germfree chicken gut by aeromonas hydrophila serogroup o:34.we compared the ability of different aeromonas hydrophila strains from serogroup o:34 grown at different temperatures to colonize in vivo the germfree chicken gut. we found a good colonization when the strains were grown at 20 degrees c but not when they were grown at 37 degrees c. we previously described that these strains were able to form the o-antigen lipopolysaccharide (lps) when they grow at low temperature but not at high temperature. we also obtained by transposon mutagenesis mutants onl ...19968831828
[the cellular fatty acid composition of bacteria in the family vibrionaceae].it is shown that strains of vibrio cholerae of serovar o1, biovar eltor, subtype ogawa, museum strains v. cholerae of serovar o1 and nag-vibrios (isolated from various sources: sea, river and sewage water, canal water and people) possess identical composition of cell fatty acids with prevailing hexadecenoic, hexadecanoic and octadecenoic acids. being identical, fatty acid profiles of v. parahaemolyticus and v. alginolyticus, are close to that of v. cholerae differing from the latter mainly by th ...19969044701
resistance to bismuth among gram-negative bacteria is dependent upon iron and its uptake.bismuth antimicrobial action is poorly understood. many trivalent metals possess antibacterial activity, especially under low iron conditions. protection of bacteria from the deleterious effects of bismuth and other trivalent metals was demonstrated in iron-fortified media. near-equimolar quantities of fe3+ neutralized the growth-inhibitory effects of 250 microm bi3+. resistance to bismuth action also depended on the production of virulence-related siderophores. escherichia coli, aeromonas hydro ...19969023650
early onset neonatal septicemia caused by aeromonas hydrophilia. 19968979560
characterization of aeromonas hydrophila: a comparative study of strains isolated from diarrheal feces and the environment.thirty-five strains of aeromonas hydrophila isolated from feces of diarrheal patients and from the environments were collected from thailand and japan. the physiological, biochemical, and serological characteristics, antibiotic resistance patterns and cell surface-related properties were compared. the diarrheal and environmental isolates of a hydrophila were found to be remarkably consistent in general culture and biochemical characteristics, with the exception of the reaction to d-arabinose in ...19969031415
identification of aeromonas hydrophila hybridization group 1 by pcr assays.synthetic oligonucleotide primers of 24 and 23 bases were used in a pcr assay to amplify a sequence of the lip gene, which encodes a thermostable extracellular lipase of aeromonas hydrophila. a dna fragment of approximately 760 bp was amplified from both sources, i.e., lysed a. hydrophila cells and isolated dna. the amplified sequence was detected in ethidium bromide-stained agarose gels or by southern blot analysis with an internal hindiii-bamhi 356-bp fragment as a hybridization probe. with a. ...19968919777
camp test for the identification of vibrio cholerae 0139.the confirmation of the identity of vibrio cholerae serogroup 01 and serogroup 0139 is usually done by slide agglutination tests using specific antisera. antiserum to v. cholerae 01 is freely available but not antiserum to v. cholerae 0139, thus making specific identification of the latter more difficult. a modified camp (christie atkins and muench - paterson) test has been described as a possibility in the identification of v. cholerae 0139 and we have evaluated this on 197 strains of organisms ...19968926028
aeromonas trota strains, which agglutinate with vibrio cholerae o139 bengal antiserum, possess a serologically distinct fimbrial colonization factor.pili of aeromonas trota strain 1220, which agglutinates with vibrio cholerae o139 bengal antiserum, were purified and characterized. the molecular mass of the subunit protein was estimated to be 20 kda and the pl was 5 center dot 4. the pili were immunologically unrelated to the other aeromonas pili reported so far. however, the n-terminal amino acid sequence of the subunit pilin was similar to those of the pilins from other aeromonas pili reported previously. neither a. trota cells nor pili pur ...19968932704
measurement of virulence of aeromonads using a suckling mouse model of infection.virulent and avirulent strains of aeromonas spp. were identified and virulence quantified using an animal model. virulence was measured by determining a 50% lethal dose (ld50) 43 h after oral administration of live bacteria. the ld50 of virulent aeromonas isolates ranged from log10 7.53 (mean) organisms to log10 8.88 (mean). some isolates were avirulent in this model. detection of cytotoxic activity in culture supernatants correlated with virulence (fisher exact test, p = 0.0029). there was no c ...19968908484
an aeromonas salmonicida gene required for the establishment of infection in rainbow trout (oncorhynchus mykiss).the asob gene of aeromonas salmonicida is located approximately 9 kb downstream of the structural gene (vapa) for the surface layer (a-layer). the nucleotide sequence of asob was determined and found to encode a putative polytopic cytoplasmic membrane protein which exhibited homology to a number of bacterial transport proteins. allele exchange mutagenesis of asob resulted in a mutant (a449-d) which was avirulent when administered by bath immersion. however, when administered by intraperitoneal i ...19968917088
cloning, characterization and expression of the reca gene of aeromonas salmonicida.the aeromonas salmonicida a449 reca gene has been cloned, sequenced and expressed in vitro. the predicted amino acid sequence of a. salmonicida reca was determined and, when compared to other reca, was found to possess a number of domains identical to those characterized in escherichia coli reca. the a. salmonicida reca was mobilized into an e. coli reca mutant strain and was shown to allow increased survival in the presence of the chemical mutagen mms and after ultraviolet (uv) irradiation. the ...19968917089
characterization of a stable l-form of the fish pathogen aeromonas salmonicida.a stable l-form of aeromonas salmonicida, which resulted from induction with benzylpenicillin, contained more of an outer membrane protein, with an estimated molecular weight of 40 kda but less of 47.9 and 38 kda proteins, than did parental walled cells. in addition, from western blots, two protein bands reacted strongly with a polyclonal antiserum. the antiserum did not react demonstrably with the band detected in the l-forms on the gels.19968987904
experiences with different vaccines and vaccination procedures to control furunculosis in sea-reared atlantic salmon. 19968996887
biochemical characterization of the carbapenem-hydrolyzing beta-lactamase asbm1 from aeromonas sobria aer 14m: a member of a novel subgroup of metallo-beta-lactamases.asbm1, a carbapenem-hydrolyzing beta-lactamase produced by aeromonas sobria aer 14m, was purified chromatographically, with anion exchange chromatography performed in the absence of zn2+. the molecular mass of asbm1 was approximately 34,000; the isoelectric point was 9.1. asbm1 had high hydrolytic specificity for carbapenems but low hydrolysis rates for penicillins and cephalosporins. asbm1 was resistant to the commercially available beta-lactamase inhibitors but was inhibited by pcmb and the ch ...19968998985
[rapid detection of the hemolysin genes in aeromonas sobria by the polymerase chain reaction].the hemolysin of aeromonas sobria is one of the important virulence factors in this organism. rapid detection and identification test for a. sobria is important for early and specific diagnosis of this infectious disease. we evaluated the polymerase chain reaction (pcr) for the rapid detection of a. sobria. two pairs of synthetic oligonucleotide primers (asa1-s and a; aeraas-s and a) were used in pcr technique to detect the different hemolysin genes (asa1 and aeraas) in a. sobria. the pcr identi ...19969011120
[endophthalmitis caused by aeromonas sobria]. 19969011213
[aeromonas hydrophila rapidly progressive myonecrosis]. 19969017903
[cytolysins and homologous proteins produced by gram-negative bacteria. ii. hemolysins of aeromonas, serratia, proteus and morganella and proteins from gram-negative bacteria related to cytolysins].this article presents the genetic determination, synthesis and activation of hemolysins produced by mentioned above bacteria, as well as the possible role of these toxins in the pathogenicity. all these cytolysins are related to rtx proteins presented to the previous paper. the short information about cytotoxic proteins from other gram-negative bacteria (p. aeruginosa, k. pneumoniae, vibrionaceae, g. vaginalis) and homologous proteins from e. chrysanthemi and r. leguminosarum biovar viciae are a ...19969019745
the effect of temperature and water quality on antibody response to aeromonas salmonicida in sunshine bass (morone chrysops x morone saxatilis).the kinetics of the antibody response to aeromonas salmonicida were determined in sunshine bass (hybrid striped bass: female morone chrysops x male morone saxatilis) acclimated to 10, 18, 24, 29 degrees c, and to 0.15 mg 1(-1) un-ionized ammonia and 200 mg 1(-1) nitrate levels. temperature and water quality factors affected the immune response of sunshine bass. temperatures of 10 degrees c and 18 degrees c decreased the magnitude and delayed the time of the antibody response to a. salmonicida. t ...19969157680
the aeromonas metallo-beta-lactamases: genetics, enzymology, and contribution to drug resistance.aeromonads are environmental microorganisms that can be responsible for both human and animal infections. individual aeromonas strains can produce up to three different, inducible, chromosomally encoded beta-lactamases, including a group 1 molecular class c cephalosporinase, a group 2d molecular class d penicillinase, and a group 3 molecular class b metallo-beta-lactamase, which contribute to beta-lactam resistance in members of this genus. among these enzymes, the metallo-beta-lactamases are cl ...19969158767
overproduction and purification of the aeromonas hydrophila cpha metallo-beta-lactamase expressed in escherichia coli.the aeromonas hydrophila cpha metallo-beta-lactamase was overexpressed in a soluble secreted form in escherichia coli using a t7 rna polymerase-based expression system, and a simple protocol based on a single cation-exchange chromatographic step was developed, which is suitable for rapid purification of the overexpressed enzyme from e. coli lysates. a yield of up to 30 micrograms of purified enzyme per milliliter of culture was obtained. the purified enzyme preparation showed properties identica ...19969158768
diffusion of carbapenems through the outer membrane of enterobacteriaceae and correlation of their activities with their periplasmic concentrations.scarce information is available on the real mechanism by which carbapenemes penetrate in enterobacteriaceae, although a considerable amount of evidence suggests that in many species of this family the lack of certain outer membrane proteins is associated with the acquisition of resistance to these antibiotics. the existance of specific pathways for the carbapenems has never been demonstrated, although at times it has been postulated in both wild and mutant strains, on the basis of evident discor ...19969158772
behaviour of aeromonas spp. after animal passage. 19969070410
effects of modified atmosphere and vacuum packaging on the growth of spoilage and inoculated pathogenic bacteria on fresh poultry.fresh chicken breast meats inoculated with yersinia enterocolitica and aeromonas hydrophila were packaged in glass jars either containing different compositions of modified atmospheres (ma) (100% co2; 80% co2/20% n2), or in vacuo or containing air, and were stored at 3 +/- 1 degrees c and 8 +/- 1 degrees c. the changes in gas composition as well as y. enterocolitica, a. hydrophila, total aerobic bacterial, total psychotropic, lactobacilli and enterobacteriaceae counts were determined after 0, 1, ...19969123970
the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis.from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ...19969147724
hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a pseudomonas species.hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera pseudomonas, moraxella, arthrobacter and aeromonas. a hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the pseudomonas and aeromonas spp. and in total soluble fractions of the arthrobacter sp. a monomeric 19-kda non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the pseudomonas species and shown to be simi ...19969082922
application of a colorimetric dna-dna hybridization method for identification of aeromonas genomic species, isolated from diarrheal stools.the colorimetric dna-dna hybridization method for the identification of 18 strains of aeromonas spp. isolated from human stools was used. bacterial isolates were also examined by phenotypic characteristics. on the basis of biochemical tests 13 strains were included in phenogroup a. caviae and 5 strains in a. sobria. identification to the species level was obtained by colorimetric hybridization method. dna-dna similarity values showed that isolates of a. caviae group belong to hybridization group ...19969131804
rapid and sensitive method for the detection of aeromonas caviae and aeromonas trota by polymerase chain reaction.a 16s rdna-based polymerase chain reaction (pcr) method was developed for the detection of aeromonas caviae and aeromonas trota. these two species were identified from other aeromonas spp. and closely related species by primers set (aer1 and aer2). the amplified product was 316 bp. the identity of the amplified product was confirmed by dna-dna hybridization. two sets of primers (aer8 and aer9) were used for specific identification of aer. caviae. amplifying the 260 bp fragment of 16s rrna gene r ...19979134769
[aeromonas hydrophila bone infection]. 19979137396
in vivo production of a-protein, lipopolysaccharide, iron-regulated outer membrane proteins and 70-kda serine protease by aeromonas salmonicida subsp. salmonicida.using specific immunostaining of western blots, the in vivo expression of several putative virulence factors of aeromonas salmonicida subsp. salmonicida was demonstrated in infected muscle tissue of atlantic salmon and rainbow trout. three virulent isolates of a. salmonicida were used. one isolate was chosen because in vitro it was apparently a non-producer of the 70-kda serine protease. infected furuncle tissue was centrifuged and samples of the pellet and supernatant probed for evidence that t ...19979141656
plasmids and aeromonas virulence.most putative virulence determinants of aeromonas species are chromosomally encoded. however, several recent reports have indicated that some may be carried on or regulated by plasmids. therefore, we examined the plasmid carriage rate of a total of 140 clinical and environmental aeromonas isolates. plasmid carriage was compared with the ability of an isolate to produce toxins and adhere to hep-2 cells. overall, plasmid incidence in aeromonas species was low (23/140, 16%) and independent of the s ...19979143879
enteropathogenicity of aeromonas jandaei and a. experimental study of five isolates of aeromonas jandaei and 12 of a. trota was carried out to examine if they produced an enterotoxic substance, and if so, to characterise that factor and to see if it caused any mucosal damage. only two of the a. trota strains caused fluid accumulation in the initial rabbit ileal loop (ril) tests. the remaining strains did so only after one to five sequential passages through rils and once they caused a secretory response they showed a gradual enhancement of ...19979143882
aeromonas bacteremia in patients with hematologic diseases.over a 23-year period, 17 patients with hematologic diseases developed aeromonas bacteremia while in our hematology ward. male predominance (14 patients, 82%) was seen, with a predilection for the elderly. hematologic malignancies, especially acute leukemia, accounted for 15 (88%) of all patients. cancer chemotherapy and neutropenia (15 patients each) were the most common preceding host conditions. aeromonas bacteremia generally occurred in the second half of the year (july-december), with no ex ...19979144007
[conjunctivitis caused by aeromonas hydrophila]. 19979147511
crystal structure of carboxypeptidase g2, a bacterial enzyme with applications in cancer therapy.carboxypeptidase g enzymes hydrolyze the c-terminal glutamate moiety from folic acid and its analogues, such as methotrexate. the enzyme studied here, carboxypeptidase g2 (cpg2), is a dimeric zinc-dependent exopeptidase produced by pseudomonas sp. strain rs-16. cpg2 has applications in cancer therapy: following its administration as an immunoconjugate, in which cpg2 is linked to an antibody to a tumour-specific antigen, it can enzymatically convert subsequently administered inactive prodrugs to ...19979083113
molecular analysis and expression of the extracellular lipase of aeromonas hydrophila mcc-2.the structural gene encoding the extracellular lipase of aeromonas hydrophila mcc-2 was cloned and found to be expressed in escherichia coli using its own promoter. when the cloned gene (lip) was expressed in e. coli minicells, an 80 kda protein was identified. subcellular fractionation of e. coli carrying the lip gene indicated that the lip protein was mainly associated with the membrane fraction. nucleotide sequence analysis revealed that the gene is 2253 bp long, coding for a 79-9 kda protein ...19979084164
sensitivity of aeromonas hydrophila carbapenemase to delta3-cephems: comparative study with other metallo-beta-lactamases.ceftriaxone and ceftriaxone s-oxide behaved as inactivators against the metallo-beta-lactamase of aeromonas hydrophila ae036 and as substrates for the zinc beta-lactamase produced by bacillus cereus (569/h/9) and stenotrophomonas maltophilia ula 511. moreover, ro 09-1428, a catechol-cephalosporin, was not recognized by the a. hydrophila enzyme. panipenem, cephalosporin c, cephalosporin c-gamma-lactone, and loracarbef were substrates for the three studied beta-lactamases.19979087509
[severe acute gastroenteritis due to aeromonas in a patient colectomized for crohn's disease].we report a case of severe acute diarrhoea produced by aeromonas sobria, a specimen with controversial human pathogenicity. a 26-year-old man with crohn's disease previously colectomized was admitted with a cholera-like clinical presentation and renal failure. aeromonas sp. can be isolated in both children and adults and in healthy people, a. hydrophila being most often detected. severe infections due to other aeromonas sp., usually a. sobria and a. caviae, have been rarely described. most patie ...19979090982
transcription analysis of the staphylococcus aureus gene encoding penicillin-binding protein 4.the high level of cross-linking found in staphylococcus aureus peptidoglycan is dependent on the low-molecular-weight penicillin-binding protein pbp4. recently, the pbp4 gene, pbpd, was cloned and shown to be adjacent to and divergently transcribed relative to the putative abc-type transporter gene, abca. disruption of abca (in strain kb400) was previously shown to result in heightened resistance to several antibiotics known to interact with pbp4, suggesting that the regulation of pbpd is affect ...19979098064
mechanistic studies on the aminopeptidase from aeromonas proteolytica: a two-metal ion mechanism for peptide hydrolysis.the aminopeptidase from aeromonas proteolytica (aap) is uncompetitively inhibited by fluoride ion at ph 8.0 with an inhibition constant (ki) of 30 mm. thus, fluoride inactivates aap only after substrate binding, and only a single fluoride ion binds to aap. on the other hand, chloride ion does not inhibit aap up to concentrations of 2 m at ph 8.0. the ph dependence of fluoride inhibition of aap was measured over the ph range 6.0-9.5. between ph values of 6.0 and 9.0, fluoride ion acts as a pure u ...19979100023
pathophysiologic plasma levels of leukotriene c4 in relation to the hemodynamic dysfunction and mediator release of graded bacteremia.this study was undertaken to identify those events of bacteremic shock that pathophysiologic levels of leukotriene c4 (ltc4) alone were sufficient to cause. sixteen adult swine were studied for 4 h in three groups: anes (n = 6) received anesthesia only; septic (n = 6) received aeromonas hydrophila, 10(9)/ml, intravenously, increased incrementally from .2 to 4.0 ml/kg/h; ltc4 (n = 4) received ltc4 infused intravenously, at rates that approximated ltc4 levels of septic animals. measurements includ ...19979110414
studies on the energetics of proaerolysin secretion across the outer membrane of aeromonas species. evidence for a requirement for both the protonmotive force and atp.aeromonas spp. secrete the channel-forming protein proaerolysin across their inner and outer membranes in separate steps using the general secretion pathway. here we show that treating a. hydrophila or a. salmonicida with the protonophore carbonyl cyanide m-chorophenyl hydrazone blocks the second step in transport, secretion across the outer membrane from the periplasm, under conditions where the atp levels in the cell are no different than the levels in control, secreting cells. a threshold for ...19979111006
fatal myofascial necrosis due to imipenem-resistant aeromonas hydrophila.we report the case of a fatal myofascial necrosis caused by an imipenem-resistant aeromonas hydrophila in a patient with a history of aplastic anemia. he presented with fever and left thigh tenderness. the ct scan was consistent with cellulitis and, after cultures were obtained, empirical treatment with imipenem and amikacin was started. two days later, necrotic bullae appeared on his thigh, and cultures showed aeromonas hydrophila which was imipenem-resistant. although surgical debridement was ...19979112306
the glycosylphosphatidylinositol-anchored surface glycoprotein thy-1 is a receptor for the channel-forming toxin aerolysin.aerolysin is a channel-forming protein secreted by virulent aeromonas spp. some eucaryotic cells, including t-lymphocytes, are sensitive to very low concentrations of the toxin (<10(-9) m). here we show that aerolysin binds selectively and with high affinity to the glycosylphosphatidylinositol (gpi)-anchored surface protein thy-1, which is found on t-lymphocyte populations as well as in brain. less than 1 ng of purified thy-1 could be detected by probing western blots with the toxin. mutant t-ce ...19979115289
influence of osmolarity on lipopolysaccharides and virulence of aeromonas hydrophila serotype o:34 strains grown at 37 degrees c.growth of aeromonas hydrophila serotype o:34 strains at 37 degrees c at low and high osmolarity resulted in changes in the lipopolysaccharide (lps) and virulence of the strains tested. we previously described the effect of growth temperature on lps and virulence of these strains (s. merino et al., infect. immun. 60:4343-4349, 1992). the effect of osmolarity can be observed when the cells grow at 37 degrees c but not when they grow at 20 degrees c. purified lps from cells cultivated at 37 degrees ...19979119458
the disulfide bond in the aeromonas hydrophila lipase/acyltransferase stabilizes the structure but is not required for secretion or activity.vibrio and aeromonas spp. secrete an unusual 35-kda lipase that shares several properties with mammalian lecithin-cholesterol acyltransferase. the aeromonas hydrophila lipase contains two cysteine residues that form an intramolecular disulfide bridge. here we show that changing either of the cysteines to serine does not reduce enzymatic activity, indicating that the disulfide bond is not required for correct folding. however, when either of the cysteines is replaced, the enzyme is more readily d ...19979150203
the gly1 gene of saccharomyces cerevisiae encodes a low-specific l-threonine aldolase that catalyzes cleavage of l-allo-threonine and l-threonine to glycine--expression of the gene in escherichia coli and purification and characterization of the enzyme.the gly1 gene of saccharomyces cerevisiae is required for the biosynthesis of glycine for cell growth [mcneil, j. b., mcintosh, e. v., taylor, b. v., zhang, f-r., tang, s. & bognar, a. l. (1994) j. biol. chem. 269, 9155-9165], but its gene product has not been identified. we have found that the gly1 protein is similar in primary structure to l-allo-threonine aldolase of aeromonas jandiae dk-39, which stereospecifically catalyzes the interconversion of l-allo-threonine and glycine. the gly1 gene ...19979151955
evaluation of caribbean strains of macrocyclops and mesocyclops (cyclopoida:cyclopidae) as biological control tools for the dengue vector aedes aegypti.fifteen caribbean strains of copepods were assessed for their predation ability against mosquito larvae. macrocyclops albidus from nariva. mesocyclops aspericornis from oropouche, and mesocyclops longisetus from e1 socorro, trinidad, were most effective against aedes aegypti but not against culex quinquefasciatus. mesocyclops longisetus and me. aspericornis prevented any mosquito survival over 25 wk of observation despite weekly challenges with ae. aegypti. the copepods were tolerant to dosages ...19979152870
infections after shark attacks in australia. 19979154554
[recent knowledge on fish pathogenic bacteria aeromonas salmonicida, listonella anguillara and cytophaga columnaris, and their virulence factors]. 19979155200
[studies on enrichment broth for verotoxin-producing escherichia coli (vtec) o157].growth of verotoxin-producing escherichia coli (vtec) o157 in conventionally recommended pre-enrichment broth media at different temperatures was evaluated. in addition, sensitivity of vtec o157 isolates to several antibacterial drugs, which were added to the selective enrichment broth, was tested. all five isolates of vtec o157 tested grew well in trypticase soy broth (tsb) at 36 degrees c and 42 degrees c, while the growth of one isolate was markedly suppressed in tsb supplemented with cefixim ...19979155207
[piperacillin/tazobactam in the treatment of abdominal sepsis in patients with peritonitis].piperacillin/tazobactam (p/t) was used in the treatment of 40 patients at the age of 22 to 64 years with intraabdominal infection (peritonitis, abscesses). p/t was administered as 30-minute intravenous infusions 4 or 3 times a day for 3 to 13 days. the recovery and improvement were stated in 80 and 10 per cent of the cases respectively. in 10 per cent of the patients the treatment failed because of surgical complications or insufficient surgical intervention or because of some other systemic dis ...19979124988
complement resistance of capsulated strains of aeromonas salmonicida.the complement resistance of aeromonas salmonicida strains grown under conditions promoting capsule formation was investigated using well characterized strains and their isogenic mutants. complement resistance was previously studied using the same strains growing under non-capsulating conditions. the serum resistant strains were found to activate complement, but rapidly degrade c3b preventing productive formation of the lytic complex c5b-9. isogenic lipopolysaccharide rough mutants grown under n ...19979160301
cloning and characterization of two immunophilin-like genes, ilpa and fkpa, on a single 3.9-kilobase fragment of aeromonas hydrophila genomic dna.antiserum to aeromonas hydrophila a6 cell envelopes was shown in a previous study (c. y. f. wong, g. mayrhofer, m. w. heuzenroeder, h. m. atkinson, d. m. quinn, and r. l. p. flower, fems immunol. med. microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. in this study, colony blot analysis of an a. hydrophila genomic library using antiserum to a. hydrophila a6 cell envelopes revealed a cosmid clone expressing a 30-kda protein which has not been described previou ...19979171380
l-allo-threonine aldolase from aeromonas jandaei dk-39: gene cloning, nucleotide sequencing, and identification of the pyridoxal 5'-phosphate-binding lysine residue by site-directed mutagenesis.we have isolated the gene encoding l-allo-threonine aldolase (l-allo-ta) from aeromonas jandaei dk-39, a pyridoxal 5'-phosphate (plp)-dependent enzyme that stereospecifically catalyzes the interconversion of l-allo-threonine and glycine. the gene contains an open reading frame consisting of 1,014 nucleotides corresponding to 338 amino acid residues. the protein molecular weight was estimated to be 36,294, which is in good agreement with the subunit molecular weight of the enzyme determined by po ...19979171400
identification of a second endogenous porphyromonas gingivalis insertion this study a second endogenous porphyromonas gingivalis insertion element (is element) that is capable of transposition within p. gingivalis was identified. nucleotide sequence analysis of the tn4351 insertion site in a p. gingivalis tn4351-generated transconjugant showed that a complete copy of the previously unidentified is element, designated pgis2, had inserted into is4351r in tn4351. pgis2 is 1,207 bp in length with 19-bp imperfect terminal inverted repeats, and insertion resulted in a d ...19979171437
pril-ampicillin-dextrin-ethanol agar for the isolation and quantification of aeromonas spp. from polluted environmental waters.several selective media were evaluated for their suitability for the isolation and quantification of mesophilic aeromonas species from naturally polluted samples. satisfactory recoveries were obtained with most of them but only when densities of background microflora were low. when analysed samples were from highly polluted waters, results were inconsistent because they did not give quantitative recovery of mesophilic aeromonads or they did not permit ready differentiation of aeromonas species f ...19979172397
carbapenem-hydrolyzing beta-lactamases. 19979021171
characterization of aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in bangladesh.aeromonas isolates from patients with diarrhea in bangladesh (n = 69), from healthy controls (n = 11), and from surface water (n = 40) were analyzed with respect to their hybridization groups (hgs) by the aid of fatty acid methyl ester (fame) characterization and dna fingerprinting by aflp, biochemical phenotypes (phe-neplate [php] types), and the production of hemolysin and cytotoxin. the aim of the investigation was to find out whether certain strains carrying virulence factors predominated am ...19979003598
production of monoclonal antibodies to the non-membrane-damaging cytotoxin (nmdcy) purified from vibrio cholerae o26 and distribution of nmdcy among strains of vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay.the distribution of a newly described secretogenic non-membrane-damaging cytotoxin (nmdcy) among strains of vibrio cholerae and other enteric bacteria was determined. to accomplish this, monoclonal antibodies against nmdcy were prepared and a sandwich monoclonal-polyclonal enzyme-linked immunosorbent assay (elisa) was developed. by the sandwich elisa, it was determined that 55.6% of the 412 strains of v. cholerae examined produced nmdcy at varying concentrations while 76, 37.9, and 15.6% of the ...19979009346
aeromonas hydrophila soft-tissue infection: a report of two cases. 19979043548
inhibition of metallo-beta-lactamases by a series of mercaptoacetic acid thiol ester derivatives.a series of mercaptoacetic acid thiol esters have been identified as metallo-beta-lactamase inhibitors. electrospray mass spectrometry (esms) has shown that irreversible inhibition of the bacillus cereus ii metallo-beta-lactamase by sb214751, sb214752, and sb213079 was concomitant with a 90-da increase in mass of the enzyme. tryptic digestion of the b. cereus ii inhibited with sb214751 illustrated that the peptide fragment, containing the only cysteine of the enzyme, had undergone a mass increme ...19978980769
an enzyme-linked immunosorbent assay for detection of vibrio vulnificus biotype 2: development and field studies.vibrio vulnificus biotype 2 is a primary eel pathogen which constitutes a lipopolysaccharide (lps)-based homogeneous o serogroup within the species. in the present work, we have developed an enzyme-linked immunosorbent assay (elisa) based on the specificity of lps for the detection of this pathogen. the elisa specificity was confirmed after testing 36 biotype 2 strains from laboratory cultures and environmental samples, 31 clinical and environmental biotype 1 isolates, and several strains of vib ...19979023934
isolation and characterization of chitinase from a flake-chitin degrading marine bacterium, aeromonas hydrophila h-2330.a flake-chitin degrading marine bacterium was isolated and identified as aeromonas hydrophila. this strain secreted five chitinases and an beta-n-acetylglucosaminidase. the main chitinase (chi-a) was purified and characterized. the optimum ph of chi-a was 5-8, and the activity was inhibited by hg2+ and fe3+. chi-a was different from chitinases of other aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. the amino-terminal amino acid sequence showed e ...19979028048
aeromonas species in fish, fish-eggs, shrimp and freshwater.aeromonas spp. are common contaminants of fish and seafood. they also are ubiquitous in the water environment. aeromonas spp. were identified in 27 (93%) of 29 fish, in 17 (100%) fish-egg, in two (16%) of 12 shrimp samples and in 23 (100%) freshwater samples. in total, 117 aeromonas strains were isolated from 69 positive samples, several samples having had two or three aeromonas species. included in this were also 26 mesophilic aeromonas strains isolated in association with the study on fish dis ...19979029253
streptomyces griseus aminopeptidase: x-ray crystallographic structure at 1.75 a resolution.the x-ray crystal structure of the enzyme streptomyces griseus aminopeptidase (sgap) has been determined in its double zinc form to 1.75 a resolution, in its apo-enzyme from (zinc removed) to 2.1 a resolution, and as a mercury replaced derivative to 2.1 a resolution. the structure solution was achieved by single isomorphous replacement with phasing from anomalous scattering (siras), followed by density modification with histogram matching. the protein consists of a central beta-sheet made up of ...19979048953
a 4-year study of the diversity and persistence of coliforms and aeromonas in the water of a swedish drinking water well.coliforms and aeromonas, isolated over a sampling period of 4 years from a swedish drinking water well, were analysed for their phenotypical diversity and for their ability to persist in the well water. from each of the 40 water samples collected from the well, 32 bacterial isolates were subjected to typing by the pheneplate (php) biochemical fingerprinting system. strains able to persist in the well water were further characterized using the api 20e system, gas-liquid chromatographic cellular f ...19979057291
antibiotic-induced antimicrobial resistance in aeromonas spp. 19979059443
expression of the asba1, oxa-12, and asbm1 beta-lactamases in aeromonas jandaei aer 14 is coordinated by a two-component regulon.aeromonas jandaei aer 14 (formerly aeromonas sobria aer 14) expresses three inducible beta-lactamases, asba1, oxa-12 (asbb1), and asbm1. mutant strains that constitutively overexpress all three enzyme simultaneously, suggesting that they share a common regulatory pathway, have been isolated. detectable expression of the cloned genes of asba1 and oxa-12 in some escherichia coli k-12 laboratory strains is achieved only in the presence of a blp mutation. these mutations map to the cre operon at 0 m ...19979068648
the aerobic growth of aeromonas hydrophila and listeria monocytogenes in broths and on pork.flasks of tryptic soy broth (tsb), unacidified (ph 7.2) or acidified with hcl or lactic acid to ph 6.3 or 5.5, and samples of sterile pork fat or muscle tissue, were inoculated with logarithmic phase cultures of a strain of aeromonas hydrophila or a strain of listeria monocytogenes. the broth cultures were incubated at temperatures between 0 and 25 degrees c, and growth rates were determined from optical density increases. the tissue samples were incubated at temperatures between -2.4 and 25.2 d ...19979081227
production of amylase by the intestinal microflora in cultured freshwater fish.the amylase-producing ability of the intestinal microflora in cultured specimens of ayu, carp, channel catfish, japanese eel and tilapia was determined. mean viable counts of aerobes and anaerobes ranged from 1.1 x 10(6) to 3.7 x 10(8) cfu g-1 and from 1.3 x 10(3) to 1.6 x 10(8) cfu g-1, respectively. aeromonas spp. and bacteroidaceae were predominant in four to five fish species. of 206 strains examined, 65 (31.6%) produced > or = 0.01 u amylase ml-1. the percentage of producers differed among ...19979081303
[isolation and characteristics of plasmids from aeromonas].fourteen out of fifty-two examined strains of aeromonas contain plasmids. one of them determines the resistance to tetracycline, streptomycin, and chloramphenicol. the pas30 plasmid, 53 t. n. p. in size, is capable of conjugative transfer to bacteria of different genera, which permits us to consider it a broad host range plasmid. using 12 restriction endonucleases, the physical map of the plasmid was plotted, on which the genes responsible for transporting functions and streptomycin resistance w ...19979082183
acute phase proteins in salmonids: evolutionary analyses and acute phase response.inflammation induces dramatic changes in the biosynthetic profile of the liver, leading to increased serum concentrations of positive acute phase (ap) proteins and decreased concentrations of negative ap proteins. serum amyloid a (saa) and the pentraxins c-reactive protein (crp) and serum amyloid p component (sap) are major ap proteins: their serum levels can rise by 1000-fold, indicating that they play a critical role in defense and/or the restoration of homeostasis. we have cloned saa and a sa ...19978977214
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