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haemolytic-uraemic syndrome in an adult male with aeromonas hydrophila enterocolitis. 199910069207
mortality of captive canadian toads from basidiobolus ranarum mycotic dermatitis.twenty-six adult free-ranging canadian toads (bufo hemiophrys) were collected from northeastern north dakota (usa) during the last week of august 1994 and placed in captivity. during late december and january 1995, 21 canadian toads died. clinical signs included increased time sitting in water bowls, darkened dorsal skin, constant arching of their backs, and hyperemia and sloughing of ventral epidermis. the condition progressively worsened until death occurred within 5 to 7 days after onset of c ...199910073347
identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr.molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ...199910074520
[aeromonas septicemia]. 199910088391
peroxide-inducible catalase in aeromonas salmonicida subsp. salmonicida protects against exogenous hydrogen peroxide and killing by activated rainbow trout, oncorhynchus mykiss l., macrophages.aeromonas salmonicida subsp. salmonicida expresses a single cytoplasmically located catalase which was found to be inducible by exposure to 20 microm hydrogen peroxide in mid-exponential phase resulting in a 4 fold increase in activity. subsequent exposure to 2 mm peroxide in late-exponential/early-stationary phase resulted in further induction of catalase activity which increased to 20 fold higher levels than those found in uninduced cultures. exponentially induced cultures were protected again ...199910089155
the type iv aeromonas pilus (tap) gene cluster is widely conserved in aeromonas species.nothing is known regarding the expression or function of the type iv aeromonas pilus (tap), which was recently identified following the cloning of a pilus biogenesis gene cluster (tapabcd). as a first step to determine the possible significance of tap for aeromonas virulence, the distribution of the tapa and tapd genes in hybridization group reference strains and clinical (n=42) and environmental (n=29) isolates was determined. homologues of tapa and tapd were present in all strains tested. hybr ...199910090854
catecholamine enhancement of aeromonas hydrophila growth.several species of bacteria have been shown to respond to the administration of norepinephrine and other catecholamines with increased growth (in culture) and virulence. in this study, we examined the effects of catecholamines on the growth of cultures of aeromonas hydrophila, a gram-negative bacillus found in brackish water. bacterial cultures were maintained in tryptic soy both, then washed free of medium and transferred to a bovine serum-supplemented minimal salts medium. treatment of a. hydr ...199910090855
small subunit rrna gene sequences of aeromonas salmonicida subsp. smithia and haemophilus piscium reveal pronounced similarities with a. salmonicida subsp. salmonicida.the small subunit ribosomal rna (ssu rrna) encoding genes from reference strains of aeromonas salmonicida subsp. smithia and haemophilus piscium were amplified by polymerase chain reaction and cloned into escherichia coli cells. almost the entire ssu rrna gene sequence (1505 nucleotides) from both organisms was determined. these dna sequences were compared with those previously described from a. salmonicida subsp. salmonicida, subsp. achromogenes and subsp. masoucida. this genetic analysis revea ...199910092980
appearance of imp-1 metallo-beta-lactamase in europe. 199910093989
copper exposure reduced the resistance of the catfish saccobranchus fossilis to aeromonas hydrophila infection. 199910094734
aeromonas species isolated from medicinal leeches.aeromonas hydrophila infections are a recognized complication of the use of medicinal leeches. the authors performed an experiment designed to find a safe and practical way to sterilize the leech gut of pathogenic organisms. leeches were incubated for a 12-hour period in solutions of antibiotic effective against a. hydrophila. the incubations in the antibiotic solutions failed to eradicate pathogenic bacteria from the gut of the leeches. the authors examined cultures of bacteria isolated from th ...199910096618
aeromonas hydrophila isolated from cases of bovine seminal vesiculitis in south brazil. 199910098696
secretion of hemolysin of aeromonas sobria as protoxin and contribution of the propeptide region removed from the protoxin to the proteolytic stability of the toxin.the sequence at the amino terminus region of the hemolysin ofaeromonas sobria is homologous with that of aerolysin of a. hydrophila. however, there is no homology between the two toxins in the sequence at the carboxy terminal region. it has been shown that aerolysin is secreted into culture supernatant as a protoxin. this proaerolysin is activated by the proteolytic removal of a carboxy terminal peptide. however, the role of the carboxy terminal region, which is removed in the activation process ...199910100744
purification and characterization of cold-active l-glutamate dehydrogenase independent of nad(p) and oxygen.l-glutamate dehydrogenase (gldh) independent of nad(p) and oxygen was first obtained from the psychrotrophic bacterium aeromonas sp. l101, originally isolated from the organs of salmon (oncorhynchus keta). gldh was purified by a series of chromatography steps on deae-sepharose, superdex 200pg, q-sepharose, cm-sepharose, and phenyl-sepharose. the purified protein was determined to have a molecular mass of 110 kda and a pi of 5.7. maximum activity was obtained at 55 degrees c and ph 8.5. the activ ...199910101290
symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations.hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. infections by aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered. the associated infections demonstrate the need for a better understanding of the digestive tract flora of h. medicinalis. early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of bl ...19999864188
an in-vitro evaluation of the activity of chlorine against environmental and nosocomial isolates of aeromonas hydrophila.a chlorine preparation was tested against 34 strains of aeromonas hydrophila isolated from a hospital water facility and patients with nosocomial infections in a french medical school hospital. in-vitro bactericidal activity was determined by a macro- and micromethod, using specific interfering substances over a range of dilutions and temperatures, after 1 and 5 minute exposure times. a 10(5)-fold reduction in the challenge inoculum was taken as the criterion of efficacy. results obtained by bot ...19999949964
antimicrobial susceptibility patterns of aeromonas jandaei, a. schubertii, a. trota, and a. veronii biotype veronii.fifty-six isolates of four aeromonas species, which have been documented as causative agents of human infections or isolated from human clinical specimens, were subjected to antimicrobial susceptibility testing using a microscan walkaway conventional (overnight incubation) gram-negative panel. the four species tested and the number of isolates of each were as follows: aeromonas jandaei, 17; a. schubertii, 12; a. trota, 15; and a. veronii biotype veronii, 12. all isolates of a. trota were suscept ...19999986836
structure of in31, a blaimp-containing pseudomonas aeruginosa integron phyletically related to in5, which carries an unusual array of gene cassettes.the location and environment of the acquired blaimp gene, which encodes the imp-1 metallo-beta-lactamase, were investigated in a japanese pseudomonas aeruginosa clinical isolate (isolate 101/1477) that produced the enzyme. in this isolate, blaimp was carried on a 36-kb plasmid, and similar to the identical alleles found in serratia marcescens and klebsiella pneumoniae clinical isolates, it was located on a mobile gene cassette inserted into an integron. the entire structure of this integron, nam ...199910103196
biochemical characterization of the pseudomonas aeruginosa 101/1477 metallo-beta-lactamase imp-1 produced by escherichia coli.the blaimp gene coding for the imp-1 metallo-beta-lactamase produced by a pseudomonas aeruginosa clinical isolate (isolate 101/1477) was overexpressed via a t7 expression system in escherichia coli bl21 (de3), and its product was purified to homogeneity with a final yield of 35 mg/liter of culture. the structural and functional properties of the enzyme purified from e. coli were identical to those of the enzyme produced by p. aeruginosa. the imp-1 metallo-beta-lactamase exhibits a broad-spectrum ...199910103197
signature region within the 16s rdna sequences of aeromonas popoffii.to identify a group of eight aeromonas strains of our collection showing ribotyping patterns similar to those described for the species aeromonas popoffii, 16s rrna gene sequence analysis was performed. results were in agreement with the dna binding values, and allowed the identification of a 'signature region' differentiating the a. popoffii strains from all other members of the genus aeromonas.199910188252
[antimicrobial resistance among species of salmonella, shigella, escherichia, and aeromonas isolated from children with diarrhea in 7 argentinian centers].the increasing levels of resistance of enteropathogenic bacteria against antimicrobial agents present geographic variations. we have analysed the antimicrobial susceptibility of isolates obtained from 4,364 children under 5 years of age with acute diarrhea, in 7 cities of argentina. diarrheagenic e. coli exhibited 74.5% of resistance against ampicillin, 64.2% against sulfametoxazole-trimethoprim, and shigella spp., 62% and 75.6% respectively. salmonella sp. showed 35%, 14%, 41.8%, 65.4%, 14.5%, ...199910932758
bacterial contamination of bottle milk in infants under 6 months in children's hospital, bangkok, thailand.the bacterial contamination of bottle milk samples obtained randomly from 500 infants under 6 months of age who came to the out-patient department of children's hospital bangkok was determined by collecting bottle milk samples prepared at home following interview of their caretakers after obtaining their consent. bacterial contamination was found in 91.8% (459/500) of bottle milk samples. among the positive samples, 82.8% (380/459) contained enteric bacteria, another 17.2% were unidentified bact ...199910928374
impact of an urban effluent on the bacterial community structure in the arga river (spain), with special reference to culturable gram-negative rods.the arga river is an interesting system in which to study the impact of urban effluent pollution because it receives a single effluent in the form of wastewater discharge from the city of pamplona. to analyze the extent of this discharge, total bacteria, culturable heterotrophic bacteria, and gram-negative heterotrophic bacteria were enumerated and 409 isolates of the latter were identified. one sampling station was located upstream from the inflow, while five were located up to 30 km downstream ...199910907419
diversity of culturable heterotrophic aerobic bacteria in pristine stream bed sediments.more than 900 culturable, heterotrophic aerobic isolates were obtained from the sediments of a forested, pristine stream and analyzed using three classical microbiological tests: api 20e, amplified ribosomal dna restriction analysis (ardra), and fatty acid analysis. gram-negative bacteria comprised most of the heterotrophic aerobic isolates (66.7%), similar to other oligotrophic environments. the isolates were assigned to the genus level as pseudomonas, flavobacterium, micrococcus, bacillus, chr ...199910907425
combined infection of norwalk-like virus and verotoxin-producing bacteria associated with a gastroenteritis outbreak.detection of multiple pathogens, particularly a combination of viruses and bacteria, is infrequently documented in outbreaks of gastroenteritis. this paper reports the presence of norwalk-like virus (nlv) and enterohaemorrhagic verotoxin-producing escherichia coli in one individual, and nlv and verotoxin-producing aeromonas sobria in another individual, both part of a large gastroenteritis outbreak. the causes of gastroenteritis in such outbreaks may be more complex than previously thought.199910892495
virulence patterns of aeromonas eucrenophila isolated from water and infected fish.six isolates of aeromonas eucrenophila--2 from water and 4 from superficial skin ulcer of cat fish--were examined for haemagglutination, serum sensitivity, chitinase production and enterotoxicity, and correlation, if any, between them; only one strain showed haemagglutination and was inhibited by both d-mannose and l-fucose. all the strains showed resistance to normal human serum, but produced chitinase; one of them elaborated inducible chitinase. all these strains caused fluid accumulation only ...199910892496
characterization of virulence factors of aeromonas isolated from children with and without diarrhoea in tripoli, libya.during september 1992-august 1993, stool samples from 157 children with diarrhoea and 157 matched healthy controls were examined for the presence of aeromonas and other enteropathogens. aeromonas strains were tested for haemolytic activity, haemagglutination patterns, and antibiotic susceptibility. in total, 62 aeromonas were isolated, of which 27 (17.2%) were from children with diarrhoea and 35 (22.3%) from healthy controls. only 23 (14.6%) of the diarrhoeal children and 28 (17.8%) of the healt ...199910897890
virulence factors in aeromonas veronii bv veronii. 199910897894
some emerging food and water borne pathogens.emerging pathogens are those infective organisms whose incidence has recently increased or is likely to increase during the next two decades due to changes in demography, food habits, food technology, commerce, water sources and environmental factors. some important emerging food and water borne bacterial pathogens include listeria monocytogenes, campylobacter jejuni, yersinia enterocolitica, salmonella enteritidis, escherichia coli o157: h7, vibrio cholerae biotype e1 tor serotype 0139, vibrio ...199910810592
[cellulitis caused by aeromonas hydrophila].we report the case of a cirrhotic patient with leukocytoclastic vasculitis who developed a rapid and progressive cellulitis with hemorrhagic bulla and sepsis due to aeromonas hydrophila, the portal of entry was the surgical leech of a cutaneous biopsy.199910686717
detection, prevalence, purification and characterization of lecithinase of klebsiella pneumoniae.lecithinase activity in klebsiella is a rare trait as out of 208 strains of klebsiella belonging to 3 species, viz. k. pneumoniae (168), k. planticola (29) and k. oxytoca (11), only 4 strains of k. pneumoniae produced lecithinase positive colonies on egg-yolk-agar. although cell lysates of 16 k. pneumoniae yielded positive results for lecithinase assay on egg-yolk-agar, 19 strains were detected positive for lecithinase with elisa using anti-lecithinase serum. release of up to 52.12% cell-bound l ...199910687289
[bacterial origin of acute diarrhea in merida, venezuela].464 stool specimens from children under 5 with acute diarrheal disease and other 149 specimens from the control group were studied from july, 1993, to may, 1995. the specimens were collected at the pediatric emergency department of the autonomous institute of the teaching hospital of los andes, mérida, venezuela. the presence of the internationally recommended bacterial, parasitary and viral agents was investigated. the commonest bacteria isolated as unique pathogens were: shigella (42.85%), shi ...199910887550
identification of aeromonas strains of clinical origin with atypical phenotypical profiles.a total of 47 strains of aereomonas isolated from patients with gastroenteritis was analyzed for 40 phenotypical characters and for evaluating the numeric taxonomy based on 27 discriminatory tests. it was proved that the clinical isolates showed a relative phenotypical distance and the groups of strains that had atypical profiles were compared with the type species by the present identification schemes.199910887556
numerical taxonomy of gram-negative, facultative anaerobic bacteria isolated from skin of turbot (scophthalmus maximus) and surrounding water.a numerical taxonomic study of 473 gram negative heterotrophic facultative anaerobic bacteria isolated from skin of turbot (scophthalmus maximus) and its culture water was performed. the study included 53 type and reference strains belonging to the genera vibrio, aeromonas and listonella. the strains were characterized using 90 tests and data were examined by simple matching coefficient (s(sm)) and jaccard coefficient (s(j)). upgma (unweighted pair group method, arithmetic average) defined 66 ph ...199910794149
in vitro susceptibilities of aeromonas genomic species to 69 antimicrobial agents.a total of 217 strains representing all 14 currently described genomic species in the genus aeromonas were tested for susceptibility to 69 antimicrobial agents by a microdilution method. all species were susceptible to tetracyclines, quinolones, chloramphenicol, and most of the aminoglycosides and the cephalosporins, but were resistant to lincosamides, vancomycin, teicoplanin and some penicillins. in general, no significant differences were found that correlated with the taxonomic designation or ...199910794154
site-directed mutagenesis of asp313, glu315, and asp391 residues in chitinase of aeromonas caviae.site-directed mutagenesis was used to explore the roles of amino acid residues involved in the activity of chitinase from aeromonas caviae. kinetic parameters for 4-methylumbelliferyl-n,n'-diacetyl-chitobiose or 4-methylumbelliferyl-n,n',n"-triacetylchitotriose hydrolysis were determined with wild-type and mutant chitinases. chitinases with the mutations e315d (or q) and d391e (or n) were severely impaired and had dramatically decreased kcat. however, the effect of the these mutations on the km ...199910794597
neem (azadirachta indica) extract as an antibacterial agent against fish pathogenic bacteria.aquaneem, an emulsified product prepared from the neem (a. indica) kernel was tested against four pathogenic bacteria of fish (i.e. aeromonas hydrophila, pseudomonas fluorescens, escherichia coli and myxobacteria spp.) to test its efficacy as an antibacterial agent. growth inhibitory property of the product at 10, 15 and 20 ppm has been noticed and recorded. the percentage reduction of bacterial cell population was noted to be maximum on 9th day at 20 ppm concentration (i.e. 70.14%, 74.15% and 6 ...199910783742
cytotoxicity of non o1, non o139 vibrios isolated from fresh water bodies in vellore, south india.the samples of plankton, soil sediment and water from a pond, a lake and a moat respectively in and around vellore were studied for environmental vibrios. vibrios were isolated from all these specimens after enrichment in alkaline peptone water and subculture on selective media. non o1, non o139 vibrio cholerae, aeromonas spp. and plesiomonas spp. were isolated. there were no isolates of v. cholerae serogroup o1 and o139. representative strains of non o1 and non o139 v. cholerae from environment ...199910680299
successful use of the medicinal leech (hirudo medicinalis) for the treatment of venous insufficiency in a replanted digit. 199910731346
quality of water--quality of life.especially in developing countries, the problem of adequate drinking water supply is an ever growing one. public health programmes have been established to improve the population's health conditions, but these programmes require big financial means for guaranteeing adequate supply of potable water and medical therapy for sick people. too little emphasis is still put on regular testing of drinking water for microorganisms such as aeromonas sp. and vibrio sp. in a spot check analysis in various co ...199910659386
amine borate catabolism by bacteria isolated from contaminated metal-working fluidsfour bacterial strains (tentatively identified as strains of aeromonas, pseudomonas, flavobacterium and bacillus) isolated from contaminated metal-working fluids were assayed for the capacity to utilize the borate derivatives of monoethanolamine (mea), diethanolamine (dea) and triethanolamine (tea). two of these strains, isolates av1 (flavobacterium) and cl1 (bacillus) were capable of growth on each of the borate esters with cell yields of 0.6 gl - 1 for av1 cultured on dea- and tea-borate, 0.3- ...199910594706
the extracellular regions of psma and the transferrin receptor contain an aminopeptidase domain: implications for drug design.the aeromonas proteolytica aminopeptidase (amp), pseudomonas sp. (rs-16) carboxypeptidase g2 (cpg2), and streptomyces griseus aminopeptidase (sgap) are zinc dependent proteolytic enzymes with cocatalytic zinc ion centers and a conserved aminopeptidase fold. a blast search with the sequence of the solved amp structure indicated that a similar domain could be found in prostate-specific membrane antigen (psma) and the transferrin receptor (tfr). when the psma or tfr sequence was input into the thre ...199910595564
aeromonas abscess in an immunocompromised child.an 11-year-old immunocompromised child developed cellulitis and abscess due to aeromonas hydrophila at the site of bone marrow aspiration after swimming in a freshwater lake. the patient required treatment with intravenous antibiotics and surgical debridement to eradicate the infection. both common and unusual organisms may complicate infections at the sites of percutaneous procedures.199910598672
aeromonas-associated diarrhoea in bangladeshi children: clinical and epidemiological characteristics.we studied the clinical and epidemiological features associated with aeromonas diarrhoea by a hospital survey of 7,398 children under 5 years of age presenting with diarrhoea. the data were actually based upon two cohorts from this survey, the majority of the data being identified from 405 (5.5%) in whom aeromonas was the sole enteric pathogen. aeromonas caviae was the most prevalent species, accounting for 32% (129/405) of all isolates. eighty-three per cent of children with aeromonas-associate ...199910605516
size distribution of bacterial and fungal bioaerosols in indoor air.the aim of this study was to determine the size distribution of bacteria and fungi occurring in the air of human dwellings. the concentration and size distribution of particulate aerosol, gram-positive mesophilic bacteria, gram-negative mesophilic bacteria and fungi were examined in 60 flats situated in the upper silesia conurbation, southern poland. the investigated flats comprised three quantitatively equal (20 flats each) groups: flats without additional emission sources of particulate aeroso ...199910607991
dimer dissociation of the pore-forming toxin aerolysin precedes receptor binding.the pore-forming toxin aerolysin is secreted by aeromonas hydrophila as an inactive precursor. based on chemical cross-linking and gel filtration, we show here that proaerolysin exists as a monomer at low concentrations but is dimeric above 0.1 mg/ml. at intermediate concentrations, monomers and dimers appeared to be in rapid equilibrium. all together our data indicate that, at low concentrations, the toxin is a monomer and that this species is competent for receptor binding. in contrast, a muta ...199910608828
recent developments in fish vaccinology.during the last 10 to 20 years vaccination has become established as an important method for prevention of infectious diseases in farmed fish, mainly salmonid species. so far, most commercial vaccines have been inactivated vaccines administered by injection or immersion. bacterial infections caused by gram-negative bacteria such as vibrio sp., aeromonas sp., and yersinia sp. have been effectively controlled by vaccination. with furunculosis, the success is attributed to the use of injectable vac ...199910614510
high mortality in zebrafish (danio rerio).a group of 100 adult zebrafish were housed in a new system at a stocking density of 20 fish per tank. four weeks after arrival, 15 fish presented with petechial hemorrhages and ulceration on the surfaces of the skin. samples of the fish were collected for histopathology, fungal culture, and bacterial culture and sensitivity. water samples were analyzed for ph, ammonia, nitrite, and submitted for bacterial and fungal culture. histologically, the epidermis had multiple areas of ulceration and mono ...199912086431
cloning and nucleotide sequencing of prolyl endopeptidase gene from aeromonas punctata subsp. punctata.prolyl endopeptidase activity was found in aeromonas punctata subsp. punctata. the genomic dna was partially digested with ecori and the recovered 8-16 kb dna fragments were inserted into the ecori site of plasmid puc19, and were transformated into escherichia coli dh5alpha. the resulted clones were screened by using benzyloxycarbonyl-gly-pro-beta-naphthylamide, the specific substrate of prolyl endopeptidase and a positive clone was obtained. the 12 kb insertion fragment of recombinant plasmid w ...199912114972
properties of recombinant aeromonas punctata prolyl endopeptidase.the properties of recombinant aeromonas punctata prolyl endopeptidase(appep) were studied using specific substrate and peptides. results show that the optimum catalytic temperature and ph was 34 degrees and 8.4, the stability of the appep was in the range of 4-32 degrees and ph 6.0-10.0, and its k(m) was 0.03 mmol/l based on the z-gly-pro-betana. the appep was not sensitive to pmsf, tlck, tpck, trypsion inhibitor, edta, tetrathionate and some metal ions, but was sensitive to sds and zn(2 ), and ...199912110936
a comparison of bacillus cereus and aeromonas hydrophilia zn-beta-lactamases. 199915263322
bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria.on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ...199814666747
the development of random dna probes specific for aeromonas salmonicida.rapd-pcr has been used to produce dna probes for aeromonas salmonicida. dna hybridization studies showed that rapd-pcr fragments of the same size did not necessarily hybridize to each other and therefore these sequences were not always homologous. however, a single rapd-pcr fragment (designated 15e) was identified as being common to aer. salmonicida. subsequently, 15e was found to comprise five dna fragments of similar size which differed in their nucleotide sequences. all five fragments were ev ...199815244055
the acetylcholinesterase ichthyotoxin is a common component in the extracellular products of vibrionaceae strains.in previous work, it was reported that a strain of aeromonas hydrophila (b32) produces the most potent lethal toxin with neurotoxic activity described so far for fish. in the present study, the presence and distribution of this acetylcholinesterase toxin lethal for fish were determined in extracellular products (ecp) of 42 vibrionaceae strains using both immunological and colorimetric methods. this neurotoxin was shown to be present in the majority of the ecp from the aeromonas and vibrio strain ...199815244056
specific probiotic characterization of weissella hellenica ds-12 isolated from flounder intestine.a total of 199 microorganisms were isolated from the intestinal contents of flounder (paralichthys olivaceus) in a fish farm in seoul, korea. among these strains, ds-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% nacl, 10% bile, and in broth at ph 3 for 90 min. this strain was gram-positive, and catalase-negative coccoid ...199812501410
lipase activity and gene cloning of acinetobacter calcoaceticus lp009.the production of lipase from acinetobacter calcoaceticus lp009, a bacterium isolated from raw milk, was found to be best induced by tween-80 at 1.0% concentration. it was efficiently secreted, and only a minute amount of activity was detected at the cell surface and intracellularly. a. calcoaceticus lp009 lipase exhibited maximum activity at ph 7.0 and 50 degrees c, and was relatively stable upon storage at ph 5.0 to 7.0 and at 4, 30, or 37 degrees c. the enzyme was found to be inactivated by e ...199812501281
identification of bacteria in water for pharmaceutical use.different systems for the obtention of water used in biopharmaceutical industry were characterized from the bacteriological point of view. determination of aerobic mesophilic microorganisms was performed; as well as the isolation of contaminant microorganisms for what the techniques of membrane filtration was used. for the identification of the more representative species there were made conventional biochemical tests and quick systems: api. the results show that water serving as tap water for p ...199810932743
[development of an elisa-system for the assessment of furunculosis vaccines]as an alternative method for challenge experiments in fish a sandwich-elisa has been established for the detection of potentially protective antibody populations. assay specificity depends on monoclonal antibodies (mabs) directed against different antigens of aeromonas salmonicida, the causative agent of furunculosis. comparable levels of salmon antibodies against lps and the a-layer protein were recorded by elisa and antigen binding assay. these studies, together with the first analysis of trou ...199811178550
a case of acute cholecystitis due to aeromonas sobria and hafnia alvei from northern europe. 199811864254
coordination chemistry of the amonabactins, bis(catecholate) siderophores from aeromonas hydrophila(1).the amonabactins are a series of four bis(catecholate) siderophores isolated from the pathogenic organism, aeromonas hydrophila. as tetradentate ligands, they cannot singly satisfy the octahedral coordination sphere of iron. the solution coordination chemistry of the amonabactins has been elucidated using potentiometric and spectrophotometric titrations, circular dichroism, and mass spectroscopy. they form 2:3 metal:ligand complexes at high ph and excess ligand. their complexation behavior is es ...199811670604
an attempt to use selected strains of bacteria adapted to high concentrations of petroleum oil to increase the effective removal of petroleum products in excess activated sludge in laboratory conditions.forty two strains of bacteria were isolated from excess activated sludge from petroleum wastewater treatment plant. the strains were identified and classified to the following groups: enterobacteriaceae (7 strains), anitratum (3 strains), pseudomonas (13 strains), micrococcus (12 strains), comamonas (2 strains), xanthomonas (2 strains), achromobacter (1 strain) and vibrio-aeromonas (1 strain). one of the isolates was found to be a yeast strain. following preliminary selection ten strains, showin ...19989990713
isolation of aeromonas salmonicida in association with purple-pigmented bacteria in sediment from a scottish loch.aeromonas salmonicida was recovered in close association with an unidentified purple-pigmented organism, which was isolated from sediment in a scottish loch during november (1997) and february (1998). however, there has not been any evidence of a. salmonicida infections, specifically furunculosis, associated with the fish in this loch.19989871353
distributional patterns of bacterial diarrhoeagenic agents and antibiograms of isolates from diarrhoeaic and non-diarrhoeaic patients in urban and rural areas of nigeria.to determine the prevalence of bacteria that could cause diarrhoea in stool specimens of individuals with and without diarrhoea in both urban and rural areas of nigeria. to ascertain the antibiotic susceptibilities of the bacterial diarrhoeagenic agents isolated. to document the predominant signs and symptoms associated with the various bacterial agents of diarrhoea.199810101427
ulcerative dermatitis caused by aeromonas salmonicida spp. salmonicida in farmed atlantic salmon in british columbia. 199810051960
the production of a novel carbapenem-hydrolysing beta-lactamase in aeromonas veronii biovar sobria, and its association with imipenem resistance. 199810052912
resistance to antibiotic and heavy metals of motile aeromonads from chilean freshwater.in this work the resistance of 172 motile aeromonas isolates recovered from raw drinking water supplies (56), irrigation waters (60) and runoff waters receiving sewage (56), to some antibiotics and heavy metals was investigated by agar diffusion and agar dilution methods. a high proportion of isolates from all water sources showed resistance to carbenicillin, erythromycin, streptomycin, cephradine and cadmium, and susceptibility to chloramphenicol, kanamycin, gentamicin, tetracycline, nalidixic ...19989926432
small bowel and fecal microbiology in children suffering from persistent diarrhea in bangladesh.the etiology of persistent diarrhea in children is multifactorial. the objective of the current study was to ascertain the role of microorganisms in the etiology and pathogenesis of persistent diarrhea in a group of children in bangladesh.19989443113
pharmacokinetics of oxytetracycline in the red pacu (colossoma brachypomum) following different routes of administration.oxytetracycline (otc) pharmacokinetics were studied in the red pacu (colossoma brachypomum) following intravenous (i.v.) and intramuscular (i.m.) administration at a dose of 5 mg/kg body weight. otc plasma concentrations were determined by high-performance-liquid-chromatography (hplc). a non-compartmental model was used to describe plasma drug disposition after otc administration. following i.m. administration, the elimination half-life (t1/2) was 62.65 +/- 1.25 h and the bioavailability was 49. ...19989811436
population dynamics of aeromonas spp. in an urban river watershed.density of aeromonas spp. at one site in the buffalo river and at four sites on its upstream tributaries was followed from june 1992-june 1993. membrane filtration counts of aeromonas during the summer ranged between 18 and 4000 ml-1, which were one to two logs higher than faecal coliform and faecal streptococci densities. aeromonas spp. in the buffalo river, and faecal coliforms, faecal streptococci, and the heterotrophic plate count throughout the watershed, increased by approximately one log ...19989812384
inhibition of streptomyces griseus aminopeptidase and effects of calcium ions on catalysis and binding--comparisons with the homologous enzyme aeromonas proteolytica aminopeptidase.streptomyces griseus aminopeptidase is a zinc metalloenzyme containing 2 mol zinc/mol protein, similar to the homologous enzyme aeromonas proteolytica aminopeptidase. in addition, a unique ca2+-binding site has been identified in the streptomyces enzyme, which is absent in the aeromonas enzyme. binding of ca2+ enhances stability of the streptomyces enzyme and modulates its activity and affinity towards substrates and inhibitors in a structure-dependent manner. among the three hydrophobic 4-nitro ...19989874195
a collagen-binding protein produced by aeromonas hydrophila.the gastrointestinal pathogen aeromonas hydrophila strain a186 produces a collagen-binding protein (cnbp) which is found extracellularly and loosely associated with the cell surface. the cell-associated cnbp was purified by sequential ammonium sulphate precipitation, size-exclusion chromatography and ion-exchange chromatography, or by sequential ammonium sulphate precipitation and affinity chromatography with collagen-sepharose. the purified cnbp was homogeneous in sds-page, and had a mol. wt of ...19989879942
a cluster of cases of infections due to aeromonas hydrophila revealed by combined rapd and eric-pcr.two polymerase chain reaction (pcr)-based methods were used for epidemiological typing of aeromonas hydrophila. random amplification of polymorphic dna (rapd) and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) were applied to an outbreak involving seven patients. the epidemiological situation appeared complex; with the exception of two clinical isolates, all gave unique patterns with both techniques. these methods demonstrated nosocomial transmission in one unit and permitted the ...19989879968
characterisation of a type iv pilus produced by aeromonas caviae.a pilus produced by a clinical isolate of aeromonas caviae (strain ca195) was purified and partially characterised. the mr of the pilin was estimated to be 23 kda by sds-page. its n-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type iv pili purified from other aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. bundle-forming pili, often with a polar location, were seen on the surface of strain ca19 ...19989879972
virulence factors of aeromonads--an emerging food borne pathogen problem.aeromonas spp. possess a number of virulence properties which are considered responsible for intestinal and extra-intestinal infections in human beings and also for a wide variety of infections in animals. the paper discusses current status of colonization and toxic factors of aeromonas spp., especially in relation to food and biochemical markers. future research needs are also identified.19989914671
aeromonas hydrophila associated with a severe outbreak of infection in farmed rabbits.an outbreak of infection with a high rate of mortality has been detected in an industrial rabbitry in spain. aeromonas hydrophila was isolated in pure culture from liver, lung, heart and spleen of ill and moribund animals. all the isolates belonged to o:11 serogroup, autogglutinated after boiling, were resistant to the bactericidal action of the fresh rabbit serum and did not agglutinate in acriflavine. they were producers of hemolysins and proteases but were not enterotoxigenic.19989916296
[meningitis by aeromonas sobria following cranio-encephalic traumatism]. 19989919002
construction of a physical and preliminary genetic map of aeromonas hydrophila jmp636.a physical and preliminary genetic map of the aeromonas hydrophila jmp636 chromosome has been constructed. the topology of the genome was predicted to be circular as chromosomal dna did not migrate from the origin during pfge unless linearized by s1 nuclease. cleavage of the chromosome with paci and pmei produced 23 and 14 fragments, respectively, and enabled calculation of the genome size at 4.5 mb. digestion of the chromosome with i-ceui produced 10 fragments, indicating that 10 rrl (23s) gene ...19989846744
mobile drug resistance genes among fish bacteria. 19989850686
transcriptional regulation and organization of the dcua and dcub genes, encoding homologous anaerobic c4-dicarboxylate transporters in escherichia coli.the dcua and dcub genes of escherichia coli encode homologous proteins that appear to function as independent and mutually redundant c4-dicarboxylate transporters during anaerobiosis. the dcua gene is 117 bp downstream of, and has the same polarity as, the aspartase gene (aspa), while dcub is 77 bp upstream of, and has the same polarity as, the anaerobic fumarase gene (fumb). to learn more about the respective roles of the dcu genes, the environmental and regulatory factors influencing their exp ...19989852003
[bacteriological quality of spring and well water in yaounde (cameroon)].the drinking water supply in yaounde, cameroon, often fails to meet demand and this leads many people to seek water from springs and wells the microbiological quality of which is unknown. we carried out a microbiological survey of five spring water points and ten wells. the fifteen study sites were located in various residential districts and were selected according to geographical location and number of users. microorganisms were isolated on specific culture media using the membrane filter tech ...19989854008
optimisation of the protocol for detection of aeromonas species in ready-to-eat salads, and its use to speciate isolates and establish their prevalence.aeromonas spp. are detected in more than 500 cases of gastrointestinal infection each year in england and wales. this study aimed to identify their prevalence in ready-to-eat salads, which are a potential source of aeromonas infection. the protocol for isolation of mesophilic aeromonas spp. from salads was optimised. using the improved method, aeromonas spp were isolated from 19 of 25 samples (25 g) of ready-to-eat salad products. aeromonas organisms were counted, isolates were identified to spe ...19989854886
the risk to public health of aeromonas in ready-to-eat salad products.mesophilic aeromonas spp. are isolated regularly from cases of gastrointestinal infection and have markers indicative of enteropathogenicity. is aeromonas, which is present in a large proportion of ready-to-eat salads, actually a gastrointestinal pathogen? isolates of mesophilic aeromonas from salads were characterised in terms of their ability to grow at refrigeration temperatures over the given shelf life and by the presence of markers of potential virulence. the major phenospecies present in ...19989854887
purification and characterization of enterotoxin produced by aeromonas sobria.we purified the toxin of aeromonas sobria capable of inducing a positive response in the mouse intestinal loop assay. the purified toxin showed a positive response not only in the loop assay but also in a hemolytic assay. subsequently, we cloned the toxin gene and demonstrated that the product of this gene possessed both hemolytic and enterotoxic activities. these results showed that the enterotoxin of a. sobria possesses hemolytic activity. nucleotide sequence determination of the toxin gene an ...19989858466
the acute phase response in the sudan plated lizard, gerrhosaurus major.the sudan plated lizard (gerrhosaurus major), previously reported to be an afebrile species, was utilized in a series of experiments to test for various aspects of the acute phase response. treatment of individuals with the antibiotic baytril resulted in a slight (0.5 degree c) but significant reduction in mean selected body temperature (msbt), while treatment with saline did not lower msbt. nonantibiotic treatment individuals had depressed plasma iron levels (86.6 +/- 22.4 micrograms fe 100 ml- ...19989773512
inducible beta-lactam resistance in aeromonas hydrophila: therapeutic challenge for antimicrobial therapy.despite the abundant amount of knowledge about inducible chromosomally mediated beta-lactamases among aeromonas species, extended-spectrum beta-lactam-resistant a. hydrophila strains selected in clinical practice were rarely reported. in the present study, two strains of a. hydrophila, a136 and a139, with markedly different susceptibilities to extended-spectrum cephalosporins were isolated from blood and the tip segment of an arterial catheter of a burn patient. another strain (a136m) was select ...19989774563
haemorrhagic septicaemia by aeromonas salmonicida subsp. salmonicida in a black-tip reef shark (carcharhinus melanopterus).one black-tip reef shark (carcharhinus melanopterus) was found dead without previous signs of disease. major lesions consisted in cutaneous erythema, mainly at the base of the fins, focal to diffuse inflammatory lesions in gills and intestinal wall, and discrete haemorrhages in the same organs, liver and kidneys. microcolonies of gram-negative rods were observed in the lamina propia of gills, underneath the intestinal mucosa and randomly distributed in the renal and hepatic parenchymas. also, em ...19989780832
bacteriophage pm2 nomenclature revision. 19989787669
plasma proteins of rainbow trout (oncorhynchus mykiss) isolated by binding to lipopolysaccharide from aeromonas salmonicida.in an attempt to find plasma proteins that might be involved in the constitutive resistance of rainbow trout to furunculosis, a disease caused by aeromonas salmonicida (as), we purified serum and plasma proteins based on their calcium- and carbohydrate-dependent affinity for a. salmonicida lipopolysaccharide (lps) coupled to an epoxy-activated synthetic matrix (toyopearl af epoxy 650m). a multimeric family of high molecular weight (96 to 200-kda) lps-binding proteins exhibiting both calcium and ...19989787816
experimental infections of rana esculenta with aeromonas hydrophila: a molecular mechanism for the control of the normal flora.frogs can be useful models for studying the mechanisms that may regulate their natural microbial flora. their skin glands produce a secretion containing 20-30 different peptides, some antimicrobial some neurotrophic. as they often live in soil or silt that is rich in microbes, they can be expected to be able to prevent or eliminate infections in very short periods of time. the bacterium aeromonas hydrophila is widely distributed in nature and is considered as part of the natural flora of frogs a ...19989790305
endochitinase is transported to the extracellular milieu by the eps-encoded general secretory pathway of vibrio cholerae.the chia gene of vibrio cholerae encodes a polypeptide which degrades chitin, a homopolymer of n-acetylglucosamine (glcnac) found in cell walls of fungi and in the integuments of insects and crustaceans. chia has a coding capacity corresponding to a polypeptide of 846 amino acids having a predicted molecular mass of 88.7 kda. a 52-bp region with promoter activity was found immediately upstream of the chia open reading frame. insertional inactivation of the chromosomal copy of the gene confirmed ...19989791107
secretion and properties of the large and small lobes of the channel-forming toxin aerolysin.aerolysin is a dimeric protein secreted by aeromonas spp. that binds to glycosylphosphatidylinositol-anchored receptors on target cells and becomes insertion competent by oligomerizing. the protein comprises two lobes joined by a short arm. the large lobe is thought to be responsible for channel formation, whereas the small lobe is believed to stabilize the dimer, and it may also contain the receptor binding site. we cloned and expressed the dna for both lobes of the toxin separately and togethe ...19989791179
occurrence of heterotrophic bacteria and fungi in an aviation fuel handling system and its relationship with fuel fouling.clean, dry and contaminant-free fuel is necessary for safe and economical aircraft operation. microbial growth in aviation fuel handling systems can alter the quality of the product. this paper reports the occurrence of heterotrophic bacteria and fungi in a handling system of jet a-1 aviation turbine fuel. a total of 350 samples were collected during 1990-1996. the aerobic microorganisms in fuel samples were mainly fungi, 85% of samples containing < or = 100 cfu/l (range 0 (< 1 cfu/l) to 2000 cf ...19989793139
molecular characterization of plasmid-mediated oxytetracycline resistance in aeromonas salmonicida.using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (ot)-resistant isolates of aeromonas salmonicida transferred the ot resistance phenotype to escherichia coli. the ot resistance phenotype was encoded by high-molecular-weight r-plasmids that were capable of transferring ot resistance to both environmental and clinical isolates of aeromonas spp. the molecular basis for antibiotic resistance in ot-resistant isolates of a. salmonicida was determined. the ot resistance determinant ...19989797265
adhesion of aeromonas hydrophila to water distribution system pipes after different contact times.scanning electron microscopy observation was used to investigate the ability of aeromonas hydrophila to attach to various water distribution pipe surfaces, such as stainless steel, copper, and polybutylene, after different contact times at ambient and storage temperatures. surface energy value of each surface was estimated by contact angle measurements using water, alpha-bromonaphthalene, and dimethyl sulfoxide. our results indicated that aeromonas cells could easily attach to all surface types ...19989798149
susceptibility of antibiotic-resistant and antibiotic-sensitive foodborne pathogens to acid anionic sanitizers.acid anionic sanitizers for treatment of fruits and vegetables were prepared using ingredients generally recognized as safe by the u.s. food and drug administration or anionic surfactants and organic acid food additives. they met the regulatory definition as sanitizers by showing bactericidal efficacy of 99.999% in 30 s against staphylococcus aureus atcc 6538 and escherichia coli atcc 11229. these sanitizers showed a broad spectrum of microbicidal activity against both gram-positive and gram-neg ...19989798163
sensitivity pattern and beta-lactamase production in clinical isolates of aeromonas strains.of the 43 aeromonas spp. isolated from various clinical samples 94% isolates were beta-lactamase producers. the isolates were tested for sensitivity by disc diffusion method which is commonly used in pakistan. mic was determined by using epsilometer test (e-test) method. more than 80% isolates were sensitive to cephalosporins and quinolones. however, resistance to commonly used antibiotics was very high, 94% isolates were resistant to ampicillin which corresponds to the betalactamase production. ...19989813984
[sanitary and microbiological assessment of marketable fish of the volga-caspian sea region].opportunistic microbes, such as aeromonads, enterobacteria, and pseudomonads, which are responsible for spoiling fish during its storage and processing and capable to initiate pathological processes of fishes are common in the volga-caspian sea region. investigations demonstrated that it is necessary to develop new procedures for processing fish products, to carry out studies on regulation of microbiological criteria for assessing fish quality. this makes it necessary to make a bacteriological c ...19989816781
[factors of persistence and (or) pathogenicity in vibrios and aeromonads belonging to different ecotopes].factors of persistence and/or pathogenicity in vibrio parahaemolyticus and aeromonas hydrophila (hemolytic, lipase, lecithin, dnaase, rnaase, antilysozyme, "anti-interferon", anticomplementary activities and capacity for absorbing congo red) were studied. the study revealed the interspecific and subpopulation (hospital and extraorganismal parts of the population) differences in the activity of the manifestation of these factors. strong dependence of the whole complex of persistence and pathogeni ...19989825495
[database on nucleotide sequences used as primers of microorganisms].the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ...19989825498
brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes.brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. in this study, we have examined the intracellular pathway of the virulent b. abortus strain 2308 (s2308) and the attenuated strain 19 (s19) in hela cells. at 10 min after inoculation, both bacterial strains are transiently detected in phagosomes characterized by the presence of early endosomal markers such as the early endosomal antigen 1. at approximately 1 h postinoculation, bacteria are locate ...19989826346
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