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differential susceptibility of aeromonads and coliforms to cefsulodin.volumn 62, no. 6, p. 1885, abstract, line 3: "for 102 of 129 aeromonas isolates" should read "for 102 of 130 aeromonas isolates." page 1887, column 1, lines 16 and 17: "and for 13 of 21 coliform isolates" should read "and for 13 of 94 coliform isolates." [this corrects the article on p. 1885 in vol. 62.].199616535434
chronic colitis after aeromonas hydrophilia infection. 199617429262
[aeromonas hydrophila pneumonia associated with a traffic accident. report of a case].the case of a patient who was driving a car after getting drunk is presented. his car turned over and he fell into an irrigation canal, and, as a result, he suffered from an incomplete drowning syndrome. he was admitted in the intensive care unit with acute inflammatory pneumonia and a strain of aeromonas hydrophila was isolated in blood. the patient's evolution was favorable. it is the first report on a case like this in our country.19969768270
survey on the distribution of vibrionaceae at the seaport areas in taiwan, 1991-1994.a monthly survey on the distribution of human-pathogenic vibrionaceae of the seawater from five principal harbors in taiwan was conducted by national quarantine service from july, 1991 to february, 1994. of the total 1,167 vibrionaceae isolates, strains of vibrio alginolyticus (449 strains) were the most frequently isolated, followed by vibrio parahaemolyticus (262) , aeromonas hydrophila (153), vibrio cholerae non-o1 (86), and vibrio vulnificus (67). none of vibrio cholerae o1 was isolated. the ...199610592802
in-vitro acquisition of iron from the iron---desferrioxamine complex by aeromonas hydrophila. 199611866778
detección de escherichia coli toxigénica (lt) mediante la reacción en cadena de la polimerasa [the detection of toxigenic escherichia coli (lt) by the polymerase chain reaction].in this paper it is described the detection enteroxigenic escherichia coli lt (+). this method is based on the amplification of a dna fragment of 400 pairs of bases by polymerase chain reaction (prc). the oligonucleotides were designed by the authors and the characteristic patterns were observed when the samples were submitted to an electrophoresis in an agarose gel at 2%. the pcr had positive results with the strains of escherichia coli 0:149 k; 88 (lt+) collection and with 20 strains isolated ...19969805043
breast feeding may protect children against campylobacter jejuni associated diarrhoea.to investigate the bacterial etiology of infant diarrhea in karachi, pakistan, 180 rectal swab samples collected from diarrhea patients under 5 years of age and 30 samples from age- and sex-matched healthy children were analyzed. enteropathogenic organisms were isolated from 70 case samples (38.9%). the two most common etiologic agents were campylobacter jejuni (19.4%) and aeromonas hydrophilia (6.1%). salmonella, shigella, and yersina appear to have a limited incidence in this age group. no ...199612346517
cloning of a cluster of chitinase genes from aeromonas sp. no. 10s-24.a gene encoding chitinases from aeromonas sp. no. 10s-24 was cloned into escherichia coli dh5 alpha using puc19, and its nucleotides were sequenced. the chitinase gene was clustered in orfs (open reading frame) 1 to 4, in a 8-kb fragment of dna. orf-1 consisted of 1608 bp encoding 535 amino acid residues, and orf-2 consisted of 1425 bp encoding 474 amino acid residues. orf-3 was 1617 bp long and encodes a protein consisting of 538 amino acids. orf-4 encodes 287 amino acids of the n-terminal regi ...19968605248
identification of the catalytic triad of the lipase/acyltransferase from aeromonas hydrophila.aeromonas hydrophila secretes a lipolytic enzyme that has several properties in common with the mammalian enzyme lecithin-cholesterol acyltransferase. we have recently shown that it is a member of a newly described group of proteins that contain five similar blocks of sequence arranged in the same order in their primary structures (c. upton and j. t. buckley, trends biochem. sci. 233:178-179, 1995). assuming that, like other lipases, these enzymes have a ser-asp-his catalytic triad, we used thes ...19968606184
growth and exotoxin production at low temperatures by aeromonas strains in meat slurries.the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (haemolysin and enterotoxin) and exoenzymes (protease and lipase), as well as their growth in meat slurries stored at low temperatures (5 and 12 degrees c) was investigated. the results showed that aeromonas grew well in meat slurries stored at low temperatures in the presence of background flora. exotoxins and exoenzymes were not detected in filtrates from meat slurries inoculated with enter ...19968643030
the structure of the aeromonas proteolytica aminopeptidase complexed with a hydroxamate inhibitor. involvement in catalysis of glu151 and two zinc ions of the co-catalytic unit.the structure of the complex of aeromonas proteolytica aminopeptidase, a two-zinc exopeptidase, with the inhibitor p-iodo-d-phenylalanine hydroxamate has been determined by x-ray crystallography. refinement of the structure, which includes 220 water molecules, using data at 0.80-0.23-nm resolution resulted in a crystallographic residual r value of 16%. the hydroxamate group adopts a planar conformation whereby the two oxygen atoms interact with the zinc ions. the n-hydroxyl group of the inhibito ...19968647077
the 'hidden' carbapenemase of aeromonas hydrophila.it has been presumed that there are just two beta-lactamases in the motile aeromonas species, a carbapenemase and a cephalosporinase, based on the premise that all beta-lactamases can be detected by hydrolysis of the chromogenic cephalosporin, nitrocefin. however, when it was recently found that a non-motile species of aeromonas that causes furunculosis in salmon, contained three beta-lactamases, one of which was a carbapenemase which could not be detected with nitrocefin, it was hypothesised th ...19968647771
survival and growth of aeromonas hydrophila on modified atmosphere packaged normal and high ph lamb.the growth of a. hydrophila on normal ph (5.5-5.8) and high ph ( > 6.0) lamb stored under modified atmospheres was examined. lamb pieces and mince, inoculated with a. hydrophila were packaged in air, vacuum pack, 80% o2/20% co2, 50% co2/50% n2, or 100% co2 and stored at 5 or 0 degrees c for up to 42 days. samples were examined for the survival and/or growth of a. hydrophila by enrichment in alkaline peptone water and/or direct plating on starch ampicillin agar. the ph of each sample was estimate ...19968652346
gastroenteritis caused by aeromonas trota in a child.a case of acute diarrhoea caused by aeromonas trota (formerly hg 13 group) in a spanish child is reported. the strain was isolated in the faeces using the cin agar (cefsulodin-irgasan-novobiocin) culture media. the strain was initially identified as a sobria by the commercial gni card and api 20e biochemical systems. the strain was, however, vogesproskauer and sucrose negative, so complementary tests of cellobiose fermentation and gluconate oxidation were performed. these tests, together with th ...19968655689
characterization of aeromonas hydrophila strains of clinical, animal, and environmental origin expressing the o:34 antigen.a collection of aeromonas strains of different origins were characterized for isolates expressing the o:34 somatic antigen. of over 200 strains tested, approximately 14% belonged to serogroup o:34 with >85% of these strains identified as a. hydrophila regardless of source. a subset of 14 a. hydrophila o:34 strains were further analyzed for a number of structural and pathogenic features. most o:34 strains expressed similar whole-cell protein profiles with regards to minor bands, but major band di ...19968662181
aminopeptidase from streptomyces griseus: primary structure and comparison with other zinc-containing aminopeptidases.the aminopeptidase from streptomyces griseus is a calcium-activated metalloenzyme, which contains 2 mol tightly bound zinc/mol protein. this aminopeptidase rapidly hydrolyzes peptide bonds formed by n-terminal hydrophobic amino acids, such as leucine, methionine and phenylalanine. we have determined the complete primary structure of the protein, which contains 284 amino acid residues, yielding a molecular mass of 29723 da. a search in the swiss-prot database for sequence similarities revealed a ...19968665903
structure of the o-specific polysaccharide of an aeromonas trota strain cross-reactive with vibrio cholerae o139 bengal.the o-specific polysaccharide of an aeromonas trota strain was isolated by hydrolysis of the lipopolysaccharide at ph 4.5 followed by gel-permeation chromatography and found to consist of hexasaccharide repeating units containing d-galactose, l-rhamnose, 3,6-dideoxy-l-xylo-hexose (colitose, col), 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galactose in the ratios 1:1:2:1:1. partial hydrolysis of the polysaccharide with 48% hydrofluoric acid resulted in selective removal of colitose t ...19968665933
the o:34-antigen lipopolysaccharide as an adhesin in aeromonas hydrophila.we compared the ability of different aeromonas hydrophila strains from serogroup o:34 grown at different temperatures to adhere to hep-2 cells. we found a high level of adhesion when the strains were grown at 20 degrees c but not when they were grown at 37 degrees c. we previously described that these strains were able to form the o-antigen lipopolysaccharide when they grow at low temperature but not at high temperature. we also obtained by transposon mutagenesis mutants only devoid of the o-ant ...19968674995
endotoxin in aeromonas sobria infection: speculative pathogenesis and treatment strategies in advanced liver disease with soft-tissue infection. 19968678032
infections in the cirrhotic patient. 19968678033
[bacteremic peritonitis caused by aeromonas hydrophila in a woman with cirrhosis]. 19968679853
cloning, sequencing, and characterization of the nuch gene encoding an extracellular nuclease from aeromonas hydrophila jmp636.an escherichia coli clone expressing activity on dnase agar was obtained by cloning chromosomal dna of aeromonas hydrophila jmp636 into plasmid puc19. examination (of the clone's nuclease activity on a sodium dodecyl sulfate (sds)-polyacrylamide gel containing dna as a substrate revealed an activity band at approximately 100 kda. subsequently, subcloning localized the gene, designated nuch, to a 3.6-kb dna fragment (pjp9521). southern blotting of the nuch gene against chromosomal dna of jmp636 c ...19968682799
a multiplicity of bowel problems.a 55-year-old man presented with a 12-pound weight loss and acute diarrhea. he had had three watery stools a day for 12 days, as well as fever, chills, abdominal pain, nausea, and vomiting. he had not had any bowel movements at night, but noted that the first one each morning was tinged with blood.19968682885
immunotoxic effects of copper and cadmium in the sea bass dicentrarchus labrax.two phagocytes-mediated activities of the sea bass dicentrarchus labrax were examined after exposure to sublethal concentrations of copper and cadmium: (a) phagocytosis (measured by phagocytotic index), and (b) the production of reactive oxygen intermediates (luminol-dependent chemiluminescence) in response to bacteria aeromonas salmonicida. in vivo exposure for 48 h to each metal separately by intraperitoneal injection did not affect the quantity of phagocytes of pronephros and their viability ...19968683034
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
characterization of atypical aeromonas salmonicida isolates by ribotyping and plasmid profiling.a total of 38 strains of atypical aeromonas salmonicida, three oxidase-negative but otherwise typical aer. salmonicida, three typical aer. salmonicida, and two reference strains, isolated from several countries and fish species were examined with respect to rrna gene restriction patterns (ribotypes) and plasmid profiles. most epidemiologically unrelated strains had different ribotypes, whereas isolates from the same outbreak were identical. all strains, except one, carried one or more large plas ...19968698652
bacterial aminopeptidases: properties and functions.aminopeptidases are exopeptidases that selectively release n-terminal amino acid residues from polypeptides and proteins. bacteria display several aminopeptidasic activities which may be localised in the cytoplasm, on membranes, associated with the cell envelope or secreted into the extracellular media. studies on the bacterial aminopeptide system have been carried out over the past three decades and are significant in fundamental and biotechnological domains. at present, about one hundred bacte ...19968703509
utilization of transferrin and salmon serum as sources of iron by typical and atypical strains of aeromonas salmonicida.the ability of typical and atypical strains of aeromonas salmonicida to utilize non-haem sources of protein-bound iron was evaluated. (i) in a plate bioassay, the suppression of growth imposed on typical and atypical a. salmonicida by addition of the high-affinity iron chelator ethylenediamine-di(o- hydroxyphenylacetic acid) (edda) to the growth medium was reversed by the addition of 30% or 90% iron-saturated bovine or human transferrin (tf) or lactoferrin (lf) to the growth medium. (ii) the mec ...19968704995
microbiological quality & incidence of organisms of public health importance in food & water in ludhiana.bacteriological analysis of 713 samples of various types of foods and related articles and potable water samples from different places in ludhiana, punjab was carried out. the highest counts ranging from 2.5 x 10(6)-7.5 x 10(8) organisms/g were observed in raw vegetables and fruits, followed by 3 x 10(6)-9.8 x 10(7)/ml, 8.3 x 10(4)-8.9 x 10(7)/g and 1 x 10(3)-6.7 x 10(7)/g in fruit juice, milk and its products, and salty/non milk snacks respectively. fresh chapati, dal, rice, cooked vegetables a ...19968707360
colonization of streptomycin-treated mice by aeromonas species.streptomycin-treated adult mice were investigated as a possible model for studying the enteropathogenicity of aeromonas species. c57bl mice pre-treated with streptomycin (5.0 g/l drinking water, 48 hours) received a single intragastric dose (10(10) bacteria /10.5 ml) of one of six well-characterized, toxin-producing, human diarrhoeal isolates of a. veronii biovar sobria (n = 3) or a. hydrophila (n = 3). their faeces were examined for aeromonas for 10 days post-challenge. all strains colonized th ...19968708329
combined effect of gamma-irradiation and conventional cooking on aeromonas hydrophila in meatball.irradiation combined with a conventional cooking procedure was applied to meatball and the effects on bacterial load and inoculated aeromonas hydrophila were determined. meatball samples were irradiated by using a 60co source at the dose levels of 0, 0.30, 0.75, 1.50, 2.50 kgy and cold stored at 4 +/- 1 degrees c for 7 days. bacterial load and the count of a. hydrophila decreased when the irradiation dose level increased. a minimum inhibition effect was found at the dose of 0.30 kgy. irradiation ...19968717095
aeromonas bacteraemia in burn patients.human aeromonas infection is uncommon and is usually associated with immunosuppression, chronic disease or trauma in an aquatic setting. burn injury may induce a state of immunosuppression, making the thermally injured patient a suitable host for aeromonas infection. we reviewed the experience of one burn centre with this pathogen. retrospective examination of blood culture results from 8151 patients admitted between 1959 and 1994 disclosed eight patients with clinically relevant aeromonas hydro ...19968719317
characterisation of the heptameric pore-forming complex of the aeromonas toxin aerolysin using maldi-tof mass spectrometry.aerolysin, a virulence factor secreted by aeromonas hydrophila, is representative of a group of beta-sheet toxins that must form stable homooligomers in order to be able to insert into biological membranes and generate channels. electron microscopy and image analysis of two-dimensional membrane crystals had previously revealed a structure with 7-fold symmetry suggesting that aerolysin forms heptameric oligomers [wilmsen et al. (1992) embo j. 11, 2457-2463]. however, this unusual molecularity of ...19968617369
aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, ompa.two homologs of the outer membrane protein ompa were identified in aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. an a. salmonicida genomic dna library was constructed by using lambda gem-11 and recombinant phage carrying both genes ompai and ompaii) selected by immunoscreening. a 5.0-kb bamhi fragment containing the two genes in tandem was subcloned in pbluescript and used for further subcloning and seque ...19968626290
fatal aeromonas hydrophila bacteremia in a hemodialysis patient treated with deferoxamine.a 49-year-old woman undergoing long-term hemodialysis and treated with deferoxamine (dfo) 1.5 g twice weekly for aluminum bone disease developed fever and bilateral calf pain caused by myonecrosis with gas gangrene. she had a rapidly fatal outcome. the cultures of blood and aspirates from both calf muscles demonstrated aeromonas hydrophila. no obvious entry point could be traced. the in vitro growth of the patient's strain was found to be stimulated by the deferoxamine-iron complex in an iron-de ...19968629637
biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental aeromonas isolates.one hundred and eight strains of aeromonas from clinical and environmental samples were speciated. seven species were identified, the most prevalent of which was a. hydrophila. experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant fluid accumulation in rabbit ileal loops. of 107 strains showing single or multiple antimicrobial resistance, the highest incidence of resistance was shown for beta-lactam antibioti ...19968636960
behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacilli.in this study, behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacillus sp. were determined. ph values of the skim milk samples were also examined during fermentation. the amount of produced lactic acid and diacetyl/acetoin productions of the lactobacillus sp. were estimated. antimicrobial effects of the lactobacilli on y. enterocolitica and a. hydrophila were also determined by an agar diffusion method. while y. enterocolitica was n ...19968638435
enzyme kinetics and biochemical analysis of imis, the metallo-beta-lactamase from aeromonas sobria 163a.the metallo-beta-lactamase from aeromonas sobria 163a, imis, was isolated in a two stage purification procedure using protein affinity columns. enzyme kinetics show that imis hydrolyses the carbapenems but displays poor activity against other beta-lactams. imis possesses the narrowest spectrum of activity of the group 3 enzymes that have been analysed. sequencing of the 40 n-terminal amino acids show this region to be identical to that of the cpha metallo-beta-lactamase from aeromonas hydrophila ...19969182099
production of haemolysin and enterotoxin by aeromonas jandaei and aeromonas trota strains after animal passage.five aeromonas jandaei and 12 aeromonas trota isolates were tested for the production of haemolysin and enterotoxin, and the correlation between these two properties. the majority (10 isolates) of the strains produced beta-haemolysis. the titres of haemolytic activity for both species were 8-64 hu/ml. in the initial ileal loop test, only two (a. trota) of the 17 isolates produced enterotoxin. one each of these 2 a. trota strains was beta-haemolytic and non-haemolytic. the remaining isolates of a ...19969203791
antidiarrhoeal activities of ocimum gratissimum (lamiaceae).the antidiarrhoeal activities of leaf extracts of ocimum gratissimum were investigated by disc diffusion and tube dilution methods. the extracts were active against aeromonas sobria, escherichia coli, plesiomonas shigelloides, salmonella typhi, and shigella dysenteriae. the leaf extracts were most active against s. dysenteriae and least active against s. typhi. the sensitivity of the organisms measured in terms of zone of inhibition ranged from 8.00 to 19.50 mm. the minimum inhibitory concentrat ...19969203793
[purification and characterization of s-layer protein from aeromonas hydrophila].the regular surface protein array (s-layer) present on aeromonas hydrophila j-1 was composed of a single species of protein of apparent molecular weight 51500. this protein was extracted from whole cells by treatment with 0.2 mol/l glycine hydrocholoide (ph4.0). the protein was purified by sephadex g-200 gel filtration chromatography and an ion exchange chromatography on deae-cellulose. amino acid composition analysis showed that the protein contained 36.8% hydrophobic amino acids. but the prote ...19969206273
metabolism of proteins and glycoproteins in tumour bearing mice treated with aeromonas l-asparaginase.l-asparaginase, isolated in our laboratory, from aeromonas had been found to be antileukaemic. in the present study, changes in the levels of proteins and glycoproteins in leukaemic mice and under treatment with aeromonas l-asparaginase have been compared. levels of protein bound hexose, fucose and sialic acid which were increased during leukaemia attained normal levels when treated with l-asparaginase. the increased blood urea level declined significantly during enzyme therapy. effects of l-asp ...19969219441
bacterial diarrhea in laos, a region where cholera was endemic.there were cholera outbreaks in lao pdr since 1993. two provinces were epidemic areas of cholera in 1993. however the area of cholera occurrence increased to 7 provinces through 1994. then a bacterial survey had done for the purpose of public health improvement. epec was markedly isolated from the hospital in vientiane. in apparent infection of salmonella and non-01 vibrio cholerae with soldiers stationed in the island in mekong river belong to vientiane were pointed out. the outbreak of diarrhe ...19969253874
high-resolution genotypic analysis of the genus aeromonas by aflp fingerprinting.we investigated the ability of a recently developed genomic fingerprinting technique, named aflp, to differentiate the 14 currently defined dna hybridization groups (hgs) in the genus aeromonas. we also determined the taxonomic positions of the phenospecies aeromonas allosaccharophila, aeromonas encheleia, aeromonas enteropelogenes, and aeromonas ichthiosmia, which have not been assigned to hgs yet. a total of 98 aeromonas type and reference strains were included in this study. for the aflp anal ...19968934910
study of the intergenic exef-exeg region and its application as a simple preliminary test for aeromonas spp.the exef-exeg intergenic regions from different hybridization groups (hg) of aeromonas were studied by pcr amplification using a single pair of primers. six main classes of pcr products were identified according to size: 360 bp, 320 bp, 280 bp, 230-240 bp, 220 bp and 160 bp. direct sequencing of the pcr products indicated that the shorter intergenic regions had probably originated from deletion of dna segments between direct repeats. correlation of certain pcr products with aeromonas caviae (hg4 ...19968935655
polysaccharide lyases from gellan-producing sphingomonas spp.a number of sphingomonas strains capable of synthesizing the bacterial exopolysaccharide gellan and related polymers were shown to possess constitutive gellanase activity. in each case, the degradation of deacylated gellan was due to extracellular, eliminase-type enzymes (lyases) which cleave the sequence ... beta-d-glucosyl 1,4-beta-d-glucuronosyl ... in the tetrasaccharide repeat unit of the substrate polysaccharides. deacetylated rhamsan was an alternative substrate but there was little or no ...19968936312
monoclonal antibodies against haemophilus influenzae lipopolysaccharides: clone mahi 4 binding to a pentasaccharide containing terminal beta-gal residues and clone mahi 10 recognizing terminal phosphorylated saccharide residues.mouse monoclonal antibodies mahi 4 and mahi 10 reactive with haemophilus influenzae lipopolysaccharide (lps), were generated by fusing mouse myeloma cells with spleen cells of mice immunized with h. influenzae strain rm.7004-xp-1. the antibody mahi 4 reacted in whole-cell enzyme immunoassay (eia) and colony-dot-immunoblotting with 20 of 123 h. influenzae strains and to a few other human haemophilus spp. and neisseria spp., but not to any bordetella pertussis, b. parapertussis, aeromonas spp. or ...19968938639
molecular and biochemical characterization of a heat-labile cytotonic enterotoxin from aeromonas hydrophila.we report herein the dna sequence analysis of the heat-labile cytotonic enterotoxin gene (alt) from aeromonas hydrophila and the biological function of the purified hyperproduced toxin (alt). one large open-reading frame (orf), comprised of 1104 bp, was detected at positions 804 to 1907 bp on a 4.0-kb sa/l dna fragment from aeromonas. this orf encodes for a protein having 368 amino acids (aa) with a computed molecular weight of 38 kda. the aa sequence of the first 15 nh2-terminal residues of the ...19968938643
secretonac: a novel nucleic acid secreted in abundance by pseudomonas aeruginosa. 19968939441
pcr detection of metallo-beta-lactamase gene (blaimp) in gram-negative rods resistant to broad-spectrum beta-lactams.we applied pcr to the rapid detection of the metallo-beta-lactamase gene, blaimp, in clinically isolated gram-negative rods. a total of 54 high-level ceftazidime-resistant strains (mics, > 128 micrograms/ml) were subjected to pcr analyses with the blaimp-specific primers, since the blaimp-bearing clinical isolates tested in our previous study always demonstrated high-level resistance to ceftazidime. twenty-two blaimp-positive strains including 9 pseudomonas aeruginosa, 9 serratia marcescens, 2 a ...19968940421
characterization of cytotoxic, hemolytic aeromonas caviae clinical isolates and their identification by determining presence of a unique hemolysin gene.aeromonas caviae has recently been recognized as an important enteropathogen and its hemolysin is purported to be one of the virulence factors, in this study, a total of 80 clinical isolates of aeromonas spp. were investigated by pcr with synthetic oligonucleotides targeting a cloned hemolysin-encoding sequence from an a. caviae isolate of clinical origin. of the 35 clinical a. caviae isolates tested, only 6 contained the target sequence.19968940472
mesophilic aeromonas sp. serogroup o:11 resistance to complement-mediated killing.the complement activation by and resistance to complement-mediated killing of aeromonas sp. strains from serogroup o:11 were investigated by using different wild-type strains (with an s-layer characteristic of this serogroup) and their isogenic mutants characterized for their surface components (s-layer and lipopolysaccharide [lps]). all of the aeromonas sp. serogroup o:11 wild-type strains are unable to activate complement, which suggested that the s-layer completely covered the lps molecules. ...19968945581
acid sensitivity as affected by physico-chemical stresses in aeromonas hydrophila.this work focuses mainly on the effect of nacl along with other mono and divalent anions and cations at ph 4 on survival and virulence properties of aeromonas hydrophila. to find the optimum stress condition, effects of several other physical factors on nacl induction were also assayed. a. hydrophila collected from international center for diarrheal disease research, bangladesh (icddr,b) was found to be more sensitive to ph 4.0 when grown in media containing 200 mm or more salt. induction of aci ...19968950641
isolation and characterization of restriction endonuclease in plesiomonas shigelloides and aeromonas species.five restriction endonucleases (enases) and one enase were found in a screen of 196 strains of plesiomonas shigelloides and 147 strains of aeromonas species. plesiomonas and aeromonas species are classified as vibrionaceae, identified as food-poisoning bacteria, are closely genetically related to each other, and their enases producing abilities have not bee reported. enases were detected at relatively low frequencies in these species as compared to those in other species, such as salmonella spec ...19968951174
a tonb-like protein and a novel membrane protein containing an atp-binding cassette function together in exotoxin secretion.protein secretion by many gram-negative bacteria occurs via the type ii pathway involving translocation across the cytoplasmic and outer membranes in separate steps. the mechanism by which metabolic energy is supplied to the translocation across the outer membrane is unknown. here we show that two aeromonas hydrophila inner membrane proteins, exea and exeb, are required for this process. exeb bears sequence as well as topological similarity to tonb, a protein which opens gated ports for the inwa ...19968951808
[a case of severe acute hepatorenal failure due to aeromoas hydrophila septicemia].a case of septicemia due to aeromonas hydrophila (a. hydrophila) in a 54-year-old male suffering from progressive severe jaundice and fatigue is reported. the patient developed multiple organ failure despite aggressive therapy including plasma exchange and glucose-insulin therapy. upon admission to our hospital, therapy was started with ampicillin (abpc) 4 g/day, gentamicin (gm) 120 mg/day, hemodialysis, continuous hemofiltration, catecholamines and a respirator, but he expired on the 2nd hospit ...19968952274
expression of chitinase-encoding genes from aeromonas hydrophila and pseudomonas maltophilia in bacillus thuringiensis subsp. israelensis.fifty isolates of chitinase (cts)-producing bacteria were collected from soil samples and tested for their ability to degrade chitin using colloidal chitin agar as the primary plating medium. the results indicated that three isolates could degrade chitin at high ph. further studies also demonstrated that crude cts preparations from bacillus circulans (bc) no. 4.1 could enhance the toxicity of bacillus thuringiensis subsp. kurstaki (bt-k) toward diamondback moth larvae. thus, it might be useful t ...19968955637
water quality parameters associated with aeromonas spp-affected hatcheries.this study investigates the role of water quality parameters as possible risk or protection factors in aeromonas spp-affected rainbow trout hatcheries in northeastern spain. the results revealed an association between oxygen concentration, ammonia concentration and total dissolved solids (tds) with the prevalence of aeromonas spp cases on the affected fish farms. the oxygen and ammonia concentrations acted as risk factors when their values were lower than 7.06 mg/l or higher than 0.05 mg/l, resp ...19968956470
furunculosis control using a paraimmunization stimulant (baypamun) in rainbow trout.the effect of baypamun, a non-specific immune system stimulant, on the treatment and control of furunculosis was studied in experimentally infected rainbow trout. a statistically significant association between the baypamun treatment and a reduction in cumulative incidence of the clinical symptoms of furunculosis and the infection level, despite the presence of the bacteria in blood and organs was observed. a reduction in the mortality rate in baypamun-treated fish was also observed but this was ...19968956471
adherence of aeromonas caviae to human cell lines hep-2 and caco-2.adherence of aeromonas caviae to hep-2 and caco-2 cell monolayers was investigated with 24 clinical isolates. growth phase, temperature, multiplicity of infection and length of incubation affected adherence. treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin b and cycloheximide to the monolayer significantly reduced the adherence capabilities of the strains investigated. the use of chloramphenicol to inhibit protein synthesis reduce ...19968958248
indigenous bacterial flora of medicinal leeches and their susceptibilities to 15 antimicrobial agents.surface bacterial flora, as well as homogenates, of medical leeches, hirudo medicinalis and hirudinaria manillensis, were surveyed and the susceptibility of these isolates to 15 antimicrobial agents was examined. aeromonas spp. were isolated from all leeches, and pseudomonas fluorescens and other glucose-non-fermenting gram-negative rods (nf-gnr) were frequent isolates. isolates were highly resistant to cephalosporins but susceptible to carbapenems, aminoglycosides and ofloxacin. the results ind ...19968958255
pathological and microbiological findings from incidents of unusual mortality of the common frog (rana temporaria).in 1992 we began an investigation into incidents of unusual and mass mortalities of the common frog (rana temporaria) in britain which were being reported unsolicited to us in increasing numbers by members of the public. investigations conducted at ten sites of unusual mortality resulted in two main disease syndromes being found: one characterized by skin ulceration and one characterized by systemic haemorrhages. however, frogs also were found with lesions common to both of these syndromes and m ...19968962441
near-drowning-associated aeromonas pneumonia.aeromonas is increasingly recognized as a human pathogen that causes a variety of different infections. aeromonas has rarely been reported as a cause of respiratory infection, and it has been described in near-drowning-associated pneumonia. this article reviews a case of aeromonas sobria pneumonia associated with a near drowning and considers the clinical and epidemiological characteristics of 10 previously reported cases. nearly all of the cases involved young healthy men, a rapid development o ...19968969997
the cell surface of aeromonas salmonicida determines in vitro survival in cultured brook trout (salvelinus fontinalis) peritoneal macrophages.aeromonas salmonicida strains phenotypically differing in their a-layer, lipopolysaccharide, and macrophage cytotoxicity were tested in vitro for survivability in brook trout (salvelinus fontinalis) serum with or without antibodies, and in vivo following intraperitoneal injection. the ability of brook trout peritoneal macrophages to phagocytize and kill the different phenotypes was investigated in an in vitro assay. the virulent strain, a. salmonicida 80204, which has the full complement of know ...19968971685
immunological analysis of extracellular products and cell surface components of motile aeromonas isolated from fish.the present work describes the characterization of antigens present in the extracellular products (ecp) and cell wall of strains of motile aeromonas isolated from rainbow trout culture systems. the relationships among virulence for fish, o-serogroup and profile of lps were also examined. the slide agglutination test showed that most of the virulent strains of motile aeromonas (72%) were included in the serotypes o3, o6, o11 and o19 (guinée and jansen system). however, there were also non-pathoge ...19968972085
survival potential of aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria.the survival of a genetically-marked aeromonas hydrophila strain was studied in water microcosms using viable counts. aeromonas hydrophila awwx1 was shown to survive without decline in viable counts for at least 10 d in three of four filtered-autoclaved freshwaters (surface water and groundwater) and in all examined filtered-autoclaved nutrient-poor waters (bottled spring water, milli-q and tap water). however, in the unfiltered waters, a rapid decrease in viable counts of aer. hydrophila awwx1 ...19968852674
pulsed-field gel electrophoresis as an epidemiological tool for clonal identification of aeromonas hydrophila.pulsed-field gel electrophoresis (pfge) was used to characterize aeromonas hydrophila strains isolated from a cluster of hospital-acquired infections that occurred over approximately 1 month in a french hospital. five isolates from patients and 10 isolates from the water supply were characterized by biotyping and antibiotic susceptibility patterns and compared with 10 epidemiologically unrelated strains isolated from patients and rivers, by pfge of digests of chromosomal dna. five environmental ...19968852675
hlya hemolysin of vibrio cholerae o1 biotype e1 tor. identification of the hemolytic complex and evidence for the formation of anion-selective ion-permeable channels.hemolysin (hlya) was concentrated from supernatants of different vibrio cholerae o1 biotype e1 tor strains by ammonium sulfate precipitation. the concentration of the toxin in the supernatants and in the precipitates was quantified using its hemolytic activity. the toxin formed a high molecular-mass band (about 220 kda) on sds/page while the toxin monomer had a molecular mass of 60 kda when it was heated. the addition of the e1 tor hemolysin oligomers, but not that of the monomers, to the aqueou ...19968856066
hazard identification in swine slaughter with respect to foodborne bacteria.swine slaughter is an open process with many opportunities for the contamination of the pork carcass with potentially pathogenic bacteria; however, it does not contain any point where hazards are completely eliminated. data on the prevalence of various pathogenic bacteria (aeromonas hydrophila, campylobacter coli/jejuni, listeria monocytogenes, salmonella spp., staphylococcus aureus and yersinia enterocolitica) in pigs, their growth and survival characteristics and ability to become established ...19968856371
diarrheal and environmental isolates of aeromonas spp. produce a toxin similar to shiga-like toxin 1.diarrheal and environmental isolates of 39 strains of aeromonas spp. were studied for detection of virulence factors. although these 39 strains did not produce either heat-labile or heat-stable enterotoxins, culture filtrates of 31 strains produced cytopathic effects on vero cells. among these, culture filtrates of three strains of aeromonas hydrophila and one strain of aeromonas caviae could be neutralized by escherichia coli o157:h7 shiga-like toxin 1 antiserum. a single band of plasmid dna of ...19968857271
structural studies of the o-antigenic polysaccharide from an aeromonas caviae strain.the structure of the o-antigenic polysaccharide from a strain of aeromonas caviae, isolated from the stools of a patient with diarrhoea, has been investigated. sugar analysis, methylation analyses, and a uronic acid degradation together with nmr spectroscopy were the principal methods used. the sequence of the sugar residues could be determined by noesy and hmbc experiments. it is concluded that the polysaccharide is composed of pentasaccharide repeating units with the following structure: [sequ ...19968864229
comparative analysis of the cya locus in enterobacteria and related gram-negative facultative anaerobes.comparison of the cya loci (cya codes for adenylyl cyclase (ac)) from a variety of phylogenetically divergent facultative anaerobic gram-negative bacteria reveals conserved sequence features. the entire locus structure in enterobacteria is preserved, including two major promoters (a conserved cya strong promoter, p2, and a divergent promoter for a heme biosynthetic operon, hemcd) present in the upstream region of the cya gene. the region between hemc and cya is much longer in proteus mirabilis t ...19968874804
cloning and detection of the hemolysin gene of vibrio anguillarum.a 5 kb dna fragment encoding a hemolysin was cloned from the fish pathogenic bacterium vibrio anguillarum using the cosmid vector charomid9-36. an open reading frame of the hemolysin gene (vah1) was 2253 bp and corresponded to a protein of 751 amino acid residues. the deduced amino acid sequence of the vah1 gene and the previously reported vibrio cholerae ei tor hemolysin, v. vulnificus cytolysin-hemolysin, aeromonas hydrophila ahh1 hemolysin, and a. salmonicida ash4 hemolysin showed a significa ...19968878014
thermal and biological treatments to control psychrotrophic pathogens.over the past decade, advances in egg processing technologies have permitted commercial production of ultrapasteurized liquid whole egg (lwe) products with a shelf-life of greater than 10 wk at 4 c. the inactivation and control of psychrotrophic pathogens such as listeria monocytogenes and aeromonas hydrophila in extended shelf-life lwe and conventionally pasteurized egg products is an ongoing food safety concern. this manuscript reports on the common features of these two psychrotrophic pathoge ...19968878273
use of the fluorescent dye picogreen for quantification of pcr products after agarose gel electrophoresis. 19968879566
changes in microbial populations on fresh cut spinach.the microbial populations found on fresh-cut spinach leaves that were stored in gas permeable bags at 10 degrees c for 12 days were examined and identified. the microorganisms consisted of mesophilic aerobic bacteria, psychrotrophic bacteria, pseudomonadaceae, enterobacteriaceae, micrococcaceae, lactic acid bacteria and yeasts. populations of mesophiles, psychrotrophs, pseudomonadaceae and enterobacteriaceae increased sharply during the storage period. the initial populations were 10(7), 10(6), ...19968880301
incidence and toxigenicity of aeromonas hydrophila in seafood.three selective media, oxoid aeromonas agar (oa), blood ampicillin agar (ba) and starch ampicillin agar (sa) were used to evaluate the presence of aeromonas hydrophila in 66 samples of oyster, shrimp, fish and surimi products. oyster had the highest incidence, with 50% positive, whilst no a. hydrophila was found in the surimi. of the three selective media, ba displayed the highest recovery rate of a. hydrophila from seafood. forty-eight isolates from this survey were tested for their capability ...19968880302
sparfloxacin worldwide in vitro literature: isolate data available through 1994.sparfloxacin is a piperazinyl, cyclopropyl-fluoroquinolone with broad-spectrum antibacterial activity. compared to other quinolones, sparfloxacin displays improved activity against a variety of pathogens including staphylococcus, streptococcus, enterococcus, chlamydia, mycoplasma, ureaplasma, and mycobacteria species. other susceptible organism group include haemophilus, legionella, moraxella, neisseria, aeromonas, acinetobacter, bordetella, brucella, campylobacter, gardnerella, and helicobacter ...19968882890
the cardiopulmonary, eicosanoid, and tissue microanatomic effects of fluconazole during graded bacteremia.imidazole compounds have been shown to be beneficial in systemic sepsis and inflammation. the purpose of this study was to delineate the effects of fluconazole on systemic hemodynamics and on microanatomy of the heart, lung, liver, and kidney parenchyma of swine during graded bacteremia. eighteen adult swine were studied in three groups: 1), anesthesia control; 2), septic control (aeromonas hydrophila, 10(9)/ml, infused i.v. for 4 h); 3) fluconazole (fluconazole, 30 mg/kg i.v., followed by a. hy ...19968885087
expression of aeromonas caviae bla genes in escherichia coli.an isolate of aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. transfer of the plasmid to escherichia coli k12 resulted in the expression of a tem-like beta-lactamase that was not expressed in parental a. caviae. the bla gene sequence was detectable by dna hybridization and pcr amplification of the plasmid when extracted from parental a. caviae or from e. coli k12 transconjugants. sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing fr ...19968889718
holy water--a risk factor for hospital-acquired infection.a case of hospital-acquired infection due to acinetobacter baumanii in a burns patient after exposure to holy water is described. in order to assess the infection risk, 13 samples of holy water were cultured for bacteria, (including legionellae) and yeasts. viable bacterial counts ranged from 1 center dot 3 x 10(3)-3 center dot 8 x 10(8) cfu/l (mean 3 center dot 1 x 10(7) cfu/l). a wide range of bacterial species was isolated including pseudomonas aeruginosa, enterobacter spp. escherichia coli a ...19968904372
a biochemical protocol for the differentiation of current genomospecies of aeromonas.a data file consisting of 40 biochemical and physiological tests for the differentiation of aeromonads was constructed based upon existing published data from various geographical locations and laboratories. the data file covers all the current genomospecies (or hybridisation groups) of aeromonas and is therefore applicable to bacteriologists from a wide variety of fields. the protocol derived from the data file was adapted for use with a semi-interactive computer identification program. this pr ...19968837366
[gastroenteritis due to aeromonas in pediatrics].in recent years the incidence of gastroenteritis due to aeromonas spp has increased significantly. the purpose of this study was to investigate the etiology and clinical manifestations of this disease.19968849096
differentiation of aeromonas genomospecies using random amplified polymorphic dna polymerase chain reaction (rapd-pcr).random amplified polymorphic dna polymerase chain reaction (rapd-pcr) was used to investigate the differentiation of the genus aeromonas at the genomospecies level. of 20 primers evaluated, six produced profiles which contained multiple bands capable of differentiating the genomospecies. these six primers were also used in rapd-pcr analyses of clinical and environmental isolates of the different genomospecies. in most cases, each strain gave a unique fingerprint, illustrating genetic heterogenei ...19968849642
using a new inbred fish model and cultured fish tissue cells to study aeromonas hydrophila and yersinia ruckeri pathogenesis.an inbred strain of the southern platyfish, xiphophorus maculatus, was used as a host for aeromonas hydrophila and yersinia ruckeri infections. the infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent a. hydrophila or y. ruckeri strains. inoculating the platyfish in this manner resulted in a dose-dependent mortality over a range of bacterial input from 10(5) to 10(8) a. hydrophila and 10(6) to 10(8) y. ruckeri/ml. clinical manifestations of a. ...19968722100
prevalence and serovar distribution of salmonella in fresh and frozen meat from captive nile crocodiles (crocodylus niloticus).salmonella was isolated from 6 of 20 (30.0%) samples of fresh meat, and from 28 of 140 (20.0%) samples of frozen meat processed for human consumption from captive nile crocodiles (crocodylus niloticus) in zimbabwe. salmonella enterica isolates showed a serovar distribution of 41.2% (14/34) subsp. enterica, 11.8% (4/34) subsp. salamae and 41.2% (14/34) subsp. diarizonae. analyses of fresh meat samples yielded aeromonas (a. hydrophila) in 18 of 20 samples (90%), and a mean aerobic plate count (apc ...19968722192
development of aeromonas hydrophila bacteremia in a patient recovering from cholera. 19968722957
cloning and nucleotide sequence of the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida.the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida 2148/89 was cloned, and the nucleotide sequence was determined. an open reading frame of 2,766 nucleotides was identified and was found to encode a protein of 922 amino acids with a calculated molecular mass of 101.1 kda. the derived amino acid sequence shared a high degree of identity with other dna gyrase a proteins, in particular, with other gram-negative gyra sequences. when the amino acid sequence of a. salmonicida gyra w ...19968723452
increasing antibiotic resistance in clinical isolates of aeromonas strains in taiwan.a total of 234 clinical isolates of aeromonas, primarily a. hydrophila, were collected for the present study. most were isolates from blood. by the agar dilution method, more than 90% of the aeromonas strains were found to be susceptible to moxalactam, ceftazidime, cefepime, aztreonam, imipenem, amikacin, and fluoroquinolones, but they were more resistant to tetracycline, trimethoprim-sulfamethoxazole, some extended-spectrum cephalosporins, and aminoglycosides than strains from the united states ...19968723478
exploring the use of the medicinal leech: a clinical risk-benefit analysis.the medicinal leech, hirudo medicinalis, has been used with increasing frequency for salvage of compromised pedicled flaps and microvascular free-tissue transfers. the data pertaining to 18 cases in which flap salvage with leeches was attempted were reviewed and contrasted with the data from 108 published cases from other centers. a further analysis isolated and examined 19 cases in which infection, secondary to leech utilization, supervened. results suggested that successful salvage of tissue w ...19968726336
a live (delta aroa) aeromonas salmonicida vaccine for furunculosis preferentially stimulates t-cell responses relative to b-cell responses in rainbow trout (oncorhynchus mykiss).we have previously described (l. m. vaughan, p. r. smith, and t. j. foster, infect. immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant of aeromonas salmonicida, the causative agent of furunculosis, and its use as a live vaccine. here we describe the construction of an unmarked aroa deletion mutant and examine the nature and magnitude of immune responses in rainbow trout (oncorhynchus mykiss) to this vaccine strain. good proliferative and antibody respo ...19968751940
evaluation of the dna fingerprinting method aflp as an new tool in bacterial taxonomy.we investigated the usefulness of a novel dna fingerprinting technique, aflp, which is based on the selective amplification of genomic restriction fragments by pcr, to differentiate bacterial strains at the subgeneric level. in totals, 147 bacterial strains were subjected to aflp fingerprinting: 36 xanthomonas strains, including 23 pathovars of xanthomonas axonopodis and six pathovars of xanthomonas vasicola, one strain of stenotrophomonas, 90 genotypically characterized strains comprising all 1 ...19968757752
application of antimicrobial-producing lactic acid bacteria to control pathogens in ready-to-use vegetables.five psychrotrophic strains of lactic acid bacteria (lactobacillus casei, lact. plantarum and pediococcus spp.) were isolated from 22 samples of commercial salads. these strains were shown to inhibit aeromonas hydrophila, listeria monocytogenes, salmonella typhimurium and staphylococcus aureus on mrs agar, in salads and in juice prepared from vegetable salads. lactobacillus casei impclc34 was most effective in reducing total mesophilic bacteria and the coliform group; aer. hydrophila, salm. typh ...19968760320
antimicrobial susceptibility and plasmid-mediated streptomycin resistance of plesiomonas shigelloides isolated from blue crab.five plesiomonas shigelloides strains isolated from retail or wild-catch louisiana blue crabs (callinectes sapidus) were examined for resistance to selected antibiotics and presence of plasmids. three agar media, macconkey, salmonella-shigella and a modification of inositol brilliant green bile salt (ibb), were evaluated for isolation of p. shigelloides. of these, ibb was most satisfactory for distinguishing between p. shigelloides and the related species aeromonas hydrophila. isolates were susc ...19968760329
platelet activating factor mediates cardiopulmonary dysfunction during graded bacteremic shock.to determine whether or not platelet activating factor (paf) is a necessary mediator of cardiovascular dysfunction during graded bacteremia, and to identify paf interactions with eicosanoids and tumor necrosis factor-alpha (tnf-alpha).19968760539
[rapid detection of salmonella with mucap reagent].the 4-methylumbelliferyl-caprylate (mucap) was applied for the identification of salmonella, it's specification, sensitity, and availability were reported. 65 strains of standard salmonella, 48 strains of salmonella isolated from foods growing on plates of he, dhl, ss and maconkey agar have been tested with mucap. all of them were identified as mucap positive; whereas, among the non-salmonella tested only pseudamonas spp., aeromonas hydrophilia and plesiomonas shigelloides were shown as positive ...19968762473
species and serovars of enteropathogenic agents associated with acute diarrheal disease in rosario, argentina.we report the most frequent species and serovars of enteropathogenic organisms in rosario from 1985 to 1993. enteropathogenic escherichia coli was the most prevalent agent affecting 144/570 (25.2%) children; 0111 represented 41.8%, 055: 13.6%, 0119: 12.7%. among enterotoxigenic e. coli (etec) the most frequent were etec-st 0128:h21 and 0153:h45. shigella spp were isolated in 8.8%; s. flexneri: 7%, principally type 2 (59.5%); s. sonnei: 1.6%, and s. dysenteriae type 2: 0.2%. campylobacter spp wer ...19968762632
atypical aeromonas salmonicida isolated from diseased turbot (scophtalmus maximus l.).atypical aeromonas salmonicida were isolated from 3 outbreaks of disease among farmed turbot (scophthalmus maximus l.) in 3 different farms, 1 from norway (n1) and 2 from denmark (dk1 and dk2). in all 3 cases, the incidence of disease and mortality was high and the main characteristic pathological finding was skin ulcers and septicaemia. the isolated bacteria were subjected to a thorough phenotypic and genotypic examination and comparison in the laboratory. all 3 isolates belonged to a. salmonic ...19968767692
aetiological role of aeromonas and plesiomonas species in human diarrhoeal illness in nigeria. 19968771938
a fresh water pathogen and its pedal manifestations. 19968775178
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