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carp interleukin-1 beta in the role of an immuno-adjuvant. 200010938746
outer membrane proteins of aeromonas hydrophila induce protective immunity in goldfish. 200010938747
production of siderophores & effect of iron restriction on the protein profiles of aeromonas species isolated from water & patients suffering from acute diarrhoeal disease.among 48 strains of aeromonas species, 21 isolates from patients suffering from acute diarrhoea and 27 from metropolitan water samples grown under iron-restricted conditions, 45 strains produced siderophores. forty one of the 46 strains tested produced siderophores on chrome azurol s (cas) agar, while 43 isolates did so when the culture supernatants of the bacterial isolates grown in minimal medium were assayed with chrome azurol s assay solution. the whole cell protein profiles of a. hydrophila ...200010943068
prevalence and virulence properties of vibrio cholerae non-o1, aeromonas spp. and plesiomonas shigelloides isolated from cambé stream (state of paraná, brazil).the incidence of vibrio cholerae, aeromonas spp. and plesiomonas shigelloides was determined in water samples from cambé stream. the samples were collected from seven different sites. the serogroups, virulence markers and drug resistance profiles were also evaluated. twelve aer. hydrophila, 12aer. caviae, eight aer. sobria, seven ple. shigelloides and two v. cholerae non-o1 were isolated. they belonged to different serogroups and all produced haemolysis in different assays. five of the aeromonas ...200010945781
denitrification by indigenous microbial populations of river water measured using membrane inlet mass spectrometry.the major process that reduces nitrate levels in soils and water is denitrification, which converts nitrate and nitrite into gaseous forms of nitrogen, which are then released into the atmosphere. this study used membrane inlet mass spectrometry (mims) to investigate denitrification in river water bacterial isolates supplied with nitrate and succinate as an energy source as well as in the total population by provision of different carbon compounds to untreated river water samples. substantial va ...200010945788
the response of aeromonas hydrophila to oxidative stress induced by exposure to hydrogen peroxide.aeromonas hydrophila, an opportunist human pathogen of low virulence, was shown to display a high degree of sensitivity upon exposure to hydrogen peroxide. as with other species, aer. hydrophila is able to develop the capacity to resist loss of viability induced by such oxidative stress. development of stress resistance follows the archetypal profile where pre-exposure of a population to sub-lethal levels of h2o2 stimulates onset of tolerance to further exposure. acquisition of tolerance critica ...200010945791
occurrence and seasonal variation of airborne gram negative bacteria in a sewage treatment plant.a study was carried out to determine the microbial density and the seasonal variation of airborne gram negative bacteria in a sewage treatment plant. sampling was made at 16 sites and the settle plate technique was used. of the 201 samples examined, 43.2% revealed fecal coliforms (mean value = 14 cfu/p/h), 53% pseudomonas spp. (mean value = 11 cfu/p/h), 46.5% shigella spp. (mean value = 13 cfu/p/h), 3% legionella spp. (mean value = 2 cfu/p/h) and 2% salmonella (mean value = < 1 cfu/p/h). 72% of ...200010946412
grouping by plasmid profiles of atypical aeromonas salmonicida isolated from fish, with special reference to salmonid fish.plasmid profile analyses were performed for 113 strains of atypical aeromonas salmonicida and the reference strain a. salmonicida subsp. salmonicida atcc 14174. the atypical a. salmonicida strains comprised 98 strains obtained from fish originating from 54 farms and 2 lakes in norway, 10 strains from canada (2), denmark (2), finland (1), iceland (1) and sweden (4), the reference strains ncmb 1109 and atcc 15711 (haemophilus piscium) of a. salmonicida subsp. achromogenes, and the type cultures a. ...200010950178
intestinal cell adhesion and maximum growth temperature of psychrotrophic aeromonads from surface water.investigation of 650 aeromonas strains taken from surface water--used as a source of drinking water--and from the associated drinking water treatment plants has demonstrated that only a small proportion of the strains are endowed with intestinal cell adhesion and growth at 39 degrees c; these characteristics are concurrently encountered in aeromonas strains from clinical material. the investigations permit the conclusion that even the aeromonas species, of which certain strains are pathogenic to ...200010956594
inhibition of aeromonas caviae and a. sobria by sodium choloride, citric acid, ascorbic acid, potassium sorbate and extracts of thymus vulgaris.the respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and thymus vulgaris extract on the growth of aeromonas caviae and aeromonas sobria were investigated. sodium chloride (3%) significantly reduced the growth and 4% nacl inhibited growth of the tested strains. ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inhibited growth. t. vulgaris extract (0.3%) greatly reduced the growth. various combinations of these com ...200010957708
distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of tn1721 in dissemination of the tetracycline resistance determinant tet a.oxytetracycline-resistant (ot(r)) mesophilic aeromonads were recovered from untreated hospital effluent (72 isolates) and from fish farm hatchery tanks (91 isolates) at sites within the english lake district, cumbria, england. the transfer of ot(r) plasmids from these isolates was investigated. using escherichia coli j53-1 as a recipient, 11 isolates from the hospital site and 6 isolates from the fish farm site transferred ot(r) plasmids (designated pfbaot1 to 17). original isolates were identif ...200010966404
lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.).the present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.). a relatively low population level (approximately 2 x 103 bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobac ...200010971765
spontaneous bacterial empyema caused by aeromonas veronii biotype sobria.spontaneous bacterial empyema is a complication of hepatic hydrothorax in cirrhotic patients. the pathogen, clinical course and treatment strategy are different to the empyema secondary to pneumonia. a 54-year-old man, who was a cirrhotic patient with hepatic hydrothorax, was admitted to national taiwan university hospital for fever, dyspnea and right side pleuritic pain. the image study revealed massive right pleural effusion and no evidence of pneumonia. the culture of pleural effusion yielded ...200010974579
[cultivation optimization of prolyl endopeptidase and high cell density fermentation].engineered e. coli bl21/pgem-pep could constitutively express recombinant prolyl endopeptidase from aeromonas punctata, which was extremely effected by culture conditions, so fermentation conditions were optimized to obtain it's high expression level. firstly, the stability of bl21/pgem-pep was investigated, then, culture temperature, ph, time, medium were optimized in shake flaskd. the l9(3(4)) orthogonal experiment confirmed that shaker speed, ph, culture temperature, culture time had high deg ...200010976323
convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds.a simple disk diffusion test was constructed for detection of imp-1-type metallo-beta-lactamase-producing gram-negative bacteria. two kirby-bauer disks containing ceftazidime (caz) and a filter disk containing a metallo-beta-lactamase inhibitor were used in this test. several imp-1 inhibitors such as thiol compounds including 2-mercaptopropionic acid, heavy metal salts, and edta were evaluated for this test. two caz disks were placed on a mueller-hinton agar plate on which a bacterial suspension ...200010618060
impact of an urban effluent on antibiotic resistance of riverine enterobacteriaceae and aeromonas spp.in order to evaluate the impact of an urban effluent on antibiotic resistance of freshwater bacterial populations, water samples were collected from the arga river (spain), upstream and downstream from the wastewater discharge of the city of pamplona. strains of enterobacteriaceae (representative of the human and animal commensal flora) (110 isolates) and aeromonas (typically waterborne bacteria) (118 isolates) were selected for antibiotic susceptibility testing. most of the aeromonas strains (7 ...200010618213
carboxy terminal region of haemolysin of aeromonas sobria triggers dimerization.haemolysin of aeromonas sobria is released into the culture supernatant in the form of prohaemolysin. removal of a 42 amino acid peptide at the carboxy-terminal end converts prohaemolysin into mature haemolysin. as the role of the peptide removed from the mature haemolysin has not been studied, we mutated the haemolysin genes to delete several amino acid residues from the carboxy terminus, expressed the mutant genes in a. sobria and analysed the haemolysins produced. deletion of more than three ...200010623561
two regions of epsl involved in species-specific protein-protein interactions with epse and epsm of the general secretion pathway in vibrio cholerae.extracellular secretion of proteins via the type ii or general secretion pathway in gram-negative bacteria requires the assistance of at least 12 gene products that are thought to form a complex apparatus through which secreted proteins are translocated. although this apparatus is specifically required only for the outer membrane translocation step during transport across the bacterial cell envelope, it is believed to span both membranes. the epse, epsl, and epsm proteins of the type ii apparatu ...200010633109
glycine metabolism in candida albicans: characterization of the serine hydroxymethyltransferase (shm1, shm2) and threonine aldolase (gly1) genes.genes encoding the mitochondrial (shm1) and cytosolic (shm2) serine hydroxymethyltransferases, and the l-threonine aldolase gene (gly1) from candida albicans were cloned and sequenced. all three genes are involved in glycine metabolism. the c. albicans shm1 protein is 82% identical to that from saccharomyces cerevisiae and 56% identical to that from homo sapiens. the corresponding identities for the shm2 proteins are 68% and 53%. the gly1 protein shares significant identity with the s. cerevisia ...200010641038
isolation of bacteria other than helicobacter pylori from stomachs of squirrel monkeys (saimiri spp.) with gastritis.gastric biopsy specimens obtained from 12 squirrel monkeys (saimiri spp.) were investigated by culture for the presence of bacteria. the stomachs of two monkeys with gastritis were colonized with gram-negative, urease-positive bacteria, identified as ochrobactrum anthropi by the vitek and api nft methods (biomérieux). a third monkey with gastritis was positive for aeromonas salmonicida and pseudomonas vesicularis (both urease-negative). no helicobacter pylori was isolated from squirrel monkeys. ...200010711437
molecular cloning of two (r)-specific enoyl-coa hydratase genes from pseudomonas aeruginosa and their use for polyhydroxyalkanoate synthesis.two pseudomonas aeruginosa genes, termed phaj1(pa) and phaj2(pa), homologous to the aeromonas caviae (r)-specific enoyl-coa hydratase gene (phaj(ac)) were cloned using a pcr technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (pha) synthesis from beta-oxidation cycle. two expression plasmids for phaj1(pa) and phaj2(pa) were constructed and introduced into escherichia coli dh5alpha strain. the recombinants harboring phaj1(pa) or phaj2(pa) showed high (r)-specific enoyl ...200010713420
inhibition of the aminopeptidase from aeromonas proteolytica by l-leucinethiol: kinetic and spectroscopic characterization of a slow, tight-binding inhibitor-enzyme complex.the peptide inhibitor l-leucinethiol (leush) was found to be a potent, slow-binding inhibitor of the aminopeptidase from aeromonas proteolytica (aap). the overall potency (k(i)*) of leush was 7 nm while the corresponding alcohol l-leucinol (leuoh) was a simple competitive inhibitor of much lower potency (k(i) = 17 microm). these data suggest that the free thiol is likely involved in the formation of the e x i and e x i* complexes, presumably providing a metal ligand. in order to probe the nature ...200010714704
genetic diversity of the fish pathogen aeromonas salmonicida demonstrated by random amplified polymorphic dna and pulsed-field gel electrophoresis analyses.the current taxonomy of aeromonas salmonicida includes 4 subspecies. a. salmonicida subsp. salmonicida is associated with salmonid furunculosis, and a. salmonicida subsp. achromogenes, a. salmonicida subsp. masoucida, and a. salmonicida subsp. smithia are strains that show variation in some biochemical properties. this classification does not readily encompass isolates from a wide range of fish hosts currently described as atypical a. salmonicida. this study examined 17 typical strains, 39 atypi ...200010715816
innate immunity and the normal microflora.this paper discusses the following ten subtitles with the contents indicated. 1. to meet a microbe: discusses the four alternatives in host-microbe interactions. 2. receptors and signal transduction giving gene activation: discusses the lipopolysaccharide receptor and the limitations of cell cultures versus use of live animals. 3. effector molecules--antimicrobial peptides with and without cysteines. a data base exists with over 500 sequences. this paper gives a general overview of five classes ...200010719663
characterization of vim-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a pseudomonas aeruginosa clinical isolate in france.pseudomonas aeruginosa col-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in marseilles, france, in 1996. this strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. the carbapenem-hydrolyzing beta-lactamase gene of p. aeruginosa col-1 was cloned, sequenced, and expressed in escherichia coli dh10b. the deduced 266-amino-a ...200010722487
cloning, sequencing, and role in serum susceptibility of porin ii from mesophilic aeromonas hydrophila.we cloned and sequenced the structural gene for aeromonas hydrophila porin ii from strain ah-3 (serogroup o:34). the genetic position of this gene, like that of ompf in escherichia coli, is adjacent to aspc and transcribed in the same direction. however, upstream of the porin ii gene no similarities with e. coli were found. we obtained defined insertion mutants in porin ii gene either in a. hydrophila (o:34) or a. veronii sobria (serogroup o:11) serum-resistant or -sensitive strains. furthermore ...200010722573
growth of aeromonas species on increasing concentrations of sodium chloride.the growth of 16 strains of aeromonas, representing 12 species of the genera, were examined at different salt levels (0-1.71 m nacl). all the strains grew on media with 0.34 m nacl, and nine on media with 0.68 m. two strains, aer. enteropelogenes and aer. trota, were able to grow on media with 0.85 m and 1.02 m nacl, respectively. comparison of the growth curves of aer. hydrophila atcc7966 and aer. trota atcc 49657 on four concentrations of nacl (0.08, 0.34, 0.68 and 1.02 m) confirm the high tol ...200010728562
prevalence and diversity of aeromonas and vibrio spp. in coastal waters of southern italy.a survey was undertaken to examine sea water and sediment for the presence of vibrio and aeromonas spp. along approximately 900 km of coast in southern italy during early and late summer. a quantitative analysis was also done to evaluate the water fecal contamination at the stations examined. the results indicate that all the investigated areas were submitted to a wide spatial fluctuation of fecal contamination and that vibrio and aeromonas spp. were present in both high and low fecal-contaminat ...200010660258
production of an enterotoxin by a gastro-enteritis-associated aeromonas strain.the potential of motile aeromonas species to cause human gastrointestinal infections has been recognised recently. considerable worldwide epidemiological, microbiological and clinical investigations have shown that some strains of the different motile aeromonads are of increasing enteropathogenic significance, especially in children, the elderly and in immunocompromised individuals. some of the diarrhoeal symptoms of aeromonas-associated gastro-enteritis have been attributed to enterotoxins. in ...200010670562
kinetic and spectroscopic characterization of native and metal-substituted beta-lactamase from aeromonas hydrophila ae036.two metal ion binding sites are conserved in metallo-beta-lactamase from aeromonas hydrophila. the ligands of a first zinc ion bound with picomolar dissociation constant were identified by exafs spectroscopy as one cys, two his and one additional n/o donor. sulfur-to-metal charge transfer bands are observed for all mono- and di-metal species substituted with cu(ii) or co(ii) due to ligation of the single conserved cysteine residue. binding of a second metal ion results in non-competitive inhibit ...200010675542
quantitative determination of total coliforms and escherichia coli in marine waters with chromogenic and fluorogenic media.this study compared the performance of lmx(r) broth (lmx), chromocult coliform(r) agar (cc) and chromocult coliform agar plus cefsulodin (10 microg ml-1) (cc-cfs), with standard methods multiple tube fermentation (mtf), for the enumeration of total coliforms and escherichia coli from marine recreational waters. lmx and cc are two media designed to concurrently detect total coliform (tc) bacteria and e. coli by the specific action of beta-galactosidase (total coliforms) and beta-glucuronidase (e. ...200010735996
purification and some properties of high-molecular-weight xylanases, the xylanases 4 and 5 of aeromonas caviae w-61.aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5 [nguyen, v. d. et al., biosci. biotechnol. biochem., 56, 1708-1712 (1993)]. here we purified and characterized high-molecular-weight xylanases, the xylanases 4 and 5 from the culture fluids of the bacterium. the purified xylanases 4 and 5, which had molecular masses of 120 and 140 kda, respectively, were endo-beta-1,4-xylanases with similar enzymatic properties except for trans-xylosidase activity. t ...200010737201
[isolation of aeromonas species from patients with sporadic diarrhea and characterization of aeromonas hydrophila isolates].a total of 16 strains of aeromonas species were isolated from feces of 348 patients with sporadic diarrhea in western kanagawa, japan from 1996 to 1998. of the 16 isolates, 7 were aeromonas hydrophila, 1 was a. sobria and 8 were a. caviae. the strains of a. hydrophila were examined for hemolytic activities, hemolysin gene types and o-serogroups. although all 7 strains of a. hydrophila showed hemolytic activities on sheep blood agar, in the test for hemolytic activities in culture supernatant, on ...200010741007
identification of motile aeromonas strains with the microscan walkaway system in conjunction with the combo negative type 1s panels.this study was performed to compare the microscan walkaway automated identification system in conjunction with the new microscan combo negative type 1s panels with conventional biochemical methods for identifying 85 environmental, clinical, and reference strains of eight aeromonas species.200010742279
overproduction of the secretin outd suppresses the secretion defect of an erwinia chrysanthemi outb mutant.outb is a component of the erwinia chrysanthemi out secretion machinery. homologues of outb have been described in two other bacteria, klebsiella oxytoca and aeromonas hydrophila, but their requirement in the secretion process seems to be different. study of outb topology with the blam topology probe suggests that it is an inner-membrane protein with a large periplasmic domain. however, fractionation experiments indicate that it could be associated with the outer membrane through its c-terminal ...200010746767
adventures of a pore-forming toxin at the target cell surface.the past three years have shed light on how the pore-forming toxin aerolysin binds to its target cell and then hijacks cellular devices to promote its own polymerization and pore formation. this selective permeabilization of the plasma membrane has unexpected intracellular consequences that might explain the importance of aerolysin in aeromonas pathogenicity.200010754575
aeromonas infection in acute suppurative cholangitis: review of 30 cases.aeromonads, though not common pathogens in biliary sepsis, caused substantial mortality in patients with impaired hepatobiliary function. our aim was to study the pathogenic role of aeromonas in acute suppurative cholangitis.200010762115
interaction of cefotetan and the metallo-beta-lactamases produced in aeromonas spp. and in vitro activity.aeromonas spp. are increasingly being recognized as human pathogens. the presence of metallo-beta-lactamases in these organisms represents a potential problem in antimicrobial therapy. mechanism-based inactivators of beta-lactamases are used to overcome the resistance of clinical pathogens to beta-lactam antibiotics, but no clinical useful inhibitors of the metallo-beta-lactamases are presently known. studying the interaction between cefotetan and aeromonas spp. producing metallo-beta-lactamase ...200010765032
amonabactin-mediated iron acquisition from transferrin and lactoferrin by aeromonas hydrophila: direct measurement of individual microscopic rate constants.the effectiveness and mechanism of iron acquisition from transferrin or lactoferrin by aeromonas hydrophila has been analyzed with regard to the pathogenesis of this microbe. the ability of a. hydrophila's siderophore, amonabactin, to remove iron from transferrin was evaluated with in vitro competition experiments. the kinetics of iron removal from the three molecular forms of ferric transferrin (diferric, n- and c-terminal monoferric) were investigated by separating each form by urea gel electr ...200010766437
the cytotoxic enterotoxin of aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages.an aerolysin-related cytotoxic enterotoxin (act) of aeromonas hydrophila possesses multiple biological activities, which include its ability to lyse red blood cells, destroy tissue culture cell lines, evoke a fluid secretory response in ligated intestinal loop models, and induce lethality in mice. the role of act in the virulence of the organism has been demonstrated. in this study, we evaluated the potential of act to induce production of proinflammatory cytokines associated with act-induced ti ...200010768977
characterization of the metallo-beta-lactamase determinant of acinetobacter baumannii ac-54/97 reveals the existence of bla(imp) allelic variants carried by gene cassettes of different phylogeny.the metallo-beta-lactamase determinant of acinetobacter baumannii ac-54/97, a clinical isolate from italy that was previously shown to produce an enzyme related to imp-1, was isolated by means of a pcr methodology which targets amplification of gene cassette arrays inserted into class 1 integrons. sequencing revealed that this determinant was an allelic variant (named bla(imp-2)) of bla(imp) found in japanese isolates and that it was divergent from the latter by 12% of its nucleotide sequence, w ...200010770756
interactions of streptomyces griseus aminopeptidase with a methionine product analogue: a structural study at 1.53 a resolution.sgap is an aminopeptidase present in the extracellular fluid of streptomyces griseus cultures. it is a double-zinc enzyme with a strong preference for large hydrophobic amino-terminus residues. it is a monomeric (30 kda) heat-stable enzyme, with a high and efficient catalytic activity modulated by calcium ions. the small size, high activity and heat stability make sgap a very attractive enzyme for various biotechnological applications. only one other related aminopeptidase (aeromonas proteolytic ...200010771423
molecular analysis of genetic differences between virulent and avirulent strains of aeromonas hydrophila isolated from diseased fish.aeromonas hydrophila, a normal inhabitant of aquatic environments, is an opportunistic pathogen of a variety of aquatic and terrestrial animals, including humans. a. hydrophila ppd134/91 is defined as virulent whereas ppd35/85 is defined as avirulent on the basis of their different ld50 values in fish. suppression subtractive hybridization (ssh) was used to identify genetic differences between these two strains. sixty-nine genomic regions of differences were absent in ppd35/85, and the dna seque ...200010784058
the pseudomonas group as an indicator of potential regrowth in water distribution systems.the regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (bdoc). the low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the pseudomonadaceae family, as this group can grow better at low concentrations of substrate than other bacteria also present in the distribution system. although the genus aeromonas has already been adopted as an indicator of this potential regrowth ...200010792530
evaluation of the environmental impact of microbial aerosols generated by wastewater treatment plants utilizing different aeration systems.using three sampler devices (sas, andersen six-stages and all glass impinger), the environmental impact of bacterial and fungal aerosols generated by municipal wastewater treatment plants operating with different methods of sludge oxygenation were evaluated. the highest microbial concentrations were recovered above the tanks (2247 cfu m-3) and in downwind positions (1425 cfu m-3), where a linear correlation (p < 0.05) was found between the quantity of sewage treated and the entities of microbial ...200010792545
interaction between aeromonas veronii and epithelial cells of spotted sand bass (paralabrax maculatofasciatus) in culture.an in vitro fish model to study the interaction between aeromonas veronii and skin, gill and intestinal epithelial cells was developed using primary cultures of mucosal cells (isolated from healthy organisms). primary cultures were exposed to aeromonas veronii strain a186 isolated from a patient with severe gastrointestinal disease. microbial adherence was assessed by a spectrophotometric evaluation of an enzyme-linked, biotin-streptavidin aer. veronii cell-adhesion assay to confluent monolayers ...200010792551
aeromonas wound infection in burns.infection of burn patients with the aeromonas organism is an uncommon event. this paper documents four cases of aeromonas hydrophila and one case involving both a. hydrophila and a. caviae occurring in burn patients between 1990 and 1998 at the royal brisbane hospital burns unit. the organism was isolated from either skin swabs, tissue samples, blood cultures or cultured lines. in all patients there was a history of immersion in water immediately post burn. there is one case of invasion and dest ...200010812272
a comparison of solid and liquid media for resuscitation of starvation- and low-temperature-induced nonculturable cells of aeromonas hydrophila.like many other gram-negative bacteria, starved cells of aeromonas hydrophila can be induced into a viable but nonculturable (vbnc) state by incubation at low temperature, as shown here by using various bacterial enumeration methods. starved a. hydrophila strain hr7 cells at 4 degrees c reached the nonculturable stage in about 45 days. the cells were resuscitated by either a solid medium resuscitation method, using solid agar amended with h2o2-degrading agents, catalase or sodium pyruvate, or a ...200010816051
enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a swedish clinic for infectious diseases.a 1-year prospective study was conducted to identify enteropathogens in adults with diarrhea (n=851) and in healthy control subjects (n=203) by use of conventional laboratory methods. virulence factor genes for diarrheagenic escherichia coli were detected by polymerase chain reaction. enteropathogens were identified in 56% of patients and 16% of control subjects. the isolation rate was 65% for patients with symptoms for <1 week and for travelers; >1 pathogen was found in 11% of patients. the mos ...200010816147
a major secreted elastase is essential for pathogenicity of aeromonas hydrophila.aeromonas hydrophila is an opportunistic pathogen and the leading cause of fatal hemorrhagic septicemia in rainbow trout. a gene encoding an elastolytic activity, ahyb, was cloned from aeromonas hydrophila ag2 into puc18 and expressed in escherichia coli and in the nonproteolytic species aeromonas salmonicida subsp. masoucida. nucleotide sequence analysis of the ahyb gene revealed an open reading frame of 1,764 nucleotides with coding capacity for a 588-amino-acid protein with a molecular weight ...200010816468
matrix-assisted laser desorption/ionization time-of-flight mass spectrometric detection of bacterial biomarker proteins isolated from contaminated water, lettuce and cotton cloth.matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectra of bacterial proteins were obtained from water, lettuce and cloth samples contaminated with shigella flexneri, escherichia coli, and aeromonas hydrophila. spectra were obtained using proteins directly isolated from water (or water used for rinsing samples) without culturing the bacteria. for s. flexneri and e. coli, two marker ions for specific proteins associated with a virulence-related property (acid resistanc ...200010825256
[septic arthritis caused by aeromonas hydrophila]. 200010827789
bacteraemia caused by non-glucose-fermenting gram-negative bacilli and aeromonas species in patients with haematological malignancies and solid tumours.the clinical characteristics and outcome of bacteraemia caused by non-glucose-fermenting gram-negative bacilli and aeromonas spp. were examined in 115 adults with haematological malignancies or solid tumours. the most aggressive pathogens were pseudomonas aeruginosa, stenotrophomonas maltophilia, aeromonas spp., acinetobacter spp. and burkholderia cepacia, all of which caused either septic syndrome or pneumonia in more than 40% of cases. pseudomonas aeruginosa was involved less often in catheter ...200010834826
primary pseudomonas meningitis in an adult, splenectomized, multitransfused thalassaemia major patient.a 19-year-old splenectomized, multitransfused female patient with beta-thalassaemia major developed primary meningitis due to p. putida. her blood cultures were negative. p. putida is an unusual nosocomial organism to cause primary meningitis. infection due to this organism carries high mortality. however, owing to early diagnosis and energetic treatment this patient survived without any sequelae. a review of serious infections over the last 7 years in patients in our thalassaemia care centre re ...200010841329
aeromonas salmonicida subsp. pectinolytica subsp. nov., a new pectinase-positive subspecies isolated from a heavily polluted river.aeromonas strains which phenotypically and genetically belong to the aeromonas salmonicida species but that according to their phenotypic properties constitute a new subspecies have been isolated from the water of a heavily polluted river, the matanza river, situated near the central district of buenos aires city. these strains were ascribed to the a. salmonicida species by using 65 biochemical tests and by dna-dna hybridization. they produce acid from -sorbitol, an unusual biochemical property ...200010843053
virulence properties of motile aeromonads isolated from farmed frogs rana tigerina and r. rugulosa.virulence factors were compared in aeromonas species isolated from clinically normal and septicaemic farmed frogs from thailand. haemolysin activities against frog erythrocytes were significantly different within the collection of aeromonads. groups of high haemolytic activity (unspeciated aeromonas, au), moderate haemolytic activity (a. hydrophila), and low haemolytic activity (a. veronii biovar sobria, a. veronii biovar veronii, a. caviae, a. schubertii) were noted. dna colony hybridisation st ...200010843556
the cardiovascular hemodynamics and leukotriene kinetics during prostacyclin and anti-prostacyclin antibody infusions in septic shock.this study evaluated whether or not prostacyclin (pgi2) was necessary or sufficient by itself in a pathophysiologic concentration to mediate the cardiovascular dysfunction of septic shock. anesthetized adult swine received anesthesia only (anesthesia control, n = 6); graded aeromonas hydrophila, 10(10)/ml, infusion at 0.2 ml/kg/h that increased to 4.0 ml/kg/h over 3 h (septic shock control, n = 6); pathophysiologic prostacyclin infusion to match septic shock control plasma levels without bactere ...200010847636
survey for psychrotrophic bacterial pathogens in minimally processed lettuce.a total of 120 minimally processed, cut and packaged lettuce samples were purchased from retail supermarkets or provided by a salad production facility over an 8-month period. the samples were tested for total aerobic plate counts and for the presence of potentially pathogenic species belonging to the genera of listeria, aeromonas and yersinia. the aerobic plate counts ranged from 103 to 109 colony forming units (cfu) g-1. most samples (76%) contained between 105 and 107 cfu g-1 total aerobic ba ...200010849276
anti-adhesive activity of sulphated exopolysaccharides of microalgae on attachment of red sore disease-associated bacteria and helicobacter pylori to tissue culture cells.because of the affinity of certain bacterial species for sulphated glycoconjugates exposed on the epithelial cells of susceptible hosts, we hypothesized that sulphated exopolysaccharides of microalgae can be used in anti-adhesive therapies against bacterial infections, both in cold- and warm-blooded animals. in this study we found that adhesion of the human pathogen helicobacter pylori to the hela s3 cell line, and adhesion of the fish pathogens vibrio campbellii, v. ordalii, streptococcus sapro ...200010849279
antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation.the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ...200010852568
[immunoenzyme method for detecting microbial producers of palytoxin].a competitive elisa using the intact toxin as a coating antigen for detecting palytoxin was developed. this immunoassay allows palytoxin (ptx) to be determined in the range of 6-250 ng/ml. in sensitivity, this determination is comparable with ria but is three times inferior to elisa using monoclonal antibodies. inhibition experiments using some toxins of marine invertebrates proved the serological specificity of the palytoxin binding to antibodies. both the indirect and competitive elisa were us ...200010857024
investigation of the role of type iv aeromonas pilus (tap) in the pathogenesis of aeromonas gastrointestinal infection.although there is substantial evidence that type iv pili purified from diarrhea-associated aeromonas species (designated bfp for bundle-forming pilus) are intestinal colonization factors (s. m. kirov, l. a. o'donovan, and k. sanderson, infect. immun. 67:5447-5454, 1999), nothing is known regarding the function of a second family of aeromonas type iv pili (designated tap for type iv aeromonas pilus), identified following the cloning of a pilus biogenesis gene cluster tapabcd. related pilus gene c ...200010858220
typing of clinical and environmental aeromonas veronii strains based on the 16s-23s rdna spacers.genetic relationships of aeromonas veronii strains isolated from human and environmental sources were investigated by restriction fragment length polymorphism (rflp) of the polymerase chain reaction-amplified intergenic spacer region (isr) flanked by the 16s and 23s rrna genes. when using endonucleases alui, hinfi and cfoi the 16s-23s rdna-rflp patterns showed considerable overall similarity, although most strains yielded specific profiles. several intra-specific lines of descent comprised clini ...200010865175
use of restriction fragment length polymorphism of the pcr-amplified 16s rrna gene for the identification of aeromonas spp. 200010866546
modification of the bile salts-irgasan-brilliant green agar for enumeration of aeromonas species from food.the present study evaluated the productivity of bibg medium for the isolation of aeromonas spp. from food and describes a modification of the bibg medium (mbibg) (increased ph (8.7), replacement of xylose by soluble starch as a carbon source, decreased concentration of bile salts) to increase its selectivity and electivity. using the mbibg medium, growth of the majority of the enterobacteriaceae (9/10) was suppressed except for citrobacter freundii. the mbibg medium supported growth of pseudomon ...200010868682
clinically relevant aeromonas species. 200010880331
acute renal failure in an infant associated with cytotoxic aeromonas sobria isolated from patient's stool and from aquarium water as suspected source of infection. 200010681210
[physico-chemical and microbiological characteristics of groundwater from observation boreholes of a deep radioactive liquid waste repository].a radioactive liquid waste repository was found to be a habitat of the rich microbial community with a high catabolic potential. groundwater from a depth of 162-189 m contained aerobic saprotrophic and anaerobic fermentative, sulfate-reducing, and denitrifying bacteria. nitrate-reducing bacteria residing in this groundwater were isolated in pure cultures. based on the results of their physiological studies, 16s rrna sequencing, and phylogenetic analysis, the microorganisms isolated were ascribed ...200010808497
cloning, sequencing and expression of the gene encoding the extracellular metalloprotease of aeromonas caviae.a gene (apk) encoding the extracellular protease of aeromonas caviae ae6 has been cloned and sequenced. for cloning the gene, the dna genomic library was screened using skim milk lb agar. one clone harboring plasmid pkk3 was selected for sequencing. nucleotide sequencing of the 3.5 kb region of pkk3 revealed a single open reading frame (orf) of 1,785 bp encoding 595 amino acids. the deduced polypeptide contained a putative 16-amino acid signal peptide followed by a large propeptide. the n-termin ...200010888351
kombucha fermentation and its antimicrobial activity.kombucha was prepared in a tea broth (0.5% w/v) supplemented with sucrose (10% w/v) by using a commercially available starter culture. the ph decreased steadily from 5 to 2.5 during the fermentation while the weight of the "tea fungus" and the od of the tea broth increased through 4 days of the fermentation and remained fairly constant thereafter. the counts of acetic acid-producing bacteria and yeasts in the broth increased up to 4 days of fermentation and decreased afterward. the antimicrobial ...200010888589
rattus norvegicus: not a model for aeromonas-associated gastroenteritis in man.the lack of a suitable animal model of aeromonas-associated diarrhoea has hampered investigations into aeromonas pathogenic mechanisms. hence, a published report that clindamycin-pretreated rats developed signs and symptoms of enteritis following intragastric inoculation of an aeromonas strain required further investigation. although we could demonstrate long-term colonisation (>12 days) and histological damage in this animal model with pseudomonas aeruginosa isolated from patients with chronic ...200010891655
assessment of the microbiological quality and wash treatments of lettuce served in university restaurants.one hundred and forty-four samples of lettuce from 16 university restaurants were analyzed. the mesophilic aerobic counts of all samples ranged from 3.01 to 7.81 log10 cfu g(-1). results of total coliforms ranged from < 0.47 to > 3.38 log10 most probable number (mpn) g(-1). of the lettuce samples, 25.7% harbored escherichia coli, 22.9% staphylococcus aureus and 84% group d streptococci. similarly, 10.4% of the samples harbored aeromonas hydrophila, 2.8% pseudomonas aeruginosa, and coliforms such ...200010898470
necrotizing fasciitis caused by aeromonas hydrophila.aeromonas hydrophila is a gram-negative bacillus commonly found in soil, sewage, and fresh or brackish water in many parts of the united states. in healthy people, the most common clinical manifestations attributed to aeromonas are diarrhea and soft tissue infections. in people with suppressed immune systems or liver disease, a hydrophila can cause meningitis, endocarditis, peritonitis, hemolytic-uremic syndrome, or septicemia. we present the first known case of fulminant necrotizing fasciitis f ...200010900069
clinical features and therapeutic implications of 104 episodes of monomicrobial aeromonas bacteraemia.aeromonas bacteraemia is not a common infectious disease, but can cause a grave outcome in infected cases. in this study, clinical presentations and prognostic factors of cases of monomicrobial aeromonas bacteraemia were analysed. also, the impact of beta-lactam and aminoglycoside in combination and of emerging cephalosporin-resistance during therapy was discussed.200010908022
corrigendum to: kinetic and spectroscopic characterization of native and metal-substituted beta-lactamase from aeromonas hydrophila ae036 (febs 23250). 200010908738
acute community-acquired diarrhea requiring hospital admission in swiss children.in order to ascertain the prevalence of agents that cause childhood diarrheal illness, stool specimens of 312 consecutive children with community-acquired diarrhea requiring admission were evaluated. pathogens were detected in 166 (53%) of the 312 children (>/=2 pathogens in 28 children): rotavirus (n=75), salmonella spp. (n=37), campylobacter spp. (n=24), shigella spp. (n=5), giardia spp. (n=4), yersinia spp. (n=2), aeromonas spp. (n=15), cryptosporidium (n=15), enteropathogenic escherichia col ...200010913424
isolation of aeromonas hydrophila in children with diarrhea.aeromonas species are gram-negative bacilli that lead to a wide spectrum of infectious diseases in human. in the present study, these bacilli were reviewed to assess their significance as enteric pathogens in this area. during the years of 1994 to 1998, the clinical microbiology laboratory received 2150 stool specimens of children with diarrhea. medical charts of patients who had positive culture for aeromonas hydrophila were reviewed. a. hydrophila were isolated from 50 (2.3%) of 2150 children ...200010917882
isolations of enteric pathogens from synanthropic flies trapped in downtown kuala lumpur.four species of synanthropic flies were trapped in downtown kuala lumpur: chrysomya megacephala, chrysomya rufifacies, musca domestica, and musca sorbens. burkholderia pseudomallei, the organism causing melioidosis, was the dominant bacteria isolated from chrysomya megacephala. klebsiella oxytoca, commonly associated with nosocomial infections, was commonly isolated from chrysomya megacephala, musca domestica, and musca sorbens. aeromonas hydrophila, the bacteria causing gastroenteritis, was pre ...200010925800
rifampicin sub-inhibitory concentrations reduce the expression of haemolysin in aeromonas spp. 200010926450
an outbreak of acute gastroenteritis due to aeromonas sobria in benghazi, libyan arab jamahiriya.we report an outbreak of acute diarrhoea due to aeromonas sobria in benghazi which occurred during a 1-month period in 1997. of 69 patients admitted with acute gastroenteritis, 28 were positive for a. sobria based on the production of gas from glucose, the production of acetoin, hydrogen sulfide and lysine decarboxylase and on aesculin hydrolysis and fermentation of arabinose and salicin. the strains were sensitive to chloramphenicol, co-trimoxazole, tetracycline and gentamicin but resistant to ...200011556043
l-asparaginase activity in aeromonas sp. isolated from freshwater mussel.aeromonas sp. from lamellidens marginalis produced l-asparaginase when grown at 37 degrees c. the optimum enzyme activity was at ph 9 when temperature was 45 degrees c. half-life of partially purified enzyme at 50 degrees c and 55 degrees c was 35 and 20 min, respectively. activation and deactivation energies of partially purified enzyme were 17.48 and 24.86 kcal mol-1 respectively. the enzyme exhibited a km (l-asparagine) value of 4.9 x 10(-6) mol l-1 and a vmax of 9.803 iu ml-1. three metal io ...200011395960
prevalence of enterotoxigenic motile aeromonads in children, fish, milk and ice-cream and their public health significance.motile aeromonads, which have been implicated as causative agents of human gastroenteritis, meningitis. endocarditis and osteomyelitis, were isolated from fish, pasteurized and flavored milk and ice creams. forty-five isolates of aeromonas from diarrheic children (100) and 285 food samples comprising of fish (100), milk (85) and ice creams (100) were identified as a. hydrophila (21), a. sobria (16) and a. caviae (8). five of these 45 isolates of aeromonoas (3 a. sobria, 2 a. hydrophila) were fro ...200011414446
mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand.chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ...200011414447
occurrence of enterotoxigenic aeromonas species in foods.out of a total of 246 food samples of animal origin screened for isolation of aeromonas spp., 33 (13.41%) were positive for these organisms. maximum positivity was shown by the samples from fish (28.57%), followed by poultry meat (16.67%), poultry eggs (12.50%), goat meat (12%), buffalo meat (7.69%) and cow milk (5.56%). a. hydrophila was the predominant species (51.52%) followed by a. sobria (39.39%) and a. caviae. of these, 70.59% a. hydrophila, 69.23% a. sobria and 33.33% a. caviae showed ent ...200011407001
outcomes of aeromonas bacteremia in patients with different types of underlying disease.over a 6-year period, 42 patients with different underlying diseases developed aeromonas bacteremia in our hospital. the male to female ratio was 2:1. the vast majority of these patients had underlying diseases, including various types of neoplasm (n = 14), liver cirrhosis (n = 11), biliary tract disorder (n = 10) and other illnesses (n = 7). community-acquired bacteremia was predominant (33 cases, 79%). aeromonas hydrophila was the most common species isolated (88%). monomicrobial bacteremia wa ...200011269369
[pathogenicity factors of various vibrios and aeromonads].a review of literature on the pathogenicity factors of non-cholera vibrios and aeromonas is presented. a detailed analysis of such pathogenicity factors as hemolysins, lecithinases, cytotoxins, adhesins, etc. is given. information on some mechanisms of the pathogenetic action of these factors on warm-blooded animals is presented. the necessity of more extensive research on pathogenicity and persistence factors in opportunistic bacteria causing sapronotic infections is emphasized.200011210617
aeromonas caviae as a cause of cholecystitis. 200011217277
[microbiological monitoring of causative agents of sapronoses in the water of the bogatinskoe water reservoir].the results of the microbiological monitoring of potential causative agents of sapronoses in the water of the bogatinskoye reservoir revealed that in the summer period of 1998 the mass accumulation of virulent aeromonas sobria (up to 25% of the total number of heterotrophic bacteria) took place. the autumn period was characterized by a decrease in the number of a. sobria and the detection of bacteria of the genus vibrio (up to 22%) with v. mimicus and v. metschnikovii identified among them in th ...200011220958
identification and characterization of carnobacteria associated with the gills of atlantic salmon (salmo salar l.).using the surface plate technique, the population level of aerobic bacteria, occurring in the gills of atlantic salmon (salmo salar l.), was determined to be approximately 3 x 10(4) g(-1). of 100 isolates investigated, 58 were gram-negative. out of the 42 gram-positive isolates, 26 belonged to the carnobacteria, of which ten were further identified on the basis of 16s rdna sequence analysis and aflp fingerprinting. all were identified as carnobacterium piscicola-like. these carnobacteria strains ...200011249022
characterization of oxytetracycline-resistant heterotrophic bacteria originating from hospital and freshwater fishfarm environments in england and ireland.this ecotaxonomic study compared the antibiotic tolerance among culturable oxytetracyline-resistant (ot(r)) heterotrophic strains isolated from two aquatic environments representing human activities in health care and aquaculture, namely hospital effluents and freshwater fishfarms. using a standardized methodology, samples taken in england and ireland were analyzed to determine the antibiotic tolerance profiles of two groups of culturable ot(r) bacterial isolates at the intergeneric and intragen ...200011249032
the spontaneous haemolytic activity of cod serum: heat insensitivity and other characteristics. 200011185757
rapidly progressive pneumonia due to aeromonas hydrophila shortly after near-drowning.an 87-year-old woman died of rapidly progressive pneumonia due to aeromonas hydrophila shortly after a near-drowning event. autopsy showed necrotizing pneumonia and postmortem cultures of both blood and lung revealed the organism. fulminant pneumonia should be considered in patients of a near-drowning event.200011197807
production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of aeromonas hydrophila.the newly screened aeromonas hydrophila produces copolymer consisting of 3-hydroxybutyrate (3hb) and 3-hydroxyhexanoate (3hhx). the characteristics of cell growth and polymer accumulation were examined using various carbon sources. p(3hb-co-3hhx) was produced from lauric acid and oleic acid only. p(3hb-co-3hhx) content can be increased by limitation of phosphorus. a maximal p(3hb-co-3hhx) content of 28.8 wt% could be obtained in flask culture. by applying the optimally designed nutrient feeding ...200010592522
dna probes specific for aeromonas hydrophila (hg1).aeromonas hydrophila (hg1)-specific rapd-pcr fragments were investigated for their potential as dna probes. from 20 rapd-pcr fragment bands, it was found that two were specific to all isolates of aeromonas hydrophila (hg1) tested. cloning and nucleotide sequence determination of one of these bands showed that co-migration of similar sized amplicons had occurred and that this band (designated '7e') contained at least four fragments of different sequences. three of these individual amplicons had a ...199910063616
monitoring the conjugal transfer of plasmid rp4 in activated sludge and in situ identification of the transconjugants.a gfpmut3b-tagged derivative of broad host-range plasmid rp4 was used to monitor the conjugative transfer of the plasmid from a pseudomonas putida donor strain to indigenous bacteria in activated sludge. transfer frequencies were determined to be in the range of 4 x 10(-6) to 1 x 10(-5) transconjugants per recipient. in situ hybridisation with fluorescently labeled, rrna-targeted oligonucleotides was used to phylogenetically affiliate the bacteria that had received the plasmid.199910234817
distribution of two hemolytic toxin genes in clinical and environmental isolates of aeromonas spp.: correlation with virulence in a suckling mouse model.previous studies have shown that two hemolytic toxins, hlya and aera, contribute to the virulence of aeromonas hydrophila. a survey was performed to gauge the distribution of hlya and aera genes in clinical and environmental aeromonas isolates. for a. hydrophila, a. veronii biotype sobria and a caviae, 96%, 12% and 35% of strains, respectively, were hlya positive, whereas, 78%, 97%, 41%, respectively, were aera positive. all virulent a. hydrophila isolates were hlya+ aera+. this genotype was mos ...199910234831
unusual case of aeromonas sobria cellulitis associated with the use of leeches. 199910192721
evaluation of xylose-galactosidase medium, a new plate for the isolation of salmonella, shigella, yersinia and aeromonas species. 199910192724
effect of florfenicol on the immune response of rainbow trout (oncorhynchus mykiss).florfenicol, a drug effective against several bacterial diseases of fish, was tested for possible immunomodulatory effects. the aim of the study was to follow the kinetics of the immune response after vaccination with simultaneous oral antibiotic treatment. the fish were immunised with a commercial oil-based divalent (furunculosis/vibriosis) vaccine and were simultaneously given oral antibiotic treatment. the specific immune response was monitored by analysing the levels of specific antibodies w ...199910206200
identification of aeromonas trota (hybridization group 13) by amplification of the aerolysin gene using polymerase chain reaction.aeromonas trota is recognized as an important enteropathogen, and its haemolysin (aerolysin) is purported to be one of the virulence factors. rapid detection and identification of a. trota is important for early and specific diagnosis of the infectious diseases that it causes. synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) technique to amplify a species-specific sequence of the aera gene, which encodes the aerolysin of a. trota. a dna fragment of 622 bp was ampl ...199910208799
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