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a 70 kda aeromonas salmonicida serine protease - beta-galactosidase hybrid protein as an antigen and its protective effect on atlantic salmon (salmo salar l.) against a virulent a. salmonicida challenge. 19938449332
[the pattern of the sensitivity of aeromonas spp. that produce extraintestinal infections].we studied the sensibility to several antimicrobics of aeromonas strains isolated in extraintestinal infections during a period of five years, in order to establish which could be the best empirical therapy in these processes. twenty-two strains of a. hydrophila were evaluated, was isolated (8 from hemocultures, 6 from wound exudate, 2 from abscess aspirate, 2 from peritoneal fluid and 1 from urine, pleural fluid, bile and catheter). the identification was made using the gni card system (vitek s ...19938452974
[the amine-forming ability of aeromonas spp].motile aeromonas (a.) species are considered international more and more as potential food poisoning organisms. their ability to produce biogenic amines, products of metabolism, which in case can cause a disease, was only searched insufficiently till now. 50 strains of the species a.hydrophila, a.sobria, a.caviae and the non-motile species a.salmonicida were included in the tests for amine producing potency. qualitative investigations to the formation of histamine, tryptamine, and tyramine were ...19938456569
in vitro activity of cefdinir (fk 482, pd 134393, ci-983): a new orally active cephalosporin.cefdinir is a new orally active cephalosporin which is undergoing in vitro and in vivo evaluations. using the standard agar dilution method we compared the in vitro activity of this drug with other beta-lactam antibiotics against clinical isolates or enterobacteriaceae (625 strains), pseudomonas aeruginosa (68 strains), xanthomonas maltophilia (36 strains), acinetobacter (52 strains), aeromonas hydrophilia (47 strains), staphylococci (364 strains) and enterococci (50 strains). against most membe ...19938458243
high-affinity binding of the basement membrane protein collagen type iv to the crystalline virulence surface protein array of aeromonas salmonicida.the surface of the fish pathogen aeromonas salmonicida is covered by a paracrystalline array (the a-layer) which is a virulence factor for the organism. quantification of the ability of a. salmonicida cells to bind collagen types i and iv in a 125i-radiolabelled liquid-phase assay showed that a-layer-positive cells bound high levels of collagen type iv, but significantly lower levels of collagen type i. collagen type iv binding was confirmed using non-radiolabelled enzyme-linked immunosorbent as ...19938459775
microbial flora of frozen tail meat from captive nile crocodiles (crocodylus niloticus).frozen tail meat samples from nine nile crocodiles (crocodylus niloticus) were evaluated for microbial numbers and types. microbial analyses yielded a mean aerobic plate count (apc, 30 degrees c) of 4.86 log cfu/g, a mean coliform count (tc, 37 degrees c) of 3.77 log cfu/g and a mean faecal coliform count (fc, 44 degrees c) of 3.40 log cfu/g. salmonella was isolated from three samples, and beta-haemolytic streptococci from two samples. yersinia, aeromonas, staphylococcus aureus and bacillus cere ...19938466815
an overview of the kinetic parameters of class b beta-lactamases.the catalytic properties of three class b beta-lactamases (from pseudomonas maltophilia, aeromonas hydrophila and bacillus cereus) were studied and compared with those of the bacteroides fragilis enzyme. the a. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. no relationships were found between sequence similarities and catalytic properties. the problem of the repartition of class b beta-lactamases into sub-classes is ...19938471035
adherence to hep-2 cells and enteropathogenic potential of aeromonas spp.aeromonas strains (total = 60) of clinical, water and food origin were tested for adherence to hep-2 cells. environmental strains were selected (except for a. caviae) to include primarily those expressing other virulence-associated properties. adhesion was markedly species-dependent (a. veronii biotype sobria, 15 of 26 [58%]. a caviae, 4 of 12 [33%] and a. hydrophila, 2 of 8 [11%]). a. veronii biotype sobria were adhesive, irrespective of source (62 and 54% for clinical and environmental strains ...19938472772
purification and characterization of a novel zinc-proteinase from cultures of aeromonas hydrophila.while searching for an enzyme capable of breaking epsilon-(gamma-glu)-lys isopeptide bonds cross-linking protein chains, we purified a metallo-proteinase which mimics the action of an isopeptidase on the gamma-chain dimers of cross-linked fibrin. the enzyme is present in the growth medium of the bacterium aeromonas hydrophila, isolated from the intestinal tract of the leech hirudo medicinalis. it is a 19-kda protein which specifically hydrolyzes the gly-ala peptide bond within the gly-gly-ala se ...19938473348
ctx genetic element encodes a site-specific recombination system and an intestinal colonization factor.in vibrio cholerae, the genes encoding cholera toxin (ctxab) are located on a segment of dna (termed the "core" region) that is flanked by two or more copies of a repeated sequence called rs1. together these dna units comprise the ctx genetic element. evidence presented here suggests that rs1 sequences encode a site-specific recombination system, which allows integration of a suicide plasmid carrying rs1 into an 18-base-pair sequence (attrs1) located on the chromosome of nontoxigenic v. cholerae ...19938475125
an aromatic-dependent mutant of the fish pathogen aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis.aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. the disease is responsible for severe economic losses in intensively cultured salmon and trout. bacterin vaccines provide inadequate protection against infection. we have constructed an aromatic-dependent mutant of a. salmonicida in order to investigate the possibility of an effective live-attenuated vaccine. the aroa gene of a. salmonicida was cloned in escherichia coli, and the nucleotide sequence was determined. ...19938478107
survival of nonculturable aeromonas salmonicida in lake water.the survival of aeromonas salmonicida subsp. salmonicida was investigated in sterile and untreated lake water. in sterile lake water (filtered and autoclaved), it was found that cells of a. salmonicida entered a nonculturable but viable condition. viability was determined by flow cytometry with the dye rhodamine 123, which is taken up and maintained within cells with a membrane potential. for survival studies in untreated lake water, a. salmonicida was marked with the xyle gene by using the plas ...19938481011
[efficacy of cefsulodin -irgasan-novobiocin agar media in the isolation of aeromonas mesophiles from feces]. 19938481436
[incidence, behavior and control of aeromonas hydrophila in meat and dairy products].this review deals with several aspects of aeromonas hydrophila and other motile aeromonas species associated with foodborne illness. although it is mainly dedicated to the factors affecting growth and survival of this species in foods of animal origin, information on other topics is also provided. this paper includes sections on: taxonomy, diseases caused by aeromonas, virulence factors, reservoirs and prevalence in foods and water, factors affecting growth and survival, isolation and identifica ...19938484917
identification of aeromonas schubertii and aeromonas jandaei by using a polymerase chain reaction-probe test.two oligonucleotide primers were used in a polymerase chain reaction-protocol to amplify a region (approx. 850 bp) of the 16s rrna gene of aeromonas schubertii and aeromonas jandaei. hybridization of the polymerase chain reaction products to specific internal probes provided a highly specific method for the identification of these two species.19938486241
production and specificity of poly- and monoclonal antibodies raised against shewanella putrefaciens.polyclonal antibodies were raised in rabbits and mice against shewanella putrefaciens. murine monoclonal antibodies were produced against the type strain (atcc 8071) as well as wild type strains isolated from fish products. the specificities of four polyclonal and 12 monoclonal antibodies were tested by dot-blotting, an indirect and a competitive elisa against 16 gram-negative strains; including six strains of s. putrefaciens and one strain of pseudomonas rubescens (nc 10695). all polyclonal ant ...19938486552
an epizootic of edwardsiella tarda in largemouth bass (micropterus salmoides).edwardsiella tarda, an opportunistic bacterial pathogen, was isolated from dying largemouth bass (micropterus salmoides) during an epizootic in a eutrophic lake system, lochloosa lake, florida, usa. approximately 1,500 adult fish died over a 6-wk period during the late summer and early fall of 1991. a mixed population of aerobic bacteria (e. tarda, aeromonas hydrophila, and pseudomonas sp.) was isolated from deep cutaneous ulcers and intestines of moribund bass. however, e. tarda in pure culture ...19938487385
impact of the ring-billed gull (larus delawarensis) on the microbiological quality of recreational water.we evaluated the impact of the ring-billed gull (larus delawarensis) on the microbiological quality of water. we measured fecal coliforms, salmonella spp., and aeromonas spp. in the gull droppings and the number of fecal coliforms in the water prior to and after attracting these birds to the beach with food. gulls can contribute to the bacteriological degradation of recreational water.19938489231
three-year prevalence of enteropathogenic bacteria in an urban patient population in germany.the prevalence of enteropathogenic bacteria over a three-year period was 3.1% in an urban population in germany. nontyphoidal salmonella spp. (1.8% prevalence) represented 59.3% of all positive isolates, followed by campylobacter spp., which constituted 22.1% of such isolates. clostridium difficile toxin was detected in 5.6% of samples submitted specifically for detection of cytotoxigenic c. difficile. the seasonal distribution showed an increase of salmonella, shigella and aeromonas/plesiomonas ...19938491518
an aeromonas salmonicida gene which influences a-protein expression in escherichia coli encodes a protein containing an atp-binding cassette and maps beside the surface array protein gene.a conserved aeromonas salmonicida gene (abca) affecting expression of the surface array protein gene (vapa) in escherichia coli was identified. the 924-bp gene starts 205 bp after vapa and codes for a protein with a deduced molecular weight (m(r)) of 34,015 containing an n-terminal p-loop and significant homology to the atp-binding cassette transport protein superfamily. abca was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) by using t7 polymerase expression ...19938491726
in vitro susceptibilities of tropical strains of aeromonas species from queensland, australia, to 22 antimicrobial agents.greater than 90% of 131 strains of aeromonas species were susceptible to the aminoglycosides, ureidopenicillins, extended-spectrum cephalosporins, aztreonam, quinolones, tetracycline, and chloramphenicol, and all were uniformly resistant to ampicillin. except for amoxicillin-clavulanate, sulfonamide, trimethoprim, and trimethoprim-sulfamethoxazole, there was good correlation between the results obtained by the agar dilution and disk diffusion techniques.19938494389
comparative antibacterial activity of the aminothiazolyl cephalosporin ru 29,246.a total of 1,007 clinical isolates from a tertiary care center were tested against ru 29,246, ampicillin, cephalothin, cefoxitin, ceftazidime, augmentin, oxacillin, piperacillin, gentamicin, amikacin and vancomycin. bacteria tested consisted of 479 strains of enterobacteriaceae, 64 pseudomonads, 18 xanthomonas, 42 other gram-negative bacilli, 56 enterococci and 348 isolates of staphylococci. ru 29,246 showed excellent in vitro activity inhibiting > 90% of escherichia coli, klebsiella pneumoniae, ...19938508687
spectrum of extraintestinal disease due to aeromonas species in tropical queensland, australia.during a 12-month period, the clinical spectrum of extraintestinal disease due to aeromonas species was determined for 56 patients in tropical queensland (australia). forty-six patients acquired their infection in the community, six patients were infected in the hospital, and four patients were colonized. demographic risk factors included male gender (67%) and aboriginal ethnic background (35%). the disease ranged from deep-seated infection (four cases) to soft-tissue infection of varying intens ...19938513068
extra-label drug use in aquaculture. 19938514576
diarrhea associated with aeromonas species in children in day care centers.outbreaks of diarrhea caused by enteropathogens have been reported in day care centers (dcc), but aeromonas species have not been implicated. this study evaluated 381 children involved in 51 outbreaks in four dcc to determine the association of aeromonas species with diarrhea and to characterize the isolates. the organism was identified in two outbreaks of diarrhea. in one, aeromonas species were isolated from 6 (24%) of 25 children and in the other from 5 (21%) of 24 children. seven other aerom ...19938515113
biochemical properties of a carbapenem-hydrolyzing beta-lactamase from enterobacter cloacae and cloning of the gene into escherichia coli.a clinical isolate of enterobacter cloacae, strain nor-1, exhibited resistance to imipenem and remained susceptible to extended-spectrum cephalosporins. clavulanic acid partially restored the susceptibility of the strain to imipenem. two beta-lactamases with isoelectric points (pi) of 6.9 and > 9.2 were detected in strain e. cloacae nor-1; the higher pi corresponded to ampc cephalosporinase. plasmid dna was not detected in e. cloacae nor-1 and imipenem resistance could not be transferred into es ...19938517720
[various new pathogens in pneumonia].the table summarizes the many newly recognized agents causing lower respiratory tract infections: gram + cocci, streptococcus agalactiae, enterococci; gram-rods, acinetobacter, aeromonas hydrophila, eikenella corrodens; mycobacterium tuberculosis resistant to multiple drugs, intracellular organisms and viruses. some details are given in four chapters: pneumococci resistant to penicillin g which must be detected and impose new therapeutic strategies; chlamydia pneumoniae with its difficulties for ...19958525335
metal content and conformation of the metalloprotease from the marine sponge spheciospongia vesparia.we have recently purified a protease from the marine sponge spheciospongia vesparia. it consists of a single nonglycosylated polypeptide chain with a molecular weight of 29 600 and has one free thiol group. metal analysis revealed the presence of zinc at 2.02 +/- 0.05 g-atoms per mole of protein, as measured by atomic absorption spectroscopy. the circular dichroism spectrum in the far uv region (183-259 nm) indicates that the sponge protease contains appreciable amounts of beta sheet. this enzym ...19958528145
sequence analysis of two chromosomally mediated inducible beta-lactamases from aeromonas sobria, strain 163a, one a class d penicillinase, the other an ampc cephalosporinase.two beta-lactamase genes from aeromonas sobria, strain 163a, have been cloned and sequenced, one encoding a typical class c cephalosporinase, designated ceps, the other a class d penicillinase, designated amps. ceps is predicted to be a mature protein of 38 kda with a pi value of 7.0. the amino acid sequence of ceps is most similar to that of ampc from pseudomonas aeruginosa (54.7%). amps is predicted to be a mature protein of 27 kda with a pi value of 7.9 that mostly closely resembles blad from ...19958537283
role of aeromonas isolated from feces of finnish patients.aeromonas species were isolated from 249/13,027 (1.9%) stool samples submitted to the dept. of bacteriology and immunology, university of helsinki, during 1 year, to be cultured for bacterial enteropathogens. aeromonas was the third most common enteropathogen isolated, after campylobacter (3.6%) and salmonella (3.3%). isolates and clinical information from 234 aeromonas patients were available for further study. a. caviae (41%), a. hydrophila (27%), and a. veronii biovar sobria (22%) were the mo ...19958539542
aeromonas hydrophila lung abscess in a previously healthy man.we present a case of multilocular lung abscess caused by aeromonas hydrophila, found in both sputum and bronchial washing. no aspiration or other significantly contributory medical conditions were found. we believe this to be the first report of lung abscess caused by this organism in a previously healthy individual.19958539557
aeromonas sobria infection with severe muscle degeneration in a patient with alcoholic liver cirrhosis.a 56-yr-old male who had been followed for alcoholic liver disease was admitted for abdominal pain and a high fever. gastrointestinal endoscopy revealed bleeding esophageal varices that were treated by endoscopic sclerotherapy. blood culture on admission was positive for aeromonas sobria. then skin bullas and ulcers and severe muscle degeneration developed. the patient died despite extensive treatment with antibiotics. a. sobria infection in patients with liver cirrhosis is rare.19958540527
purification and some properties of six chitinases from aeromonas sp. no. 10s-24.six chitinases were purified from a culture supernatant of aeromonas sp. no. 10s-24 by ammonium sulfate precipitation, deae-sephadex a-50, butyl-toyopearl 650m, and chromatofocusing. these enzymes were most active at ph 3.5-4.5 and the optimum temperature were 50 degrees c. the molecular weights of the enzymes were 89,000 to 120,000 from sds-polyacrylamide gel electrophoresis. n-terminal amino acid sequences of the enzymes were similar to that of chitinase i.19958541660
development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum.a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ...19958548693
presence of aeromonas trota in aquatic environment. 19958551948
treatment of aeromonas hydrophila infection in a deep tissue wound.we report the case of a young woman who sustained a knee laceration in an automobile accident and was subsequently exposed to water. although properly cleansed and closed, her wound became infected and she was initially treated with a single-dose parenteral cephalosporin. the infection progressed and cultures indicated a polymicrobial infection which included aeromonas hydrophila. significant tissue damage ensued and a lengthy hospitalization was required. aeromonas, although rarely a cause of i ...19958553050
fate of aeromonas and yersinia on modified-atmosphere-packaged (map) cod and trout.the growth/survival of aeromonas spp. and yersinia enterocolitica on cod and trout stored in two modified atmospheres at 0 degrees, 5 degrees and 12 degrees c was compared with that on aerobically stored fish. both organisms grew on aerobically stored fish at all three temperatures. growth on the modified-atmosphere-stored fish was never greater and was generally markedly less than on aerobically stored fish. this reduction in growth was greater in the higher carbon dioxide-containing atmosphere ...19958554761
tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria.a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ...19958554762
[soft-tissue infection caused by aeromonas hydrophila].two cases of soft tissue infection by aeromonas hydrophila are presented with review of the literature.19958555306
adhesion of aeromonas sp. to cell lines used as models for intestinal adhesion.adhesion to hep-2 cells has been shown to correlate with enteropathogenicity for aeromonas species. such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although hep-2 cells are not of intestinal origin. in this study strains of aeromonas veronii biotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to hep-2 cells and to an intestinal cell line (caco-2). quantitative assays showed identical a ...19958557078
purification and characterization of a cho cell-elongating toxin produced by aeromonas hydrophila.a chinese hamster ovary (cho) cell-elongating toxin produced by aeromonas hydrophila was purified from cell-free supernatant fluids by ammonium sulfate precipitation and fast protein liquid chromatography. the purified toxin had an isolelectric point (pl) of 3.7 and a molecular weight of 70,000 in a single band on isoelectric focusing (ief) gels and sds-page gels, respectively. the n-terminal sequence, amino acids 1-20, and the amino acid content were determined from western blots of the 70 kda ...19958559035
binding specificity for four monoclonal antibodies recognizing terminal gal alpha 1-->4gal residues in haemophilus influenzae lipopolysaccharide.four murine monoclonal antibodies (mabs) reactive with the outer-core region of the lipopolysaccharide (lps) from haemophilus influenzae were generated after immunization with azide-killed h. influenzae rm.7004 ah1-2 and their epitope specificities studied. the monoclonal antibodies: mahi 6 (igm), mahi 5 (igg2a), mahi 8 (igg3), and mahi 11 (igg2b) bound to synthetic glycoconjugates or glycolipids with terminal galabiosyl (gal alpha 1-->4gal beta 1-) or globotriaosyl (gal alpha 1-->4gal beta 1 1- ...19958559043
structural organization of a bacillus subtilis operon encoding menaquinone biosynthetic enzymes.menaquinone (mk) is a non-protein component of the bacillus subtilis (bs) electron transport chain synthesized from chorismate through a series of mk-specific reactions. the genes encoding biosynthesis of the naphthoquinone ring of mk are clustered at 273 degrees on the bs chromosome. a 3.9-kb region capable of rescuing men mutants blocked in the early stages of mk biosynthesis was sequenced and found to contain three major open reading frames (orfs). the first orf (menf) has a predicted size of ...19958566759
relationship between enterotoxicity and multiple drug resistance in aeromonas spp.one hundred forty-seven isolates of aeromonas spp., including 54 from diarrhoeal patients and 93 from the environmental sources, were examined for drug sensitivity and enterotoxin production. one hundred and fifteen (78%) isolates that included all a. hydrophila, 60% of a. sobria and 89% of a. caviae showed resistance to one or more antibiotic(s). most (65%) of the resistant isolates caused fluid accumulation in rabbit ileal loops, of these 57% were a. hydrophila, 69% of a. sobria and 65% a. cav ...19958568193
abundance of aeromonas spp. in river and lake waters in and around dhaka, bangladesh.aeromonas spp. are widely distributed in the aquatic environment. in bangladesh, seasonal distribution of these organisms in aquatic environment was studied during july 1986-august 1987. water, sediment, plant, and plankton samples were collected monthly from the dhanmondi lake and the buriganga river during the study period. aeromonas spp. were isolated from all samples throughout the year. the seasonal peak of aeromonas spp. was observed in warmer months (april-may) in the surface waters. in t ...19958568196
studies on bacteriocinogenic lactobacillus isolates from selected nigerian fermented foods.ten bacteriocin-producing (bacteriocinogenic) lactobacillus isolates obtained from three nigerian fermented foods namely: kenkey, ogi and wara were tested against the following indicator organisms: lactobacillus plantarum and food borne pathogens comprising enterotoxigenic escherichia coli, enterohaemorrhagic escherichia coli, serratia, pseudomonas, vibrio cholerae, aeromonas sobria, aeromonas cavice, salmonella typhimurium, plesiomonas shigelloides and staphylococcus aureus. all the bacteriocin ...19958568643
class d and e tetracycline resistance determinants in gram-negative bacteria from catfish ponds.dna probes were used to examine tetracycline-resistant gram-negative bacteria (281 strains representing eight species) from catfish ponds. the isolates, which did not previously hybridize with the tet a, b and c determinants, were examined for the presence of tetracycline-resistance tet d and tet e determinants. the distribution of the tet d determinant ranged from 0 to 83%, depending on the bacterial species. tet e was found only in 69% of aeromonas hydrophila isolates. eighteen per cent failed ...19958569770
improved glutaminate-starch-penicillin agar for the isolation and enumeration of aeromonas hydrophila from seawater by membrane filtration.glutaminate-starch-penicillin agar (gsp) in its original formula was found unsatisfactory for the detection and the enumeration of aeromonas hydrophila from seawater by membrane filtration. it was also the case when gsp agar was adjusted to ph 8 or when ampicillin (10 mg/l) was incorporated to the original formula. the elevation of ph to 8 together with the addition of 10 mg/l ampicillin gave to the medium a high selectivity and permitted the detection of a. hydrophila from seawater. the numbers ...19958570268
isolation, and virulence profiles, of aeromonas hydrophila implicated in an outbreak of food poisoning in sweden.a case of food poisoning outbreak involving aeromonas hydrophila is reported in this study. a group of 27 people consumed a typical swedish food "landgång" which is a type of smörgåsbord containing shrimps with mayonnaise, liver paté, ham, sausage, and legume salad which was purchased from a food store. twenty-two of the 27 persons became ill within 20-34 hr of consumption of the food and reported the symptoms ranging from severe acute diarrhea, abdominal pain, headache, fever and vomiting. one ...19958577278
an alternative bacteriological medium for the isolation of aeromonas spp.two solid bacteriologic media were compared for cultivating aeromonas spp. from piscine sources: the rimler-shotts (rs) medium and a starch-glutamate-ampicillin-penicillin-based medium (sgap-10c) used for the recovery of aeromonas spp. from water samples. the selective and differential capacities of the media were assessed march through october 1992 by recovery rate and phenotype of 99 isolates representing 15 genera of bacteria. recovery frequency of aeromonas spp. (n = 62) was similar at 97% o ...19958583652
cloning and sequence analysis of vibrio parahaemolyticus ompk gene encoding a 26-kda outer membrane protein, ompk, that serves as receptor for a broad-host-range vibriophage, kvp40.the ompk gene of vibrio parahaemolyticus 1010 (rimd 2210001) encoding an outer membrane protein (omp), ompk, which serves as the receptor for a broad-host-range vibriophage, kvp40, was cloned and sequenced. the gene consisted of 789 nucleotides encoding 263 amino acids. since the first 20 amino acids most likely constitute the signal peptide, mature ompk would consist of 243 amino acids with a calculated molecular mass of 27458 da. sequence comparisons indicate that ompk is unique among vibrio o ...19958586275
characterization of aeromonas trota strains that cross-react with vibrio cholerae o139 bengal.it has previously been shown that vibrio cholerae o139 bengal shares antigens with v. cholerae serogroups o22 and o155. we detected six surface water isolates of aeromonas trota that agglutinated in polyclonal antisera to v. cholerae o139 and v. cholerae o22 but not in antiserum to v. cholerae o155. on the basis of agglutinin-absorption studies, the antigenic relationship between the cross-reacting bacteria were found to be in an a,b-a,c fashion, where a is the common antigenic epitope and b and ...19958586685
study of the humoral response of atlantic salmon (salmo salar l.), naturally infected with aeromonas salmonicida ssp. achromogenes.the humoral antibody response of healthy atlantic salmon and of two groups of salmon, naturally infected with aeromonas salmonicida ssp. achromogenes, was examined in some detail. one diseased group was chronically infected and the other recently infected. it was found that the humoral response of these two infected groups was quite different. the chronically infected fish showed poor specific response to the causative agent whereas the recently infected salmon produced strong specific antibody ...19958588335
chronic diarrhea among adults in kigali, rwanda: association with bacterial enteropathogens, rectocolonic inflammation, and human immunodeficiency virus infection.one hundred patients with chronic diarrhea were seen in the department of internal medicine at the centre hospitalier de kigali, rwanda; stool and/or rectal swab culture was performed for these patients, and they underwent rectoscopy and serological testing for human immunodeficiency virus type 1 (hiv-1). enteropathogenic bacteria were isolated from 39 (39%) of the patients: shigella species (22 of 100 patients tested), non-typhi salmonella (11/100), aeromonas species (5/60), and campylobacter s ...19958589155
attachment of non-culturable toxigenic vibrio cholerae o1 and non-o1 and aeromonas spp. to the aquatic arthropod gerris spinolae and plants in the river ganga, varanasi.non-cultivable, pathogenic o1 and non-o1 vibrio cholerae and aeromonas spp. were resuscitated from aquatic arthropods and plant homogenate respectively, by rabbit ileal loop (ril) assay. these organisms adhered to the aquatic arthropod gerris spinolae and various species of phytoplankton in the river ganga, but failed to grow after direct inoculation on artificial media except for only 10 homogenates of the arthropod. the number of non-o1 v. cholerae and aeromonas recovered on direct inoculation ...19958589660
capsulated cells of aeromonas salmonicida grown in vitro have different functional properties than capsulated cells grown in vivo.when grown in vivo in the peritoneal cavity of rainbow trout, aeromonas salmonicida produces a clearly defined capsule with virulence-related functions. aeromonas salmonicida grown in vitro in a glucose-rich medium (grm) has also been reported to reproduce capsular material. because in vitro mimicry of in vivo induced traits is highly desirable in vaccine design, the extent to which growth in grm mimicked in vivo growth was examined. antibodies specific to in vivo grown cells partially labeled t ...19958590408
aeromonas encheleia sp. nov., isolated from european eels.four strains isolated from european eels in valencia, spain, were found to constitute a dna relatedness group which is 0 to 50% related to the 13 species and dna group 11 of the genus aeromonas. phenotypically, these strains have all of the properties that define the genus aeromonas. however, they differ from the previously described aeromonas species by three or more properties. the strains are positive for motility, growth at 37 degrees c, indole production, and arginine dihydrolase activity. ...19958590673
aeromonas hydrophila--its implications in freshwater injuries.lacerations or puncture wounds sustained in freshwater environments are susceptible to contamination by aeromonas hydrophila. numerous cases have been reported of cellulitis secondary to water-related injuries requiring hospitalization where a. hydrophila was the isolated organism. the typical presentation of an infection of a. hydrophila mimics a streptococcal soft tissue infection, which may result in delay in administration of appropriate antibiotics. a case is presented of a nonimmunocomprom ...19958590878
vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation.proaerolysin is an extracellular dimeric protein that is secreted across the inner and outer membranes of aeromonas spp. in separate steps. to investigate the role of protein folding in the second step, one or more cysteine residues were introduced and the mutant proaerolysins were expressed in aeromonas hydrophila and aeromonas salmonicida, as well as vibrio cholerae. replacing met-41 with cys resulted in expression of a protein that could form a dimer in which the monomers were linked together ...19958594324
cloning of a cluster of chitinase genes from aeromonas sp. no. 10s-24.a gene encoding chitinases from aeromonas sp. no. 10s-24 was cloned into escherichia coli dh5 alpha using puc19, and its nucleotides were sequenced. the chitinase gene was clustered in orfs (open reading frame) 1 to 4, in a 8-kb fragment of dna. orf-1 consisted of 1608 bp encoding 535 amino acid residues, and orf-2 consisted of 1425 bp encoding 474 amino acid residues. orf-3 was 1617 bp long and encodes a protein consisting of 538 amino acids. orf-4 encodes 287 amino acids of the n-terminal regi ...19968605248
identification of the catalytic triad of the lipase/acyltransferase from aeromonas hydrophila.aeromonas hydrophila secretes a lipolytic enzyme that has several properties in common with the mammalian enzyme lecithin-cholesterol acyltransferase. we have recently shown that it is a member of a newly described group of proteins that contain five similar blocks of sequence arranged in the same order in their primary structures (c. upton and j. t. buckley, trends biochem. sci. 233:178-179, 1995). assuming that, like other lipases, these enzymes have a ser-asp-his catalytic triad, we used thes ...19968606184
[aeromonas spp. as an indicator of the trophic state of bodies of water]. 19948611267
scanning electron microscopy analysis of bacterial colonization in crustaceans. 19948611268
characterisation of the heptameric pore-forming complex of the aeromonas toxin aerolysin using maldi-tof mass spectrometry.aerolysin, a virulence factor secreted by aeromonas hydrophila, is representative of a group of beta-sheet toxins that must form stable homooligomers in order to be able to insert into biological membranes and generate channels. electron microscopy and image analysis of two-dimensional membrane crystals had previously revealed a structure with 7-fold symmetry suggesting that aerolysin forms heptameric oligomers [wilmsen et al. (1992) embo j. 11, 2457-2463]. however, this unusual molecularity of ...19968617369
aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, ompa.two homologs of the outer membrane protein ompa were identified in aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. an a. salmonicida genomic dna library was constructed by using lambda gem-11 and recombinant phage carrying both genes ompai and ompaii) selected by immunoscreening. a 5.0-kb bamhi fragment containing the two genes in tandem was subcloned in pbluescript and used for further subcloning and seque ...19968626290
fatal aeromonas hydrophila bacteremia in a hemodialysis patient treated with deferoxamine.a 49-year-old woman undergoing long-term hemodialysis and treated with deferoxamine (dfo) 1.5 g twice weekly for aluminum bone disease developed fever and bilateral calf pain caused by myonecrosis with gas gangrene. she had a rapidly fatal outcome. the cultures of blood and aspirates from both calf muscles demonstrated aeromonas hydrophila. no obvious entry point could be traced. the in vitro growth of the patient's strain was found to be stimulated by the deferoxamine-iron complex in an iron-de ...19968629637
biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental aeromonas isolates.one hundred and eight strains of aeromonas from clinical and environmental samples were speciated. seven species were identified, the most prevalent of which was a. hydrophila. experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant fluid accumulation in rabbit ileal loops. of 107 strains showing single or multiple antimicrobial resistance, the highest incidence of resistance was shown for beta-lactam antibioti ...19968636960
behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacilli.in this study, behaviour of yersinia enterocolitica and aeromonas hydrophila in skim milk during fermentation by various lactobacillus sp. were determined. ph values of the skim milk samples were also examined during fermentation. the amount of produced lactic acid and diacetyl/acetoin productions of the lactobacillus sp. were estimated. antimicrobial effects of the lactobacilli on y. enterocolitica and a. hydrophila were also determined by an agar diffusion method. while y. enterocolitica was n ...19968638435
[clinical and laboratory significance of inducible beta-lactamases].many species of bacteria have inducible expression of beta-lactamase and the enzyme production in these bacteria is normally held at a low level by a repressor mechanism, but in the presence of a beta-lactamase inducer this repression is lifted and enzyme production is greatly increased. inducible synthesis of beta-lactamase was first described for the gram-positive organism bacillus licheniformis. after that inducible expression of beta-lactamase was discovered in gram-negative bacteria like en ...19958643019
growth and exotoxin production at low temperatures by aeromonas strains in meat slurries.the ability of enterotoxigenic strains of aeromonas hydrophila and aeromonas sobria to produce exotoxins (haemolysin and enterotoxin) and exoenzymes (protease and lipase), as well as their growth in meat slurries stored at low temperatures (5 and 12 degrees c) was investigated. the results showed that aeromonas grew well in meat slurries stored at low temperatures in the presence of background flora. exotoxins and exoenzymes were not detected in filtrates from meat slurries inoculated with enter ...19968643030
the structure of the aeromonas proteolytica aminopeptidase complexed with a hydroxamate inhibitor. involvement in catalysis of glu151 and two zinc ions of the co-catalytic unit.the structure of the complex of aeromonas proteolytica aminopeptidase, a two-zinc exopeptidase, with the inhibitor p-iodo-d-phenylalanine hydroxamate has been determined by x-ray crystallography. refinement of the structure, which includes 220 water molecules, using data at 0.80-0.23-nm resolution resulted in a crystallographic residual r value of 16%. the hydroxamate group adopts a planar conformation whereby the two oxygen atoms interact with the zinc ions. the n-hydroxyl group of the inhibito ...19968647077
the 'hidden' carbapenemase of aeromonas hydrophila.it has been presumed that there are just two beta-lactamases in the motile aeromonas species, a carbapenemase and a cephalosporinase, based on the premise that all beta-lactamases can be detected by hydrolysis of the chromogenic cephalosporin, nitrocefin. however, when it was recently found that a non-motile species of aeromonas that causes furunculosis in salmon, contained three beta-lactamases, one of which was a carbapenemase which could not be detected with nitrocefin, it was hypothesised th ...19968647771
survival and growth of aeromonas hydrophila on modified atmosphere packaged normal and high ph lamb.the growth of a. hydrophila on normal ph (5.5-5.8) and high ph ( > 6.0) lamb stored under modified atmospheres was examined. lamb pieces and mince, inoculated with a. hydrophila were packaged in air, vacuum pack, 80% o2/20% co2, 50% co2/50% n2, or 100% co2 and stored at 5 or 0 degrees c for up to 42 days. samples were examined for the survival and/or growth of a. hydrophila by enrichment in alkaline peptone water and/or direct plating on starch ampicillin agar. the ph of each sample was estimate ...19968652346
gastroenteritis caused by aeromonas trota in a child.a case of acute diarrhoea caused by aeromonas trota (formerly hg 13 group) in a spanish child is reported. the strain was isolated in the faeces using the cin agar (cefsulodin-irgasan-novobiocin) culture media. the strain was initially identified as a sobria by the commercial gni card and api 20e biochemical systems. the strain was, however, vogesproskauer and sucrose negative, so complementary tests of cellobiose fermentation and gluconate oxidation were performed. these tests, together with th ...19968655689
characterization of aeromonas hydrophila strains of clinical, animal, and environmental origin expressing the o:34 antigen.a collection of aeromonas strains of different origins were characterized for isolates expressing the o:34 somatic antigen. of over 200 strains tested, approximately 14% belonged to serogroup o:34 with >85% of these strains identified as a. hydrophila regardless of source. a subset of 14 a. hydrophila o:34 strains were further analyzed for a number of structural and pathogenic features. most o:34 strains expressed similar whole-cell protein profiles with regards to minor bands, but major band di ...19968662181
aminopeptidase from streptomyces griseus: primary structure and comparison with other zinc-containing aminopeptidases.the aminopeptidase from streptomyces griseus is a calcium-activated metalloenzyme, which contains 2 mol tightly bound zinc/mol protein. this aminopeptidase rapidly hydrolyzes peptide bonds formed by n-terminal hydrophobic amino acids, such as leucine, methionine and phenylalanine. we have determined the complete primary structure of the protein, which contains 284 amino acid residues, yielding a molecular mass of 29723 da. a search in the swiss-prot database for sequence similarities revealed a ...19968665903
structure of the o-specific polysaccharide of an aeromonas trota strain cross-reactive with vibrio cholerae o139 bengal.the o-specific polysaccharide of an aeromonas trota strain was isolated by hydrolysis of the lipopolysaccharide at ph 4.5 followed by gel-permeation chromatography and found to consist of hexasaccharide repeating units containing d-galactose, l-rhamnose, 3,6-dideoxy-l-xylo-hexose (colitose, col), 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galactose in the ratios 1:1:2:1:1. partial hydrolysis of the polysaccharide with 48% hydrofluoric acid resulted in selective removal of colitose t ...19968665933
the o:34-antigen lipopolysaccharide as an adhesin in aeromonas hydrophila.we compared the ability of different aeromonas hydrophila strains from serogroup o:34 grown at different temperatures to adhere to hep-2 cells. we found a high level of adhesion when the strains were grown at 20 degrees c but not when they were grown at 37 degrees c. we previously described that these strains were able to form the o-antigen lipopolysaccharide when they grow at low temperature but not at high temperature. we also obtained by transposon mutagenesis mutants only devoid of the o-ant ...19968674995
endotoxin in aeromonas sobria infection: speculative pathogenesis and treatment strategies in advanced liver disease with soft-tissue infection. 19968678032
infections in the cirrhotic patient. 19968678033
[bacteremic peritonitis caused by aeromonas hydrophila in a woman with cirrhosis]. 19968679853
cloning, sequencing, and characterization of the nuch gene encoding an extracellular nuclease from aeromonas hydrophila jmp636.an escherichia coli clone expressing activity on dnase agar was obtained by cloning chromosomal dna of aeromonas hydrophila jmp636 into plasmid puc19. examination (of the clone's nuclease activity on a sodium dodecyl sulfate (sds)-polyacrylamide gel containing dna as a substrate revealed an activity band at approximately 100 kda. subsequently, subcloning localized the gene, designated nuch, to a 3.6-kb dna fragment (pjp9521). southern blotting of the nuch gene against chromosomal dna of jmp636 c ...19968682799
a multiplicity of bowel problems.a 55-year-old man presented with a 12-pound weight loss and acute diarrhea. he had had three watery stools a day for 12 days, as well as fever, chills, abdominal pain, nausea, and vomiting. he had not had any bowel movements at night, but noted that the first one each morning was tinged with blood.19968682885
immunotoxic effects of copper and cadmium in the sea bass dicentrarchus labrax.two phagocytes-mediated activities of the sea bass dicentrarchus labrax were examined after exposure to sublethal concentrations of copper and cadmium: (a) phagocytosis (measured by phagocytotic index), and (b) the production of reactive oxygen intermediates (luminol-dependent chemiluminescence) in response to bacteria aeromonas salmonicida. in vivo exposure for 48 h to each metal separately by intraperitoneal injection did not affect the quantity of phagocytes of pronephros and their viability ...19968683034
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probesthe structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ...19968688004
characterization of atypical aeromonas salmonicida isolates by ribotyping and plasmid profiling.a total of 38 strains of atypical aeromonas salmonicida, three oxidase-negative but otherwise typical aer. salmonicida, three typical aer. salmonicida, and two reference strains, isolated from several countries and fish species were examined with respect to rrna gene restriction patterns (ribotypes) and plasmid profiles. most epidemiologically unrelated strains had different ribotypes, whereas isolates from the same outbreak were identical. all strains, except one, carried one or more large plas ...19968698652
bacteria in surface infections of neonates.a bacteriological work on surface infections was done among live births (study group i) and neonates admitted in hospital (study group ii). out of 134 cases of conjunctivitis in group i gram-negative bacilli predominated (48.5%) with escherichia coli accounting for 29 (14.9%) cases, klebsiella species 15 (11.2%) cases, citrobacter freundii 3 (2.2%) cases, pseudomons aeruginosa 18 (13.4%) cases and aeromonas hydrophila 3 (2.2%) amongst pure isolates (73.9%). gonococcus was noted in 2 (1.5%) cases ...19958699037
bacterial aminopeptidases: properties and functions.aminopeptidases are exopeptidases that selectively release n-terminal amino acid residues from polypeptides and proteins. bacteria display several aminopeptidasic activities which may be localised in the cytoplasm, on membranes, associated with the cell envelope or secreted into the extracellular media. studies on the bacterial aminopeptide system have been carried out over the past three decades and are significant in fundamental and biotechnological domains. at present, about one hundred bacte ...19968703509
utilization of transferrin and salmon serum as sources of iron by typical and atypical strains of aeromonas salmonicida.the ability of typical and atypical strains of aeromonas salmonicida to utilize non-haem sources of protein-bound iron was evaluated. (i) in a plate bioassay, the suppression of growth imposed on typical and atypical a. salmonicida by addition of the high-affinity iron chelator ethylenediamine-di(o- hydroxyphenylacetic acid) (edda) to the growth medium was reversed by the addition of 30% or 90% iron-saturated bovine or human transferrin (tf) or lactoferrin (lf) to the growth medium. (ii) the mec ...19968704995
microbiological quality & incidence of organisms of public health importance in food & water in ludhiana.bacteriological analysis of 713 samples of various types of foods and related articles and potable water samples from different places in ludhiana, punjab was carried out. the highest counts ranging from 2.5 x 10(6)-7.5 x 10(8) organisms/g were observed in raw vegetables and fruits, followed by 3 x 10(6)-9.8 x 10(7)/ml, 8.3 x 10(4)-8.9 x 10(7)/g and 1 x 10(3)-6.7 x 10(7)/g in fruit juice, milk and its products, and salty/non milk snacks respectively. fresh chapati, dal, rice, cooked vegetables a ...19968707360
colonization of streptomycin-treated mice by aeromonas species.streptomycin-treated adult mice were investigated as a possible model for studying the enteropathogenicity of aeromonas species. c57bl mice pre-treated with streptomycin (5.0 g/l drinking water, 48 hours) received a single intragastric dose (10(10) bacteria /10.5 ml) of one of six well-characterized, toxin-producing, human diarrhoeal isolates of a. veronii biovar sobria (n = 3) or a. hydrophila (n = 3). their faeces were examined for aeromonas for 10 days post-challenge. all strains colonized th ...19968708329
combined effect of gamma-irradiation and conventional cooking on aeromonas hydrophila in meatball.irradiation combined with a conventional cooking procedure was applied to meatball and the effects on bacterial load and inoculated aeromonas hydrophila were determined. meatball samples were irradiated by using a 60co source at the dose levels of 0, 0.30, 0.75, 1.50, 2.50 kgy and cold stored at 4 +/- 1 degrees c for 7 days. bacterial load and the count of a. hydrophila decreased when the irradiation dose level increased. a minimum inhibition effect was found at the dose of 0.30 kgy. irradiation ...19968717095
aeromonas bacteraemia in burn patients.human aeromonas infection is uncommon and is usually associated with immunosuppression, chronic disease or trauma in an aquatic setting. burn injury may induce a state of immunosuppression, making the thermally injured patient a suitable host for aeromonas infection. we reviewed the experience of one burn centre with this pathogen. retrospective examination of blood culture results from 8151 patients admitted between 1959 and 1994 disclosed eight patients with clinically relevant aeromonas hydro ...19968719317
successful treatment of aeromonas hydrophila peritoneal dialysis-associated peritonitis using intermittent intraperitoneal gentamicin. 19958719554
using a new inbred fish model and cultured fish tissue cells to study aeromonas hydrophila and yersinia ruckeri pathogenesis.an inbred strain of the southern platyfish, xiphophorus maculatus, was used as a host for aeromonas hydrophila and yersinia ruckeri infections. the infections were initiated by holding the platyfish in inoculation baths containing dilutions of virulent a. hydrophila or y. ruckeri strains. inoculating the platyfish in this manner resulted in a dose-dependent mortality over a range of bacterial input from 10(5) to 10(8) a. hydrophila and 10(6) to 10(8) y. ruckeri/ml. clinical manifestations of a. ...19968722100
prevalence and serovar distribution of salmonella in fresh and frozen meat from captive nile crocodiles (crocodylus niloticus).salmonella was isolated from 6 of 20 (30.0%) samples of fresh meat, and from 28 of 140 (20.0%) samples of frozen meat processed for human consumption from captive nile crocodiles (crocodylus niloticus) in zimbabwe. salmonella enterica isolates showed a serovar distribution of 41.2% (14/34) subsp. enterica, 11.8% (4/34) subsp. salamae and 41.2% (14/34) subsp. diarizonae. analyses of fresh meat samples yielded aeromonas (a. hydrophila) in 18 of 20 samples (90%), and a mean aerobic plate count (apc ...19968722192
development of aeromonas hydrophila bacteremia in a patient recovering from cholera. 19968722957
cloning and nucleotide sequence of the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida.the dna gyrase gyra gene from the fish pathogen aeromonas salmonicida 2148/89 was cloned, and the nucleotide sequence was determined. an open reading frame of 2,766 nucleotides was identified and was found to encode a protein of 922 amino acids with a calculated molecular mass of 101.1 kda. the derived amino acid sequence shared a high degree of identity with other dna gyrase a proteins, in particular, with other gram-negative gyra sequences. when the amino acid sequence of a. salmonicida gyra w ...19968723452
increasing antibiotic resistance in clinical isolates of aeromonas strains in taiwan.a total of 234 clinical isolates of aeromonas, primarily a. hydrophila, were collected for the present study. most were isolates from blood. by the agar dilution method, more than 90% of the aeromonas strains were found to be susceptible to moxalactam, ceftazidime, cefepime, aztreonam, imipenem, amikacin, and fluoroquinolones, but they were more resistant to tetracycline, trimethoprim-sulfamethoxazole, some extended-spectrum cephalosporins, and aminoglycosides than strains from the united states ...19968723478
exploring the use of the medicinal leech: a clinical risk-benefit analysis.the medicinal leech, hirudo medicinalis, has been used with increasing frequency for salvage of compromised pedicled flaps and microvascular free-tissue transfers. the data pertaining to 18 cases in which flap salvage with leeches was attempted were reviewed and contrasted with the data from 108 published cases from other centers. a further analysis isolated and examined 19 cases in which infection, secondary to leech utilization, supervened. results suggested that successful salvage of tissue w ...19968726336
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