Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum. | eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ... | 2001 | 11181369 |
| enteropathogenicity of plesiomonas shigelloides and aeromonas spp. in experimental mono- and coinfection with cryptosporidium parvum in the intestine of neonatal balb/c mice. | enteropathogenicity of plesiomonas shigelloides, aeromonas hydrophila, a. caviae and a. sobria was studied both in monoinfections and in coinfections with coccidium cryptosporidium parvum in neonatal balb/c mice. in monoinfection experiments, neonatal balb/c mice were orally infected with 7 x 10(7) or 7 x 10(8) cfu, respectively, of a strain of p. shigelloides or a strain of an aeromonas spp. in coinfection experiments, the neonatal mice were, in addition to being orally infected with one of the ... | 2001 | 11131040 |
| crystallization and preliminary x-ray analysis of (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis. | dimeric (r)-specific enoyl-coenzyme a (coa) hydratase from aeromonas caviae catalyzes the hydration of trans-2-enoyl-coas with carbon lengths of 4-6 to yield their corresponding (r)-3-hydroxyacyl-coas and is essential for polyhydroxyalkanoate (pha) biosynthesis. the enzyme has been crystallized by vapour diffusion against a reservoir solution containing 20% polyethylene glycol 4000, 5% 2-propanol and 20 mm hepes ph 7.0 at 298 k. crystals belong to the monoclinic space group c2, with unit-cell pa ... | 2001 | 11134939 |
| in vitro and in vivo combinations of cefotaxime and minocycline against aeromonas hydrophila. | the activities of cefotaxime and minocycline against aeromonas hydrophila were investigated. cefotaxime (4 times the mic) plus minocycline (0.75 times the mic) elicited an inhibitory effect for 48 h in a time-kill study, and more infected mice treated with both drugs survived (91%) than survived after treatment with cefotaxime (9%) or minocycline (44%) alone, suggesting that cefotaxime and minocycline act synergistically against a. hydrophila. | 2001 | 11257047 |
| identification of a 43-kda outer-membrane protein as an adhesin in aeromonas caviae. | aeromonas spp. are associated with intestinal and extra-intestinal infections. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interactions involved in the adherence of a. caviae isolates ae56, ae391 and ae398 to hep-2 cells. all strains expressed high levels of aggregative adherence. maximum adhesion occurred with bacteria grown at 22 degrees c, but transmission electron microscopy did not reveal the presence of fimbrial structures on ... | 2001 | 11289516 |
| locomotion and feeding of acanthamoeba at the water-air interface of ponds. | acanthamoeba trophozoites attach to and effect amoeboid locomotion at the water-air interface of ponds. their locomotory rate (approximately 0.8 microm s(-1)) and manner of independent movement at this interface is similar to that over solid substrata. adhesion forces developed between amoebae and the water-air interface are greater than gravity and thus amoebae are also transported passively without detachment. amoebae docked with the water-air interface remain and flourish here as they are sho ... | 2001 | 11164298 |
| role of flm locus in mesophilic aeromonas species adherence. | the adherence mechanism of aeromonas caviae sch3n to hep-2 cells was initially investigated through four mini-tn5 mutants that showed a 10-fold decrease in adherence. these mutants lost motility, flagella, and their lipopolysaccharide (lps) o antigen (o-ag). three genes, flmb-neua-flmd, were found to be interrupted by the transposon insertions; additionally, two other genes, one lying upstream (flma) and one downstream (neub), were found to be clustered in the same operon. while the flma and flm ... | 2001 | 11119490 |
| [metallo-beta-lactamase producing bacteria]. | metallo-beta-lactamases are the carbapenemases belonging to the class b beta-lactamases on a molecular level. two types of the metallo-beta-lactamase, imp-1 and vim-1, which have been detected mainly from pseudomonas aeruginosa, respectively, in japan and in europe are especially important, because of the broad substrate specificity. these enzymes confer resistance to not only the carbapenems but also almost all beta-lactam antibiotics expect for monobactams. clinical strains producing imp-1 typ ... | 2001 | 11304992 |
| the occurrence of aeromonas spp. in natural mineral water and well water. | the presence of motile aeromonas spp. in natural mineral water (nmw) and drinking well water in the area of lecce (italy) was investigated. aeromonas spp. were not detected in any of the 60 nmw samples either by the direct and enrichment method. from a total of 20 wells, five were found to contain aeromonas species with cell number ranging from 26 to 1609 250 ml(-1). in two wells the presence of aeromonas spp. was not associated to the presence of faecal indicators, i.e. coliforms and faecal col ... | 2001 | 11205949 |
| characterisation of the culturable heterotrophic bacterial community in a small eutrophic lake (priest pot). | the community composition and structure of planktonic heterotrophic bacteria (903 isolates) sampled from a small eutrophic lake in northern england (priest pot) was studied with respect to season (four samples) and depth (to 3.1 m). bacteria (887) were isolated on tryptic soy broth agar and identified to 48 genera using fatty acid methyl ester analysis. the two most abundant genera isolated were aeromonas and pseudomonas which, respectively, dominated the middle to bottom depths in august and al ... | 2001 | 11311440 |
| antibacterial effects of different food-related phosphates using aeromonas hydrophila. | aeromonas hydrophila is considered to be an emergent food-related bacterium. phosphates are used as additives, mainly in meat products, to improve the quality of these foods. the antibacterial properties of phosphates are also well known. in this work, two a. hydrophila strains in early exponential phase were used: (a) a. hydrophila atcc 7965 and (b) a. hydrophila derived from food, isolated in our laboratory. mic and mbc studies were performed to assess the antibacterial effects of four phospha ... | 2001 | 11271767 |
| biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates. | one hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. analysis of biodiversity at the species level was performed by comparing the alui restriction patterns of the 16s ribosomal dna amplified by pcr from 120 isolates. the rdna restriction analysis enabled us to c ... | 2001 | 11281319 |
| the occurrence of aerolysin-positive aeromonas spp. and their cytotoxicity in norwegian water sources. | the aim of the study was to investigate the occurrence of aeromonas spp. and their aerolysin status in norwegian natural water sources. | 2001 | 11348441 |
| prevalence, species differentiation, haemolytic activity, and antibiotic susceptibility of aeromonads in untreated well water. | the use of untreated water for drinking and other activities have been associated with intestinal and extraintestinal infections in humans due to aeromonas species. in the present study aeromonads were isolated from 48.7% of 1,000 water samples obtained from wells and other miscellaneous sources. aeromonas species were detected in 45% of samples tested in spring, 34.5% in summer, 48% in autumn and 60% of samples tested in winter. speciation of 382 strains resulted in 225 (59%) being a. hydrophil ... | 2001 | 11285492 |
| enhancement of the virulence of aeromonas caviae diarrhoeal strains by serial passages in mice. | thirteen clinical aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (ctc) factor production, by four consecutive passages through mice by intraperitoneal injection of a. caviae suspensions. the passaged a. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. all a. caviae isolates pos ... | 2001 | 11289515 |
| centa as a chromogenic substrate for studying beta-lactamases. | centa, a chromogenic cephalosporin, is readily hydrolyzed by beta-lactamases of all classes except for the aeromonas hydrophila metalloenzyme. although it cannot practically be used for the detection of beta-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions. | 2001 | 11353639 |
| diarrhoeal infection with aeromonas species. | 2001 | 11291990 | |
| microbial growth modelling with artificial neural networks. | there is a growing interest in modelling microbial growth as an alternative to time-consuming, traditional, microbiological enumeration techniques. several statistical models have been reported to describe the growth of different microorganisms, but there are accuracy problems. an alternate technique 'artificial neural networks' (ann) for modelling microbial growth is explained and evaluated. published data were used to build separate general regression neural network (grnn) structures for model ... | 2001 | 11294356 |
| formation of viable but non-culturable state (vbnc) of aeromonas hydrophila and its virulence in goldfish, carassius auratus. | in this study we investigated the viable but non-culturable (vbnc) state of aeromonas hydrophila and its virulence in goldfish. aeromonas hydrophila cultured in a 0.35% nacl solution at ph 7.5 and at 25 degrees c for 50 days showed the vbnc state. in the vbnc state we were unable to detect viable bacteria by the plate count method but we did find 10(4) cells/ml by the direct viable count microscopical method after staining with fluorescein diacetate and ethidium bromide. the virulence comparison ... | 2001 | 11372646 |
| characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish. | the present study aimed to investigate the potential probiotic properties of six lactic acid bacteria (lab) intended for human use, lactobacillus rhamnosus atcc 53103, lactobacillus casei shirota, lactobacillus bulgaricus, l. rhamnosus lc 705, bifidobacterium lactis bb12, and lactobacillus johnsonii la1, and one for animal use, enterococcus faecium tehobak, for use as a fish probiotic. the strains for human use were specifically chosen since they are known to be safe for human use, which is of m ... | 2001 | 11375147 |
| 16s rrna in situ probing for the determination of the family level community structure implicated in enhanced biological nutrient removal. | knowledge of a discrete physiological group capable of excess biological phosphate removal (ebpr) remains unclear. consequently, microbial community analysis of an enhanced continuous laboratory-scale activated sludge process displaying a strong ebpr mechanism was conducted. unit design was configured upon the three-stage phoredox process and characterization of the activated sludge bacterial community was carried out using fluorescent in situ hybridization (fish) techniques. fixed activated slu ... | 2001 | 11379117 |
| association between major histocompatibility complex class iib alleles and resistance to aeromonas salmonicida in atlantic salmon. | we have tested the importance of genetic variation in the major histocompatibility complex (mhc) class iib in atlantic salmon (salmo salar) for survival after challenge with a highly virulent bacterial pathogen. forty juvenile full siblings from each of 120 families were infected with the bacterium aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. fishes from high-resistance (hr, < 35% mortality) and low-resistance (l,r, > 80% mortality) families were screened for ... | 2001 | 11296859 |
| characterization of class 1 integrons associated with r-plasmids in clinical aeromonas salmonicida isolates from various geographical areas. | class 1 integrons were found in 26 of 40 antibiotic-resistant isolates of the fish pathogen aeromonas salmonicida from northern europe and north america. three different dhfr genes, conferring trimethoprim resistance, and one ant(3")1a aminoglycoside resistance gene were identified as gene inserts. the gene cassettes tended to be conserved among isolates from a particular geographical area. nineteen isolates transferred r-plasmids carrying different tet determinants to escherichia coli in filter ... | 2001 | 11389105 |
| fulminant pneumonia due to aeromonas hydrophila in a man with chronic renal failure and liver cirrhosis. | a 40-year-old man on hemodialysis was admitted due to dyspnea and chest pain and was diagnosed with pneumonia and pericarditis. ampicillin was administered, but thereafter severe septic shock developed. the fulminant type of pneumonia progressed rapidly, and he died only 48 hours after the onset of symptoms. the autopsy and sputa culture revealed pneumonia due to aeromonas hydrophila. the source of this infection remained unkown. interestingly, there were two types of a. hydrophila found during ... | 2001 | 11300143 |
| characterization of the pro-aminopeptidase from aeromonas caviae t-64. | the pro-aminopeptidase from aeromonas caviae t-64 (pro-apac) had maximal activity at 60 degrees c and was more stable than mature apac at temperature up to 65 degrees c for 1 hour. the ph stability of pro-apac ranged from 4.0 to 8.0, which is broader than the range for the mature apac. the kcat/km of pro-apac was 1.4% to 24% of that of mature apac. | 2001 | 11302179 |
| synergy of histone-derived peptides of coho salmon with lysozyme and flounder pleurocidin. | recent research has identified endogenous cationic antimicrobial peptides as important factors in the innate immunity of many organisms, including fish. it is known that antimicrobial activity, as well as lysozyme activity, can be induced in coho salmon (oncorhynchus kisutch) mucus after exposure of the fish to infectious agents. since lysozyme alone does not have antimicrobial activity against vibrio anguillarum and aeromonas salmonicida, a four-step protein purification protocol was used to is ... | 2001 | 11302792 |
| comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in belgium. | the aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxytetracycline-resistant (ot(r)) heterotrophic bacterial population in two belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. the study of ot(r) heterotrophs and mesophilic aeromonas spp. allowed comparison of tolerance data at the intergenus as well as at the intragenus level. in total, 354 independently obtained ot(r) is ... | 2001 | 11403391 |
| occurrence and antibiotic resistance of mesophilic aeromonas in three riverine freshwaters of marrakech, morocco. | in order to evaluate the impact of pollution and sewage on the occurrence and antibiotic resistance of mesophilic aeromonads in riverine freshwaters of marrakech, samples were collected from three rivers (oukaimeden, ourika, and tensift) upstream and downstream from the principal bordering villages. during a 2-year study, indicators of pollution increased dramatically in the downstream waters. bacterial indicators (faecal coliforms and faecal streptococci) correlated with mesophilic aeromonads o ... | 2001 | 12805714 |
| an expression release system for heterogeneous proteins in e.coli mediated by the kil gene. | an expression release system for heterogeneous proteins in escherichia coli mediated by the colicin release gene(kil gene) was constructed. the system is based on the ability of the kil protein to release periplasmic proteins into the growth medium. beta-lactamase, an e.coli periplasmic protein, and prolyl endopeptidase (pep), a periplasmic protein of aeromonas punctata subsp. punctata were used as report proteins. commonly, these two proteins are seldom released into the growth medium. the resu ... | 2001 | 12040395 |
| role of bacteria in the epizootic ulcerative syndrome (eus) of fishes. | bacteriological examination of certain water bodies and fishes carrying eus was carried out. as a whole, 17 species of bacteria were isolated from the investigated water bodies and eus affected fishes. the species of bacteria isolated from fishes are common to those isolated from water. experimental infection trials conducted suggested that aeromonas hydrophila in association with pseudomonas fluorescens, may be playing the role of primary aetiological agent in producing eus in fishes. | 2001 | 12017259 |
| correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity. | certain strains of mesophilic aeromonads like a. hydrophila, a. veronii biotype sobria and a. caviae when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. as these strains become nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as means of understanding animal virulence and enteropathogenicity. non suicidal strains of a. hydrophila showed and overall 88.8% lethality rate and non ... | 2001 | 12035354 |
| direct detection of diarrheagenic aeromonas from faeces by polymerase chain reaction (pcr) targeting aerolysin toxin gene. | detection of diarrheagenic aeromonas specific aerolysin toxin (aer) gene by pcr based assay and isolation, identification of diarrhea causing aeromonas from faeces by culture methods were carried out in the division of active surveillance, national institute of cholera and enteric diseases (niced), kolkata, india for a period of 12 months. out of 602 faecal samples collected from patients with acute diarrhea admitted in infectious diseases (id) hospital, kolkata, 68 (11.29%) samples were found t ... | 2001 | 12004918 |
| [in vitro evaluation of antibacterial substances produced by bacteria isolated from different marine organisms]. | bacteria from several groups of marine organisms were isolated and, using direct antibiograms, identified those that produce antibacterial substances, using a human pathogenic strain of staphylococcus aureus atcc6538 as revealing microorganism. bacteria which produce substances that inhibited s. aureus growth were identified through morphological, physiological and biochemical tests. out of 290 bacteria, 54 (18.6%) inhibited the growth of s. aureus, but only 27 survived for identification. bival ... | 2001 | 12189804 |
| isolation of hexavalent chromium-reducing cr-tolerant facultative anaerobes from tannery effluent. | several facultative anaerobes tolerant to high levels of chromate (>400 mg/ml) were isolated from tannery effluents. these isolates displayed varying degrees of cr(vi) reduction under aerobic and anaerobic conditions at room temperature (24+/-2 degrees c). interestingly, eight isolates were efficient in reducing 70% cr(vi) anaerobically. this includes 5 isolates of genus aerococcus, two isolates of micrococcus and single isolate of genus aeromonas. these isolates were subjected to further charac ... | 2001 | 12483605 |
| childhood gastroenteritis due to aeromonas hydrophila in ibadan, nigeria. | this is a prospective study spanning a period of six months where stools of 400 diarrhoeic children and 100 non-diarrhoeic children aged 0-5 years were screened for aeromonas hydrophila and other enteric pathogens. out of the 400 diarrhoeic stools only three (0.75%) were positive for aeromonas hydrophila while none was positive in the control group. no other enteric pathogen was isolated from these positive samples, indicating that aeromonas hydrophila is responsible for their diarrhoea. continu ... | 2001 | 14510117 |
| isolation and identification of aeromonas from patients with acute diarrhoea in kolkata, india. | isolation of diarrhoea causing aeromonas was carried out in the division of active surveillance, national institute of cholera and enteric diseases (niced), kolkata for a period of 12 months from january 1999 to december 1999. out of 602 stool samples collected from patients with acute diarrhoea admitted in infectious diseases (id) hospital, kolkata, 64 (10.6%) samples were identified positive for aeromonas as the pathogen. the different isolated and identified species from patients with acute d ... | 2001 | 17664830 |
| expression of a gene encoding chitinase (pca 8 orf) from aeromonas sp. no. 10s-24 in escherichia coli and enzyme characterization. | a gene encoding chitinase from aeromonas sp. no. 10s-24 was expressed using ptrc99a in escherichia coli jm 105 which yielded a 5-fold higher activity than when puc19 was used. three different truncated enzymes (sa-1, sa-2 and sa-3) were obtained after purification. their isoelectric points were 7.0, 6.9, and 6.7, respectively. the enzymes showed two optimum phs, 4.0 and 7.0, when incubated with ethylene glycol chitin as the substrate, and were stable over a wide ph range (3.0-9.0). the optimum t ... | 2001 | 16233047 |
| cloning, nucleotide sequence and expression of the beta-n-acetylglucosaminidase gene from aeromonas sp. no. 10s-24. | the beta-glcnacase gene of aeromonas sp. no. 10s-24 was cloned and sequenced. the gene consisted of 2505 bp encoding 835 amino acid residues. the amino acid sequence of the gene shares sequence homology with beta-glcnacases of vibrio vulnificus (37.4%), serratia marcescens (36.4%), v. harveyi (36.4%), v. parahemolyticus (33.5%), alteromonas sp. strain o-7 (27.0%), and candida albicans (28.6%). the beta-glcnacase gene of aeromonas sp. no. 10s-24 was about 630-fold overexpressed in escherichia col ... | 2000 | 16232720 |
| isolation and characterization of aeromonas sp. b-5 capable of decolorizing various dyes. | aeromonas sp. b-5, which has the ability to decolorize azo dyes, was isolated from soil. aeromonas sp. b-5 completely decolorized 100 mg/l of bordeaux s by reductive cleavage of azo bonds under static conditions in 24 h. though the decolorization of bordeaux s by aeromonas sp. b-5 was suppressed under shaking, rapid decolorization was observed when the culture was changed to static conditions after cultivation with shaking. the indigoid dye, acid blue 74, was decolorized by aeromonas sp. b-5 und ... | 2000 | 16232913 |
| high-efficiency expression of prolyl endopeptidase from aeromonas punctata subsp. punctata in escherichia coli. | the open reading frame (orf) of prolyl endopeptidase gene from aeromonas punctata subsp. punctata (appep) was amplified by pcr in three parts. the amplified dna segments were ligated to form the complete appep gene, and then cloned into expression vectors pbl (temperature inducible) and pkkh (iptg inducible), respectively. after induction, the expression amounts of recombinant appep in bl21/pkkh-pep and bl21/pbl-pep were about 30% of the total bacterial proteins, and the enzyme activities were 1 ... | 2000 | 12098799 |
| [production, partial purification and characterization of methamidophos-degrading enzyme from methylotroph wb-1]. | after cultivation for 20 h in the inorganic salt medium with methamidophos as sole carbon and nitrogen source, methylotroph wb-1 could produce methamidophos-degrading enzyme in larger amounts. the enzyme was partially purified by sonication disruption, tween-20 extraction, heat treatment(9 min at 60 degrees c), deae-cellulose 32 and cm-cellulose32 chromatography with 22.8 times purification and 78.8% recovery. activity staining showed single violate band corresponding to that of protein staining ... | 2000 | 12548765 |
| [purification and identification of siderophore from aeromonas hydrophila]. | siderophore of aeromonas hydrophila(ah) j-1 was purified from culture supernatant by polyamide column chromatography, eluted by double-distilled water, ethyl acetate precipitated, lyophilized, then the white powder was collected, and demonstrated by cas and arnow assay. the siderophore was phenolate, composed of 2,3-dihydroxybenzoic acid (2,3-dhb), lysine, glycine and tryptophan by hplc. being cultured iniron-deficient medium, the purified siderophore could stimulated the growth of ah n9a which ... | 2000 | 12548885 |
| [gene cloning and expression of prolyl endopeptidase from aeromonas punctata]. | prolyl endopeptidase gene was cloned from aeromonas punctata subsp. punctata(st-78-3-3) using activity screening method and the 3.3 kb dna fragment containing pep gene was sequenced. dna sequence from 20-2092 bp was orf region coding 690 amino acids of prolyl endopeptidase. it was a new pep gene through gene search. the genetic engineered e. coli bl21/pgem-pep overexpressing recombinant aeromonas punctata prolyl endopeptidase(appep) was constructed. cultured in yh medium, the expressed appep was ... | 2000 | 12548992 |
| occurrence of enterotoxigenic aeromonas species in foods. | out of a total of 246 food samples of animal origin screened for isolation of aeromonas spp., 33 (13.41%) were positive for these organisms. maximum positivity was shown by the samples from fish (28.57%), followed by poultry meat (16.67%), poultry eggs (12.50%), goat meat (12%), buffalo meat (7.69%) and cow milk (5.56%). a. hydrophila was the predominant species (51.52%) followed by a. sobria (39.39%) and a. caviae. of these, 70.59% a. hydrophila, 69.23% a. sobria and 33.33% a. caviae showed ent ... | 2000 | 11407001 |
| [pathogenicity factors of various vibrios and aeromonads]. | a review of literature on the pathogenicity factors of non-cholera vibrios and aeromonas is presented. a detailed analysis of such pathogenicity factors as hemolysins, lecithinases, cytotoxins, adhesins, etc. is given. information on some mechanisms of the pathogenetic action of these factors on warm-blooded animals is presented. the necessity of more extensive research on pathogenicity and persistence factors in opportunistic bacteria causing sapronotic infections is emphasized. | 2000 | 11210617 |
| identification and characterization of carnobacteria associated with the gills of atlantic salmon (salmo salar l.). | using the surface plate technique, the population level of aerobic bacteria, occurring in the gills of atlantic salmon (salmo salar l.), was determined to be approximately 3 x 10(4) g(-1). of 100 isolates investigated, 58 were gram-negative. out of the 42 gram-positive isolates, 26 belonged to the carnobacteria, of which ten were further identified on the basis of 16s rdna sequence analysis and aflp fingerprinting. all were identified as carnobacterium piscicola-like. these carnobacteria strains ... | 2000 | 11249022 |
| characterization of oxytetracycline-resistant heterotrophic bacteria originating from hospital and freshwater fishfarm environments in england and ireland. | this ecotaxonomic study compared the antibiotic tolerance among culturable oxytetracyline-resistant (ot(r)) heterotrophic strains isolated from two aquatic environments representing human activities in health care and aquaculture, namely hospital effluents and freshwater fishfarms. using a standardized methodology, samples taken in england and ireland were analyzed to determine the antibiotic tolerance profiles of two groups of culturable ot(r) bacterial isolates at the intergeneric and intragen ... | 2000 | 11249032 |
| norepinephrine as a growth stimulating factor in bacteria--mechanistic studies. | catecholamines (norepinephrine, epinephrine, dopamine) enhance the growth of several species of gram-negative bacteria. since catechol rings are known siderophores in bacteria, the administration of catecholamines may enhance growth by improving iron uptake in growth-limiting media, serving as auxiliary siderophores. we have tested the iron content in bacterial growth media which are known to support rapid growth and "slow growth" media. additionally, we have examined the uptake of 3h-norepineph ... | 2000 | 11125844 |
| beta-lactamase epidemiology and the utility of established and novel beta-lactamase inhibitors. | beta-lactamase inhibitor:beta-lactam combinations remain one of the most successful strategies for the treatment of bacterial infections. over the last 20 years the number and diversity of serine and metallo active site beta-lactamases has increased dramatically. this review highlights some of the new additions to the beta-lactamase arena and discusses how the commercially available beta-lactamase inhibitors are keeping pace with the changing epidemiology of beta-lactamases. in addition, we surv ... | 2000 | 11060675 |
| virulence profiles and other biological characters in water isolated aeromonas hydrophila. | thirty water isolates of a. hydrophila were tested for potential virulence profiles, antibiotic resistance and bacteriocin-like substances (bls) production. cytotoxic activity was present in all strains tested, 87% were hemolytic and 70% adhesive. lysine decarboxylase reactions (ldc) positivity was correlated with virulence factors: 100% versus cytotoxicity, 84% versus adherence, 76% versus hemolytic activity. the correlation was also present in the ldc-negative strains. hemolytic and cytotoxic ... | 2000 | 11061623 |
| incidence and identification of mesophilic aeromonas spp. from retail foods. | sixty-eight food samples were examined for the presence of mesophilic aeromonas species both qualitatively and quantitatively. aeromonads were isolated from 26% of the vegetable samples, 70% of the meat and poultry samples and 72% of the fish and shrimps. numbers of motile aeromonads present in the food samples varied from <10(2) cfu g(-1) to >10(5) cfu g(-1). glc analysis of fames was used to identify a selection of presumptive aeromonas colonies to fenospecies or genomic species level. aeromon ... | 2000 | 11069637 |
| the a-layer influences the susceptibility of aeromonas salmonicida to antibacterial peptides. | 2000 | 11081441 | |
| vaccination influences growth of arctic charr. | there is limited knowledge about the effects of oil-based vaccines on the growth of arctic charr salvelinus alpinus, in particular at different rearing temperatures. one-year-old arctic charr were immunized intraperitoneally at 2.9 degrees c with a metabolizable oil-adjuvanted, bivalent vaccine containing killed typical and atypical aeromonas salmonicida bacteria. after vaccination the non-vaccinated (controls) and vaccinated individually marked fish were held for 20 d at 10.0 degrees c and then ... | 2000 | 11129384 |
| selective enrichment broth for the isolation of aeromonas sp. from chicken meat. | six selective agents (ampicillin, novobiocin, cephalothin, bile salts, brilliant green and ethanol) were tested during the development of a selective enrichment broth for the isolation of aeromonas sp. from food. cephalothin at 10 mg/l was found to be the best selective agent owing to its greater selectivity and efficiency in recovering stressed and lower cell concentrations of aeromonas sp. higher concentrations (15-25 mg/l) of cephalothin were inhibitory to some strains of a. sobria. cephaloth ... | 2000 | 11014523 |
| aeromonas hydrophila isolated from wild freshwater fish in croatia. | aeromonas hydrophila was recovered from fish living in lake vrana on the croatian island of cres. the occurrence of the bacterium in the fish was assessed and related to gross signs of disease and findings at necropsy as a potential health hazard for fish. isolated bacteria were subjected to morphological, physiological, biochemical and antibiotic susceptibility tests. a total of 26 a. hydrophila isolates were obtained. there was a clear seasonality, as no isolates were recovered in the summer m ... | 2000 | 11014606 |
| [isolation of listeria spp., aeromonas spp., and vibrio spp. from seafood products]. | forty-one strains of listeria, aeromonas and vibrio have been isolated in 71 samples of seafood, both raw and ready to eat and frozen. l. monocytogenes, detected by pcr also, is found in the smoked salmon only. aeromonas spp. and vibrio spp. are isolated in the raw products (shrimps and shellfish). no relationship is found between the presence of such microrganisms and the common indicator bacteria. finally, the health hazard related to strong contamination and the need to diversify the food saf ... | 2000 | 11140096 |
| aeromonas hydrophila and its relation with drinking water indicators of microbiological quality in argentine. | in argentine, water municipal supplies disinfection is carried out by chlorine. we have isolated aeromonas hydrophila from a chlorinated water supply in buenos aires that fulfilled argentinean microbiological quality standards. it is an aquatic organism that could produce cytotoxins and enterotoxins associated with acute gastroenteritis and wound infections in human and hemorrhagic septicaemia of fish, reptiles and amphibians. the isolated strain produced protein bands at isoelectric point in th ... | 2000 | 11145418 |
| uptake and processing of biofilm and free-cell vaccines of aeromonas hydrophila in indian major carps and common carp following oral vaccination--antigen localization by a monoclonal antibody. | uptake and processing of biofilm (bf) and free-cell (fc) vaccines of aeromonas hydrophila were studied in the indian major carps catla catla catla, and rohu labeo rohita and in the common carp cyprinus carpio following a single dose oral vaccination of 10(11) cfu g(-1) fish. fish were sampled at 0.5, 1, 3, 6, 12, 24 h and at 2, 3, 5 and 10 d following vaccination and antigen localization was studied in the gut, kidney and spleen employing monoclonal antibody based immunofluorescence and immunope ... | 2000 | 11145450 |
| toxicity of nitric oxide and peroxynitrite to bacterial pathogens of fish. | the inhibitory effect of the nitric oxide (no) donor s-nitroso-acetyl-penicillamine (snap) and the no and o2- donor 3-morpholino-sydnonimine hydrochloride (sin-1) was tested in a cell-free assay. strains of the bacterial fish pathogens aeromonas salmonicida, renibacterium salmoninarum and yersinia ruckeri were exposed to different concentrations of the no donors for 24 h. the results showed that no possesses inhibitory properties, while peroxynitrite had no effect. however, when sin-1 was used i ... | 2000 | 11145451 |
| extended method for discrimination of aeromonas spp. by 16s rdna rflp analysis. | a previously described molecular method, based on 16s rdna rflp analysis, for the identification of aeromonas spp. was unable to separate the species aeromonas salmonicida, aeromonas bestiarum and the recently described aeromonas popoffii. in this study, the method has been extended with endonucleases aiwni and psti for the identification of these species. a molecular frame for the identification of all known aeromonas spp. is presented. | 2000 | 11155981 |
| responses of giant freshwater prawn (macrobrachium rosenbergii) to challenge by two strains of aeromonas spp. | the virulence of two aeromonas strains (a. veronii and a. caviae) isolated from the hepatopancreas of apparently healthy giant freshwater prawns (macrobrachium rosenbergii) was compared using a challenge by injections. for the a. veronii strain, challenge with 3.7 x 10(5) cells/g of body weight led to 100% mortality; for the a. caviae strain, 3.8 x 10(6) cells/g produced 100% mortality. the 50% lethal doses (ld50) were 2.0 x 10(3) cells/g for a. veronii and 51.2 x 10(3) cells/g for a. caviae. us ... | 2000 | 11112373 |
| bacteria associated with processed crawfish and potential toxin production by clostridium botulinum type e in vacuum-packaged and aerobically packaged crawfish tails. | refrigerated vacuum-packaged storage has been shown to increase significantly the shelf life of fresh fish and seafood products, but the effect, if any, on the outgrowth and toxin production of clostridium botulinum type e on cooked crawfish is unknown. microflora associated with live crawfish reflect the microbial populations of the harvest water and sediments in which they are living. the presence or absence of specific pathogens in either vacuum-packaged or air-permeable bags of cooked crawfi ... | 2000 | 11131892 |
| aeromonas caviae as a cause of cholecystitis. | 2000 | 11217277 | |
| [microbiological monitoring of causative agents of sapronoses in the water of the bogatinskoe water reservoir]. | the results of the microbiological monitoring of potential causative agents of sapronoses in the water of the bogatinskoye reservoir revealed that in the summer period of 1998 the mass accumulation of virulent aeromonas sobria (up to 25% of the total number of heterotrophic bacteria) took place. the autumn period was characterized by a decrease in the number of a. sobria and the detection of bacteria of the genus vibrio (up to 22%) with v. mimicus and v. metschnikovii identified among them in th ... | 2000 | 11220958 |
| prevalence and molecular characterization of aeromonas spp. in ready-to-eat foods in italy. | a survey was carried out in italy to ascertain the prevalence of aeromonas spp. in ready-to-eat foods (vegetables, cheeses, meat products, and ice creams) and the level of molecular heterogeneity of the isolates found by macrorestriction analysis of genomic dna with pulsed-field gel electrophoresis (pfge). in total, 46 (14.4%) of the 320 food samples examined were found positive for aeromonas spp. the highest percentages of isolation were discovered in vegetables, particularly lettuce (45.0%), e ... | 2000 | 11131903 |
| surface dynamics of aerolysin on the plasma membrane of living cells. | aerolysin secreted by the human pathogen aeromonas hydrophila belongs to a group of bacterial toxins that are hemolytic and form channels in biological membranes. the toxin is secreted as an inactive precursor proaerolysin that must be proteolytically processed at its c-terminus to become active. the toxin then polymerizes into a heptameric ring that is amphipathic and can insert into a lipid bilayer and form a pore. we have examined these various steps at the surface of target cells. the toxin ... | 2000 | 11111912 |
| re: aeromonas species isolated from medicinal leeches. | 2000 | 11092375 | |
| cytotoxicity assessment of chlorinated bacteria in water using the rna synthesis inhibition method. | chlorination of drinking water containing organic materials is known to generate toxic by-products. we suggested that such compounds may also be produced by interactions between chlorine and bacteria present in water. to confirm this hypothesis, a method based on rna synthesis inhibition of hela s3 human cells in the presence of toxic compounds was applied. this method is rapid and highly sensitive since the concentration of the samples is not required. furthermore, it was shown to be a suitable ... | 2000 | 11090960 |
| production of serine protease of aeromonas sobria is controlled by the protein encoded by the gene lying adjacent to the 3' end of the protease gene. | we cloned a protease gene of aeromonas sobria and determined its nucleotide sequence. the protease is composed of 624 amino acid residues and its calculated molecular weight is 66,737.7. the amino acid sequence showed the characteristic features of a bacterial serine protease. we expressed the protease gene in vibrio parahaemolyticus from which the synthesized protease is secreted into the culture medium as the mature form, and purified the mature protease by successive column chromatographies. ... | 2000 | 11092244 |
| role and behaviour of the hydrophobic conditions in bacterial adhesion to incurrent siphon in a bivalve mollusc. | surface hydrophobicity is a widely distributed characteristic among human bacterial pathogens playing an important role in microbes retention by the incurrent siphon of a marine bivalve. feeding retention experiments with the bivalve mesodesma donacium was done with hydrophobic strains alone (staphylococcus aureus, salmonella paratyphi, vibrio cholerae) or with mixed cultures using a hydrophilic strain (aeromonas hydrophila). results showed that hydrophobic bacteria are retained in greater numbe ... | 2000 | 10997493 |
| production of heat-labile enterotoxin by strains of aeromonas veronii bv veronii. | three isolates of aeromonas veronii bv veronii (2 environmental, one blood infection) were examined to see if they produce any enterotoxin and, if so, to determine its characteristics. two isolates caused fluid accumulation in the initial rabbit ileal loop tests. the other strain did so after a single passage through the rabbit ileal loop. all the isolates showed gradual enhancement of fluid secretion after each subsequent passage. inocula of 1 x 10(4) viable cells and 0.25 ml of culture filtrat ... | 2000 | 11014770 |
| epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. | ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ... | 2000 | 10997832 |
| aeromonas hydrophila infection secondary to an electrical burn. | 2000 | 11008599 | |
| prevalence of enterotoxin genes in aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. | aeromonads are causative agents of a number of human infections. even though aeromonads have been isolated from patients suffering from diarrhea, their etiological role in gastroenteritis is unclear. in spite of a number of virulence factors produced by aeromonas species, their association with diarrhea has not been clearly linked. recently, we have characterized a heat-labile cytotonic enterotoxin (alt), a heat-stable cytotonic enterotoxin (ast), and a cytotoxic enterotoxin (act) from a diarrhe ... | 2000 | 11015403 |
| topically acquired bacterial zoonoses from fish: a review. | the main pathogens acquired topically from fish (through spine puncture or open wounds) are aeromonas hydrophila, edwardsiella tarda, erysipelothrix rhusiopathiae, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and vibrio damsela. s. iniae has recently emerged as a public health hazard associated with aquaculture, and m. marinum often infects home aquarium hobbyists. with the expansion of aquaculture and popularity of recreational fishing in australia, medical practitioners can ex ... | 2000 | 11130351 |
| aerobic intestinal flora of wild-caught african dwarf crocodiles osteolaemus tetraspis. | intestinal contents were collected from wild-caught african dwarf crocodiles (osteolaemus tetraspis) in 1993 and 1995 which were slaughtered at urban markets in the congo republic. the samples were kept frozen and brought back to onderstepoort for aerobic culture. out of 29 specimens, 33 species of bacteria and 20 species of fungi were isolated. the bacteria included three isolates of salmonella and eight isolates of escherichia coli, most of the latter being rough strains. the flora of individu ... | 2000 | 11131121 |
| the expression of proteinases and haemolysins by aeromonas hydrophila under modified atmospheres. | the study estimated the proteolytic activity (against hide powder azure) and haemolytic activity (against horse erythrocytes) in cell-free filtrates (cff) from four strains of aeromonas hydrophila growing under a range of commercially relevant modified atmospheres (2% o2, 78% n2, 20% co2; 10% o2, 80% n2, 10% co2; 50% n2, 50% co2; 100% co2). the examined strains exhibited significant qualitative and quantitative differences in the extent and times of onset of expression of these enzymes under aer ... | 2000 | 11021573 |
| identification of genes associated with copper tolerance in an adhesion-defective mutant of aeromonas veronii biovar sobria. | tnphoa mutagenesis was used to identify adhesins of aeromonas veronii biovar sobria 3767, a strain isolated from a diarrhoeal stool specimen. six mutants, from a library of 154, exhibited significantly reduced levels of adhesion to hep-2 cells. primers to the terminal regions of tnphoa were used for inverse pcr and the product from one mutant was cloned into pbluescript and partial sequence data obtained. scanning genbank and embl data bases revealed dna sequence similarity to the copa gene of p ... | 2000 | 11024350 |
| infections in e-beta thalassemia. | infection is a major complication and the leading cause of death in thalassemia, especially e-beta thalassemia. the spectrum of infections in e-beta thalassemia include mild and severe infections, therapy-related infections such as yersinia enterocolitica infection associated with desferrioxamine (dfo) therapy, and transfusion-transmitted disease, as well as unique infections such as with pythiosis. prospective studies in thailand indicate that patients with e-beta thalassemia had more frequent ... | 2000 | 11132234 |
| [cytoplasmic protein vaccine against bacterial hemorrhagic septicemia (aeromonosis) of fish]. | water-soluble proteins from aeromonas sobria, a causative agent of bacterial hemorrhagic septicemia of fishes, were separated into six fractions by gel chromatography on sephadex g-100. injections of fraction ii (67 kda) provided the highest protection of carps against the disease. injections of proteins contained in fraction ii caused stronger effects on certain biochemical parameters in the fish liver (fatty acids of phospholipids and cathepsin b and d activities) in comparison to infections o ... | 2000 | 11042886 |
| use of a chia probe for detection of chitinase genes in bacteria from the chesapeake bay(1). | pcr primers specific for the chia gene were designed by alignment and selection of highly conserved regions of chia sequences from serratia marcescens, alteromonas sp., bacillus circulans and aeromonas caviae. these primers were used to amplify a 225 bp fragment of the chia gene from vibrio harveyi to produce a chia gene probe. the chia pcr primers and probe were used to detect the presence of the chia gene in an assemblage of 53 reference strains and gave consistent results. selected chia fragm ... | 2000 | 11053737 |
| production and secretion of collagen-binding proteins from aeromonas veronii. | collagen-binding protein (cnbp) synthesized by aeromonas veronii is located conserved within the subcellular fraction. the results of this study show that 98% of the total cnbp produced by aer. veronii is present in the extracellular medium, and that the remaining cnbp is distributed either on the cell surface, within the periplasm or anchored on the outer membrane. cnbp is specifically secreted from aer. veronii into the culture medium, because all the beta-lactamase activity was located in the ... | 2000 | 11054164 |
| reduction of endogenous bacteria associated with catfish fillets using the grovac process. | fresh catfish (ictalurus punctatus) fillets are known to be contaminated with a large number of spoilage and pathogenic bacteria. the grovac method, a new patented (u.s. 5,543,163) process, was evaluated for its efficacy in reducing the number of pathogens and spoilage microorganisms associated with food. this process involves using a processing solution containing ascorbic acid (aa) and sodium chloride (nacl), vacuum, and tumbling. a total of 51 bacterial isolates were isolated and identified f ... | 2000 | 10983798 |
| [purification and characterization of recombinant aeromonas punctata prolyl endopeptidase]. | the study of down-stream techniques of recombinant aeromonas punctata prolyl endopeptidase (appep) was presented here. high cell-density fermentation of e. coli bl21/pkkh-pep in nbs bioflo 3000 5 l fermentor was achieved, the final cell density was 22.5 g (dcw)/l after 14 h cultivation, the yield of appep expressed in soluble protein was 3.0 g per litter broth. after sonication, the supernatant of free cell extract was purified by ammonium sulfate fractionation, high performance q sepharose ff, ... | 2000 | 11059278 |
| functional tn5393-like transposon in the r plasmid pras2 from the fish pathogen aeromonas salmonicida subspecies salmonicida isolated in norway. | tn5393c containing stra-strb was identified as part of r plasmid pras2 from the fish pathogen aeromonas salmonicida subsp. salmonicida. this is the first time an intact and active transposon in the tn5393 family has been reported in an ecological niche other than an agricultural habitat. | 2000 | 11097945 |
| in vitro activity of gemifloxacin (sb-265805) compared with 14 other antimicrobials against intestinal pathogens. | we studied the in vitro activity of gemifloxacin (sb-265805) and 14 comparator antimicrobials against 288 recent isolates of enteropathogenic bacteria (106 salmonella: spp., 32 hafnia alvei, 22 yersinia enterocolitica, 21 shigella: spp., 16 aeromonas: spp. and 91 campylobacter jejuni). gemifloxacin, the other fluoroquinolones and cefotaxime were very active against all microorganisms tested except for c. jejuni. seventy-seven per cent of isolates of c. jejuni were inhibited by erythromycin < or ... | 2000 | 11102426 |
| pulsed-field gel electrophoriesis analyis of aeromonas salmonicida ssp. salmonicida. | a total of 133 strains of aeromonas salmonicida ssp. salmonicida, isolated from a wide variety of sources, were characterized by pulsed-field gel electrophoresis patterns. sixteen profiles were demonstrated, with one profile being predominant in samples from all the countries and species of fish. our results suggest a clonal distribution of this subspecies, with a predominant clone being responsible for most of the outbreaks worldwide. | 2000 | 10981708 |
| denitrification by indigenous microbial populations of river water measured using membrane inlet mass spectrometry. | the major process that reduces nitrate levels in soils and water is denitrification, which converts nitrate and nitrite into gaseous forms of nitrogen, which are then released into the atmosphere. this study used membrane inlet mass spectrometry (mims) to investigate denitrification in river water bacterial isolates supplied with nitrate and succinate as an energy source as well as in the total population by provision of different carbon compounds to untreated river water samples. substantial va ... | 2000 | 10945788 |
| intestinal cell adhesion and maximum growth temperature of psychrotrophic aeromonads from surface water. | investigation of 650 aeromonas strains taken from surface water--used as a source of drinking water--and from the associated drinking water treatment plants has demonstrated that only a small proportion of the strains are endowed with intestinal cell adhesion and growth at 39 degrees c; these characteristics are concurrently encountered in aeromonas strains from clinical material. the investigations permit the conclusion that even the aeromonas species, of which certain strains are pathogenic to ... | 2000 | 10956594 |
| effect of sodium chloride and citric acid on growth and toxin production by a. caviae and a. sobria at moderate and low temperatures. | the effect of sodium chloride and citric acid on hemolysin and caseinase production by aeromonas caviae and aeromonas sobria at 32 degrees c and 5 degrees c was investigated. at 32 degrees c, although both strains were tolerant to 3% nacl in tsb, the production of caseinase was decreased in the presence of 1-3% nacl, and the production of hemolysin was abolished by 2-3% nacl. citric acid (0.03%) was less effective than nacl in reducing hemolysin and caseinase production by both strains at 32 deg ... | 2000 | 11061632 |
| aeromonas hydrophila amph and ceph beta-lactamases: derepressed expression in mutants of escherichia coli lacking creb. | the class 1 cephalosporinase (ceph) and class 2d oxacillinase (amph) from an aeromonas hydrophila clinical isolate, strain t429125, have been cloned and sequenced. both enzymes are typical of their equivalents in other species of aeromonas both cloned beta-lactamase genes were expressed at a low level in a standard laboratory escherichia coli strain, but when cloned into a cre deletion e. coli mutant, they were expressed at significantly higher levels. specific disruption of the creb gene result ... | 2000 | 11062187 |
| inhibition of aeromonas caviae and a. sobria by sodium choloride, citric acid, ascorbic acid, potassium sorbate and extracts of thymus vulgaris. | the respective and combined effects of sodium chloride, ascorbic acid, citric acid, potassium sorbate, and thymus vulgaris extract on the growth of aeromonas caviae and aeromonas sobria were investigated. sodium chloride (3%) significantly reduced the growth and 4% nacl inhibited growth of the tested strains. ascorbic acid (0. 1%), potassium sorbate (0.05%), and citric acid (0.03%) slightly inhibited growth. t. vulgaris extract (0.3%) greatly reduced the growth. various combinations of these com ... | 2000 | 10957708 |
| lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.). | the present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.). a relatively low population level (approximately 2 x 103 bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobac ... | 2000 | 10971765 |
| spontaneous bacterial empyema caused by aeromonas veronii biotype sobria. | spontaneous bacterial empyema is a complication of hepatic hydrothorax in cirrhotic patients. the pathogen, clinical course and treatment strategy are different to the empyema secondary to pneumonia. a 54-year-old man, who was a cirrhotic patient with hepatic hydrothorax, was admitted to national taiwan university hospital for fever, dyspnea and right side pleuritic pain. the image study revealed massive right pleural effusion and no evidence of pneumonia. the culture of pleural effusion yielded ... | 2000 | 10974579 |
| [cultivation optimization of prolyl endopeptidase and high cell density fermentation]. | engineered e. coli bl21/pgem-pep could constitutively express recombinant prolyl endopeptidase from aeromonas punctata, which was extremely effected by culture conditions, so fermentation conditions were optimized to obtain it's high expression level. firstly, the stability of bl21/pgem-pep was investigated, then, culture temperature, ph, time, medium were optimized in shake flaskd. the l9(3(4)) orthogonal experiment confirmed that shaker speed, ph, culture temperature, culture time had high deg ... | 2000 | 10976323 |
| bath exposure of atlantic halibut (hippoglossus hippoglossus l.) yolk sac larvae to bacterial lipopolysaccharide (lps): absorption and distribution of the lps and effect on fish survival. | radiolabelled bacterial lipopolysaccharide (3h-lps) obtained from aeromonas salmonicida subsp. salmonicida was added to the petri dishes containing yolk sac larvae of atlantic halibut (hippoglossus hippoglossus l.). the larvae were exposed either to 6.25, 12.5, 25, 50 or 100 micrograms 3h-lps ml-1. the uptake was both dependent on the lps concentration and the time of exposure. after 5 days of exposure, each larva contained 1.8-7.4 ng 3h-lps dependent on the initial concentration. after 10 days ... | 2000 | 10938728 |
| a putative heat-labile enterotoxin expressed by strains of aeromonas media. | fifteen isolates of aeromonas media (seven from diarrhoeal stools, four from water and four from superficial skin ulcers of catfish) were examined for enterotoxin production. ten of these isolates (six diarrhoeal, one from water and three from fish) caused accumulation of fluid in the initial rabbit ileal loop (ril) tests. isolates from diarrhoeal stools and fish caused relatively more fluid accumulation than those from water. those strains that caused little or no fluid accumulation in the init ... | 2000 | 10933251 |
| isolation and characterisation of a serum lectin from blue gourami, trichogaster trichopterus (pallus). | a novel lectin, designated bgl, has been purified from the serum of blue gourami, trichogaster trichopterus, with the use of (nh4)2so4 fractionation, affinity chromatography and gel filtration chromatography. electrophoretic analyses and mass spectrometric study of purified bgl showed that the lectin is composed of two isoforms with native molecular masses estimated to be 65 and 66 kda, and two subunits of 32 and 34 kda on sds-page under non-reducing conditions. upon reduction with 20 mm dithiot ... | 2000 | 11016584 |
| microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil. | the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ... | 2000 | 11016614 |
| suppression of the humoral immune response of atlantic salmon, salmo salar l. by the 64 kda serine protease of aeromonas salmonicida. | bacteria-free supernatants of broth cultures of aeromonas salmonicida inhibited the humoral immune response, but not the cell-mediated immune response, of atlantic salmon to bacteriophage ms2. the immunosuppressive factor was the 64 kda serine protease secreted by a. salmonicida. the suppressive activity was not due to degradation of epitopes of ms2, and although serine protease degraded the heavy chain of salmon igm in vitro there was no evidence for significant degradation in vivo. the princip ... | 2000 | 10938745 |