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fatal septicaemia caused by aeromonas hydrophila in a patient with cirrhosis.in this case report from italy we describe a fatal infection caused by a. hydrophila in a 39 yr old cirrhotic patient. this pathogen was isolated as a pure single culture from the patient's blood sample. the patient died on the second day of hospitalization from overwhelming sepsis. the a. hydrophila isolate was tested for different potential virulence properties, such as invasiveness, adherence, exotoxins production, presence of fimbriae and for the patterns of resistance to a variety of antimi ...19938281739
aeromonas hydrophila wound infection associated with water immersion: an unusual football injury. 19938288391
enteropathogenic bacteria in river ganges in varanasi.a bacteriological study of the river ganges in varanasi was carried out from december 1985 to november 1987. in all 407 water samples were collected, 335 from bathing ghats and 72 from sewage openings and were examined for the presence of enteropathogenic bacteria. vibrio cholerae 0-1 (1.72%), non 0-1 vibrio cholera (3.69%), vibrio fluvialis (0.74%), aeromonas sp. (0.49%), plesiomonas sp. (0.25%), salmonella sp. (0.98%) and shigella sp. (1.23%) were isolated from both ghats and sewage openings a ...19938157311
multipoint identification of enterobacteriaceae: report of the british society for microbial technology collaborative study.to evaluate the accuracy and reproducibility of multipoint identification schemes in a multicentre trial.19938157751
studies on the hemagglutinant activity of some aeromonas strains.216 strains to aeromonas genus (158 a. hydrophila, 33 a. salmonicida, 23 a. sobria, 1 a. caviae and 1 a. veronii strains) of different sources of isolation were studied concerning their hemagglutinating behaviour to 5 different erythrocyte species (human, bovine, chicken, african green monkey and guinea pig) in the presence/absence of mannose/fucose. the study aimed to establish the spectrum of their hemagglutinating activity and any possible correlation between the source of isolation, biochemi ...19938173123
use of 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies.a new medium, mx-gal, has been developed for the membrane filter enumeration of beta-galactosidase-positive bacteria in municipal water supplies. mx-gal medium contains the chromogenic beta-galactosidase substrate 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (x-gal). all aeromonas, citrobacter, and enterobacter strains isolated from raw water on mx-gal medium were beta-galactosidase positive. in contrast, only 10 to 20% of these strains produced a red colony with a metallic sheen on m-end ...199310755843
indigenous bacteria in hemolymph and tissues of marine bivalves at low temperatures.hemolymph and soft tissues of pacific oysters (crassostrea gigas) kept in sand-filtered seawater at temperatures between 1 and 8 degrees c were normally found to contain bacteria, with viable counts (cfu) in hemolymph in the range 1.4 x 10 to 5.6 x 10 bacteria per ml. pseudomonas, alteromonas, vibrio, and aeromonas organisms dominated, with a smaller variety of morphologically different unidentified strains. hemolymph and soft tissues of horse mussels (modiolus modiolus), locally collected from ...199316348962
4-methylumbelliferyl-beta-n-acetylglucosaminide hydrolysis by a high-affinity enzyme, a putative marker of protozoan bacterivory.hydrolysis of an artificial fluorogenic substrate, 4-methylumbelliferyl-beta-n-acetylglucosaminide, has been studied in a monoculture predator-prey system with either a flagellate (bodo saltans) or a ciliate (cyclidium sp.) fed upon pure bacterial culture (aeromonas hydrophila or alcaligenes xylosoxidans). aeromonas hydrophila produced a low-affinity beta-n-acetylglucosaminidase-like enzyme (k(m), >>100 mumol liter) but alcaligenes xylosoxidans did not. inoculation of both bacterial strains with ...199316349049
thermal inactivation of aeromonas hydrophila as affected by sodium chloride and ascorbic acid.the combined effects of sodium chloride (0, 1.0, 1.5, and 3.0%) and ascorbic acid (0, 1.0, and 2.0 mmol/liter) with mild heat (46 degrees c) on the survival of aeromonas hydrophila were evaluated. because of the nonlinear nature of the survivor curves obtained, several equations yielding an r (coefficient of multiple determination) of congruent with 1 were tested. the equation that most closely fit the curvature of the observed data set was a hyperbolic function. equation coefficients were combi ...199316349116
ci-990 (pd 131112): a new quinolone prodrug.in vitro susceptibility of 1021 strains of recent clinical isolates was determined against the new fluoroquinolone ci-990 (pd 131112) and compared with ci-960, ciprofloxacin, fleroxacin, lomefloxacin, norfloxacin, perfloxacin, sparfloxacin, and temafloxacin. the minimum inhibitory concentrations (mic) of ci-990 in mg/l required for >90% isolates were 0.03-0.5 for members of enterobacteriaceae, 0.12-2.0 for pseudomonas aeruginosa, 0.03-1.0 for aeromonas hydrophilia, 1.0-2.0 for acinetobacter calc ...199317588022
purification and characterization of aeromonas caviae me-1 xylanase v, which produces exclusively xylobiose from xylan.a xylanase, which produces exclusively xylobiose from oat spelt and birch xylans, was isolated from the culture medium of aeromonas caviae me-1. the enzyme (xylanase v) was purified by ammonium sulfate fractionation, hydrophobic interaction, and ion-exchange and gel filtration chromatographies. the homogeneity of the final preparation was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and agarose gel electrofocusing. the molecular mass and isoelectric point of the xyla ...199416349182
an outbreak of aeromonas hydrophila infection in turtles (pseudemis scripta).an outbreak of aeromonas hydrophila infection with a high rate of mortality (95%) in turtles (pseudemis scripta) in italy is reported. pure cultures of the pathogen were isolated from liver, lung, kidney, and heart specimens of the turtles. the a. hydrophila isolate was resistant to amoxicillin, ampicillin, cephalothin, and trimethoprim-sulfamethoxazole but was sensitive to a number of other antibiotics tested. the study indicates that pet turtles can act as reservoirs of this pathogen and may p ...199416349264
siderophore-mediated iron sequestering by shewanella putrefaciens.the iron-sequestering abilities of 51 strains of shewanella putrefaciens isolated from different sources (fish, water, and warm-blooded animals) were assessed. thirty strains (60%) produced siderophores in heat-sterilized fish juice as determined by the chrome-azurol-s assay. all cultures were negative for the catechol-type siderophore, whereas 24 of the 30 siderophore-producing strains tested positive in the csáky test, indicating the production of siderophores of the hydroxamate type. sideroph ...199416349298
identification of aeromonas species isolated from freshwater fish with the microplate hybridization method.aeromonas isolates were obtained from the intestinal tracts of six species of cultured freshwater fish and identified on the basis of their genotypic and phenotypic characters. the microplate hybridization method could differentiate type strains of aeromonas species and related bacteria. dna-dna hybridization analysis showed that 65 aeromonad isolates were 72 to 100% related with either aeromonas caviae, aeromonas hydrophila, aeromonas jandaei, aeromonas sobria, or aeromonas veronii. as many as ...199416349363
a simple and widely applicable method for preparing homogeneous and stable quality control samples in water microbiology.test strains suspended in skim milk, quickly frozen in dry ice-ethanol, and stored at - 70 degrees c can be used as quality control samples that are immediately available by quickly thawing at 37 degrees c. the samples remain homogeneous and stable for at least 1 year, except for aeromonas hydrophila, which decreases 20 to 30% in 1 year.199416349443
transfer in marine sediments of the naturally occurring plasmid pras1 encoding multiple antibiotic resistance.the results of microcosm experiments performed with the fish-pathogenic bacterium aeromonas salmonicida acting as a donor showed that promiscuous plasmid pras1, which encodes tetracycline resistance, is transferred at a high frequency in marine sediments even in the absence of a selective factor. the presence of oxytetracycline resulted in an increase in the transfer frequency compared with that of a microcosm to which no selective factor was added. transfer frequencies of 3.4 x 10 transconjugan ...199416349453
starters in the processing of meat products.starter cultures applied in meat technology may contain moulds, yeasts and bacteria. moulds have been selected with the primary intension to exclude any potential of mycotoxin production. their sensory effects and the requirements of technology are also criteria for suitable starters. their genetic potential can now be modified by genetic engineering. yeasts are applied for sensory reasons mainly. the species in use are only fermentative and do not enhance nitrate reduction. bacterial components ...199422061458
light-emitting aeromonas and plesiomonas generated by transconjugating luxab from escherichia coli.the transposon derivative has been placed on a transposition suicide vector to yield pdb30 in escherichia coli wa803. a simple method, using a tn5 derivative tn5-lux, has been successfully devised for the introduction and stable expression of the bioluminescence property in pseudomonas sp., agrobacterium sp., and rhizobium sp. in this study, there was also successful mating between escherichia coli wa803(pdb30) and strains of acromonas hydrophila and plesiomonas shigelloides. these bacteria emit ...19949747350
enterotoxin-like factors of aeromonas species isolated in kenya.three out of thirteen aeromonas strains tested have a heat-labile enterotoxin-like factor which does not cross-react with cholera toxin serologically, but evokes fluid accumulation in the gut of infant mouse, and causes chinese hamster ovary cell elongation.199412153351
transfer of multiple drug resistance plasmids between bacteria of diverse origins in natural microenvironments.plasmids harboring multiple antimicrobial-resistance determinants (r plasmids) were transferred in simulated natural microenvironments from various bacterial pathogens of human, animal, or fish origin to susceptible strains isolated from a different ecological niche. r plasmids in a strain of the human pathogen vibrio cholerae o1 e1 tor and a bovine escherichia coli strain were conjugated to a susceptible strain of the fish pathogenic bacterium aeromonas salmonicida subsp. salmonicida in marine ...199411865872
antibiotic resistance in clinical and environmental isolates of aeromonas spp. 19948182029
intestinal flora of the medicinal leech hirudinaria manillensis.medicinal leeches are widely used to treat venous congestion in microvascular surgery. aeromonas hydrophila infection, following application of the leech species hirudo medicinalis, is a recognized complication. administration of antibiotics directed at aeromonas has been successful in minimizing complications of infection from this organism. a different leech species, hirudinaria manillensis, has recently been introduced for microsurgical use. a study of the enteric content of 30 of these leech ...19948182569
beta-lactam resistance of motile aeromonas isolates from clinical and environmental sources.the mics of various beta-lactams for 182 isolates of aeromonas species, i.e., a. hydrophila (n = 101), a. sobria (n = 69), and a. caviae (n = 12), from clinical and environmental sources were determined by an agar dilution technique. all strains were resistant to ampicillin and susceptible to aztreonam. a. sobria and a. caviae demonstrated lower resistance rates than a. hydrophila. penicillin-hydrolyzing beta-lactamases were detected in all strains.19948192463
long term survival of a patient with a regressed giant hepatocellular carcinoma after transcatheter hepatic artery embolization (tae) complicated with liver abscess.a 62-year-old male patient with histologically proven hepatocellular carcinoma, received transcatheter hepatic artery embolization (tae) therapy. development of right pyothorax and liver abscess at the tumor region occurred 4 months later after tae. aeromonas hydrophila was isolated from the liver abscess. after repeated percutaneous drainage, the abscess cavity disappeared and the tumor became undetectable by ultrasonography. nineteen months after the initial presentation, a second tumor at the ...19948205501
adherence pattern of non-pilated aeromonas hydrophila strains to tissue cultures.adherence of non-pilated aeromonas hydrophila strains to hep-2, hela, cho and vero tissue culture cells was studied. the strains were isolated from intestinal and extra-intestinal human infections and from the environment. the environmental isolates revealed a diffuse type of adherence to all types of cell lines while clinical isolates showed localized adherence (50%). strains revealing the diffuse type of adherence showed high levels of adhesion i.e. > 20 bacteria per cell. this activity correl ...19948159126
distribution of tetracycline resistance determinants among gram-negative bacteria isolated from polluted and unpolluted marine sediments.tetracycline-resistant gram-negative bacteria were isolated from four different marine sediments in scandinavia and analyzed with dna probes for the determinant classes a to e. colony hybridizations of 429 isolates revealed that class e is the dominating resistance determinant in these marine sediments. comparison of fecally polluted and unpolluted sediments showed few determinant classes in unpolluted sediment and a complex composition of several determinant classes in polluted sediment. total ...19948161183
gram-negative bacillary cellulitis in patients with hepatic cirrhosis.eight episodes of gram-negative bacillary cellulitis in seven patients with hepatic cirrhosis are reported. the patients comprised five women and two men (mean age 59.6 years). the diagnosis was based on a positive culture of specimens obtained by needle aspiration from cutaneous lesions. all patients had grade c cirrhosis according to pugh's classification. cellulitis involved the lower extremities in all cases. five patients developed bullous lesions, three ulcers, two abscesses and two extens ...19948168554
characterization of interfacial catalysis by aeromonas hydrophila lipase/acyltransferase in the highly processive scooting mode.a glycerophospholipid:cholesterol acyltransferase (gcat) that also has lipase activity is secreted by the bacterium aeromonas hydrophila. hydrolysis of the sn-2-ester bond of 1,2-dimyristoyl-sn-glycero-3-phosphomethanol (dmpm) vesicles by this enzyme is shown to occur in a highly processive scooting mode in which the enzyme, substrate, and the products of hydrolysis remain bound to the vesicle interface. this conclusion is based on the following observations. (a) when there is an excess of vesic ...19948172876
[aeromonas spp. as an indicator of the trophic state of bodies of water]. 19948611267
scanning electron microscopy analysis of bacterial colonization in crustaceans. 19948611268
a study of the aetiological agents of childhood diarrhoea in lagos, nigeria.from december 1989 to may 1990, 315 faecal samples from children under 5 years old with diarrhoea (215) and without diarrhoea (100) seen at paediatric clinics were investigated for bacterial, viral and parasitic enteropathogens. standard and recently described methods were used for the investigations, which revealed that 74.9% of children with diarrhoea were infected with enteropathogens compared with 28% of controls. in the diarrhoeal group, 59.1% had a bacterial, 26.5% a viral and 2.3% a paras ...19948289209
adhesion to and invasion of human colon carcinoma caco-2 cells by aeromonas strains.the enteropathogenicity of aeromonas strains that showed mannose-resistant adhesion to int407 cells was evaluated by infecting caco-2 cells and observing them by light and electronmicroscopy. five of six strains adhered in large numbers to caco-2 cells in the presence of mannose and caused cytopathic effects. two strains of aeromonas spp. seemed to invade caco-2 cells, as membrane-bound bacteria were seen within the cytoplasm of these cells; however, staining by acridine orange-crystal violet ap ...19948289215
influence of active site and tyrosine modification on the secretion and activity of the aeromonas hydrophila lipase/acyltransferase.aeromonas sp. secrete a lipase/acyltransferase that shares several properties with the mammalian plasma enzyme lecithin:cholesterol acyltransferase. reaction of the enzyme with tetranitromethane led to modification of 2 tyrosines and a nearly 80% decline in enzyme activity. replacing tyr230 with phe altered the activity of the enzyme in the same way as did treatment with tetranitromethane. unlike the wild type enzyme, which preferentially hydrolyzes the 2-position acyl chain of phosphatidylcholi ...19948294469
aeromonas sobria spontaneous bacterial peritonitis and bacteremia. 19948304329
molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of serratia marcescens that shows imipenem resistance.a clinical isolate of serratia marcescens (tn9106) produced a metallo beta-lactamase (imp-1) which conferred resistance to imipenem and broad-spectrum beta-lactams. the blaimp gene providing imipenem resistance was cloned and expressed in escherichia coli hb101. the imp-1 was purified from e. coli hb101 that harbors psmbnu24 carrying blaimp, and its apparent molecular mass was calculated to be about 30 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. kinetic studies of imp-1 aga ...19948141584
nucleotide sequence of a novel arylesterase gene from vibro mimicus and characterization of the enzyme expressed in escherichia coli.a gene coding for an arylesterase of vibrio mimicus was cloned. sequence determination reveals that the esterase gene has an open reading frame of 600 nucleotides which encodes a protein of m(r) 22,300. the deduced amino acid sequence contain a pentapeptide gdsls (residues 27-31), which was also found in the phospholipid-cholesterol acyltransferase from aeromonas hydrophila. substitution of ser-29 by alanine or cysteine in the cloned gene abolished the esterase activity in the tributyrin plate a ...19948141782
effects of in vitro nickel exposure on the macrophage-mediated immune functions of rainbow trout (oncorhynchus mykiss). 19948142706
assessment of some selective media for the recovery of aeromonas hydrophila from surface waters.a comparison was made of three different growing media already proposed by some authors for the recovery of aeromonas hydrophila in environmental water samples of various origins. the media tested were rippey and cabelli m aeromonas agar, rimler-shotts agar and the ryan aeromonas base medium with the addition of ampicillin. the efficiency of the three media was evaluated on the basis of the following reference criteria: accuracy, selectivity and specificity. the results obtained, analyzed by cal ...19948148018
characterisation of haemolytic activity from aeromonas caviae.aeromonas caviae, an enteropathogen associated with gastroenteritis, displays several virulence characteristics. studies on the kinetics of growth of a. caviae and expression of beta-haemolytic toxin revealed that a. caviae produced maximum haemolytic activity extracellularly during the stationary phase. preliminary studies on the properties of a. caviae haemolysin suggested that divalent cations (mg2+ and ca2+) and thiol compounds, dithiothreitol and mercaptoethanol enhanced the haemolytic acti ...19948150003
mutagenesis of the paracrystalline surface protein array of aeromonas salmonicida by endogenous insertion elements.the tetragonal paracrystalline surface protein array (a-layer) of the fish pathogenic bacterium aeromonas salmonicida is a virulence factor and bacteria which are unable to produce a-layer are attenuated in their ability to kill fish. ten independent mutants of aeromonas salmonicida which were unable to produce a-layer were isolated by growth at 30 degrees c. these mutants displayed either reduced synthesis of the a-layer subunit, synthesis of a truncated subunit, or complete loss of the ability ...19948151705
cloning and expression of putative cytotonic enterotoxin-encoding genes from aeromonas hydrophila.a genomic library from a diarrheal isolate, ssu, of aeromonas hydrophila was constructed in a cosmid vector, phc79, and in bacteriophage lambda embl3. cell lysates from various escherichia coli clones containing the recombinant cosmid were examined for their ability to elongate chinese hamster ovary (cho) cells, which is a typical enterotoxic response. based on restriction analysis, a 4.0-kb sali dna fragment from one of the clones that exhibited enterotoxic activity was subcloned into a bacteri ...19948112594
cytotoxic and haemagglutinating activities of motile aeromonas species.cytotoxic and haemagglutinating properties were determined in 114 aeromonas strains isolated from various sites in slaughtered lambs and from processed lamb meat. cytotoxic activity on vero cells was observed in 48 (42%) of the strains. it was more common in a. sobria and a. hydrophila isolates than with a. caviae isolates. haemagglutination (ha) activity was found frequently in motile aeromonads irrespective of species; it was present in 50% of a. sobria strains, 51% of a. hydrophila strains an ...19948114068
crystal structure of aeromonas proteolytica aminopeptidase: a prototypical member of the co-catalytic zinc enzyme family.aminopeptidases specifically cleave the amino-terminal residue from polypeptide chains and are involved in the metabolism of biologically active peptides. the family includes zinc-dependent enzymes possessing either one or two zinc ions per active site. structural studies providing a detailed view of the metal environment may reveal whether the one-zinc and two-zinc enzymes constitute structurally and mechanistically distinct subclasses, and what role the metal ions play in the catalytic process ...19948087555
molecular cloning and characterization of prolyl endopeptidase from human t cells.the prolyl endopeptidase (pep) gene of human t cells was amplified by the pcr method and cloned in escherichia coli. the complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. the nucleotide sequence of this clone revealed that t cell pep dna is 48, 50, and 91% homologous to those of flavobacterium meningosepticum, aeromonas hydrophila, and porcine brain pep, respectively. this gene was fused to the lacz sequence from e. ...19948089089
assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of aeromonas sp isolates.to assess a chemiluminescent universal probe for taxonomical and epidemiological investigations of aeromonas sp isolates.19948089222
in-vitro evaluation of cefpodoxime.in-vitro antimicrobial activity of cefpodoxime was evaluated against several microbial species by both conventional tests and additional parameters which take into consideration some of the conditions likely to be encountered in infected tissues. mics for 414 recent clinical isolates, including staphylococci, streptococci, haemophilus influenzae, moraxella catarrhalis, several enterobacteriaceae, aeromonas hydrophila and campylobacter jejuni were determined. mic values overall were similar to th ...19948040114
diversity of siderophore genes encoding biosynthesis of 2,3-dihydroxybenzoic acid in aeromonas spp.most species of the genus aeromonas produce the siderophore amonabactin, although two species produce enterobactin, the siderophore of many enteric bacteria. both siderophores contain 2,3-dihydroxybenzoic acid (2,3-dhb). siderophore genes (designated aebc, -e, -b and -a, for aeromonad enterobactin biosynthesis) that complemented mutations in the enterobactin genes of the escherichia coli 2,3-dhb operon, entceba(p15), were cloned from an enterobactin-producing isolate of the aeromonas spp. mappin ...19948043988
[activity of lysosomal proteinases in carb tissue in aeromonas infection].the activity of major lysosomal proteinases (cathepsins b and d) increased in carp tissues infested with aeromonas hydrophila. the value and character of changes in the activity of cathepsins depended on the degree of infestation and the physiological state of fishes (normally fed or starved).19948047538
isolation and characterization of a tn5-induced tolq mutant of escherichia coli.transposon mutagenesis was used to isolate an escherichia coli mutant that released into the external medium a heterologous protein, the aeromonas hydrophila aerolysin. genetic mapping and phenotypic characterization of e. coli cm209 showed that tn5 is inserted in the tolq gene. this mutant strain released a significant amount of unprocessed (proaerolysin) protein into the external medium, together with other periplasmic proteins. similar levels of the toxin were detected in the intracellular co ...19948050069
the transposon-like structure of is26-tetracycline, and kanamycin resistance determinant derived from transferable r plasmid of fish pathogen, pasteurella piscicida.tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable r plasmid of fish pathogen pasteurella piscicida, and complete nucleotide sequences were determined. the pp-tet was a class d tet determinant constructed with the teta resistance gene of 1,182 bp encoding a protein with a deduced molecular mass of 41 kda and the tetr repressor gene of 654 bp encoding a product of 24 kda. the pp-tet was highly homologous to the tet(d) of plasmid ra1 isolated from aeromo ...19948052160
aeromonas peritonitis.five new cases of peritonitis caused by aeromonas species are reported, and 29 others described in the literature are reviewed. males predominated (71%), and the mean age was 56.9 years. acquisition was nosocomial in 20% of the cases. all patients except one (3%) had significant underlying diseases; 73% had chronic hepatic disease, 15% had chronic renal failure (treated with chronic ambulatory peritoneal dialysis [capd]), and 9% had an intestinal perforation. symptoms were similar to those of pe ...19948054435
[aeromonads and their sensitivity to antibacterial drugs (review of the foreign literature 1985-1992)].the review is concerned with the modern studies on the taxonomy of aeromonades and their role in the development of infectious complications of various localization, including intestinal infections. a special attention is paid to antibiotic resistance of aeromonades isolated from pathological materials and the modern notions of the factors of aeromonade pathogenicity.19948060198
dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis.immunostimulant preparations macrogard, candida utilis, saccharomyces cerevisiae, evetsel, chitosan, or finnstim were mixed into semipurified diets and fed to groups of rainbow trout for 1 week. fish were bled by non-lethal caudal puncture and blood samples assayed for changes in non-specific cellular immunity and humoral protein levels. in the immunostimulated fish, hematocrit levels and lymphocyte counts remained relatively stable; however, elevations were observed in oxidative radical release ...19948066989
a beta-glucan inhibitable zymosan receptor on channel catfish neutrophils.in mice and humans zymosan binds to the complement receptor three/mac-1 receptor; however, identification of this receptor in channel catfish (ictalurus punctatus) has not been accomplished. soluble fluorescein isothiocyanate (fitc) conjugated beta-glucan, a component of zymosan, was found to bind to channel catfish anterior kidney (ak) neutrophils but not to b-lymphocytes. serum activated zymosan (saz)-mediated chemiluminescence responses of channel catfish ak neutrophils could be inhibited by ...19948066990
adaptive acid tolerance response (atr) in aeromonas hydrophila.aeromonas hydrophila, a gastrointestinal pathogen of humans, was shown to exhibit a significant adaptive acid tolerance response (atr) capable of protecting cells from severe acid at a ph of 3.5. the atr was induced by exposure to a relatively mild ph level of 5.0 for 20 min. adaptation required protein synthesis since treatment with chloramphenicol during adaptation to ph 5.0 prevented the development of acid tolerance. the adaptation to acid environment was found to be a non-transient phenomen ...19948075809
two-year study of endemic enteric pathogens associated with acute diarrhea in new caledonia.a longitudinal study of diarrheal disease among patients of all ages with acute diarrhea was carried out in new caledonia from january 1990 to december 1991. stool samples from 2,088 diarrheal patients were examined for parasites, rotavirus, and bacterial pathogens. potential sources of contamination (drinking water, seawater and bovine and porcine feces) were investigated. one or more enteric pathogens were identified in 41.8 and 40.6% of the persons with diarrhea, in 1990 and 1991, respectivel ...19948077399
rapid biochemical screening for salmonella, shigella, yersinia, and aeromonas isolates from stool specimens.four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. the salmonella screen consisted of kligler iron agar (kia), motility-indole-urea-tryptophan-deamination semisolid medium (miu-tda), and the o-nitrophenyl-beta-d-galactopyranoside (onpg) test; the shigella screen consisted of kia, miu-tda, the onpg test, and the lysine decarboxylation-indole test; the yersinia screen consisted of a rhamnose broth; the aeromonas screen consisted of ...19948077408
comparison of the microbiological quality of water coolers and that of municipal water systems.the microbiological quality of tap water and that of water from 50 water coolers located in residences and workplaces were comparatively studied. in addition, difference factors that might influence the bacteriological contamination of water dispensers were examined. aeorbic and facultative anaerobic heterotrophic bacteria, total coliforms, and two indicators for fecal contamination (fecal coliforms and fecal streptococci) as well as three types of pathogenic bacteria (staphylococcus aureus, pse ...19948017912
comparison of putative virulence factors in aeromonas hydrophila strains isolated from the marine environment and human diarrheal cases in southern italy.aeromonas hydrophila strains isolated from the same geographical region (southern italy) but from different sources (sea sediments and human diarrhea cases) were characterized for the production of potential virulence determinants, such as production of cytotoxins, cytotonic toxins, hemolysin, and dermonecrotic factors and their capacity to adhere to human intestinal 407 cells in vitro. the results showed that isolates from both the sources produced all or some of the virulence factors which may ...19948017924
sequence analysis of the l1 metallo-beta-lactamase from xanthomonas maltophilia.the amino acid sequence deduced from the l1 beta-lactamase gene of xanthomonas maltophilia shows a significant variation from that of the cpha and blm metallo-beta-lactamases of aeromonas hydrophila and bacillus cereus, respectively. whilst the n-terminus of the l1 protein shows some similarity, particularly at the histidine residues previously suggested as a zinc-binding motif, the c-terminus of the protein demonstrates very little similarity. such differences amongst this group of enzymes woul ...19948018721
[biochemical identification of aeromonads].to assess the species of the genus aeromonas in 178 strains isolated from human and animal materials and from the environment (water and hospital environment), examined along with 11 type strains of the best known species, a key was used the basis of which are in addition to biochemical tests defining the genus aeromonas 12 tests: voges-proskauer, lysine decarboxylase, gas from glucose, haemolysis, gluconate oxidation, elastase, arabinose, mannose, saccharose, salicine, esculine hydrolysis, arbu ...19948019811
[identification of aeromonads from water sources].a total of 102 aeromonas strains isolated from water were identified by both commercial enterotest 1 & 2 kits and by several conventional tests. 83 strains (81.4%) were identified to the species level according to a differentiation table. a. hydrophila (36 strains), a. caviae (26 strains) and a. sobria (10 strains) species were isolated most frequently. strains identified as a. allosaccharophila, a. eucrenophila, a. jandaei, a. media and a. trota were very rare. the remaining 19 strains could no ...19948019812
survival and recovery of aeromonas hydrophila in water: development of methodology for testing bottled water in canada.proposed changes to the regulations for bottled water in the food and drugs act of canada include criteria for aeromonas hydrophila (0 colony-forming units/100 ml water). the development of the methodology used to support these proposed regulations and the survival of a. hydrophila in inoculated water are described. the methodology used in the isolation of a. hydrophila includes the use of hydrophobic grid membrane filters (hgmf), a resuscitation step on tryptic soy agar, and selective plating o ...19948019937
aeromonas species exhibit aggregative adherence to hep-2 cells.clinical and environmental isolates of aeromonas species (five a. hydrophila isolates, three a. caviae isolates, and two a. sobria isolates) were tested for their adherence to hep-2 cells. clinical isolates of a. hydrophila and a. sobria exhibited aggregative adherence similar to that presented by enteroadherent-aggregative escherichia coli. bacterial aggregates adhered to cells with a typical "stacked-brick" appearance. in contrast, a. caviae strains showed a diffuse adherence pattern.19948027331
colonic carcinoma manifesting as aeromonas colitis. 19948034927
the susceptibility of s-layer-positive and s-layer-negative aeromonas strains to complement-mediated lysis.forty strains of aeromonas hydrophila and aeromonas veronii recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (phs). based upon the results of this assay, two major populations could be defined. the first group (n = 20) consisted of serogroup o:11 strains, all of which possessed a paracrystalline surface layer (s layer); all of these strains were refractory to the bactericidal activity of 65% phs with t ...19948000552
characterization of aeromonas genomic species by using quinone, polyamine, and fatty acid patterns.a chemotaxonomic study was carried out on representative strains of 13 aeromonas genomic species. quinone, polyamine, and fatty acid patterns were found to be very useful for an improved characterization of the genus and an improved differentiation from members of the families enterobacteriaceae and vibrionaceae. the q-8-benzoquinone was the predominant ubiquinone, and putrescine and diaminopropane were the major polyamines of the genus. the fatty acid patterns of 181 strains, all characterized ...19948000962
differential elimination of enteric bacteria by protists in a freshwater system.the short-term (1 h) and long-term (3 d) elimination of low and high densities of five enteric bacteria, klebsiella pneumoniae, aeromonas hydrophila, escherichia coli, enterococcus faecalis and staphylococcus epidermidis, by flagellate and ciliate protists were measured in a freshwater system. in addition, the two processes, ingestion and digestion, which cause the disappearance of those enteric bacteria as time passes, were quantified. the results showed that the elimination of these enteric ba ...19948002473
characteristics of 'atypical', cytochrome oxidase-negative aeromonas salmonicida isolated from ulcerated flounders (platichthys flesus (l.)).'atypical', cytochrome oxidase-negative variants of the fish pathogen aeromonas salmonicida, isolated from ulcerated flounder (platichthys flesus), were studied using different methods. two of the strains possessed a protein that corresponded to the a-layer protein of aer. salmonicida. the strains reacted with antibodies against the a-layer and monoclonal antibodies against the o-antigen of typical aer. salmonicida. these tests confirm that the isolates from flounder should be classified as aer. ...19948005836
[sensitivity of different species from the mesophilic aeromonas group isolated from feces to different ph values. clinical significance in the pediatric population]. 19948011731
empyema due to aeromonas hydrophila. 19948011840
maltose transport in aeromonas hydrophila: purification, biochemical characterization and partial protein sequence analysis of a periplasmic maltose-binding protein.a clinical isolate of aeromonas hydrophila was demonstrated to transport [14c]maltose with similar kinetics to enteric bacteria (km: 0.3 microm; vmax: 22 nmol min-1 per 10(9) cells). the uptake of [14c]maltose was completely inhibited in the presence of unlabelled maltose or maltodextrins, whereas other mono- and disaccharides, such as glucose, galactose, sucrose, lactose or melibiose, had no effect. a protein with an apparent molecular mass of 39 kda (maltose-binding protein; mbp) was identifie ...19948012611
effect of growth temperature on complement-mediated killing of mesophilic aeromonas spp. serotype o:34.mesophilic aeromonas spp. strains (serotype o:34) showed sensitivity to complement-mediated killing when they were cultivated at 37 degrees c (serum-sensitive) but not when they were cultivated at 20 degrees c (serum-resistant). these strains produced smooth lipopolysaccharide when they were grown at 20 degrees c and rough lipolysaccharide when cultivated at 37 degrees c. the reason for the resistance to complement-mediated killing could be that c3b is rapidly degraded (possibly because it is bo ...19948013874
production of dogik: an improved ogi (nigerian fermented weaning food) with potentials for use in diarrhoea control.as part of a programme to formulate foods to aid the control of diarrhoeal diseases, an improved ogi (the commonest weaning food in nigeria) named dogik has been developed. dogik was produced by using lactobacillus starter cultures with antimicrobial activity against diarrhoeagenic bacteria and also possessing amylolytic activity. the survival of diarrhoeagenic bacteria was investigated in locally-fermented ogi and in dogik. the foods were inoculated with cell suspensions of salmonella, shigella ...19948015024
diarrheal disease in peru after the introduction of cholera.surveillance was conducted one day each week from december 1992 through may 1993 to determine the clinical features and etiology of diarrhea among a population in a suburban community of lima, peru. patients who had had three or more loose stools during the previous 24 hr were enrolled at a clinic located in the community or at a nearby regional hospital. a total of 143 cases of diarrhea were detected for an overall rate of 7.1 cases per 1,000 population. the enteropathogens isolated were vibrio ...19947985750
resistance of bacterial strains to dry conditions: use of anhydrous silica gel in a desiccation model system.the viability of 18 bacterial strains desiccated on anhydrous silica gel and stored at a temperature of 22 degrees c for at least 3 months was determined. according to their stability in the dried state, these strains could be classified into three typical groups. group 1, containing gram-positive strains and salmonella serotypes, was marked by a very slow decrease of the concentration of culturable cells from day 14 on (respectively day 21 for salmonella thompson). the rate of decrease expresse ...19947989258
the vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms.the physiological significance of the occurrence of sequence similar antimicrobial peptides in frog skin, as the bombinins in bombina, the magainins in xenopus, and the dermaseptins in phyllomedusa, is a major unanswered question. dermaseptins s1, s2, s3, s4, and s5, a family of cationic (lysine-rich), amphipathic antifungal peptides of 28-34 residues were thus synthesized, purified to homogeneity, and evaluated for their growth-inhibition activity in vitro against various pathogenic microorgani ...19947989335
atypical aeromonas salmonicida isolated from ulcerated chub leuciscus cephalis. 19947992477
three beta-lactamases isolated from aeromonas salmonicida, including a carbapenemase not detectable by conventional methods.the beta-lactamases of seven strains of aeromonas salmonicida subsp. achromogenes resistant to amoxicillin (mic > 1024 mg/l) and responsible for furunculosis in farmed atlantic salmon in scotland were examined to establish the mechanisms of beta-lactam resistance. separation of a cell-free extract on an isoelectric focusing gel stained with the chromogenic cephalosporin nitrocefin showed the presence of two beta-lactamases, one with a pi of 7.9 and the other with a pi of 6.0. hydrolysis assays o ...19947889949
prolyl aminopeptidase is not a sulfhydryl enzyme: identification of the active serine residue by site-directed mutagenesis.prolyl aminopeptidase (pap) has been classified as a sulfhydryl enzyme on the basis of its high sensitivity to p-chloromercuribenzoate and heavy metals. recently, however, the possibility of pap being instead a serine enzyme has been raised as a result of two observations--the conservation of some residues among the paps hitherto sequenced, and a similarity to some serine hydrolases. this is the first report describing an attempt to identify the active residue by site-directed mutagenesis. the p ...19947896753
[distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms].the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ...19947898396
conditions for the production and detection of aeromonas enterotoxins. 19947885245
phagocytic defence mechanism in sea bass (dicentrarchus labrax l.): an ultrastructural study.the ultrastructure of the phagocytic process in fish has not been established in spite of the significant morphofunctional differences detected in the fish immune system with respect to the basic immunological pattern in vertebrates. we report the ultrastructure of the bacterial phagocytic defence mechanism in sea bass (dicentrarchus labrax l.).19947879910
aeromonas caviae exhibits aggregative adherence to hep-2 cells. 19947880270
isolation and characterization of the prolyl aminopeptidase gene (pap) from aeromonas sobria: comparison with the bacillus coagulans enzyme.the aeromonas sobria pap gene encoding prolyl aminopeptidase (pap) was cloned. it consists of 425 codons and encodes a homotetrameric enzyme of 205 kda. the purified enzyme showed an almost absolute specificity for amino-terminal proline. proline and hydroxyproline residues from many peptide and amide substrates could be easily removed, while no activity was detected for substrates having other amino terminals. the enzyme was very similar to that from bacillus coagulans in many aspects, such as ...19947883756
case of aeromonas veronii (dna group 10) bacteremia.we describe the first case report of bacteremia due to aeromonas veronii biotype veronii. the infection occurred in a 77-year-old man suffering from multiple underlying conditions which included cancer of the sigmoid colon. because of the unusual biochemical phenotype of this group (ornithine decarboxylase positive), it was originally identified as vibrio cholerae.19947883912
physiological consequences of the s-layer of aeromonas salmonicida in relation to growth, temperature, and outer membrane permeation.s-layers are paracrystalline protein multimers that cover the entire cell surface of many bacterial species. the presence of an s-layer in aeromonas salmonicida (also known as a-layer) predisposed this bacterium to apparently unrelated physiological consequences: inhibition of growth at 30 degrees c, enhanced cell filamentation at 37 degrees c, and enhanced uptake of the hydrophobic antibiotics streptonigrin and chloramphenicol. growth inhibition or enhanced filamentation was not observed when t ...19947922885
toxin production, adherence and protein expression by clinical aeromonas spp. isolates in broth and human pooled ileostomy fluid.the physiological behaviour of clinical aeromonas spp. isolates was compared following culture in a conventional broth and human pooled ileostomy fluid (pif). protein expression was markedly affected by the growth medium, with an overall reduction in whole cell proteins in bacteria grown in ileostomy fluid. in addition, novel outer membrane proteins were produced in pif but not in broth. the majority of a. hydrophila and a. sobria isolates produced toxin in both broth and pif, whereas no cytotox ...19947925662
etiology and outcome of diarrhea after marrow transplantation: a prospective study.acute diarrhea after marrow transplant is usually ascribed to acute graft-vs.-host disease (gvhd) or infection, with a reported 40%-50% incidence of infection. the aim of this study was to determine the incidence of acute diarrhea after transplantation, its causes, and its outcome.19947926504
characterization of aeromonas salmonicida subsp. salmonicida: a comparative study of strains of different geographic origin.a total of 130 strains of the fish pathogen aeromonas salmonicida isolated in denmark, norway, scotland, canada and the usa were examined. the strains originated from farmed salmonid fish. the biochemical, physiological and serological characteristics, antibiotic resistance patterns and cell surface-related properties were compared. aeromonas salmonicida was found to be remarkably consistent in general cultural and biochemical characteristics. it is noteworthy that the strains were positive in t ...19947928780
evaluation of different antimicrobial agents used as selective supplements for isolation and recovery of yersinia enterocolitica.seventeen antimicrobial agents were evaluated separately or in combination for their efficiency as selective supplements in a broth medium against six different serotypes of yersinia enterocolitica and 20 selected strains of different gram-negative bacteria. irgasan (dp300, 5-chloro-2-(2,4- dichlorophenoxy) phenol) at a concentration of 4 micrograms ml-1 inhibited the growth of most gram-negative bacteria with the exceptions of aeromonas hydrophila, morganella morganii, pseudomonas aeruginosa an ...19947928783
factors influencing beta-galactosidase activity of aeromonas caviae.aeromonas caviae, often reported to be associated with diarrhoeal patients, elaborates several virulence factors as well as catabolic enzymes such as xylanase and beta-galactosidase. studies on the kinetics of growth of a. caviae and synthesis of beta-galactosidase suggested that the activity was cell associated and reached a peak during the late logarithmic phase of growth. the optimum ph for beta-galactosidase activity was 7.0 and required ca2+ and glutathione for enhancement of its activity; ...19947932108
isolation of aeromonas salmonicida from paddlefish, polyodon spathula.aeromonas salmonicida was isolated from paddlefish (polyodon spathula) mortalities collected during an epizootic of furunculosis at the spring river state hatchery, arkansas (usa), in 1992. isolates of the bacterium were obtained from culture of gill and kidney tissue. this is the first epizootic of bacterial etiology to be reported in paddlefish.19947933294
unique fimbriae-like structures encoded by sefd of the sef14 fimbrial gene cluster of salmonella enteritidis.the sef14 gene cluster of salmonella enteritidis was recently shown to contain three genes, sefabc, encoding a unique fimbrin, and proteins homologous to fimbrial chaperones and outer membrane proteins (ushers), respectively. a fourth open reading frame, designated sefd, was found immediately downstream of sefabc and overlapping sefc. the translated protein sequence of sefd was unique, but the composition was similar to that of other bacterial fimbriae. sefd was produced in abundance by wild-typ ...19947934897
spring viraemia of carp. 19947941273
genetic variation in the humoral immune response in atlantic salmon (salmo salar) against aeromonas salmonicida a-layer.antibody responses to aeromonas salmonicida a-layer were analysed in family material of atlantic salmon (salmo salar), consisting of 791 fish belonging to 34 full-sib groups within 12 paternal half-sib groups. the fish were immunized twice and blood samples were collected three times. significant increase in antibody titre from first to second, and from second to third sampling, was observed. genetic variation in antibody titres was observed at the three samplings with estimated heritabilities r ...19947941312
aeromonas infection and intramural intestinal hemorrhage as a cause of small bowel obstruction. 19947942696
corneal ulcer caused by aeromonas species. 19947943138
aeromonas species in septicemia: laboratory characteristics and clinical observations.we retrospectively analyzed clinical and epidemiological data on and laboratory characteristics of 53 cases of aeromonas septicemia. only four aeromonas genomospecies (species defined by dna relatedness) were associated with the 53 cases, with aeromonas hydrophila (sensu stricto) predominating (47%). nearly 60% of all aeromonas isolates from blood fell into one of four somatic groups: serogroups o:11, o:16, o:18, and o:34. unlike aeromonas-associated gastroenteritis, septicemia did not peak in f ...19947948561
in-vitro activity of four new fluoroquinolones.the in-vitro activities of four new fluoroquinolones, e-4749, e-4874, e-4884 and e-4904, were compared with that of ciprofloxacin and sparfloxacin against 1106 clinical isolates. against majority of enterobacteriaceae, general antibacterial activities of e-4749 (mic90s 0.06-1 mg/l), e-4874 (mic90s, 0.03-0.25 mg/l) and e-4884 (mic90s 0.01-0.5 mg/l) were comparable or slightly lower than those of ciproloxacin (mic90s 0.01-0.25 mg/l) and sparfloxacin (mic90s 0.01-1 mg/l). the activity of e-4904 (mi ...19947961215
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