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growth and survival of clinical vs. environmental species of aeromonas in tap water.the ability of four species of aeromonas (two of clinical and two of environmental origin) to survive and/or grow in tap water microcosms supplemented with sodium thiosulphate was tested. after bottling, the autochthonous microflora reached 6 x 10(5) cfu ml(-1) after a 5-day incubation period in tap water unfiltered and which was non-autoclaved. in filtered tap water, "ultramicrocells" were detected and final populations of ca. 10(6) cfu ml(-1) after 7 days were obtained. aeromonas was inoculate ...200111603856
epidemiology, clinical course and impact on hospitalization costs of acute diarrhea among hospitalized children in athens, greece.the aim of this study was to describe the etiology, morbidity and hospitalization costs associated with acute diarrhea among hospitalized children in greece. during 1999, 294 hospitalized children (median age 1 y) with acute diarrhea were prospectively studied. bacterial and viral enteropathogens were detected in 100 (34%) and 37 (12.5%) patients, respectively; 17 (6%) patients had mixed infections. isolated agents included salmonella spp. (43 patients; 15%), rotavirus (32; 11%), campylobacter s ...200111669226
three cases of bacteraemia caused by aeromonas veronii biovar sobria.septicaemia caused by aeromonas species is a life-threatening condition, arising primarily in immunocompromised patients, which has rarely been reported in scandinavia. herein we describe 3 cases of aeromonas sobria bacteraemia from denmark. all the patients were male and all 3 cases occurred during the summer. two patients had acute leukaemia and hiv infection, respectively, while the third patient had colorectal cancer diagnosed several years later. the clinical presentation in all patients wa ...200111669236
acute diarrhoea in children treated in an outpatient setting in athens, greece.to describe the epidemiology and aetiology of acute diarrhoea among children treated exclusively in an outpatient setting in greece.200111676518
identification and characterization of the three chitin-binding domains within the multidomain chitinase chi92 from aeromonas hydrophila jp101.the gene (chi92) encoding the extracellular chitinase of aeromonas hydrophila jp101 has been cloned and expressed in escherichia coli. the mature form of chi92 is an 842-amino-acid (89.830-kda) modular enzyme comprised of a family 18 catalytic domain, an unknown-function region (the a region), and three chitin-binding domains (chbds; chi92-n, chbd(ci), and chbd(cii)). the c-terminally repeated chbds, chbd(ci) and chbd(cii), were grouped into family v of cellulose-binding domains on the basis of ...200111679332
[aeromonas species infection with severe clinical manifestation in okinawa, japan -association with gas gangrene-].we experienced two patients having aeromonas species infection with severe clinical manifestations. the one patient was a 15-year-old high school girl student, who had been healthy in her school life, was admitted to the hospital with a sudden onset of left thigh muscle pain and swelling. she subsequently went into septic shock and died one day after admission. pathological examination on autopsy revealed massive gas formation, skin bullas and ulcers, and extensive severe soft tissue damage thro ...200111682002
numerical taxonomy of microorganisms isolated from goat cheese made in chile.118 strains of heterotrophic microorganisms were isolated from goat cheese produced domestically in the iv region of northern chile (serene, ovalle, and illapel) and sold in supermarkets in valparaíso, chile. the results of 89 phenotypic tests were numerically analyzed against 17 reference strains, using the simple matching coefficient (s(sm)). thirteen phena were found at a 78% similarity level. five of them (a, b, c, d, and e) were assigned to the family enterobacteriaceae, phenon f was identi ...200111685504
aerolysin from aeromonas hydrophila and related toxins. 200111417121
immunological parameters of javanese carp puntius gonionotus (bleeker) exposed to copper and challenged with aeromonas hydrophila.this study was to determine the median lethal concentration (lc50) of copper to javenese carp, puntius gonionotus (bleeker), and the immune response after the fish were exposed to sublethal levels of copper and challenged with formalin killed aeromonas hydrophila. the lc50 of copper on p. gonionotus at 24, 48, 72, 96 and 120 h were estimated as 2.17, 0.91, 0.57, 0.53 and 0.42 mg l(-1), respectively. to determine the effect of copper on the immune system, fish were exposed for 66 days to 0.05, 0. ...200111417716
effect of ascorbic acid on the immune response of the catfish, mystus gulio (hamilton), to different bacterins of aeromonas hydrophila.ascorbic acid is one of the essential vitamins for normal physiological activities of any organism. the present study demonstrates an immunostimulatory effect of vitamin c on the humoral and cell mediated immunity of the bagrid catfish, mystus gulio, determined using different bacterins of aeromonas hydrophila. humoral as well as cell mediated immune responses were elucidated in the vitamin supplemented, vaccinated and unvaccinated groups. the vitamin supplemented lipopolysaccharide (lps) vaccin ...200111417721
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
detection of rtx toxin gene in vibrio cholerae by pcr.a pcr that amplifies a recently discovered vibrio cholerae rtx (repeat in toxin) toxin gene was developed. among 166 clinical and environmental isolates of v. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both pcr and hep-2 cell cytotoxicity assay. standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. this is the first rapid genotyping method fo ...200111427575
characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon.this study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: lactobacillus sake, l. farciminis, l. alimentarius, carnobacterium piscicola, aeromonas sp., shewanella putrefaciens, brochothrix thermosphacta, photobacterium phosphoreum and enterobacteriaceae isolated from cold-smoked salmon. each bacterial group was represented by several strains. in addition, combinations of the groups were examined as well. sterile blocks of cold-smoked salmon ...200111428576
using light scatter signal to estimate bacterial biovolume by flow cytometry.in the past decade, flow cytometry has become a useful and precise alternative to microscopic bacterial cell counts in aquatic samples. however, little evidence of its usefulness for the evaluation of bacterial biovolumes has emerged in from the literature.200111429769
the cell division genes (ftse and x) of aeromonas hydrophila and their relationship with opsonophagocytosis.a transposon mutant from aeromonas hydrophila ah-3 was obtained which was highly resistant to opsonophagocytosis. the mutation was identified in the ftse gene and we characterised the operon ftsy, e and x from this bacterium. these genes, as in enteric bacteria, are neighbours to rpoh. the a. hydrophilia ftse and x genes were fully able to complement escherichia coli ftse mutants, and also complement the opsonophagocytosis-resistant phenotype of the a. hydrophila mutant strain. this phenotype se ...200111430412
the mgte mg2+ transport protein is involved in aeromonas hydrophila adherence.aeromonas hydrophila ah-3 strains carrying mutations in mgte, which encodes a mg2+ and co2+ transport system, showed a 50% reduction of in vitro adherence to hep-2 cells, a reduction in swarming in semisolid swarming agar, and decrease in biofilm formation of over 60% in comparison to the wild-type strain. the cloned a. hydrophila mgte expressed from a plasmid complements a salmonella typhimurium strain deleted for all mg2+ transporters both phenotypically and by measurement of 57co2+ uptake. li ...200111430413
inactivation of aeromonas hydrophila metallo-beta-lactamase by cephamycins and moxalactam.incubation of moxalactam and cefoxitin with the aeromonas hydrophila metallo-beta-lactamase cpha leads to enzyme-catalyzed hydrolysis of both compounds and to irreversible inactivation of the enzyme by the reaction products. as shown by electrospray mass spectrometry, the inactivation of cpha by cefoxitin and moxalactam is accompanied by the formation of stable adducts with mass increases of 445 and 111 da, respectively. the single thiol group of the inactivated enzyme is no longer titrable, and ...200111432752
case report: recovery of calliphora vicina first-instar larvae from a human traumatic wound associated with a progressive necrotizing bacterial infection.human myiasis caused by calliphora vicina is rare in europe. here we report a case of c. vicina infection occurring in the traumatic leg wound of a healthy 21-year-old man. firstly, a progressive necrotizing infection developed in the wound despite administration of antibiotics. aeromonas hydrophila was isolated from the wound samples. secondly, during debridement, c. vicina first-instar larvae were isolated from the wound. to our knowledge, this is the first european case of c. vicina wound myi ...200111442211
resistance to antibiotics and biocides among non-fermenting gram-negative bacteria.to investigate the antibiotic and biocide susceptibilities of clinical isolates of rarely encountered gram-negative, non-fermenting bacteria.200111442564
[lipids of microorganisms from the family vibrionaceae causing diseases in fishes].lipid fractional and fatty acid compositions of microorganisms from the genera aeromonas, pseudomonas, and vibrio (the family vibrionaceae), causing diseases of different fish species, were studied. motile aeromonads and vibrios displayed higher relative contents of membrane lipids and oleic acid and lower relative contents of storage lipids compared with immotile aeromonads and pseudomonads, which is connected with the activities of their movements. immotile aeromonads and vibrios exhibited hig ...200111443908
prostacyclin is neither sufficient alone nor necessary to cause pulmonary dysfunction: results from infusions of prostacyclin and antiprostacyclin antibody in porcine septic shock.this study evaluated whether prostacyclin is a necessary mediator of inflammation in graded bacteremia or is sufficient alone in pathophysiologic concentrations to cause the pulmonary derangement of bacteremic shock.200111445706
production of n-acyl homoserine lactones by gram-negative bacteria isolated from contact lens wearers.the purpose of this study was to investigate the production of n-acyl-homoserine lactone (ahl) signal molecules in ocular gram-negative bacteria. a total of 91 ocular strains isolated from contact lens adverse response patients and asymptomatic subjects were used in the study. these included acinetobacter, aeromonas hydrophila, escherichia coli, haemophilus influenzae, klebsiella oxytoca, pseudomonas aeruginosa, serratia liquefaciens, serratia marcescens, and stenotrophomonas maltophilia. the bi ...200111446456
simulating the effect of eliminating routine bacterial screening on the negative predictive value of the ontario fish hatchery disease monitoring program.we determined the impact of eliminating routine screening for aeromonas salmonicida and yersinia ruckeri on the efficacy of the ontario ministry of natural resources (omnr) fish disease monitoring program, using monte carlo simulation. because the main purpose of the program is to prevent transferring infected fish among omnr hatcheries, or to wild fish populations through stocking waterways, the hatchery-level negative predictive value (hnpv) was used as an indicator of monitoring efficacy. the ...200111448498
identification of a plasmid encoding shv-12, tem-1, and a variant of imp-2 metallo-beta-lactamase, imp-8, from a clinical isolate of klebsiella pneumoniae.a multidrug-resistant plasmid encoding tem-1, shv-12, and a variant of imp-2 metallo-beta-lactamase, designated imp-8, was identified from a clinical isolate of klebsiella pneumoniae. there are four nucleotide differences between bla(imp-2) and bla(imp-8), resulting in two amino acid differences. bla(imp-8) was also found to be carried by an integron-borne gene cassette similar to the bla(imp-2) cassette.200111451699
[the effect of tannery waste products on viability of selected bacteria from activated sludge].in this study we have investigated the influence of sludge on the activity of bacteria strains: acinetobacter baumannii, aeromonas hydrophila and pseudomonas cepacia. to our studies we have taken waste materials used in the chromium compound leather tanning process as well as materials from leather fat and waste materials, in general. it was confirmed that 1:20 dilution of chromium tanning leather waste, retanage and leather fat allowed the survivability of bacteria in all three strains. through ...200111452739
tract microflora in saudi patients with cholelithiasis.to identify the microflora in the gallbladder of patients undergoing laparoscopic cholecystectomy for gallstones, and the antibiotic susceptibility pattern of the isolates, as well as the usefulness of gram staining of bile at the time of operation.200111469952
infectious complications of cirrhosis.infectious complications in cirrhotic patients can cause severe morbidity and mortality. bacterial infections are estimated to cause up to 25% of deaths in cirrhotic patients. the most frequent are urinary tract infection, spontaneous bacterial peritonitis, respiratory tract infection, and bacteremia. it has been said that cirrhosis is the most common form of acquired immunodeficiency, exceeding even aids. the specific risk factors for infection in cirrhotic patients are low serum albumin, gastr ...200111469997
immunoglobulin genes and antibody responses in the spotted wolffish (anarhichas minor olafsen).the spotted wolffish anarhichas minor olafsen is a promising new species in aquaculture in the cold waters of northern norway. in this paper, some basic immunological studies of this marine species are reported. of comparative interest are the cdna sequences of the immunoglobulin transcript and the antibody responses to model antigens. of more practical importance are the humoral immune responses and antibody specificities to potentially pathogenic bacteria. full length cdna clones encoding the ...200111478516
interactions of streptomyces griseus aminopeptidase with amino acid reaction products and their implications toward a catalytic mechanism.streptomyces griseus aminopeptidase (sgap) is a double-zinc exopeptidase with a high preference toward large hydrophobic amino-terminus residues. it is a monomer of a relatively low molecular weight (30 kda), it is heat stable, it displays a high and efficient catalytic turnover, and its activity is modulated by calcium ions. the small size, high activity, and heat stability make sgap a very attractive enzyme for various biotechnological applications, among which is the processing of recombinant ...200111484227
bacterial causes of aids-associated diarrhea in thailand.the incidence of bacterial diarrhea in aids patients has increased steadily and has led to enormous medical and public health problems. in this study, the clinical data together with 350 rectal swab samples each from aids patients with diarrhea (apd) and non-aids patients with diarrhea (napd), were collected and examined for bacterial enteropathogens at the bamrasnaradura infectious diseases hospital (bidh), nonthaburi, thailand from may to december 1996. patients were matched by age and sex. th ...200111485080
[determination of aeromonas spp. and pseudomonas spp. in chlorinated water supply]. 200111490893
enzymatic activity of micro-organisms isolated from cork wine stoppers.the production of enzymes by micro-organisms which are found on vegetal substrates is important due to their ability to decompose cellulose, lignin and other components, which guarantee the integrity of the vegetal cell. the objective of this study was to determine the enzymatic activity of filamentous fungi, yeasts and bacteria, isolated from natural cork stoppers for bottles of still and sparkling wines. suspensions of fungal conidia, yeasts and bacterial cells of micro-organisms were establis ...200111491526
[maturation pathway of hemolysin of aeromonas sobria and the mechanism of action of the hemolysin].aeromonas sobria has been recognized as pathogens associated with acute gastroenteritis in both adults and children. the major virulence factor has been proposed to be hemolysin which possesses both hemolytic and enterotoxic activities. mature (bio-active) hemolysin secreted out of cells binds to the target cells of the host and injure the cells. however, hemolysin remained in bacteria can not express such toxicity. it means that the maturation and secretion pathway of hemolysin is closely relat ...200111494595
virulence factors in vibrios and aeromonads isolated from seafood.thirty-one isolates from seafood, identified as aeromonas hydrophila (7), aeromonas caviae (11), vibrio parahaemolyticus (3), vibrio fluvialis (5), vibrio alginolytictus (3), vibrio metschnikovii (1) and vibrio damsela (1), were tested for possible virulence factors including extracellular hydrolytic enzymes, haemolysins, cytotoxins (vero and hep-2 cells) and adherence ability (hep-2 cells). all the a. hydrophila strains were beta-haemolytic and produced cytotoxins as well as one strain of v. fl ...200111497085
induction of inflammatory cytokines by extracellular products and lps of the fish pathogen aeromonas salmonicida ssp. achromogenes in mice and mouse cell cultures.balb/c mice, injected i.p. with extracellular products (ecp) of aeromonas salmonicida subsp. achromogenes (asa), displayed symptoms similar to toxic shock syndrome. the ld(50) observed was between 1.5 and 2.0 microg g(-1) and the mice died within 19 h. four inflammatory cytokines were measured in mice receiving sublethal ecp doses. tnf-alpha and il-6 showed a sharp peak in the serum while il-1 beta and il-2 were not detected. when peritoneal macrophages were cultivated in the presence of ecp, as ...200111498248
rapid iodometric detection of aeromonas amylase and its diagnostic significance.twenty-five aeromonas hydrophila isolates from different sources (food, 13; clinical, 6 and environmental, 6) were studied for the mode of production of amylase and rapid iodometric detection of the enzyme in vitro. all twenty-five of the isolates produced the enzyme constitutively at 37 degrees c. amylase producing ability was not dependent on the source of isolation of aeromonas (f = 0.1069; p > 0.05). using iodometric technique, in a microtitration tray, the enzyme was fully demonstrated in 1 ...200111502374
microbiological quality of the bivalve pinctada imbricata commercialized in cumaná, venezuela.the habitual consumption of oysters in the city of cumaná, venezuela, is made in poor sanitary conditions, therefore a study was carried out to determine the sanitary quality of the product. for this, 120 samples of oyster pinctada imbricata were collected directly from: a) fishing boats (unhandled samples) and b) after being shucked by the salesman (handled samples). unhandled samples showed the following mpn/g: faecal coliforms < 3 - 2.2 x 10, aeromonas sp. < 3 - 1.1 x 10(3), staphylococcus au ...200111510429
heat resistance and growth of salmonella enteritidis, listeria monocytogenes and aeromonas hydrophila in whole liquid egg.salmonella enteritidis atcc,13067, listeria monocytogenes atcc,19116 and aeromonas hydrophila atcc,7965 strains were evaluated for growth and thermal resistance in liquid whole egg (lwe). each strain grew well in lwe at temperatures between 4 and 30 degrees c, except s. enteritidis which grew weakly at 4 and 10 degrees c. maximum populations for each strain increased with increasing growth temperature. the thermal destruction of each strain was determined in six liquid products. the egg products ...200111518391
typing of aeromonas isolates from children with diarrhoea & water samples by randomly amplified polymorphic dna polymerase chain reaction & whole cell protein fingerprinting.aeromonas spp. are water-borne organisms, often associated with childhood diarrhoea. the present study was conducted to examine the epidemiological relationship among the aeromonas spp. isolated from water and children with acute diarrhoea in chennai.200111525157
a secreted aminopeptidase of pseudomonas aeruginosa. identification, primary structure, and relationship to other aminopeptidases.using leucine-p-nitroanilide (leu-pna) as a substrate, we demonstrated aminopeptidase activity in the culture filtrates of several pseudomonas aeruginosa strains. the aminopeptidase was partially purified by deae-cellulose chromatography and found to be heat stable. the apparent molecular mass of the enzyme was approximately 56 kda; hence, it was designated ap(56). heating (70 degrees c) of the partially purified aminopeptidase preparations led to the conversion of ap(56) to a approximately 28-k ...200111533066
antibody response in salmonids against the 70 kda serine protease of aeromonas salmonicida studied by a monoclonal antibody-based elisa.an antigen-capture enzyme-linked immunosorbent assay (celisa) based on monoclonal antibodies (mab) was set up and evaluated for selective detection of salmonid antibody responses to the antigen p1, which is a weakly immunogenic exoprotease of typical aeromonas salmonicida. this new assay permits a specific determination of anti-protease-antibodies, without antigen purification. serum antibodies induced by the strongly immunogenic lipopolysaccharide could reliably be discriminated from anti-p1-an ...200111557299
a randomized, blinded, controlled trial investigating the gastrointestinal health effects of drinking water quality.a double-blinded, randomized, controlled trial was carried out in in melbourne, australia, to determine the contribution of drinking water to gastroenteritis. melbourne is one of the few major cities in the world that draws drinking water from a protected forest catchment with minimal water treatment (chlorination only). six hundred families were randomly allocated to receive either real or sham water treatment units (wtus) installed in their kitchen. real units were designed to remove viruses, ...200111564611
enteropathogenicity of plesiomonas shigelloides and aeromonas spp. in experimental mono- and coinfection with cryptosporidium parvum in the intestine of neonatal balb/c mice.enteropathogenicity of plesiomonas shigelloides, aeromonas hydrophila, a. caviae and a. sobria was studied both in monoinfections and in coinfections with coccidium cryptosporidium parvum in neonatal balb/c mice. in monoinfection experiments, neonatal balb/c mice were orally infected with 7 x 10(7) or 7 x 10(8) cfu, respectively, of a strain of p. shigelloides or a strain of an aeromonas spp. in coinfection experiments, the neonatal mice were, in addition to being orally infected with one of the ...200111131040
crystallization and preliminary x-ray analysis of (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.dimeric (r)-specific enoyl-coenzyme a (coa) hydratase from aeromonas caviae catalyzes the hydration of trans-2-enoyl-coas with carbon lengths of 4-6 to yield their corresponding (r)-3-hydroxyacyl-coas and is essential for polyhydroxyalkanoate (pha) biosynthesis. the enzyme has been crystallized by vapour diffusion against a reservoir solution containing 20% polyethylene glycol 4000, 5% 2-propanol and 20 mm hepes ph 7.0 at 298 k. crystals belong to the monoclinic space group c2, with unit-cell pa ...200111134939
locomotion and feeding of acanthamoeba at the water-air interface of ponds.acanthamoeba trophozoites attach to and effect amoeboid locomotion at the water-air interface of ponds. their locomotory rate (approximately 0.8 microm s(-1)) and manner of independent movement at this interface is similar to that over solid substrata. adhesion forces developed between amoebae and the water-air interface are greater than gravity and thus amoebae are also transported passively without detachment. amoebae docked with the water-air interface remain and flourish here as they are sho ...200111164298
miniaturized tests for computer-assisted identification of motile aeromonas species with an improved probability matrix.to develop miniaturized tests for the phenotypic identification of motile aeromonas species using an improved probability matrix.200111168721
detection of vibrionaceae in mussels and in their seawater growing area.the seasonal trend and frequency of detection of vibrionaceae in seawater samples and in molluscs collected in the adriatic sea was measured.200111169043
intestinal secretory immunoglobulin a (siga) response to aeromonas exoproteins in patients with naturally acquired aeromonas diarrhea.the secretory immunoglobulin a (siga) response at the intestinal mucosa is one of the primary defense mechanisms protecting against enteric infections and may therefore be used as an indicator of bacterial enteropathogenicity. in order to understand the role played by aeromonas strains as gastrointestinal infectious agents, the siga response in fecal specimens obtained from patients with naturally acquired aeromonas diarrhea was examined. our results demonstrated a specific siga response which w ...200111172988
isolation and identification of bacteria associated with adult laboratory mexican fruit flies, anastrepha ludens (diptera: tephritidae).from the guts of new and old colonies (female and male) of mexican fruit flies, anastrepha ludens (diptera: tephritidae), we identified a total of 18 different bacterial species belonging to the family enterobacteriaceae, pseudomonadaceae, vibrionaceae, micrococcaceae, deinococcacea, bacillaceae, and the genus listeria. enterobacter, providencia, serratia, and staphylococcus spp. were the most frequently isolated genera, with citrobacter, streptococcus, aerococcus, and listeria found less freque ...200111178731
cell vacuolation caused by vibrio cholerae hemolysin.non-o1 strains of vibrio cholerae implicated in gastroenteritis and diarrhea generally lack virulence determinants such as cholera toxin that are characteristic of epidemic strains; the factors that contribute to their virulence are not understood. here we report that at least one-third of diarrhea-associated nonepidemic v. cholerae strains from mexico cause vacuolation of cultured vero cells. detailed analyses indicated that this vacuolation was related to that caused by aerolysin, a pore-formi ...200111179335
metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum.eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ...200111181369
the occurrence of aeromonas spp. in natural mineral water and well water.the presence of motile aeromonas spp. in natural mineral water (nmw) and drinking well water in the area of lecce (italy) was investigated. aeromonas spp. were not detected in any of the 60 nmw samples either by the direct and enrichment method. from a total of 20 wells, five were found to contain aeromonas species with cell number ranging from 26 to 1609 250 ml(-1). in two wells the presence of aeromonas spp. was not associated to the presence of faecal indicators, i.e. coliforms and faecal col ...200111205949
in vitro and in vivo combinations of cefotaxime and minocycline against aeromonas hydrophila.the activities of cefotaxime and minocycline against aeromonas hydrophila were investigated. cefotaxime (4 times the mic) plus minocycline (0.75 times the mic) elicited an inhibitory effect for 48 h in a time-kill study, and more infected mice treated with both drugs survived (91%) than survived after treatment with cefotaxime (9%) or minocycline (44%) alone, suggesting that cefotaxime and minocycline act synergistically against a. hydrophila.200111257047
direct screening of the imp-1 metallo-beta-lactamase gene (blaimp) from urine samples by polymerase chain reaction.as gram-negative bacterial isolates producing plasmid-mediated imp-1 metallo-beta-lactamase usually demonstrate resistance to various broad-spectrum beta-lactams, including cephamycins and carbapenems, transmission and proliferation of these microorganisms in clinical settings could become a clinical threat in the near future. according to previous studies by the same authors, imp-1-producing strains are usually isolated from urine samples. therefore, in this study, a polymerase chain reaction ( ...200111260335
bacteriological and virological quality of seawater bathing areas along the tyrrhenian coast.monitoring was carried out during summer 1997 along a selected area of the tyrrhenian coast near the tiber river mouth. fifty-eight seawater samples, collected from 19 stations, were examined for coliforms, streptococci, enteroviruses, salmonellae, coliphages, bacteroides fragilis phages, pseudomonas, alophilic vibrios, aeromonas and yeasts. salmonellae and coliphages were isolated in 3 and 12 out of 58 samples, respectively. enteroviruses and bacteroides fragilis phages were not isolated. reovi ...200111260783
antibacterial effects of different food-related phosphates using aeromonas hydrophila.aeromonas hydrophila is considered to be an emergent food-related bacterium. phosphates are used as additives, mainly in meat products, to improve the quality of these foods. the antibacterial properties of phosphates are also well known. in this work, two a. hydrophila strains in early exponential phase were used: (a) a. hydrophila atcc 7965 and (b) a. hydrophila derived from food, isolated in our laboratory. mic and mbc studies were performed to assess the antibacterial effects of four phospha ...200111271767
biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates.one hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. analysis of biodiversity at the species level was performed by comparing the alui restriction patterns of the 16s ribosomal dna amplified by pcr from 120 isolates. the rdna restriction analysis enabled us to c ...200111281319
prevalence, species differentiation, haemolytic activity, and antibiotic susceptibility of aeromonads in untreated well water.the use of untreated water for drinking and other activities have been associated with intestinal and extraintestinal infections in humans due to aeromonas species. in the present study aeromonads were isolated from 48.7% of 1,000 water samples obtained from wells and other miscellaneous sources. aeromonas species were detected in 45% of samples tested in spring, 34.5% in summer, 48% in autumn and 60% of samples tested in winter. speciation of 382 strains resulted in 225 (59%) being a. hydrophil ...200111285492
enhancement of the virulence of aeromonas caviae diarrhoeal strains by serial passages in mice.thirteen clinical aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (ctc) factor production, by four consecutive passages through mice by intraperitoneal injection of a. caviae suspensions. the passaged a. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. all a. caviae isolates pos ...200111289515
identification of a 43-kda outer-membrane protein as an adhesin in aeromonas caviae.aeromonas spp. are associated with intestinal and extra-intestinal infections. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interactions involved in the adherence of a. caviae isolates ae56, ae391 and ae398 to hep-2 cells. all strains expressed high levels of aggregative adherence. maximum adhesion occurred with bacteria grown at 22 degrees c, but transmission electron microscopy did not reveal the presence of fimbrial structures on ...200111289516
endocarditis due to rare and fastidious bacteria.the etiologic diagnosis of infective endocarditis is easily made in the presence of continuous bacteremia with gram-positive cocci. however, the blood culture may contain a bacterium rarely associated with endocarditis, such as lactobacillus spp., klebsiella spp., or nontoxigenic corynebacterium, salmonella, gemella, campylobacter, aeromonas, yersinia, nocardia, pasteurella, listeria, or erysipelothrix spp., that requires further investigation to establish the relationship with endocarditis, or ...200111148009
hatchery-level predictive values for infectious pancreatic necrosis virus and aeromonas salmonicida in ontario, canada.the probability and uncertainty of correctly classifying the ipnv and aeromonas salmonicida status of fish-rearing and natural sites in ontario were estimated through monte carlo simulations. propagating several uncertain inputs showed the extent to which natural variability and our present lack of knowledge affect the probability of site misclassification. for the scenarios investigated, the site-level negative predictive values (snpvs) were high and fairly constant. the site-level positive pre ...200111154785
microbial growth modelling with artificial neural networks.there is a growing interest in modelling microbial growth as an alternative to time-consuming, traditional, microbiological enumeration techniques. several statistical models have been reported to describe the growth of different microorganisms, but there are accuracy problems. an alternate technique 'artificial neural networks' (ann) for modelling microbial growth is explained and evaluated. published data were used to build separate general regression neural network (grnn) structures for model ...200111294356
association between major histocompatibility complex class iib alleles and resistance to aeromonas salmonicida in atlantic salmon.we have tested the importance of genetic variation in the major histocompatibility complex (mhc) class iib in atlantic salmon (salmo salar) for survival after challenge with a highly virulent bacterial pathogen. forty juvenile full siblings from each of 120 families were infected with the bacterium aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. fishes from high-resistance (hr, < 35% mortality) and low-resistance (l,r, > 80% mortality) families were screened for ...200111296859
development of a latex agglutination test for rapid identification of escherichia coli.escherichia coli, one of the most important human pathogens, is usually identified by a battery of biochemical tests that require overnight incubation. for rapid identification of escherichia coli, a latex agglutination test (lat) was developed. rabbits were immunized with cell-surface antigens extracted from escherichia coli ccrc 15481 with 4 m urea, and the affinity-purified antibodies were used to coat latex particles for the identification of the bacterium. the following gram-negative bacter ...200111305479
roles of an endogenous serum lectin in the immune protection of blue gourami, trichogaster trichopterus (pallus) against aeromonas hydrophila.the serum of blue gourami, trichogaster trichopterus (pallus), contains a calcium-dependent, n-acetyl-galactosamine-binding lectin (bgl) which efficiently activates and enhances the non-specific immune response of fish towards a virulent strain of aeromonas hydrophila. in the in vitro studies, a lectin concentration range of 0.05-1.0 ng ml(-1) was found to significantly promote phagocytic uptake of the bacteria by macrophages. this effect was further augmented when purified lectin was combined w ...200111308073
mucosal immune response of spotted sand bass paralabrax maculatofasciatus (steindachner, 1868) orally immunised with an extracellular lectin of aeromonas veronii.to assess the immunogenic and immunoprotective role of the extracellular lectin from aeromonas veronii (mcbp), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (paralabrax maculatofasciatus) were orally immunised either with soluble mcbp, adjuvant-conjugated mcbp or immobilised mcbp on latex microspheres. the results suggest that the mcbp is capable of eliciting protective immunity against a. veronii infections when administe ...200111308074
a monoclonal antibody recognising a surface marker on rainbow trout (oncorhynchus mykiss) monocytes.the characterisation of a monoclonal antibody (mab 45) reacting with phagocytic leucocytes isolated from blood and spleen of rainbow trout (oncorhynchus mykiss, walbaum) is described. the surface marker labelled by this mab is expressed at relative low levels on the membrane of large, nearly nongranulated trout leucocytes, and having the typical morphology of monocytes in flow cytometry (kfoury et al., 1999, fish pathology, 34, 1-6). no reaction of mab 45 with granulocytes, lymphocytes or thromb ...200111308075
characterisation of the culturable heterotrophic bacterial community in a small eutrophic lake (priest pot).the community composition and structure of planktonic heterotrophic bacteria (903 isolates) sampled from a small eutrophic lake in northern england (priest pot) was studied with respect to season (four samples) and depth (to 3.1 m). bacteria (887) were isolated on tryptic soy broth agar and identified to 48 genera using fatty acid methyl ester analysis. the two most abundant genera isolated were aeromonas and pseudomonas which, respectively, dominated the middle to bottom depths in august and al ...200111311440
host factors associated with the detection of aeromonas salmonicida and yersinia ruckeri in ontario, canada government fish hatcheries.this paper presents an epidemiological investigation of ontario ministry of natural resources fish health laboratory data from 1981 to 1997, to determine whether fish species and age were associated with lot-level detection of aeromonas salmonicida and yersinia ruckeri in hatchery fish. in stepwise logistic regression, the species brook trout and back-cross (lake trout crossed with the hybrid "splake") were more likely to test a. salmonicida-positive compared to all other species reared in the h ...200111311951
contamination of potable roof-collected rainwater in auckland, new zealand.one-hundred and twenty-five domestic roof-collected rainwater supplies in four rural auckland districts were investigated in a cross-sectional survey to determine water quality. samples of cold faucet water were analysed for physico-chemical and microbiological determinands, including metals (zinc, copper and lead), bacterial indicator organisms--heterotrophic plate count (hpc), total coilforms (tc), faecal coliforms (fc), enterococci (ent), bacterial pathogens including salmonella spp., legione ...200111317899
histopathology of viremia-associated ana-aki-byo in combination with aeromonas hydrophila in color carp cyprinus carpio in japan.a disease in which 'viremia-associated ana-aki-byo' is combined with an aeromonas hydrophila infection currently occurs and is highly transmissible in color carp cyprinus carpio in japan. in the present study, to determine the interrelation between a corona-like virus and a. hydrophila, we conducted transmission trials by cohabiting naturally diseased carp with healthy carp with skin that had been slightly damaged artificially. experimentally exposed fish successfully replicated the combination ...200111324811
cloning, expression, and characterization of a family 52 beta-xylosidase gene (xysb) of a multiple-xylanase-producing bacterium, aeromonas caviae me-1.a lambda phage genomic library of aeromonas caviae me-1, a multiple-xylanase-producing bacterium, was screened for xylan degradation activities. we isolated one clone, b65, which had weak xylanase activity, by the dns method, but gave no visible bands on zymogram assay using sds-xylan-page. based on tlc analyses of enzymatic products and some glycosidase assays using p-nitrophenyl substrates, we established that pb65 encodes a beta-xylosidase gene. in the nucleotide sequence analysis, we found a ...200111330658
[aeromonas spp bacteremia: study of 12 cases and review of the literature].twelve cases of aeromonas spp. bacteremia are here reviewed in adult patients occurred at our institution during a 6-year period. three cases corresponded to patients with hematological disease and four had a solid neoplasm. the source of infection in seven patients was extra-nosocomial; infections in the five remaining patients were considered to be acquired in the hospital. in seven patients, potential portals of entry were found. the usual clinical presentation was febrile syndrome in all cas ...200111333602
cellulitis and septicemia caused by aeromonas hydrophila acquired at home. 200111339474
a case of spontaneous bacterial empyema and bacteremia caused by aeromonas hydrophila. 200111347676
mesophilic aeromonads in wild and aquacultured freshwater fish.numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ...200111348001
the occurrence of aerolysin-positive aeromonas spp. and their cytotoxicity in norwegian water sources.the aim of the study was to investigate the occurrence of aeromonas spp. and their aerolysin status in norwegian natural water sources.200111348441
escherichia coli crebc is a global regulator of gene expression that responds to growth in minimal media.we have identified nine genes, the expression of which are regulated by the crebc two-component system: the first members of the cre regulon. they are divided into eight transcriptional units, each having a promoter-proximal ttcacnnnnnnttcac "cre-tag" motif. the cre regulon genes are: the acka/pta operon, the products of which collectively catalyze the conversion of acetyl-coa into acetate and atp; tala, which encodes an enzyme involved in the mobilization of glyceraldehyde-3-phosphate into the ...200111350954
biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered escherichia coli strains.the recombinant escherichia coli strain, equipped with the newly cloned aeromonas pha biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3hb), 3-hydroxyvalerate (3hv), and 3-hydroxyhexanoate (3hhx) [p(3hb-co-3hv-co-3hhx)] from dodecanoic acid plus odd carbon number fatty acid. in addition, the orf1 gene of aeromonas hydrophila was found to play a critical role in assimilating the 3hv monomer and in regulating the monomer fraction in the terpolymer.200111353413
centa as a chromogenic substrate for studying beta-lactamases.centa, a chromogenic cephalosporin, is readily hydrolyzed by beta-lactamases of all classes except for the aeromonas hydrophila metalloenzyme. although it cannot practically be used for the detection of beta-lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.200111353639
g561 site-directed deletion mutant chitinase from aeromonas caviae is active without its 304 c-terminal amino acid residues.a g561 mutant of the aeromonas caviae chitinase chia was made by pcr site-directed deletion mutagenesis in order to study the role of the 304 c-terminal amino acid residues of chia in the enzymatic hydrolysis of chitin. the recombinant chiag561 encoded on a 1.6-kb dna fragment of a. caviae chia was expressed in a heterologous escherichia coli host using the pet20b(+) expression system. the his-tag-affinity-purified recombinant chiag561 had a calculated molecular mass of 63,595 da, which was cons ...200111357514
characterization of the pro-aminopeptidase from aeromonas caviae t-64.the pro-aminopeptidase from aeromonas caviae t-64 (pro-apac) had maximal activity at 60 degrees c and was more stable than mature apac at temperature up to 65 degrees c for 1 hour. the ph stability of pro-apac ranged from 4.0 to 8.0, which is broader than the range for the mature apac. the kcat/km of pro-apac was 1.4% to 24% of that of mature apac.200111302179
synergy of histone-derived peptides of coho salmon with lysozyme and flounder pleurocidin.recent research has identified endogenous cationic antimicrobial peptides as important factors in the innate immunity of many organisms, including fish. it is known that antimicrobial activity, as well as lysozyme activity, can be induced in coho salmon (oncorhynchus kisutch) mucus after exposure of the fish to infectious agents. since lysozyme alone does not have antimicrobial activity against vibrio anguillarum and aeromonas salmonicida, a four-step protein purification protocol was used to is ...200111302792
plasmid-encoded metallo-beta-lactamase (imp-6) conferring resistance to carbapenems, especially meropenem.in 1996, serratia marcescens ku3838 was isolated from the urine of a patient with a urinary tract infection at a hospital in northern japan and was found to contain the plasmid pku501. previously, we determined that pku501 carries bla(imp) and the genes for tem-1-type beta-lactamases as well as producing both types of beta-lactamases (h. yano, a. kuga, k. irinoda, r. okamoto, t. kobayashi, and m. inoue, j. antibiot. 52:1135-1139, 1999). pku502 is a recombinant plasmid that contains a 1.5-kb dna ...200111302793
[metallo-beta-lactamase producing bacteria].metallo-beta-lactamases are the carbapenemases belonging to the class b beta-lactamases on a molecular level. two types of the metallo-beta-lactamase, imp-1 and vim-1, which have been detected mainly from pseudomonas aeruginosa, respectively, in japan and in europe are especially important, because of the broad substrate specificity. these enzymes confer resistance to not only the carbapenems but also almost all beta-lactam antibiotics expect for monobactams. clinical strains producing imp-1 typ ...200111304992
formation of viable but non-culturable state (vbnc) of aeromonas hydrophila and its virulence in goldfish, carassius auratus.in this study we investigated the viable but non-culturable (vbnc) state of aeromonas hydrophila and its virulence in goldfish. aeromonas hydrophila cultured in a 0.35% nacl solution at ph 7.5 and at 25 degrees c for 50 days showed the vbnc state. in the vbnc state we were unable to detect viable bacteria by the plate count method but we did find 10(4) cells/ml by the direct viable count microscopical method after staining with fluorescein diacetate and ethidium bromide. the virulence comparison ...200111372646
characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish.the present study aimed to investigate the potential probiotic properties of six lactic acid bacteria (lab) intended for human use, lactobacillus rhamnosus atcc 53103, lactobacillus casei shirota, lactobacillus bulgaricus, l. rhamnosus lc 705, bifidobacterium lactis bb12, and lactobacillus johnsonii la1, and one for animal use, enterococcus faecium tehobak, for use as a fish probiotic. the strains for human use were specifically chosen since they are known to be safe for human use, which is of m ...200111375147
16s rrna in situ probing for the determination of the family level community structure implicated in enhanced biological nutrient removal.knowledge of a discrete physiological group capable of excess biological phosphate removal (ebpr) remains unclear. consequently, microbial community analysis of an enhanced continuous laboratory-scale activated sludge process displaying a strong ebpr mechanism was conducted. unit design was configured upon the three-stage phoredox process and characterization of the activated sludge bacterial community was carried out using fluorescent in situ hybridization (fish) techniques. fixed activated slu ...200111379117
clostridium perfringens as a potential indicator for the presence of sewage solids in marine sediments.marine sediment cores collected from several depths of water and distances from a california sewage outfall were tested to see if sediments influenced by sewage solids were a reservoir of enteric pathogens, and if concentrations of indicator bacteria were related to the presence of sewage solids. vertical distributions of microorganisms in marine sediments were determined; there was a decrease of indicator bacteria with increasing sediment depth. aeromonas was randomly isolated, but none of the ...200111382981
characterization of class 1 integrons associated with r-plasmids in clinical aeromonas salmonicida isolates from various geographical areas.class 1 integrons were found in 26 of 40 antibiotic-resistant isolates of the fish pathogen aeromonas salmonicida from northern europe and north america. three different dhfr genes, conferring trimethoprim resistance, and one ant(3")1a aminoglycoside resistance gene were identified as gene inserts. the gene cassettes tended to be conserved among isolates from a particular geographical area. nineteen isolates transferred r-plasmids carrying different tet determinants to escherichia coli in filter ...200111389105
conserved anchoring mechanisms between crystalline cell surface s-layer proteins and secondary cell wall polymers in gram-positive bacteria? 200111173224
motility and the polar flagellum are required for aeromonas caviae adherence to hep-2 cells.aeromonas caviae is increasingly being recognized as a cause of gastroenteritis, especially among the young. the adherence of aeromonads to human epithelial cells in vitro has been correlated with enteropathogenicity, but the mechanism is far from well understood. initial investigations demonstrated that adherence of a. caviae to hep-2 cells was significantly reduced by either pretreating bacterial cells with an antipolar flagellin antibody or by pretreating hep-2 cells with partially purified f ...200111401962
comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in belgium.the aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxytetracycline-resistant (ot(r)) heterotrophic bacterial population in two belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. the study of ot(r) heterotrophs and mesophilic aeromonas spp. allowed comparison of tolerance data at the intergenus as well as at the intragenus level. in total, 354 independently obtained ot(r) is ...200111403391
[beta-lactam antibiotic sensitivity in aeromonas spp. of clinical, animal, and environmental origin].susceptibility to beta-lactam antibiotics was investigated in aeromonas spp. microorganisms were isolated from both, clinical and water creek samples, as well as from processed raw chicken carcasses. aeromonas like colonies were identified by means of aerokey ii and api 20 e system (bio-merieux). a. hydrophila prevailed both of human origin (44%) and water creek samples (41%), while a. caviae ranked first among raw chicken samples (65%). dilution testing by agar method was performed to determine ...200111407020
effects of bacteria on the growth of an amoeba infecting the gills of turbot.we analysed the influence of various bacteria on the in vitro growth of trophozoites of a platyamoeba strain isolated from diseased gill tissues of cultured turbot. little or no growth was shown by amoebae cultured in the presence of (1) the turbot-pathogenic bacteria vibrio anguillarum, aeromonas salmonicida or streptococcus sp., (2) pasteurella piscicida or vibrio vulnificus (pathogenic for some fishes but not turbot), or (3) the non-pathogenic 'environmental' bacteria vibrio campbelli, vibrio ...200111411647
characterization of aeromonas and vibrio species isolated from a drinking water reservoir.to study the phenotypic and chemotaxonomic (i.e. phospholipid and cellular fatty acid composition) characteristics of environmental aeromonas spp. and vibrio spp. isolated from a drinking water reservoir near vladivostok city, and the application of some chemotaxonomic markers for discrimination of the two genera and species.200111412322
the channel-forming protein proaerolysin remains a dimer at low concentrations in solution.proaerolysin, the proform of the channel-forming protein aerolysin, is secreted as a dimer by aeromonas sp. the protein also exists as a dimer in the crystal, as well as in solution, at least at concentrations in the region of 500 microg/ml. recently it has been argued that proaerolysin becomes monomeric at concentrations below 100 microg/ml and that only the monomeric form of the protoxin can bind to cell surface receptors (fivaz, m., velluz, m.-c., and van der goot, f. g. (1999) j. biol. chem. ...200111016941
immune responses of nile tilapia (oreochromis niloticus l.) clones: i. non-specific responses.the importance of genetic variation in the non-specific immune responses of nile tilapia (oreochromis niloticus l.) clones was investigated. fully inbred clones (ic) of nile tilapia, produced using gynogenesis and sex reversal, and crosses between these lines (outbred clones) were used in this study. non-specific immune responses were compared between the ics, including serum lysozyme activity and phagocytosis, and significant differences were observed between the different groups. their natural ...200110980318
phylogeny of the major head and tail genes of the wide-ranging t4-type bacteriophages.we examined a number of bacteriophages with t4-type morphology that propagate in different genera of enterobacteria, aeromonas, burkholderia, and vibrio. most of these phages had a prolate icosahedral head, a contractile tail, and a genome size that was similar to that of t4. a few of them had more elongated heads and larger genomes. all these phages are phylogenetically related, since they each had sequences homologous to the capsid gene (gene 23), tail sheath gene (gene 18), and tail tube gene ...200111114936
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