TitleAbstractYear(sorted ascending)
rapid purification of the aeromonas proteolytica aminopeptidase: crystallization and preliminary x-ray data.the heat-stable aminopeptidase from aeromonas proteolytica has been purified using two new procedures, with the aim of preparing large single crystals for x-ray analysis. in a first procedure, we tried to avoid any drastic conditions capable of inducing microheterogeneities in the protein sample. the enzyme was purified through two chromatographic steps based on hydrophobic interactions and ion exchange. in a second procedure a heat treatment of the protein to a temperature of 70 degrees c over ...19921550363
myonecrosis due to aeromonas hydrophila following insertion of an intravenous cannula: case report and review. 19921554856
isolation of salmonellas by immunomagnetic separation.magnetisable particles, coated with anti-salmonella serum, were used to isolate salmonella livingstone from pure cultures, mixed cultures and food samples. beads (10(7] were generally incubated with 10(4) salm. livingstone cells/ml for 60 min at room temperature. the incubation and washing medium (0.01 mol/l phosphate-buffered saline; pbs) contained 0.1% bovine serum albumin (bsa) and 0.1% tween 20, respectively. this method gave a recovery for salm. livingstone of 51.0 +/- 7.8%. however, other ...19921556036
enterotoxicity of clinical and environmental isolates of aeromonas spp.of 147 isolates of three species of aeromonas, 54 were from clinical and 93 from environmental sources. when tested for enterotoxin production, most of the isolates (56%) caused accumulation of fluid in rabbit ileal loops (rils). although large proportions of clinical and environmental isolates of a. caviae (55% and 65%, respectively) elicited such a response in rils, isolates of a. hydrophila and a. sobria produced significantly more fluid (p less than 0.05). furthermore, the environmental stra ...19921560448
catfish-related injury and infection: report of two cases and review of the literature.two cases of serious infection following catfish spine-related injuries are presented, and the literature on this topic is reviewed. the organisms usually involved in such infections are vibrio species, aeromonas hydrophila, enterobacteriaceae, pseudomonas species, and components of the flora of the human skin. irrigation, exploration, and culture of these wounds as well as immunization of the patient against tetanus are recommended. patients with hepatic disease or chronic illness and immunocom ...19921562661
behaviour of aeromonas species in cary-blair transport medium at various temperatures. 19921563392
structural and pathogenic properties of aeromonas schubertii.we investigated the phenotypic, structural, and pathogenic properties of 11 aeromonas schubertii strains recovered from extraintestinal sites. most a. schubertii strains were autoagglutination positive, possessed a high surface charge but low hydrophobicity, and fell into one or two biogroups on the basis of carbon substrate utilization patterns. fatty acid methyl ester analysis of a. schubertii revealed this species to contain a relatively high percentage of branched fatty acids (i-13:0, i-15:0 ...19921563798
enterotoxic effects of aeromonas sobria haemolysin in a rat jejunal perfusion system identified by specific neutralization with a monoclonal antibody.investigations into the pathogenesis of aeromonas diarrhoea have demonstrated that several different cell-free products of motile aeromonads show enterotoxic activity in suckling mouse, rat, and rabbit assay systems. the relative contributions made by separate cytotoxic and cytotonic activities in the mixture produced by in vitro culture remains unresolved. using a modified rat jejunal perfusion assay, we have studied the effects of a. sobria culture filtrates containing defined levels of haemol ...19921564437
isolation of the leucine aminopeptidase gene from aeromonas proteolytica. evidence for an enzyme precursor.the leucine aminopeptidase of aeromonas proteolytica (ec is a monomeric metalloenzyme having the capacity to bind two zn2+ atoms in the active site. structural information of this relatively small aminopeptidase that could illuminate the catalytic mechanism of the metal ions is lacking; hence, we have obtained sequences from the purified enzyme, cloned the corresponding gene, and expressed the recombinant protein in escherichia coli. the deduced primary amino acid sequence of this sec ...19921569090
haemolytic-uraemic syndrome associated with aeromonas hydrophila enterocolitis. 19921571224
dna probe for aeromonas salmonicida.a dna fragment that is specific to aeromonas salmonicida has been isolated from a genomic dna library by differential hybridization. the specificity of this fragment as a dna probe for a. salmonicida was shown by hybridization against reference strains and clinical isolates of a. salmonicida, related aeromonads, and species from several other bacterial genera. the sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two a. salmonicida cells.19921575477
virulence traits of aeromonas strains in relation to species and source of isolation.the virulence traits of 39 well-defined clinical (29 strains) and environmental (10 strains) isolates of aeromonas (16 a. hydrophila, 12 a. sobria and 11 a. caviae) were examined by a variety of assays to delineate differences, if any, in the enteropathogenic potential in relation to species and the source of isolation. the distribution of enterotoxin (ent), cytotoxin (cyt) and haemolysin (hae) producing strains of aeromonas did not correlate to species and source of isolation. the extracellular ...19921576411
elastolytic activity among aeromonas spp. using a modified bilayer plate assay.a total of 166 isolates of aeromonas, representing diverse geographical regions and originating from various sources, were evaluated for the ability to produce elastase by using a bilayer elastin agar medium (beam) plate assay. the degree of elastase activity of individual strains was roughly assessed by measuring the clear area beneath or peripheral to the colony and recorded as 1+, 2+, or 3+. of the 166 aeromonads tested, 53 (32%) were found to produce elastase, of which 26 (49%) were 3+, 21 ( ...19921582164
identification of aeromonas strains to the genospecies level in the clinical hundred thirty-three strains of aeromonas (human, n = 102; animal, n = 16; environmental, n = 15) previously identified to the dna group level by molecular methods were biochemically analyzed for 58 properties. on the basis of the use of between 9 and 16 selected tests, 132 of the 133 strains (99%) could be assigned to their correct hybridization group using this biochemical scheme. the results suggest a feasible approach for identifying aeromonads to genospecies level under appropriate cond ...19921583128
characterization of aeromonas caviae antigens which cross-react with shigella boydii and boiled cells of 16 strains of aeromonas caviae, isolated from patients with diarrhea, agglutinated with shigella boydii 5 antiserum in a slide test. further studies with seven selected strains showed agglutination with boiled cells in a tube test. lipopolysaccharide antigen extracted from one of these strains cross-reacted with s. boydii 5 in enzyme-linked immunosorbent assay and immunoblot studies. either all or the majority of the seven strains possessed properties deemed to be diarrh ...19921583145
susceptibility to antimicrobial agents and plasmid carrying in aeromonas hydrophila isolated from two estuarine systems.susceptibility to various antimicrobial agents and the presence of plasmids was investigated in eleven strains of aeromonas hydrophila isolated from samples of sea water and these strains isolated from aulacomya ater. transference of resistance to escherichia coli was attempted by conjugation and transformation experiments. the strains showed multiple resistance toward beta-lactam antibiotics and susceptibility to other antimicrobial agents. five strains harboured plasmids with molecular weights ...19921593967
stereochemical and positional specificity of the lipase/acyltransferase produced by aeromonas hydrophila.aeromonas species secrete a glycerophospholipid-cholesterol acyltransferase (gcat) which shares many properties with mammalian plasma lecithin-cholesterol acetyltransferase (lcat). we have studied the stereochemical and positional specificity of gcat against a variety of lipid substrates using nmr spectroscopy as well as other assay methods. the results show that both the primary and secondary acyl ester bonds of l-phosphatidylcholine can be hydrolyzed but only the sn-2 fatty acid can be transfe ...19921599923
molecular studies on the aerolysin gene of aeromonas species and discovery of a species-specific probe for aeromonas trota species nova.a large group of aeromonads and other enteric microorganisms were assayed for the presence of the aerolysin gene with use of dna-dna hybridization. two dna fragments corresponding to the regulatory region (aerc) and the structural gene (aera) were used as probes for the detection of the aerolysin gene in these strains. sequences corresponding to the aerolysin structural gene were widespread among aeromonas isolates. in contrast, the aerc probe was much more selective, and sequences corresponding ...19921600007
case report with review of aeromonas infection.a 31-year-old woman with a. sobria infection and septicemia was reported. the patient was a known case of anll,m4 with incomplete remission following successive courses of chemotherapy. there were abscesses in the subcutaneous tissue of right knee and the liver at necropsy. leukemic cell infiltration in various organs as well as the aforementioned organs were observed. reported cases of aeromonas infection with or without diarrhea were also reviewed. according to our knowledge the present case i ...19921602265
a comparison of total and specific immunoglobulin levels in healthy atlantic salmon (salmo salar l.) and in salmon naturally infected with aeromonas salmonicida subsp. achromogenes.healthy atlantic salmon and salmon with a history of chronic natural aeromonas salmonicida subsp. achromogenes infection were compared with respect to total serum protein and the concentration and specificity of serum immunoglobulin. the immunoglobulin level was measured using competitive elisa and the specific antibody activity against aeromonas salmonicida subsp. achromogenes was measured using double sandwich elisa. significant elevation of serum protein and immunoglobulin concentration was o ...19921604799
[usefulness of studies of susceptibility of ampicillin, carbenicillin, cefalotin and colistin as a presumptive identification system for the aeromonas sp. group].we have studied the restricted antibiotic susceptibility tests for 180 strains belonging to mesophilic aeromonas sp. isolated from stools samples. strains has been identified as follows: a. caviae (64.6%), a. hydrophila (17.7%) and a. sobria (17.7%). antibiotics tested were ampicillin (10 micrograms), carbenicillin (100 micrograms), cephalotin (30 micrograms) and colistin (10 micrograms), by means of agar diffusion tests (disk-plate). we identified four different patterns of antibiotic susceptib ...19921606227
[the occurrence of aeromonads in a drinking water supply system].this study concerns with the occurrence of aeromonads, coliforms and colony counts in a drinking water supply. aeromonas contents were detected in the range of 15.0 to greater than 2,400/100 ml in the raw water samples of the man made lake. after the drinking water treatment process including fast sand filtration and chlorination aeromonads indicated in comparison to total coliforms and colony counts early and significant an after-growth of maximal 240 aeromonads/100 ml in the peripheric drinkin ...19921609555
summary of bacterial isolates from farm-reared channel catfish (1979-1988). 19921616987
toxicity of aeromonas salmonicida cells to atlantic salmon salmo salar peritoneal macrophages.several strains of aeromonas salmonicida were toxic to cultured peritoneal macrophages of atlantic salmon, salmo salar, in minimum doses of 5 x 10(6) cfu, whereas other strains were not. there was no correlation between cytotoxicity and in vivo virulence of the bacteria, the presence or absence of the two major surface components of a. salmonicida (namely, the a-layer and the o-polysaccharide chain of the lps) nor, finally, with the ability of the strains to produce the following enzymes in vitr ...19921618355
typing of aeromonas strains from patients with diarrhoea and from drinking water.aeromonas strains (187) from human diarrhoeal stools and from drinking water (263) in the netherlands were typed by three different methods. biotyping alone was found to be of little value for epidemiological studies because 84% of all strains belonged to only 10 biotypes. common biotypes could be further differentiated by serotyping. gas-liquid chromatography of cell wall fatty acid methyl esters (fame) was useful for species identification as well as for typing: 86% of all strains could be ide ...19921618720
haemolysin and enterotoxin production by aeromonas caviae isolated from diarrhoeal patients, fish and environment.beta-haemolytic activity was shown by 46 (63%) of the 73 aeromonas caviae strains isolated from diverse sources, such as diarrhoeal stools, fish ulcers and water in titres of 16-64 hu/ml. only 2 strains showed alpha-haemolytic activity and the remaining 27% of them were nonhaemolytic. live cells and culture filtrates of 60.3% of the a. caviae isolates caused accumulation of fluid in rabbit gut loops in the initial set of experiments. of the 46 strains showing beta-haemolytic activity only 34 gav ...19921619237
rapid detection of members of the family enterobacteriaceae by a monoclonal antibody.six monoclonal antibodies directed against enterobacteria were produced and characterized. the specificity of one of these antibodies (cx9/15; immunoglobulin g2a) was studied by indirect immunofluorescence against 259 enterobacterial strains and 125 other gram-negative bacteria. all of the enterobacteria were specifically recognized, the only exception being erwinia chrysanthemi (one strain tested). bacteria not belonging to members of the family enterobacteriaceae were not detected, except for ...19921622220
application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources.we have investigated the usefulness of ribotyping for the differentiation of aeromonads isolated from five patients with gastroenteritis and from the source water, treatment plant, and distribution system of a small public water supply. aeromonas hydrophila and aeromonas caviae were isolated from fecal specimens preserved in cary-blair transport medium by using blood ampicillin agar or alkaline peptone water (ph 8.4) subcultured to blood ampicillin agar plates. a. hydrophila, aeromonas sobria, a ...19921622269
cloacal flora isolated from wild black-bellied whistling ducks (dendrocygna autumnalis) in laguna la nacha, mexico.cloacal swabs from 110 adult black-bellied whistling ducks trapped at laguna la nacha, tamaulipas, mexico, were cultured to determine the prevalence of normal and potentially pathogenic bacteria. twenty-five gram-negative enterobacteria and four gram-positive cocci were isolated. the most common isolates included escherichia coli (54%), staphylococcus spp. (29%), streptococcus spp. (22%), aeromonas hydrophila (15%) enterobacter cloacae (14%), and micrococcus sp. (14%). the implications of whistl ...19921627117
fish cell lines as targets for bacterial cytotoxins. 19921634436
the aeromonas hydrophila exee gene, required both for protein secretion and normal outer membrane biogenesis, is a member of a general secretion pathway.the aeromonas hydrophila tn5-751 insertion mutant l1.97 is unable to secrete extracellular proteins, and is fragile because of defective assembly of its outer membrane. a kpni 4.1 kb fragment, which complements this mutant when supplied with an exogenous promoter, was isolated and sequenced. it contains two complete genes, exee and exef, plus fragments of two others and may form part of an operon. the exee and exef open reading frames encode 501-residue m(r) 55,882 and 388-residue m(r) 43,431 pr ...19921640836
aeromonas species: role as human pathogens. 19921642802
antibiotic susceptibilities of the vibrionaceae to meropenem and other antimicrobial agents.the in vitro activity of meropenem was compared with imipenem and other selected antimicrobial agents against 115 isolates from the family vibrionaceae. no resistance was observed with meropenem or imipenem against these isolates. however, meropenem was generally four- to 16-fold more active than imipenem against the aeromonads and vibrio cholerae. meropenem showed excellent in vitro activity against the vibrionaceae and may be useful for eradicating infections produced by these organisms.19921643823
aeromonas hydrophila: myofascial necrosis and alcoholic patient with ascites was admitted to the intensive care unit for gastrointestinal bleeding. he subsequently developed spontaneous myonecrosis of the extremities culminating in sepsis syndrome. this was a unique, non-traumatic presentation of aeromonas hydrophila soft tissue injury.19921644966
bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-rna analysis.a set of freshwater mesocosms (1.7 m3 each) was inoculated with large amounts of escherichia coli, pseudomonas putida, and their culture medium to substantially disturb the natural microbial community. to monitor microbial community dynamics, low-molecular-weight rna (5s rrna and trna) obtained directly from bacterioplankton was analyzed by using high-resolution electrophoresis. the introduced bacteria showed no significant effect on the community structure of the natural bacterial assemblage an ...19921280060
immunostimulants added to injected aeromonas salmonicida bacterin enhance the defense mechanisms and protection in rainbow trout (oncorhynchus mykiss).immunostimulants were given to rainbow trout for assaying effects on modulating non-specific defense mechanisms, specific immune response, and protection levels against pathogen challenge with aeromonas salmonicida. three drugs, levamisole (an approved veterinary drug in the usa), a quaternary ammonium compound (qac), and a short-chain polypeptide (isk) were found to affect the non-specific defense mechanism activities, which were measured by changes in circulatory neutrophil and phagocytic acti ...19921280879
purification of aeromonas hydrophila major outer-membrane proteins: n-terminal sequence analysis and channel-forming properties.four outer-membrane proteins of aeromonas hydrophila were purified and their n-terminal sequences and channel-forming properties were determined. three could be matched with proteins from other species. one was a maltoporin, as its level increased when cells were grown in maltose-containing media, and the channel it formed was blocked by maltose. another was like ompf and ompc of escherichia coli, except that its channel fluctuated much more rapidly. the third protein, which was produced in low- ...19921283000
[the typing of environmental strains of aeromonas spp]. 19921284582
[the aerobic bacterial flora of songbird nests].in the area around ober- and unterschleissheim, a medium term decrease in the singing bird population appears to be happening. therefore, material from nesting-boxes (used for nesting or sleeping) was examined for bacterial contamination. mainly gram positive bacteria were isolated which were considered to belong to the normal flora. the occurrence of streptomyces spp. is described in the nesting material of passeres probably for the first time. enterobacteriaceae represent only a fraction of th ...19921289040
[the danger of infection in inhalation rooms].there are alarming reports about high counts of legionella and pseudomonas in the aerosol of inhalation-rooms. we therefore investigated 14 institutions (6 kurhauses, 5 hospitals and 3 sanatoria for children) in schleswig-holstein, each of them at least five-fold. for that purpose the inhalant (seawater as a rule) was investigated for the total bacterial number and the number of legionella. cfu/m3 air were determined at the aerosol outlet and a qualitative search for legionella was done in the p ...19921290566
gastroenteritis outbreak at an industrial camp--british columbia. 19921291017
[an outbreak of food poisoning suspected due to aeromonas and characteristics of the isolated strains].on june 7, 1990, food poisoning with main symptoms of abdominal pain and diarrhea occurred in inuyama city, aichi prefecture. from results of bacteriological examination, three kinds of mesophilic aeromonas spp. were detected from patients, leftover foods, well water, and cookers, one of which was a. hydrophila which was shown to produce haemolysin thought to be the cause of the food poisoning. on the other hand, a. sobria and a. caviae isolated from various materials did not produce haemolysin. ...19921292745
[leg cellulitis caused by aeromonas hydrophila. medical treatment].a case of cellulitis of the leg caused by aeromonas hydrophila in a cirrhotic patient is reported. the starting point of the infection could not be determined with certainty, but a direct local inoculation during foot-baths was suspected. because of clinical signs suggestive of erysipelas, the disease was initially treated without success with penicillin g, which raises questions concerning the choice of the initial antibiotic therapy for cellulitis of the leg in immunocompromised patients, pend ...19921296475
novel structural patterns in divalent cation-depleted surface layers of aeromonas salmonicida.the fish pathogen aeromonas salmonicida possesses a regular surface layer (or a-layer) which is an important virulence determinant. the a-protein, a single bilobed protein organized in a p4 lattice of m4c4 arrangement with two morphological domains, comprises this layer. the role of divalent cations in the a-layer structure was studied to better understand a-protein subunit interactions affecting structural flexibility and function. divalent cation bridges were found to be involved in the integr ...19921296752
production of chitinase by enterotoxigenic aeromonas species isolated from clinical and environmental sources.thirty-six isolates of aeromonas, 13 from children and 23 from the environment were tested for production of chitinase in culture supernatants. thirty-four isolates of the three species (91% clinical and 96% environmental) produced constitutive chitinase. the environmental isolates elaborated significantly more (p < 0.005) of the enzyme than those from the children. four of the environmental isolates also produced inducible chitinase. all isolates of aeromonas were enterotoxic, however, the isol ...19921296938
biochemical characteristics and enterotoxicity of aeromonas species isolated from man and environment.a total of 147 isolates of aeromonas spp., of which 54 were isolated from children and adults with diarrhoea, 44 from variety of water sources and 49 from environmental sources, were tested for enterotoxin production and its correlation with biochemical characters. lysine was decarboxylated by 38% of a. hydrophila, 35% of a. sobria and 20% of a. caviae. majority strains were unable to utilise citrate as the sole source of carbon except one of a. hydrophila, 8 of a. sobria and 6 of a. caviae. bet ...19921296942
[a strain of aeromonas veronii isolated from a patient suspected of cholera].investigations were carried on a strain isolated from patient hospitalized in poland. the patient returned from odessa, a region with cholera epidemic. the strain was suspected to be vibrio cholerae, but it did not agglutinate in v. cholerae 01 serum. the aim of this study was establishment of taxonomical classification of this strain, in order to demonstrate whether it is a representative of the non-cholera vibrio group (not agglutinating) or another representative of the family vibrionaceae. t ...19921297030
hemolytic properties of some aeromonas strains.considering the possible correlation between hemolytic and enterotoxigenic properties of aeromonas strains mentioned in the literature, in the present work we studied the practical value of the hemolysis tests in the diagnosis of aeromonas strains by using comparatively the hemolysis tube tests (with goat and sheep erythrocytes suspensions) as well as the technique on blood agar in aerobic conditions. there were studied comparatively 230 aeromonas strains (different species: a. hydrophila, a. so ...19921297462
[purification and characterization of hec toxin produced by aeromonas hydrophila].an extracellular toxin produced by aeromonas hydrophila from cultured crucian carp with septicemia was detected. the toxin was purified by ammonium sulfate precipitation, deae-cellulose chromatography and sephadex g-100 gel filtration. the factor was a single polypeptide with a molecular weight of 52.5kd determined by sds-page. the heat-stable toxin possesses hemolytic, enterotoxic and cytolytic activities. the hemolytic activity on human erythrocytes was 3.81 x 10(3) hu/mg, cd50 for vero cell w ...19921299032
nucleotide sequences and characterization of haemolysin genes from aeromonas hydrophila and aeromonas sobria.extracellular haemolysin is thought to be one of the important virulence factors in aeromonas infection. two extracellular haemolysin genes (ahh3 and ahh4) from aeromonas hydrophila strain 28sa, one (ahh5) from a. hydrophila strain ah-1 and one (asa1) from aeromonas sobria strain 33 were cloned into cosmid and plasmid vector dna in escherichia coli. the nucleotide sequences of the open reading frames of ahh3 and ahh4 are both 1476 basepairs (bp), whereas ahh5 and asa1 are 1455 and 1467 bp in len ...19921302284
incidence of aeromonas isolated from diarrhoeal children and study of some virulence factors in the isolates.stool samples from 305 children with diarrhoea and equal number of age and sex matched non-diarrhoeal control children, less than 5 years of age, were examined during the period from sept 1988 to april 1989. aeromonas spp. were isolated from 37 (12.1%) diarrhoeal and 05 (1.6%) control cases. out of 37 diarrhoeal isolates 13 (35.1%) were a. hydrophila, 19 (51.1%) a. sobria and 05 (13.5%) a. caviae. all the isolated strains were tested for haem agglutination property and haemolysin production. sev ...19921303082
antimicrobial resistance of 100 serial gram-negative isolates in two intensive care determine antimicrobial resistance pattern among gram-negative bacteria isolated from suspected sources of infections in patients hospitalized in two intensive care units (icus) at siriraj hospital from september 1991 to december 1991, minimal inhibitory concentrations of one-hundred consecutive gram-negative isolates for various antimicrobials were performed using the microbroth dilution method. of all gram-negative bacterial isolates, 25 per cent were pseudomonas aeruginosa, 22 per cent aci ...19921308536
characterization of lactoferrin binding by aeromonas hydrophila.various lactoferrin preparations (iron-saturated and iron-depleted human milk lactoferrins and bovine milk and colostrum lactoferrins) were bound by aeromonas hydrophila. binding was (i) reversible (65% of bound lactoferrin was displaced by unlabeled lactoferrin), (ii) specific (lactoferrin but not other iron-containing glycoproteins such as ferritin, transferrin, hemoglobin, and myoglobin inhibited binding), and (iii) significantly reduced by pepsin and neuraminidase treatment of the bacteria. ...19921311545
outbreaks of human enteric adenovirus types 40 and 41 in houston day care centers.human enteric adenovirus (ead) types 40 and 41 cause diarrhea in young children, but little is known about their association with outbreaks of diarrhea in the child care setting. this study evaluated ead as a cause of outbreaks of diarrhea among infants and toddlers in day care centers.19921313095
mechanism of action of a cytotonic enterotoxin produced by aeromonas this study, we describe the mechanism of action of a cytotonic enterotoxin produced by two isolates of aeromonas hydrophila. isolates ssu and ah65 are of different origin and both are capable of producing either a cytotoxic enterotoxin or aerolysin. a cytotonic enterotoxin produced by diarrheal isolate ssu, which was purified and characterized in our laboratory, elevated intracellular camp and pge2 levels in cultured chinese hamster ovary (cho) cells. likewise, enterotoxic activity expressed ...19921315703
in vitro activity of lomefloxacin and other antimicrobials against bacterial enteritis pathogens.lomefloxacin is a new, difluoroquinolone. in this study, the in vitro activity of lomefloxacin against clinical isolates of a variety of bacterial species associated with acute diarrheal disease was determined and compared with that of ciprofloxacin, ofloxacin, amoxicillin, sulphamethoxazole, trimethoprim, tetracycline, and chloramphenicol. bacterial isolates were obtained from different geographical areas, including western europe and the united kingdom, southern europe, africa, the middle east ...19921319301
[appendicitis: microbial interactions and new pathogens].the authors present an exhaustive review on microbial agents of appendicitis by means of literature and personal research data. thus, a detailed analysis is made on common autochthonous agents and their pathogenetic interactions and on less common exogenous bacterial, viral, mycotic, protozoan and helminthic agents with emphasis to the role of yersinia enterocolitica. in fact this bacterium seems responsible for 3% to 8% of cases in accordance with literature and personal research data (more det ...19921323137
inhibition of lipopolysaccharide synthesis in agrobacterium tumefaciens and aeromonas salmonicida.lipopolysaccharide (lps) synthesis was inhibited, new lipid a metabolites accumulated, and growth ceased, when the plant pathogen agrobacterium tumefaciens and the fish pathogen aeromonas salmonicida were treated with an antibacterial agent which specifically inhibits ctp:cmp-3-deoxy-manno-octulosonate cytidylyltransferase (cmp-kdo synthase). the new lipid a metabolites were purified by chromatography on deae-cellulose and chemically analysed. metabolites isolated from both bacterial species con ...19921324975
roles of structural domains in the morphology and surface anchoring of the tetragonal paracrystalline array of aeromonas hydrophila. biochemical characterization of the major structural domain.the tetragonally arranged s-layer of aeromonas hydrophila contains two morphological domains. the mature s-layer protein of a. hydrophila has a subunit molecular weight of 52,000, and has been reported to contain two structural domains. here a mutant has been isolated which produces an s-layer of subunit molecular weight 38,650 as determined by sedimentation analysis. this truncated s-protein was exported via the periplasm to the cell surface, but could not self-assemble into a tetragonal array ...19921333533
molecular and genetic characterization of cytochrome oxidase-negative aeromonas salmonicida isolated from coho salmon (oncorhynchus kisutch).cytochrome oxidase variants of the bacterial fish pathogen, aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. pathogenicity of aberrant isolates to brook trout (salvelinus fontinalis) wa ...19921335520
glucose repression of pigment production in atypical isolates of aeromonas salmonicida responsible for goldfish ulcer disease.the effect of glucose and other carbohydrates on the pigmentation of three atypical isolates of aeromonas salmonicida indicated that the addition of d-glucose at a concentration of 0.1% (w/v) or more, inhibited pigment production and caused a reduction in the size of colonies. the addition of camp reversed the inhibitory effects of d-glucose on pigment production.19921336808
susceptibility of recent clinical isolates to temafloxacin (a-63004) and other antimicrobial vitro susceptibility of 1008 strains of recent clinical isolates was determined against the new aryl fluoroquinolone temafloxacin (t-167, a-63004) ciprofloxacin, norfloxacin, ampicillin, piperacillin, cephalothin, cefoxitin, ceftazidime, gentamicin, amikacin, oxacillin and vancomycin. the minimum inhibitory concentrations (mics) of temafloxacin in micrograms/ml required for > or = 90% isolates were 0.13-0.5 for enterobacter, 0.03-0.25 for escherichia coli, 0.12-0.5 for klebsiella, 0.5-1.0 for ...19921338041
studies on the infundibular cysts of the uterine tube in camel (camelus dromedarius).two hundred eighteen genital tracts of slaughtered female camels were collected and examined. infundibular cysts were observed in 35 tracts (16%); these were either unilateral (22 cases) or bilateral (13 cases) all containing fluids of different consistencies. the morphological and histological structures of the cysts were recorded. the bacteriological investigation and physicochemical analysis of cyst contents were carried out. aeromonas hydrophila was isolated from 68.5% of cases. rectal palpa ...19921339989
non-culturable aeromonas salmonicida in lake water.the survival of aeromonas salmonicida subsp. salmonicida was investigated in lake water. during a 21-day study a. salmonicida became non-culturable in sterile lake water held at 10 degrees c. the incubation of replicate samples between 5 degrees c and 25 degrees c produced similar results. the recovery of colony-forming units of a. salmonicida from different lake water systems indicated that they survived longer in water that was naturally enriched (eutrophic) or enriched with tryptone soya brot ...19921342635
characterization of aeromonas sobria tap13 pili: a possible new colonization factor.pili of aeromonas sobria tap13 were purified and characterized. the molecular mass of the pilin was estimated to be about 23 kda by sds-page. the tap13 pili were immunologically different from a. sobria ae1 pili and a. hydrophila ae6 w pili as previously reported, nevertheless all three had indistinguishable morphology and shared a high degree of homology in their n-terminal amino acid sequences. strain tap13 and its purified pili did not agglutinate human, rabbit or sheep erythrocytes. however, ...19921357078
cloning and characterization of fxp, the flexible pilin gene of aeromonas hydrophila.the flexible pilus of aeromonas hydrophila is a morphologically and biochemically unique organelle which binds eukaryotic cell surfaces and whose expression is induced by specific physiochemical conditions. fxp, the structural gene coding for the flexible pilus subunit, was localized on a 7.6kb plasmid of a. hydrophila strain ah26. a putative shine-dalgarno sequence and -10 and -35 regions were identified, a signal peptide sequence delineated, and the coding sequence compared with other bacteria ...19921360140
purification and characterization of aeromonas sobria ae24 pili: a possible new colonization factor.pili of aeromonas sobria ae24 were purified and characterized. the molecular mass of the pilin was estimated to be about 19 kda by sds-page. the ae24 pili were electrophoretically distinguishable from previously reported aeromonas hydrophila ae6 w pili and a. sobria ae1 pili, although all three had indistinguishable morphology and shared a high degree of homology in the n-terminal amino acid sequences. strain ae24 and its purified pili adhered to rabbit intestine and agglutinated human and rabbi ...19921363704
transcriptional analysis of the aeromonas salmonicida s-layer protein gene vapa.the vapa gene of aeromonas salmonicida encodes the subunit of the surface protein array known as a-layer. nucleotide sequence analysis of the 374 bp of dna immediately upstream of vapa revealed two potential promoter sequences and other possible regulatory sequences. sequencing and polymerase chain reaction analysis showed that the region was conserved in wild-type a. salmonicida. primer extension and northern (rna) blot analysis showed that vapa transcription in a. salmonicida was directed pred ...19937504668
[identification of gram negative, facultative anaerobic, oxidase positive bacilli].we identified 100 strains of gram-negative, oxidase-positive bacilli, facultative anaerobes, isolated from different human pathological products by conventional biochemical methods. from the total number of strains studied, 1 corresponded to genus vibrio, 66 to genus aeromonas, and 33 to genus plesiomonas. it is of the utmost importance to conduct surveillance studies of these microorganisms in order to investigate their circulation in cuba.19937800889
production of authentic human proapolipoprotein a-i in escherichia coli: strategies for the removal of the amino-terminal methionine.several methods were compared with respect to the production of authentic, n-terminal methionine-free proapolipoprotein a-i in engineered escherichia coli bacteria. a first approach consisted of treating the purified methionylated recombinant protein with an amino-peptidase, purified from aeromonas proteolytica. a second series of strategies was based on the construction of proapo a-i encoding cassettes carrying built-in recognition sites suitable for specific in vitro cleavage of the products w ...19937763464
aeromonas spp. can secrete escherichia coli alkaline phosphatase into the culture supernatant, and its release requires a functional general secretion pathway.aerolysin is a channel-forming protein secreted by aeromonas hydrophila. to determine if regions of aerolysin could direct the secretion of another protein, portions of aera were fused to phoa, the escherichia coli alkaline phosphatase gene and cloned into e. coli, aeromonas salmonicida, and a. hydrophila. we were surprised to find that secretion of the enzyme by both aeromonas spp. was independent of the aerolysin segments fused to it. the smallest fusion product contained only the signal seque ...19937523832
cloning and genetic analysis of the vibrio vulnificus fur gene and construction of a fur mutant by in vivo marker exchange.vibrio vulnificus infections have been associated with iron overload and preexisting liver disease. iron may play a major role in the pathogenesis of v. vulnificus infections. many virulence genes, as well as genes involved in the transport of iron by bacteria, are regulated by iron, with increased expression under low-iron conditions. in escherichia coli and vibrio cholerae, transcriptional regulation by iron depends on the fur gene. we utilized southern hybridization under low- and high-string ...19937678593
molecular characterization of dna encoding 23s rrna and 16s-23s rrna intergenic spacer regions of aeromonas hydrophila.amplification of the gene encoding 23s rrna of aeromonas hydrophila by polymerase chain reaction, with primers complementary to conserved regions of 16s and the 3'-end of 23s rrna genes, resulted in a dna fragment of approximately 3 kb. this fragment was cloned in escherichia coli, and its nucleotide sequence determined. the region encoding 23s rrna shows high homology with the published sequences of 23s rrna from other members of the gamma division of proteobacteria. the sequence of the interge ...19937681020
relatedness of a periplasmic, broad-specificity rnase from aeromonas hydrophila to rnase i of escherichia coli and to a family of eukaryotic rnases.the isolation, sequencing, and characterization of a periplasmic rnase gene from aeromonas hydrophila ah1133 is described. following subcloning of the gene on a 2.7-kb psti fragment, its direction of transcription and approximate location were determined. analysis of the nucleotide sequence reveals that the gene is 645 bp long, coding for 215 amino acid residues with a total molecular weight of 24,215. a typical leader sequence is present at the beginning of the corresponding protein. computer a ...19937685334
dimerization stabilizes the pore-forming toxin aerolysin in solution.aerolysin is a channel-forming protein secreted as a protoxin by aeromonas hydrophila. analytical centrifugation measurements showed that proaerolysin is a dimer in solution, and this was confirmed by chemical cross-linking with dimethyl suberimidate. dissociation of proaerolysin with low concentrations of sds resulted in the loss of tertiary structure, assessed by near ultraviolet circular dichroism. this was accompanied by an increase in the protein's ability to bind the hydrophobic dye 1-anil ...19937688743
distribution of surface-exposed and non-accessible amino acid sequences among the two major structural domains of the s-layer protein of aeromonas salmonicida.the tetragonally arranged crystalline surface protein array (a-layer) of the fish pathogenic bacterium aeromonas salmonicida is a virulence factor. circular dichroism studies in the presence or absence of 0.1% sodium dodecyl sulfate showed that the secondary structure of a-protein, and its 39,439 molecular weight amino-terminal trypsin-resistant peptide, were altered. in both cases alpha-helix was increased significantly at the expense of beta-structure when sds was added. western and dot immuno ...19937692070
[precooked vegetables as the possible vehicles of motile yersinia enterocolitica and aeromonas ]. 19937521181
bacterial proteins binding to the mammalian extracellular matrix.pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e. collagens, laminin, fibronectin or proteoglycans. this review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the biological significance of these interactions. the best-characterized bacterial proteins active in these interactions are the mycobacterial fibronectin-binding proteins, the fibronectin- and the col ...19937901732
aeromonas-associated gastroenteritis in egypt.aeromonas spp. including a. hydrophila, a. sobria, and a. caviae, were recovered from the feces of 88% of diarrheic egyptian children. in contrast, only 45% of nondiarrheic children contained aeromonas spp. a probable source of aeromonas spp. is from drinking water inasmuch as nine out of ten samples analysed from the district of cairo in which the children resided tested positive for aeromonas spp. enterotoxigenicity of the isolates from various sources was tested. 33% of the diarrheic samples ...19937901953
the growth and expression of virulence factors at refrigeration temperature by aeromonas strains isolated from foods.a potentially significant subset (10%, 6/61) of aeromonas strains isolated from food (milk, lamb, chicken, seafood), all a. veronii biotype sobria, were able to produce two or more exotoxins (haemolysin, enterotoxin, and cytotoxin) at 37 degrees c, and grow well at 43 degrees c. although mesophilic organisms, they grew at 5 degrees c. in addition, they could adhere to hep-2 cells when grown at 37 degrees c, or at 5 degrees c, and expressed flexible pili (possible colonization factors) in greater ...19937906137
aeromonas hydrophila epiglottitis: a case report. 19937822999
in vitro and in situ survivals of bacterial populations added to fresh water environments.the fate of aeromonas hydrophila, alcaligenes denitrificans, vibrio cholerae non-01, pseudomonas putida and four different isolates of escherichia coli in fresh river water were assessed by using different microcosms (i.e., membrane diffusion chamber and erlenmeyer flask). when water samples were incubated at 16 +/- 1 degrees c, the differences in extent of survival among test bacteria were in general not significant. if the incubation temperature was raised to 29 +/- 1 degrees c, in the in situ ...19937982366
el fenómeno suicida en cepas de aeromonas mesófilas aisladas de muestras clínicas [the suicide phenomenon in strains of mesophilic aeromonas isolated from clinical specimens].a study was carried out to determine the occurrence of the suicide phenomenon in aeromonas spp strains, isolated from clinical samples, and to establish its relationship with the clinical manifestations of diarrheal diseases. 23 strains were studied: 10, of aeromonas sobria; 7, of aeromonas hydrophila; and 6, of aeromonas caviae. all suicidal strains were isolated from patients with acute diarrheal disease. 3 out of 8, isolated from non-diarrheic feces, showed an intermediate phenotypic profile. ...19937984811
[the identification of aeromonas species isolated in cuba].100 aeromonas strains, isolated from children under 5 years with acute diarrheal diseases from various health centers of the country, were studied from january to july, 1990. using the janda-modified popoff's and veron's model, 63% of the strains were identified in species through primary tests, and 100% of the other 37% were identified in aeromonas sobria, aeromonas hydrophila, and aeromonas caviae, using also supplementary tests.19937984818
isolation and characterization of aeromonas from seafoods in taipei.a total of 124 fresh seafoods and 158 processed seafoods collected from the retail markets and supermarkets in taipei were tested for the contamination with motile aeromonas spp. of the fresh seafoods analyzed, 88% displayed the presence of aeromonas. the isolation rates of various samples were as follows: 100%, freshwater fish; 95%, seawater fish; 78%, fish fillets; 84%, shrimp and crab of the crustacea group; 83%, bivalve shellfish and 84%, non-bivalve shellfish of the mollusca group, and 100% ...19937995079
studies on the toxic and immunosuppressive effects of cadmium on the common the last couple of decades, research on cadmium extended from its toxicologic aspects to its role in environmental pollution. the toxicity of cadmium to different species of fish varies widely. the cadmium sensitivity of batches of common carp, the most important cultured fish species of hungary, was studied. lethality, histopathologic changes and effect of cadmium on the antibody response, lysozyme level and microbicidal capacity were monitored. wide variation was found in individual sensiti ...19938017244
[heterotrophic aerobic gram negative bacilli from the itata fluvial system, chile].total bacterial counts, counts of gram negative bacilli and faecal pollution were determined in samples of freshwater collected in different sampling sites of the fluvial system itata, in the eight region of chile. these stations cover the course of the system from the andes down to the pacific ocean. bacterial counts were rather low and varied according to the human population density in the sampling sites. most of the identified bacilli were nonfermenting bacilli, among which the highest numbe ...19938047731
[the biology of bacteria used to treat industrial sewage to remove heavy metals].the content of heavy metals in the water bodies increases as a result of anthropogenic effects. heavy metals exert toxical effect on hydrobionts. involved into the trophic chain they can be the cause of grave human diseases. microorganisms possess a capacity to accumulate heavy metals in their body, evoke the process of their enzymatic transformation into nontoxical compounds and adsorb them on the cell surface. when breeding bacterial strains for the biological treatment of sewage from the meta ...19938037799
pyrolysis mass spectrometry characterisation and numerical taxonomy of aeromonas spp.reference strains (2) and 29 isolates of aeromonas spp. from clinical material and environmental specimens were characterised in traditional biochemical tests, and in pyrolysis mass spectrometry, which gives data reflecting whole-cell composition. numerical taxonomic analyses of the data sets were compared with conventional identification at species level, and pathogenic potential, as inferred from the origin of the isolates. clustering with conventional test reaction patterns showed, for each o ...19938085793
[the comparative characteristics of the antibiotic sensitivity of psychrophilic and mesophilic aeromonads].the general regularities of the antibiotic susceptibility of psychrophilic and mesophilic aeromonads were determined. the antibioticograms were in general similar. still, there was observed a higher susceptibility of aeromonas salmonicida to tetracycline, chloramphenicol and rifampicin, as well as a larger number of strains susceptible to semisynthetic broad spectrum penicillins (ampicillin and carbenicillin) and cephazoline. the susceptibility to aminoglycosides was lower.19938085887
[comparison of 2 culture media for the isolation of aeromonas spp. from feces]. 19938305566
fate of the fish pathogen aeromonas salmonicida in the peritoneal cavity of rainbow trout.a model was developed to study the fate of the fish pathogen aeromonas salmonicida in vivo, inside a specialized intraperitoneal chamber implanted in rainbow trout, oncorhynchus mykiss. although normally recalcitrant to lytic agents in vitro, owing to the presence of its regular surface array (s layer), a. salmonicida was rapidly killed in the peritoneal cavity by a host-derived, soluble lytic activity present in peritoneal fluid. peritoneal fluid was also found to kill other bacteria and lyse v ...19938306206
[microbial colonization of the caviar of the sturgeon fishes].samples of black caviar in 47.7% of cases are contaminated by opportunistic bacteria aeromonas, proteus, vibrio, v. parahaemolyticus. standardization of these micro-organisms content in black caviar is recommended.19938307402
cloning and characterization of three hemolysin genes from aeromonas salmonicida.two hemolysin genes (ash3 and ash4) of aeromonas salmonicida strain 17-2 and one (ash1) of a. salmonicida atcc14174 were cloned into the cosmid vector charomid 9-36 in escherichia coli dh1. the overall amino acid sequence of the ash3 was similar to that of the aerolysins of aeromonas hydrophila and aeromonas sobria, and hemolysins ahh3, ahh4, and ahh5 of a. hydrophila, and hemolysin asa1 of a. sobria. the sequence of ash4 was similar to that of the ahh1 hemolysin of a. hydrophila. the ash4 hemol ...19938309354
the role of a 40-megadalton plasmid in the adherence and hemolytic properties of aeromonas hydrophila.a cured strain of aeromonas hydrophila, ms-2pc, was examined for phenotypic changes in antibiotic resistance, adherence, and hemolysis. parental strain ms-2 was resistant to ampicillin, novobiocin, and carbenicillin; ms-2pc, which lacked a 40-mda plasmid, was also resistant to ampicillin but was sensitive to novobiocin and carbenicillin. the adherence of these isolates to caco-2 and hela cells was examined. ms-2pc demonstrated greater attachment to both cell lines than did strain ms-2 (p < 0.05) ...19938309357
assessment of the antimicrobial sensitivity of aeromonas salmonicida isolates from farmed atlantic salmon in scotland.sixty-five isolates of aeromonas salmonicida were assessed for antimicrobial sensitivity by disc diffusion tests and the results compared with the minimum inhibitory concentration for each isolate. the results demonstrated that disc diffusion, using a standard technique, may be used to categorize isolates as sensitive or resistant provided that the corresponding minimum inhibitory concentrations are known.19938310605
detection of aeromonas hydrophila serogroup 0:34 in faeces using an enzyme-linked immunosorbent assay.a microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of aeromonas hydrophila serogroup 0:34. the assay uses a detector antibody which shows no cross-reactions with aeromonas strains not belonging to serogroup 0:34 or non-aeromonas competing organisms. the detector antibody is mixed with the sample and incubated for 1 h; it is then microcentrifuged and the supernatant (unabsorbed antibody) titered on a microtiter plate coated with a. hydrophila c ...19938315251
campylobacter-associated diarrhoea in egyptian infants: epidemiology and clinical manifestations of disease and high frequency of concomitant infections.a diarrhoeal disease survey in alexandria, egypt determined the prevalence, seasonality, and household risk factors for campylobacter-associated diarrhoea in young children. the study population was 880 children (mean age = 9.8 months) presenting with diarrhoea at one of two hospitals. a control group consisted of 1,079 healthy children (mean age = 8.8 months) attending two nearby vaccination clinics. the overall isolation frequencies for campylobacter spp. were 16.8% for cases and 6.4% for the ...19938315255
molecular cloning and characterization of 13 out genes from erwinia carotovora subspecies carotovora: genes encoding members of a general secretion pathway (gsp) widespread in gram-negative bacteria.the chemical mutagen ethylmethanesulphonate (ems) has been used to generate mutants of erwinia carotovora subspecies carotovora which are defective in the secretion of pectinases (pel) and cellulases (cel) but unaltered for protease (prt) secretion. such mutants, called out-, still synthesize pel and cel but these enzymes accumulate within the periplasm. cosmid clones carrying wild-type e. carotovora ssp. carotovora dna, identified by their ability to restore the out+ phenotype when transferred ...19938326859
high specificity of cpha-encoded metallo-beta-lactamase from aeromonas hydrophila ae036 for carbapenems and its contribution to beta-lactam resistance.the aeromonas hydrophila ae036 chromosome contains a cpha gene encoding a metallo-beta-lactamase highly active against carbapenem antibiotics. this enzyme was induced in strain ae036 to the same extent by both benzylpenicillin and imipenem. when the cpha gene was inserted into plasmid pacyc184, used to transform escherichia coli dh5 alpha, the mics of imipenem, meropenem, and penem hre664 for recombinant clone dh5 alpha(paa20r), expressing the aeromonas metallo-beta-lactamase, were significantly ...19938328781
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