Publications

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membrane filter procedure for enumeration of aeromonas hydrophila in fresh waters.a membrane filter method (ma) for the enumeration of aeromonas hydrophila in natural water samples was developed. the complex, primary medium employs trehalose as a fermentable carbohydrate and ampicillin and ethanol as selective inhibitors. after 20 h of incubation at 37 degrees c, an in situ mannitol fermentation test followed by an in situ oxidase test is used to further differentiate a. hydrophila from other aquatic and terrestrial microorganisms present in freshwaters. the primary medium de ...1979485147
carbenicillin plus cefamandole in the treatment of infections in patients with cancer.the combination of carbenicillin plus cefamandole was administered to 88 patients with cancer during 116 evaluable episodes of fever. the overall response rate to carbenicillin plus cefamandole for the 116 episodes was 57%. there were 60 documented infections, of which 60% responded to this combination of antibiotics. the response rate was only 43% in patients with pneumonia. the etiologic agent was identified during 38 infections, of which 74% responded to carbenicillin plus cefamandole. respon ...1978649998
distribution of aeromonas hydrophila in natural and man-made thermal effluents.densities of aeromonas hydrophila showed distinct thermal optima (25 to 35 degrees c) and thermal maxima (45 degrees c) when measured along thermal gradients created by geothermal and nuclear reactor effluents. survival of a. hydrophila never exceeded 48 h at temperatures of greater than 45 degrees c. thermophilic strains could not be isolated at any site.1979485151
[characterization of aeromonas strains isolated from fresh water fishes and various reptiles]. 1979532476
[activity of antibiotics and heavy metals against "aeromonas" isolated from aquatic environments: phenotypic and genetic transfers (author's transl)].in this study, 64% of the 197 strains of aeromonas isolated from different stages of aquatic environment (surface water, sewage...) were resistant to one or two antibiotics, and 20% to four or more antibiotics. these multiresistant strains were only isolated from sewage and their resistances were transferable by conjugation to different species of gram-negative bacilli. the incidence of these results is discussed.1979533074
furunculosis of salmonids: vaccination attempts in rainbow trout (salmo gairdneri) by formalin-killed germs.two immunization trials were performed in rainbow trout, using a formalin killed aeromonas salmonicida suspension. in the first experiment, 200 g fish were administered orally or intraperitoneally. circulating antibodies were elicited in injected animals only, but no protection was found when they were challenged with a highly virulent strain. rough and smooth bacteria have provided similar agglutinins titers. in the second study, fingerlings were vaccinated by injection, but experimental diseas ...1979539775
bacterial flora of the schistosome vector snail biomphalaria glabrata.the aerobic heterotrophic bacterial flora in over 200 individuals from 10 wild populations and 3 laboratory colonies of the schistosome vector snail biomphalaria glabrata was examined. internal bacterial densities were inversely proportional to snail size and were higher in stressed and laboratory-reared snails. the numerically predominant bacterial genera in individual snails included pseudomonas, acinetobacter, aeromonas, vibrio, and several members of the enterobacteriaceae. enterobacteriacea ...1979539821
rainbow trout complement fixation used for titration of antibodies against several pathogens.since it is impossible to fix guinea pig complement (c) with rainbow trout (salmo gairdneri) antibodies, whereas it is possible with brown trout (salmo trutta) antibodies, a c fixation test has been designed which uses haemolysin and c from rainbow trout. immunization of trout against lysed sheep red blood cells (srbc) elicited production of haemolytic igm. normal trout serum (nts) can be used as a source of c at a dilution at which its "natural" haemolytic activity (against various homeotherms ...1979547824
[effect of water temperature on the development of aeromoniasis in fish]. 1978695304
[isolation of aeromonas hydrophila in human infections (author's transl)]. 1979551504
[effect of gentamycin in combination with prodigiozan on the immunological reactivity of the body].the effect of gentamicin sulphate and its combination will prodigiozan on antibody formation in experiments and the levels of the immunobiologic reactivity of patients with purulent inflammatory processes was studied with a purpose of developing rational schemes of antibiotic therapy of infectious diseases. a decrease in the titers of the antibodies to aeromonas and the number of antibody-forming cells in the spleen was noted on repeated administration of gentamicin to albino mice in a dose of 2 ...1979573585
[taxonomic studies on aeromonads of milk, water and minced meat (author's transl)].365 aeromonads isolated from milk, water and minced meat were tested in 24 biochemical characters. by application of a numerical method the material was devided into two subgroups. the properties of these subgroups are not in accordance with the definitions of species and subspecies of the taxonomical system of aeromonas as it is accepted at present. with regard to the priority of the name a. punctata it is suggested to make use of only this name till the real combinations of characters and the ...1977576341
freshwater wound infection due to aeromonas hydrophila. 1977577949
enterotoxigenic aeromonas hydrophila from a case of piglet diarrhoea. 1978742264
aeromonas hydrophila sepsis in a patient undergoing hemodialysis therapy.aeromonas hydrophila septicemia complicated by a generalized cutaneous vasculitis developed in a patient receiving home hemodialysis therapy. because the aeromonas organism is found in many natural water sources, the possibility that this patient's hemodialysis system became contaminated was explored. although cultures from the patient's home environment showed no aeromonas sp, the possibility still exists that the site of contamination was in the dialysis system.1978579373
[drinking waters and drug resistant bacteria. implications for water quality standards (author's transl)].a systematic investigation of antibiotic sensibility has been carried out on 1,000 strains of enterobacteria and aeromonas isolated from drinking water. 81,6% are resistant to one or more antibiotics. the incidence of this resistance and its epidemiological consequences are discussed.1978749057
diseases of chelonians: (2) necropsy survey of terrapins and turtles.a total of 122 terrapins (freshwater chelonians) of 36 species and seven turtles (marine chelonians) represented by three species, all of which had died in captivity, were necropsied. in terrapins, bacterial infections were a common cause of death (15.5 per cent). although salmonella infections appear to be less common in great britain than in the usa, terrapins are a potentially important source of infection to humans. fungal infections amounted to only 3.3 per cent. nutritional disorders, espe ...1978749320
effect of arginine analogues on a fish pathogen, aeromonas salmonicida.the effects of arginine analogues on a fish pathogen, aeromonas salmonicida, were investigated. this organism proved to be resistant in various degrees to most arginine analogues tested but was found to be sensitive to arginine hydroxamate. inhibition of growth by arginine hydroxamate was reversed by the addition of arginine to the bacterial culture. incorporation of 14c from 14c-amino acids, namely, guanidoarginine, glutamate, asparate, alanine, isoleucine, serine, methyl-methionine, and cystin ...1978756951
less frequently reported gram-negative fermentative rods.the clinical significance and distinguishing characteristics of less frequently reported gram-negative fermentative rods (pasteurella, yersinia, vibrio, aeromonas and plesiomonas, enterobacter agglomerans, and chromobacterium) are described. increased awareness of these organisms, a knowledge of possible sites in which they cause infection, and identification criteria which will separate them from similar bacteria will prepare the clinical microbiologist for the isolation and identification of t ...1976769548
aeromonas hydrophila infection. 1978579385
antibiotic resistance of vibrio cholerae: special considerations of r-plasmids.studies on the transmission of r plasmid by conjugation between enterobacteria and vibrio or related bacteria were reviewed. the majority of the reports confirmed successful transmission from enterobacteria to vibrio cholerae and related species, although the transmission frequencies were extremely low and the transmitted r plasmid was very unstable except for thermosensitive kanamycin plasmid and usual r plasmid coexisting with p plasmid. strains of v. cholerae and aeromonas liquefaciens as wel ...1978581573
the bactericidal action of benzoic acid and sodium acetate on the gram-negative flora of dialysis fluid.benzoic acid in concentrations from 0.18 per cent and upwards exerted a bactericidal action on the gram-negative flora of dialysis fluid at ph 4.75 in vitro. at a concentration of 0.32 per cent, a reduction of 10(5) was obtained with 24 hours. sulphonamide resistance did not affect the bactericidal action. the killing effect was hardly more than directly proportional to the concentration of benzoic acid. increasing inoculum decreased the bactericidal effect. therefore, benbzoic acid should not b ...1976788461
starvation effects on escherichia coli and aquatic bacterial responses to nutrient addition and secondary warming stresses.a gram-negative polar flagellated rod, isolated from a colorado mountain stream and considered to be an aeromonas sp., a mixed aquatic microbial population, and a culture of escherichia coli were starved to determine their responses to the short-term presence of nutrients and mild warming stress (49.5 c for 2 min) in relation to starvation time. at the beginning of the starvation period, the aeromonas isolate was extremely sensitive to the secondary warming stress. this response was markedly dim ...1976793521
[immunofluorescence technique applied at serological diagnosis of the respiratory tract infections from gram-negatives bacteria (author's transl)].the immunofluorescence indirect technique was evaluated for detecting specificities, ig classes, and levels of serum and sputum antibodies against bacteria isolated from sputa in patients with broncho-pulmonary infections. results obtained with the fa method were compared with those obtained with a passive haemagglutination test. the former method gave much more reliable results than the latter. by using fa indirect method it was possible to assess pathogenicity of baceterial strains isolated fr ...1976797326
[2 choleriform syndromes caused by aeromonas hydrophila observed in western africa]. 1977589736
chondroitinase-producing bacteria in natural habitats.a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...1975803822
the occurrence of pseudomonas spp. in surface water and in tap water as determined on citrate media.citrate-utilizing bacteria were counted in 289 samples of tap water derived from either surface water or ground water and in 32 samples of raw or partially treated surface water by using media containing ferric ammonium citrate as the carbon and energy source. the citrate-utilizing bacteria constituted only small minorities of the colony counts on lab-lemco agar at 25 c in both tap water and surface water. a total of 1071 isolates were obtained, of which 979 were able to utilize citrate. charact ...1977596862
rapid detection and identification of enteric bacteria from blood cultures.the bactec radiometric system and inolex enteric i card were used in conjunction for the rapid detection and presumptive identification of enteric gram-negative rods in blood cultures. excellent correlation was obtained between the inolex card and routine identification techniques when organisms from both artificially inoculated and clinical blood cultures were studied. in many instances, the culture report was available less than 24 hr after receipt of the specimen.1975804025
in-vitro effect of edta-tris-lysozyme solutions on selected pathogenic bacteria.the in-vitro effect of edta-tris-lysozyme solution on 16 pathogenic bacteria of medical or veterinary importance was determined. marked decreases in bacterial count occurred with pseudomonas aeruginosa, escherichia coli, moraxella osloensis and campylobacter fetus, and smaller decreses with salmonella typhimurium, shigella boydii, aeromonas hydrophila, proteus mirabilis, listeria monocytogenes and erysipelothrix insidiosa. the test solution had no effect on klebsiella ozaenae, brucella canis, co ...1975805241
brown- and red-pigmented pseudomonas aeruginosa: differentiation between melanin and pyorubrin.the pigment produced by three clinically isolated strains of pseudomonas aeruginosa has been shown to by pyomelanin. the pigment is also produced by three strains of the same species labelled as "var. erythrogenes" by the national collection of type cultures, and by aeromonas salmonicida. melanin and pyorubrin may be distinguished by the differential effects of tyrosine and glutamate on their production in minimal salts medium; furunculosis agar is a suitable medium for differentation of these p ...1975805242
[therapeutic effect of lividomycin on infections of the respiratory tract (author's transl)].therapeutic effect of lividomycin was observed in patients with infections of the respiratory tract (infections in association with bronchiectasis, mixed infections of cavities after negative conversion of tubercle bacilli, pneumonia, and so forth). significant improvement of clinical symptoms was observed in 15 cases and no improvement in 4 cases. lividomycin was administered at a dose of 1 g/day for 3 to 15 days. staphylococcus aureus (9 cases), klebsiella pneumoniae (1 case), alpha-hemolytic ...1975807755
[investigations on the standardization of polytropic substances used in microbiological test procedures. i. suitability of vitamin-free casein hydrolysates as standardizable source of n and c for bacterial growth (author's transl)].comparative testing of vitamin-free casein hydrolysates produced by merck, difco and sheffield showed that the products are evidently fairly well standardised by the manufacturing specifications and satisfy requirements as polytropic source of n and c for microbiological test procedures in which high degree of reproducibility is demanded. tests were conducted with strains of e. coli, a. punctata, p. aeruginosa and myxobacter sp. and showed that differences within the trial groups in the colony c ...1975808926
methyl violet: a selective agent for differentiation of klebsiella pneumoniae from enterobacter aerogenes and other gram-negative organisms.three selective media for differentiation of klebsiella pneumoniae from enterobacter aerogenes on the basis of colonial morphology were evaluated. using methyl violet 2b as a selective agent, strains of k. pneumoniae isolated from urine, fresh water, and fresh produce were tested against other members of enterobacteriaceae in addition to strains of aeromonas hydrophila and pseudomonas aeruginosa. comparison of colonial morphology showed k. pneumoniae produced larger, smoother colonies than other ...1975809008
bacteriophage typing of gram-negative rod-shaped bacteria. 1975811424
enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity.in or on agar media, low-density seedings of enterobacteria fail to grow in the presence of certain concentrations of ethylene diamine-di-orthohydroxyphenyl acetic acid (edda); on the other hand, high-density seedings not only grow but secrete iron chelators which release the iron bound by the edda in the medium and stimulate the growth of low-density seedings. plates of media containing edda with low-density seedings of indicator organisms were used to survey iron-chelator production in seven e ...1975812996
possible mis-identification of the fish pathogen aeromonas salmonicida. 1975813814
[study of 50 strains of aeromonas hydrophilia isolated from fishes].a bacteriological survey of the healthy goldfish (carassius auratus) demonstrated the frequent occurrence of a. hydrophila. the biochemical characters and the sensitivity to 29 chemiotherapics and 15 antiseptics of 50 fish originating strains were studied and compared with a number of strains, implicated in human pathology. the results of this study indicate a good agreement for the biochemical characters and the sensitivity to chemiotherapics between the fish and the human strains. owing to thi ...1977603101
open fracture of the tibia and fibula complicated by infection with aeromonas hydrophila. a case report. 1979457729
interaction of pseudomonas and enterobacteriaceae plasmids in aeromonas salmonicida.we observed that aeromonas salmonicida aro200 will maintain either or both the pseudomonas r-factor, pmg1, and enterobacteriaceae r-factors. this bacterial strain, therefore, provides a unique background wherein the host ranges of pseudomonas and enterobacteriaceae plasmods overlap. co-maintenance of these plasmids resulted in behavior of plasmid aggregates that allowed transfer of r-dterminants beyond the host range of the parent plasmid. we observed that the aro200 genetic background facilitat ...1976824271
an evaluation of commercial thiosulphate citrate bile salt sucrose agar (tcbs). 1976825501
the binding site of escherichia coli ribosomal protein s4 on 16-s ribosomal rna from different bacterial species.ribosomal protein s4 of escherichia coli was bound to 16-s ribosomal rnas from several bacterial species and the complexes digested with pancreatic ribonuclease in an effort to isolate heterologous rna binding sites for protein s4. 16-s rnas from aeromonas punctata, pseudomonas fluorescens and vibrio cuneatus each gave rise to protected fragments whose electrophoretic mobility was 7s, i.e. similar to that of the fragment generated from e. coli 16-s rna using the same conditions. no comparable fr ...1976827436
[microbial pollution of the environment by herds of animals. ii. some gram-negative bacterial genera from bovine herds]. 1977616588
aeromonas hydrophila corneal ulcer.two healthy men developed acute corneal ulcers caused by aeromonas hydrophila after receiving traumatic foreign body injuries to the cornea. the ulcers resolved after treatment for gram-negative bacterial corneal ulceration. a history of contamination by water, soil, or material from an aquatic source was a significant factor in each case.1978619678
neonatal septicemia and meningitis due to aeromonas shigelloides. 1978633037
aeromonas hydrophilia sepsis in a previously healthy man. 1978633579
deoxyribonucleic acid relationships among members of the genus aeromonas.polynucleotide sequences among 24 motile and 11 non-motile aeromonads were studied by analysis of deoxyribonucleic acid - deoxyribonucleic acid (dna-dna) duplexes with endonuclease s1. in addition, dna base composition (mole % guanine and cytosine (g + c)) and relative genome sizes were determined for selected strains. large variations in genome size were found and % gc ranged from 57.1 to 62.9%. on the basis of the strains examined, the genus aeromonas consists of two genotypically legitimate g ...1979476540
pathogenicity of nonpigmented cultures of chromobacterium violaceum.nonpigmented cultures of chromobacterium violaceum have been found to be similar to pigmented cultures in their virulence for mice and the pathology of their infections. clinicians and microbiologists should be prepared to consider nonpigmented c. violaceum in their differential diagnoses of infections caused by gram-negative bacteria. the laboratorian who is not aware of this possibility is likely to erroneously identify nonpigmented strains of c. violaceum as members of closely associated gene ...1977874071
cefuroxime, a new parenteral cephalosporin: collaborative in vitro susceptibility comparison with cephalothin against 5,887 clinical bacterial isolates.cefuroxime, a new parenteral cephalosporin was compared with cephalothin by broth microdilution susceptibility testing against 5,887 routine clinical bacterial isolates in four large clinical laboratories. the minimal inhibitory concentrations (mics) of cefuroxime against the enterobacteriaceae were consistently lower than those of cephalothin. this was most striking among the enterobacter species, which were generally susceptible to cefuroxime (mic </= 8 mug/ml), but resistant to cephalothin. s ...1977883818
effects of fever on host defense mechanisms after infection in the lizard dipsosaurus dorsalis.fever has never been proven beneficial in mammals, although it enhances survival in the lizard d. dorsalis infected with aeromonas hydrophila. we examined the course of the infection and the function of host defence in febrile (41 degrees) and afebrile (35 degrees or 38 degrees) animals using this model. infected, febrile lizards had sterile blood cultures, and 1-2 logs fewer bacteria in body tissues 6-12 h after infection. granulocytes appeared early and in large numbers at the site of inoculat ...1978638032
fatal aeromonas hydrophila infection of the skin.a 62-year-old woman developed a fatal aeromonas hydrophila infection of the skin after dropping a crab trap on her leg. the area surrounding the punctures was erythematous and had several flaccid bullae. a biopsy specimen of the involved skin showed necrosis and suppuration in the dermis and subcutaneous fat. the a hydrophila sepsis resolved after treatment with gentamicin, but the patient developed secondary wound infections and died three months after injury.1978663710
a bacterial disease of the darwin's frog (rhinoderma darwini).ulceration and necrosis of the rostrum in darwin's frogs was investigated microbiologically and histopathologically. the condition was attributed to infection with aeromonas liquefaciens and, possibly, an acinetobacter sp.1978672182
[role of aeromonads in human pathology]. 1977899446
[choleriform syndromes due to aeromonas hydrophila. 2 cases]. 1977917795
bacterial flora of the sea urchin echinus esculentus.a total of 85 isolates of mesophilic, aerobic, heterotrophic bacteria were isolated from the gut, peristomial membrane, and coelomic fluid from specimens of the sea urchin echinus esculentus from the clyde sea area of scotland. these isolates were compared with 26 isolates from sand and seawater in the same locality. overall, strains of pseudomonas and vibrio predominated. gut (with an average bacterial viable count of 2 x 10(7) per 3-cm section) and coelomic fluid (which was often sterile and r ...1977921260
[identification of the causing agent of erysipelas of fishes]. 1977929917
[note on intestinal bacterial microflora of a nematode: thelazia rhodesi]. 1978568815
cytotoxic activity of aeromonas hydrophila.most strains of aeromonas hydrophila tested demonstrated cytotoxic activity on several tissue-cultured cell lines. the cytotoxin is heat-labile, non-dialyzable, and immunologically distinct from that of shigella dysenteriae and clostridium perfringens. none of the aeromonas isolates was found to be enterotoxigenic by either tissue culture or rabbit ileal loop assays.1978711344
necrosis and bacterial infection in channel catfish (ictalurus punctatus) following hypoxia.apparently sterile lesions developed in the skin and musculature of channel catfish (ictalurus punctatus) six days after fish were severely stressed by an oxygen depletion associated with a phytoplankton die-off in an 8.9 ha pond. lesions were characterized by hemorrhage and necrosis. aeromonas hydrophila was isolated from either the lesions ot internal organs of all moribund fish necropsied the day after lesions were first found. as the water quality improved the health of the fish improved. wa ...1976933317
phloroglucinol derivatives from aeromonas hydrophila. 1978721714
a survey of aquarium fishes, their foods, and environmental water for salmonellae.the presence of salmonellae in the pet shop environment, fishes, aquarium water, and in a variety of commercially prepared and natural fish foods was determined. salmonellosis attributed to turtles purchased from pet shops has been responsible for an estimated 280,000 human cases annually in the united states. the possibility that aquarium fishes, or their food and aquatic habitat might also serve as sources of human salmonellosis was deemed worthy of clarification. results of the present survey ...1976940312
[sensitivity of aeromonas punctata to antibiotics]. 1976948845
[disc-electrophoresis of the protein spectra of representatives of the genera aeromonas, comamonas and pseudomonas]. 1976961260
neuraminidase activity of nag vibrios and of some representatives of the intestinal group of bacteria.it was studied 203 strains of nag vibrios and 71 strains of different enterobacteria for the ability to produce neuraminidase. the most frequent neuraminidase producers were found among the strains isolated from humans (99 strain of 131). there was no correlation between neuraminidase production and other properties of the vibrios. the examined strains of the family enterobacteriaceae did not produce the enzyme.1978744553
reductive microbial conversion of anthracycline antibiotics.reductive conversion of several anthracycline glycosides to their 7-deoxyaglycones occurs during their microaerophilic incubation with strains of aeromonas hydrophila, citrobacter freundii, and escherichia coli. further, extracts of microaerophilically grown a. hydrophilia catalyze dpnh-dependent reductive conversion of the same compounds. anthracycline substrates cleaved by both whole cells and by the cell-free system include steffimycin, steffimycin b, nogalamycin, cinerubin a, and daunomycin. ...1976786368
effect of aeromonas salmonicida (furunculosis) infection on the incorporation of [1-14c]acetate into lipids of trout liver.[1-(14)c]acetate is incorporated into lipids more rapidly by liver from aeromonas salmonicida-infected trout than by normal trout liver, both in vitro and in vivo. the increase was, in general, about twofold to less than threefold.1976965100
ultrastructural examination of aeromonas cultured in the presence of organic lead.aeromonas sp. will methylate trimethyl lead acetate (me3pboac) to volatile tetramethyl lead (me4pb). examination of cultures grown in the presence of me3pboac revealed no major irregularities between cells of the treated and untreated cultures. some cells, however, showed evidence that intracytoplasmic materials had been leached from the cells. the lead-treated cells were interpreted to contain lead ions on the basis of energy-dispersive x-ray analysis.1976984840
[the detection of aeromonads of the "hydrophila-punctata-group" within the hygienic control of drinking water (author's transl)].the first part brings a retrospective analysis of bacteriological water examinations at single wells at which within a new one year examination period e. coli, coliforms and colony count determinations were performed and in addition the water samples were examined regarding aeromonas of the "hydrophila-punctata-group". the single wells have been divided in five groups because of the pre-testing and reexamination results and because of the local circumstances. they are summarized in the tables 1- ...1976788410
enterotoxin-producing bacteria and parasites in stools of ethiopian children with diarrhoeal disease.enterotoxinogenic bacteria were isolated from 131 (37%) of 354 ethiopian infants and children with acute gastrointestinal symptoms. only one of these isolates belonged to the classical enteropathogenic serotypes of esch. coli. two colonies from each patient were isolated and tested for production of enterotoxin by the rabbit ileal loop test, the rabbit skin test, and an adrenal cell assay. however, only 38% of the isolated enterotoxinogenic strains were esch. coli; the others belonged to klebsie ...19761008593
[aeromonads--taxonomy and nomenclature]. 1976799450
an improvement in the technique for the presumptive identification of aeromonas salmonicida. 1975803942
sources of the slow-growing pigmented water bacteria.the group of organisms often labelled as "slow-growing pigmented water bacteria" may include various species of the xanthomonas, cytophoga, pseudomonas, aeromonas and flavobacterium genera. due to their slow growth, this group is usually overlooked in the average clinical laboratory since the pigmented colonies are most easily noted on membrane filter pads or on surface streaked agar plates, but only after 5-15 days of incubation at room temperature. although present at low levels in free-flowin ...1976816754
superactivation of neutral proteases: acylation with n-hydroxysuccinimide esters.a series n-hydroxysuccinimide esters of acylamino acids previously shown to acylate and thereby increase the activity of thermolysin by several orders of magnitude (blumberg, s., and vallee, b. l. (1975), biochemistry 14, 2410) has been used to modify the related neutral proteases from bacillus subtilis, bacillus megaterium, and aeromonas proteolytica. each of these enzymes is activated to a level characteristic of the particular protein and the particular acyl group incorpporated when monitored ...1976823965
taxonomy of the family vibrionaceae considered with respect to data on the primary structure of deoxyribonucleic acid. 19761034656
media-dependent oxidase reaction in a strain of aeromonas hydrophila.a strain of aeromonas hydrophila isolated from bile was oxidase positive only when grown on nonselective media. the isolate was oxidase negative when grown on gram-negative selective and/or differential media. it is proposed that oxidase tests on gram-negative rods be performed on colonies grown on nonselective media to assure valid results.1977833265
inducible oxacillin-hydrolyzing penicillinase in aeromonas hydrophila isolated from fish.an inducible penicillinase was shown to be present in a strain of aeromonas hydrophila subsp. hydrophila isolated from freshwater fish. enzyme induction was observed with benzylpenicillin or 6-aminopenicillanic acid, and the enzyme was cell bound. the penicillinase was purified 50-fold from a crude cell extract. the molecular weight was estimated to be 23,000 by gel filtration. the ph and temperature optima for the enzyme activity were 8.0 and 35 degrees c, respectively. the penicillinase showed ...19761049517
aeromonas hydrophila in the coelomic cavity of the earthworms lumbricus terrestris and eisenia foetida. 1977858944
survival value of fever in fish. 1977859637
nalidixic acid as a selective agent for the isolation of enterobacteria from river water.enterobacteria are more resistant to nalidixic acid than the majority of other gram-negative organisms isolated from river water, so allowing their selection on macconkey agar containing nalidixic aicd. selection is further improved by anaerobic incubation which, with nalidixic acid, virtually eliminates oxidase-postivie strains such as pseudomonas or aeromonas.19761068190
bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy.bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled.19751090258
[testing of various aeromonas strains isolated from corps using the resistance test]. 19751091447
[aeromonas in surface waters]. 1977873239
[swarming phenomenon of an aeromonas spec (author's transl)].a genuine swarming phenomenon, such as has previously been known to occur in proteus, bacillus and clostridium species only, was observed in an aeromonas species. fig. 1 shows the terraced swarming zones of the aeromonas species on nutrient agar. the swarming rate, expressed as the growth of the swarming zone per time unit, was measured to be 70-120 mum/min on blood agar at 30 degrees c. the swarming could be inhibited by incubation at 37 degrees c (table 2), by low saline concentrations (table ...19751101579
[utilization of lin (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria].a multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. the medium has the following formula: bacto-peptone (difco) 5 g; yeast extract (difco) 3 g; casein tryptic digest peptone (merek) 15 g; glucose 1 g; l-lysine-monohydrochloride (merck, no. 5700) 5 g; nacl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final ph 6.6. the medium is dispensed in amounts of 5 ml into tubes of 14 x 85 mm and autoclaved at 120 degrees c for 10 ...19751101584
microbial conversion of anthracycline antibiotics. 19751102512
an immuno-india ink technique for rapid laboratory diagnosis of fish furunculosis. 1977885822
fever and survival: the role of serum iron.1. the effects of bacterial infection and temperature on serum iron levels were investigated in the lizard dipsosaurus dorsalis. 2. changes in body temperature from normal (38 degrees c) to febrile (41 degrees c) did not alter serum iron levels. injection with aeromonas hydrophila led to a significant reduction in serum iron levels, comparable to that found in mammals. this reduction in serum iron level was independent of the lizard's body temperature. 3. when grown in vitro, a. hydrophila grew ...1978678329
the rapid serological diagnosis of fish furunculosis caused by "smooth" and "rough" strains of aeromonas salmonicida. 19751113076
fever and survival.the significance of fever in response to a bacterial infection has been investigated using the lizard dipsosaurus dorsalis as an animal model. these lizards develop a fever of about 2 degrees c after injection with the bacterium aeromonas hydrophila. to determine whether this elevation in body temperature increases the resistance of the host to this infection, as measured by survival, lizards were infected with the live bacteria and placed in a neutral (38 degrees c), low (34 degrees or 36 degre ...19751114347
lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification.twelve motile aeromonas strains have been examined with respect to the hexose and heptose monosaccharide residures present in the core region of their cell wall lipopolysaccharides. these strains were divided into three distinctly separate groups on the basis of the various combinations of hexose and heptose residues. the assignment of a strain to any one of the three groups furnishes a distribution which is substantially the same as that recently reported in a computerbased numerical analysis. ...1978679071
tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes.several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ...19751116988
presence of glycerophospholipid: cholesterol acyltransferase and phospholipase in culture supernatant of aeromonas hydrophila.human erythrocyte membrane glycerophospholipids are deacylated by aeromonas hydrophila 13-h culture supernatants, resulting in the production of cholesterol ester, free fatty acid, and water-soluble phosphates. this activity appears to be due to the actions of an acyltransferase (phosphatide:cholesterol acyltransferase, ec 2.3.1 group) and a phospholipase (phosphatide acyl-hydrolase). the enzyme activities are produced simultaneously in late exponential/early stationary phase, are precipitated t ...1978681278
isolation of aeromonas sp. atcc 29063, a phenol-producing organism, from fresh haddock.attempts to isolate phenol-producing organisms from stale haddock fillets failed. several such isolates, however, were readily obtained from fresh haddock and were designated aeromonas sp. phenol was produced from l-tyrosine by these isolates.19751147610
aeromonas hydrophilia septicemia in a previously healthy man. 1978682280
comparison of selected diagnostic tests for detection of motile aeromonas septicemia in fish.cultural isolation, slide agglutination (sa), tube agglutination (ta), microagglutination (ma), and fluorescent antibody (fa) techniqes were compared as methods for detecting motile aeromonas septicemia in channel catfish (ictalurus punctatus). tests were conducted on naturally infected fish from feral populations and commercial sources, as well as on fish which were experimentally infected with a virulent culture of aeromonas hydrophila. increased levels of specific serum agglutinins to a hydro ...1978697147
the occurrence of twitching motility among gram-negative bacteria.almost 1000 strains representing well above 50 different species or groups of gram-negative bacteria were examined for twitching motility. this kind of motility was mainly found in strictly aerobic cocci and rods (viz. acinetobacter calcoaceticus, moraxella spp., neisseria gonorrhoeae, n. meningitidis, pseudomonas spp., phenon 3 of thornley that is closely related to acinetobacter, and marine, yellow-pigmented rods), but also in the facultative eikenella corrodens and in anaerobic strains presum ...19751155115
a study of bacteria contaminating refrigerated cooked chicken; their spoilage potential and possible origin.cooked chicken was allowed to spoil in a normal kitchen refrigerator (variable temperature) and at a standard 4c. after 10 days' storage, bacteria were isolated from the chicken. it was found that the numbers of organisms at variable refrigeration temperature were tenfold higher than those at a uniform 4c. in an attempt to find the sources of contamination, swabs were made of different areas of the kitchen. many of the bacteria isolated from the spoiled chicken, were also isolated from the kitch ...1978701783
characterization of three aeromonas and nine pseudomonas species by extracellular enzymes and haemolysins.strains from type culture collections and clinical isolates belonging to the aeromonas and pseudomonas genera were identified with conventional tests. production of extra-cellular enzymes and haemolysins were detected by simple plate agar methods. the following enzymes were found to be of special value for a rapid and simple classification of certain species in both genera: potease (casein and gelatin agar), lecithinase (lecithin agar), and deoxyribonuclease (dna agar). elastase, staphylolytic e ...19751170482
fever: effect of drug-induced antipyresis on survival.to determine whether the prevention of fever affects the survival of an animal infected with pathogenic bacteria, lizards (dipsosaurus dorsalis) were infected with live aeromonas hydrophila and received varying doses of sodium salicylate, an antipyretic drug. twelve lizards received identical injections of bacteria along with a nontoxic dose of sodium salicylate; five animals increased their mean body temperature at least 0.6 degrees c and survived the week, whereas seven did not develop a fever ...1976935867
letter: human infection with aeromonas species: varied clinical manifestations. 19751180435
[antigenic structure of non-agglutinating vibrios and bacteria of the genus aeromonas].the authors carried out a comparative study of the antigenic structure of nonagglutinating vibrios and the respresentatives of aeromonas genus. a number of common antigens (some of which were of protein origin) were revealed in the so-called enterotoxins of nonaggultinating vibrios and aeromonas and also in the cytoplasm and ribosomes of these strains. "enterotoxin" of aeromona s strain contained the factor of vascular permeability. the serum to "enterotoxin" of the aeromonas strain neutralized ...1976961271
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