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aeromonas flagella (polar and lateral) are enterocyte adhesins that contribute to biofilm formation on surfaces.aeromonas spp. (gram-negative, aquatic bacteria which include enteropathogenic strains) have two distinct flagellar systems, namely a polar flagellum for swimming in liquid and multiple lateral flagella for swarming over surfaces. only approximately 60% of mesophilic strains can produce lateral flagella. to evaluate flagellar contributions to aeromonas intestinal colonization, we compared polar and lateral flagellar mutant strains of a diarrheal isolate of aeromonas caviae for the ability to adh ...200415039313
differential proteomic analysis of aeromonas salmonicida outer membrane proteins in response to low iron and in vivo growth conditions.aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis, a serious infectious disease of salmonids. bacterial phenotypes are known to change in vivo compared to the in vitro state. proteomic analysis of in vivo phenotypes is usually not possible due to insufficient biomass. using an in vivo growth chamber model, the pathogenic fish bacterium a. salmonicida was cultured in pure culture in vivo in its host, the atlantic salmon, to obtain sufficient biomass to allow proteo ...200415048988
expression of chitinase-encoding genes in bacillus thuringiensis and toxicity of engineered b. thuringiensis subsp. aizawai toward lymantria dispar larvae.chitinase genes from aeromonas hydrophila and bacillus circulans no.4.1 were cloned into the plasmid phy300plk and designated as phya2 and phyb43, respectively. both plasmids were introduced into various strains of b. thuringiensis by electroporation. plasmid phyb43 was generally structurally stable, but showed lower segregrational stability than phya2 in b. thuringiensis subsp. aizawai when grown under nonselective conditions. the production of chitinase from b. thuringiensis subsp. aizawai har ...200415057461
isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in new zealand.two environmental sites in new zealand were sampled (e.g., water and sediment) for bacterial isolates that could use either arsenite as an electron donor or arsenate as an electron acceptor under aerobic and anaerobic growth conditions, respectively. these two sites were subjected to widespread arsenic contamination from mine tailings generated from historic gold mining activities or from geothermal effluent. no bacteria were isolated from these sites that could utilize arsenite or arsenate unde ...200415060729
faecal contamination indicators, salmonella, vibrio and aeromonas in water used for the irrigation of agricultural products.the faecal contamination indicators (total coliforms, faecal coliforms, escherichia coli, enterococci) and the genera salmonella, vibrio, aeromonas were investigated in water samples used for irrigation. during 4 months, 52 samples were taken. the methods used were: multiple tube fermentation method for faecal contamination indicators and membrane filtration techniques for salmonella, aeromonas and vibrio. two samples were positive for salmonella spp., fourteen for aeromonas spp. and no samples ...200415061497
diversity of kenyan soda lake alkaliphiles assessed by molecular methods.dna was extracted from water and sediment samples taken from soda lakes of the kenyan-tanzanian rift valley. dna was also extracted from microbial enrichment cultures of sediment samples. 16s rrna genes were amplified by the polymerase chain reaction and microbial diversity was studied using denaturing gradient gel electrophoresis (dgge) of 16s rdna amplicons. cloning and sequencing of single dgge bands showed that they usually contained mixed amplicons. several of the amplicon sequences had hig ...200415064991
simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray.we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ...200415070982
aeromonas hydrophila orbital cellulitis in a patient with myelodysplastic syndrome.orbital cellulitis caused by aeromonas hydrophila developed in a 73-year-old male with a history of myelodysplastic syndrome. he was admitted because of fever, general malaise, pain as well as periorbital swelling in the right eye. four days later, a yellowish pustule with purulent material was noted over right lower eyelid. aeromonas hydrophila was isolated from the discharge. after administering intravenous cefuroxime 1,500 mg every 8 hours and topical ofloxacin eye oint, his symptoms subsided ...200415077892
secretion of haemolysins and proteases by aeromonas hydrophila eo63: separation and characterization of the serine protease (caseinase) and the metalloprotease (elastase).to determine the haemolysins and proteases excreted by the virulent strain eo63 of aeromonas hydrophila grown in complex media and to then fractionate and characterize them, in particular those with elastolytic activity.200415078516
bacteriology of unshelled frozen blue swimming crab (portunus pelagicus).in this study, 45 (10 whole specimens and 35 frozen claws) frozen samples of portunus pelagicus imported into sicily (italy) from the west coast of africa were examined to assess their bacteriological characteristics and suitability for consumption. bacteriological examination was performed on two subsamples for each whole crab. the first was the body and claw muscle; the second was a pool of viscera and gills. in the case of frozen claws, each muscle claw was a sample. an aerobic plate count at ...200415083736
in vitro effect of a buffered chelating agent and neomycin or oxytetracycline on bacteria associated with diseases of fish.the antimicrobial agents used to treat bacterial fish diseases are archaic, and their uses may result in the emergence of drug-resistant bacterial strains. this study evaluated the in vitro antimicrobial activity of combinations of tricide and neomycin or oxytetracycline on common disease-causing bacteria of fish and its possible use as an alternative treatment of these diseases. tricide solutions containing of 8 mm united states pharmacopeia (usp) disodium ethylenediaminetetraacetate dehydrate ...200415264722
purification and characterization of a novel metalloprotease isolated from aeromonas caviae.a novel protease produced by aeromonas caviae was purified and characterized. the molecular weight of the protease (ap19) was estimated as 19 kda on sds-polyacrylamide gel electrophoresis. the protease activity was not inhibited completely by heating at 100 degrees c for 15 min. the proteolytic activities were inhibited by metalloprotease inhibitor. the n-terminal amino acid sequence of ap19 was vtasvsfsgrctn. ap19 did not activate aeromonas proaerolysin, and did not show fluid accumulation in t ...200415268947
aeromonas hydrophila beta-hemolysin induces active chloride secretion in colon epithelial cells (ht-29/b6).the diarrheal mechanisms in aeromonas enteritis are not completely understood. in this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier function of monolayers of human intestinal cells (ht-29/b6). ion secretion was determined as a short-circuit current (i(sc)) of ht-29/b6 monolayers mounted in ussing-type chambers. transepithelial resistance (r(t)) served as a measure of permeability. a diarrheal strain of aeromonas hydrophila (strain sb ...200415271947
spectroscopic and x-ray crystallographic characterization of bestatin bound to the aminopeptidase from aeromonas (vibrio) proteolytica.binding of the competitive, slow-binding inhibitor bestatin ([(2s,3r)-3-amino-2-hydroxy-4-phenylbutanoy]-leucine) to the aminopeptidase from aeromonas proteolytica (aap) was examined by both spectroscopic and crystallographic methods. electronic absorption spectra of the catalytically competent [co_(aap)], [coco(aap)], and [znco(aap)] enzymes recorded in the presence of bestatin revealed that both of the divalent metal ions in aap are involved in binding bestatin. the electron paramagnetic reson ...200415274616
maintenance of pathogenicity during entry into and resuscitation from viable but nonculturable state in aeromonas hydrophila exposed to natural seawater at low temperature.to investigate the fate of aeromonas hydrophila pathogenicity when cells switch, in nutrient-poor filtered sterilized seawater, between the culturable and nonculturable state.200415281936
antimicrobial activity of reuterin in combination with nisin against food-borne pathogens.antimicrobial activity of reuterin individually or in combination with nisin against different food-borne gram-positive and gram-negative pathogens in milk was investigated. reuterin (8 au/ml) exhibited bacteriostatic activity against listeria monocytogenes, whereas its activity was slightly bactericidal against staphylococcus aureus at 37 degrees c. higher bactericidal activity was detected against escherichia coli o157:h7, salmonella choleraesuis subsp. choleraesuis, yersinia enterocolitica, a ...200415282134
population patterns and antimicrobial resistance of aeromonas in urban playa lakes.bacteria belonging to the genus aeromonas are indigenous to aquatic environments. once regarded as unimportant human pathogens, reports of opportunistic infections caused by these organisms have appeared increasingly in the medical literature. to estimate the potential for human infection by aeromonas where limited water resources are being used intensively, we studied the spatial and temporal variation and incidence of antimicrobial resistance among environmental isolates of aeromonas from two ...200415284885
epidemiological and clinical characteristics of bacteraemia caused by aeromonas spp. as compared with escherichia coli and pseudomonas aeruginosa.we reviewed 75 episodes of aeromonas spp. bacteraemia observed at our institution in 1979-2002, with special reference to episodes occurring in elderly patients (> or = 65 y) and to nosocomial episodes. in addition, we compared monomicrobial bacteraemic episodes caused by aeromonas spp. (n = 54) with those caused by escherichia coli (n = 108) and pseudomonas aeruginosa (n = 108), to assess differences in clinical presentation and outcome. the 75 episodes of aeromonas spp. bacteraemia occurred ma ...200415287377
a novel strategy for the expression and purification of the dna methyltransferase, m.ahdi.biochemical and structural studies of the methylase from the type 1 1/2 r-m system ahdi require the ability to purify this multi-subunit enzyme in significant quantities in a soluble and active form. several escherichia coli expression systems were tested for their ability to produce the intact methylase but this could not be achieved in a simple co-expression system. expression experiments were optimised to produce high yields of soluble m and s subunits as individual proteins. temperature and ...200415294304
effect of different temperature downshifts on protein synthesis by aeromonas hydrophila.the psychrotrophic bacterium aeromonas hydrophila 7966 was subjected to cold shocks from 30 degrees c to 20 degrees c, 15 degrees c, 10 degrees c, or 5 degrees c, or were incubated at low temperature to determine its adaptative response. the cell protein patterns analyzed by two-dimensional electrophoresis revealed that only a few proteins were underexpressed, whereas numerous new proteins appeared with the decrease of temperature, and some others were overexpressed. among them, a few constitute ...200415297910
antimicrobial resistance in selected bacterial enteropathogens in north india.the resistance of enteropathogenic bacteria to commonly prescribed antibiotics is increasing both in developing as well as in developed countries. resistance has emerged even to newer, more potent antimicrobial agents. the present study was therefore undertaken to report the current antibiotic resistance in common bacterial enteropathogens isolated in a tertiary care hospital in north india.200415299231
structural reinvestigation of the core oligosaccharide of a mutant form of aeromonas salmonicida lipopolysaccharide containing an o-4 phosphorylated and o-5 substituted kdo reducing end group using electrospray qqtof-ms/ms.the molecular structure of the mutant form of the lipopolysaccharide of aeromonas salmonicida was determined to contain an o-4 phosphorylated and o-5 substituted kdo reducing group, and is proposed as the following: [molecular structure: see text] it was established that during the cleavage of this lps with 1% acetic acid, to release the core oligosaccharide from the lipid a portion, we obtained a degraded core oligosaccharide which eliminated its phosphate group with extreme facility. the preci ...200415302978
biological characterization of aeromonas spp. isolated from the environment.cytotoxic enterotoxin (act) is a key virulence factor in the pathogenesis of infections caused by aeromonas spp. the cytotoxic enterotoxin gene (act) was detected in 32 out of 69 environmental isolates of aeromonas spp. by hybridization with the act gene probe. to evaluate the pathogenic potential of the act gene probe-positive isolates, 32 act gene probe-positive and 31 randomly selected act gene probe-negative isolates were tested for enterotoxicity in a suckling mice assay (sma), for haemolyt ...200415310164
influence of chronic exposure to treated sewage effluent on the distribution of white blood cell populations in rainbow trout (oncorhynchus mykiss) spleen.impairment of immune function in aquatic animals has been proposed as a possible consequence of low-level contamination of surface waters with anthropogenic substances such as through the discharge of wastewater into rivers, lakes, and oceans. the study at hand investigated the effects of chronic (32 weeks) exposure to sewage treatment plant (stp) effluent on the prevalence and distribution of different leucocyte populations in spleen samples of rainbow trout (oncorhynchus mykiss). to simulate a ...200415310861
analysis of freshwater pathogens: a guide to rational empiric antibiotic coverage.fresh water injuries are often contaminated with bacteria that are not typically encountered in other wounds. their treatment should include empiric administration of appropriate antibiotics. this study identifies the most common pathogens found in the lake of the ozarks and their antibiotic sensitivity. eleven of the twelve lake water samples (92%) had a positive culture result. sixty-seven percent of lake water samples contained at least two strains of bacteria. five different bacterial specie ...200415311576
the effect of vaccination and sea water entry on immunocompetence and susceptibility to kudoa thyrsites in atlantic salmon (salmo salar l.).the effect of intraperitoneal (ip) vaccination and sea water entry (swe) on the immunocompetence of cascade atlantic salmon was investigated. smolts were ip injected with aqua health's forte trade mark vaccine (listonella (vibrio) anguillarum, listonella ordalii, vibrio salmonicida and aeromonas salmonicida) at four times (42, 238, 433 and 630 degree days, dd) prior to swe and were examined for immunocompetence. immune response measurements included mitogen-driven proliferation of peripheral blo ...200415312664
validation of a pcr for diagnosis of typhoid fever and salmonellosis by amplification of the hila gene in clinical samples from colombian patients.validation of a pcr test to detect hila gene sequences of salmonella spp. was performed in blood and faeces samples from typhoid fever and salmonellosis patients. sensitivity (s), specificity (sp), positive predictive value (ppv) and negative predictive value (npv) of the pcr in blood samples were performed by testing: 37 patients with clinical diagnosis of typhoid fever, 34 of them confirmed by isolation of s. typhi from blood cultures; 35 patients infected with other pathogens corroborated by ...200415314194
mechanisms of quinolone resistance and clonal relationship among aeromonas salmonicida strains isolated from reared fish with furunculosis.the mechanisms of resistance to quinolone and epidemiological relationships among a. salmonicida strains isolated from diseased fish in french marine farms from 1998 to 2000 were investigated. the quinolone resistance-determining regions of the gyra and parc genes of 12 clinical a. salmonicida isolates with different levels of quinolone susceptibility were sequenced. mics were determined in the presence of the efflux pump inhibitor (epi) phe-arg beta-naphthylamide and e(max) values (mic without ...200415314197
evaluation of a rapid polymerase chain reaction based identification technique for vibrio cholerae isolates.rapid and accurate identification of waterborne pathogens, such as vibrio cholerae, in drinking-water sources is important to enable effective resource management and public health protection. phenotypic systems currently being used for the identification of vibrio cholerae isolates are time-consuming and the need exists for the development of suitable molecular techniques that can offer both fast and reliable identification. during this study, isolates identified as vibrio cholerae by means of ...200415318514
microarray analysis of aeromonas hydrophila cytotoxic enterotoxin-treated murine primary macrophages.we performed microarray analyses of murine peritoneal macrophages to examine cellular transcriptional responses to a cytotoxic enterotoxin of aeromonas hydrophila. while 66% of altered genes were common to both primary macrophages and the murine macrophage cell line raw 264.7, act caused expression changes of 28 genes specifically in murine peritoneal macrophages.200415322042
external decontamination of wild leeches with hypochloric acid.medicinal leech, hirudo medicinalis, has been used in plastic and reconstructive surgery, to relieve venous congestion and to improve the microrevascularization of flaps. in many countries, wild leeches are still provided from local markets and utilised with antibiotic prophylaxies. in this research, results of identification of bacteria in the transport fluid is reported, oral and intestinal floras and the antibiograms of the identified microorganisms are investigated. also, to avoid possible i ...200415329153
role of ichthyophthirius multifiliis in the infection of aeromonas hydrophila.aeromonas hydrophila and ichthyophthirius multifiliis are important fish pathogens. to clarify their relationship, two host models, goldfish with trauma and those infected with i. multifiliis, were used to study infection kinetics of a. hydrophila 4332 tagged with plasmid pgfpuv (ah4332(gfp)). the results showed that in fish suffering from ichthyophthiriosis, the bacterial infection process was accelerated and more persistent than in the other groups. it is suggested that i. multifiliis plays an ...200415330981
production of poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) with flexible 3-hydroxyhexanoate content in aeromonas hydrophila cgmcc 0911.aeromonas hydrophila cgmcc 0911 isolated from lake water was found to be able to synthesize a polyhydroxyalkanoate (pha) copolymer (phbhhx) consisting of 3-hydroxybutyrate (hb) and 4-6 mol% 3-hydroxyhexanoate (hhx). the wild-type bacterium accumulated 49% phbhhx containing 6 mol% hhx in terms of cell dry weight (cdw) when grown on lauric acid for 48 h. when a. hydrophila cgmcc 0911 expressed the acyl-coa dehydrogenase gene ( yafh) of escherichia coli, the recombinant strain could accumulate 47% ...200412920488
development and maturation of the immune system in zebrafish, danio rerio: a gene expression profiling, in situ hybridization and immunological study.the development and maturation of the immune system in zebrafish was investigated using immune-related gene expression profiling by quantitative real-time polymerase chain reaction, in situ hybridization (ish), immunoglobulin (ig) detection by immuno-affinity purification and western blotting as well as immersion immunization experiments. ikaros expression was first detected at 1 day post-fertilization (dpf) and thereafter increased gradually to more than two-fold between 28 and 42dpf before dec ...200412962979
cloning, expression and characterization of a beta-agarase gene from a marine bacterium, pseudomonas sp. sk38.a gene (paga) encoding beta-agarase from pseudomonas sp. sk38 was cloned and expressed in escherichia coli. the structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37326 and has a signal peptide of 18 amino acids. the deduced amino acid sequence showed 57% and 58% homology to beta-agarase from pseudoalteromonas atalntica and aeromonas sp., respectively. the recombinant enzyme was purified and biochemically characterized. the enzyme had maximum activi ...200312967006
the effects of temperature, water activity and ph on the growth of aeromonas hydrophila and on its subsequent survival in microcosm water.the influence of temperature, water activity and ph on the growth of aeromonas hydrophila, and on its survival after transfer in nutrient-poor water were assessed.200312969295
misidentification of aeromonas veronii biovar sobria as vibrio alginolyticus by the vitek system.to find the cause of misidentification of aeromonads when using the vitek system.200312969502
distribution and retention of antigens of aeromonas salmonicida in atlantic salmon (salmo salar l.) vaccinated with a deltaaroa mutant or formalin-inactivated bacteria in oil-adjuvant.in this study we report the differences in distribution and retention of aeromonas salmonicida antigens after vaccination with two different vaccines. parr of atlantic salmon (salmo salar) were given intraperitoneal injections of either a commercial, monovalent furunculosis vaccine (apoject) or live, attenuated a. salmonicida (deltaaroa). fish were sampled at weeks 2, 4 and 12 post-vaccination and head kidney and spleen were collected. presence of lps and 16s rdna in isolated leukocytes were inv ...200312969656
the aeromonas salmonicida a-layer protein is an important protective antigen in oil-adjuvanted vaccines. 200312969658
[comparative in vitro activity of ertapenem against aerobic and anaerobic bacteria].the in vitro activity of ertapenem (mk-0826), a new carbapenem, was studied against 389 aerobic microorganisms (187 enterobacteriaceae, 15 aeromonas spp., 42 staphylococcus spp., 43 streptococcus spp., 15 enterococcus spp., 30 haemophilus spp., 15 moraxella catarrhalis, 12 neisseria gonorrhoeae, 15 pseudomonas aeruginosa and 15 acinetobacter spp.) and 54 anaerobic isolates (15 clostridium spp., 12 peptostreptococcus spp. and 27 fragilis group bacteroides recovered from four spanish hospitals. er ...200312973459
taxonomic study of sucrose-positive aeromonas jandaei-like isolates from faeces, water and eels: emendation of a. jandaei carnahan et al. 1992.fourteen sucrose-positive aeromonas jandaei-like isolates from fresh water and reared european eels were subjected to a polyphasic study to determine their taxonomic position. numerical taxonomy was used to analyse phenotypic data obtained for these isolates and 43 type and reference strains representative of recognized aeromonas species. the a. jandaei cluster (phenon 1) was defined at 81.6 % similarity (s(j)); this included the a. jandaei-like isolates, the sucrose-positive strain aeromonas ve ...200313130026
tolerance and starvation induced cross-protection against different stresses in aeromonas hydrophila.aeromonas hydrophila is sometimes considered as a controversial human pathogen and reported to be susceptible to food processing procedures and environmental stresses. in this study, we have shown that early stationary phase cells of a. hydrophila were readily killed during up shifts in temperature (in the range 50-70 degrees c), the course of drying (at relative humidity, temperature and brightness of the laboratory) and after 5 min exposure to 20%, 30% and 40% v/v ethanol. however, this bacter ...200312927714
three small, cryptic plasmids from aeromonas salmonicida subsp. salmonicida a449.the nucleotide sequences of three small (5.2-5.6 kb) plasmids from aeromonas salmonicida subsp. salmonicida a449 are described. two of the plasmids (pasa1 and pasa3) use a cole2-type replication mechanism while the third (pasa2) is a cole1-type replicon. insertions in the rep protein and oriv region of the cole2-type plasmids provide subtle differences that allow them to be maintained compatibly. all three plasmids carry genes for mobilization (mobabcd), but transfer genes are absent and are pre ...200312932739
genetic characterization of a novel metallo-beta-lactamase gene, blaimp-13, harboured by a novel tn5051-type transposon disseminating carbapenemase genes in europe: report from the sentry worldwide antimicrobial surveillance programme.in 2001, as part of the sentry worldwide antimicrobial surveillance programme, pseudomonas aeruginosa 86-14571a was isolated at a hospital in rome from a cancer patient with a bloodstream infection. the isolate was resistant to all antibiotics except amikacin, and displayed an imipenem mic of 64 mg/l that decreased to 8 mg/l in the presence of edta. the resistance determinant was investigated.200312951335
antimicrobial susceptibility testing of aquatic bacteria: quality control disk diffusion ranges for escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 at 22 and 28 degrees c.quality control (qc) ranges for disk diffusion susceptibility testing of aquatic bacterial isolates were proposed as a result of a multilaboratory study conducted according to procedures established by the national committee for clinical laboratory standards (nccls). ranges were proposed for escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 at 22 and 28 degrees c for nine different antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, gentam ...200312958263
analysis of exotoxins produced by atypical isolates of aeromonas salmonicida, by enzymatic and serological methods.in this study, exotoxins produced by 62 aeromonas salmonicida strains and the bacterium haemophilus piscium were analysed. enzymatic assays, zymograms and serological detection were used to monitor secretion by bacterial strains of the previously described exotoxins p1, gcat and asap1 and also the extracellular p2 metallo-gelatinase and a serine caseinase, which is different from the p1 protease and has not yet been characterized. based on the results, the strains were divided into five groups. ...200312962208
use of dead probiotic cells to control furunculosis in rainbow trout, oncorhynchus mykiss (walbaum). 200312962213
oral administration of formalin-inactivated cells of aeromonas hydrophila a3-51 controls infection by atypical a. salmonicida in goldfish, carassius auratus (l.).there has been increasing interest in the use of probiotics in aquaculture to control fish diseases (e.g. douillet langdon 1994; gildberg, mikkelsen, sandaker & ringo 1997; kennedy, tucker, neidic, vermeer, cooper, jarrell & sennett 1998; deschrijver & ollevier 2000; robertson, o'dowd, burrells, williams & austin 2000), and a diverse range of gram-positive (such as carnobacterium inhibens) (robertson et al. 2000) and gram-negative bacteria, including vibrio alginolyticus (garriques & arevalo 199 ...200312962220
antagonistic activity of aeromonas media strain a199 against saprolegnia sp., an opportunistic pathogen of the eel, anguilla australis richardson.a bacteriocin-like inhibitory substance (blis) produced by aeromonas media strain a199 inhibited the growth of saprolegnia sp. in vitro, an opportunistic pathogen isolated from affected eels, anguilla australis (richardson). the presence of blis in solid media inhibited the growth of the vegetative state of the aquatic mould as well as the germination of cysts. uninhibited growth was, however, observed in the presence of inactive blis, suggesting that the in vitro antagonism derived from the bli ...200312962224
substrate specificity, metal binding properties, and spectroscopic characterization of the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase from haemophilus influenzae.the catalytic and structural properties of divalent metal ion cofactor binding sites in the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase (dape) from haemophilus influenzae were investigated. co(ii)-substituted dape enzyme was 25% more active than the zn(ii)-loaded form of the enzyme. interestingly, mn(ii) can activate dape, but only to approximately 20% of the zn(ii)-loaded enzyme. the order of the observed k(cat) values are co(ii) > zn(ii) > cd(ii) > mn(ii) >ni(ii) approximatel ...200312962500
sequence analysis and biochemical characterisation of chromosomal cav-1 (aeromonas caviae), the parental cephalosporinase of plasmid-mediated ampc 'fox' cluster.aeromonas caviae cip 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. this strain produced a cephalosporinase (pi 7.2) and an oxacillinase (pi 8.5). the cephalosporinase gene cav-1 was cloned and sequenced. unlike a. caviae donor, escherichia coli pnce50 transformant producing cav-1 beta-lactamase was resistant to cefoxitin. the deduced protein sequence cav-1 contained 382 amino acids, and shared >96% ...200312757951
simultaneous effects of environmental factors on motile aeromonas dynamics in an urban effluent and in the natural seawater.seasonal dynamics of motile aeromonas in a treated urban effluent and in natural seawater along the sfax coast (mediterranean sea, tunisia) were measured over a year concurrently with seven environmental factors, and compared with those of faecal coliforms. counts for aeromonas from a standard plate count method, ranged from 1.48 x 10(5)cfu.100 ml(-1) to 2.2 x 10(8)cfu.100 ml(-1) in the effluent and from 7.9 x 10(3)cfu.100 ml(-1) to undetectable level in the surface marine waters. contrary to fa ...200312767289
comparison of three molecular methods for typing aeromonas popoffii isolates.three typing methods, restriction fragment length polymorphism (rflp) of the 16s-23s intergenic spacer region (isr), pcr amplification of the enterobacterial repetitive intergenic consensus (eric) and of the repetitive extragenic palindromic units (rep), were evaluated for typing 26 isolates of aeromonas popoffii from different geographical origins. when the methods were independently studied, eric showed the highest discriminatory power. when the methods were combined, the best combination of t ...200312777070
characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico.a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ...200312781953
the genus aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes.a total of 193 strains representing 14 different aeromonas genomospecies were evaluated for 63 phenotypic properties to create useful tables for the reference identification of mesophilic aeromonads. only 9 of 62 biochemical tests (14%) yielded uniform results, and the fermentation of certain carbohydrates was found to be linked to specific species. a number of unusual or aberrant properties for the genus aeromonas were also detected in the collection of 428 strains (193 in the phenotypic study, ...200312791848
[the inhibitory effect of extracts from galla chinensis on marine fouling bacteria].to observe the activity of extracts from galla chinensis with different solvents against marine fouling bacteria in vitro.200312795221
group 1 beta-lactamases of aeromonas caviae and their resistance to beta-lactam antibiotics.the contribution of beta-lactamase production to beta-lactam antibiotic resistance was examined in an aeromonas caviae mutant strain, selected in vitro by cefotaxime and derived from a wild-type strain isolated in our laboratory from crude sewage. both strains produced beta-lactamase. the mutant strain (ac7m) produced beta-lactamase constitutively, in contrast to the parental strain (ac7), which was inducible by cefoxitin. ac7m was regarded as a mutant from ac7, which over-expressed beta-lactama ...200312795407
synthesis and biological evaluation of hydroxamate-based inhibitors of glutamate carboxypeptidase ii.a series of hydroxamic acids has been prepared as potential inhibitors of glutamate carboxypeptidase ii (gcp ii). compounds based on a p1' residue (primed-side inhibitors) were more potent than those based on a p1 group (unprimed-side inhibitors). inhibitory potency of the primed-side gcp ii inhibitors was found to be dependent on the number of methylene units between the hydroxamate group and pentanedioic acid. succinyl hydroxamic acid derivative, 2-(hydroxycarbamoylmethyl)pentanedioic acid, is ...200312798312
a toll-like receptor (tlr) gene that is up-regulated in activated goldfish macrophages.an expressed sequence tag screen of a macrophage activation factor and lipopolysaccharide (lps) stimulated goldfish macrophage subtractive library generated several transcripts of a putative teleost homologue of the toll-like receptor (tlr) family. the full-length tlr cdna was sequenced and is predicted to encode a type i transmembrane protein with an extracellular domain containing leucine rich repeats and a cytoplasmic tail encoding a toll/interleukin-1 receptor domain. these findings indicate ...200312798365
a novel electrochemically active and fe(iii)-reducing bacterium phylogenetically related to aeromonas hydrophila, isolated from a microbial fuel cell.a facultative anaerobic bacterium was isolated from a mediator-less microbial fuel cell fed with artificial wastewater containing acetate and designated as pa3. the isolate was identified as a strain of aeromonas hydrophila based on its biochemical, physiological and morphological characteristics as well as 16s rdna sequence analysis and dna-dna hybridization. pa3 used glucose, glycerol, pyruvate and hydrogen to reduce fe(iii), nitrate and sulfate. cyclic voltammetry showed that pa3 was electroc ...200312799011
cellulose beads bound to cellulose binding domain-fused recombinant proteins; an adjuvant system for parenteral vaccination of fish.a recombinant form of the outer membrane protein (a-layer protein) associated with atypical aeromonas salmonicida was expressed, fused to a cellulose binding domain (cbd) isolated from clostridium cellulovorans. the resultant chimerical protein was bound to either sigmacell 20((r)) or orbicell cellulose particles. common goldfish were injected intraperitoneally with the cellulose-protein complex and blood serum antibody levels produced against a-protein were examined weekly by means of elisa. th ...200312804848
immunomodulation by dietary vitamin c in healthy and aflatoxin b1-induced immunocompromised rohu (labeo rohita).the aim of this study was to examine the immunomodulatory effect of high levels of dietary vitamin c in healthy and immunocompromised rohu (labeo rohita) treated with aflatoxin b1 (afb1). four groups of rohu were fed experimental diets containing either no vitamin c or supplemented with vitamin c at 500 ppm for 60 days. on the first day of feeding, one group fed the high vitamin c diet and one fed the vitamin c deficient diet, were injected intraperitoneally with a single doses of afb1 at 1.25 m ...200312602688
aeromonas isolates from human diarrheic stool and groundwater compared by pulsed-field gel electrophoresis.gastrointestinal infections of aeromonas species are generally considered waterborne; for this reason, aeromonas hydrophila has been placed on the united states environmental protection agency contaminant candidate list of emerging pathogens in drinking water. in this study, we compared pulsed-field gel electrophoresis patterns of aeromonas isolates from stool specimens of patients with diarrhea with aeromonas isolates from patients' drinking water. among 2,565 diarrheic stool specimens submitte ...200312603994
cloning, dna sequence, and expression of aeromonas caviae ws7b chitinase gene.a chitinase-producing bacterium, designated ws7b, was isolated from a soil sample obtained from a black-pepper plantation on bangka island, indonesia. fatty-acid methyl-ester analysis indicated that the isolate was aeromonas caviae. a chitinase gene from ws7b was cloned in a puc19-based plasmid vector, but without its natural promoter. the complete nucleotide sequence of the gene was determined, and the structural gene consisted of a 2748-bp region encoding 864 amino acids. dna sequence analysis ...200312611264
characterization of microbial communities from coastal waters using microarrays.molecular methods, including dna probes, were used to identify and enumerate pathogenic vibrio species in the chesapeake bay; our data indicated that vibrio vulnificus exhibits seasonal fluctuations in number. our work included a characterization of total microbial communities from the bay; development of microarrays that identify and quantify the diversity of those communities; and observation of temporal changes in those communities. to identify members of the microbial community, we amplified ...200312620025
detection of erythrocytes deficient in glycosylphosphatidyl-inositol anchored membrane proteins in patients with paroxysmal nocturnal hemoglobinuria by the toxin hec secreted by aeromonas hydrophila j-1.to study the feasibility of diagnosing paroxysmal nocturnal hemoglobinuria (pnh) with toxin hec, the abbreviation of hemolytic, entreotoxigenicity and cytotoxity secreted by aeromonas hydrophila j-1.200312623426
detection and characterization of the hemolysin genes in aeromonas hydrophila and aeromonas sobria by multiplex pcr.a multiplex pcr assay was designed to amplify the aeromonas hydrophila and a. veronii bv. sobria hemolysin and aerolysin genes. the assay was evaluated by using 121 clinical isolates and 7 reference strains of aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex pcr. the five genotypes were characterized as type 1 for those carrying the ahh1 gene only (36% of isolates), type 2 for those carrying the asa1 gene only (8.5% of isolates), type 3 for ...200312624028
niche-specific association of aeromonas ribotypes from human and environmental origin.a total of 88 aeromonas isolates from distinct locations and sources (39 from extraintestinal infections, 31 from diarrhoeic, ten from non-diarrhoeic faeces, all human, and eight from fresh water) were subjected to phenospecies identification, serotyping, ribotyping and detection of some virulence markers. the strains belonged to four different phenospecies marked by 19 o serogroups and 38 ribotypes. no strong correlation between these parameters was found, and no group, as defined by the typing ...200312636248
scope of potential bacterial agents of diarrhoea and microbial assessment of quality of river water sources in rural venda communities in south africa.the microbial quality of several, usually untreated, surface domestic water sources, used by rural communities in the venda region of south africa, was assessed to gauge their fitness for human consumption and to highlight the possible impact of waterborne diseases. the water sources studied were six points on the levubu river and the rivers mutale, ngwedi, tshinane, makonde, mutshindudi and mudaswali. total and faecal coliform, heterotrophic bacteria, enterococci and coliphage counts were used ...200312639006
bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates.30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ...200312644237
integron-containing incu r plasmids pras1 and par-32 from the fish pathogen aeromonas salmonicida.a 45-kb r plasmid, pras1, that confers resistance to tetracyclines, trimethoprim, and sulfonamides was isolated in 1989 from an atypical strain of the fish pathogen aeromonas salmonicida. this plasmid could be transferred by conjugation to escherichia coli with a high degree of efficiency (frequency, 0.48). the following year pras1 was isolated from a. salmonicida subsp. salmonicida in the same area. incompatibility group u plasmid pras1 contained a drug resistance-determining region of 12 kb co ...200312654659
effect of diuron on aquatic bacteria in laboratory-scale wastewater treatment ponds with special reference to aeromonas species studied by colony hybridization.six laboratory-scale wastewater treatment ponds were filled with sediment and water obtained from a reference pond (a wastewater treatment plant located in a rural environment at montel-de-gelat, puy-de-dôme, france). they were kept at 20 degrees c, with alternative light and dark periods (12 h-12 h), and fed with raw effluent supplied weekly. three of them were treated with diuron (dissolved in dmso) at a final concentration 10 mg/l, while the other three received only dmso. physico-chemical pa ...200312656266
differential expression of the enolase gene under in vivo versus in vitro growth conditions of aeromonas hydrophila.aeromonas hydrophila is an emerging human pathogen that leads to gastroenteritis and other invasive diseases. by using a murine peritoneal culture (mpc) model, we identified via restriction fragment differential display pcr (rfddpcr) five genes of a. hydrophila that were differentially expressed under in vivo versus in vitro growth conditions. the gene encoding enolase was among those five genes that were differentially up regulated. enolase is a glycolytic enzyme and its surface expression was ...200312668143
coordination geometries of metal ions in d- or l-captopril-inhibited metallo-beta-lactamases.d- and l-captopril are competitive inhibitors of metallo-beta-lactamases. for the enzymes from bacillus cereus (bcii) and aeromonas hydrophila (cpha), we found that the mononuclear enzymes are the favored targets for inhibition. by combining results from extended x-ray absorption fine structure, perturbed angular correlation of gamma-rays spectroscopy, and a study of metal ion binding, we derived that for cd(ii)1-bcii, the thiolate sulfur of d-captopril binds to the metal ion located at the site ...200312668674
influence of polarisation and differentiation on interaction of 43-kda outer-membrane protein of aeromonas caviae with human enterocyte-like caco-2 cell line.it has been recognised that adherence and invasion to host cells are important steps in the pathogenesis of entero-pathogenic bacteria, including aeromonas caviae. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interaction of a. caviae isolates to caco-2 cells in different polarisation and differentiation conditions. the adherence of a. caviae may be related to accessibility of host cell basolateral receptors. aggregative a. caviae is ...200312684708
detection and genetic analysis of group ii capsules in aeromonas hydrophila.the genetic organization and sequences of the group ii capsule gene cluster of aeromonas hydrophila ppd134/91 have been determined previously. the purified capsular polysaccharides can increase the ability of avirulent strain ppd35/85 to survive in naive tilapia serum but have no inhibitory effect on the adhesion of ppd134/91 to carp epithelial cells. in this study, the presence of group ii capsules among 33 randomly chosen a. hydrophila strains was examined by electron microscopy and genetic an ...200312686647
rotavirus type a and other enteric pathogens in stool samples from children with acute diarrhea on the colombian northern coast.the present study, conducted from march 1998 to july 2000, determined the etiology of acute diarrhea in 253 young children and infants from cartagena and sincelejo, colombia. in 253 stool samples, the following enteric pathogens were recovered: rotavirus type a (36.6%) as the major agent, salmonella spp (9.0%), shigella spp (8.0%), enteric pathogenic escherichia coli (6.0%), enteric hemorragic esc. coli (2.8%), providencia alcalifaciens (2.8%), aeromonas hydrophila (2.0%), yersinia enterocolitic ...200312687410
molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from aeromonas veronii.to clone, sequence and characterize the gene encoding the omp48, a major outer membrane protein from aeromonas veronii.200312694457
enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) via manipulating the fatty acid beta-oxidation pathway in e. coli.acyl-coa dehydrogenase gene (yafh) of escherichia coli was expressed together with polyhydroxyalkanoate synthase gene (phac(ac)) and (r)-enoyl-coa hydratase gene (phaj(ac)) from aeromonas caviae. the expression plasmids were introduced into e. coli jm109, dh5 alpha and xl1-blue, respectively. compared with the strains harboring only phac(ac) and phaj(ac), all recombinant e. coli strains harboring yafh, phac(ac) and phaj(ac) accumulated at least four times more poly(3-hydroxybutyrate-co-3-hydroxy ...200312694916
transferrin and the innate immune response of fish: identification of a novel mechanism of macrophage activation.we have previously demonstrated that a non-cytokine serum protein called transferrin was a primary activating molecule of the goldfish (carassius auratus) macrophage antimicrobial response. the ability of the enzymatically cleaved forms of this protein to modulate fish macrophage function is novel and may represent a primitive and evolutionary conserved mechanism for the induction of no response of macrophages. in the present study we confirm our earlier findings using immunoaffinity purified go ...200312697311
a 5-year study of the bacterial pathogens associated with acute diarrhoea on the island of crete, greece, and their resistance to antibiotics.during a 5-year period (1995-1999) a total of 7090 stool samples obtained from patients with acute diarrhoea, mostly community-acquired, were examined for bacterial pathogens, in the greek island of crete. one or more enteric pathogens were isolated from 987 patients (14%). salmonella enterica were the most commonly isolated bacteria (6%), followed by campylobacter spp. (4.2%), and enteropathogenic escherichia coli (epec) (1.8%). yersinia enterocolitica (0.6%), shigella spp. (0.3%), and aeromona ...200312705628
co-catalytic metallopeptidases as pharmaceutical targets.understanding the reaction mechanism of co-catalytic metallopeptidases provides a starting point for the design and synthesis of new molecules that can be screened as potential pharmaceuticals. many of the enzymes that contain co-catalytic metallo-active sites play important roles in cellular processes such as tissue repair, protein maturation, hormone level regulation, cell-cycle control and protein degradation. therefore, these enzymes play central roles in several disease states including can ...200312714052
acute gastroenteritis in children : what role for antibacterials?the aim of this article is to define the currently accepted role of antibacterials in the treatment of acute gastroenteritis in children. most cases of acute gastroenteritis in children are viral, self-limited, and need only supportive treatment. appropriate fluid and electrolyte therapy, with close attention to nutrition, remain central to therapy.antibacterial therapy serves as an adjunct, to shorten the clinical course, eradicate causative organisms, reduce transmission, and prevent invasive ...200312716215
[genetic diagnoses for bacterial pathogeneses and antimicrobiol susceptibilities]. 200312718005
incidence of haemolysin-positive and drug-resistant aeromonas hydrophila in freshly caught finfish and prawn collected from major commercial fishes of coastal south india.the incidence of aeromonas hydrophila in freshly caught finfish and prawns from four major commercial fish landing sites of coastal south india was studied for a period of one year. among 514 analysed samples of seafood (410 finfish and 104 prawn), 37% of them (37.3% of finfish and 35.6% of prawn) were contaminated with a. hydrophila. a total of 255 strains of a. hydrophila were isolated. of the total isolates, about 78.4% of them were producers of haemolysin. all strains were resistant to bacit ...200312727364
a polar flagella operon (flg) of aeromonas hydrophila contains genes required for lateral flagella expression.aeromonas spp. are pathogens of both humans and poikilothermic animals, causing a variety of diseases. certain strains are able to produce two distinct types of flagella; polar flagella for swimming in liquid and lateral flagella for swarming over surfaces. although, both types of flagella have been associated as colonisation factors, little is known about their organisation and expression. here we characterised a complete flagellar locus of aeromonas hydrophila (flg) containing 16 genes, this w ...200312732473
development of a plating medium for selection of helicobacter pylori from water samples.the goal of this study was to develop a simple plating medium to allow large-scale screening of water samples for the presence of helicobacter pylori. five conventional plating media (brain heart infusion, brucella agar, columbia blood agar base, campylobacter agar kit skirrow, and hpspa medium), each containing a commercial antibiotic supplement, were initially evaluated. eight strains selected as common waterborne organisms (acinetobacter, aeromonas, bacillus, escherichia coli, enterobacter, e ...200312732566
screening toxicity study in young carp (cyprinus carpio l.) on feed amended with fumonisin b1.fumonisin b1 (fb1) is one of several mycotoxins produced by fusarium moniliforme, a major fungal pathogen of corn and widely spread throughout the world. fb1 produces a wide range of biological effects, some of which are specific for particular organs or species and some are common to all investigated animals. in this study we have evaluated subchronic toxicosis features in young carp (cyprinus carpio l.) exposed to 0.5 and 5.0 mg fb1 kg(-1) body weight for 42 days through nutritionally balanced ...200312733635
incidence and characterisation of aeromonas spp. in environmental and human samples in southern italy.the incidence and characterisation of aeromonas species in human and environmental samples in southern italy were investigated. the results emphasize that 12.3% of the 210 examined patients carried aeromonas spp. in their faeces. these results underline the need to include aeromonas spp. in the list of routinely analysed enteropathogens in all diarrhoeal stool samples, especially in children below 10 years of age, elderly persons and immunocompromised patients.200312737206
aeromonas spp. and traveler's diarrhea: clinical features and antimicrobial resistance.traveler's diarrhea is the most common health problem of international travelers. we determined the prevalence of aeromonas spp. associated with traveler's diarrhea and analyzed the geographic distribution, clinical features, and antimicrobial susceptibility. aeromonas spp. were isolated as a cause of traveler's diarrhea in 18 (2%) of 863 patients. a. veronii biotype sobria was isolated in nine patients, a. caviae in seven patients, and a. jandaei and a. hydrophila in one patient each. aeromonas ...200312737738
[aeromonas septicemia complicated with liver cirrhosis in tokyo women's medical university]. 200312739377
the bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16s rrna genes.the composition of the initial bacterial flora of pork and the development of the flora after storage at +4 degrees c for 4 days were analysed by amplification, cloning and sequencing of 16s rdna. a total of 122 clones were obtained, with lengths of > or =400 nucleotides and > or =95% similarity to database sequences. nineteen clones were similar to sequences in database not assigned to any genera. fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones mos ...200312745230
genetic linkage of the penicillinase gene, amp, and blrab, encoding the regulator of beta-lactamase expression in aeromonas spp.aeromonas hydrophila t429125, a human clinical isolate, possesses three coordinately inducible beta-lactamases encoded by amph (class d beta-lactamase), ceph (class c beta-lactamase) and imih (class b beta-lactamase). we report that upstream of amph there are two genes, blra and blrb, encoding a putative two-component regulatory system. pcr studies revealed the same blrab-amp gene arrangement in all aeromonas spp. isolates tested; namely, aeromonas veronii bv. sobria, aeromonas jandaei, aeromona ...200312746371
development of sensitive, high-throughput one-tube rt-pcr-enzyme hybridisation assay to detect selected bacterial fish pathogens.bacterial monitoring and surveillance is critical for the early detection of pathogens to avoid the spread of disease. to facilitate this, an efficient, high-performance and high-throughput method to detect the presence of femotgram amounts of ribosomal rna from 4 bacterial fish pathogens: aeromonas salmonicida; tenacibaculum maritimum (formerly flexibacter maritimus); lactococcus garvieae; and yersinia ruckeri was developed. the system uses nucleolink strips for liquid- and solid-phase pcr in 1 ...200312747638
factors associated with the adherence and biofilm formation by aeromonas caviae on glass surfaces.knowledge on factors of aeromonas caviae promoting the formation of biofilms on surfaces.200312752817
removal of fish pathogenic bacteria in biological sand filters.documentation is required to evaluate the use of infiltration systems as an alternative method for removal of fish pathogenic bacteria in wastewater from fish-farms. this study was performed to investigate the removal of bacterial fish pathogens in biological sand filters. a second aim of the study was to evaluate the bacteria used in the study in order to find a suitable model organism for future experiments. low-strength wastewater from an inland freshwater salmonid farm was intermittently loa ...200312753839
a novel type of family 19 chitinase from aeromonas sp. no.10s-24. cloning, sequence, expression, and the enzymatic properties.a family 19 chitinase gene from aeromonas sp. no.10s-24 was cloned, sequenced, and expressed in escherichia coli. from the deduced amino acid sequence, the enzyme was found to possess two repeated n-terminal chitin-binding domains, which are separated by two proline-threonine rich linkers. the calculated molecular mass was 70 391 da. the catalytic domain is homologous to those of plant family 19 chitinases by about 47%. the enzyme produced alpha-anomer by hydrolyzing beta-1,4-glycosidic linkage ...200312755707
the efficacy of a single intraperitoneal injection of oxolinic acid in the treatment of bacterial infections in goldsinny wrasse (ctenolabrus rupestris) and corkwing wrasse (symphodus melops) studied under field and laboratory conditions.the efficacy of a single intraperitoneal injection of oxolinic acid to control an outbreak of atypical aeromonas salmonicida infection in goldsinny wrasse (ctenolabrus rupestris) and in the treatment of systemic vibriosis in corkwing wrasse (symphodus melops) was examined. in addition a field study was performed to examine the effect of medication on the survival rate of goldsinny wrasse in atlantic salmon cages. four groups of wild caught goldsinny wrasse, each of 50 fish, were treated with an ...200312755901
phylogenetic analysis of members of the genus aeromonas based on gyrb gene sequences.the phylogenetic relationships of all known species of the genus aeromonas were investigated by using the sequence of gyrb, a gene that encodes the b-subunit of dna gyrase. nucleotide sequences of gyrb were determined from 53 aeromonas strains, including some new isolates, which were also characterized by analysis of the 16s rdna variable regions. the results support the recognition of the family aeromonadaceae, as distinct from plesiomonas shigelloides and other enteric bacteria. this phylogene ...200312807216
aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in thailand.a group of seven sucrose-negative aeromonas strains (referred to as group au) isolated from the internal organs of septicaemic farmed frogs (rana rugulosa) in thailand was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (faflp) and eric-pcr fingerprinting, 16s rdna sequencing, microplate dna-dna hybridizations and extensive phenotypic characterization. comparison of faflp and eric-pcr fingerprints indicated that the group au isolates belonge ...200312807217
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