Publications

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[mesophile aeromonas gastroenteritis: value of biotyping as virulence marker]. 19902098138
isolation of motile aeromonads from foods of animal origin.the purpose of this work was to select an available medium for aeromonads isolation as well as to point out the aeromonads presence in different foodstuffs of animal origin. out of 15 tested media for aeromonas isolation, two media, were selected: dfs as control medium and agos medium, both giving superposable results. 404 samples of foodstuffs of animal origin were examined; aeromonads testing was performed qualitatively and quantitatively. 142 samples with a positivity percent of 51.40% were q ...19902101209
etiological agents of diarrhoea.two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status.19902101388
aromatic-dependent mutants of aeromonas salmonicida. 19902101485
detective work in continuous ambulatory peritoneal dialysis.we report five cases of continuous ambulatory peritoneal dialysis in which the mechanisms and sources of infection were established. we show how diligent enquiry and environmental investigation can explain the pathogenesis of infection and help in prevention by motivation of the patient.19902108222
aeromonas wound infections associated with outdoor activities--california. 19902110619
effects of incorporating ampicillin, bile salts and carbohydrates in media on the recognition and selection of aeromonas spp. from faeces.the effects of incorporating ampicillin, some bile salts and sugars into media on the primary recognition and selection of aeromonads from faeces were examined. most (88%) of the 101 aeromonas strains examined had an ampicillin mic of greater than or equal to 40 mg/l, and would be isolated on blood agar containing ampicillin 30 mg/l. the strains with an ampicillin mic of less than 40 mg/l were all of human origin and predominantly a. caviae. although ampicillin at 10, 20 or 30 mg/l in blood agar ...19902111407
antimicrobial activity of ro 24-6778, a covalent bonding of desmethylfleroxacin and desacetylcefotaxime.a new "dual action" cephalosporin (ro 24-6778), representing an ester-linked desacetylcefotaxime and desmethylfleroxacin was tested against 287 aerobic bacteria. ro 24-6778 was found to be very active (minimal inhibitory concentrations [mic90], less than or equal to 0.5 micrograms/ml) against enterobacteriaceae, streptococcus spp., and aeromonas hydrophila. moderate ro 24-6778 activity (mic90, 1-8 micrograms/ml) was demonstrated against bacillus spp., staphylococcus spp. (including oxacillin-res ...19902116952
validation of flow cytometry for rapid enumeration of bacterial concentrations in pure cultures.flow cytometry was investigated as a rapid detection and counting method for bacteria in pure cultures. a simple two-parameter detection scheme was employed: particle size was measured by forward angle light scatter and nucleic acid content by fluorescence of the dna/rna-binding dye ethidium bromide. the technique gave results that correlated exceptionally well with conventional plate counting for four species of bacteria, and concentrations in the range 10(2) to 10(7) cfu/ml. cytometric counts ...19902118900
an investigation of indirect conductimetry for detection of some food-borne bacteria.indirect conductimetry using a rapid automated bacterial impedance technique was investigated. strains of staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, aeromonas hydrophila and salmonella spp. grown in whitley impedance broth all elicited indirect conductimetric changes. these indirect conductance responses were improved by the addition of 2 g/l glucose to the medium and resulted in maximum changes of 2340-4300 ...19902126006
in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients.the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ...19902127348
acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens.the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ...19902127996
plasmid screening amongst aeromonas species and plesiomonas shigelloides isolated from subjects with diarrhoea in lagos, nigeria.fifty-three aeromonas strains and 16 plesiomonas shigelloides isolated from subjects with diarrhoea in lagos were screened for the presence of plasmids. nine (17%) of the aeromonas strains and one (6.3%) of the p. shigelloides harboured one or more plasmids, ranging in size from 2.4 to 16.8 mda. as has been documented in other enteropathogens, the possibilities are that these plasmids code for some factors to enhance the virulence of their hosts.19902128001
a new and rapid method for the assay of bacterial fatty acids using high resolution capillary gas chromatography and trimethylsulfonium hydroxide.gas-liquid chromatography of cellular fatty acids is a useful tool for the identification of bacteria. derivatization of bacterial fatty acids to methyl esters by conventional techniques is usually time-consuming and complicated. a new one-step technique using trimethyl-sulfonium hydroxide allows the direct formation of fatty acid methyl esters within 1-2 min. some random examples of profiles demonstrate that straight, branched, saturated, unsaturated, hydroxy and cyclopropyl fatty acids match c ...19902128179
[mixed culture of enterococcus faecium sf 68 with pathogenic intestinal bacterial strains].an antagonistic activity of enterococcus faecium sf 68 towards plesiomonas shigelloides, aeromonas sp., enteropathogenic escherichia coli and yersinia enterocolitica has been studied and demonstrated.19902133323
[infection of an open fracture caused by aeromonas hydrophila. a case report].with regard to a case report and the review of literature the authors point out that this uncommon but severe infection due to an aero-anaerobia bacteria, existing preferentially in stagnant or running water. this seriousness leads to the use of appropriate antibiotics instead of those usually prescribed in compound fractures, and most of the time, to the use of irrigation-stucking drainage of the wounds.19902142319
[sensitivity of enteropathogenic bacteria to furazolidone].the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ...19902152485
site-directed mutagenesis of the hole-forming toxin aerolysin: studies on the roles of histidines in receptor binding and oligomerization of the monomer.the six histidines of the channel-forming protein aerolysin have been replaced one at a time with asparagine by site-directed mutagenesis, and each of the modified proteins has been purified. three proteins had the same hemolytic activity as native toxin, but the others, those changed at his107, his132, or his332, were less able to disrupt both human and rat erythrocytes. the largest reduction in activity, more than 100-fold, was observed with the his132 mutant protein. studies with radioiodinat ...19902158347
restriction endonuclease fingerprinting analysis of canadian isolates of aeromonas salmonicida.restriction endonuclease fingerprinting (ref) analysis was used to examine total cellular dna prepared from 56 independent field isolates of the fish pathogen, aeromonas salmonicida. dna was digested singly with the restriction enzymes ecori and hindiii, and the resulting fragments separated by polyacrylamide gel electrophoresis and visualized by silver staining. the ref patterns of typical isolates of a. salmonicida subsp. salmonicida were distinct from those of a. hydrophila, a. salmonicida su ...19902159364
the in-vitro activity of piperacillin/tazobactam, ciprofloxacin, ceftazidime and imipenem against multiple resistant gram-negative bacteria.one hundred and fifty gram-negative bacterial strains including respiratory pathogens, such as haemophilus influenzae and branhamella catarrhalis, and enterobacteriaceae with known resistance to beta-lactam and other antibiotics were tested in vitro for sensitivity to piperacillin, piperacillin/tazobactam (ratio 8:1), ceftazidime, ciprofloxacin and imipenem. a 16-fold or greater reduction in the mic90 of piperacillin was achieved by the addition of tazobactam, in the respiratory pathogens, thus ...19902164513
glucose-mediated catabolite repression of the tricarboxylic acid cycle as an explanation for increased acetic acid production in suicidal aeromonas strains.growth in the presence of glucose, even under highly aerobic conditions, significantly reduced the activities of three tricarboxylic acid cycle enzymes, citrate synthetase, alpha-ketoglutarate dehydrogenase, and malate dehydrogenase, in suicidal but not nonsuicidal aeromonas strains. pyruvate dehydrogenase activity, however, was significantly increased. the activities of all of the enzymes, as well as the glucose-mediated increase in acetic acid production, were shown to be regulated by cataboli ...19902165482
particle agglutination assays to identify fibronectin and collagen cell surface receptors and lectins in aeromonas and vibrio species.a rapid particle agglutination assay (paa) utilizing latex beads coated with connective tissue and serum proteins was evaluated for its ability to identify fibronectin, collagen (types i and iv), fibrinogen, and transferrin cell surface receptors on vibrio and aeromonas strains isolated from diseased fish, human infections, and the environment. similar tests were performed to screen for cell surface lectins. vibrio as well as aeromonas strains were found to bind connective tissue proteins (colla ...19902166473
[clinical evaluation of fosfomycin].a total of 15 patients with a variety of infectious disease were treated with fosfomycin in chang gung memorial hospital, kaoshiung. the drug was administered by iv drip infusion in doses of 204g per day for 5-28 days. the clinical response was satisfactory in 6 (100%) of 6 patients with urinary tract infection, 8 (80%) of 10 with septicemia and 1 with pneumonia. overall, fosfomycin was effective in 13 (86%) of all patients treated. except for 1 isolate of the pathogen, b. fragilis, and another ...19902168262
aeromonas hydrophila water-associated traumatic wound infections: a review.the bacterium aeromonas hydrophila is commonly found in natural bodies of water, and is a potential invasive pathogen for those who suffer trauma while submerged in water. five patients treated by the authors and 18 previously reported patients were reviewed to further define the characteristics of a. hydrophila wound infections. although aeromonas has been isolated from salt and brackish water, all 23 cases occurred following contact with fresh water. signs of infection usually appeared 8-48 ho ...19902179571
aeromonas species bacteremia in nonimmunocompromised hosts. two case reports and a review of the literature.aeromonas hydrophila septicemia usually occurs in immunocompromised hosts. we present two case reports of aeromonas hydrophila septicemia in uncompromised patients from two medical centers. three additional cases (two in immunocompromised hosts and one in a normal host) were found in these centers after retrospective screening of bacteriologic records over the last 3 years. a review of the english medical literature over four decades revealed only 24 additional cases of aeromonas infection in th ...19902180851
third-generation cephalosporins in the treatment of rare infections.this article reviews the published clinical experience of the use of the third-generation cephalosporins in the treatment of rare infections. rare infections are defined as those caused by unusual pathogens or multi-resistant organisms as well as those occurring in unusual or pharmacologically protected body sites. examples of such infections are uncommon causes of meningitis and ventriculitis, brain abscess, rare causes of bacterial endocarditis, metastatic salmonella infections, spontaneous ba ...19902183606
biochemical characteristics and a simple scheme for the identification of aeromonas species and plesiomonas shigelloides.the biochemical characteristics of 55 strains of aeromonas spp. and 16 p. shigelloides of human faecal origin were examined. results of tests for the production of oxidase, acid from xylose, dulcitol, adonitol, mannitol, or inositol; greenish pigmentation on nutrient agar, and growth in 6.5% nacl broth were found to be specific and reproducible. however, other tests such as haemolysis on blood agar (ba), production of dnase, amylase, ornithine or lysine decarboxylase; citrate utilization and the ...19902190003
purification of cloned proaerolysin released by a low protease mutant of aeromonas salmonicida.the precursor to the hole-forming toxin aerolysin has been purified in high yield from culture supernatants of a mutant of aeromonas salmonicida containing the cloned structural gene. the mutant strain was generated by tn5 mutagenesis. it released little or no protease or other extracellular proteins, including phospholipase, suggesting that it is a regulatory mutant. the absence of protease allowed the isolation of protoxin free from contaminating aerolysin. typically, more than 50 mg of pure p ...19902190617
[inhibition of enteropathogens by clostridium butyricum miyairi 588].the inhibitory effect of clostridium butyricum miyairi 588 against various enteropathogens was investigated in mixed cultures. it was observed that c. butyricum m588 inhibited the growth of vibrio cholerae o1, v. cholerae non-o1, aeromonas hydrophila, and shigella flexneri. considering that the interaction between c. butyricum and shigella is especially important because of their proliferation site in the lower intestine, further examinations were carried out on shigella in particular. results w ...19902193065
molecular cloning and characterization of an extracellular protease gene from aeromonas hydrophila.a structural gene which codes for an extracellular protease in aeromonas hydrophilia so2/2 and d13 was cloned in escherichia coli c600-1 by using pbr322 as a vector. the gene codes for a temperature-stable protease with a molecular mass of approximately 38,000 daltons. the protein was secreted to the periplasm of e. coli c600-1 and purified by osmotic shock. cloned protease (p3) was identical in molecular mass and properties to the one purified from a. hydrophila so2/2 culture supernatant as an ...19902193924
acute gastroenteritis due to double infection with enteropathogenic escherichia coli or salmonella and another bacterial pathogen.two enteric bacterial pathogens were concomitantly isolated from the feces of 18 infants less than 6 months of age admitted to the assaf harofeh medical center over a 7-year period. in all but two patients stool cultures grew enteropathogenic escherichia coli, six of serogroup 0119. the other organisms cultured were shigella, salmonella, campylobacter jejuni and aeromonas hydrophila. the usual clinical presentation was diarrhea, dehydration and vomiting of acute onset, and low grade fever. patie ...19902199408
isolation and identification of autoagglutinating serogroup o:11 aeromonas strains in the clinical laboratory.we evaluated the extent to which serogroup o:11 aeromonas strains could be recovered from both clinical and environmental specimens and the cultural parameters that affected the phenotypic marker (autoagglutination) associated with this group. of over 200 aeromonas strains screened, serogroup o:11 was identified only among the phenospecies a. hydrophila and a. sobria and was associated with clinical isolates more frequently than with environmental strains. blood and wound isolates accounted for ...19902199496
production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, brazil.an elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ...19902199511
a concurrent increasing of natural antibodies and enhancement of resistance to furunculosis in rainbow trout.in order to compare the respective roles of specific response and non-specific mechanisms in the enhancement of resistance to furunculosis, 2 groups of rainbow trout (oncorhynchus mykiss) were immunized with supernatants of aeromonas salmonicida or escherichia coli cultures. the fish received 4 weekly intraperitoneal injections (70 micrograms of crude proteins). one control group was injected with phosphate-buffered saline, while another was untreated. specific agglutinins were produced only in ...19902202250
survival of aeromonas hydrophila and escherichia coli in aquatic environments.survival of aeromonas hydrophila and escherichia coli in distilled water, pond water and effluents of anaerobic digesters were examined. survival capabilities of a. hydrophila in various aquatic systems were similar to e. coli except in the raw effluents of anaerobic digesters, where a. hydrophila survived significantly better than e. coli for 6 days. pathogens such as staphylococcus aureus, salmonella enteritidis and vibrio cholerae were readily killed in the raw effluents of the anaerobic dige ...19902203637
virulence factors in motile aeromonas species. 19902204614
[the occurrence of bacteria pathogenic for humans in freshwater fish].freshwater fish are of minor importance as vectors of food-borne disease in humans. these diseases can only rarely be traced back to bacterial pathogens. however, there is a number of bacteria with facultative pathogenicity for man, which are part of the natural aquatic environment. among these bacteria, the motile aeromonads (a. sp.) have become more and more important. a review is presented on a. sp. infections which have been traced back to contact with fish or water. critical discussion of t ...19902205462
temperature as an environmental factor influencing the pathogenicity of aeromonas hydrophila.contamination of wounds by surface water and the aspiration of such water following an accident can lead to severe septic infection by a. hydrophila. as such water is often found at low temperatures, the question should be asked whether this particular water environment influences the pathogenicity of this organism. some experiments have been undertaken to assess if the pathogenicity of a. hydrophila is affected by temperature. the pathogenicity of a. hydrophila adapted to the low temperature of ...19902206202
mucosal intussusception to avoid ascending cholangitis.many methods have been devised to prevent ascending cholangitis following kasai's hepatic portoenterostomy for biliary atresia. to investigate the effectiveness of mucosal intussusception in preventing ascending cholangitis, 20 mongrel dogs were randomized to undergo roux-en-y cholecystjejunostomy and common bile duct ligation with or without mucosal intussusception. aeromonas hydrophila was instilled into the gastrointestinal tract. bacterial cultures were grown from samples taken from the gall ...19902207590
rapid presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity.a new test combination for presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity in conjunction with esculin hydrolysis and gas production from glucose was evaluated. of 40 clinical isolates of aeromonas tested statically at 30 degrees c, seven were identified as aeromonas hydrophilia (non-suicidal, aerogenic, esculin positive), eight as aeromonas sobria (suicide variable, mostly aerogenic, esculin negative), and 25 as aeromonas caviae (suicidal, anaer ...19902209629
the incidence of virulence factors in mesophilic aeromonas species isolated from farm animals and their environment.sixty-one isolates of aeromonas spp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. most isolates of aeromonas hydrophila were cytotoxic (96.4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67.9%). in contrast few of the aeromonas caviae isolates produced these three markers (13.6%, 27.3% and 36.4% respectively). in general, aeromonas sobria o ...19902209733
isolation and characterization of bacteriophage pm3 from aeromonas hydrophila the bacterial receptor for which is the monopolar flagellum.pm3 is an aeromonas-specific bacteriophage which was isolated and characterized on a. hydrophila strain tf7. spontaneous mutants resistant to pm3 were non-motile having lost their characteristic monopolar flagellum. in addition, purified flagella inactivated pm3. pm3 is the first filamentous bacteriophage isolated on aeromonas, the adsorption site for which is the monopolar flagellum.19902210340
in-vitro activity of pd 117 596, a new quinolone, against bacterial isolates from cancer patients.the in-vitro activity of pd117 596, a new 4-quinolone antimicrobial agent was compared with that of ciprofloxacin against 798 gram-positive and gram-negative distinct isolates from cancer patients. pd117 596 was found to have a broad antimicrobial spectrum with excellent activity against the enterobacteriaceae (mic90 0.03 mg/l) acinetobacter spp. (mic90 0.25 mg/l), aeromonas spp. (mic100 0.06 mg/l) and pseudomonas spp. including ps. aeruginosa (mic90 0.5 mg/l). it was also extremely active again ...19902211445
comparative study of the prevalence and clinical profiles of diarrheas due to aeromonas and other enteric pathogens.the prevalence of aeromonas spp. and other enteric pathogens in stool specimens from diarrheic and non-diarrheic patients was studied over a 12 month period (january to december, 1986). except for the absence of fever, all the clinical features in aeromonas diarrhea were comparable to those associated with other diarrheagenic agents. these features included abdominal pain (30%), vomiting (24.5%), fever (31.5%), dehydration (9.5%) and hematochezia (19.5%). aeromonas spp. were more frequently isol ...19902212640
water associated infections. 19902216140
hemolysin as a marker for serratia.all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ...19902222120
characterization of aeromonas salmonicida variants with altered cell surfaces and their use in studying surface protein assembly.aeromonas salmonicida variants were characterized for alterations in their cell surface structure and used to examine reconstitution of the surface protein layer (a-layer). variants lacking outer membrane o-polysaccharide were devoid of a-layer and excreted stainable floret-like material of the surface protein (a-protein). one variant, showing partial loss of o-polysaccharide, was associated with a disrupted a-layer and excretion of some a-protein. variants lacking a-protein but possessing o-pol ...19902222123
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...19902226498
electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative aeromonas hydrophila and aeromonas sobria.the protein and lipopolysaccharide (lps) compositions of 10 autoagglutinating aeromonas hydrophila and aeromonas sobria strains were studied; one group consisted of five serogroup o:11 strains that contained an s layer, while a second group was composed of diverse serogroups that were s layer negative by transmission electron microscopy. all serogroup o:11 strains were found to contain a predominant 52,000- to 54,000-molecular-weight protein that was present on both whole-cell and outer membrane ...19902229347
aerobic bacteria cultured from the mouth of the american opossum (didelphis virginiana) with reference to bacteria associated with bite infections.the american opossum inflicts bite injuries both when hunted for food and when accidentally provoked when handled in captivity. this study involved aerobically culturing organisms from the mouths of seven wild opossums (didelphis virginiana). isolates included streptococci, coagulase-positive and -negative staphylococci, aeromonas spp., citrobacter freundii, eikenella corrodens, and escherichia coli.19902229365
[an unusual hospital infection: aeromonas hydrophila infection due to the use of leeches].a patient is described with an infection with aeromonas hydrophila after the use of medical leeches to improve the circulation of a congested region of surgery. the use of medical leeches and the treatment of a leech-related infection are discussed.19902234189
very prolonged diarrhea associated with aeromonas hydrophila. 19902239888
production of exotoxins by aeromonas spp. at 5 degrees c.the ability of 60 strains of aeromonas to produce enterotoxin and haemolysin after cultivation at 5 degrees c for 7-10 d was investigated. the strains were isolated from lamb meat, offal, carcasses and faeces, and had previously been tested for their ability to produce these exotoxins at 37 degrees c. the results showed that some strains of aeromonas hydrophila and a. sobria were capable of producing enterotoxin and haemolysin at 5 degrees c, but none of the a. caviae strains tested produced the ...19902246140
the effect of terrestrial effluents on the incidence of aeromonas spp. in coastal waters.the numbers of aeromonas species were monitored for one year at 18 stations on the barcelona coast. their concentrations exceed those of faecal coliforms and faecal streptococci in all samples, both on the shoreline and at 500 m from the coast. the mean values of the three bacterial counts reflected the proximity of the terrestrial effluents. there was a positive correlation between aeromonads and faecal indicators on the shoreline but not at 500 m offshore. this reflected their common origin an ...19902246147
posttraumatic aeromonas hydrophila osteomyelitis. 19902251235
circulation in italy of beta-lactamase-producing strains within the major groups of bacterial pathogens.a multicenter study was undertaken in italy to assess the circulation of beta-lactamase-producing organisms and their current incidence within the major groups of bacterial pathogens. almost four thousand strains, all freshly isolated from clinical material, were examined at four centers serving different areas of italy. despite some significant center-to-center differences, this survey documented the occurrence of a large overall circulation of beta-lactamase-producing organisms among clinical ...19902253734
detection of the aerolysin gene in aeromonas hydrophila by the polymerase chain reaction.synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) technique to detect the gene for aerolysin in strains of aeromonas hydrophila and to screen for identical genes in a. caviae, a. sobria, and a. veronii isolated from patients with diarrheal disease. primers targeted a 209-bp fragment of the aer gene coding for the beta-hemolysin and detected template dna only in the pcr using nucleic acid (na) from hemolytic strains of a. hydrophila which were also cytotoxic to vero ...19902254423
bacterial production of histamine in some tropical fish.quantitative and qualitative distribution of histamine-forming bacteria associated with the fish rastrelliger kanagurta, sardinella longiceps, sillago sihama and liza subviridis, were investigated. these bacteria constituted a significant portion of the total bacterial population of fish and the values obtained in the present study were higher than those previously reported. the order of quantitative abundance of histamine-forming bacteria in the fish examined was: s. longiceps greater than r. k ...19902259288
the presence of aeromonas in drinking water supplies in the netherlands.the occurrence of aeromonas in raw, treated and distributed waters of 20 different treatment stations in the netherlands was studied over a one-and-a-half year period. river water yielded highest numbers (greater than 10,000 cfu/100 ml) of predominantly anaerogenic strains. in open storage reservoirs for river water or dune infiltrate, numbers were usually between 1,000 and 10,000/100 ml with a majority of aerogenic strains, in particular a. sobria. river water after dune infiltration or pure du ...19902261055
purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis.a fatty acyltransferase with a reaction mechanism similar to that of mammalian lecithin: cholesterol acyltransferase has been purified from culture supernatants of a mutant aeromonas salmonicida containing the cloned aeromonas hydrophila structural gene. typically, more than 35 mg of protein were isolated from 2 l of culture supernatant. the amino-terminal sequence, amino acid composition, and molecular weight of the purified protein corresponded to predictions based on the sequence of the gene, ...19902271578
antimicrobial susceptibility pattern of aeromonas and plesiomonas strains isolated from patients with diarrhoea in nigeria.the susceptibilities of 53 isolates of aeromonas species and 16 plesiomonas shigelloides, from patients with diarrhoea, to 15 commonly used antimicrobial agents were determined by the disk-agar diffusion technique. all the strains were susceptible to ofloxacin, a recent quinolone. also, over 70 pc of our strains were susceptible to gentamicin, carbenicillin, ceftriaxone, erythromycin, chloramphenicol, tetracycline and co-trimoxazole. however, less than 30 pc of our strains were susceptible to am ...19902272086
direct selection of monoclonal antibodies neutralising the cytotoxic activity of aeromonas sobria.monoclonal antibodies directed against the cytotoxic activity of aeromonas sobria were raised by immunising mice with a culture supernatant concentrated by ammonium sulphate precipitation. neutralising antibodies were specifically selected for by exposing hybridomas to cytotoxic levels of the immunising preparation. cultures free from cytopathic effects after three hours were selected for further investigation. ten cytotoxin resistant hybridomas were isolated but only two of these produced detec ...19902272497
usda expands early warning system for harmful food bacteria. 19902272871
in vitro activity of hre 664, a penem antibiotic.hre 664, a new penem antibiotic, inhibited 90% of escherichia coli, klebsiella, citrobacter diversus, proteus mirabilis, proteus vulgaris, salmonella, shigella, providencia, aeromonas, and morganella at less than or equal to 2 micrograms/ml but was considerably less active than cefotaxime, ceftazidime, and imipenem. it did not inhibit pseudomonas aeruginosa (mic greater than 128 micrograms/ml). hre 664 inhibited enterobacter spp., citrobacter freundii, and serratia marcescens at 1-8 micrograms/m ...19902279381
in vitro activities of 4-quinolones against the fish pathogen aeromonas salmonicida.the activities of five fluorinated 4-quinolones, namely, sarafloxacin, enrofloxacin, pd127391, pd117596, and ci934, against the fish pathogen aeromonas salmonicida were investigated and compared with that of oxolinic acid. the results indicated that with the exception of ci934, these drugs are more active than oxolinic acid in terms of mic. no inoculum effect was observed, but the drugs were less active at 10 degrees c than at 22 degrees c. the presence of 3% of nacl caused an increase in drug a ...19902285298
cloned aerolysin of aeromonas hydrophila is exported by a wild-type marine vibrio strain but remains periplasmic in pleiotropic export mutants.with a wide host range vector, the structural gene aera for the hole-forming extracellular protein aerolysin of aeromonas hydrophila was cloned into the marine vibrio sp. strain 60 and into three pleiotropic export mutants (epr mutants). the parent strain and all of the mutants were able to express the protein with the aera promoter in the plasmid. the parent strain exported proaerolysin into the medium, while all of the mutants accumulated the protoxin in their periplasms. two of the mutants al ...19902294091
structural and chemical characterization of the s layer of a pseudomonas-like bacterium.sections and freeze-fractured preparations showed an s layer on the surface of pseudomonas-like strain eu2. polyacrylamide gel electrophoresis of cell envelopes extracted with 1% sodium dodecyl sulfate (sds) at room temperature showed three proteins (45k, 55k, and 110k). the 55k protein was identified as the s-layer protein. incubation in 1.5 m guanidine hydrochloride removed the s layer from cell envelopes and dissociated the structure into subunits. the soluble 55k protein reassembled into pla ...19902298701
in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden.the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ...19902302351
in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response.the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ...19902311567
biochemical identification of aeromonas genospecies isolated from humans.one hundred phenotypic characteristics were determined for 138 clinical and environmental aeromonas strains. cluster analysis revealed three major phenons equivalent to the a. hydrophila, a. caviae, and a. sobria groups, each of which contained more than one genospecies and more than one named species. an excellent correlation was found between phenotypic identification and classification based on dna relatedness. dna hybridization groups within each of the phenotypic groups were also separable ...19902312673
pathogenicity factors and virulence for rainbow trout (salmo gairdneri) of motile aeromonas spp. isolated from a river.ninety-seven motile aeromonas strains were isolated over a period of a year from samples of water and sediment collected at different sites along a river. strains were regularly recovered from all samples, regardless of the source of isolation or seasonal conditions. isolates were biochemically characterized by the api 20ne system (analytab products, plainview, n.y.) and classified as aeromonas hydrophila (74 strains), aeromonas sobria (11 strains), and aeromonas caviae (12 strains). despite the ...19902312678
pyrazinamidase activity as a phenotypic marker for several aeromonas spp. isolated from clinical specimens.negative pyrazinamidase activity was significantly associated with aeromonas sobria, and positive pyrazinamidase activity was associated with a. hydrophila and a. caviae (chi 2, p less than 0.0001). the absence of pyrazinamidase activity may be a potentially significant phenotypic marker for a. sobria.19902312687
[in vitro sensitivity of aeromonas spp. to antibiotics].aeromonas strains, isolated from stool samples and aquatic biotopes were tested for their susceptibility to 20 antibiotics. the beta-lactamase-resistant cephalosporins, imipenem, aminoglycosides, ciprofloxacin, tetracycline, chloramphenicol and sulfamethoxazole + trimethoprim are highly efficient.19902316226
aeromonas associated diarrhoea in an otherwise healthy 70-year-old man. 19902319150
soft-tissue infection caused by edwardsiella tarda and aeromonas hydrophila. 19902319177
human serum antibody response to the presence of aeromonas spp. in the intestinal tract.a bacterial agglutination assay, a toxin-neutralizing assay, and an enzyme-linked immunosorbent assay (elisa) were used to compare antibodies against intestinal aeromonas strains in serum samples from healthy carriers (n = 6), from patients with acute (n = 15) or chronic (n = 8) gastroenteritis, from patients with gastroenteritis caused by other enteropathogenic bacteria (n = 3), and from healthy blood donors (n = 50). evaluation of the bacterial agglutination assay showed that it was not very u ...19902324279
aeromonas in adult diarrhea: an enteropathogen or an innocent bystander?this is a study from a district hospital in israel serving a population of 250,000. during 1986-1987, 1,005 stool specimens were cultured from 932 adult inpatients with acute diarrhea. seventeen yielded aeromonas species. of the 12 strains phenotyped, 10 qualified as enteropathogenic. five hundred stool specimens from asymptomatic control subjects, inhabitants of the same region, were negative for aeromonas. the finding of aeromonas as the apparently sole pathogen in acute adult diarrhea in a ge ...19902324479
diverse potential of beta-lactamase inhibitors to induce class i enzymes.the ability of various beta-lactamase inhibitors to induce class i beta-lactamases was assessed. clavulanate was the most active compound, inducing morganella morganii, aeromonas caviae, and enterobacter aerogenes over a broad concentration range and citrobacter freundii, pseudomonas aeruginosa, and serratia marcescens at high concentrations. disk approximation tests paralleled these results, with clavulanate, but not sulbactam or tazobactam, antagonizing the activity of several beta-lactams aga ...19902327752
purification and characterization of inducible beta-lactamases in aeromonas spp.beta-lactamases from aeromonas hydrophila and a. sobria were purified and characterized. both species produced beta-lactamases that were inducible by either cefoxitin or imipenem. these species were resistant to ampicillin and cephalothin but not imipenem. isoelectric focusing of sonic extracts revealed one band at pi 8.0 and a second band at pi 7.0 for a. hydrophila. likewise, a. sobria produced two bands, one at pi 8.4 and the other at pi 7.0. two enzymes from each species were separated by fl ...19902327760
in vitro activities of tosufloxacin, temafloxacin, and a-56620 against pathogens of diarrhea.tosufloxacin (a-60969 hcl), a new quinolone with broad activity against gram-positive and anaerobic organisms, was compared in vitro with other quinolones against bacterial pathogens of diarrhea. tosufloxacin was the most active agent against salmonella spp., shigella spp., campylobacter spp., aeromonas hydrophila, and vibrio spp. temafloxacin (a-62254) also demonstrated good activity against these organisms.19902327784
crystallization of a proform of aerolysin, a hole-forming toxin from aeromonas hydrophila.crystals of proaerolysin, the precursor of the hole-forming toxin from aeromonas hydrophila, have been obtained. the mature form of this protein binds to a receptor on mammalian cells, aggregates and forms 30 a holes in the membrane. the crystals are tetragonal, space group p4(3)2(1)2, a = b = 104.00 a, c = 222.0 a. they contain a dimer in the asymmetric unit and diffract to a resolution of 2.6 a.19902329576
antibacterial activity of eisenia fetida andrei coelomic fluid: transcription and translation regulation of lysozyme and proteins evidenced after bacterial infestation.1. after bacterial infestation lysozyme and antibacterial activities are enhanced, peaking at 4 hr and 3 days, respectively. 2. both humoral defenses require rna and protein de novo synthesis in response to pathogenic bacteria injection (actinomycin d and cycloheximide experiments). 3. antibacterial activity exists naturally at some basic level, involving regular translation of stable rnas. 4. when antibacterial activity reaches its maximum after bacterial injection, proteins responsible for it ...19902331874
evidence against dormancy in the bacterial fish pathogen aeromonas salmonicida subsp. salmonicida.results indicate that a. salmonicida does not enter an unculturable dormant state. the resuscitation of dormant cells by nutrient broth described by previous workers appears to be due to the presence of small numbers of viable, culturable cells too few to be detected by the sampling protocol employed.19902332152
a medium for the selective isolation of edwardsiella ictaluri.a selective medium, called edwardsiella ictaluri medium (eim), has been formulated for the isolation of edwardsiella ictaluri. the medium inhibits the growth of most gram-negative bacteria, except proteus sp., serratia marcescens and some isolates of aeromonas hydrophila and yersinia ruckeri. the bacteria that grow on the eim are easily differentiated from e. ictaluri based on colony morphology. the eim inhibits gram-positive bacteria with the exception of enterococci. the addition of fungizone ...19902338726
isolation and characterization of bacteriophage pm2 from aeromonas hydrophila.pm2 is an aeromonas-specific bacteriophage isolated on a. hydrophila strain ah-3. the bacteriophage receptor for this phage was found to be the lipopolysaccharide (lps), specifically a low-molecular weight lps fraction (lps-core oligosaccharides). mutants resistant to this phage were isolated and found to be devoid of lps o-antigen and altered in the lps-core. no other outer-membrane (om) molecules appeared to be involved in phage binding.19902341024
diarrhea and intestinal invasiveness of aeromonas strains in the removable intestinal tie rabbit model.twelve aeromonas strains were tested for virulence by using the removable intestinal tie adult rabbit diarrhea model. mortality was 50% or greater for 7 of 12 strains; 23 of 37 rabbits that died developed diarrhea before death, and 11 of 27 surviving rabbits developed diarrhea. aeromonas bacteremia was detected in 36 of 37 (97%) animals that died, but only in 2 of 27 (7%) survivors. death, diarrhea, and bacteremia were all strongly strain dependent. gastrointestinal lesions varied from moderate ...19902341185
observations on aeromonas species isolated from human faeces. 19902341740
occurrence of aeromonas species and plesiomonas shigelloides in patients with and without diarrhoea in lagos, nigeria.the prevalence of aeromonas spp. and plesiomonas shigelloides was determined in patients attending the enteric laboratory of the department of medical microbiology and parasitology, lagos university teaching hospital, nigeria. during the 12-month study (october 1986-september 1987), aeromonas spp. were isolated from 53 (2.26%) of 2350 patients with diarrhoea and only 2 (0.4%) of 500 patients without diarrhoea (p less than 0.01). similarly, p. shigelloides was isolated from 16 (0.68%) patients wi ...19902342086
an analysis of molecular polymorphism of esterase m produced by motile aeromonas strains.the high levels of electrophoretic polymorphism of esterase m detected in eight distinct hybridization groups of motile aeromonas raise questions of genetic homogeneity of the electromorphs. the 40 electromorphs detected fall in four mr classes--75, 80, 90, and 110 kd--and one typical variant belonging to each of these classes was purified. the four purified esterases exhibited the same resistance to heat, to ph and to diisopropyl fluorophosphate, the same km values for 1-naphthyl acetate and 1- ...19902344349
a survey of the microflora of raw and pasteurized milk and the sources of contamination in a milk processing plant in addis ababa, ethiopia.the microorganisms present in raw and pasteurized milk and the sources of contamination in the milk after it had arrived at the processing plant in addis ababa were studied. the lowest count registered for raw milk samples was 4 x 10(7) cfu/ml while the highest was 1 x 10(9) cfu/ml. pasteurized milk had mesophilic aerobic counts of 7 x 10(5) cfu/ml as it left the pasteurizing unit, but the population increased 2- to 4-fold as a result of subsequent contamination. of the total counts in raw milk, ...19902345191
clinical features of aeromonas enteritis in hawaii.the incidence and clinical features of enteritis associated with aeromonas in honolulu were studied. the stool isolation rate was 2.9% for aeromonas, the third most common bacterial pathogen in this population. clinical features of aeromonas-associated enteritis were compared with clinical features of enteritis without an associated bacterial pathogen. although there was a trend toward more abdominal pain, tenderness, nausea and prolonged illness in patients with aeromonas, these differences wer ...19902345913
a case of septicaemia due to aeromonas hydrophila.a case of aeromonas hydrophila septicaemia is reported, arising from the use of leeches in an attempt to salvage a replanted arm. it is believed that this is the first report of this complication.19902350649
aeromonas sobria in bubaline (water buffalo) abortion: growth requirement, biochemical characters, antibiotic susceptibility, experimental pathogenicity and serology.a. sobria was isolated unexpectedly on campylobacter selective medium from abortion specimens of 16 buffaloes. all strains (as1-as16) required campylobacter growth supplement (sodium pyruvate, sodium metabisulphate and ferrous sulphate) and 10% c02 atmosphere on primary isolation. they were unusually sensitive to bile salt and failed to grow on macconkey's agar. biochemically, these strains were homogeneous but antibiogram profile and physiological/other characters revealed a moderate heterogene ...19902351419
a note on aeromonas spp. from chickens as possible food-borne pathogens.the possible role of aeromonas spp. as potential food-borne psychrotrophic pathogens was investigated by examining organisms isolated from processed raw chicken for their biochemical characteristics, ability to produce exotoxins and to grow at chill temperatures. these strains, in particular a. sobria, with identical characteristics to human diarrhoea-associated aeromonads were readily found. chicken, and human and environmental (water) strains characterized in a previous study, were investigate ...19902351618
microbiologic and clinical evidence supporting the role of aeromonas caviae as a pediatric enteric pathogen.aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were isolated once each. the infants from whom a. caviae was isolated all presented with a watery diarrhea lasting 1 to 3 weeks. none of these infants was breast-fed, and all had a stool ph of greater than 7.5. all of the a. caviae isolates, including a reference strain (at ...19902351730
epidemiology of bacterial pathogens associated with infectious diarrhea in djibouti.during a survey examining the causes of diarrhea in the east african country of djibouti, 140 bacterial pathogens were recovered from 209 diarrheal and 100 control stools. the following pathogens were isolated at comparable frequencies from both diarrheal and control stools: enteroadherent escherichia coli (eaec) (10.6 versus 13%), enterotoxigenic e. coli (etec) (11 versus 10%), enteropathogenic e. coli (epec) (7.7 versus 12%), salmonella spp. (2.9 versus 3%), and campylobacter jejuni-c. coli (3 ...19902351738
extended serogrouping scheme for motile, mesophilic aeromonas species.a total of 1,255 strains of motile, mesophilic aeromonas species isolated from clinical and environmental specimens in the united kingdom and 258 strains isolated in australia, brazil, peru, and the united states were examined by using antisera for serogroups o1 to o44 (r. sakazaki and t. shimada, jpn. j. med. sci. 37:247-255, 1984) and for unpublished serogroup o45 (r. sakazaki). the typeability rate for strains isolated in the united kingdom was 35%; the strains isolated in other countries had ...19902351741
sensitivities of aeromonas hydrophila cultured from medicinal leeches to oral antibiotics.infections associated with medicinal leech application are caused by aeromonas hydrophila, an organism that resides in the leech gut. we cultured the intestinal tracts of 25 leeches and evaluated the efficacy of oral antibiotics against aeromonas hydrophila. cultures of this organism showed no sensitivity to ampicillin and inconsistent sensitivity to cephalothin (equivalent to cephalexin). the aeromonas hydrophila cultures did show consistent sensitivity to ciprofloxacin, tetracycline, and trime ...19902352221
isolation of aeromonas spp. from canal water.aeromonas sobria was identified from soil and canal water at the east end of london. there was a correlation between water and faecal isolates from children attending the nearby children's hospital. in view of the potential risk of enteric and non-enteric infections due to aeromonas spp, efforts are needed to ensure that canals, rivers, natural water sources and domestic water supply are free of these organisms.19902361702
bacterial aetiology of infantile diarrhoea in papua new guinea.bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ...19902362308
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