Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 3. inhibition of the enzyme]. | the alanine aminopeptidase from acinetobacter calcoaceticus is inhibited by sh-reagents like p-hydroxymercuribenzote, ellman's reagent, n-bromosuccinimide, and metal chelating agents like 1,10-phenanthroline. the aap is competitively inhibited by l-amino acids such as leucine, phenylalanine, and valine having hydrophobic side chains. bacitracin (ki = 2.0.10(-6) mol/l) inhibits aap stronger than puromycin (ki = 8.0.10(-6) mol/l). in contrast, the aeromonas aminopeptidase (ec 3.4.11.10) is stronge ... | 1990 | 1980190 |
| pili of an aeromonas hydrophila strain as a possible colonization factor. | aeromonas hydrophila ae6 had 2 morphologically distinctive kinds of pili. one appeared rigid and straight with a diameter of 9 nm (r-pili). the other appeared wavy and flexible with a diameter of 7 nm (w-pili). w-pili were very few on the cell as compared with r-pili. in this study, w-pili were purified and characterized. the pili consisted of a subunit protein with a molecular weight of 21 kda as estimated by sds-page. there was no immunological cross-reaction between w-pili and other cellular ... | 1990 | 1982552 |
| bacterial production of histamine in some tropical fish. | quantitative and qualitative distribution of histamine-forming bacteria associated with the fish rastrelliger kanagurta, sardinella longiceps, sillago sihama and liza subviridis, were investigated. these bacteria constituted a significant portion of the total bacterial population of fish and the values obtained in the present study were higher than those previously reported. the order of quantitative abundance of histamine-forming bacteria in the fish examined was: s. longiceps greater than r. k ... | 1990 | 2259288 |
| human serum antibody response to the presence of aeromonas spp. in the intestinal tract. | a bacterial agglutination assay, a toxin-neutralizing assay, and an enzyme-linked immunosorbent assay (elisa) were used to compare antibodies against intestinal aeromonas strains in serum samples from healthy carriers (n = 6), from patients with acute (n = 15) or chronic (n = 8) gastroenteritis, from patients with gastroenteritis caused by other enteropathogenic bacteria (n = 3), and from healthy blood donors (n = 50). evaluation of the bacterial agglutination assay showed that it was not very u ... | 1990 | 2324279 |
| the effect of terrestrial effluents on the incidence of aeromonas spp. in coastal waters. | the numbers of aeromonas species were monitored for one year at 18 stations on the barcelona coast. their concentrations exceed those of faecal coliforms and faecal streptococci in all samples, both on the shoreline and at 500 m from the coast. the mean values of the three bacterial counts reflected the proximity of the terrestrial effluents. there was a positive correlation between aeromonads and faecal indicators on the shoreline but not at 500 m offshore. this reflected their common origin an ... | 1990 | 2246147 |
| water associated infections. | 1990 | 2216140 | |
| [resistance to antimicrobial agents, hemolytic activity and plasmids in aeromonas species]. | a total of 174 aeromonas isolates consisting of 100 strains from patients with diarrhea being mainly overseas travellers nd healthy subjects, and 74 strains from environmental sources including foods, fish, fresh water, sea water and river soil collected in the area of tokyo metropolis and kanagawa prefecture was examined for the antimicrobial resistance, presence of plasmids and hemolytic activity. almost all the isolates (99.4%) were resistant to aminobenzyl penicillin. the isolation frequency ... | 1990 | 2401817 |
| action pattern of aeromonas hydrophila chitinase on partially n-acetylated chitosan. | oligosaccharides from the digestion of 34% n-acetylated chitosan by aeromonas hydrophila chitinase were separated by cm-sephadex c-25 column chromatography. sugar compositions and the sequences of main oligosaccharides were identified through their n-acetylation, their cleavage with exo-glycosidases, and their degradation with nitrous acid. hetero-chitooligosaccharides such as glcn.glcnac, glcn.glcnac.glcnac, glcnac.glcn.glcnac, and glcnac.glcn.glcnac.glcnac, together with glcnac and (glcnac)2, ... | 1990 | 1368549 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| aerolysin, a hemolysin from aeromonas hydrophila, forms voltage-gated channels in planar lipid bilayers. | the cytolytic toxin aerolysin was found to form ion channels which displayed slight anion selectivity in planar lipid bilayers. in voltage-clamp experiments the ion current flowing through the channels was homogeneous indicating a defined conformation and a uniform size. the channels remained open between -70 to +70 mv, but outside this range they underwent voltage-dependent inactivation which was observed as open-closed fluctuations at the single-channel level. zinc ions not only prevented the ... | 1990 | 1692344 |
| identification of the cell surface receptor for bacteriophage 18 from aeromonas hydrophila. | bacteriophage 18 was previously isolated by multiplication in aeromonas hydrophila. the bacteriophage receptor was shown to be the lipopolysaccharide (lps), specifically the low mw polysaccharide fraction (lps core oligosaccharide) a. hydrophila mutants, resistant to this phage were isolated and found to be devoid of lps o antigen by several criteria and had alterations in the lipopolysaccharide core. | 1990 | 1693217 |
| aerolysin of aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of staphylococcus aureus. | aerolysin from aeromonas sobria ab3 was isolated and purified. the pure toxin formed sodium dodecyl sulfate-insoluble oligomers in a lipidic environment. the addition of aerolysin to the aqueous phase bathing lipid bilayer membranes resulted in the formation of ion-permeable channels which had a single-channel conductance of about 70 ps in 0.1 m kcl. this defines the toxin as a channel-forming component similar to other toxins but without any indication for an association-dissociation reaction, ... | 1990 | 1694819 |
| pili of aeromonas hydrophila: purification, characterization, and biological role. | aeromonas hydrophila (ae6) has 2 morphologically distinctive kinds of pili. one appeared rigid, channeled, and straight with a diameter of 9 nm (ae6-r pili). the other looked flexible, wavy, and having helical structure with a diameter of 7 nm (ae6-w pili). ae6-r pili were purified and characterized. the pili consisted of a subunit protein with a molecular weight of 18 kda as estimated by sds-page, and contained 42.3% hydrophobic amino acids and one cysteine residue. the pilus was solubilized to ... | 1990 | 1971705 |
| the pili of aeromonas hydrophila: identification of an environmentally regulated "mini pilin". | ultrastructural studies of aeromonas hydrophila strain ah26 revealed two distinctive pilus types: "straight" pili appear as brittle, rod-like filaments, whereas "flexible" pili are supple and curvilinear. straight pili are produced constitutively under all tested conditions of growth. in contrast, the expression of flexible pili is regulated by physical and chemical variables, being produced at 22 vs. 37 degrees c, in a liquid vs. a solid medium, and when the availability of free-iron is reduced ... | 1990 | 1974915 |
| [pulmonary infection by aeromonas hydrophila following drowning in a swimming pool]. | 1990 | 2073626 | |
| analysis of the autoagglutination phenomenon, serogroup and hydrophobicity as virulence markers in mesophilic aeromonads isolated from stool cultures. | the autoagglutination phenomenon (aa) was studied in 71 strains of mesophilic aeromonads isolated from stool cultures of patients with acute diarrhea (28 aeromonas hydrophila, 20 aeromonas sobria, and 23 aeromonas caviae). two phenotypes were considered in the aa+ strains: sp+ (self-pelleting) and pab+ (precipitation after boiling). serogrouping of the strains was performed only with the 0:11 serum. other properties studied were agglutination with acriflavine (acr) and crystal violet uptake (cvb ... | 1990 | 2073902 |
| aeromonas hydrophila blepharoconjunctivitis. | 1990 | 2073904 | |
| ecology of mesophilic aeromonas spp. in aquatic environments of a temperate region and relationship with some biotic and abiotic environmental parameters. | the ecology of mesophilic aeromonas species has been investigated since january 1988 to examine their occurrence and distribution in aquatic environments of okayama prefecture. water and plankton samples were quantitatively as well as qualitatively analyzed throughout the seasons from five selected stations including fresh, brackish, and saline environments. analysis of variance and correlation coefficients among the biotic and abiotic parameters were sought. the organisms were found in all the ... | 1990 | 2080966 |
| [reactions of different enterobacteriaceae and vibrionaceae species in brila-mug (fluorocult) bouillion]. | in order to test the usefulness of brila-mug (= fluorocult) medium (merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the ec guidelines for bathing waters a total of 969 strains of different enterobacteriaceae and vibrionaceae species was examined under different culture conditions. these included 486 e. coli (reference strains of o-groups 1-170, enterotoxin and verotoxin-producing strains), 149 salmonella (subspecies i-vi), 92 yersinia ... | 1990 | 2080970 |
| cross resistance between oxytetracycline and oxolinic acid in aeromonas salmonicida associated with alterations in outer membrane proteins. | oxytetracycline resistant mutants of aeromonas salmonicida isolated from mutation frequency experiments showed decreased susceptibility to oxolinic acid. outer membrane preparations of these resistant mutant strains revealed a major protein, with a molecular mass of approximately 37 kda, which was not present in significant quantities in the parent strain. | 1990 | 2083844 |
| effect of bacterial pyrogen on three lizard species. | 1. three lizard species (callopistes maculatus, gerrhosaurus major, and varanus exanthematicus) were tested for their response to intraperitoneal injection of alcohol-killed aeromonas sobria. 2. a paired experimental design, in which each animal received an injection of sterile saline and 1 x 10(10) a. sobria, was utilized. 3. c. maculatus demonstrated a statistically significant increase in mean selected body temperature (msbt) after bacteria injection. 4. g. major and v. exanthematicus did not ... | 1990 | 1976479 |
| [new enteropathogens: aeromonas spp]. | aeromonas is one of the type of enteric pathogens which is commonly present in less developed countries. it leads to acute diarrhea in children and traveller's diarrhea in adults. additionally it is also responsible from the extraintestinal infections. aeromonas hydrophila is a bacteria which is isolated from water, soil, foods and rarely from intestinal tract of human. aeromonas sobria is especially isolated from wound infection of scuba diver's. there are other types of aeromonas such as salmo ... | 1990 | 2089235 |
| [analysis of the sensitivity of mesophilic aeromonas isolated from feces to antibiotics of everyday use]. | the sensitivity to antibiotics of 56 mesophilic strains of aeromonas (20 a. caviae, 18 a. sobria and 18 a. hydrophila) isolated from the feces of patients with enteritis, 89.2% of strains had some type of resistance, such as 83.9% to ampicillin, 32.1% to carbenicillin, 28.8% to cefalotin, 5.3% to co-trimoxazole and 1.7% to chloramphenicol. 48.2% of strains were simultaneously resistant to two of the tested antibiotics, 3.5% to three and 5.3% to four. 95.7% of ampicillin resistant strains were be ... | 1990 | 2094389 |
| [diarrhea caused by aeromonas in madrid]. | 1990 | 2095269 | |
| production of enterotoxin and cytotoxin by aeromonas veronii. | fourteen strains of aeromonas veronii were isolated from salt and fresh water in rio de janeiro and investigated for the presence of virulence factors. eleven (79%) a. veronii strains were positive for enterotoxin by the suckling-mouse test and thirteen (93%) produced hemolysin. of the 13 a. veronii strains that produced hemolysin, seven were investigated for cytotoxin production using vero cells as indicator cells and all of them were positive. | 1990 | 2095293 |
| wound infection due to fresh water contamination by aeromonas hydrophila. | we report the case of a 24-year-old man who sustained a scalp laceration in a diving accident. his wound became infected, and he was treated with an oral cephalosporin, with subsequent progression of the infection. cultures subsequently grew aeromonas hydrophila. many strains of aeromonas are not sensitive to the antibiotics commonly used for wound infections. aeromonas must be suspected as a pathogen in all wounds occurring in fresh water if proper treatment is to be initiated. | 1990 | 2096165 |
| [mesophile aeromonas gastroenteritis: value of biotyping as virulence marker]. | 1990 | 2098138 | |
| isolation of motile aeromonads from foods of animal origin. | the purpose of this work was to select an available medium for aeromonads isolation as well as to point out the aeromonads presence in different foodstuffs of animal origin. out of 15 tested media for aeromonas isolation, two media, were selected: dfs as control medium and agos medium, both giving superposable results. 404 samples of foodstuffs of animal origin were examined; aeromonads testing was performed qualitatively and quantitatively. 142 samples with a positivity percent of 51.40% were q ... | 1990 | 2101209 |
| etiological agents of diarrhoea. | two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status. | 1990 | 2101388 |
| aromatic-dependent mutants of aeromonas salmonicida. | 1990 | 2101485 | |
| detective work in continuous ambulatory peritoneal dialysis. | we report five cases of continuous ambulatory peritoneal dialysis in which the mechanisms and sources of infection were established. we show how diligent enquiry and environmental investigation can explain the pathogenesis of infection and help in prevention by motivation of the patient. | 1990 | 2108222 |
| aeromonas wound infections associated with outdoor activities--california. | 1990 | 2110619 | |
| effects of incorporating ampicillin, bile salts and carbohydrates in media on the recognition and selection of aeromonas spp. from faeces. | the effects of incorporating ampicillin, some bile salts and sugars into media on the primary recognition and selection of aeromonads from faeces were examined. most (88%) of the 101 aeromonas strains examined had an ampicillin mic of greater than or equal to 40 mg/l, and would be isolated on blood agar containing ampicillin 30 mg/l. the strains with an ampicillin mic of less than 40 mg/l were all of human origin and predominantly a. caviae. although ampicillin at 10, 20 or 30 mg/l in blood agar ... | 1990 | 2111407 |
| antimicrobial activity of ro 24-6778, a covalent bonding of desmethylfleroxacin and desacetylcefotaxime. | a new "dual action" cephalosporin (ro 24-6778), representing an ester-linked desacetylcefotaxime and desmethylfleroxacin was tested against 287 aerobic bacteria. ro 24-6778 was found to be very active (minimal inhibitory concentrations [mic90], less than or equal to 0.5 micrograms/ml) against enterobacteriaceae, streptococcus spp., and aeromonas hydrophila. moderate ro 24-6778 activity (mic90, 1-8 micrograms/ml) was demonstrated against bacillus spp., staphylococcus spp. (including oxacillin-res ... | 1990 | 2116952 |
| validation of flow cytometry for rapid enumeration of bacterial concentrations in pure cultures. | flow cytometry was investigated as a rapid detection and counting method for bacteria in pure cultures. a simple two-parameter detection scheme was employed: particle size was measured by forward angle light scatter and nucleic acid content by fluorescence of the dna/rna-binding dye ethidium bromide. the technique gave results that correlated exceptionally well with conventional plate counting for four species of bacteria, and concentrations in the range 10(2) to 10(7) cfu/ml. cytometric counts ... | 1990 | 2118900 |
| an investigation of indirect conductimetry for detection of some food-borne bacteria. | indirect conductimetry using a rapid automated bacterial impedance technique was investigated. strains of staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, aeromonas hydrophila and salmonella spp. grown in whitley impedance broth all elicited indirect conductimetric changes. these indirect conductance responses were improved by the addition of 2 g/l glucose to the medium and resulted in maximum changes of 2340-4300 ... | 1990 | 2126006 |
| in vitro activity of sparfloxacin (ci-978; at-4140) against clinical isolates from cancer patients. | the in vitro activity of sparfloxacin, a new quinolone, was compared with those of ciprofloxacin and fleroxacin against gram-positive and gram-negative bacteria, greater than 90% of which were isolated from blood culture specimens of cancer patients. sparfloxacin was extremely active against acinetobacter species, aeromonas hydrophila, citrobacter diversus, enterobacter species, escherichia coli, klebsiella species, proteus vulgaris, and serratia marcescens (inhibiting greater than 90% of these ... | 1990 | 2127348 |
| acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens. | the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ... | 1990 | 2127996 |
| plasmid screening amongst aeromonas species and plesiomonas shigelloides isolated from subjects with diarrhoea in lagos, nigeria. | fifty-three aeromonas strains and 16 plesiomonas shigelloides isolated from subjects with diarrhoea in lagos were screened for the presence of plasmids. nine (17%) of the aeromonas strains and one (6.3%) of the p. shigelloides harboured one or more plasmids, ranging in size from 2.4 to 16.8 mda. as has been documented in other enteropathogens, the possibilities are that these plasmids code for some factors to enhance the virulence of their hosts. | 1990 | 2128001 |
| a new and rapid method for the assay of bacterial fatty acids using high resolution capillary gas chromatography and trimethylsulfonium hydroxide. | gas-liquid chromatography of cellular fatty acids is a useful tool for the identification of bacteria. derivatization of bacterial fatty acids to methyl esters by conventional techniques is usually time-consuming and complicated. a new one-step technique using trimethyl-sulfonium hydroxide allows the direct formation of fatty acid methyl esters within 1-2 min. some random examples of profiles demonstrate that straight, branched, saturated, unsaturated, hydroxy and cyclopropyl fatty acids match c ... | 1990 | 2128179 |
| [mixed culture of enterococcus faecium sf 68 with pathogenic intestinal bacterial strains]. | an antagonistic activity of enterococcus faecium sf 68 towards plesiomonas shigelloides, aeromonas sp., enteropathogenic escherichia coli and yersinia enterocolitica has been studied and demonstrated. | 1990 | 2133323 |
| [infection of an open fracture caused by aeromonas hydrophila. a case report]. | with regard to a case report and the review of literature the authors point out that this uncommon but severe infection due to an aero-anaerobia bacteria, existing preferentially in stagnant or running water. this seriousness leads to the use of appropriate antibiotics instead of those usually prescribed in compound fractures, and most of the time, to the use of irrigation-stucking drainage of the wounds. | 1990 | 2142319 |
| [sensitivity of enteropathogenic bacteria to furazolidone]. | the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ... | 1990 | 2152485 |
| site-directed mutagenesis of the hole-forming toxin aerolysin: studies on the roles of histidines in receptor binding and oligomerization of the monomer. | the six histidines of the channel-forming protein aerolysin have been replaced one at a time with asparagine by site-directed mutagenesis, and each of the modified proteins has been purified. three proteins had the same hemolytic activity as native toxin, but the others, those changed at his107, his132, or his332, were less able to disrupt both human and rat erythrocytes. the largest reduction in activity, more than 100-fold, was observed with the his132 mutant protein. studies with radioiodinat ... | 1990 | 2158347 |
| restriction endonuclease fingerprinting analysis of canadian isolates of aeromonas salmonicida. | restriction endonuclease fingerprinting (ref) analysis was used to examine total cellular dna prepared from 56 independent field isolates of the fish pathogen, aeromonas salmonicida. dna was digested singly with the restriction enzymes ecori and hindiii, and the resulting fragments separated by polyacrylamide gel electrophoresis and visualized by silver staining. the ref patterns of typical isolates of a. salmonicida subsp. salmonicida were distinct from those of a. hydrophila, a. salmonicida su ... | 1990 | 2159364 |
| the in-vitro activity of piperacillin/tazobactam, ciprofloxacin, ceftazidime and imipenem against multiple resistant gram-negative bacteria. | one hundred and fifty gram-negative bacterial strains including respiratory pathogens, such as haemophilus influenzae and branhamella catarrhalis, and enterobacteriaceae with known resistance to beta-lactam and other antibiotics were tested in vitro for sensitivity to piperacillin, piperacillin/tazobactam (ratio 8:1), ceftazidime, ciprofloxacin and imipenem. a 16-fold or greater reduction in the mic90 of piperacillin was achieved by the addition of tazobactam, in the respiratory pathogens, thus ... | 1990 | 2164513 |
| glucose-mediated catabolite repression of the tricarboxylic acid cycle as an explanation for increased acetic acid production in suicidal aeromonas strains. | growth in the presence of glucose, even under highly aerobic conditions, significantly reduced the activities of three tricarboxylic acid cycle enzymes, citrate synthetase, alpha-ketoglutarate dehydrogenase, and malate dehydrogenase, in suicidal but not nonsuicidal aeromonas strains. pyruvate dehydrogenase activity, however, was significantly increased. the activities of all of the enzymes, as well as the glucose-mediated increase in acetic acid production, were shown to be regulated by cataboli ... | 1990 | 2165482 |
| particle agglutination assays to identify fibronectin and collagen cell surface receptors and lectins in aeromonas and vibrio species. | a rapid particle agglutination assay (paa) utilizing latex beads coated with connective tissue and serum proteins was evaluated for its ability to identify fibronectin, collagen (types i and iv), fibrinogen, and transferrin cell surface receptors on vibrio and aeromonas strains isolated from diseased fish, human infections, and the environment. similar tests were performed to screen for cell surface lectins. vibrio as well as aeromonas strains were found to bind connective tissue proteins (colla ... | 1990 | 2166473 |
| [clinical evaluation of fosfomycin]. | a total of 15 patients with a variety of infectious disease were treated with fosfomycin in chang gung memorial hospital, kaoshiung. the drug was administered by iv drip infusion in doses of 204g per day for 5-28 days. the clinical response was satisfactory in 6 (100%) of 6 patients with urinary tract infection, 8 (80%) of 10 with septicemia and 1 with pneumonia. overall, fosfomycin was effective in 13 (86%) of all patients treated. except for 1 isolate of the pathogen, b. fragilis, and another ... | 1990 | 2168262 |
| aeromonas hydrophila water-associated traumatic wound infections: a review. | the bacterium aeromonas hydrophila is commonly found in natural bodies of water, and is a potential invasive pathogen for those who suffer trauma while submerged in water. five patients treated by the authors and 18 previously reported patients were reviewed to further define the characteristics of a. hydrophila wound infections. although aeromonas has been isolated from salt and brackish water, all 23 cases occurred following contact with fresh water. signs of infection usually appeared 8-48 ho ... | 1990 | 2179571 |
| aeromonas species bacteremia in nonimmunocompromised hosts. two case reports and a review of the literature. | aeromonas hydrophila septicemia usually occurs in immunocompromised hosts. we present two case reports of aeromonas hydrophila septicemia in uncompromised patients from two medical centers. three additional cases (two in immunocompromised hosts and one in a normal host) were found in these centers after retrospective screening of bacteriologic records over the last 3 years. a review of the english medical literature over four decades revealed only 24 additional cases of aeromonas infection in th ... | 1990 | 2180851 |
| third-generation cephalosporins in the treatment of rare infections. | this article reviews the published clinical experience of the use of the third-generation cephalosporins in the treatment of rare infections. rare infections are defined as those caused by unusual pathogens or multi-resistant organisms as well as those occurring in unusual or pharmacologically protected body sites. examples of such infections are uncommon causes of meningitis and ventriculitis, brain abscess, rare causes of bacterial endocarditis, metastatic salmonella infections, spontaneous ba ... | 1990 | 2183606 |
| biochemical characteristics and a simple scheme for the identification of aeromonas species and plesiomonas shigelloides. | the biochemical characteristics of 55 strains of aeromonas spp. and 16 p. shigelloides of human faecal origin were examined. results of tests for the production of oxidase, acid from xylose, dulcitol, adonitol, mannitol, or inositol; greenish pigmentation on nutrient agar, and growth in 6.5% nacl broth were found to be specific and reproducible. however, other tests such as haemolysis on blood agar (ba), production of dnase, amylase, ornithine or lysine decarboxylase; citrate utilization and the ... | 1990 | 2190003 |
| purification of cloned proaerolysin released by a low protease mutant of aeromonas salmonicida. | the precursor to the hole-forming toxin aerolysin has been purified in high yield from culture supernatants of a mutant of aeromonas salmonicida containing the cloned structural gene. the mutant strain was generated by tn5 mutagenesis. it released little or no protease or other extracellular proteins, including phospholipase, suggesting that it is a regulatory mutant. the absence of protease allowed the isolation of protoxin free from contaminating aerolysin. typically, more than 50 mg of pure p ... | 1990 | 2190617 |
| [inhibition of enteropathogens by clostridium butyricum miyairi 588]. | the inhibitory effect of clostridium butyricum miyairi 588 against various enteropathogens was investigated in mixed cultures. it was observed that c. butyricum m588 inhibited the growth of vibrio cholerae o1, v. cholerae non-o1, aeromonas hydrophila, and shigella flexneri. considering that the interaction between c. butyricum and shigella is especially important because of their proliferation site in the lower intestine, further examinations were carried out on shigella in particular. results w ... | 1990 | 2193065 |
| molecular cloning and characterization of an extracellular protease gene from aeromonas hydrophila. | a structural gene which codes for an extracellular protease in aeromonas hydrophilia so2/2 and d13 was cloned in escherichia coli c600-1 by using pbr322 as a vector. the gene codes for a temperature-stable protease with a molecular mass of approximately 38,000 daltons. the protein was secreted to the periplasm of e. coli c600-1 and purified by osmotic shock. cloned protease (p3) was identical in molecular mass and properties to the one purified from a. hydrophila so2/2 culture supernatant as an ... | 1990 | 2193924 |
| acute gastroenteritis due to double infection with enteropathogenic escherichia coli or salmonella and another bacterial pathogen. | two enteric bacterial pathogens were concomitantly isolated from the feces of 18 infants less than 6 months of age admitted to the assaf harofeh medical center over a 7-year period. in all but two patients stool cultures grew enteropathogenic escherichia coli, six of serogroup 0119. the other organisms cultured were shigella, salmonella, campylobacter jejuni and aeromonas hydrophila. the usual clinical presentation was diarrhea, dehydration and vomiting of acute onset, and low grade fever. patie ... | 1990 | 2199408 |
| isolation and identification of autoagglutinating serogroup o:11 aeromonas strains in the clinical laboratory. | we evaluated the extent to which serogroup o:11 aeromonas strains could be recovered from both clinical and environmental specimens and the cultural parameters that affected the phenotypic marker (autoagglutination) associated with this group. of over 200 aeromonas strains screened, serogroup o:11 was identified only among the phenospecies a. hydrophila and a. sobria and was associated with clinical isolates more frequently than with environmental strains. blood and wound isolates accounted for ... | 1990 | 2199496 |
| production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, brazil. | an elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ... | 1990 | 2199511 |
| a concurrent increasing of natural antibodies and enhancement of resistance to furunculosis in rainbow trout. | in order to compare the respective roles of specific response and non-specific mechanisms in the enhancement of resistance to furunculosis, 2 groups of rainbow trout (oncorhynchus mykiss) were immunized with supernatants of aeromonas salmonicida or escherichia coli cultures. the fish received 4 weekly intraperitoneal injections (70 micrograms of crude proteins). one control group was injected with phosphate-buffered saline, while another was untreated. specific agglutinins were produced only in ... | 1990 | 2202250 |
| survival of aeromonas hydrophila and escherichia coli in aquatic environments. | survival of aeromonas hydrophila and escherichia coli in distilled water, pond water and effluents of anaerobic digesters were examined. survival capabilities of a. hydrophila in various aquatic systems were similar to e. coli except in the raw effluents of anaerobic digesters, where a. hydrophila survived significantly better than e. coli for 6 days. pathogens such as staphylococcus aureus, salmonella enteritidis and vibrio cholerae were readily killed in the raw effluents of the anaerobic dige ... | 1990 | 2203637 |
| virulence factors in motile aeromonas species. | 1990 | 2204614 | |
| [the occurrence of bacteria pathogenic for humans in freshwater fish]. | freshwater fish are of minor importance as vectors of food-borne disease in humans. these diseases can only rarely be traced back to bacterial pathogens. however, there is a number of bacteria with facultative pathogenicity for man, which are part of the natural aquatic environment. among these bacteria, the motile aeromonads (a. sp.) have become more and more important. a review is presented on a. sp. infections which have been traced back to contact with fish or water. critical discussion of t ... | 1990 | 2205462 |
| temperature as an environmental factor influencing the pathogenicity of aeromonas hydrophila. | contamination of wounds by surface water and the aspiration of such water following an accident can lead to severe septic infection by a. hydrophila. as such water is often found at low temperatures, the question should be asked whether this particular water environment influences the pathogenicity of this organism. some experiments have been undertaken to assess if the pathogenicity of a. hydrophila is affected by temperature. the pathogenicity of a. hydrophila adapted to the low temperature of ... | 1990 | 2206202 |
| mucosal intussusception to avoid ascending cholangitis. | many methods have been devised to prevent ascending cholangitis following kasai's hepatic portoenterostomy for biliary atresia. to investigate the effectiveness of mucosal intussusception in preventing ascending cholangitis, 20 mongrel dogs were randomized to undergo roux-en-y cholecystjejunostomy and common bile duct ligation with or without mucosal intussusception. aeromonas hydrophila was instilled into the gastrointestinal tract. bacterial cultures were grown from samples taken from the gall ... | 1990 | 2207590 |
| rapid presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity. | a new test combination for presumptive phenospecies identification of mesophilic aeromonads by testing for suicidal activity in conjunction with esculin hydrolysis and gas production from glucose was evaluated. of 40 clinical isolates of aeromonas tested statically at 30 degrees c, seven were identified as aeromonas hydrophilia (non-suicidal, aerogenic, esculin positive), eight as aeromonas sobria (suicide variable, mostly aerogenic, esculin negative), and 25 as aeromonas caviae (suicidal, anaer ... | 1990 | 2209629 |
| the incidence of virulence factors in mesophilic aeromonas species isolated from farm animals and their environment. | sixty-one isolates of aeromonas spp. from the faeces of pigs, cows and a variety of associated environmental sources were examined for the characteristics that are reputed to have roles in pathogenicity. most isolates of aeromonas hydrophila were cytotoxic (96.4%) and were capable of producing cell elongation factor (75%) and haemagglutinins (67.9%). in contrast few of the aeromonas caviae isolates produced these three markers (13.6%, 27.3% and 36.4% respectively). in general, aeromonas sobria o ... | 1990 | 2209733 |
| isolation and characterization of bacteriophage pm3 from aeromonas hydrophila the bacterial receptor for which is the monopolar flagellum. | pm3 is an aeromonas-specific bacteriophage which was isolated and characterized on a. hydrophila strain tf7. spontaneous mutants resistant to pm3 were non-motile having lost their characteristic monopolar flagellum. in addition, purified flagella inactivated pm3. pm3 is the first filamentous bacteriophage isolated on aeromonas, the adsorption site for which is the monopolar flagellum. | 1990 | 2210340 |
| comparative study of the prevalence and clinical profiles of diarrheas due to aeromonas and other enteric pathogens. | the prevalence of aeromonas spp. and other enteric pathogens in stool specimens from diarrheic and non-diarrheic patients was studied over a 12 month period (january to december, 1986). except for the absence of fever, all the clinical features in aeromonas diarrhea were comparable to those associated with other diarrheagenic agents. these features included abdominal pain (30%), vomiting (24.5%), fever (31.5%), dehydration (9.5%) and hematochezia (19.5%). aeromonas spp. were more frequently isol ... | 1990 | 2212640 |
| hemolysin as a marker for serratia. | all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ... | 1990 | 2222120 |
| characterization of aeromonas salmonicida variants with altered cell surfaces and their use in studying surface protein assembly. | aeromonas salmonicida variants were characterized for alterations in their cell surface structure and used to examine reconstitution of the surface protein layer (a-layer). variants lacking outer membrane o-polysaccharide were devoid of a-layer and excreted stainable floret-like material of the surface protein (a-protein). one variant, showing partial loss of o-polysaccharide, was associated with a disrupted a-layer and excretion of some a-protein. variants lacking a-protein but possessing o-pol ... | 1990 | 2222123 |
| comparative in vitro activity of cefpirome and cefepime, two new cephalosporins. | in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ... | 1990 | 2226498 |
| electrophoretic analysis of the surface components of autoagglutinating surface array protein-positive and surface array protein-negative aeromonas hydrophila and aeromonas sobria. | the protein and lipopolysaccharide (lps) compositions of 10 autoagglutinating aeromonas hydrophila and aeromonas sobria strains were studied; one group consisted of five serogroup o:11 strains that contained an s layer, while a second group was composed of diverse serogroups that were s layer negative by transmission electron microscopy. all serogroup o:11 strains were found to contain a predominant 52,000- to 54,000-molecular-weight protein that was present on both whole-cell and outer membrane ... | 1990 | 2229347 |
| aerobic bacteria cultured from the mouth of the american opossum (didelphis virginiana) with reference to bacteria associated with bite infections. | the american opossum inflicts bite injuries both when hunted for food and when accidentally provoked when handled in captivity. this study involved aerobically culturing organisms from the mouths of seven wild opossums (didelphis virginiana). isolates included streptococci, coagulase-positive and -negative staphylococci, aeromonas spp., citrobacter freundii, eikenella corrodens, and escherichia coli. | 1990 | 2229365 |
| [an unusual hospital infection: aeromonas hydrophila infection due to the use of leeches]. | a patient is described with an infection with aeromonas hydrophila after the use of medical leeches to improve the circulation of a congested region of surgery. the use of medical leeches and the treatment of a leech-related infection are discussed. | 1990 | 2234189 |
| very prolonged diarrhea associated with aeromonas hydrophila. | 1990 | 2239888 | |
| production of exotoxins by aeromonas spp. at 5 degrees c. | the ability of 60 strains of aeromonas to produce enterotoxin and haemolysin after cultivation at 5 degrees c for 7-10 d was investigated. the strains were isolated from lamb meat, offal, carcasses and faeces, and had previously been tested for their ability to produce these exotoxins at 37 degrees c. the results showed that some strains of aeromonas hydrophila and a. sobria were capable of producing enterotoxin and haemolysin at 5 degrees c, but none of the a. caviae strains tested produced the ... | 1990 | 2246140 |
| posttraumatic aeromonas hydrophila osteomyelitis. | 1990 | 2251235 | |
| circulation in italy of beta-lactamase-producing strains within the major groups of bacterial pathogens. | a multicenter study was undertaken in italy to assess the circulation of beta-lactamase-producing organisms and their current incidence within the major groups of bacterial pathogens. almost four thousand strains, all freshly isolated from clinical material, were examined at four centers serving different areas of italy. despite some significant center-to-center differences, this survey documented the occurrence of a large overall circulation of beta-lactamase-producing organisms among clinical ... | 1990 | 2253734 |
| detection of the aerolysin gene in aeromonas hydrophila by the polymerase chain reaction. | synthetic oligonucleotide primers were used in a polymerase chain reaction (pcr) technique to detect the gene for aerolysin in strains of aeromonas hydrophila and to screen for identical genes in a. caviae, a. sobria, and a. veronii isolated from patients with diarrheal disease. primers targeted a 209-bp fragment of the aer gene coding for the beta-hemolysin and detected template dna only in the pcr using nucleic acid (na) from hemolytic strains of a. hydrophila which were also cytotoxic to vero ... | 1990 | 2254423 |
| purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis. | a fatty acyltransferase with a reaction mechanism similar to that of mammalian lecithin: cholesterol acyltransferase has been purified from culture supernatants of a mutant aeromonas salmonicida containing the cloned aeromonas hydrophila structural gene. typically, more than 35 mg of protein were isolated from 2 l of culture supernatant. the amino-terminal sequence, amino acid composition, and molecular weight of the purified protein corresponded to predictions based on the sequence of the gene, ... | 1990 | 2271578 |
| direct selection of monoclonal antibodies neutralising the cytotoxic activity of aeromonas sobria. | monoclonal antibodies directed against the cytotoxic activity of aeromonas sobria were raised by immunising mice with a culture supernatant concentrated by ammonium sulphate precipitation. neutralising antibodies were specifically selected for by exposing hybridomas to cytotoxic levels of the immunising preparation. cultures free from cytopathic effects after three hours were selected for further investigation. ten cytotoxin resistant hybridomas were isolated but only two of these produced detec ... | 1990 | 2272497 |
| usda expands early warning system for harmful food bacteria. | 1990 | 2272871 | |
| in vitro activity of hre 664, a penem antibiotic. | hre 664, a new penem antibiotic, inhibited 90% of escherichia coli, klebsiella, citrobacter diversus, proteus mirabilis, proteus vulgaris, salmonella, shigella, providencia, aeromonas, and morganella at less than or equal to 2 micrograms/ml but was considerably less active than cefotaxime, ceftazidime, and imipenem. it did not inhibit pseudomonas aeruginosa (mic greater than 128 micrograms/ml). hre 664 inhibited enterobacter spp., citrobacter freundii, and serratia marcescens at 1-8 micrograms/m ... | 1990 | 2279381 |
| in vitro activities of 4-quinolones against the fish pathogen aeromonas salmonicida. | the activities of five fluorinated 4-quinolones, namely, sarafloxacin, enrofloxacin, pd127391, pd117596, and ci934, against the fish pathogen aeromonas salmonicida were investigated and compared with that of oxolinic acid. the results indicated that with the exception of ci934, these drugs are more active than oxolinic acid in terms of mic. no inoculum effect was observed, but the drugs were less active at 10 degrees c than at 22 degrees c. the presence of 3% of nacl caused an increase in drug a ... | 1990 | 2285298 |
| cloned aerolysin of aeromonas hydrophila is exported by a wild-type marine vibrio strain but remains periplasmic in pleiotropic export mutants. | with a wide host range vector, the structural gene aera for the hole-forming extracellular protein aerolysin of aeromonas hydrophila was cloned into the marine vibrio sp. strain 60 and into three pleiotropic export mutants (epr mutants). the parent strain and all of the mutants were able to express the protein with the aera promoter in the plasmid. the parent strain exported proaerolysin into the medium, while all of the mutants accumulated the protoxin in their periplasms. two of the mutants al ... | 1990 | 2294091 |
| structural and chemical characterization of the s layer of a pseudomonas-like bacterium. | sections and freeze-fractured preparations showed an s layer on the surface of pseudomonas-like strain eu2. polyacrylamide gel electrophoresis of cell envelopes extracted with 1% sodium dodecyl sulfate (sds) at room temperature showed three proteins (45k, 55k, and 110k). the 55k protein was identified as the s-layer protein. incubation in 1.5 m guanidine hydrochloride removed the s layer from cell envelopes and dissociated the structure into subunits. the soluble 55k protein reassembled into pla ... | 1990 | 2298701 |
| in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden. | the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ... | 1990 | 2302351 |
| in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response. | the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ... | 1990 | 2311567 |
| pathogenicity factors and virulence for rainbow trout (salmo gairdneri) of motile aeromonas spp. isolated from a river. | ninety-seven motile aeromonas strains were isolated over a period of a year from samples of water and sediment collected at different sites along a river. strains were regularly recovered from all samples, regardless of the source of isolation or seasonal conditions. isolates were biochemically characterized by the api 20ne system (analytab products, plainview, n.y.) and classified as aeromonas hydrophila (74 strains), aeromonas sobria (11 strains), and aeromonas caviae (12 strains). despite the ... | 1990 | 2312678 |
| aeromonas associated diarrhoea in an otherwise healthy 70-year-old man. | 1990 | 2319150 | |
| soft-tissue infection caused by edwardsiella tarda and aeromonas hydrophila. | 1990 | 2319177 | |
| diverse potential of beta-lactamase inhibitors to induce class i enzymes. | the ability of various beta-lactamase inhibitors to induce class i beta-lactamases was assessed. clavulanate was the most active compound, inducing morganella morganii, aeromonas caviae, and enterobacter aerogenes over a broad concentration range and citrobacter freundii, pseudomonas aeruginosa, and serratia marcescens at high concentrations. disk approximation tests paralleled these results, with clavulanate, but not sulbactam or tazobactam, antagonizing the activity of several beta-lactams aga ... | 1990 | 2327752 |
| purification and characterization of inducible beta-lactamases in aeromonas spp. | beta-lactamases from aeromonas hydrophila and a. sobria were purified and characterized. both species produced beta-lactamases that were inducible by either cefoxitin or imipenem. these species were resistant to ampicillin and cephalothin but not imipenem. isoelectric focusing of sonic extracts revealed one band at pi 8.0 and a second band at pi 7.0 for a. hydrophila. likewise, a. sobria produced two bands, one at pi 8.4 and the other at pi 7.0. two enzymes from each species were separated by fl ... | 1990 | 2327760 |
| in vitro activities of tosufloxacin, temafloxacin, and a-56620 against pathogens of diarrhea. | tosufloxacin (a-60969 hcl), a new quinolone with broad activity against gram-positive and anaerobic organisms, was compared in vitro with other quinolones against bacterial pathogens of diarrhea. tosufloxacin was the most active agent against salmonella spp., shigella spp., campylobacter spp., aeromonas hydrophila, and vibrio spp. temafloxacin (a-62254) also demonstrated good activity against these organisms. | 1990 | 2327784 |
| crystallization of a proform of aerolysin, a hole-forming toxin from aeromonas hydrophila. | crystals of proaerolysin, the precursor of the hole-forming toxin from aeromonas hydrophila, have been obtained. the mature form of this protein binds to a receptor on mammalian cells, aggregates and forms 30 a holes in the membrane. the crystals are tetragonal, space group p4(3)2(1)2, a = b = 104.00 a, c = 222.0 a. they contain a dimer in the asymmetric unit and diffract to a resolution of 2.6 a. | 1990 | 2329576 |
| evidence against dormancy in the bacterial fish pathogen aeromonas salmonicida subsp. salmonicida. | results indicate that a. salmonicida does not enter an unculturable dormant state. the resuscitation of dormant cells by nutrient broth described by previous workers appears to be due to the presence of small numbers of viable, culturable cells too few to be detected by the sampling protocol employed. | 1990 | 2332152 |
| a medium for the selective isolation of edwardsiella ictaluri. | a selective medium, called edwardsiella ictaluri medium (eim), has been formulated for the isolation of edwardsiella ictaluri. the medium inhibits the growth of most gram-negative bacteria, except proteus sp., serratia marcescens and some isolates of aeromonas hydrophila and yersinia ruckeri. the bacteria that grow on the eim are easily differentiated from e. ictaluri based on colony morphology. the eim inhibits gram-positive bacteria with the exception of enterococci. the addition of fungizone ... | 1990 | 2338726 |
| isolation and characterization of bacteriophage pm2 from aeromonas hydrophila. | pm2 is an aeromonas-specific bacteriophage isolated on a. hydrophila strain ah-3. the bacteriophage receptor for this phage was found to be the lipopolysaccharide (lps), specifically a low-molecular weight lps fraction (lps-core oligosaccharides). mutants resistant to this phage were isolated and found to be devoid of lps o-antigen and altered in the lps-core. no other outer-membrane (om) molecules appeared to be involved in phage binding. | 1990 | 2341024 |
| observations on aeromonas species isolated from human faeces. | 1990 | 2341740 |