Publications

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nature, characteristics, and proteolytic properties of beef spoilage bacteria at low and high temperatures. 19676049311
[demonstration of glutamic acid decarboxylase in bacteria using an automatic technic]. 19676059741
growth of marine bacteria on some organic acids and its application to the selective isolation of pseudomonads. 19676064045
numerical taxonomy of some named bacterial cultures. 19676064342
transmission of multiple drug-resistance from shigella to aeromonas and non-agglutinable vibrio through conjugation. 19676072423
the rate of evolutionary divergence of initiation factors if2 and if3 in various bacterial species determined quantitatively by immunoblotting.antibodies to escherichia coli translational initiation factors if2 and if3 were used for an immunological comparison of unpurified proteins from the following genera: salmonella, serratia, proteus, aeromonas, pseudomonas, streptococcus, sarcina and bacillus. immunological relatedness was compared by ouchterlony double diffusion experiments and immunoblotting analysis. immunoblotting is a quantitative technique for measuring levels of specific proteins in crude cell lysates. we have used this te ...19846084987
aeromonas hydrophila: an outbreak of hospital infection.aeromonas hydrophila is a gram-negative water-borne organism widely distributed in the environment. this organism is a recognized cause of diarrhoea and an opportunist pathogen in immunosuppressed patients. an outbreak of hospital-acquired infection which included three cases of pneumonia is reported. the organism was isolated from 19 patients.19846085098
pyogenic meningitis manifesting during therapy for aeromonas hydrophila sepsis.pyogenic meningitis became apparent on the third day of ampicillin and gentamicin therapy for aeromonas hydrophila sepsis in a patient with severe alcoholic hepatitis. the patient responded clinically to therapy with intravenous cefotaxime sodium and gentamicin sulfate. antibiotic therapy that provides adequate csf concentrations should be considered in the treatment of patients with aeromonas sepsis.19846091581
new medium for detection of esterase and gelatinase activity.a new medium was developed for detecting esterase and gelatinase activities in aerobic and facultatively anaerobic bacteria. the new medium was tested with various strains of bacteria and the results showed agreement between the reactions in the new medium and those obtained by conventional techniques. the new medium is more economical and may be used for a rapid differentiation of serratia, aeromonas and vibrio species from biochemically similar bacteria.19846098101
hemagglutination properties and adherence ability of aeromonas hydrophila.human diarrheal isolates and enterotoxigenic strains of aeromonas hydrophila are strong hemagglutinators with human blood cells. sugar inhibition studies and yeast coagglutination tests with 11 selected strains revealed six different hemagglutination mechanisms for this species. these were characterized by inhibition by l-fucose, inhibition by d-galactose, inhibition by d-mannose, and two distinguishable mechanisms which were inhibited by either l-fucose or d-mannose, one being pilus mediated. i ...19806103874
association of aeromonas sobria with human infection.fifteen aeromonas isolates from various human infections and nine isolates from polluted water were identified as either aeromonas hydrophila or aeromonas sobria and examined for cytotoxigenicity, enterotoxigenicity, adherence to epithelial cells, and other virulence-associated factors, including proteases, lipases, elastases, and hemolysins. two groups of organisms (i and ii) were distinguishable based on differences in median lethal doses in mice and cytotoxicity for y-1 adrenal cells. group i ...19816112237
aeromonas bacteraemia in a burn patient. 19816117752
clustering of aeromonas hydrophila septicaemia. 19816118660
aeromonas species as enteric pathogens. 19826119581
aeromonas hydrophila liver abscess. 19826121985
the use of aerolysin toxin as an aid for visualization of low numbers of african trypanosomes in whole blood.a method was developed for detection of low numbers of african trypanosomes in whole blood. the method is based on selective lysis of erythrocytes and leukocytes by aerolysin, a toxin produced by the bacterium aeromonas hydrophila. african trypanosomes do not bind the toxin and their viability and motility are therefore unaffected by this treatment.19826122365
enteropathogenicity: recent developments.recent studies have added important new information to our understanding of the pathogenesis and ethiology of diarrheal disease. vibrio cholerae produces a heat-labile enterotoxin, affecting cyclic amp. a very similar heat-labile enterotoxin is produced also by certain strains of escherichia coli, as well as by citrobacter, klebsiella, and aeromonas. e. coli may also produce a heat-stable enterotoxin, stimulating guanylate cyclase activity. in order to produce the pathologic effects, e. coli fir ...19826126611
aeromonas-associated gastroenteritis.enterotoxigenic aeromonas spp. were isolated from 118 (10.2%) children with diarrhoea and 7 (0.6%) of those without diarrhoea in a prospective, year-long study of 1156 children with gastroenteritis and the same number of age and sex matched controls. in perth, western australia, aeromonas-associated diarrhoea is distinctly seasonal with a sharp summer peak. the disease most commonly presents in children under two years of age as watery diarrhoea of short duration and mild fever which require no ...19826128598
isolation of aeromonas from faeces. 19836130348
haemagglutination properties & fimbriation in enterotoxigenic aeromonas hydrophila strains. 19836143724
interactions of erythrocytes with bacteria under shear. 19836145382
distribution and characterization of kepone-resistant bacteria in the aquatic environment.effects of the chlorinated insecticide kepone on the ecology of chesapeake bay and james river bacteria were studied. kepone-resistant bacteria present in a given environment were found to reflect the degree of fecal and/or high organic pollution of the sampling sites, based on total numbers and generic composition of the populations of kepone-resistant bacteria. the presence of kepone-resistant bacteria was found to be correlated (alpha = 0.01) with total coliforms, fecal coliforms, and total a ...19806155825
the effect of primary and secondary immunization on the lymphoid tissues of the carp, cyprinus carpio l.mirror carp immunized with human gamma globulin (hgg) in freund's complete adjuvant (fca) show a proliferative response involving cells whose cytoplasm stains deep red with methyl green-pyronin (pyroninophilic cells). this response occurs particularly in the haemopoietic parenchyma of the pronephros and mesonephros. it peaks at week 3, with the formation of clusters of pyroninophilic cells in the pronephros. immunization with aeromonas salmonicida elicited a less intense pyroninophilic response ...19826177828
structural studies on the o-antigen of aeromonas salmonicida.lipopolysaccharide from a strain of aeromonas salmonicida salmonicida was isolated from cells by the aqueous phenol method in 2.3% yield (based on dry weight of bacteria). hydrolysis of the lipopolysaccharide in 1% acetic acid afforded o-polysaccharide (19% by weight), core-oligosaccharide (12.2%) and lipid a (44.6%). analysis indicated that 3-deoxy-d-manno-2-octulosonic acid was absent from the lipopolysaccharide and that no low-molecular-weight compounds were released by the mild hydrolysis. t ...19836188611
patterns of intestinal infection in australian aboriginal children.a year-long, prospective study of aboriginal children with diarrhoea showed a high rate of isolation of bacterial pathogens, viruses and parasites in faecal specimens. seventeen per cent of patients had enterotoxigenic bacteria in their stools; none of the 132 aboriginal children without diarrhoea studied at the same time had enterotoxigenic bacteria isolated. salmonellae and shigellae were also much more common in the diarrhoea group. bacterial pathogens including enterotoxigenic e. coli and ae ...19836191647
in vitro susceptibility of hospital isolates of various bacterial genera to chlorhexidine. 19836195226
heterotrophic bacteria accompanying stichococcus bacillaris in laboratory cultures.the number and composition of bacterial microflora accompanying stichococcus bacillaris in various media with urea was determined. the number of heterotrophic bacteria during 5 days of incubation increased 10-fold. only some of the isolated bacterial strains were able to grow in medium for algae supplemented with dead s. bacillaris cells. it is suggested that bacteria utilize organic matter released to the medium by s. bacillaris. the most numerous among the isolated bacteria were enterobacteria ...19836198881
immunodominant protein epitopes. i. induction of suppression to hen egg white lysozyme is obliterated by removal of the first three n-terminal amino acids.the lack of response to hen egg white lysozyme (hel) by c57bl (h-2b) mice has been demonstrated previously to be related to the induction of suppressor t (ts) cells which recognize the amino terminal region of hel. in this report, the nature of the protein determinant required for ts cell induction is more precisely detailed using des-1,2,3-hel (ap-hel) prepared with an aminopeptidase purified from aeromonas proteolytica . remarkably, the removal of just these three amino acids obliterates the a ...19846202528
the use of a biotyping system to investigate an unusual clustering bacteraemias caused by aeromonas species.aeromonas spp. were isolated from blood cultures taken from four clinically bacteraemic patients over an 18 day period on four separate wards. a common source was suspected and extensive environmental sampling revealed two more ward isolates of aeromonas spp. a biotyping system was employed which distinguished the strains from each other and indicated that a common source was unlikely. this coincidental clustering occurred in the autumn, a period when isolates from water and faeces are normally ...19846205061
esterase electrophoresis: a new epidemiological screening test for aeromonas hydrophila hospital infection. 19846208255
in vitro activity of norfloxacin, a quinolinecarboxylic acid, compared with that of beta-lactams, aminoglycosides, and trimethoprim.norfloxacin is a quinolinecarboxylic acid compound. we examined the in vitro activity of this compound against gram-positive and -negative species, including anaerobic species. it inhibited 90% (mic90) of strains of escherichia coli at 0.05 microgram/ml, klebsiella sp. at 0.4 microgram/ml, salmonella and shigella spp. at 0.1 microgram/ml, citrobacter sp. at 0.4 microgram/ml, enterobacter cloacae at 0.2 microgram/ml, enterobacter aerogenes at 0.4 microgram/ml, and enterobacter agglomerans at 0.2 ...19826214995
[complex studies in the surveillance of the environmental vibrio flora in the city of gorki and gorki province].the analysis of the results of the comprehensive studies (phenotypic and genotypic properties, the precipitation test with the use of spontaneously agglutinating cultures, the determination of the antigenic structure by immunochemical analysis, etc.) carried out during the sanitary microbiological control of the environmental vibrioflora for the last 5 years are presented. only the combined use of the above-mentioned methods has allowed to ascertain the taxonomic position of atypical cultures.19826218723
[water origin of aeromonas hydrophila hospital infections]. 19836220302
[septic shock and infarction of the mesemtery in an aeromonas hydrophila septicemia]. 19836220303
in vitro activity of ciprofloxacin (bay o 9867).the in vitro activities of ciprofloxacin (bay o 9867) and seven comparative antimicrobial agents against 664 aerobic and facultatively anaerobic bacterial isolates were studied. minimal inhibitory concentrations (mics) of ciprofloxacin were less than or equal to 2 micrograms/ml for enterobacteriaceae, less than or equal to 8 micrograms/ml for nonfermentative gram-negative bacilli, less than or equal to 4 micrograms/ml for gram-positive cocci, less than or equal to 0.03 micrograms/ml for aeromona ...19836228192
in vitro activity of enoxacin, a quinolone carboxylic acid, compared with those of norfloxacin, new beta-lactams, aminoglycosides, and trimethoprim.enoxacin is a new quinolone carboxylic acid compound. its activity against 740 bacterial isolates was determined. it inhibited 90% escherichia coli, klebsiella sp., aeromonas sp., enterobacter spp., serratia spp., proteus mirabilis, and morganella morganii at less than or equal to 0.8 micrograms/ml. the majority of pseudomonas aeruginosa was inhibited by less than or equal to 3.1 micrograms/ml. haemophilus spp. and neisseria spp. were inhibited by less than 0.1 micrograms/ml. although most staph ...19836229216
[aeromonas hydrophila septicemia. epidemiologic aspects. 15 cases].fifteen cases of aeromonas hydrophila septicaemia, characterized by their frequent pulmonary lesions and the severity of their course, are reported. the delay observed between admission to hospital and first symptoms, together with the presence of anatomical lesions or physiological disturbances suggesting bacterial invasion by the intestinal route, have led the authors to postulate a nosocomial digestive contamination, probably from water, as suggested by the particular ecology of this micro-or ...19846232567
studies with enterotoxigenic microorganisms: effects of candidate antidiarrhoeals in experimental animals in vivo.chlorpromazine or aspirin alone, when given to rats parenterally, reduced intestinal fluid secretion induced by cell-free preparations of enterotoxigenic organisms including escherichia coli, aeromonas hydrophila, staphylococcus pyogenes, and salmonella typhimurium. a combination of chlorpromazine-aspirin given parenterally caused much more marked reduction of fluid secretion. indomethacin also had significant antisecretory effects against a range of bacterial enterotoxins, while loperamide was ...19846237185
induction of nonspecific immunosuppression in a primitive teleost, rainbow trout (salmo gairdneri).induction of immunological non-reactivity to goldfish red blood cells (gfrbc), sheep red blood cells (srbc) and to formalin-killed aeromonas salmonicida cells was obtained in yearling rainbow trout raised under laboratory aquarium conditions (at 10 degrees c). haemolysin and agglutinin titres were measured 1 to 4 weeks after immunization. while trout immunized with low and high doses of antigen by intramuscular (im) and intraperitoneal (ip) injections produced high antibody titres, administratio ...19846238855
in vitro activity of pefloxacin compared to that of quinolones and other antimicrobial agents.pefloxacin is a new methyl-4-piperazinyl quinolone. it had mic90 values of less than 0.01 to 0.8 micrograms/ml for the majority of escherichia coli, klebsiella, oxytoca, citrobacter, providencia, enterobacter cloacae, enterobacter aerogenes, morganella and proteus mirabilis. it inhibited ampicillin, cephalexin and nalidixic acid resistant isolates of these species. against pseudomonas the pefloxacin mic90 was 3.1 micrograms/ml. staphylococcus aureus had a mic50 of 0.4 micrograms/ml and a mic90 o ...19846241847
viruses and bacteria in pediatric diarrhea in thailand: a study of multiple antibiotic-resistant enteric pathogens.children with diarrhea admitted to a rehydration ward of a children's hospital in bangkok were investigated to determine the prevalence of enteric pathogens, the extent of children's previous antibiotic therapy, and the frequency of plasmid-mediated antibiotic resistance among infecting bacteria. rotavirus (36%), enterotoxigenic escherichia coli (18%), shigella (9%), salmonella (6%), campylobacter jejuni/coli (4%), and vibrio cholerae (2%) infections were among 105 children with diarrhea. antibi ...19816275727
elevation of adenosine 3' 5' cyclic monophosphate level by aeromonas hydrophila enterotoxin. 19816281180
[antibacterial activities of cefotiam against various pathogens isolated from clinical materials (author's transl)].bacteriological evaluation was made on cefotiam (ctm), a new broad-spectrum cephalosporin antibiotic, and the following results wer obtained. 1) ctm has shown very potent antibacterial activities against staphylococcus aureus, escherichia coli, edwardsiella tarda, citrobacter intermedius, salmonella, klebsiella, proteus mirabilis, proteus rettgeri, proteus inconstans, yersinia enterocolitica, aeromonas hydrophila, plesiomonas shigelloides and pseudomonas putrefaciens. 2) streptococcus faecalis, ...19826285018
[near-drowning in an adult: favorable course after a 20-minute submersion].cardiopulmonary resuscitation was successful in a healthy 29-year-old woman who had been submerged for 20 minutes in water at 10 degrees c. the evolution was characterized by the development of a multifactorial ards (secondary drowning) and sepsis caused by aeromonas hydrophila and acinetobacter anitratum. fibrosing alveolitis caused a restrictive syndrome with severe mechanical impairment and transitory therapy-resistant hypoxemia. it is suggested that prolonged submersion in cold water is also ...19826287569
[comparison of antimicrobial activity and stability to beta-lactamases of cefoperazone, cefotaxime, lamoxactam and ceftriaxon].ceftriaxon (ro 13-9904) is a new cephalosporin, whose structure resembles cefotaxime. this study compares the minimal inhibitory concentrations (mic-s) of ceftriaxon, cefoperazone, cefotaxime, lamoxactam and gentamicin against 622 isolates of various enterobacteriaceae, non-fermenters and aeromonas hydrophila. furthermore the minimal bactericidal concentrations (mbc-s) were determined for some organism; additionally destruction of beta-lactamantibiotics in the presence of beta-lactamases was stu ...19826289564
the effect of n-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime on non-fermentative gram-negative rods, aeromonas, plesiomonas and enterobacter agglomerans.thirty-one species (185 strains) of non-fermentative gram-negative rods (excluding pseudomonas aeruginosa) as well as 45 strains of aeromonas spp., 15 strains of plesiomonas shigelloides and 68 strains of enterobacter agglomerans were tested in microdilution procedures against n-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime. n-formimidoyl thienamycin was the most effective drug as far as the spectrum of these bacterial groups and potency is concerned; ceftazidime was ...19826293976
effect of effluent from a nitrogen fertilizer factory and a pulp mill on the distribution and abundance of aeromonas hydrophila in albemarle sound, north carolina.the density of aeromonas hydrophila, standard count bacteria, fecal coliform bacteria, and 18 physical and chemical parameters were measured simultaneously at six sites for 12 months in albemarle sound, n.c. one site was above and two sites were below the discharge plume of a kraft pulping process paper mill. the fourth site was above and the remaining two sites were below the discharge point of a nitrogen fertilizer factory. the impact of the pulp mill on water quality was acute, whereas that o ...19836297393
the in vitro activity of ceftazidime against resistant clinical isolates.the in vitro activity of ceftazidime was determined using an agar dilution technique and compared with that of cefotaxime and moxalactam against 559 clinically important isolates. ceftazidime showed poor activity against bacteroides and campylobacter, moderate activity against staphylococcus aureus and excellent activity against haemophilus, aeromonas and the enterobacteriaceae. 91% of 324 cephalothin-resistant enterobacteriaceae were inhibited at a concentration of 1 mg/l. ceftazidime was the m ...19836299967
enzymes of arginine utilization and their formation in aeromonas formicans ncib 9232.in aeromonas formicans two inducible catabolic pathways of l-arginine have been characterized. the arginine decarboxylase is induced by arginine which also induces the three enzymes of the arginine deiminase pathway but only in stress conditions such as a shift from aerobic growth conditions to very low oxygen tension. addition of glucose to medium containing arginine leads to repression of the enzymes involved in the arginine deiminase pathway while exogenous camp prevents that repression of en ...19826303241
detection of campylobacter jejuni and other potential pathogens in travellers' diarrhoea in bangladesh.to determine the distribution of pathogens associated with travellers' diarrhoea in bangladesh, we have studied 269 expatriates who presented with diarrhoea to our clinic, over a one-year period. patients were interviewed about their history of diarrhoea and a stool specimen examined for parasites and cultured for campylobacter jejuni, shigella sp., salmonella sp., vibrio and aeromonas hydrophila and plesiomonas shigelloides. c. jejuni and shigella sp. were each isolated from 45 patients (17%) a ...19836314488
purification and reconstitution in lipid bilayer membranes of an outer membrane, pore-forming protein of aeromonas salmonicida.we have purified a major outer membrane protein from aeromonas salmonicida. this 42-kilodalton protein shared several physical characteristics with enterobacterial porins in that it was noncovalently associated with the peptidoglycan, it was released from the peptidoglycan in the presence of 0.1 m nacl and sodium dodecyl sulfate, and its mobility on sodium dodecyl sulfate-polyacrylamide gels was dependent on the solubilization temperature before electrophoresis. when added to the aqueous solutio ...19836315674
survival of cultured cells after functional and structural disorganization of plasma membrane by bacterial haemolysins and phospholipases.lesions were induced in the plasma membranes of cultured human fibroblasts by membrane damaging toxins of bacterial origin (haemolysins). structural disorganization of the membrane was measured as leakage of a radiolabelled small cytoplasmic marker and functional membrane damage was measured as decreased uptake of aminoisobutyrate. cell survival was scored 24 and 48 hr later by measuring uptake of trypan blue and by light microscopical evaluation of cell morphology and proliferation. the membran ...19836318394
[severe aeromonas hydrophila infections. apropos of 2 cases].the authors describe two personal cases of severe aeromonas hydrophila infection. they review the symptomatology and treatment of such cases and insist on the unusual features in their patients.19846322344
[aeromonas hydrophila in ivory coast epidemiologic and ecologic aspects and its enteropathogenic role].a research based on the analysis of 479 coprocultures , allow the isolation of 22 strains of aeromonas hydrophila. the epidemiological aspects, the contamination processes through water, food... and the enteropathogenic role of these aeromonas are discussed. these bacteria seen responsible of digestive disturbances from simple gastric discomfort (nausea, light abdominal pain...) to the actual choleriform syndrome. the isolation of 7 strains from the environment (water from lagune , wells; lagune ...19846327100
[an aeromonas hydrophila choleriform syndrome. apropos of a case from the ivory coast].an observation of a case of choleriform syndrome of the aeromonas hydrophila subspecies anaerogenes biotype ii is reported. in a country dealing with a severe comma bacillus under endemo -sporadic from, the case report here could be wrongly diagnosed as cholera.19846327101
comparative study of the nature and biological activities of bacterial enterotoxins.it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ...19846327986
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...19846331331
resistance of aeromonas hydrophila to beta-lactam antibiotics.the activity of some beta-lactam antibiotics upon 20 strains of aeromonas hydrophila and some properties of their beta-lactamases have been studied. high degree of resistance to benzylpenicillin and lower resistance to ampicillin and cephaloridine was observed. clavulanic acid showed the highest activity. when clavulanic acid was assayed at subinhibitory concentrations in association with ampicillin. mics of the latter decreased to two- to eight-fold. all strains produced inducible beta-lactamas ...19846335147
effects of cultural conditions on protease production by aeromonas hydrophila.production of extracellular proteolytic activity by aeromonas hydrophila was influenced by temperature, ph, and aeration. conditions which produced maximal growth also resulted in maximal protease production. enzyme production appeared to be modulated by an inducer catabolite repression system whereby nh4+ and glucose repressed enzyme production and complex nitrogen and nonglucose, carbon energy sources promoted it. under nutritional stress, protease production was high, despite poor growth.19836342534
purification and characterization of a new proteolytic enzyme produced by aeromonas salmonicida.a proteolytic enzyme produced by the fish pathogen aeromonas salmonicida was isolated and purified. it showed the following characteristics: temperature optimum at 48 degrees c, ph optimum at ph 9 and a molecular weight of 87500. the enzyme was inhibited by phenylmethanesulphonylfluoride (pmsf) indicating it to be a serine protease.19836343335
in-vitro antagonism by n-formimidoyl thienamycin and cefoxitin of second and third generation cephalosporins in aeromonas hydrophila and serratia marcescens.antagonism of beta-lactam antibiotics by second and third generation cephalosporins was studied in 28 clinical strains of serratia marcescens and aeromonas hydrophila. of the antibiotics tested, both cefoxitin and n-formimidoyl thienamycin (n-f-thienamycin) were capable of antagonism in ser. marcescens (17 of 20) but in aerom. hydrophila (7 of 8), only cefoxitin induced antagonism. antagonism was observed to the greatest degree with cefotaxime and cefoperazone; intermediate with cefamandole and ...19836343337
flies as a source of enteric pathogens in a rural village in thailand.the village of ban pong in northeastern thailand was studied from january through december 1981 to determine the importance of flies as a source of enteric pathogens. the number of flies (predominantly musca domestica) increased in kitchens and animal pens in the hot dry spring, when the incidence of diarrhea was highest in the village. enterotoxigenic escherichia coli, shigella spp., non-o1 vibrio cholerae, and vibrio fluvalis were isolated from fly pools in yards (69%), animal pens (38%), bath ...19836351748
[study of various application methods for active immunization of fishes with aeromonas salmonicida, aeromonas punctata and vibrio anguillarum. 1. immunization with aeromonas salmonicida strain 314]. 19836352234
incidence of bacterial enteropathogens in foods from mexico.we examined food consumption patterns of u.s. students temporarily living in guadalajara, mexico. consumption of foods prepared in mexican homes was associated with an increased risk of acquisition of diarrhea. foods from commercial sources and private mexican homes in guadalajara were subsequently examined for contamination with coliforms, fecal coliforms, and bacterial enteropathogens. for comparison, selected restaurant foods were obtained in houston, tex. food obtained from mexican homes sho ...19836354085
[affinity chromatography of aminopeptidases].the recent data are generalized concerning a series of synthetic oligopeptides which are competitive inhibitors of aminopeptidases of animal, plant and microbic origin. a method for biospecific chromatography of these enzymes is developed, using as ligands such inhibitors as diazo derivatives of p-aminophenyl-, chloromethyl- and methylketones of l-amino acids and peptides, amino acids, aliphatic acid amides. it is established that the most effective inhibitors of aminopeptidases contain l-amino ...19836356542
a modified infant mouse assay for bacterial enterotoxins.the reproducibility of the infant mouse assay for escherichia coli st and heat-labile enterotoxins of aeromonas spp. is improved if both intestinal weight to remaining body weight ratio (iw/rbw) and the amount of diarrhoea produced are considered as criteria for classifying enterotoxigenic strains. animals with profuse diarrhoea may have iw/rbw ratios below the widely accepted critical value for a positive test. using pools of supernatants from broth cultures of three different strains of e. col ...19836362123
methods for the isolation of aeromonas hydrophila and plesiomonas shigelloides from faeces.two solid selective media, xylose deoxycholate citrate agar (xdca) and bile salts brilliant green agar (bbg) and an enrichment broth-alkaline peptone water, were evaluated for the isolation of aeromonas hydrophila and plesiomonas shigelloides. alkaline peptone water and xdca are useful for recovery of aeromonas but not plesiomonas, whereas bbg is satisfactory for both organisms.19846368683
a longitudinal study of the prevalence of bacterial enteric pathogens among adults with diarrhea in bangkok, thailand.six hundred sixty adults with diarrhea treated at bamrasnaradura hospital, bangkok, thailand were investigated to determine the prevalence, seasonality, and severity of diarrhea associated with bacterial enteric pathogens in 1980 and 1981. shigella were isolated from 27% and vibrio parahaemolyticus from 19% of the patients studied. enterotoxigenic escherichia coli (5%), salmonella (3%), non-01 vibrio cholerae (3%), campylobacter jejuni (1%), and group f vibrio (less than 1%) were isolated from a ...19836370564
use of the api 20e, oxi/ferm, and minitek systems to identify nonfermentative and oxidase-positive fermentative bacteria: seven years of experience.the api 20e (api) system, the oxi/ferm (oxf) system, and the minitek (min) system were used over a seven-year period to identify clinical isolates of nonfermentative bacteria (nfb) and oxidase-positive fermentative bacteria (opfb). a total of 742 nfb and opfb were tested with the api system, 988 with the oxf system, and 918 with the min system. the organisms represented 34 recognized species, nine alpha-numeric designates, and three species of unnamed nfb. results were compared to those obtained ...19836370567
structural and immunochemical homogeneity of aeromonas salmonicida lipopolysaccharide.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides of typical and atypical strains of the fish pathogen aeromonas salmonicida. 32p intrinsically radiolabeled lipopolysaccharide in sarcosinate-extracted outer membrane preparations, lipopolysaccharide stained by silver in proteinase k-digested outer membrane preparations and whole cell lysates, as well as purified lipopolysaccharide, displayed o-polysaccharide chains which were unusually homogeneo ...19846370955
[partial purification of the extracellular proteolytic system of aeromonas hydrophila lp50: comparative chromatographic and electrophoretic study].a complex extracellular proteolytic system was produced by aeromonas hydrophila lp50 on glucose- polypeptone medium. partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on sephacryl- s200 and chromatography on deae-sephacel. every stage was controlled by electrophoresis. this proteolytic system was constituted of three aminopeptidase and two endopeptidase components.19846372579
neuraminidase production by vibrio cholerae o1 and other diarrheagenic bacteria.vibrio cholerae o1 strains belonging to both biotypes (classical and el tor) and both serotypes (ogawa and inaba) produced neuraminidase which was released rather than cell bound. classical strains made more neuraminidase than did el tor strains. about one-third of v. cholerae non-o1 strains and one-fourth of aeromonas hydrophila strains were neuraminidase positive. strains of enterotoxigenic escherichia coli, vibrio parahaemolyticus, and shigella spp. did not produce detectable neuraminidase.19846373618
aeromonas: biology of the organism and diseases in children. 19846374630
[pathogen distribution in waste water sprinkler irrigation].the spray irrigation with pretreated wastewater was investigated on the sewage farm of the braunschweig sewage utilization association. the emission of airborne bacteria was measured by means of andersen sampler (as), reuter centrifugal sampler (rcs), and sedimentation plates (sp). there was a good correlation of results obtained by parallel measurings of as and rcs. the rcs was more effective by the factor 11.5 than the as sampling airborne microorganisms. however, the as gaves the distribution ...19846377753
bacteria isolated from lymph nodes of california slaughter swine.lymph nodes from 280 slaughter swine from 9 california ranches were examined for the presence of aerobic microflora. genera of interest which were isolated (and percentage of animals from which they were isolated) included salmonella (4.3%), beta-hemolytic streptococci (8.2%), mycobacterium avium-complex (6.4%), corynebacterium pyogenes (0.4%), and aeromonas hydrophila (5.7%). patterns of bacterial isolations from swine herds may be of assistance in predicting herd health problems.19846383150
isolation of aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates.the occurrence of aeromonas spp. in the metropolitan water supply of perth, western australia, australia, was monitored at several sampling points during a period of 1 year. water within the distribution system conformed to international standards for drinking water but contained aeromonas spp. in numbers comparable to those in raw surface water, although this water was free of escherichia coli. coliforms and e. coli were found in raw surface waters, and aeromonas spp. were found in raw water fr ...19846385848
effects of short-term cold storage on recovery of proteases from extracellular products of aeromonas hydrophila.three protease-containing fractions were recovered by gel filtration from concentrated crude extracellular products produced by aeromonas hydrophila grown in a defined medium. the recovery of a heat-stable protease was differentially prevented when the crude preparation was stored for 48 h at -20 degrees c but was unaffected by storage of the crude preparation at either 4 or -70 degrees c. once fractionated, the heat-stable protease appeared to be unaffected by subsequent storage at 4, -20, or - ...19846385854
[sensitivity of diarrhea organisms to nifuroxazide]. 19846386203
evaluation of a two-hour method for screening pathogens from stool specimens.the 2-h stool-screening test (sst) (api system s.a., montalieu-vercieu, france) was used to screen 231 organisms yielding suspicious colonies on stool differential agars for potential pathogens. all 54 salmonellae yielded correct screens. of 14 shigellas, 9 keyed as possible shigella spp.-yersinia enterocolitica-rare salmonella spp. (sys), and 5 (all shigella sonnei) keyed as possible s. sonnei-y. enterocolitica-arizona spp. (sya). three arizona strains were identified as probable salmonella spp ...19846386849
aeromonas. 19846389407
aspirin in acute gastroenteritis: a clinical and microbiological study.soluble aspirin given by mouth in divided dosage decreased intestinal fluid loss in infants and young children with acute gastroenteritis. the treated group had significantly less diarrhea, which ceased earlier and needed less intravenous therapy, than a randomly selected control group given an indistinguishable placebo. this effect of aspirin occurred with diarrhea caused by salmonella, aeromonas, escherichia coli producing heat-labile toxin, and rotavirus, but not with diarrhea associated with ...19846389815
aeromonas hydrophila and plesiomonas shigelloides as causes of intestinal infections.infections due to aeromonas hydrophila and plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. despite difficulties in characterizing the exotoxins produced by aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. some pediatric patients may have protra ...19846390638
prophylactic doxycycline for travelers' diarrhea in thailand. further supportive evidence of aeromonas hydrophila as an enteric pathogen.a randomized double-blind study to determine the efficacy of a three-week course of doxycycline (100 mg daily) in preventing travelers' diarrhea was performed in 1980 among 63 united states peace corps volunteers during their first five weeks in thailand, an area where doxycycline-resistant enterotoxigenic escherichia coli are known to be common. eight (24%) of 33 volunteers taking placebo and 3 (10%) of 30 taking doxycycline developed travelers' diarrhea for a calculated protection of 59%, but ...19846391157
[demonstration of a proteolytic activity on intracellular origin in aeromonas hydrophila lp 50].aeromonas hydrophila lp 50, isolated from packaged pasteurized milk, was grown in glucose-polypeptone medium at 30 degrees c. the proteolytic activity of a. hydrophila lp 50, optimum at the stationary phase of growth, is attributed to extracellular or membrane protease; no intracellular proteolytic activity was shown.19846391644
collaborative evaluation of the abbott avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli.the capability of the abbott avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. the avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded abbott bacterial identification cartridge plus the results of a manual oxidase test. the species included in the avantage data base are acinetobacter anitratus, acinetobacter iwoffi, aeromon ...19846392323
[evaluation of results in the identification of various aerobic gram-negative bacteria in selected bacteriological laboratories in poland]. 19846392790
the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water.a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ...19846395571
[study of aeromonas (motile) in the sewage from the city of buenos aires and water from the río de la plata].the incidence of motile aeromonas in the sewage of buenos aires city and in the water of the río de la plata was investigated. eight morphological and biochemical properties were established for the classification of bacteria in the genus aeromonas. these properties adapted from popoff and veron (19) permitted us to classify the isolated strains into two species: a. hydrophila and a. sobria. in the water of the río de la plata, of 27 isolated strains 24 were classified as a. hydrophila and 1 as ...19836400759
microbiological analysis of rock cod (sebastes spp.) stored under elevated carbon dioxide atmospheres.the numbers and types of microorganisms on fresh rock cod fillets and fillets stored in air or in a modified atmosphere (ma; 80% co(2), 20% air) at 4 degrees c were compared. samples were analyzed after 0, 7, 14, and 21 days of storage. the isolation plates were incubated aerobically, anaerobically, or under ma at 4, 20, or 35 degrees c. after 7 days of storage in air, the fillets were obviously spoiled and had a 3- to 4-log cycle increase in microbial counts. plate counts increased more slowly ...19836405691
investigation of aeromonas isolated from water; a serological study using ouchterlony and immunoelectrophoresis techniques.we have shown that immunoelectrophoresis and the ouchterlony double diffusion technique are valuable assays for the identification of aeromonas species. serological correlations have been found between the antigenic extracts originated from a collection reference strains of aeromonas hydrophila subsp. hydrophila and those originated from wild type water isolated.19836409663
a fishy tale: trout borne aeromonas hydrophila septicaemia. 19836414621
the serological properties of the cell surface proteins of vibrio cholerae.the serological properties of cell surface proteins of vibrio cholerae belonging to both the biotypes (classical and el tor) and the serotypes (ogawa and inaba) were investigated. proteins were isolated by extracting v. cholerae with edta in the presence of sodium chloride. the surface localization of these proteins was confirmed with (a) radioiodinated protein a as an immunoprobe and (b) antiserum absorption studies with whole bacteria. there were similarities among the polypeptides of cell sur ...19836415227
posttraumatic aeromonas hydrophila osteomyelitis.aeromonas hydrophila is a gram-negative organism that is the causative agent in several clinical infections. although it has been reported to cause osteomyelitis in immunocompromised patients, it has not been reported to cause this in the normal host. we describe two patients in whom acute osteomyelitis developed following trauma in freshwater lakes. cultures yielded a hydrophila, and both patients responded to a two-week course of parenteral antibiotics followed by oral tetracycline hydrochlori ...19836416197
enterotoxic activity of aeromonas hydrophila and aeromonas sobria. 19836421948
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...19846427786
vaccination of fish: a practical view.commercial vaccines giving protection against three important bacterial fish diseases (enteric redmouth, furunculosis and vibriosis) became available in the united kingdom in 1983. the degree of protection obtained and the cost effectiveness vary according to the method of presentation (injection, dipping or spraying and oral dosing). these methods and the optimum time for vaccination are described.19846431685
aeromonas spp in travellers' diarrhoea. 19846434027
aeromonas spp in diarrhoea. 19846437556
comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli.the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ...19846440476
an unusual source of hospital wound infection. 19846440623
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