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dramatic broadening of the substrate profile of the aeromonas hydrophila cpha metallo-beta-lactamase by site-directed mutagenesis.among class b beta-lactamases, the subclass b2 cpha enzyme is characterized by a unique specificity profile. cpha efficiently hydrolyzes only carbapenems. in this work, we generated site-directed mutants that possess a strongly broadened activity spectrum when compared with the wt cpha. strikingly, the n116h/n220g double mutant exhibits a substrate profile close to that observed for the broad spectrum subclass b1 enzymes. the double mutant is significantly activated by the binding of a second zi ...200515863831
evaluation of 16s rdna- and gyrb-dgge for typing members of the genus aeromonas.the dna sequence of the gyrb gene is a suitable phylogenetic marker for bacterial systematics. in this study, the diversity of aeromonas spp. present in environmental samples was assessed by a pcr combined with dgge approach. pcr primers targeting the gyrb gene of aeromonads were designed and the resulting amplicons were analyzed by dgge. the gyrb-dgge analysis was evaluated with aeromonas isolates and reference strains allowing discrimination of the majority of strains. the gyrb-dgge analysis i ...200515869956
septic arthritis due to aeromonas hydrophila: case report and review of the literature.aeromonas hydrophila is a rare human pathogen, and worldwide, soft tissue infections following water-related injuries are the most common. however, septic arthritis due to a. hydrophila remains uncommon with only seven cases previously reported in the english literature. in this report, we describe the important clinical features, microbiological findings and management of severe septic arthritis of the knee due to a. hydrophila in a healthy 13-year-old girl following an injury sustained in a pr ...200515875648
factors affecting biosurfactant production by oil degrading aeromonas spp. isolated from a tropical environment.an aeromonas spp. was isolated from tropical estuarine water. the organism grew on crude oil and produced biosurfactant that could emulsify hydrocarbons. the peak growth and biosurfactant production was on the 8th day. the organism grew on a range of hydrocarbons that include crude oil and hexadecane while no growth was recorded on some hydrocarbons that include benzene. the biosurfactant produced by the organism emulsified a range of hydrocarbons with diesel (e24=65) as the best substrate and h ...200515878609
gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities.a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ...200515880998
[influence of gaseous metabolites of soil bacteria on the multiplication of listeria monocytogenes and yersinia pseudotuberculosis].the influence of gaseous metabolites of saprophytic soil bacteria on the growth and multiplication of l. monocytogenes and y. pseudotuberculosis was studied. the study revealed that all cultures under study exhibited selectively both inhibiting and stimulating action on the multiplication of test cultures. bacteria of the genera pseudomonas and acinetobacter showed the highest inhibiting activitywith respect to the test cultures. volatile metabolites of bacteria of the genus aeromonas showed the ...200515881930
a c1q-binding 40 kda porin from aeromonas salmonicida: cloning, sequencing, role in serum susceptibility and fish immunoprotection.a 40 kda c1q-binding outer membrane protein from aeromonas salmonicida was identified by direct-binding assay with biotinylated c1q, a subcomponent of the complement classical pathway component c1. the 40 kda porin structural gene from the a450 a. salmonicida typical strain (a+:o+) was cloned in escherichia coli and sequenced. the amino acid sequence of the 40 kda a. salmonicida porin, its ability to bind c1q in an antibody independent process, and its immunological cross-reaction with the a. hy ...200515885977
the long-distance tertiary air transfer and care of tsunami victims: injury pattern and microbiological and psychological aspects.on december 26, 2004, a giant earthquake shocked southeast asia, triggering deadly flood waves (tsunami) across the indian ocean. more than 310,000 people have been reported dead and millions left destitute. shortly thereafter, european governments organized airborne home transfer of most severely injured tourists using medevac aircraft. on arrival, patients were distributed to various medical centers. one cohort of the severely injured was admitted to the cologne-merheim medical center (germany ...200515891349
microbiological quality changes in the intestine of hybrid tilapia (oreochromis niloticus x oreochromis aureus) in fresh and frozen storage condition.the goal of this study was to monitor the quantitative and qualitative bacterial flora in the intestine of hybrid tilapia in fresh fish and fish kept in frozen storage conditions for 1 year.200515892747
evaluation of an experimental aeromonas salmonicida epidemic in chinook salmon, oncorhynchus tshawytscha (walbaum).to determine the dynamics of the transmission of aeromonas salmonicida ssp. salmonicida infection, chinook salmon, oncorhynchus tshawytscha, were exposed to bacteria by cohabitation. the latent period (time between exposure and infectivity) was determined by exposing a group of chinook salmonid fingerlings to a. salmonicida by bath, then, at daily intervals, by holding five exposed (donor) fish with approximately 50 naive fish for 24 h. the latent period was 3 days post-infection and the time pe ...200515892751
sex and low-level sampling stress modify the impacts of sewage effluent on the rainbow trout (oncorhynchus mykiss) immune system.the objective of the present study was to investigate the influence of chronic exposure to municipal sewage treatment effluent at environmentally relevant concentrations on immune parameters in rainbow trout (oncorhynchus mykiss), including the assessment of potential differences in reactivity between sexually mature male and female fish. trout were exposed to 1.5 and 15% (v/v) secondary treated municipal sewage effluent for 32 weeks. fish were injected intra-peritoneally either with inactivated ...200515892994
cloning and analysis of non-specific cytotoxic cell receptor (nccrp)-1 from common carp cyprinus carpio l.nonspecific cytotoxic cell receptor protein (nccrp-1) provides an important function in target cell recognition and activation of cytotoxicity. nccrp-1 has been cloned from common carp cyprinus carpio l. from fish barbel by est analysis. the isolated gene is composed of 945 bp with a 79 bp 5' utr, 714 bp open reading frame and 152 bp 3' utr. the predicted nccrp-1 gene is composed of 237 amino acid residues and its predicted signal peptide is 19 amino acid residues in length. this gene has conser ...200515897012
aeromonas hydrophila-associated skin lesions and septicaemia in a nile crocodile (crocodylus niloticus).aeromonas hydrophila is one of the most common bacteria associated with the aquatic environment. there are, however, limited data on a. hydrophila infection in crocodilians. the aim of this report is to describe a case of skin lesions and septicaemia associated with a. hydrophila in a nile crocodile (crocodylus niloticus). a captive male crocodile in the zoological park of antalya (turkey) was found dead without showing signs of any disease. gross examination showed brown or red-spotted skin les ...200515900900
polymorphism of aeromonas spp. trna intergenic spacers.we investigated the length polymorphism of the intergenic spacers lying between trna genes of aeromonas spp. a total of 69 strains representing all known genomic species of aeromonas were used in the study. tdna-pcr patterns were examined by dice coefficient (s(d)) and unweighted pair group method of clustering (upgma). the strains were allocated into 15 groups at a similarity level of 70%. the strains belonging to seven genomic species: a. hydrophila (hg 1), a. caviae (hg 4), a. sobria (hg 7), ...200515900969
elucidation of the molecular structure of lipid a isolated from both a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharides using electrospray ionization quadrupole time-of-flight tandem mass spectrometry.the chemical structure of lipid a, isolated by mild acid hydrolysis from a rough mutant and a wild strain of aeromonas salmonicida lipopolysaccharide, was investigated using electrospray ionization quadrupole time-of-flight (qqtof) hybrid tandem mass spectrometry and showed a great degree of microheterogeneity. the chemical structure of the main constituent of this heterogeneous mixture was identified as a beta-d-(1 --> 6) linked d-glucosamine disaccharide substituted by two phosphate groups, on ...200515912470
adaptational changes in cellular fatty acid branching and unsaturation of aeromonas species as a response to growth temperature and salinity.the effects of growth temperature and salinity on the cellular fatty acids were investigated on aeromonas caviae, aeromonas hydrophila and aeromonas sobria. under optimal growth conditions, fatty acids patterns were dominated by even-numbered chains c(16:0), c(16:1cis9), c(18:1cis11), c(12:0) and c(14:0). growth temperature modifications induced, in the three aeromonas species, important changes in fatty acid (i) unsaturation, (ii) branching and (iii) chain length. an important decrease in the c ...200515925007
molecular cloning, characterization and expression analysis of qm gene from grass carp (ctenopharyngodon idellus) homologous to wilms' tumor suppressor.qm, a novel gene that was originally identified as a tumor suppressor, has been cloned from species encompassing members of higher vertebrate, plant and fungal kingdoms, but it is not well documented in fish. in present study, a gene homologous to qm was obtained from grass carp (ctenopharyngodon idellus) head kidney and spleen cdna library. the full-length grass carp qm (gcqm) cdna of 759 bp contains a short 5' utr of 22 bp, a 3' utr of 89 bp and an open reading frame of 648 nucleotides that tr ...200515936234
antibacterial effect of cu2+-exchanged montmorillonite on aeromonas hydrophila and discussion on its mechanism.montmorillonite (mmt) and its cu2+-exchanged montmorillonite (cu-mmt) were used to study the antibacterial activity on aeromonas hydrophila. the results indicated that mmt had no antibacterial activity. the minimum inhibitory concentration (mic) and bactericidal concentration (mbc) of cu-mmt on a. hydrophila are found to be 150 and 600 mg/l, respectively. the continuance of the antibacterial activity of cu-mmt was much longer than copper sulfate. in order to reveal the mechanism of the antibacte ...200515939555
attenuated virulence of an aeromonas salmonicida subsp. salmonicida type iii secretion mutant in a rainbow trout model.aeromonas salmonicida subsp. salmonicida is the causative agent of furunculosis, a severe systemic disease affecting salmonid fish. this bacterium contains a type iii protein secretion system that is responsible for the secretion and translocation of the adp-ribosylating toxin, aext, into the cytosol of fish cells. this study showed that inactivation of the type iii secretion system by marker-replacement mutagenesis of the gene ascv, which encodes an inner-membrane component of the type iii secr ...200515942017
identification of bacteria isolated from an oligotrophic lake with pesticide removal capacities.we studied the growth and capacities for pesticides removal of bacterial strains isolated from the laguna grande, an oligotrophic lake at the south of spain (archidona, málaga). strains were isolated from water samples amended with 10 and 50 microg/ml of nine pesticides: organochlorinated insecticides (aldrin and lindane), organophosphorous insecticides (dimetoate, methyl-parathion and methidation), s-triazine herbicides (simazine and atrazine), fungicide (captan) and diflubenzuron (1-(-4-chloro ...200515943106
clinical evaluation of an aerolysin-based screening test for paroxysmal nocturnal haemoglobinuria.recently, a toxin produced by aeromonas hydrophila was demonstrated to bind directly to the glycosyl-phosphatidyl-inositol (gpi) anchor. after coupling it to a fluorescent dye and applying it in fluorescence-activated cell scanning (facs), this property was exploited to detect gpi-negative cells in the diagnosis of paroxysmal nocturnal haemoglobinuria (pnh).200515952213
antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish.motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ...200515747443
family 19 chitinase from aeromonas sp. no.10s-24: role of chitin-binding domain in the enzymatic activity.family 19 chitinase from aeromonas sp. no.10s-24 (72.6 kda) is composed of two chitin-binding domains (chbds), two proline- and threonine-rich (pt-rich) linkers, and a catalytic domain. the purified enzyme was labile in a standard buffer condition and spontaneously degraded into a 46-kda fragment upon storage at 4 degrees c. the n-terminal sequence of the 46-kda fragment was found to correspond to the sequence of the c-terminal region of the second pt-rich linker, indicating that the 46-kda frag ...200515749838
evaluation of candidate probiotic strains for gilthead sea bream larvae (sparus aurata) using an in vivo approach.the aim of this study was to evaluate the effect of six bacterial strains on gilthead sea bream larvae (sparus aurata).200515752217
isolation and characterization of potentially human pathogenic, cytotoxin-producing aeromonas strains from retailed seafood in berlin, germany.the presence of potentially human pathogenic strains of aeromonas was investigated in 84 samples of seafood which were purchased from retail traders in berlin, germany in spring 2000. a total of 134 aeromonas strains were isolated on selective [gsp agar and aeromonas (ryan) agar] and unselective (standard count agar and enterohaemolysin agar) media from 27 (32.1%) of the samples and were classified as aeromonas hydrophila (67.9%), a. caviae (26.1%) and a. sobria (6.0%) by biotyping. thirteen (48 ...200515752267
aeromonas sobria, a causative agent of disease in farmed perch, perca fluviatilis l.significant numbers of perch, perca fluviatilis, raised on a pilot fish farm in switzerland presented focal skin lesions on the lateral sides and fin rot. mortality rates reached levels of up to 1% of the total fish on the farm per day. virtually pure cultures of aeromonas sobria were isolated from the liver, kidney, spleen and skin lesions of affected fish. aeromonas sobria isolated from the farmed perch had a haemolytic effect on sheep and trout erythrocytes, autoaggregated, was cytotoxic for ...200515752274
efficacy of furunculosis vaccines in turbot, scophthalmus maximus (l.): evaluation of immersion, oral and injection delivery.the commercial furunculosis vaccine aquavac furovac 5 and an autogenous vaccine, based on the challenge strain, induced immune protection in turbot, scophthalmus maximus (l.), as shown in challenge tests 120 days post-immunization by injection (relative percentage of survival, rps = 72-99%). this protective effect lasted for at least 6 months post-immunization at appreciable levels (rps = 50-52%). neither the autogenous vaccine nor the commercial vaccine was able to induce significant levels of ...200515752277
experimental infection of turbot, scophthalmus maximus (l.), by aeromonas salmonicida subsp. achromogenes and evaluation of cross protection induced by a furunculosis vaccine.turbot was shown to be sensitive to injection challenges by aeromonas salmonicida subsp. achromogenes (asa). a systemic disease was induced and the bacterium was isolated from various internal organs. histopathological changes involved haemorrhages, necrosis and degeneration in skin and muscle, haemorrhages and necrosis in kidney, degeneration in the heart muscle, and fusion of the secondary gill lamellae. a polyvalent commercial salmon vaccine, containing a. salmonicida subsp. salmonicida as on ...200515752279
adhesion and ingestion activities of fish phagocytes induced by bacterium aeromonas salmonicida can be distinguished and directly measured from highly diluted whole blood of fish.the phagocytes of fish play an important role in innate host defense against bacterial infection, and participate in various immunoregulatory processes. here, we investigated the effects of various opsonins in the ingestion and adhesion processes by examining respiratory burst (rb) activity in blood and head kidney (hk) fish phagocytes. rb activity was induced in rainbow trout phagocytes with the bacterium aeromonas salmonicida (strain mt004) in the presence of various opsonins [purified antibod ...200515752549
evaluation of dna vaccination of spotted sand bass (paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from aeromonas veronii.genes encoding two major outer membrane proteins (omps) of the bacterial pathogen aeromonas veronii, omp38 and omp48, were used to construct dna vaccines. the protective effect of such vaccines against motile aeromonad septicaemia was evaluated in spotted sand bass (paralabrax maculatofasciatus), an endemic species of the mexican northwest pacific coast and a potential resource for the aquaculture industry. weak protein expression, as determined by immunoblotting, was observed after transfection ...200515752654
biological effect of vaccination can be assessed directly from diluted whole blood of rainbow trout using homologous blood phagocytes as immunosensors.a rapid and simple method is presented for determining antibody activity following vaccination, directly from diluted fish blood. the proposed method evaluates the effects of specific antibodies on ingestion by blood phagocytes, and may be used for measuring antibody levels following vaccination. the enhancing effect of trout igm on ingestion was measured by luminol-amplified chemiluminescence (cl) emission of blood phagocytes. respiratory burst (rb) activity of blood phagocytes was induced with ...200515752656
a survey for phosphoglucose isomerase with lysyl aminopeptidase activity in vibrionaceae and non-vibrio pathogens.phosphoglucose isomerase (pgi) with a novel lysyl aminopeptidase (lysap) activity was recently purified and characterized from vibrio vulnificus. we showed that it cleaves the amino-terminal lysyl residue from des-arg(10)-kallidin to produce des-arg(9)-bradykinin, suggesting that it plays a role in virulence. a survey was conducted to determine the presence of this potential virulence-enhancing enzyme among twenty-three halotolerant human and fish pathogens from eleven species within the vibrion ...200515752701
[urinary tract infection caused by bacteria of the genus aeromonas]. 200515757594
diseases and causes of mortality among sea turtles stranded in the canary islands, spain (1998-2001).this paper lists the pathological findings and causes of mortality of 93 sea turtles (88 caretta caretta, 3 chelonia mydas, and 2 dermochelys coriacea) stranded on the coasts of the canary islands between january 1998 and december 2001. of these, 25 (26.88%) had died of spontaneous diseases including different types of pneumonia, hepatitis, meningitis, septicemic processes and neoplasm. however, 65 turtles (69.89%) had died from lesions associated with human activities such as boat-strike injuri ...200515759796
synthesis of some 1-[(n, n-disubstituted thiocar bamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives and investigation of their antibacterial and antifungal activities.fourteen new 1-[(n, n-disubstituted thiocarbamoylthio)acetyl]-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives (7a-n) were synthesised by reacting 1-(chloroacetyl)-3-(2-thienyl)-5-aryl-2-pyrazolines (5a-g) and appropriate sodium salts of n, n-disubstituted dithiocarbamoic acids (6a, b). the structures of the synthesised compounds were confirmed by elemental analyses, uv, ir, (1)h-nmr and fab(+)-ms spectral data. their antibacterial activities against proteus vulgaris (nrrl b-123), escherichia coli ...200515765490
characterization of a type iii secretion substrate specificity switch (t3s4) domain in yscp from yersinia enterocolitica.the length of the needle ending the yersinia ysc injectisome is determined by yscp, a protein acting as a molecular ruler. in addition, yscp is required for yop secretion. in the present paper, by a systematic deletion analysis, we localized accurately the region required for yop secretion between residues 405 and 500. as this c-terminal region of yscp has also been shown to control needle length it probably represents the substrate specificity switch of the machinery. by a bioinformatics analys ...200515773978
antimicrobial susceptibility of aeromonas spp., vibrio spp. and plesiomonas shigelloides isolated in the philippines and thailand. 200515784318
the antimicrobial activity of extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent.the antimicrobial activity and the mic values of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen hypogymnia tubulosa and its 3-hydroxyphysodic acid constituent have been investigated against some microorganisms. at least one of the extracts or 3-hydroxyphysodic acid showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, escherichia coli, klebsiella pneumoniae, listeria monocytogenes, proteus vulgaris, salmonella ...200515787241
nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone.bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ...200515812045
[aeromonas caviae infection mimicking inflammatory bowel disease in a child].a 10-year-old girl presented with a pancolitis with watery bloody diarrhoea. her family history revealed the presence of inflammatory bowel disease. whilst awaiting the diagnostic tests results, the stool cultures showed aeromonas caviae. the disease resolved spontaneously. after 24 months there were no further signs of illness, notably no inflammatory bowel disease. this case represents a more severe form than the mild diarrhoea that is usually described in the literature. in a retrospective se ...200515819138
production of monoclonal antibodies for detection of vibrio harveyi.monoclonal antibodies (mabs) against vibrio harveyi were produced from mice immunized with heat-killed and sds-mercaptoethanol-treated highly virulent v. harveyi 639. fifteen mabs were selected and sorted into 6 groups according to their specificity to various proteins of apparent molecular weight ranging from 8 to 49 kda. some antibodies were used for detection of v. harveyi at concentrations as low as 10(4) cfu ml(-1) using immunodot blots. most of the selected mabs did not show cross-reactivi ...200515819431
bacterial diversity in the bacterioneuston (sea surface microlayer): the bacterioneuston through the looking glass.the bacterioneuston is defined as the community of bacteria present within the neuston or sea surface microlayer. bacteria within this layer were sampled using a membrane filter technique and bacterial diversity was compared with that in the underlying pelagic coastal seawater using molecular ecological techniques. 16s rrna gene libraries of approximately 500 clones were constructed from both bacterioneuston and the pelagic water samples and representative clones from each library were sequenced ...200515819854
dietary yeast rna supplementation reduces mortality by aeromonas hydrophila in rohu (labeo rohita l.) juveniles.a feeding trial was conducted for 60 days to delineate the effect of dietary ribonucleic acid or chitin on haematological parameters, phagocyte respiratory burst and resistance to aeromonas hydrophila of labeo rohita juveniles. one hundred and twenty-six (avg. wt. 13.40 +/- 0.17 g) juveniles were randomly distributed in six treatment groups, each one in three replicates. six isonitrogenous (crude protein: 34.34-35.37%) and isocaloric (414-425 kcal 100 g(-1)) purified diets were prepared with dif ...200515820128
a millennium update on pediatric diarrheal illness in the developing world.more than one billion diarrhea episodes occur every year among children younger than 5 years of age in socioeconomically developing countries causing 2 to 2.5 million deaths. more than twenty viral, bacterial, and parasitic enteropathogens are currently associated with acute diarrhea. rotavirus and diarrheagenic escherichia coli are the most common pathogens responsible for acute diarrhea episodes in children; shigella spp., salmonella spp, campylobacter jejuni/coli, vibrio cholerae, aeromonas s ...200515825143
in-source fragmentation and analysis of polysaccharides by capillary electrophoresis/mass spectrometry.in order to develop a robust and easy-to-use technique for characterization of bacterial polysaccharides, a pseudo-hydrolysis strategy was investigated. based on in-source collision-induced dissociation, polysaccharide molecular ions were fragmented within the orifice-skimmer region of an electrospray ionization (esi) mass spectrometer. the fragment ions thus generated were then analyzed similarly to the conventional esi mass spectrometry approach. ms/ms scanning was applied to obtain product-io ...200515838845
microarray and proteomics analyses of human intestinal epithelial cells treated with the aeromonas hydrophila cytotoxic enterotoxin.we performed microarray analyses on rna from human intestinal epithelial (ht-29) cells treated with the cytotoxic enterotoxin (act) of aeromonas hydrophila to examine global cellular transcriptional responses. based on three independent experiments, act upregulated the expression of 34 genes involved in cell growth, adhesion, signaling, immune responses (including interleukin-8 [il-8] production), and apoptosis. we verified the upregulation of 14 genes by real-time reverse transcriptase-pcr and ...200515845465
spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria.in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ...200515794654
a pore-forming toxin produced by aeromonas sobria activates ca2+ dependent cl- secretion.bacteria produce many types of hemolysin that induce diarrhea by mechanisms that are not completely understood. aeromonas sobria hemolysin (ash) is a major virulence factor produced by a. sobria, a human pathogen that causes diarrhea. since epithelial cells in the intestine are the primary targets of hemolysin, we investigated the effects of ash on ion transport in human colonic epithelial (caco-2) cells. ash increased short-circuit currents (isc) in a dose-dependent manner, and it also activate ...200515797812
lack of agreement between biochemical and genetic identification of aeromonas spp.biochemical and genetic identification by rflp (restriction fragment length polymorphism) of the pcr-amplified 16s r-rna sequence were compared for a selection of 171 clinical and environmental isolates of aeromonas spp. the investigation revealed large differences between the two methods. the species phenotypic identification scheme and the genetic technique applied to the environmental strains gave divergent results for 96% of the strains tested. there was 46% discrepancy between the two metho ...200515799764
bulgecin a: a novel inhibitor of binuclear metallo-beta-lactamases.bulgecin a, a sulphonated n-acetyl-d-glucosamine unit linked to a 4-hydroxy-5-hydroxymethylproline ring by a beta-glycosidic linkage, is a novel type of inhibitor for binuclear metallo-beta-lactamases. using steady-state kinetic analysis with nitrocefin as the beta-lactam substrate, bulgecin a competitively inhibited the metallo-beta-lactamase bceii from bacillus cereus in its two-zinc form, but failed to inhibit when the enzyme was in the single-zinc form. the competitive inhibition was restore ...200515569001
modulation of the immune response to an aeromonas hydrophila aroa live vaccine in rainbow trout: effect of culture media on the humoral immune response and complement consumption.the aeromonas hydrophila aroa is an attenuated strain that has been assessed as a live vaccine in rainbow trout, oncorhynchus mykiss. in this study the effects of different culture media used to grow the strain on its survival after in vitro exposure to rainbow trout serum, and on its immunogenicity in rainbow trout were compared. four culture media were tested: luria broth (lb), luria broth with 0.25% glucose, trypticase soy broth (tsb), and brain-heart infusion broth (bhib). bacteria grown in ...200515519541
protection against experimental aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes.liposome-entrapped atypical aeromonas salmonicida antigen was prepared to investigate the potential protective efficacy for a. salmonicida infection. carp (cyprinus carpio) were immunised orally with liposome-entrapped a. salmonicida antigen. after immunisation, significantly higher antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunised control group. furthermore, immunised carp were challenged by immersion with 1 x 10(6) cfu ml(-1) of a. salmonicida for ...200515519542
bass hepcidin synthesis, solution structure, antimicrobial activities and synergism, and in vivo hepatic response to bacterial infections.bass hepcidin was purified from the gill of hybrid striped bass (morone chrysops x morone saxatilis) based on antimicrobial activity against escherichia coli. this 21-amino acid peptide has 8 cysteines engaged in 4 disulfide bonds and is very similar to human hepcidin, an antimicrobial peptide with iron regulatory properties. to gain insight into potential role(s) of bass hepcidin in innate immunity in fish, we synthesized the peptide, characterized its antimicrobial activities in vitro, determi ...200515546886
a metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase cpha and its complex with biapenem.one strategy developed by bacteria to resist the action of beta-lactam antibiotics is the expression of metallo-beta-lactamases. cpha from aeromonas hydrophila is a member of a clinically important subclass of metallo-beta-lactamases that have only one zinc ion in their active site and for which no structure is available. the crystal structures of wild-type cpha and its n220g mutant show the structural features of the active site of this enzyme, which is modeled specifically for carbapenem hydro ...200515588826
characterization of pras1-like plasmids from atypical north american psychrophilic aeromonas salmonicida.atypical psychrophilic aeromonas salmonicida isolates were obtained from farmed and wild fish in northeastern north america. these bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (tc(r)) plasmids of approximately 58 kb. the nine isolates had plasmids which could be divided into four groups based on the specific tetracycline resistance (tet) gene carried [tet(a) or tet(b)], incompatibility of the plasmid [incu or other], whether the plasmid carried the is6100 sequ ...200515621420
a pore-forming toxin produced by aeromonas sobria activates camp-dependent cl- secretory pathways to cause diarrhea.aeromonas sobria hemolysin (ash) is one of the major virulence factors produced by a. sobria, a human pathogen that causes diarrhea. we investigated the effects of ash on cl(-) transport in human colonic epithelial cells. ash increased short-circuit currents (isc) and (125)i efflux from caco-2 cells, indicating ash activate cl(-) secretion. additions of inhibitors of cyclic amp dependent cl(-) channels, glybenclamide and nppb suppressed the isc and (125)i efflux increases induced by ash. and ash ...200515621437
aeromonas hydrophila clinical and environmental ecotypes as revealed by genetic diversity and virulence genes.aeromonas hydrophila strains recovered from clinical samples and ambient sources were phenotypically and genetically identified. in addition, the distribution of putative virulence factors was assayed. to determine the genetic diversity of these strains, random amplification of polymorphic dna (rapd) and enterobacterial repetitive intergenic consensus (eric)-pcr markers were used. the discriminatory ability of the techniques, using simpson's index, was 0.96 for both methods. the most consistent ...200515621443
freshwater selenium-methylating bacterial thiopurine methyltransferases: diversity and molecular phylogeny.the diversity of bacterial thiopurine methyltransferases (btpmt) among five natural se-methylating freshwaters was investigated by polymerase chain reaction (pcr) screenings and sequencings. dna sequence analyses confirmed the cloned products' identity and revealed a broad diversity of freshwater tpmts. neighbour-joining (nj) phylogenetic analyses combining these sequences, all genbank entries closely related to these sequences and deduced tpmts obtained in this work from selected gamma-proteoba ...200515658983
antimicrobial susceptibility of uncommonly isolated non-enteric gram-negative bacilli.the frequency of occurrence and antimicrobial susceptibility patterns of 3059 non-enteric gram-negative bacilli (ngb), other than pseudomonas aeruginosa and acinetobacter spp., consecutively collected as part of the sentry antimicrobial surveillance program (1997-2003) were reviewed. during this period, a total of 221,084 bacterial isolates were collected from several clinical specimens worldwide, including 25,305 (11.5%) ngb. acinetobacter spp. and p. aeruginosa accounted for 82.7% of the ngb i ...200515664479
molecular cloning and functional analysis of two polyhydroxyalkanoate synthases from two strains of aeromonas hydrophila spp.polyhydroxyalkanoate (pha) synthase genes (phac) were cloned from two aeromonas hydrophila strains named wq and 4ak5, respectively. both strains are able to produce phbhhx copolyesters consisting of 3-hydroxybutyrate (3hb) and 3-hydroxyhexanoate (3hhx). sequence analysis showed that there was only 2 bp difference between these two pha synthase genes, corresponding to two-amino acid difference at positions of 437 and 458 of the two synthases. pha productivity and its monomer content produced by a ...200515668013
antibiotic resistance in aeromonas hydrophila and vibrio alginolyticus isolated from a wound infection: a case report. 200515674175
influence of temperature, salinity and ph on the growth of environmental aeromonas and vibrio species isolated from mai po and the inner deep bay nature reserve ramsar site of hong kong.four environmental bacterial isolates including aeromonas hydrophila mp-3, a. salmonicida mp-4, vibrio vulnificus mp-2 and v. cholerae mp-1 isolated from sediment and water of mai po nature reserve of hong kong were examined for their responses to temperature, ph and salinity under laboratory conditions in this study. v. cholerae mp-1 was found to resist vibriostatic agent o/129 at concentration of 10 microg/ml. in addition, bacterial growth under test conditions was measured and the results wer ...200515678554
aroclor 1254 exposure reduces disease resistance and innate immune responses in fasted arctic charr.to examine the immunological impacts of polychlorinated biphenyls (pcbs) in an environmentally relevant way, we orally contaminated arctic charr (salvelinus alpinus) with aroclor 1254. after contamination, fish were either fed (0 and 100 mg aroclor 1254 kg(-1) fish wt) or fasted (0, 1, 10, and 100 mg kg(-1)) to mimic cycles of feeding-fasting experienced by arctic animals. after four months, pcb concentrations in muscle were the same in fasted and fed fish; however, pcbs in kidneys of fed fish w ...200515683174
expressed sequence tags analysis of atlantic halibut (hippoglossus hippoglossus) liver, kidney and spleen tissues following vaccination against vibrio anguillarum and aeromonas salmonicida.to investigate the response of atlantic halibut to vaccination and pathogen exposure, a cdna library was constructed from liver, kidney and spleen mrna collected following vaccination against vibrio anguillarum and aeromonas salmonicida. after sequencing 1114 clones 1072 (96.23%) readable sequences were obtained of which 106 sequences are the first reported from the fish. of these, 182 clones (16.98%) contained cell/organism defence genes including immunoglobulin light chain, mhc class i and ii, ...200515683917
selective preparation of n-acetyl-d-glucosamine and n,n'-diacetylchitobiose from chitin using a crude enzyme preparation from aeromonas sp.a bacterium, aeromonas sp. gj-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. this enzyme preparation contained n-acetyl-d-glucosaminidase and n,n'-diacetylchitobiohydrolase. n-acetyl-d-glucosaminidase was inactive above 50 degrees c, but n,n'-diacetylchitobiohydrolase was stable at this temperature. utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation, n-acetyl-d-glucosamine (glcnac) ...200515685412
the flag-2 locus, an ancestral gene cluster, is potentially associated with a novel flagellar system from escherichia coli.escherichia coli k-12 possesses two adjacent, divergent, promoterless flagellar genes, fhia-mbha, that are absent from salmonella enterica. through bioinformatics analysis, we found that these genes are remnants of an ancestral 44-gene cluster and are capable of encoding a novel flagellar system, flag-2. in enteroaggregative e. coli strain 042, there is a frameshift in lfgc that is likely to have inactivated the system in this strain. tiling path pcr studies showed that the flag-2 cluster is pre ...200515687208
structural analysis of the laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars.lsl is a lectin produced by the parasitic mushroom laetiporus sulphureus, which exhibits hemolytic and hemagglutinating activities. here, we report the crystal structure of lsl refined to 2.6-a resolution determined by the single isomorphous replacement method with the anomalous scatter (siras) signal of a platinum derivative. the structure reveals that lsl is hexameric, which was also shown by analytical ultracentrifugation. the monomeric protein (35 kda) consists of two distinct modules: an n- ...200515687495
production of n-acetyl-beta-d-glucosamine from chitin by aeromonas sp. gj-18 crude enzyme.a bacterium, gj-18, having strong chitinolytic activity was isolated from coastal soil. the isolated strain was identified as aeromonas sp. by morphological and biochemical properties along with 16s rrna gene sequence. the crude chitinolytic activity of culture supernatants was maximal on the 5th day of culture. below 45 degrees c, chitin was effectively hydrolyzed to n-acetyl-beta-d-glucosamine (glcnac) by aeromonas sp. gj-18 crude enzymes, but hydrolysis decreased markedly above 50 degrees c. ...200515692805
effect of bovine lactoferrin on the mics of ampicillin/sulbactam (unasyn), amoxicillin/clavulanic acid (augmentin), ticarcillin clavulanate (timentin), and piperacillin-tazobactam (zosyn), against clinical and environmental isolates of motile aeromonas. 200515710671
high-resolution crystal structure of the restriction-modification controller protein c.ahdi from aeromonas hydrophila.restriction-modification (r-m) systems serve to protect the host bacterium from invading bacteriophage. the multi-component system includes a methyltransferase, which recognizes and methylates a specific dna sequence, and an endonuclease which recognises the same sequence and cleaves within or close to this site. the endonuclease will only cleave dna that is unmethylated at the specific site, thus host dna is protected while non-host dna is cleaved. however, following dna replication, expression ...200515713456
placental and intestinal alkaline phosphatases are receptors for aeromonas sobria hemolysin.the aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. we previously identified the putative receptor for a. sobria hemolysin as a p66 protein on intestine 407 cells (microb. pathog. 27 (1999) 215-221). here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (plap). recombinant plap expressed in 293t cells was also found to bind to hemolysin and the binding was ...200515715171
suppurative sequelae of symbiosis. 200515639303
first record of the rare species aeromonas culicicola from a drinking water supply.we describe the recovery of the rare species aeromonas culicicola, so far known only in mosquitoes in india, from a drinking water supply in spain. typing, using enterobacterial repetitive intergenic consensus-pcr, revealed that the 27 new isolates belonged to 3 very closely related strains. these strains were genetically identified by 16s rrna gene sequencing. spanish strains differed from the mosquito strains in three nucleotide positions. the ahcytoen gene was present in these water strains, ...200515640231
structural and serological characterization of the o-chain polysaccharide of aeromonas salmonicida strains a449, 80204 and 80204-1.the o-chain polysaccharide (o-ps) of aeromonas salmonicida was studied by a combination of compositional, methylation, ce-esms and one- and two-dimensional nmr analyses. it was found to be a branched polymer of trisaccharide-repeating units composed of l-rhamnose (rha), d-glucose (glc), 2-acetamido-2-deoxy-d-mannose (mannac) and o-acetyl group (oac) and having the following structure: ce-esms analysis of a. salmonicida cells from strains a449, 80204 and 80204-1 grown under different conditions c ...200515721342
effect of over-expression of phasin gene from aeromonas hydrophila on biosynthesis of copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate.the gene phapah, encoding the protein phasin that is associated with poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) granule of aeromonas hydrophila 4ak4, was cloned and characterized. recombinant strains harboring additional copies of the phasin gene (phapah) and the polyhydroxyalkanoate (pha) synthase gene (phacah) accumulated phbhhx copolyesters consisting of 21 mol% 3-hydroxyhexanoate (3hhx) as compared to 14 mol% 3hhx produced by wild type strain. the molecular weight of phbhhx produ ...200515727816
novel bromotyrosine derivatives that inhibit growth of the fish pathogenic bacterium aeromonas hydrophila, from a marine sponge hexadella sp.three new bromotyrosine derivatives, 11-n-methylmoloka'iamine (1), 11-n-cyano-11-n-methylmoloka'iamine (2), and kuchinoenamine (3), were isolated as antibacterial constituents from a marine sponge hexadella sp. their structures were elucidated on the basis of spectral and chemical methods. they exhibited moderate antibacterial activity against the fish pathogenic bacterium aeromonas hydrophila.200515730316
a vibrio vulnificus type iv pilin contributes to biofilm formation, adherence to epithelial cells, and virulence.vibrio vulnificus expresses a multitude of cell-associated and secreted factors that potentially contribute to pathogenicity, although the specific roles of most of these factors have been difficult to define. previously we have shown that a mutation in pild (originally designated vvpd), which encodes a type iv prepilin peptidase/n-methyltransferase, abolishes expression of surface pili, suggesting that they belong to the type iv class. in addition, a pild mutant exhibits reduced adherence to he ...200515731039
surveillance of bacteriological quality of natural water resources in rural areas around kasauli town, distt. solan, himachal pradesh.a study was conducted to find out the bacteriological quality of water during the monsoon season in local areas around kasauli town, and to evaluate the management of water resources in rural areas. the water samples were collected from bowries and hand pumps. as there is no municipal water supply in majority of these areas, the local residents depends mainly on these natural resources without any treatment of water. all the 14 surface water samples collected from bowries were found bacteriologi ...200517278659
[factors of virulence associated with enteropathogenicity in strains of aeromonas spp. isolated from children with diarrhea in mérida, venezuela].the feces of 397 patients with acute diarrheal disease (add) and of other 121 patients without diarrea (control group) were studied in the state of mérida, venezuela, from june 1993 to december 1994. the genus aeromonas was identified in patients with add in 11.83% and in 5.78% of the patients from the control group. on studying the virulence factors described for aeromonas (enterotoxin, cytotoxin, hemaglutinins, cellular hydrofibrosity, and hemolytic activity) in the isolated strains, it was de ...200517966577
microbiological quality of carbonated and non-carbonated mineral water stored at different temperatures.the microbiological quality of five brands of carbonated and non-carbonated mineral water sold in poland was studied. the study was carried out on the survival of heterotrophic bacteria at 22 and 37 degrees c (pour plate technique) in the samples of mineral waters stored at 4 and 22 degrees c. the one hundred ten bottles (twenty two bottles of each of the five brands) of carbonated and uncarbonated mineral waters with different levels of dissolved solids and organic content were chosen to microo ...200516457377
survival of escherichia coli and aeromonas hydrophila in non-carbonated mineral water.the study was carried out on the survival of escherichia coli and aeromonas hydrophila in samples of mineral waters. enumeration of the bacteria was performed by spread inoculation of samples (0.1 cm3) over the surface of selected media in petri plates. twenty bottles (four bottles of each of the five brands) of non-carbonated mineral waters with different levels of dissolved solids and organic content were chosen to study every strain. ten bottles were stored at 4 degrees c, the other ten were ...200516457378
mechanical transmission of pathogenic organisms: the role of cockroaches.cockroaches (diploptera punctata) are basically tropical insects and will do their best to find a home that is both warm and moist. their involvement in the transmission of tropical diseases is poorly investigated in africa.200516457381
spatial distribution of salmonella, escherichia coli o157:h7, and other bacterial populations in commercial and laboratory-scale sprouting mung bean beds.the reliability of testing spent irrigation water to assess the microbiological status of sprouting mung bean beds has been investigated. in commercial trials, the distribution of opportunistic contaminants within 32 bean sprout beds (25 kg of mung beans per bin) was assessed 48 h after germination. the prevalence of generic escherichia coli, thermotolerant coliforms, and aeromonas in sprouts (n = 288) was 5, 11, and 39%, respectively, and 57, 70, and 79% in the corresponding spent irrigation wa ...200516355820
impact of non-legionella bacteria on the uptake and intracellular replication of legionella pneumophila in acanthamoeba castellanii and naegleria lovaniensis.in aquatic environments, legionella pneumophila survives, in association with other bacteria, within biofilms by multiplying in free-living amoebae. the precise mechanisms underlying several aspects of the uptake and intracellular replication of l. pneumophila in amoebae, especially in the presence of other bacteria, remain unknown. in the present study, we examined the competitive effect of selected non-legionella bacteria (escherichia coli, aeromonas hydrophila, flavobacterium breve, and pseud ...200516341636
[identification and phylogenetic study of pathogenic bacteria causing ulcer disease of cultured turbot (scophthalmus maximus)].an outbreak of ulcer disease of the cultured turbot scophthalmus maximus l. occurred in an indoor farm in haiyang, shandong province in the sweltering august of 2004 and caused significant mortality. at the beginning, infected turbots displayed sluggish swimming and anorexia. several days later, the turbot eyes swelled, the fins and tail turned red, and the back gradually ulcerated. dissection of the moribund fish showed that the ulcerative gills paled, the liver becamed bloodshot, the kidney an ...200516342752
identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins.the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ...200516316188
etiology of childhood diarrhea in zliten, libya.to determine the etiological agents of diarrhea in children from a small semi-urban city in libya and the association of age, gender, seasonal variation, breast-feeding, source of water for drinking, or antibiotic use with the isolation of enteropathogens and whether such agents are community or hospital acquired.200516311662
high dietary vitamin c affects growth, non-specific immune responses and disease resistance in asian catfish, clarias batrachus.studies were conducted to determine the immunomodulatory effects of high dietary ascorbic acid (vitamin c) on growth, serum concentration, non-specific immune response and disease resistance of a commercially important asian catfish, clarias batrachus. four practical diets were formulated to contain 0, 500, 1000 and 2000 mg ascorbic acid (aa) equivalent/kg diet, supplied as l-ascorbyl-2-polyphosphate (lapp) and were fed for 2, 4, 6 and 8 weeks. each diet was fed to triplicate groups of catfish w ...200516311902
food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization.a modified fluorescence in situ hybridization (fish) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-proteobacteria -- aeromonas hydrophila and pseudomonas fluorescens, respectively) to grazing by three protists: the flagellates bodo saltans and goniomonas sp., and the ciliate cyclidium glaucoma. both flagellate species prefer ...200516329920
determination of the viability of aeromonas hydrophila in different types of water by flow cytometry, and comparison with classical methods.the presence of aeromonas spp. in water can represent a risk for human health. therefore, it is important to know the physiological status of these bacteria and their survival in the environment. we studied the behavior of a strain of aeromonas hydrophila in river water, spring water, brackish water, mineral water, and chlorinated drinking water, which had different physical and chemical characteristics. the bacterial content was evaluated by spectrophotometric and plate count techniques. flow c ...200516332772
cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica.cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (cis). when chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. testing of the adsorption and desorption ability of cis using various microbes such as escherichia coli, aeromonas hydrophila, pseudomonas aeruginosa, bacillus subtilis, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, ...200516332887
production of acylated homoserine lactones by aeromonas and pseudomonas strains isolated from municipal activated sludge.up to now, the production and role of n-acyl homoserine lactones (ahls) in activated sludge have been poorly understood. in this study, cross-feeding assays with the reporter strains agrobacterium tumefaciens ntl4 and chromobacterium violaceum cv026 were used to investigate ahl signal production by municipal activated sludge samples. ahl signal production was consistently detected from municipal activated sludge when different samples were incubated on nutrient media. from one municipal activate ...200516333331
development of a rapid identification method for aeromonas species by multiplex-pcr.existing biochemical methods cannot distinguish among some species of aeromonads, while genetic methods are labor intensive. in this study, primers were developed to three genes of aeromonas: lipase, elastase, and dna gyraseb. in addition, six previously described primer sets, five corresponding to species-specific signature regions of the 16s rrna gene from a. veronii, a. popoffii, a. caviae, a. jandaei, and a. schubertii, respectively, and one corresponding to a. hydrophila specific lipase (hy ...200516333335
use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum).from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ...200516336470
engulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, macrobrachium rosenbergii.haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. isolated haemocytes of m. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast debaryomyces hansenii, the bacteria aeromonas hydrophila or enterococcus faecium, or with latex beads at 25 degrees c for 2 h, followed by washing to remove free particles. at least ...200516336474
wound infections in tsunami survivors: a commentary.survivors of the december 2004 indian ocean tsunami sustained a variety of wound infections, ranging from common pathogens to rarely seen organisms. this article discusses the likely microbiology potentially seen in wound infections with exposure to freshwater, seawater, soil, faecal or other contamination, and attempts to provide an organising framework for choosing empiric antibiotics for such infections. therapy for less frequently encountered clinical entities is also discussed, including te ...200516284684
carriage by the housefly (musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in misurata, libya.using standard microbiological procedures, bacteria that are potentially pathogenic to humans were isolated from 150 houseflies collected in the libyan city of misurata (50 flies each from the central hospital, streets and abattoir). salmonella spp., yersinia enterocolitica and edwardsiella tarda were isolated from flies collected on the streets and in the abattoir but not from those collected in the hospital. shigella sonnei was detected in just one fly, which was collected in the abattoir. of ...200516297293
diversity of denitrifying bacteria isolated from daejeon sewage treatment plant.the diversity of the denitrifying bacterial populations in daejeon sewage treatment plant was examined using a culture-dependent approach. of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. according to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. this grouping was supported by an unweighted pair ...200516273028
aerobic bacterial flora of the nasal cavity in gulf of california sea lion (zalophus californianus) pups.nasal swab samples from clinically healthy california sea lions pups (zalophus californianus) from six different reproductive rookeries in the gulf of california were collected to determine the type and frequency of the representative aerobic bacterial microflora of their nasal mucosa. a total of 114 samples were examined and 100 bacterial isolates were identified and typified by microbiological and biochemical standard tests. fifty four isolates corresponded to gram positive bacteria (54%) and ...200516266850
recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ...200516269764
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