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comparison of four typing methods for aeromonas species. 19882908357
cloning and characterization of the aeromonas caviae reca gene and construction of an a. caviae reca mutant.a recombinant plasmid carrying the reca gene of aeromonas caviae was isolated from an a. caviae genomic library by complementation of an escherichia coli reca mutant. the plasmid restored resistance to both uv irradiation and to the dna-damaging agent methyl methanesulfonate in the e. coli reca mutant strain. the cloned gene also restored recombination proficiency as measured by the formation of lac+ recombinants from duplicated mutant lacz genes and by the ability to propagate a strain of phage ...19882826405
dna homology and immunological cross-reactivity between aeromonas hydrophila cytotonic toxin and cholera toxin.dna colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (ct) was used to screen 12 clinical isolates of aeromonas hydrophila. under stringent hybridizing (overnight at 40 degrees c) and washing (1 h at 50 degrees c) conditions, nine strains reacted with the 32p-labeled ct probes. concentrated (10x) cell-free supernatants or lysates from eight cultures, heated at 56 degrees c for 20 min, produced cytotonic effects in y-1 mouse adrenal cells and c ...19882830300
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...19882830310
tn5-induced protease-deficient strains of aeromonas hydrophila with reduced virulence for fish.protease-deficient strains of aeromonas hydrophila tf7 were induced by transposon tn5 mutagenesis, with escherichia coli 1830(pjb4ji) as the tn5 donor. the parent strain has the cell surface characteristics associated with virulence for fish, and as it produces a single metalloprotease, mutants could be distinguished by direct plating on brain-heart infusion skim milk agar. mutants pd-7 and pd-10 still produced metalloprotease, but at reduced levels and only after prolonged incubation. the activ ...19882843466
aerolysin from aeromonas hydrophila. 19882852762
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...19882854515
virulence factors from aeromonas species.isolates (116) of aeromonas were obtained from various sources and subjected to tests to establish their virulence factors. a high number of the isolates (69.8%) were found to be enterotoxigenic. the isolates from snails had more enterotoxigenic strains (73.3%), while those from cattle faeces had the lowest (33.3%). haemolysin production was found to be high (60.3%) amongst the isolates, and human isolates gave the highest number of haemolysin producing strains (70.6%), while the least number (3 ...19882468071
s-layer of high-virulence aeromonas hydrophila. 19882479668
serogrouping of the aeromonas hydrophila group. 19882479669
hospital-associated infection from leeches. 19883044210
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...19883045003
phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant aeromonas hydrophila from cultured channel catfish (ictalurus punctatus) and their environments.oxytetracycline-resistant (otcr) and tetracycline-resistant (tcr) aeromonas hydrophila were isolated commonly from catfish intestinal contents and the water and sediment of catfish culture ponds, but less frequently in market catfish. isolates demonstrated two resistance phenotypes, tcr otcr and tcs otcr, when plated directly on to macconkey agar containing 30 micrograms of tetracycline or oxytetracycline per ml. tcs otcr isolates expressed tcr after induction by 1 h of growth in tryptic soy bro ...19883046493
mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum.the importance of the genus aeromonas in human disease has recently become better appreciated through the use of improved methodologies for the recovery and identification of aeromonads from biologic specimens and as a result of medical studies aimed at determining the clinical significance of these bacteria when isolated from localized and invasive infections of humans. taxonomic schemes currently allow for the identification of six aeromonas species (five mesophilic and one psychrophilic), fou ...19883055195
the production of a macrophage-activating factor from rainbow trout salmo gairdneri leucocytes.rainbow trout head kidney and blood leucocytes are shown to be capable of secreting a soluble macrophage-activating factor (maf) after stimulation with concanavalin a (con a). the presence of phorbol myristate acetate (pma) as a co-stimulant increased the production of maf. both respiratory burst activity (nitroblue tetrazolium, nbt, reduction and h2o2 production) and bactericidal activity were enhanced after incubation of resident or elicited macrophages with the maf-containing supernatants for ...19883056854
differential susceptibility of salmonid fishes to furunculosis correlates with differential serum enhancement of aeromonas salmonicida extracellular protease activity.rainbow trout, atlantic salmon and brown trout, respectively, exhibit an increasing order of susceptibility to furunculosis. we investigated if this may be due to a differential sensitivity to exotoxins or ability to inhibit the exoprotease which is required by the bacterium to digest protein for growth. differences were slight in susceptibility to exotoxins and serum inhibition of exoprotease at high exotoxin:serum ratios. however, at low ratios brown trout serum markedly enhanced protease acti ...19883059141
aeromonas and plesiomonas as etiological agents. 19883059998
bacterial-induced diarrhoea.in diverse parts of the world, many bacterial strains have been recognised as the causative agents of diarrhoea. great achievements have been made in perceiving the varied mechanisms which explain their intestinal pathogenicity. these achievements include the elucidation of the 5 mechanisms of disease production by escherichia coli, the mechanisms of action of shigella, salmonella, campylobacter and yersinia, new information about antimicrobial-associated colitis caused by clostridium difficile ...19883069445
expression of met-(-1) angiogenin in escherichia coli: conversion to the authentic less than glu-1 protein.a method for obtaining authentic human angiogenin utilizing an escherichia coli recombinant expression system is described. a synthetic gene encoding angiogenin was placed into a vector for direct expression under the control of a modified e. coli trp promoter. the protein was produced by the bacteria in an insoluble form and purified to homogeneity by cation-exchange and reversed-phase hplc following reduction/solubilization and reoxidation. the protein isolated was identified as met-(-1) angio ...19883071185
incidence and biotyping of aeromonas species from the environment.a survey was carried out to establish the incidence rate of aeromonas species in various environmental samples with a view to ascertaining their reservoirs and distinguishing the biotypes. high rates of isolation were obtained from snails and water. this indicates that these samples are likely reservoirs and therefore constitute high sources of human infection. low incidence rates were realised from poultry, cattle and humans, thus suggesting that these samples may be transient hosts. two specie ...19883071728
enzymatic characterization of three aeromonas species using api peptidase, api "osidase," and api esterase test kits.an enzymatic characterization of 16 strains of aeromonas species including a. hydrophila (7), a. sobria (5), and a. caviae (4) was carried out using api peptidase (strips numbered 1, 2, 3, 4, 5, and 6); api esterase and api "osidase" test strips. a total of 89 substrates was used in the assay and included 59 arylamides (aminopeptides), 10 esters, and 20 carbohydrates. all three species were remarkably uniform in their reactivities. nineteen (32%) of the arylamide substrates used were hydrolyzed ...19883072150
aeromonas hydrophila-punctata in two sicilian reservoirs: density of the organisms in relation to physico-chemical and bacteriological parameters. 19883072527
vibrios and aeromonas.there are many similarities in the vibrionaceae that cause human illness in the united states (see table 1). vibrios are characteristically indigenous to marine, estuarine, and brackish environments. they are distributed mainly in gulf of mexico coastal water, and these organisms "bloom" when the water is warm. outbreaks of disease in humans frequently occur in summer, coinciding with multiplication of vibrios in warm water. sporadic cases and small outbreaks of cholera continue to occur in pers ...19883074121
a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...19883076719
epidemiology of aeromonas and plesiomonas diarrhoea. 19883077628
comparison of methods for the induction, propagation and recovery of l-phase variants of aeromonas spp. 19883077629
aeromonas and plesiomonas in the environment: value of differential biotyping of aeromonads. 19883077630
human extraintestinal infections caused by aeromonas species. 19883077631
drug-resistant plasmids from fish pathogens.the epidemiological surveillance of drug-resistant strains of the fish pathogenic bacteria vibrio anguillarum and pasteurella piscicida carrying transferable r plasmids in fish farms is described. the dna structure of r plasmids, and the drug-resistant determinants of r plasmids from the fish pathogens aeromonas hydrophila,aeromonas salmonicida, edwardsiella tarda, v. anguillarum, and p. piscicida are discussed.19883079184
molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from aeromonas hydrophila.the structural gene for excreted amylase from aeromonas hydrophila jmp636 has been cloned within a 2.1-kilobase smai fragment of dna. the amylase gene is transcribed from its own promoter in escherichia coli, producing a gene product of mr 49,000. the amylase gene product is secreted to the periplasm of e. coli; however, it is not excreted. nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. a potential signal peptide of ...19882449422
comparison of four hemolysin-producing organisms (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) for release of inflammatory mediators from various cells.we investigated the role of various hemolysin-producing strains (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) in induction of inflammatory mediators, e.g., histamine release from rat mast cells as well as the chemiluminescence response and the release of lipoxygenase transformation products from human polymorphonuclear neutrophils. our data show that the hemolysin-positive bacteria as well as the hemolysin-positive culture supernatants were active in i ...19882451679
gastro-enteritis in australian children: studies on the aetiology of acute diarrhoea.a prospective, year-long study of the microbiology of childhood gastro-enteritis in perth showed that (i) aeromonas-associated diarrhoea was the commonest recognized bacterial cause for acute diarrhoea, especially in summer, (ii) campylobacter infections were the next most common bacterial cause, (iii) rotavirus and parasites were not significant causes of sporadic acute childhood diarrhoea, and (iv) enteric bacterial pathogens and intestinal parasites were often isolated from aboriginal childre ...19882456732
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...19882459066
nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region.the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ...19882459581
antibiotic resistance patterns of aeromonas spp. isolated from environmental and clinical sources.microbiologists from the international centre for diarrhoeal disease research, bangladesh (icddr,b) collected water, sediment, plant, and plankton samples from a lake and a river in dhaka, bangladesh to isolate aeromonas spp. they also took clinical isolates from patients at the dhaka hospital of icddr,b. the microbiologists identified at least 154 isolates of aeromonas and analyzed them for antibiotic susceptibility using the standard agar disc diffusion method. 92.8% of the environmental is ...198812342624
[aeromonas spp. and plesiomonas shigelloides in bivalves, mud, and water from the gulf of nicoya, costa rica].bivalves, mud, and surface water were collected at three different sites of the gulf of nicoya, costa rica, in search of aeromonas spp. and plesiomonas shigelloides. for their isolation, these bacteria were enriched in alkaline peptone water and streaked on macconkey agar and on brilliant green bile inositol agar. this was followed by the biochemical tests necessary for their identification. thirty-five strains of a. hydrophila, 58 of a. caviae, 43 of a. sobria, and 7 of p. shigelloides were iso ...19899700365
bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture.aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ...198916347937
solubilization of fish proteins using immobilized microbial cells.cells of bacillus megaterium, aeromonas hydrophila, and pseudomonas marinoglutinosa were immobilized in calcium alginate. the immobilized cells secreted protease when held in fish meat suspension in water. the enzyme synthesis by the entrapped cells was supported by small amounts of soluble nutrients present in the meat. the secreted protease solubilized the fish meat, solubilization being optimum at ph range of 7.5 to 9.5 and at 50 degrees c. under these conditions immobilized b. megaterium was ...198918588026
occurrence of aeromonas hydrophila in wild birds.aeromonas hydrophila was isolated from 135 of a total of 1226 wild birds. it was the only bacterial species in eight birds, while other bacteria, mainly enterobacteriaceae, staphylococci and/or streptococci were identified in all other birds. the rate of isolation from aquatic birds (18.5%) was higher (p<0.001) than from birds of terrestrial habitats (3.4%). infection may also depend on dietary habits: 7.0% of the granivorous and herbivorous species, 8.4% of the omnivores and 12.0% of carnivorou ...198918679900
production and characterization of monoclonal antibodies to aeromonas sobria surface antigens.a panel of eight murine monoclonal antibodies (mabs) was raised against the surface antigens of aeromonas sobria isolated from a patient with diarrhoea. antibodies to the lps core and o-antigen and to protein antigens were generated. three mabs of the igm isotype against either protein or lps agglutinated 34/75 aeromonas isolates from clinical and environmental sources. similar numbers of a. hydrophila, a. sobria and a. caviae isolates were agglutinated. the mabs were screened for their ability ...19892483346
typing of aeromonas species by protein fingerprinting: comparison of radiolabelling and silver staining for visualising proteins.a method for typing aeromonas species by silver staining of total soluble proteins separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis is described. there was good agreement with the results obtained by autoradiography of whole-cell proteins for isolates examined by both methods.19892470906
typing of aeromonas strains by dna restriction endonuclease analysis and polyacrylamide gel electrophoresis of cell envelopes.the outer membrane protein (omp) composition (omp typing) of 46 fecal aeromonas strains from hybridization groups (hgs) 1 (a. hydrophila; n = 10), 4 (a. caviae; n = 16), and 8 (a. veronii; n = 20) were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a phenotypic typing method. almost every isolate of hg-1 and hg-8 had a unique omp profile, in contrast to isolates of hg-4, which were separated into five different omp types. it was possible to recognize hgs 1, 4, and 8 by ...19892473993
simple, rapid method for direct isolation of nucleic acids from aquatic environments.direct isolation of nucleic acids from the environment may be useful in several respects, including the estimation of total biomass, detection of specific organisms and genes, estimations of species diversity, and cloning applications. we have developed a method that facilitates the concentration of microorganisms from aquatic samples and the extraction of their nucleic acids. natural water samples of 350 to greater than 1,000 ml are concentrated on a single cylindrical filter membrane (type svg ...19892467621
bondage, dominance, irrigation, and aeromonas hydrophila: california dreamin'. 19892911156
aeromonas hydrophila colitis presenting as medically refractory inflammatory bowel disease. 19892912037
phenotypic characterization and dna relatedness in human fecal isolates of aeromonas spp.phenotypic characteristics were used to identify 189 aeromonas strains isolated from human feces. one hundred forty-two of these strains were placed in 11 dna hybridization groups, and the genetic and phenotypic data were compared. according to the criteria of popoff, 66% of the strains were identified as aeromonas caviae, 18% were identified as a. sobria, and 16% were identified as a. hydrophila. some biochemical characteristics differed from the criteria of popoff; 19 of 40 (48%) of tested str ...19892913025
three-dimensional structure of an open form of the surface layer from the fish pathogen aeromonas salmonicida.cell-free culture supernatants of a lipopolysaccharide (lps) o-polysaccharide-deficient, single-insertion transposon mutant of the tetragonal surface protein array (s layer)-containing fish pathogen aeromonas salmonicida were examined by electron microscopy. negative staining showed that the s layer was released as sheets of tetragonal material, indicating that although surface retention of assembled s layer requires the presence of wild-type lps oligosaccharides, initial assembly of s-layer sub ...19892914846
growth of aeromonas spp. on butzler campylobacter selective agar and evaluation of the agar for the primary isolation of aeromonas spp. from clinical specimens.the fortuitous finding that aeromonas spp. grew well on butzler campylobacter selective medium (bcsa) in a microaerobic atmosphere at 42 degrees c prompted us to evaluate the performance of bcsa for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (asba30). overall recovery rates of aeromonads from 563 stool samples from patients with acute diarrhea were higher on asba30 (70.4%) than on bcsa (56.3%); however, 21 (29.5%) grew only on bcsa. the th ...19892915030
isolation of aeromonas hydrophila from a captive caracal lynx (felis caracal).aeromonas hydrophila was isolated from the internal organs of a captive caracal lynx (felis caracal) which died of acute septicemia. grossly, patchy areas of focal necrosis were found in the lungs, liver and kidney; there was ulceration in the stomach and intestines. microscopically, lesions contained cellular debris, neutrophils, lymphocytes and gram-negative bacilli. this is the first report of isolation of aeromonas hydrophila from a captive wild animal in nigeria.19892915393
synthesis of glycoconjugates derived from various lipopolysaccharides of the vibrionaceae family.conjugation of simple ketoses (such as 3-deoxy-d-manno-2-octulosonic acid and n-acetylneuraminic acid) and of various o-specific polysaccharides (from aeromonas hydrophila and aeromonas salmonicida) to the bifunctional spacer 1,6-hexanediamine, was achieved by reductive amination. the saccharide--1-(6-amino)-hexane alkyamines obtained were converted into the corresponding isothiocyanate derivatives and coupled to the free epsilon-amino group of lysine residues of the protein carrier bovine serum ...19892920731
purification and chemical characterization of a cholera toxin-cross-reactive cytolytic enterotoxin produced by a human isolate of aeromonas hydrophila.a bacterial protein toxin possessing hemolytic, enterotoxic, and cytotoxic activities as well as cross-reactivity to cholera toxin was purified from culture filtrates of a human diarrheal isolate of aeromonas hydrophila (ssu). this cytolytic enterotoxin was purified by ammonium sulfate precipitation, hydrophobic chromatography using phenyl-sepharose, anion-exchange chromatography on deae-bio-gel a, and size-exclusion high-performance liquid chromatography. the factor was a single polypeptide wit ...19892925244
bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from aeromonas hydrophila.a cytolytic enterotoxin of molecular weight 52,000 was isolated and purified from culture supernatants of a human diarrheal isolate (ssu) of aeromonas hydrophila. the toxin reacted with cholera antitoxin when tested in an enzyme-linked immunosorbent assay and by western blot (immunoblot) analysis. the appearance of cytotoxic and hemolytic activities in culture supernatant occurred simultaneously 8 h after the initial inoculation of the culture. loss of hemolytic activity and cholera toxin cross- ...19892925245
segmental colitis associated with aeromonas hydrophila.we describe a 40-yr-old woman who presented with abdominal pain and diarrhea, and who was found to have segmental colitis involving the cecum and ascending colon. aeromonas hydrophila was isolated from the stool, and her symptoms responded to appropriate antibiotic therapy. follow-up colonoscopy confirmed complete endoscopic and histologic resolution of her colitis. this report further supports the belief that aeromonas species are pathogens in healthy humans. this organism needs to be considere ...19892929569
the suicide phenomenon in motile aeromonads.certain strains of motile aeromonas species, including all those of aeromonas caviae examined, were shown to be suicidal. when they were grown in the presence of glucose at both 30 and 37 degrees c, there was rapid die-off of the organisms after 12 h of incubation, and viable cells generally could not be recovered after 24 h. it was shown that this phenomenon was due to the production of relatively high levels of acetic acid by these strains, even during growth under highly aerobic conditions, a ...19892930167
[aeromonas hydrophila. infection in xenopus laevis].aeromonas hydrophila caused severe disease in a group of 50 xenopus leavis three weeks after being transferred from their laboratory conditions. the first clinical signs observed were marked pallor, petechiae, lethargy, anorexia, and edema. the duration of the disease was about 48 days, during which 4 animals acquired the disease and 10 died. the study of the bacteriology of the skin and skeletal muscles showed at necropsy subcutaneous edema, hemorrhage, and ascitis. aeromonas hydrophila was iso ...19892486216
aeromonas hydrophila infection complicating digital replantation and revascularization.two cases of thumb replantation and one of finger revascularization complicated by aeromonas hydrophila infection are reported. two digits were lost because of infection in soft tissue and osteomyelitis. one thumb had extensive necrosis. in all cases the infection was difficult to eradicate, probably because of ischemia. all three patients sustained their injuries while cutting meat or fish. these infection sources have not been previously reported. note is made of aeromonas hydrophila in medici ...19892490149
[yersinia and infantile gastroenteritis].fecal samples and intestinal mucosal biopsies of 16 patients in paediatric age, with protracted diarrhoea with blood and mucus, were examined for intestinal pathogens including yersinia. yersinia enterocolitica 1/5/xz and yersinia intermedia 1/18/xz were isolated from fecal samples of two patients. in another child, yersinia enterocolitica, 1/5/xz was isolated from fecal sample, while yersinia enterocolitica 1/self-agglutinable/xz from colon biopsy. the other intestinal pathogens were absent; ae ...19892490392
[aeromonas bacteremia]. 19892490659
[spontaneous peritonitis caused by aeromonas hydrophila]. 19892490661
[pleural empyema caused by aeromonas hydrophila]. 19892490662
[endocarditis caused by aeromonas hydrophila]. 19892490663
[acute salpingitis caused by aeromonas hydrophila]. 19892490664
[aeromonas]. 19892490668
[intestinal infections caused by aeromonas].we have identified 38 instances of intestinal infection by aeromonas and 4 by plesiomonas in 16,184 coprocultures carried out during a 4 year period in the hcu of zaragoza. the low prevalence can be related with the fact that specific media for the recovery of these organisms were not used. the species aeromonas sobria (47.36%) and a. caviae (39.47%) were more common than a. hydrophila (13.15%). aeromonas were isolated from patients with and without intestinal disease. the occurrence of gastroin ...19892490673
[application of the heiberg biotypes in the diagnosis of species in the mesophilic aeromonas group]. 19892490869
[bacterial etiologies of acute infantile diarrhea in dakar].systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica.19892491376
in-vitro activity of lomefloxacin in comparison with pefloxacin and ofloxacin.the in-vitro activity of lomefloxacin (sc 47111, ny-198) was investigated by the determination of mics in agar and in broth, of mbcs in broth, of killing curves and of the duration of the post-antibiotic effect. mics measured in broth and in agar were almost identical. lomefloxacin was two- to eight-fold less active against gram-positive bacteria than ofloxacin. its activity against staphylococcus aureus was independent of resistance to penicillin and oxacillin. the activity of lomefloxacin, ofl ...19892496071
antimicrobial susceptibility of sixty human fecal isolates of aeromonas species.the mics of 21 antimicrobial agents were determined for 60 strains of aeromonas spp. isolated from human feces. all isolates tested were susceptible to aztreonam, tetracycline, imipenem, moxalactam, pipemidic acid, gentamicin, trimethoprim-sulfamethoxazole, pefloxacin and ciprofloxacin. resistance to erythromycin and streptomycin was observed in all 60 strains. aeromonas caviae was less susceptible to cefamandole, cefotaxime, norfloxacin, chloramphenicol, tetracycline, sulfamethoxazole and trime ...19892496995
isolation and characterization of monoclonal antibodies against alkaline phosphatase of pseudomonas aeruginosa.monoclonal antibodies against the alkaline phosphatase of pseudomonas aeruginosa were produced from spleen cells of balb/c mice primed with purified alkaline phosphatase of p. aeruginosa atcc 10145 and sp20/ag-14 myeloma cells. the eight stable clones established produced antibodies that reacted by enzyme-linked immunosorbent and indirect immunofluorescence assays with all bacterial strains of p. aeruginosa, including the 17 serotypes and two nontypable strains. three of the clones cross-reacted ...19892501343
aeromonas: the forgotten pathogen. 19892504945
lomefloxacin (sc 47111 or ny-198), a new difluorinated quinolone: comparison of the in vitro activity with other broad spectrum antimicrobials against enterobacteriaceae, acinetobacter spp, aeromonas spp, and pseudomonas aeruginosa.using a broth microdilution method, we compared the in vitro activity of lomefloxacin to other broad spectrum antimicrobials against clinical strains of enterobacteriaceae, acinetobacter spp, aeromonas spp, and p. aeruginosa. against the enterobacteriaceae and a. hydrophila, lomefloxacin showed excellent activity with mic50 values ranging from 0.12-0.5 micrograms/ml and mic90 values ranging from 0.12-1.0 micrograms/ml. by comparison, lomefloxacin had superior activity to nalidixic acid and diflo ...19892507219
complexing of copper in drinking water samples to enhance recovery of aeromonas and other bacteria.the presence of copper in drinking water samples at concentrations as low as 10 micrograms/l resulted in a rapid die-off of aeromonads. coliform bacteria, heterotrophic plate count bacteria and faecal streptococci were also sensitive to copper but to a lesser degree than aeromonads. the effect was particularly noticeable in soft water (less than 3 meq/l ca + mg) and at ph-values below 8.0. the toxic effect of copper concentrations up to 500 micrograms/l could be neutralized for a period of up to ...19892512276
influence of modified-atmosphere storage on the growth of uninjured and heat-injured aeromonas hydrophila.the growth of uninjured and heat-injured aeromonas hydrophila incubated at 5 degrees c (22 days) and 30 degrees c (31 h) under air, n2, and co2 was investigated. at 30 degrees c, the growth patterns of cells on brain heart infusion agar incubated under air and n2 were similar, although slight differences in the lengths of the lag phases and the final populations were detected. the lag phases of cells incubated under air and n2 were substantially longer at 5 degrees c than at 30 degrees c. the po ...19892516434
evolutionary relationships among bacterial carbamoyltransferases.an immunological approach was used for the study of ornithine carbamoyltransferase (otcase) evolution in bacteria. antisera were prepared against the anabolic and catabolic otcases of pseudomonas aeruginosa and aeromonas formicans as well as against otcase and putrescine carbamoyltransferases from streptococcus faecalis; these antisera were then tested against the unpurified otcases, either anabolic or catabolic, of 34 bacterial strains. extensive cross-reactions were observed between the antise ...19892516870
purification and characterization of aeromonas hydrophila beta-hemolysin.aeromonas hydrophila hemolysin was excreted in our culture conditions during the stationary growth phase. the toxin was purified to homogeneity by a three-step method: ultrafiltration, acid precipitation in the presence of rna and anion exchange chromatography with fplc apparatus. beta-hemolysin is a protein not associated with lipids, carbohydrates or nucleic acids whose subunit mol. wt is 51,000. the mol. wt determined by polyacrylamide gel electrophoresis suggests that the molecule is in a tr ...19892516922
[bacterial isolation in infants hospitalized for acute diarrhea].bacteria were investigated in stools of 156 children under two years of age admitted to the pediatric wards of a general hospital at the western metropolitan area of santiago, chile, because of acute diarrhea with only one stool sample. a known pathogenic agent was isolated from 115 cases (73.7%), this being a bacteria in 87/115 (75.6%). most prevalent microorganisms were enteropathogenic escherichia coli (epec). (65.5%), specially serogroups 0111, 0119 and 055, enterotoxigenic escherichia coli ...19892520838
amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in aeromonas hydrophila.aeromonas hydrophila 495a2 excreted two forms of amonabactin, a new phenolate siderophore composed of 2,3-dihydroxybenzoic acid, lysine, glycine, and either tryptophan (amonabactin t) or phenylalanine (amonabactin p). supplementing cultures with l-tryptophan (0.3 mm) caused exclusive synthesis of amonabactin t, whereas supplements of l-phenylalanine (0.3 to 30 mm) gave predominant production of amonabactin p. the two forms of amonabactin were separately purified by a combination of production an ...19892522922
cutaneous manifestations of bacterial sepsis.in short, bacterial sepsis is associated with a number of peripheral manifestations involving the skin and soft tissues. the pathogenesis of the lesions observed is not fully understood and is almost certainly multifactorial. in ecthyma gangrenosum, the presence of large numbers of gram-negative bacilli in the walls of small blood vessels without a substantial inflammatory response suggests that either the bacteria themselves or bacterial products are responsible for tissue damage. endotoxin pro ...19892523895
genetics and molecular biology of siderophore-mediated iron transport in bacteria.the possession of specialized iron transport systems may be crucial for bacteria to override the iron limitation imposed by the host or the environment. one of the most commonly found strategies evolved by microorganisms is the production of siderophores, low-molecular-weight iron chelators that have very high constants of association for their complexes with iron. thus, siderophores act as extracellular solubilizing agents for iron from minerals or organic compounds, such as transferrin and lac ...19892531838
physiological control of amonabactin biosynthesis in aeromonas hydrophila.amonabactin is a siderophore from aeromonas hydrophila which is produced in two biologically active forms composed of the phenolate 2,3-dihydroxybenzoic acid (dhb), lysine, glycine, and either trytophan (amonabactin t) or phenylalanine (amonabactin p). amonabactin biosynthetic mutants (generated by chemical mutagenesis) that either produced no amonabactin or overproduced the siderophore were isolated and identified on chrome azurol s siderophore detection agar. amonabactin-negative mutants were ...19892535179
[infectious gastroenteritis in the immunocompetent child. significance of cryptosporidium spp. and aeromonas ssp].over a one-year period we have examined 390 stool specimens from 210 children hospitalized for infectious gastroenteritis or in whom infectious gastroenteritis occurred during hospitalization. the most prevalent pathogens were rotaviruses (22.4%), followed by enteric salmonella (8.1%), campylobacter jejuni/coli (2.4%), cryptosporidium spp. (2.4%) and aeromonas spp. (1.9%). both cryptosporidium spp. and aeromonas spp. are important pathogens of infectious gastroenteritis in immunocompetent childr ...19892540525
[the occurrence of escherichia coli, aeromonas hydrophila, plesiomonas shigelloides and clostridium perfringens in the intestinal flora of gray herons (ardea cinerea)].the flora of the large intestine of 92 grey herons was examined for the frequency of aerobic and microaerobic growing bacteria. clostridium perfringens, aeromonas hydrophila, plesiomonas shigelloides and e. coli were isolated from 55%, 48%, 14% and 35% of the birds, respectively. it could be demonstrated that the findings of these bacteria in the intestinal flora are depending on the age of the birds. the percentage of carriers of clostridium perfringens, aeromonas hydrophila and plesiomonas shi ...19892548355
protection of rainbow trout against vibriosis and furunculosis by the use of attenuated strains of vibrio anguillarum.the fish pathogen vibrio anguillarum causes a lethal infection in rainbow trout (salmo gairdneri). three different avirulent mutants, constructed by transposon insertion mutagenesis (van20 and van70) or as antibiotic-resistant mutants (van1000), were isolated by screening 200 individual isolated mutants for avirulence. when used as live vaccines, all three avirulent mutants were able to induce protective immunity against the homologous as well as a heterologous strain of v. anguillarum. when van ...19892548446
comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate.the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ...19892552904
relation between aeromonas and faecal coliforms in fresh waters.a possible correlation between the presence of mesophilic aeromonads and the number of faecal coliforms present in three fresh-water habitats subject to differing levels of faecal pollution was investigated. concentration of aeromonas spp. between 10(2)-10(9) cfu/100 ml and faecal coliforms of between 9-10(7) cfu/100 ml were found in the waters. in water free from faecal pollution there was no correlation but in polluted waters there was a significant relationship between the numbers of aeromona ...19892553653
enterotoxin induced diarrhoea--an update.the pathogenic personality or the criteria required to be a successful pathogen, of enteric bacteria includes, among others, the ability to produce potent proteins which by different intracellular mechanisms elicit what we overtly see as diarrhoea. enteropathogens belonging to several genera like vibrio, escherichia, shigella, salmonella, campylobacter, aeromonas and yersinia include species capable of elaborating strikingly similar exotoxins which seem to share common mechanisms of action invol ...19892554547
recovery of viruses and bacteria in waters off bondi beach: a pilot study.a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ...19892556634
aeromonas species in aquaria: a reservoir of gastrointestinal infections?to assess the role of aquaria in the epidemiology of aeromonas associated gastroenteritis, the prevalence and antibiotic susceptibility of aeromonads in ornamental aquaria were determined and compared to that of isolates obtained from patients with gastroenteritis. thirty-eight (76%) of 50 aquaria, including those located in our hospital, yielded 61 isolates: 24 (39.3%) aeromonas sorbria, 18 (29.0%) a. hydrophila, 15 (24.6%) a. caviae, and four (6.5%) which could not be speciated. in comparison, ...19892567310
characterization of a pilus produced by aeromonas hydrophila.a pilus produced by aeromonas hydrophila was purified and partially characterized. the pilin monomers had an apparent molecular weight of 17,000. agglutination studies indicated serological cross-reactivity in the pili of a. hydrophila strains. presence of pili did not correlate with hydrophobicity or haemagglutinating ability of the bacteria.19892569431
motile aeromonas infection of striped (grey) mullet mugil cephalus.infection of striped mullet (mugil cephalus) with aeromonas hydrophila results in an acute septicemic disease. the disease can be experimentally induced by intramuscular injection, skin or gill scarification or by the oral route using pellets purposely seeded with bacteria. the organism was isolated from the blood 1-2 days after infection and from all organs 24 hr or longer after infection. the disease is characterized by early inflammatory and proliferative changes and later necrotic changes. e ...19892575883
conjugation system of incc plasmid ra1, and the interaction of ra1 pili with specific rna phage c-1.ra1 was the only incc plasmid that was slightly temperature-sensitive for replication and transfer. at 30 degrees c, ra1 determined constitutive synthesis of conjugative pili and yet was transfer-repressed. attachment of shaft-adsorbing rna phage c-1 virions prevented the probable retraction of pili under heat stimulus (55 degrees c). electron microscopy showed single adsorbed virions at pilus bases where they were thought to have stopped retraction.19892576151
bombesin: an activator of specific aeromonas antibody secretion in rat intestine.the effect of bombesin (bbs) in modulating the secretion of specific aeromonas antibodies in rat intestine was determined. rats were immunized with the culture supernatant of aeromonas hydrophila, isolate ssu. this culture supernatant contained a number of toxins that may be considered virulence factors. after 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was perfused with phosphate-buffered saline. the effluents were collected for measurement of iga and igg by t ...19892582984
chronic colitis after aeromonas infection. 19892583575
hygienic status of food handlers.the study demonstrated bacterial species on hands and nails of food-handlers before and after hand-washing. those were staphylococcus spp., streptococcus spp., micrococcus spp., bacillus spp., diphtheroid, aeromonas hydrophila, klebsiella pneumoniae, acinetobacter, enterobacter cloacae, escherichia coli, pseudomonas spp., proteus mirabilis, serratia spp., citrobacter freundii. before hand washing, each food-handler harboured one to eight bacterial species. after hand-washing (eight with water fr ...19892584903
aeromonas hydrophila strains with moderate virulence.a group of aeromonas hydrophila strains was obtained from very different origins which showed very similar characteristics (lipopolysaccharide, outer-membrane protein profile, toxin production, bacteriophage sensitivity and moderate virulence), besides the heterogenicity observed among the a. hydrophila strains. this group of a. hydrophila strains with moderate virulence can be easily recognized by their bacteriophage sensitivity pattern.19892593869
the germ-spectrum problem and antibiotic-resistance in bida and minna hospitals--nigeria.in the present paper the occurrence of different species of pseudomonas, of acinetobacter calcoaceticum var. anitratum and aeromonas hydrophila in hospitals is described. the possible human pathogenic significance is discussed.19892599385
[infectious diarrhea in the adult].infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ...19892602888
mixed infections in childhood diarrhoea: results of a community study in kiambu district, kenya.one thousand four hundred and twenty diarrhoea specimens from 846 children aged 0 to 60 months were collected and analysed for bacteria, parasites and rotavirus over a 16 month period, from june 1985 to september 1986 inclusive. the study was conducted in 4 villages situated in kiambu district, kenya. all the specimens were analysed for rotavirus and parasites, including cryptosporidium. the majority of the specimens were analysed for enteropathogenic escherichia coli (epec), shigella, salmonell ...19892606013
some observations on the faecal carriage of mesophilic aeromonas species in cows and pigs.replicate faecal samples from healthy individual pigs and cows were examined for the presence of aeromonas sp. over a 12-month period. aeromonads were found to be minor components of the faecal flora, only 8.8% of 520 samples from pigs and 4.6% of 481 samples from cows proving positive. isolation rates in both groups of animals were seasonal. a hydrophila (62% of the isolates) was the predominant species in cows, followed by a. caviae (32%) and a. sobria (15%). this pattern was also recorded in ...19892606161
serogroups of aeromonas species from clinical and environmental sources in calcutta, india.using a recently developed somatic o-antigen serogrouping scheme, 118 isolates of aeromonas spp. were serogrouped, 68 of which were isolated from diarrhoea patients admitted to the infectious diseases hospital and b.c. roy children's hospital, calcutta, from march 1987 to february 1988 and 50 randomly selected from an environmental study conducted during the same period. the isolates were typed into 23 different serogroups which represent 35.9% of the total 64 o-serogroups in the typing scheme. ...19892607101
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