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homology of saccharomyces cerevisiae adh4 to an iron-activated alcohol dehydrogenase from zymomonas mobilis.insertion of the transposable element ty at the adh4 locus results in increased levels of a new alcohol dehydrogenase (adh) activity in saccharomyces cerevisiae. the dna sequence of this locus has been determined. it contains a long open reading frame which is not homologous to the other adh isozymes that have been characterized in s. cerevisiae nor does it show obvious homology to drosophila adh. the hypothetical adh does, however, show strong homology to the sequence of an iron-activated adh f ...19872823079
effects of polymyxin b nonapeptide on aeromonas salmonicida.in contrast to polymyxin b-susceptible gram-negative bacteria of human origin, the fish pathogen aeromonas salmonicida was resistant to sensitization by polymyxin b nonapeptide (pmbn) to hydrophobic antibiotics. similarly, sensitization of a. salmonicida strains by pmbn to the bactericidal action of brook trout (salvelinus fontinalis) serum complement was less pronounced than the similar effect of pmbn against other gram-negative bacteria in certain mammalian sera. the surface array protein (a l ...19872823698
molecular analysis of bacterial cytolysins.results of molecular and pathogenic studies of three different bacterial hemolysins (cytolysins) are presented. these exoproteins derive from the two gram-negative bacteria escherichia coli and aeromonas hydrophila and from the gram-positive pathogen listeria monocytogenes. the hemolysin of e. coli is determined by an 8-kilobase (kb) region that includes four clustered genes (hlyc, hlya, hlyb, and hlyd). this hemolysin determinant is part either of large transmissible plasmids or of the chromoso ...19872825323
post-transplant peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.two patients previously managed by continuous ambulatory peritoneal dialysis for end stage renal failure received cadaveric renal transplants. the peritoneal catheter was capped off and left in situ postoperatively. both patients developed bacterial peritonitis shortly after transplantation. it was felt that the infections were associated with the presence of the indwelling peritoneal catheter as there was no clinical evidence of peritonitis at the time of transplantation.19872886532
[effect of silver preparations on the microflora regenerated from a condensate of atmospheric water vapor in a hermetically-sealed chamber].the effect of different silver compounds (silver sulphate, silver nitrate, electrolytic ionic silver) on microorganisms in water reclaimed from the atmospheric condensate in an enclosed environment was investigated. the following microorganisms were examined: alcaligenes faecalis, citrobacter freundii. aeromonas hydrophila, staphylococcus epidermidis, etc. the ionic silver concentrations in solution varied from 0.1 to 10 mg/l. the efficiency of the silver compounds was found to depend on the mic ...19872886695
the recent evolutionary origin of the phenylalanine-sensitive isozyme of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase in the enteric lineage of bacteria.evolutionary events that generated the three regulatory isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthase present in contemporary strains of escherichia coli have been proposed recently [ahmad et al. (1986) j bacteriol 165:146-154]. the phylogenetic subdivision of gram-negative prokaryotes studied (superfamily b) includes enteric bacteria, an oceanospirillum cluster, pseudomonad group i (e.g., pseudomonas aeruginosa), pseudomonad group v (e.g., xanthomonas), and the acineto ...19872888901
seasonal prevalence of nosocomial aeromonas hydrophila infection related to aeromonas in hospital water.a seasonal variation in nosocomial aeromonas hydrophila infection was correlated with the number of aeromonas in the hospital water supply. the high summer prevalence of a. hydrophila infection coincided with periods when water counts from storage tanks were highest. the waterborne origin of these infections highlights the importance of maintaining clean water supplies, especially where storage tanks are used. monitoring a. hydrophila in hospital water, particularly during the summer months, may ...19872889768
purification of cytotoxic enterotoxin of aeromonas sobria by use of monoclonal antibodies.cytotoxic enterotoxin of aeromonas sobria was purified by affinity chromatography with monoclonal antibodies. the purified enterotoxin gave a single protein band in polyacrylamide gradient gel electrophoresis and its mol. wt estimated by this technique was 63,000; it had a pi of 6.2. the purified enterotoxin caused fluid accumulation in rat ileal loops and in infant mice, was cytotoxic to cultured cells, was haemolytic to human erythrocytes, and was lethal to mice after intravenous injection. th ...19873104592
medical leeches as sources of wound infection. 19873107664
in vitro activity of ro 23-6240 (am-833): a new fluoroquinolone.the in vitro activities of ro 23-6240 (am-833) and four comparative fluoroquinolones were studied. minimal inhibitory concentrations (mics) of ro 23-6240, which inhibited at least 90% of strains, were less than or equal to 0.03-1 microgram/ml for staphylococcus aureus, staphylococcus epidermidis, enterobacteriaceae, acinetobacter anitratus aeromonas hydrophila, branhamella catarrhalis, and haemophilus influenzae, 2-8 micrograms/ml for staphylococcus saprophyticus, streptococci, diphtheroids, and ...19873107872
enumeration of pathogenic bacteria from sewage sludge in kuwait.domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ...19873121985
construction of a transposon containing a gene for polygalacturonate trans-eliminase from klebsiella oxytoca.a dna fragment containing a klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon tn5. this new transposon, designated tn5-pga+, had a transposition frequency of 1 x 10(-6). the broad host range plasmid pr751::tn5-pga+ was conjugally transferred to a variety of genetic backgrounds. the ability to degrade polygalacturonate was expressed in aeromonas hydrophila, alcaligenes eutrophus, azotomonas insolita, escherichia coli, pseudomonas put ...19873034186
catabolism of arginine, citrulline and ornithine by pseudomonas and related bacteria.the distribution of the arginine succinyltransferase pathway was examined in representative strains of pseudomonas and related bacteria able to use arginine as the sole carbon and nitrogen source for growth. the arginine succinyltransferase pathway was induced in arginine-grown cells. the accumulation of succinylornithine following in vivo inhibition of succinylornithine transaminase activity by aminooxyacetic acid showed that this pathway is responsible for the dissimilation of the carbon skele ...19873129535
virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden. 19873687646
comparison of selective media for primary isolation of aeromonas species from human and animal feces.five selective media were evaluated for their effectiveness in the primary isolation of aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. sheep blood agar with 30 mg of ampicillin per liter (asba30) yielded a significantly higher percentage of positive specimens as compared with the four other media. the effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated aeromonas spp. ...19873693536
clinical significance of aeromonas species isolated from patients with diarrhea.a total of 248 strains of aeromonas spp. were isolated from 3,334 human fecal specimens submitted to a state public health laboratory over a 2-year period to be cultured for enteric pathogens. cary-blair transport medium, blood ampicillin agar, and alkaline peptone water enrichment provided optimal recovery of aeromonas spp. a questionnaire requesting clinical and epidemiological information was sent to physicians, who submitted stool samples for testing, with each laboratory report for 107 cons ...19873693537
evaluation of quantum ii microbiology system for identification of gram-negative bacteria of veterinary origin.the ability of a rapid, semiautomated bacterial identification system, the quantum ii microbiology system (abbott laboratories, irving, tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. a total of 378 isolates were tested, including 298 organisms in the family enterobacteriaceae and strains representing acinetobacter sp., aeromonas sp., flavobacterium sp., pasteurella multocida, plesiomonas sp., and pseudomonas spp. of these isolates, 333 (88.1%) were co ...19873693539
isolation of aeromonas sp from faecal specimens. 19873693573
aeromonas hydrophila wound infection associated with myonecrosis and gas gangrene. 19873693881
aeromonas in the gut. 19873693922
typing of aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins.a method for typing species of aeromonas by 35s-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (sds-page) of total soluble proteins is described. radiolabelled-protein profiles from different strains were compared by use of the dice coefficient. typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. the results also yielded information on subgroups of phenotypic species.19873656393
l-methionine gamma-lyase from pseudomonas putida and aeromonas. 19873657560
seafood shucking as an etiology for aeromonas hydrophila infection.aeromonas hydrophila has been increasingly documented as a hardy organism responsible for severe infection in the compromised host. this case report illustrates how a hydrophila may survive prolonged freezing and how seafood shucking may cause sepsis. this report serves as a caution to the immunocompromised seafood lover.19873662710
detection, quantitation and stability of the beta haemolysin of aeromonas spp.amongst 58 isolates of motile aeromonads evaluated for the ability to produce beta haemolysin, haemolytic activity was significantly associated with strains belonging to the aeromonas hydrophila and a. sobria groups. of erythrocytes from nine animal species tested, mouse red blood cells provided the best indicator system for detection of beta-haemolysin activity. furthermore, differences in the stability of the beta haemolysins of selected a. sobria and a. hydrophila isolates at different temper ...19873669057
the bacteriological quality of bottled natural mineral waters.fifty-eight bottles of natural mineral water, taken from the point of sale, were bacteriologically examined. no coliforms or aeromonas sp. were isolated from any sample. high total bacterial counts were found particularly in the still waters. most of the organisms isolated in the total counts were gram-negative rods, but gram-positive organisms were also isolated. gram-positive cocci were further identified, some of which were known human commensals suggesting contamination of the waters prior t ...19873678404
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...19873679544
acute diarrhoea in adults: a prospective study.a prospective study of 74 adults with acute diarrhoea was carried out in sydney in 1984-1985 to determine the infective agents that were involved and their relationship to clinical, epidemiological and laboratory features. thirty-four potential pathogens were identified in 32 (43.2%) patients. these included, in order of frequency: campylobacter spp., rotavirus, clostridium difficile, salmonella enteritidis, aeromonas hydrophila, vibrio parahaemolyticus, adenovirus, a small round virus and giard ...19873683260
adhesion of aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells.the adhesion of aeromonas salmonicida, the pathogenic bacterium of fish furunculosis in salmon and trout, to the surface of host tissue cells was investigated with two fish tissue culture cell lines (rtg-2 cells from rainbow trout, salmo gairdneri, and chse-214 cells from chinook salmon, oncorhynchus tshawytscha) and four a. salmonicida strains. bacterial cells of pathogenic strains were highly adhesive to rtg-2 and chse-214 cells and were negatively charged in the net electrostatic charges, as ...19873818093
interaction of thromboxane a2 and tissue pathology during graded bacteremia.the purpose of this study was to determine whether or not thromboxane a2 (txa2) was necessary or sufficient for the development of end-organ pathology during graded bacteremia. pulmonary artery catheters were placed in 21 adult male pigs under pentobarbital anesthesia and breathing room air. after a control period, animals were studied in four groups: group 1, anesthesia only; group 2, infusion of 1 x 10(9) ml aeromonas hydrophila which was gradually increased from 0.2 ml/kg/hr to 4.0 ml/kg/hr o ...19873820352
aeromonas-associated diarrhea in the netherlands. 19873826975
importance of aeromonas sobria in aeromonas bacteremia. 19873805781
a modification of bile salts brilliant green agar for isolation of motile aeromonas from foods and environmental specimens.aeromonas has recently been recognized as an important enteropathogen. in faecal specimens the organism is readily identified and differentiated from coliforms by the positive oxidase reaction. however, few media have proved useful for foods and environmental specimens. bile salts brilliant green starch agar (bbgs) was studied for this purpose by testing artificially contaminated foods as well as natural potential sources. the results indicate the suitability of the medium for the isolation of a ...19873595749
activities of aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes.the activity of hemolysin produced by aeromonas hydrophila ca-11, isolated from an environmental source, was more sensitive to temperature than that of hemolysin produced by strain ah-1, isolated from a diarrheal case. ca-11 hemolysin failed to elicit hemolysis below 10 degrees c. immunoblotting analyses showed that both hemolysins formed into oligomers in rabbit erythrocyte membrane even when no hemolysis occurred. suggesting that the binding and the subsequent oligomerization are temperature i ...19873596803
ampicillin-dextrin agar medium for the enumeration of aeromonas species in water by membrane filtration.published selective media were evaluated for the isolation of aeromonas spp. from environmental samples by membrane filtration. satisfactory recoveries were obtained only with ma agar (rippey & cabelli) and dextrin-fuchsin-sulphite agar (schubert), but neither was sufficiently selective. the positive aspects of these two media were combined in a new medium, ampicillin-dextrin agar. recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and inc ...19873597206
hydroxamate-induced spectral perturbations of cobalt aeromonas aminopeptidase.the absorption spectrum of cobalt(ii)-substituted aeromonas aminopeptidase is markedly perturbed by the presence of equimolar concentrations of d-amino acid hydroxamates and acyl hydroxamates that have previously been shown to be powerful inhibitors of this enzyme (wilkes, s. h., and prescott, j. m. (1983) j. biol. chem. 258, 13517-13521). d-valine hydroxamate produces the most distinctive perturbation, splitting the characteristic 527 nm absorption peak of the cobalt enzyme to form peaks at 564 ...19873597389
[aeromonas hydrophila as an autochthonous causative agent of infectious enteritis in germany].aeromonas hydrophila was identified by culture technique in diarrhoeal stools and ascites in a patient of 57 years of age who suffered from known alcoholic cirrhosis and additional acute gastroenteritis. no other pathogens that would produce acute diarrhoea were found. the isolated aeromonas hydrophila strain was characterised by the pathogenic properties known to date as pertaining to this species (formation of haemolysin and enterotoxins). hence it is evident that aeromonas hydrophila occurs i ...19873608836
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.19873611325
pyomyositis and hepatic abscess in association with aeromonas hydrophila sepsis.this report describes a patient with chronic liver disease in whom aeromonas hydrophila sepsis developed following ingestion of fresh lake water. a hepatic abscess and bilateral calf pyomyositis, an extremely rare and previously fatal complication of a. hydrophila septicemia, subsequently developed. the patient recovered following bilateral fasciotomies and systemic antibiotic therapy with gentamicin and trimethoprim/sulfamethoxazole. no previous survivors of a. hydrophila pyomyositis secondary ...19873618636
enlarging erythematous macule: a cutaneous sign for fatal aeromonas hydrophila septicemia. 19873625077
detection of fish antibody against protein antigen of aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system.antibody against aeromonas salmonicida was detected in sera from immunised or experimentally infected rainbow trout by enzyme-linked immunosorbent assay (elisa) using the biotin-avidin system. the elisa titre correlated well with the agglutinin titres of the sera, but the elisa was found to be more sensitive than the agglutination test. when the rainbow trout serum was separated by column chromatography, antibody activity (determined by elisa and agglutination test) was detected in the igm fract ...19873628989
bacteriology of the freshwater environment: implications for clinical therapy.water and animal tissue samples were obtained from sources in tennessee, california, and florida. purified bacterial colonies were isolated and organisms identified. fifty-eight isolates were recovered. twenty-seven gram-negative isolates were identified. gram-positive organisms were of the coryneform group or bacillus species. antibiotic susceptibility testing showed that aeromonas species were relatively resistant to a wide variety of antimicrobials, which included trimethoprim, cefazolin, and ...19873631666
[vibrio and aeromonad sensitivity to the vibriostatic o 129].sensitivity of vibrios and aeromonads to vibriostatic o129 (2,4-diamino-6,7-diisopropyl pteridine) was studied. the vibrios and aeromonads were isolated in the ussr. the tests were performed with disks made in the ussr and great britain. the content of the drug in the disks was 10 and 150 micrograms. it was shown that v. cholerae o1 v. cholerae not o1 and v. albensis were highly sensitive to vibriostatic o129 and produced no growth in the presence of either low or high concentrations of the drug ...19873631937
class d tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida.tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida were classified as class d by their expression of resistance to tetracycline and minocycline and through their dna structure.19873631946
plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates.the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics.19873631947
aeromonas hydrophila-associated colitis in a male homosexual.a 37-year-old homosexual man was evaluated for a one-week history of hematochezia. results of a physical examination were remarkable only for grossly bloody stool. sigmoidoscopy to 30 cm showed a friable mucosa compatible with an acute colitis, and a rectal biopsy specimen demonstrated an increased plasma cell infiltrate. stool cultures subsequently yielded aeromonas hydrophila; serum human t-cell lymphotropic virus type iii antibody titer was positive. the patient responded to a course of treat ...19873632156
in vitro and in vivo study of an antimicrobial activity displayed by the redmouth disease agent, yersinia ruckeri.a soluble antimicrobial product has been detected in yersinia ruckeri cultures. the contract droplets technique and the double agar layer method allowed to assess its bacteriostatic effect and the high susceptibility of other bacterial fish pathogens like vibrio and aeromonas. but it did not inhibit other strains of y ruckeri. no correlation was found between the antimicrobial activity and the virulence of the strains. as mixed infections with a salmonicida and y ruckeri could be obtained experi ...19873579181
nucleotide sequence of the gene for the hole-forming toxin aerolysin of aeromonas hydrophila.the gene for the hole-forming toxin aerolysin from aeromonas hydrophila was sequenced. although most of the sequence seems unrelated to that of staphylococcus aureus alpha-toxin, both proteins are very hydrophilic, and they each contain a nearly identical string of 10 amino acids.19873584074
congo red uptake by motile aeromonas species.virulence of several species of enteropathogenic bacteria has been correlated with the ability of isolates to take up the dye congo red. to determine whether congo red uptake might be a useful marker for virulence of motile aeromonas species, we examined 50 strains of diverse clinical origin on a medium containing 50 micrograms of congo red per ml. all of the strains took up the dye to various degrees. for most strains, uptake was greatest at 37 degrees c and least at 22 degrees c. production of ...19873584422
aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea.in 1983, the vernacular name enteric group 77 was coined for a group of strains that had been referred to our laboratory as "possible vibrio cholerae except for gas production." by dna-dna hybridization (hydroxyapatite, 32p), 8 of 10 strains of enteric group 77 were very highly related to the labeled strain 1169-83 (74 to 100% at 60 degrees c and 75 to 100% at 75 degrees c; percent divergence, 0.0 to 2.5). type strains of six other aeromonas species were 45 to 66% related (60 degrees c) to strai ...19873584425
clinical and biochemical significance of toxin production by aeromonas hydrophila.production of cytotoxin and enterotoxin by aeromonas strains obtained from stools of 50 children in mexico and texas and from blood of 9 children with sepsis was determined. results were correlated with clinical features of infected children as well as with biochemical traits of aeromonas strains. cytotoxin was produced by 40 of 42 aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsi ...19873584426
growth of mixed microbial populations in ground water containing highly chlorinated organic wastes. 19873567405
[septicemia caused by aeromonas hydrophila]. 19873567701
isolation frequency of aeromonas species in relation to patient age.over a period of 18 months aeromonas spp. were isolated from stool specimens of 116 patients (total isolation frequency 1.6%). isolation rates in children up to ten years of age were significantly higher only for aeromonas caviae as compared to other age groups. additional data are needed to assess the clinical significance to these findings.19873569253
research on aeromonas and plesiomonas. ii. non-gastrointestinal diseases. 19873569483
research on aeromonas and plesiomonas. iii. enteric infections. 19873569484
identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish.ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ...19873555336
epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases.an epidemiology analysis of a series of 12 aeromonas hydrophila infections, including six of septicaemia, which occurred on several wards of one hospital during the summer of 1982 is presented. the hypothesis that the hospital water could be the source of these infections was supported by the isolation of 1-10 motile aeromonads per ml in most of the water samples collected from various points on the hospital water system. electrophoretic esterase typing was used as an epidemiological screening m ...19873556437
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...19873560188
enumeration and characterization of aeromonas hydrophila and aeromonas caviae isolated from grocery store produce.starch-ampicillin agar was used to quantitatively isolate aeromonas sp. from retail grocery store produce. all produce sampled, including parsley, spinach, celery, alfalfa sprouts, broccoli, and lettuce, contained aeromonas sp. in most instances, the count of aeromonas sp. increased 10- to 1,000-fold during 2 weeks of storage at 5 degrees c. eleven (92%) of 12 kinds of produce yielded cytotoxic aeromonas sp. identification as aeromonas hydrophila was the strongest indicator of cytotoxicity, and ...19873566266
[properties of aeromonas hydrophila enterotoxins from a food hygiene viewpoint]. 19873566431
research on aeromonas and plesiomonas. iv. virulence factors. 19873552716
binding of clavulanic acid to the penicillin-binding proteins of aeromonas hydrophila. 19873509501
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...19873496845
[fatal aeromonas septicemia in two patients with acute myelogenous leukemia: clinical significance in neutropenic states and review of the literature]. 19873323564
evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.a small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of enterobacteriaceae, 15 strains of aeromonas sp., and 3 strains of plesiomonas shigelloides. the system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. ninety strains (18.6%) were not identified. only 11 str ...19873326369
phenotypic differentiation of members of the family vibrionaceae using miniaturized biochemical tests.enzymatic hydrolysis of 27 different chromogenic substrates and the assimilation of 44 carbon sources by 144 strains of vibrio species of clinical importance, aeromonas hydrophila and plesiomonas shigelloides were studied by standardized micromethods. some classical biochemical tests were also performed using the test kit tte-as (flow laboratories gmbh, meckenheim, frg). reading of results was done automatically by a photometer and test data were recorded and stored by a microcomputer. all speci ...19873326370
[a case of aeromonas hydrophila sepsis with generalized gas-formation and intravascular hemolysis]. 19873334425
intestinal infections with aeromonas. 19873544999
in vitro activity of ci-934 compared with ciprofloxacin, enoxacin, norfloxacin, and vancomycin.the in vitro activity of the quinolone ci-934 was compared with ciprofloxacin, norfloxacin, enoxacin, and vancomycin against 607 gram-positive and -negative isolates. ci-934 inhibited 90% of the enterobacteriaceae, aeromonas hydrophila, and acinetobacter spp. at 1 microgram/ml. decreased activity was observed against pseudomonas aeruginosa. pseudomonas maltophilia, and other pseudomonas spp. with ci-934 mic90 greater than or equal to 8 micrograms/ml. ci-934 activity against gram-positive organis ...19873472704
cutaneous infections of the coast. 19873480441
the effect of different brands of macconkey and xylose lysine deoxycholate agar on the isolation of aeromonas hydrophila.aeromonas hydrophila has been reported to be involved in the opportunistic infection of human beings, therefore, its rapid diagnosis from the clinical specimens was considered to be extremely important for the prognosis. the effect of different brands of macconkey (mac) and xylose lysine deoxycholate (xld) agars on the plating efficiency and colony size of a. hydrophila was evaluated by comparing with blood agar. results indicate that (i) except eiken's mac have low plating efficiency, all the m ...19873428016
enteric pathogens in tropical aquaria.a total of 100 tropical aquaria from six pet shops were sampled for the presence of salmonella, campylobacter and aeromonas species. eight fish tanks contained unusual serotypes of salmonella. none had been used to house terrapins. one hundred and three isolates of aeromonas species were obtained from 98 tanks. one tank contained plesiomonas shigelloides. no campylobacter species were isolated. it is suggested that a tropical aquarium may constitute an infection hazard.19873428370
lymphadenopathy syndrome and hiv infection in multitransfused beta-thalassemia child.lymphoadenopathic syndrome (las), diagnosed also with histologycal studies, is described in a polytransfused 11-year old boy affected by beta-thalassemia major. after three days of the admission he suffered a serious acute diarrhea by aeromonas hydrophila, an opportunistic enteric bacteria demonstrated, up to date, in immunocompromised patients and never in aids patients. it is important to remark that beta-thalassemic patients are at high risk from hiv infections, either for the chronic need of ...19873435653
[spontaneous bacterial peritonitis caused by aeromonas hydrophila. presentation of 2 cases]. 19873438545
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
[geographic variations of the isolation of aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population]. 19873444950
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
[pulmonary abscess: aeromonas hydrophila caused by a complication of drowning in fresh water]. 19873450216
are medicinal leeches an infection hazard? 19873153606
[hospital infections in relation to the use of leeches]. 19872954135
[aeromonas hydrophila infection of the scalp. peculiarities]. 19872955380
a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures.aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ...19873298187
in vitro activity and beta-lactamase stability of a new monobactam, b0-1165.b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ...19873300528
[study of the sensitivity of enteropathogenic bacteria to nifurzide].nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ...19873302859
marker exchange mutagenesis of the aerolysin determinant in aeromonas hydrophila demonstrates the role of aerolysin in a. hydrophila-associated systemic infections.we report here on the isolation of isogenic strains of aeromonas hydrophila ab3 deleted for a segment of the aerolysin gene. all aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for chinese hamster ovary tissue culture cells. one such mutant, ab3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of a. hydrophila infections. the strain had a 50% lethal dose (ld50) of great ...19873305370
marine bacteria which produce tetrodotoxin.a number of type strains of marine bacteria, including members of the family vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. most of the vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both.19873310884
natural microbiologic hazards of the aquatic environment. 19873311341
[tte-ras (titertek-enterobac-ras), a new micromethod for the semi-automatic identification of enterobacteriaceae within 5 hours].titertek-enterobac-ras (tte-ras), a new semi-automated system for the identification of the enterobacteriaceae-within five hours, has been evaluated and compared with conventional methods. this note presents the results upon 655 strains, mainly enterobacteriaceae tte-ras provided correct identification for about 92 p. cent and 97 p. cent of enterobacteriaceae respectively before and after carrying out supplementary tests according to manufacturer's instruction. tte-ras gave 97 p. cent specific r ...19873314607
[aeromonas hydrophila as the causative agent of ileocolitis]. 19873315547
[aeromonas spp. as pathogens in human infections: diagnosis and clinical significance].aeromonads have been described as causative agents in a wide variety of infections in immunosuppressed as well as in immunocompetent patients. it is still controversial whether or not some or all three currently recognized species have to be considered as enteropathogenic. clinical and epidemiological data are incomplete and contradictory although laboratory diagnosis does not cause real problems.19873315971
does aeromonas hydrophila preferentially colonize the bowels of patients with hematologic malignancies?weekly cultures of stools from neutropenic patients and bone marrow transplant recipients yielded aeromonas hydrophila from 8% of 88 patients over a 2-yr period. during this time stools in the routine enteric laboratory yielded a. hydrophila in 0.24% of 1632 patients. although the patient groups and culture methods were not directly comparable, this significant difference in isolation rate (p less than 0.001) may reflect a higher colonization rate in the immunocompromised patient.19873319373
immunoglobulin binding by the regular surface array of aeromonas salmonicida.the cell surface of aeromonas salmonicida is covered by a regular surface array composed of a single species of protein, the a-protein (phipps, b. m., trust, t. j., ishiguro, e. e., and kay, w. w. (1983) biochemistry 22, 2934-2939). the array, known as the a-layer, is the key virulence factor for this organism. cells containing the a-layer specifically bound rabbit igg and human igm with high affinity (kd = 1.0 x 10(-6) m and 3.3 x 10(-6) m, respectively), but neither isogenic a-protein-deficien ...19882967820
virulence properties and enterotoxin production of aeromonas strains isolated from fish.the biological activities in vivo and in vitro of 59 motile aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. a total of 64.3% of the isolates tested were pathogenic for fish, and 62% of aeromonas hydrophila and a. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. although the majority of the strains were proteolytic and amylolytic and produced dnase, other activities, such as elastase and staph ...19882972627
aeromonas-associated gastroenteritis in children.during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...19883340460
aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite. 19883340462
effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains.motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains.19883343335
production of monoclonal antibody against aeromonas hydrophila haemolysin.two hybridoma cell lines that produce monoclonal antibodies against aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. enzyme-linked immunosorbent assay (elisa) showed that the two purified monoclonal antibodies, b7 and b11, recognised the same epitope on the haemolysin molecule. antibody b7 neutralised the haemolytic and enterotoxic activities of the haemolysin. it is concluded ...19883346901
[prevalence of aeromonas spp. pediatric gastroenteritis].a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ...19883355951
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...19883366862
structure of an s layer on a pathogenic strain of aeromonas hydrophila.negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ...19883372478
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