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bacterial symbionts in the hepatopancreas of isopods: diversity and environmental transmission.the midgut glands (hepatopancreas) of terrestrial isopods contain bacterial symbionts. we analysed the phylogenetic diversity of hepatopancreatic bacteria in isopod species from various suborders colonizing marine, semiterrestrial, terrestrial and freshwater habitats. hepatopancreatic bacteria were absent in the marine isopod idotea balthica (valvifera). the symbiotic bacteria present in the midgut glands of the freshwater isopod asellus aquaticus (asellota) were closely related to members of th ...200717506824
interaction between innate immune cells and a bacterial type iii secretion system in mutualistic and pathogenic associations.animals house a community of bacterial symbionts in their digestive tracts that contribute to their well being. the medicinal leech, hirudo verbana, has a remarkably simple gut population carrying two extracellular microbes in the crop where the ingested blood is stored. this simplicity renders it attractive for studying colonization factors. aeromonas veronii, one of the leech symbionts, can be genetically manipulated and is a pathogen of mammals. screening transposon mutants of a. veronii for ...200717517651
aeromonas infection in critically ill burn patients.aeromonas infection in burn patients is extremely uncommon. here we report on four cases of aeromonas infection in burn patients admitted to the bicu at the singapore general hospital burn unit between june 2001 and may 2006. two patients had positive blood cultures, and the other two had tissue samples with growth. there was no history of exposure to soil or fresh water in all patients. the average age of patients was 35 years (range 24-41) and the average % tbsa was 48% (range 35-80). cultures ...200717521817
cystitis caused by aeromonas caviae.aeromonas sp. organisms rarely cause urinary tract infection. we report for the first time a case of urinary tract infection caused by a. caviae in an adult patient with a history of increased frequency of urination, dysuria, hematuria, and weight loss.200717522269
antibiotic and heavy metal resistance in motile aeromonads and pseudomonads from rainbow trout (oncorhynchus mykiss) farms in australia.a total of 129 pseudomonas spp. and 90 aeromonas spp. were isolated from nine rainbow trout (oncorhynchus mykiss) farms in australia. all the isolates were tested for sensitivity to 15 antibiotics and the multiresistant strains were tested for sensitivity to seven heavy metals. minimal inhibitory concentrations (mics) were determined by the agar dilution method. in pseudomonas spp., resistance to amoxicillin, cefalothin, ceftiofur, ticarcillin, chloramphenicol, florfenicol, streptomycin, nitrofu ...200717524624
characterization of a catalase gene from aeromonas veronii, the digestive-tract symbiont of the medicinal leech.the catalase gene kata of the medicinal leech symbiont aeromonas veronii bv. sobria was cloned, sequenced, and functionally characterized. southern hybridization, using an a. veronii kata-specific hybridization probe, suggested the presence of a single gene copy in many aeromonas species. a. veronii kata consisted of 1446 nt encoding a protein with a high degree of similarity to the small-subunit group iii bacterial catalases. a catalase-null mutant (jg186) was constructed through gene-replaceme ...200717526846
site-directed mutagenesis of aeromonas hydrophila enoyl coenzyme a hydratase enhancing 3-hydroxyhexanoate fractions of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).the aim of this study is to enhance 3-hydroxyhexanoate (3hhx) fractions of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), abbreviated as phbhhx, through site-directed mutagenesis of aeromonas hydrophila enoyl coenzyme a hydratase (phaj(ah)). two amino acids (leu-65 and val-130) were selected as a substitutional site based on the structural information of phaj(ah). the purified proteins from the wild-type enzyme and mutants were used to determine hydratase activities. hydratase activities of four ...200717534562
characterization of a broad range antibacterial substance from a new bacillus species isolated from amazon basin.a bacillus sp. strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. the phylogenetic analysis indicated that this strain has low sequence similarity with most bacillus spp., suggesting a new species was isolated. the antimicrobial activity was detected starting at the exponential growth phase, and maximum activity was observed at stationary phase. the substance was inhibitory to a broad range of indicator strains, including pathogenic ...200717534601
molecular cloning and sequence analysis of lamb encoding outer membrane maltose-inducible porin of aeromonas hydrophila.aeromonas hydrophila is a significantly important pathogen causing major diseases in humans and fresh water fish. the outer membrane proteins (omp) which are strong immunogens have been reported to act as adhesins aiding in the attachment of enteropathogenic bacteria. it is of interest to investigate the role of omp in pathogenesis and their potential as vaccine candidates. in our laboratory, we cloned the gene encoding channel protein lamb porin of a. hydrophila. dna sequence analysis revealed ...200717541836
variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of aeromonas caviae pha synthase.amino acid substitutions at two residues downstream from the active-site histidine of polyhydroxyalkanoate (pha) synthases are effective for changing the composition and the molecular weight of pha. in this study, saturation mutagenesis at the position ala505 was applied to pha synthase (phacac) from aeromonas caviae to investigate the effects on the composition and the molecular weight of pha synthesized in ralstonia eutropha. the copolymer composition and molecular weight of pha were varied by ...200717541931
filter-feeder macroinvertebrates as key players in culturable bacteria biodiversity control: a case of study with sabella spallanzanii (polychaeta: sabellidae).the present study investigates the effect of the filtering activity of sabella spallanzanii on the culturable heterotrophic bacterial community through the comparison of the bacterial diversity in transplanted polychaetes and the surrounding seawater. for isolation of culturable heterotrophic bacteria, seawater samples as well as polychaete homogenates were plated in triplicates onto bacto marine agar 2216 (difco). all the colonies grown were isolated, subcultured and identified by several morph ...200717548107
use of the novel phylogenetic marker dnaj and dna-dna hybridization to clarify interrelationships within the genus aeromonas.the interrelationships of 27 aeromonas strains were investigated using dnaj sequences and dna-dna hybridization. dnaj sequence similarities showed a stronger relationship with dna-dna relatedness values than did 16s rrna gene sequence similarities. additionally, dnaj sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16s rrna gene sequences for the differentiation of strains at the species level. relationships among aeromonas species were theref ...200717551035
bacteriostatic effect of flavonoids isolated from leaves of psidium guajava on fish pathogens.the antimicrobial activity against fish bacterial pathogens of flavonoids (morin, morin-3-o-lyxoside, morin-3-o-arabinoside, quercetin, and quercetin-3-o-arabinoside) isolated from the leaves of psidium guajava was evaluated. the flavonoids were shown to have bacteriostatic effect on all of the tested bacteria.200717553634
biochemical and functional characterization of udp-galactose 4-epimerase from aeromonas hydrophila.bacteria of genus aeromonas, responsible for a variety of pathological conditions in humans and fish, are ubiquitous waterborne bacteria. aeromonas produces several virulent factors including a complex of lipopolysaccharide and surface array protein, involved in colonization. udp-galactose 4-epimerase (gale) catalyzes the production of udp-galactose, a precursor for lipopolysaccharide biosynthesis, and thus is an important drug target. gale exhibits interspecies variation and heterogeneity at it ...200717553760
[isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba].extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ...200717554439
characterization of aeromonas caviae and a. veronii by standardized cellular protein electrophoretic patterns.aeromonas caviae and a. veronii recovered from clinical and environmental samples were characterized by sds-page of whole cell proteins. all strains were typable and showed unique banding patterns. a high intra-specific diversity within these two microorganisms was revealed. separation of the polish strains from the hong-kong isolates was observed. a. caviae and a. veronii were not distinguishable with respect to their ecological origin. however, strains with very similar protein patterns could ...200717571798
the role of c-di-gmp signaling in an aeromonas veronii biovar sobria strain.aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. it is shown that all isolates of persistent aeromonas clones show strong biofilm formation ability. c-di-gmp regulates biofilm formation in many bacteria. to investigate the impact of c-di-gmp signaling, we introduced heterologous ggdef and eal domain proteins from salmonella typhimurium to an aeromonas veronii biovar sobria strain. overexpression of the ggdef domain protein adra increased c-di-gmp concentration ...200717573931
x-ray crystallographic characterization of the co(ii)-substituted tris-bound form of the aminopeptidase from aeromonas proteolytica.the x-ray crystal structure of the co(ii)-loaded form of the aminopeptidase from aeromonas proteolytica ([coco(aap)]) was solved to 2.2a resolution. [coco(aap)] folds into an alpha/beta globular domain with a twisted beta-sheet hydrophobic core sandwiched between alpha-helices, identical to [znzn(aap)]. co(ii) binding to aap does not introduce any major conformational changes to the overall protein structure and the amino acid residues ligated to the dicobalt(ii) cluster in [coco(aap)] are the s ...200717574677
controversial data on the association of aeromonas with diarrhoea in a recent hong kong study. 200717577068
[cellulitis caused by aeromonas hydrophila in an immunocompromised old woman]. 200718232124
comparison of the bactec blood culture system versus conventional methods for culture of normally sterile body fluids.this study compared the bactec blood culture system (becton dickinson diagnostic instrument systems, sparks, md) with conventional culture methods for recovery and time to detection of significant isolates from normally sterile body fluids. a total of 412 specimens were included in the study. half of the specimens were inoculated directly into the automated blood culture system. the remaining specimens were centrifuged at 3000 rpm for 10 min and were inoculated onto conventional media. clinicall ...200718165209
the involvement of teta and tete tetracycline resistance genes in plasmid and chromosomal resistance of aeromonas in brazilian strains.this study analyzed the involvement of teta and tete genes in the tetracycline resistance of 16 strains of genus aeromonas, isolated from clinical and food sources. polymerase chain reactions revealed that 37.5% of the samples were positive for teta, and also 37.5% were tete positive. one isolate was positive for both genes. only the isolate a. caviae 5.2 had its resistance associated to the presence of a plasmid, pss2. the molecular characterization of pss2 involved the construction of its rest ...200718060315
isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario.for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ...200717984256
aeromonas hydrophila wound infection following a tiger bite in nepal.aeromonas hydrophila is a rare human pathogen. reports of zoonotic infection developing after large feline bites are even rarer. we are documenting the first case of human wound infection with a. hydrophila following a tiger bite. the patient responded well following wound debridement, secondary suturing and combination antibiotic therapy.200718041304
antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria.the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ...200718041957
arsenic-resistant bacteria isolated from contaminated sediments of the orbetello lagoon, italy, and their characterization.the aim of this study was to isolate arsenic-resistant bacteria from contaminated sediment of the orbetello lagoon, italy, to characterize isolates for as(iii), as(v), heavy metals resistance, and from the phylogenetic point of view.200718045414
isolation of a novel strain of aeromonas media producing high levels of dopa-melanin and assessment of the photoprotective role of the melanin in bioinsecticide applications.to isolate a bacterium that produces high yield of melanin and to examine the effect of this bacterial pigment on the efficacy of a bioinsecticide.200718045437
accumulation of silver(i) ion and diamine silver complex by aeromonas sh10 biomass.the biomass of aeromonas sh10 was proven to strongly absorb ag+ and [ag(nh3)2]+. the maximum uptake of [ag(nh3)2]+ was 0.23 g(ag) g(-1)(cell dry weight), higher than that of ag+. fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. after sh10 cells were suspended in the aqueous solution of [ag(nh3)2]+ under 60 degrees c for more than 12 h, [ag(nh3)2 ...200718025596
combination of n149s and d171g mutations in aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis.aeromonas caviae polyhydroxyalkanoate synthase (phac(ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. in a previous study, two phac(ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (n149s) or aspartate 171 by glycine (d171g) were isolated as higher active enzymes by means of evolutionary engineering. in this study, ...200718031343
aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption.prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ...200718032845
a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria.aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america.200717999586
[neutropenic enterocolitis in adult patients with acute leukemia or stem cell transplant recipients: study of 7 cases].neutropenic enterocolitis (ne) is a complication arising in neutropenic patients with acute leukemia or solid tumours while treated with intensive chemotherapy. the optimal therapeutic procedures have not been well established.200718005634
[preparation and characterization of the mab against vibrio harveyi].to prepare the mab against vibrio harveyi and analyse their biological properties.200717825231
cloning, expression and characterization of aerolysin from aeromonas hydrophila in escherichia coli.aerolysin is a toxin (protein in nature) secreted by the strains of aeromonas spp. and plays an important role in the virulence of aeromonas strains. it has also found several applications such as for detection of glycosylphosphatidylinositol (gpi)-anchored proteins etc. a. hydrophila is a ubiquitous gram-negative bacterium which causes frequent harm to the aquaculture. to obtain a significant amount of recombinant aerolysin in the active form, in this study, we expressed the aerolysin in e. col ...200717970277
febrile response to infection in the american alligator (alligator mississippiensis).temperature probes were inserted into the stomachs of juvenile american alligators (alligator mississippiensis) maintained outdoors at ambient fluctuating temperatures. internal body temperatures (t(b)) were measured every 15 min for two days, and then the alligators were injected with bacterial lipopolysaccharide (lps), pyrogen-free saline, or left untreated. alligators injected intraperitoneally with lps exhibited maximum t(b)s 2.6+/-1.1 degrees c and 3.5+/-1.2 degrees c higher than untreated ...200717977038
carbohydrate analysis and serological classification of typical and atypical isolates of aeromonas salmonicida: a rationale for the lipopolysaccharide-based classification of a. salmonicida.the cell envelope of aeromonas salmonicida contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of bacterial cell membrane. using a recently developed in-source fragmentation technique, we screened 39 typical and atypical isolates of a. salmonicida and established their o-chain polysaccharide structure by capillary electrophoresis-mass spectrometry (ce-ms), compositional and linkage analyses and comparison to the previously determined o-chain polysaccharide st ...200717658271
mesophilic aeromonas udp-glucose pyrophosphorylase (galu) mutants show two types of lipopolysaccharide structures and reduced virulence.a mutation in galu that causes the lack of o34-antigen lipopolysaccharide (lps) in aeromonas hydrophila strain ah-3 was identified. it was proved that a. hydrophila galu is a udp-glucose pyrophosphorylase responsible for synthesis of udp-glucose from glucose 1-phosphate and utp. the galu mutant from this strain showed two types of lps structures, represented by two bands on lps gels. the first one (slow-migrating band in gels) corresponds to a rough strain having the complete core, with two sign ...200717660404
prevalence of bacterial pathogens in biofilms of drinking water distribution systems.water for human consumption is required to be free from any bacteria that might pose a health risk. the presence of biofilms in the drinking water distribution system may play a role in the presence of potential pathogens in the drinking water supply. ninety-five biofilm samples from various parts of south africa were tested for the presence of escherichia coli, aeromonas, pseudomonas, salmonella, shigella and vibrio spp. members of these genera were quantified by the three-tube most probable nu ...200717674571
a comparison of ten usepa approved total coliform/e. coli tests.since 2002, the united states environmental protection agency (usepa) has approved ten enzyme-based total coliform and e. coli detection tests for examination of drinking water. these tests include: colilert, colilert-18, colisure, m-coli blue 24, readycult coliforms 100, chromocult, coliscan, e * colite, colitag and mi agar. the utility of the enzyme based test systems is based on both the ability of the test to detect the target organisms at low levels and the ability of the test system to sup ...200717674575
haemato-immunological responses to dietary yeast rna, omega-3 fatty acid and beta-carotene in catla catla juveniles.a preliminary study with 60 days feeding was conducted to study the immunomodulatory role of different immunostimulants like beta-carotene, omega-3 fatty acid and yeast-rna in catla catla fingerlings. two hundred and sixty four fingerlings were randomly distributed into eight treatment groups with each of three replicates. eight isonitrogenous (crude protein 34.12-35.40%) and isocaloric (458.41-461.48 kcal/100g) purified diets were prepared with graded level of beta-carotene, omega-3 fatty acid ...200717681812
first occurrence of an imp metallo-beta-lactamase in aeromonas caviae: imp-19 in an isolate from france.we describe the first imp metallo-beta-lactamase in aeromonas caviae: imp-19, which differed from imp-2 by a single amino acid change (arg to ala at position 38). bla(imp-19) was found within a class 1 integron located on a 35-kb plasmid. this is also the first description of an imp producer in france.200717938180
screening and characterization of a bioflocculant produced by aeromonas sp.to isolate the bioflocculant-producing bacteria from activated sludge and investigate the flocculating characteristics of the newly isolated bioflocculant.200717948760
prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, río tinto (sw, spain).the prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, río tinto (sw, spain), has been analysed through denaturing gradient gel electrophoresis (dgge), cloning of 16s rrna genes and fluorescence in situ hybridization (fish). the dominant species present in the macrofilaments were acidithiobacillus ferrooxidans, leptospirillum ferrooxidans and acidiphilium spp., which represented the three main bacterial genera found in the water column o ...200717950555
salt in surroundings influences the production of serine protease into milieu by aeromonas sobria.previously we have shown that the open reading frame 2 protein (orf2 protein), which is encoded at the 3 ' end of serine protease of aeromonas sobria (asp), functions as a chaperone protein in periplasm in the production of asp. both proteins, asp and orf2 protein, associate in periplasm and orf2 protein helps asp to take an active form. asp which is dissociated from orf2 protein emerges in milieu . in this study, we examined the effect of sodium chloride (nacl) in medium on asp production by a. ...200717951986
isolation and selection of bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish.to isolate, select and evaluate bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish.200717953558
bacillus subtilis ab1 controls aeromonas infection in rainbow trout (oncorhynchus mykiss, walbaum).to develop a probiotic with effectiveness against aeromonas sp., which was pathogenic to rainbow trout.200717953580
luxri homologs are universally present in the genus aeromonas.aeromonas spp. have been regarded as "emerging pathogens". aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a phenomenon that might be regulated by quorum sensing. however, only two species of the genus are reported to possess the luxri quorum sensing gene homologs. the purpose of this study was to investigate if the luxri homologs are universally present in the aeromonas strains collected from various cu ...200717953777
cloning and characterization of photobacterium damselae ssp. piscicida phospholipase: an enzyme that shows haemolytic activity.a phospholipase gene of photobacterium damselae ssp. piscicida (ppp) was cloned from a genomic library and its nucleotide sequence was determined. the open reading frame consisted of 1218 bp encoding a protein of 405 amino acids with a predicted molecular mass of 46 kda. the ppp had identities (53-55%) with phospholipase and haemolysin of vibrio spp., while it showed low identities (23-26%) with glycerophospholipid cholesterol acyltransferase of aeromonas spp. a recombinant ppp (rppp) with a his ...200717958612
analysis of type iii effector genes in typical and atypical aeromonas salmonicida. 200717958615
tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning.a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ...200717965361
aeromonas hydrophila infection causing delayed vascular thrombosis in a nearly amputated finger.we discuss an unusual case of aeromonas hydrophila infection in a partially amputated digit from contaminated animal products resulting in delayed vascular thrombosis and amputation.200717826563
rapid detection of virulence factors of aeromonas isolated from a trout farm by hexaplex-pcr.the detection of virulence factors of aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. in this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and aeromonas species were isolated and identified. for rapid detection of six virulence factors of isolated aeromonas, a hexaplex-polymerase chain reaction (hexaplex-pcr) assay was used. the detected virulence factors include a ...200717846582
inactivation of pseudomonas aeruginosa and aeromonas hydrophila by silver in tap water.this study was conducted to assess the efficacy of silver as a secondary disinfectant to replace or reduce the level of chlorine utilized in water distribution systems. pseudomonas aeruginosa and aeromonas hydrophila are opportunistic pathogens present in drinking water and have been associated with waterborne disease. after 8 hours of exposure to 100 microg/l of silver, there was a >6-log10 reduction in p. aeruginosa in tap water at room temperature at ph7 and a 5.55-log10 reduction in the pres ...200717849299
the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum).jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ...200717850573
molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein.lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ...200717850883
prevalence of campylobacter jejuni and enteric bacterial pathogens among hospitalized hiv infected versus non-hiv infected patients with diarrhoea in southern india.a prevalence study on campylobacter jejuni and other enteric bacterial pathogens was carried out in 200 hiv infected and 200 non-hiv infected subjects with diarrhoeal symptoms at an aids hospital in southern india. diarrhoeal specimens were inoculated onto standard culture media as well as onto columbia and campylobacter blood agar media for c. jejuni isolation. all the c. jejuni isolates were tested for antimicrobial susceptibility using kirby-bauer's method. a significant difference in recover ...200717852888
phenoloxidase is an important component of the defense against aeromonas hydrophila infection in a crustacean, pacifastacus leniusculus.the melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, aeromonas hydrophila, in the freshwater crayfish, pacifastacus leniusculus. rna interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phago ...200717855335
identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16s rrna gene sequencing.bacteria present in water samples taken on a weekly basis, from june 2004 through june 2005, from three streams, were cultured on coliscan easygel agar plates. colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16s rrna gene. a total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. of 175 dark blue/purple colonies presumed to be e. coli, sequence analysis indicated that 45 ...200717878554
genetic variations in aeromonas hydrophila isolates from clinical and environmental sources in thailand.aeromonas hydrophila, a widely distributed human pathogen causing a variety of diseases, can be isolated from clinical and environmental sources. analysis in thailand of 110 isolates of aeromonas hydrophila by randomly amplified polymorphic dna-pcr (rapd-pcr) revealed one specific rapd pattern group (g) that was associated only with strains from environmental sources. cytotoxic activity, adhesion to epithelial cells and exoenzyme secretions of a. hydrophila were also investigated. a comparison o ...200717883013
disruption of the polyhydroxyalkanoate synthase gene in aeromonas hydrophila reduces its survival ability under stress conditions.aeromonas hydrophila 4ak4 produces phbhhx copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate. in this paper, the physiological functions of phbhhx on the bacterial survival ability were systematically studied using a phbhhx synthase phac-disrupted mutant termed a. hydrophila cq4 and its wild type a. hydrophila 4ak4. it was found that phbhhx synthesis in a. hydrophila 4ak4 provided improved resistance against environmental stress factors including heat and cold treatments, hydrog ...200717888005
evaluating virulence of waterborne and clinical aeromonas isolates using gene expression and mortality in neonatal mice followed by assessing cell culture's ability to predict virulence based on transcriptional response.to assess the virulence of aeromonas spp. using two models, a neonatal mouse assay and a mouse intestinal cell culture.200717897183
interactions of clinical and environmental aeromonas isolates with caco-2 and ht29 intestinal epithelial cells.evaluation of adherence and invasion of aeromonas spp. to human colon carcinoma cell lines caco-2 and ht29 and assessment of cytotoxic activity.200717897383
[microbiological characteristics of spontaneous bacterial peritonitis in korea]. 200717898547
[changes in the profiles of causative agents and antibiotic resistance rate for spontaneous bacterial peritonitis: an analysis of cultured microorganisms in recent 12 years].the causative agents for spontaneous bacterial peritonitis (sbp) and antibiotic resistance rate vary according to the regions and time. this study evaluated the recent changes in the profiles of microorganisms and antibiotic resistance rate for the choice of effective antibiotics in treating sbp.200717898553
virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study.a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ...200717898843
molecular characterization of class 1 integrons and antimicrobial resistance in aeromonas strains from foodborne outbreak-suspect samples and environmental sources in taiwan.one hundred thirty-three aeromonas spp. isolates were examined for multiple antibiotic resistance phenotypes and prevalence of class 1 integron sequences. twenty-four (18.0%) of these isolates contained class 1 integron. seven different class 1 integrons were found among 24strains, with a total of 10 different gene cassettes encoding for resistance to trimethoprim (dfr12 and dfr2d), aminoglycosides (aada1 and aada2), beta-lactam antibiotics (oxa2), chloramphenicol (catb3 and catb8), quaternary a ...200717908616
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant.to investigate the presence and distribution of integron-carrying bacteria from a slaughterhouse wastewater treatment plant (wwtp).200717913715
on the identification of clinical aeromonas by a new restriction fragment length polymorphism of 16s rdna method. 200717916125
viscoelastic properties of an exopolysaccharide: aeromonas gum, produced by aeromonas nichidenii 5797.the viscoelastic properties of aeromonas (a) gum in water were investigated by using the rheometric scientific ares controlled strain rheometer. an intrinsic viscosity of 8336 ml/g was obtained according to the fuoss-straus equation. the effect of salt concentration on intrinsic viscosity revealed that the a gum exists as semiflexible chain. typical shear-thinning (pseudoplastic) behavior was observed at concentrations higher than 0.52%. the zero shear viscosity (eta(0)) increased with increasin ...200718401077
prevalence, pathogenesis, antibiotic susceptibility profiles, and in-vitro activity of selected medicinal plants against aeromonas isolates from stool samples of patients in the venda region of south africa.the prevalence, pathogenic indices, such as haemolytic and haemagglutinating activities, antibiograms, and in-vitro activities of local medicinal plants against aeromonas isolates in vhembe district of limpopo province, south africa, were studied using standard microbiological methods. in total, 309 diarrhoeic stool samples were collected from patients attending five health centres in the region during december 2004-may 2005. aeromonas species were identified using the api 20e system. the haemag ...200718402186
antimicrobial activity of tissue and associated bacteria from benthic sea anemone stichodactyla haddoni against microbial pathogens.associated bacteria from stichodactyla haddoni are found maximum in tentacle tissues than the body tissue. there are eight associated bacterial species viz., alcaligenes sp, corynebacterium sp, aeromonas sp, sporosarcina sp, renibacterium sp, camobacterium sp1, camobacterium sp2 and salinococcus sp were recorded. the culture extracts from the associated bacterial species showed sensitivity against human bacterial and fungalpathogens. however, the hexane tissue extract of sea anemone showed maxim ...200718405113
quorum sensing signals are produced by aeromonas salmonicida and quorum sensing inhibitors can reduce production of a potential virulence factor.many pathogens control production of virulence factors by self-produced signals in a process called quorum sensing (qs). we demonstrate that acyl homoserine lactone (ahl) signals, which enable bacteria to express certain phenotypes in relation to cell density, are produced by a wide spectrum of aeromonas salmonicida strains. all 31 typical strains were ahl producers as were 21 of 26 atypical strains, but on a strain population basis, production of virulence factors such as protease, lipase, a-la ...200718286807
aeromonas-related diarrhoea in nasarawa, nigeria.two hundred and fifty volunteer patients attending 5 major hospitals in nasarawa town, nasarawa state, nigeria were involved in this study to determine the prevalence of aeromonas spp in persons with diarrhoea. the study population consisted of 133 males and 117 females between the ages of 0-70 years.200718240708
[identification of closely related bacteria via phylogenetic methods].to differentiate closely related pathogenic bacteria via phylogenetic method on the basis of gyrb gene sequences.200718067224
activation of prothrombin by asp, a serine protease released from aeromonas sobria.the effect of a serine protease (asp) secreted from aeromonas sobria on plasma coagulation was investigated. proteolytically active asp promoted human plasma coagulation in a dose-dependent manner. consistent with the preference for a factor xa-specific oligo-peptide substrate, asp produced enzymatic activity from human prothrombin but not from factors ix and x. asp cleaved prothrombin to produce enzymatically active 37 kda-fragment displaying the same molecular mass as alpha-thrombin. asp is th ...200718067862
[microbial diversity in scorpion intestine (buthus martensii karsch)].scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ...200718062268
the influence of selected antibiotics on aquatic microorganisms.this paper presents the influence of penicillin, erythromycin, oxytetracycline and streptomycin on aquatic microorganisms isolated from three reservoirs with varied extent of environmental pollution (sulej6w reservoir, zegrze reservoir and vistula river). the experiments were conducted in especially prepared microcosms (aerated 151 aquariums). from among the examined antibiotics streptomycin showed the longest activity in the water environment (27 days), followed by oxytetracycline (22 days). er ...200718254497
severe diarrhoea caused by aeromonas veronii biovar sobria in a patient with metastasised gist.this report describes the isolation of aeromonas veronii biovar sobria as the causative enteropathogen of diarrhoea in an oncological patient after failure of detection of other infectious agents. the case points out the severe and long course of the infection, the diagnostic dilemma, and the prompt recovery after antibiotic treatment.200718254499
role of virulence plasmid of aeromonas hydrophila in the pathogenesis of ulcerative disease syndrome in clarias batrachus.pathogenic aeromonas hydrophila (strain vb21), a multiple-drug resistance strain contains a plasmid of about 21 kb. after curing of plasmid, the isolates became sensitive to antimicrobials, to which they were earlier resistant. the cured bacteria exhibited significant alterations in their surface structure, growth profile and virulence properties, and failed to cause ulcerative disease syndrome (uds) when injected into the indian catfish clarias batrachus. routine biochemical studies revealed th ...200718341217
acute phase response in zebrafish upon aeromonas salmonicida and staphylococcus aureus infection: striking similarities and obvious differences with mammals.zebrafish has emerged as a valuable model for immunological studies. however, little is known about the overall picture of its immune response to infectious pathogens. here we present the first systematic study of its immune response to aeromonas salmonicida and staphylococcus aureus, a gram-negative and a gram-positive bacteria, respectively. genes induced upon infection were identified with suppression subtractive hybridization, with many of them encoding acute phase proteins (apps). when comp ...200716630661
clinical significance and distribution of putative virulence markers of 116 consecutive clinical aeromonas isolates in southern taiwan.the objectives of this study were to elucidate the clinical manifestations of aeromonas infections and the association of putative virulence genes with clinical invasiveness.200716716402
pancreatic abscess due to aeromonas hydrophila.aeromonas hydrophila is an uncommon cause of deep-seated infection in man. we present what we believe to be the first reported case of a pancreatic abscess due to a. hydrophila. the outcome of this case attests to the potentially lethal nature of infection that can occur with this organism.200716716404
multiple whole bacterial antigens in polyvalent vaccine may result in inhibition of specific responses in rainbow trout (oncorhynchus mykiss).the goal of fish vaccination today is to protect fish against multiple bacterial fish pathogens simultaneously using polyvalent vaccines. however, many immunological processes such as antigenic cross-reaction, antigenic competition, affinity maturation and antigen-induced suppression may affect the specificity, avidity and level of antibodies. consequently, the biological function of antibodies may be markedly different from that predicted by conventional serologic tests. here, we investigated t ...200716849036
differential expression of the pro-inflammatory cytokines il-1beta-1, tnfalpha-1 and il-8 in vaccinated pink (oncorhynchus gorbuscha) and chum (oncorhynchus keta) salmon juveniles.laboratory-reared pink and chum salmon juveniles (approximately 2g) received an intraperitoneal injection with a commercial, unadjuvanted aeromonas salmonicida bacterin or sterile saline. relative to elongation factor-1a, expression levels of genes encoding the proinflammatory cytokines interleukin-1beta-1 (il-1beta), tumour necrosis factor-alpha-1 (tnfalpha) and interleukin-8 (il-8) in pools of kidney and liver were examined 6- and 24-h after injection. expression of il-1beta was significantly ...200716926099
cloning and expression of an outer membrane protein ompts of aeromonas hydrophila and study of immunogenicity in fish.the outer membrane proteins of the warm water fish pathogen, aeromonas hydrophila have a role in the virulence of the organism and are potential candidates for vaccine development. in this study, the gene encoding an outer membrane protein designated ompts was amplified by pcr excluding the region coding for signal peptide, cloned in pqe 30-ua vector and expressed using induction with isopropyl thiogalactoside (iptg). the size of the expressed protein was 37 kda as estimated by migration in 10% ...200716959494
effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef.the effects of butylated hydroxyanisole/butylated hydroxytoluene (bha/bht), grape seed extract (activin), pine bark extract (pycnogenol), and oleoresin rosemary (herbalox) on microbial growth, color change, and lipid oxidation were investigated in cooked ground beef. when compared to the control, 1.0% activin and pycnogenol) effectively reduced the numbers of escherichia coli o157:h7 and salmonella typhimurium, and retarded the growth of listeria monocytogenes and aeromonas hydrophila. pycnogeno ...200716943089
biotransformation of isoeugenol to vanillin by pseudomonas putida ie27 cells.the ability to produce vanillin and/or vanillic acid from isoeugenol was screened using resting cells of various bacteria. the vanillin- and/or vanillic-acid-producing activities were observed in strains belonging to the genera achromobacter, aeromonas, agrobacerium, alcaligenes, arthrobacter, bacillus, micrococcus, pseudomonas, rhodobacter, and rhodococcus. strain ie27, a soil isolate showing the highest vanillin-producing activity, was identified as pseudomonas putida. we optimized the culture ...200716944125
microbial transformation of benzene to cis-3,5-cyclohexadien-1,2-diols by recombinant bacteria harboring toluene dioxygenase gene tod.toluene dioxygenase (tdo) catalyzes asymmetric cis-dihydroxylation of aromatic compounds. to achieve high efficient biotransformation of benzene to benzene cis-diols, pseudomonas putida kt2442, pseudomonas stutzeri 1317, and aeromonas hydrophila 4ak4 were used as hosts to express tdo gene tod. plasmid pspm01, a derivative of broad-host plasmid pbbr1mcs-2 harboring tod from plasmid pkst11, was constructed and introduced into the above three strains. their abilities to catalyze the biotransformati ...200717021870
antibacterial peptides in hemocytes and hematopoietic tissue from freshwater crayfish pacifastacus leniusculus: characterization and expression pattern.a 14 amino acid residues proline/arginine-rich antibacterial peptide designated as astacidin 2 was purified and characterized from hemocytes of the freshwater crayfish, pacifastacus leniusculus. astacidin 2 has a broad range of antibacterial activity against both gram-positive and gram-negative bacteria. the primary sequence of astacidin 2 is rprpnyrprpiyrp with an amidated c-terminal and the molecular mass is 1838da determined by mass spectrometry. furthermore, the cdna of three different crust ...200717049601
rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr.buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ...200717056684
inactivation of type i polyhydroxyalkanoate synthase in aeromonas hydrophila resulted in discovery of another potential pha synthase.aeromonas hydrophila cgmcc 0911 possessing type i polyhydroxyalkanoate (pha) synthase (phac) produced only phbhhx from lauric acid but not from glucose. medium-chain-length (mcl) pha was produced from lauric acid or glucose only when phac of a. hydrophila was inactivated, indicating the existence of another pha synthase in the wild type. using pcr cloning strategy, the potential pha synthase gene (phac (mcl)) was obtained from genomic dna of the wild type and exhibited strong homology to type ii ...200717043804
characterization of twin toll-like receptors from rainbow trout (oncorhynchus mykiss): evolutionary relationship and induced expression by aeromonas salmonicida salmonicida.structure and function of factors contributing to the innate immune system of lower vertebrates, including fish are only sparsely characterized. we retrieved with rt-pcr cdna copies of two closely related toll-like receptors (tlr) from liver rna of the rainbow trout (oncorhynchus mykiss). the cdna sequences are homologous to 95.6%. the phylogenetic analysis of their deduced amino acid sequences places these twin factors closely to other known tlrs from fish. the twin factors are equally expresse ...200717070576
potential use of a transposon tn916-generated mutant of aeromonas hydrophila j-1 defective in some exoproducts as a live attenuated vaccine.aeromonas hydrophila is a gram-negative opportunistic pathogen that causes disease in a wide range of hosts due to its multifactorial virulence. here we describe the application of transposon insertion mutagenesis approach to obtain an exoenzyme mutant of a. hydrophila strain j-1. immunization of swordtail fish (xiphophorus helleri heckel) with the highly attenuated mutant provided protection (survival of 27 out of 35 fish, compared to survival of only 13 out of 35 control fish) in the fish give ...200717079040
phylogenetic identification of aeromonas strains isolated from carcasses of pig as new members of the species aeromonas allosaccharophila.the first description of the species aeromonas allosaccharophila was only based on two strains (the type strain cect4199, and a duplicate cect4200) isolated from diseased elvers (anguilla anguilla) of an eel-farm located in valencia, spain, and one stool isolate (atcc35942) from a female with diarrhoea and food poisoning in south carolina, u.s.a. in the present study, 17 aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently is ...200717080292
aeromonas hydrophila and aeromonas sobria bacteremia: rare pathogens of infection in a burn patient. 200717084982
x-ray absorption spectroscopy of the zinc-binding sites in the class b2 metallo-beta-lactamase imis from aeromonas veronii bv. sobria.x-ray absorption spectroscopy was used to investigate the metal-binding sites of imis from aeromonas veronii bv. sobria in catalytically active (1-zn), product-inhibited (1-zn plus imipenem), and inactive (2-zn) forms. the first equivalent of zinc(ii) was found to bind to the consensus zn(2) site. the reaction of 1-zn imis with imipenem leads to a product-bound species, coordinated to zn via a carboxylate group. the inhibitory binding site of imis was examined by a comparison of wild-type imis w ...200617087519
antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure.the relationship between the structure and antibacterial activity of 22 polyphenols was analyzed by using minimum inhibitory concentration (mic) as a criterion against 26 species of bacteria which can grow in mueller-hinton medium. there was no clear correlation between gram-staining and bacterial susceptibility to polyphenols, and the extent of the susceptibility was approximately dependent on the species of bacteria. in the same gram-negative bacteria, the antibacterial activity of the polyphe ...200617077519
epidemiologic cutoff values for antimicrobial agents against aeromonas salmonicida isolates determined by frequency distributions of minimal inhibitory concentration and diameter of zone of inhibition data.to develop epidemiologic cutoff values by use of frequency distributions for susceptibility to 4 antimicrobial agents when tested against a representative population of a major aquaculture pathogen, aeromonas salmonicida.200617078743
secondary aeromonas peritonitis is associated with polymicrobial ascites culture and absence of liver cirrhosis compared to primary aeromonas peritonitis.aeromonas peritonitis remains a rare condition. in this study we describe the clinical features of primary and secondary aeromonas peritonitis, and compare the differences between these two diseases entities. patients with aeromonas peritonitis were identified from microbiological and medical records during the period between march 1994 and march 2003. clinical characteristics, laboratory data, microbiological results, treatment and outcome of patients were obtained by retrospective chart review ...200617078857
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