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diarrhea associated with aeromonas hydrophila. 19863511459
classical bacterial diarrhea: perspectives and update--salmonella, shigella, escherichia coli, aeromonas and plesiomonas.impressive advancements in our understanding of the mechanisms of diarrhea production and of the epidemiologic importance of these "classical" bacterial enteropathogens have been accomplished, but many areas are in need of further exploration. the recent development of gene probes and enzyme-linked immunosorbent assays for the identification of epec, etec and eiec will enhance immeasurably the ability to carry out large scale epidemiologic studies which are still needed to clarify the global imp ...19863511463
a collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the quantum ii bid.the recently-introduced semi-automated bacterial identification system, the quantum ii bid, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. the system was evaluated independently by the two participating laboratories. inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97.8%. a further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the t ...19863512840
same day identification of gram-negative rods with the quantum ii microbiology system.the quantum ii microbiology system was challenged with 479 strains of enterobacteriaceae and 155 strains of nonfermenters (contained in the data base) and aeromonas/plesiomonas. the percentages of strains correctly diagnosed within 5h (i.e., with out additional tests) at greater than or equal to 80% probability were 78% and at greater than or equal to 50% probability, 84.7%.19863512962
bacterial diarrhoea.bacterial infections are important causes of diarrhoea in infants and children, particularly in developing countries and in other settings where standards of personal and community hygiene are low. knowledge of bacterial diarrhoeas has been significantly expanded in recent years by the finding that many episodes of acute diarrhoea are due to infections with bacteria which produce enterotoxins that interfere with intestinal fluid and electrolyte transport (the 'enterotoxigenic' diarrhoeas). sever ...19863514008
identification of enteric pathogens in the small and large intestine of children with diarrhea.enteric pathogens were identified in children with diarrhea from duodenal specimens obtained with a string capsule and from fecal specimens. rotavirus was identified in stools of 43 of 100 children, and was recovered from the small intestine from nine (21%) children who were excreting this virus. shigella was isolated from stools from 22, salmonella from 17, enterotoxigenic escherichia coli from eight, and aeromonas hydrophila from one of 100 children with diarrhea. in contrast to rotavirus, sal ...19863516549
comparison of the quantum ii bacterial identification system and the automicrobic system for the identification of gram-negative bacilli.the quantum ii bacterial identification system (bid; abbott laboratories) is a microprocessor-based spectrophotometric system for identification within 4 to 5 h of both enteric and nonenteric gram-negative bacilli. we compared the bid with the automicrobic system (ams; vitek systems, inc.), using the most recent gram-negative identification card and software (ams-gni), for the identification of 501 clinical isolates of gram-negative bacilli, including 382 belonging to the enterobacteriaceae and ...19863517034
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...19863517061
cloning, expression, and mapping of the aeromonas hydrophila aerolysin gene determinant in escherichia coli k-12.dna sequences corresponding to the aerolysin gene (aer) of aeromonas hydrophila ah2 dna were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. a plasmid containing a 5.8-kilobase ecori fragment of a. hydrophila dna was required for full expression of the hemolytic and cytotoxic phenotype in escherichia coli k-12. deletion analysis and transposon mutagenesis allowed us to localize the gene product to 1.4 kilobases of aeromonas dna and define flanking dna region ...19863522552
etiology and epidemiology of travelers' diarrhea in asia.travelers' diarrhea in asia has been studied among peace corps volunteers in thailand, japanese travelers, foreign residents in bangladesh, guests in hotels, and members of various tour groups. rates of diarrheal attack of greater than 50% during four- to six-week sojourns were reported for these groups. among travelers with diarrhea, the most commonly isolated pathogen was enterotoxigenic escherichia coli (20%-34%), followed by salmonella (11%-15%), shigella (4%-7%), campylobacter (2%-5%), and ...19863523709
multiple low-level antibiotic resistance in aeromonas salmonicida.mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ...19863524440
microbial filtrates activate granulocytes without complement or prostaglandins.cardiorespiratory dysfunction in sepsis may be mediated by circulating complement, activated leukocytes, prostaglandins, or by a direct effect of endotoxin. the purposes of this study were to determine if pathogenic microbes produce these substances and to evaluate the direct effects of substances released by micro-organisms on granulocyte aggregation (ga). escherichia coli, (e. coli), aeromonas hydrophila (aeromonas h.), staphylococcus aureus (s. aureus), and candida albicans, (candida a.) were ...19863524892
thromboxane a2 mediates hemodynamic and respiratory dysfunction in graded bacteremia.thromboxane a2 has been implicated as a mediator of cardiorespiratory dysfunction in sepsis. this study evaluated whether or not thromboxane a2 was necessary or sufficient for these adverse effects to occur during bacteremia. fourteen adult swine under barbiturate anesthesia and breathing room air were monitored with arterial and pulmonary artery catheters. animals were studied for 4 hours in three groups: group i, graded infusion of 10(9)/ml aeromonas hydrophila; group ii, aeromonas hydrophila ...19863526603
[treatment of neurosurgical bacterial meningitis using the combination of ceftriaxone-fosfomycin].16 patients with bacterial meningitis following a neurosurgical procedure were given a combination of ceftriaxone and fosfomycin. 8 microorganism were isolated: 2 staphylococcus epidermidis, 1 staphylococcus aureus, 1 neisseria meningitidis, 1 streptococcus pneumoniae, 1 haemophilus influenzae, 1 serratia marcescens and 1 aeromonas hydrophila. no pathogen was identified in the remaining cases. all of the isolated strains were susceptible to both antibiotics. in vitro, neither synergy nor antagon ...19863534726
prostacyclin and thromboxane a2 in septic shock: species differences.prostacyclin and thromboxane a2 have been implicated as mediators of septic shock. correlations between the human prostanoid response to sepsis and experimental paradigms are poorly understood. the purpose of this study was to compare changes in plasma levels of prostaglandin 6-keto-f1 alpha (pgi) and thromboxane b2 (txb) during septic shock in sprague-dawley rats, domestic pigs, mongrel dogs, and man. severe sepsis followed by septic shock (systolic bp less than 90 mmhg) was induced in rats by ...19863539388
aeromonas hydrophila peritonitis in a renal transplant recipient. 19863540131
relationship of toxin production to species in the genus aeromonas.ninety-five strains of aeromonas were divided into three species--a. sobria, a. hydrophila and a. caviae--on the basis of results in 13 biochemical tests. the minimum number of tests necessary to distinguish these species was determined. culture filtrate of the strains were tested for cytotoxin and cytotonin, haemolysin and protease. one filtrate with high-titre cytotoxin, haemolysin and protease activities was subjected to gel filtration on sephadex g75 and isoelectric focussing. of the five ce ...19863540304
intestinal infections with aeromonas. 19873544999
effect of beta-lactam prodrugs on human intestinal microflora.the ampicillin prodrugs bacampicillin, pivampicillin, and talampicillin, the mecillinam prodrug pivmecillinam and the sulbactam prodrug sulbactam pivoxil all have a greatly improved oral availability compared to the parent drug. they show no antibacterial activity themselves until transformed into active drugs after absorption. this double advantage makes them less likely to influence the intestinal microbial ecosystem. ampicillin has been reported to cause marked changes in the colon microflora ...19863547627
aeromonas salmonicida from salmonids and cyprinids--serological and cultural identification. 19863551409
research on aeromonas and plesiomonas. iv. virulence factors. 19873552716
characteristics of aeromonas species and their association with human diarrhoeal disease. 19863553304
identification of major common extracellular proteins secreted by aeromonas salmonicida strains isolated from diseased fish.ten different strains of aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree c) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. in each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight ...19873555336
epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases.an epidemiology analysis of a series of 12 aeromonas hydrophila infections, including six of septicaemia, which occurred on several wards of one hospital during the summer of 1982 is presented. the hypothesis that the hospital water could be the source of these infections was supported by the isolation of 1-10 motile aeromonads per ml in most of the water samples collected from various points on the hospital water system. electrophoretic esterase typing was used as an epidemiological screening m ...19873556437
aeromonas cytotonic enterotoxin cross reactive with cholera toxin.isolation by affinity chromatography from crude culture filtrate of aeromonas sobria of protein that cross reacted with cholera toxin (ct) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of y1 adrenal cells. all these activities were neutralised by antiserum to ct. there was no haemolytic or cytotoxic activity associated with this ct-cross reactive cytotonic enterotoxin. ct-cross reactive material detected in enzyme linked immunosorbent ...19873560188
enumeration and characterization of aeromonas hydrophila and aeromonas caviae isolated from grocery store produce.starch-ampicillin agar was used to quantitatively isolate aeromonas sp. from retail grocery store produce. all produce sampled, including parsley, spinach, celery, alfalfa sprouts, broccoli, and lettuce, contained aeromonas sp. in most instances, the count of aeromonas sp. increased 10- to 1,000-fold during 2 weeks of storage at 5 degrees c. eleven (92%) of 12 kinds of produce yielded cytotoxic aeromonas sp. identification as aeromonas hydrophila was the strongest indicator of cytotoxicity, and ...19873566266
[properties of aeromonas hydrophila enterotoxins from a food hygiene viewpoint]. 19873566431
aeromonas hydrophila: its isolation from acute diarrhoeal illness in rural bangladesh. 19863566684
growth of mixed microbial populations in ground water containing highly chlorinated organic wastes. 19873567405
[septicemia caused by aeromonas hydrophila]. 19873567701
isolation frequency of aeromonas species in relation to patient age.over a period of 18 months aeromonas spp. were isolated from stool specimens of 116 patients (total isolation frequency 1.6%). isolation rates in children up to ten years of age were significantly higher only for aeromonas caviae as compared to other age groups. additional data are needed to assess the clinical significance to these findings.19873569253
research on aeromonas and plesiomonas. ii. non-gastrointestinal diseases. 19873569483
research on aeromonas and plesiomonas. iii. enteric infections. 19873569484
diarrhoea associated with aeromonas hydrophila. 19863570383
in vitro and in vivo study of an antimicrobial activity displayed by the redmouth disease agent, yersinia ruckeri.a soluble antimicrobial product has been detected in yersinia ruckeri cultures. the contract droplets technique and the double agar layer method allowed to assess its bacteriostatic effect and the high susceptibility of other bacterial fish pathogens like vibrio and aeromonas. but it did not inhibit other strains of y ruckeri. no correlation was found between the antimicrobial activity and the virulence of the strains. as mixed infections with a salmonicida and y ruckeri could be obtained experi ...19873579181
nucleotide sequence of the gene for the hole-forming toxin aerolysin of aeromonas hydrophila.the gene for the hole-forming toxin aerolysin from aeromonas hydrophila was sequenced. although most of the sequence seems unrelated to that of staphylococcus aureus alpha-toxin, both proteins are very hydrophilic, and they each contain a nearly identical string of 10 amino acids.19873584074
congo red uptake by motile aeromonas species.virulence of several species of enteropathogenic bacteria has been correlated with the ability of isolates to take up the dye congo red. to determine whether congo red uptake might be a useful marker for virulence of motile aeromonas species, we examined 50 strains of diverse clinical origin on a medium containing 50 micrograms of congo red per ml. all of the strains took up the dye to various degrees. for most strains, uptake was greatest at 37 degrees c and least at 22 degrees c. production of ...19873584422
aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhea.in 1983, the vernacular name enteric group 77 was coined for a group of strains that had been referred to our laboratory as "possible vibrio cholerae except for gas production." by dna-dna hybridization (hydroxyapatite, 32p), 8 of 10 strains of enteric group 77 were very highly related to the labeled strain 1169-83 (74 to 100% at 60 degrees c and 75 to 100% at 75 degrees c; percent divergence, 0.0 to 2.5). type strains of six other aeromonas species were 45 to 66% related (60 degrees c) to strai ...19873584425
clinical and biochemical significance of toxin production by aeromonas hydrophila.production of cytotoxin and enterotoxin by aeromonas strains obtained from stools of 50 children in mexico and texas and from blood of 9 children with sepsis was determined. results were correlated with clinical features of infected children as well as with biochemical traits of aeromonas strains. cytotoxin was produced by 40 of 42 aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsi ...19873584426
haemagglutination patterns of aeromonas spp. related to species and source of strains.the study included 138 a. sobria and 182 a. hydrophila isolated in perth from samples of diarrhoeal or non-diarrhoeal faeces or from domestic water. strains were grouped in relation to agglutination of human, horse, rat and guinea pig erythrocytes and the effect of sugars on haemagglutination. agglutination of red cells of all four species (primary group 1) was most commonly associated with a. sobria, particularly those strains isolated from faeces of patients with diarrhoea. most a. hydrophila ...19863593124
a modification of bile salts brilliant green agar for isolation of motile aeromonas from foods and environmental specimens.aeromonas has recently been recognized as an important enteropathogen. in faecal specimens the organism is readily identified and differentiated from coliforms by the positive oxidase reaction. however, few media have proved useful for foods and environmental specimens. bile salts brilliant green starch agar (bbgs) was studied for this purpose by testing artificially contaminated foods as well as natural potential sources. the results indicate the suitability of the medium for the isolation of a ...19873595749
activities of aeromonas hydrophila hemolysins and their interaction with erythrocyte membranes.the activity of hemolysin produced by aeromonas hydrophila ca-11, isolated from an environmental source, was more sensitive to temperature than that of hemolysin produced by strain ah-1, isolated from a diarrheal case. ca-11 hemolysin failed to elicit hemolysis below 10 degrees c. immunoblotting analyses showed that both hemolysins formed into oligomers in rabbit erythrocyte membrane even when no hemolysis occurred. suggesting that the binding and the subsequent oligomerization are temperature i ...19873596803
ampicillin-dextrin agar medium for the enumeration of aeromonas species in water by membrane filtration.published selective media were evaluated for the isolation of aeromonas spp. from environmental samples by membrane filtration. satisfactory recoveries were obtained only with ma agar (rippey & cabelli) and dextrin-fuchsin-sulphite agar (schubert), but neither was sufficiently selective. the positive aspects of these two media were combined in a new medium, ampicillin-dextrin agar. recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and inc ...19873597206
hydroxamate-induced spectral perturbations of cobalt aeromonas aminopeptidase.the absorption spectrum of cobalt(ii)-substituted aeromonas aminopeptidase is markedly perturbed by the presence of equimolar concentrations of d-amino acid hydroxamates and acyl hydroxamates that have previously been shown to be powerful inhibitors of this enzyme (wilkes, s. h., and prescott, j. m. (1983) j. biol. chem. 258, 13517-13521). d-valine hydroxamate produces the most distinctive perturbation, splitting the characteristic 527 nm absorption peak of the cobalt enzyme to form peaks at 564 ...19873597389
[aeromonas hydrophila as an autochthonous causative agent of infectious enteritis in germany].aeromonas hydrophila was identified by culture technique in diarrhoeal stools and ascites in a patient of 57 years of age who suffered from known alcoholic cirrhosis and additional acute gastroenteritis. no other pathogens that would produce acute diarrhoea were found. the isolated aeromonas hydrophila strain was characterised by the pathogenic properties known to date as pertaining to this species (formation of haemolysin and enterotoxins). hence it is evident that aeromonas hydrophila occurs i ...19873608836
differentiation of motile and mesophilic aeromonas strains into species by testing for a camp-like factor.motile and mesophilic aeromonas strains can presumptively be differentiated into species in 18 to 24 h by testing the isolates for the production of a camp-like factor. aeromonas hydrophila strains were positive either aerobically or anaerobically, aeromonas sobria strains were positive only aerobically, and aeromonas caviae strains were always negative.19873611325
pyomyositis and hepatic abscess in association with aeromonas hydrophila sepsis.this report describes a patient with chronic liver disease in whom aeromonas hydrophila sepsis developed following ingestion of fresh lake water. a hepatic abscess and bilateral calf pyomyositis, an extremely rare and previously fatal complication of a. hydrophila septicemia, subsequently developed. the patient recovered following bilateral fasciotomies and systemic antibiotic therapy with gentamicin and trimethoprim/sulfamethoxazole. no previous survivors of a. hydrophila pyomyositis secondary ...19873618636
enlarging erythematous macule: a cutaneous sign for fatal aeromonas hydrophila septicemia. 19873625077
detection of fish antibody against protein antigen of aeromonas salmonicida by enzyme-linked immunosorbent assay using biotin-avidin system.antibody against aeromonas salmonicida was detected in sera from immunised or experimentally infected rainbow trout by enzyme-linked immunosorbent assay (elisa) using the biotin-avidin system. the elisa titre correlated well with the agglutinin titres of the sera, but the elisa was found to be more sensitive than the agglutination test. when the rainbow trout serum was separated by column chromatography, antibody activity (determined by elisa and agglutination test) was detected in the igm fract ...19873628989
bacteriology of the freshwater environment: implications for clinical therapy.water and animal tissue samples were obtained from sources in tennessee, california, and florida. purified bacterial colonies were isolated and organisms identified. fifty-eight isolates were recovered. twenty-seven gram-negative isolates were identified. gram-positive organisms were of the coryneform group or bacillus species. antibiotic susceptibility testing showed that aeromonas species were relatively resistant to a wide variety of antimicrobials, which included trimethoprim, cefazolin, and ...19873631666
[vibrio and aeromonad sensitivity to the vibriostatic o 129].sensitivity of vibrios and aeromonads to vibriostatic o129 (2,4-diamino-6,7-diisopropyl pteridine) was studied. the vibrios and aeromonads were isolated in the ussr. the tests were performed with disks made in the ussr and great britain. the content of the drug in the disks was 10 and 150 micrograms. it was shown that v. cholerae o1 v. cholerae not o1 and v. albensis were highly sensitive to vibriostatic o129 and produced no growth in the presence of either low or high concentrations of the drug ...19873631937
class d tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida.tetracycline resistance determinants of r plasmids from the fish pathogens aeromonas hydrophila, edwardsiella tarda, and pasteurella piscicida were classified as class d by their expression of resistance to tetracycline and minocycline and through their dna structure.19873631946
plasmids and resistance to antimicrobial agents in aeromonas sobria and aeromonas hydrophila clinical isolates.the antimicrobial susceptibilities of 75 aeromonas isolates were determined by agar dilution. differences in resistance patterns were observed between strains isolated from different geographic locations and between a. sobria and a. hydrophila isolates. multiple resistance was common; however, only one conjugative plasmid was detected. this 110-megadalton plasmid mediated resistance to eight antibiotics.19873631947
aeromonas hydrophila-associated colitis in a male homosexual.a 37-year-old homosexual man was evaluated for a one-week history of hematochezia. results of a physical examination were remarkable only for grossly bloody stool. sigmoidoscopy to 30 cm showed a friable mucosa compatible with an acute colitis, and a rectal biopsy specimen demonstrated an increased plasma cell infiltrate. stool cultures subsequently yielded aeromonas hydrophila; serum human t-cell lymphotropic virus type iii antibody titer was positive. the patient responded to a course of treat ...19873632156
phenotypic characteristics of aeromonas species isolated from adult humans.the phenotypic characteristics of 89 aeromonas strains, most of which had been isolated from feces, were examined. eighty-two percent of the isolates could be placed into one of four groups on the basis of five tests. the relationship of these groups to the three motile species of aeromonas (aeromonas caviae, a. hydrophila, and a. sobria) that have been isolated from humans is unclear. because the means for identification of aeromonas strains to the species level appear to be imprecise and becau ...19863635534
typing of aeromonas species by polyacrylamide-gel electrophoresis of radiolabelled cell proteins.a method for typing species of aeromonas by 35s-labelling and sodium-dodecyl sulphate polyacrylamide-gel electrophoresis (sds-page) of total soluble proteins is described. radiolabelled-protein profiles from different strains were compared by use of the dice coefficient. typability was 100% and discrimination of pairs of differing strains was satisfactory with acceptable reproducibility. the results also yielded information on subgroups of phenotypic species.19873656393
l-methionine gamma-lyase from pseudomonas putida and aeromonas. 19873657560
seafood shucking as an etiology for aeromonas hydrophila infection.aeromonas hydrophila has been increasingly documented as a hardy organism responsible for severe infection in the compromised host. this case report illustrates how a hydrophila may survive prolonged freezing and how seafood shucking may cause sepsis. this report serves as a caution to the immunocompromised seafood lover.19873662710
detection, quantitation and stability of the beta haemolysin of aeromonas spp.amongst 58 isolates of motile aeromonads evaluated for the ability to produce beta haemolysin, haemolytic activity was significantly associated with strains belonging to the aeromonas hydrophila and a. sobria groups. of erythrocytes from nine animal species tested, mouse red blood cells provided the best indicator system for detection of beta-haemolysin activity. furthermore, differences in the stability of the beta haemolysins of selected a. sobria and a. hydrophila isolates at different temper ...19873669057
the bacteriological quality of bottled natural mineral waters.fifty-eight bottles of natural mineral water, taken from the point of sale, were bacteriologically examined. no coliforms or aeromonas sp. were isolated from any sample. high total bacterial counts were found particularly in the still waters. most of the organisms isolated in the total counts were gram-negative rods, but gram-positive organisms were also isolated. gram-positive cocci were further identified, some of which were known human commensals suggesting contamination of the waters prior t ...19873678404
virulence markers of mesophilic aeromonads: association of the autoagglutination phenomenon with mouse pathogenicity and the presence of a peripheral cell-associated layer.autoagglutination (aa phenotype) of mesophilic aeromonads in broth was found to be a virulence-associated marker. there were two kinds of aa+ strains: those that spontaneously pelleted (sp+), and those that pelleted only after boiling (pab+). of 79 strains tested, 24 (30%) were aa+, and 18 of these were recovered from clinical specimens. most of the aa+ strains (n = 21) were identified as either aeromonas sobria or aeromonas hydrophila. of the well-documented clinical isolates of a. sobria and a ...19873679544
acute diarrhoea in adults: a prospective study.a prospective study of 74 adults with acute diarrhoea was carried out in sydney in 1984-1985 to determine the infective agents that were involved and their relationship to clinical, epidemiological and laboratory features. thirty-four potential pathogens were identified in 32 (43.2%) patients. these included, in order of frequency: campylobacter spp., rotavirus, clostridium difficile, salmonella enteritidis, aeromonas hydrophila, vibrio parahaemolyticus, adenovirus, a small round virus and giard ...19873683260
[aeromonas hydrophila in waters of lake san roque and its tributaries].the presence of aeromonas hydrophila in 72 samples of water of lake san roque and two rivers that flow into it, situated in punilla valley, córdoba was investigated. water-peptone alkaline (enrichment medium) and rippey cabelli agar without ampicillin (selective and differential medium for aeromonas hydrophila) were used for isolation. the colonies obtained were assayed by oxidase test and subsequent oxidation-fermentation of hugh leifson, motility, urease, mannitol and trehalose fermentation, o ...19863685388
virulence, cytotoxic and inflammatory activities of vibrio anguillarum and aeromonas salmonicida isolated from cultivated salmonid fish in sweden. 19873687646
comparison of selective media for primary isolation of aeromonas species from human and animal feces.five selective media were evaluated for their effectiveness in the primary isolation of aeromonas spp. from human patients with acute diarrhea and from healthy domestic animals. sheep blood agar with 30 mg of ampicillin per liter (asba30) yielded a significantly higher percentage of positive specimens as compared with the four other media. the effective combination of two selective media with which 98% of all isolates were detected and with which all of the three human-associated aeromonas spp. ...19873693536
clinical significance of aeromonas species isolated from patients with diarrhea.a total of 248 strains of aeromonas spp. were isolated from 3,334 human fecal specimens submitted to a state public health laboratory over a 2-year period to be cultured for enteric pathogens. cary-blair transport medium, blood ampicillin agar, and alkaline peptone water enrichment provided optimal recovery of aeromonas spp. a questionnaire requesting clinical and epidemiological information was sent to physicians, who submitted stool samples for testing, with each laboratory report for 107 cons ...19873693537
evaluation of quantum ii microbiology system for identification of gram-negative bacteria of veterinary origin.the ability of a rapid, semiautomated bacterial identification system, the quantum ii microbiology system (abbott laboratories, irving, tex.), to accurately identify gram-negative bacteria from veterinary sources was evaluated. a total of 378 isolates were tested, including 298 organisms in the family enterobacteriaceae and strains representing acinetobacter sp., aeromonas sp., flavobacterium sp., pasteurella multocida, plesiomonas sp., and pseudomonas spp. of these isolates, 333 (88.1%) were co ...19873693539
isolation of aeromonas sp from faecal specimens. 19873693573
aeromonas hydrophila wound infection associated with myonecrosis and gas gangrene. 19873693881
aeromonas in the gut. 19873693922
the interaction of complement components with aeromonas species.the interaction of seven serum-sensitive aeromonas strains with the complement system was investigated using a 2-h quantitative assay. of the strains tested, four isolates activated both the alternative and classical pathways, two activated only the alternative pathway, and one strain was sensitive to the bactericidal action of complement through the classical pathway only. two of the four aeromonas caviae strains were such efficient activators of the complement system that when challenged with ...19863697841
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni. 19863703054
growth of aeromonas species on enteric agars.the efficacy of eight routine enteric agars for supporting the growth of 32 strains of aeromonas spp. (17 a. hydrophila strains, 8 a. sobria strains, and 7 a. caviae strains) was investigated. the plating efficiency of aeromonas spp. on these media varied greatly (range, 0 to 100%), as did their colony size when compared with that on noninhibitory medium (5% sheep blood agar). plating efficiency on seven of these eight media appeared to be strain- and not species dependent. overall, eosin-methyl ...19863711297
production of "asao toxin" by aeromonas strains isolated from feces and drinking water.cultures of aeromonas species were tested for production of a toxin recently purified by asao et al. (t. asao, y. kinoshita, s. kozaki, t. uemura, and g. sukaguchi, infect. immun. 46:122-127, 1984) and described as a hemolysin with enterotoxic and cytotoxic activity. the toxin was produced by only 63% of aeromonas sobria strains and by 93% of aeromonas hydrophila strains. also, 54% of a. hydrophila strains produced another cytotoxic entity.19863711306
bacteria in enteric lesions of horses.thirty-three species of bacteria were isolated from the gastrointestinal mucosa of 23 adult horses and two foals. the bacteria isolated could be related to gross and microscopical lesions in some cases. clostridium perfringens type a, actinobacillus equuli, salmonella typhimurium and campylobacter coli biotype 1 could all be associated with gastrointestinal lesions. c jejuni biotype 1 and aeromonas hydrophila were both recovered in this study and have been identified as causes of enteritis in ho ...19863716108
aeromonas-related diarrhea. 19863718119
[isolation and identification of aeromonas hydrophila from diarrhea patients and its pathogenicity]. 19863719695
pathophysiology of experimental aeromonas hydrophila infection in mice.a virulent fish strain of aeromonas hydrophila was inoculated intramuscularly into laboratory mice (b10.g strain). histological, biochemical and haematological changes during the first 36 h of the infection were measured. inoculation led to septicaemia, tissue damage, endotoxic shock and death. histological examination revealed: (1) severe muscle necrosis at the injection site; (2) oedema, haemorrhage and neutrophil infiltration of the lung; and (3) focal parenchymal necrosis in the liver. signi ...19863723583
partial characterisation of a soluble haemagglutinin from human diarrhoeal isolates of aeromonas.a soluble haemagglutinin has been identified in cell-free culture supernates of human diarrhoeal isolates of aeromonas sobria, a. hydrophila and a. caviae. it was oligomeric; a major peak of haemagglutinating activity had an apparent mol. wt of 780,000 but there was haemagglutinating activity throughout the mol. wt range less than 40,000- greater than 10(6). human group o, a and b, horse, rabbit, chicken and rat erythrocytes, but not those of sheep and cow, were agglutinated by the soluble haema ...19863723584
epidemiology of aeromonas hydrophila stool isolates in a pediatric population. 19863725668
biochemical characteristics, enterotoxigenicity and susceptibility to antimicrobial agents of clinical isolates of aeromonas species encountered in the western region of saudi arabia.the biochemical characteristics, enterotoxigenicity and susceptibility to antibiotics are reported for 22 strains of aeromonas species isolated from clinical specimens in the western region of saudi arabia. aeromonas caviae was the species most frequently observed; a high proportion of these strains fermented lactose, whereas lactose fermentation was not observed in strains of a. hydrophila and a. sobria. enterotoxigenicity, as judged by cytotoxicity in tissue culture was observed in three of fo ...19863735390
quantitative and qualitative studies of gut flora in striped bass from estuarine and coastal marine environments.examination of the intestinal contents of 130 striped bass (morone saxatilis) collected from the hudson river and long island sound during may to october 1981 showed that opportunistic fish pathogens--especially aeromonas hydrophila--predominated in samples from both locations. other isolates from both groups of striped bass included vibrio, pseudomonads, flavo-bacteria, alcaligenes, and enterics. small numbers of micrococcus, bacillus, corynebacterium, and acinetobacter were also isolated. tota ...19863735580
pathogenesis of aeromonas hydrophilia induced diarrhoea. 19863741302
incidence and virulence of aeromonas species in feces of children with diarrhea.aeromonas spp. occurring in feces of children with diarrhea were studied. forty-eight strains were isolated from 2,025 specimens during a one year period. only 11 of 44 strains tested yielded virulence factors (cytotoxin, hemolysin and hemagglutinin). six strains were identified as aeromonas sobria and five as aeromonas hydrophila. the other strains isolated were identified as aeromonas caviae. the biochemical characteristics associated with virulence factors were a positive voges-proskauer reac ...19863743554
purification and characterization of an aeromonas hydrophila hemolysin.a hemolysin produced by aeromonas hydrophila ca-11, isolated from an environmental source, was purified by sulfopropyl-sephadex c-25 chromatography at ph 5.0. this hemolysin caused fluid accumulation in infant mouse intestines and rabbit intestinal loops and killed vero cells, as did the hemolysin produced by strain ah-1, isolated from a diarrheal case. in polyacrylamide gel electrophoreses at phs 4.0 and 9.4 and in thin-layer isoelectric focusing, ca-11 hemolysin migrated as a single band to a ...19863745420
isolation of aeromonas hydrophila from a case of neonatal diarrhoea (a case report). 19863746714
[aeromonas hydrophila--the causative agent of 2 episodes of acute diarrheal disease in small children]. 19863749728
the clinical significance of stool isolates of aeromonas.the purpose of this study was to determine the clinical significance of different aeromonas species isolated from stool. during a 17-month period, 29 strains of aeromonas were isolated and identified to species with the following results: 22, a. caviae, 5, a. hydrophila, and 2, a. sobria. clinical significance was determined independently of knowledge of speciation result. nineteen isolates represented colonization, implying that aeromonas can be recovered from the gastrointestinal tract without ...19863751983
potential enteropathogens in fish from lake kivu. 19863753053
clinical associations of aeromonas spp. in fecal specimens from children.human infections caused by aeromonas spp. are being reported with increased frequency. we examined 1012 unselected stool specimens from 799 children for various pathogens, including aeromonas spp., to evaluate the clinical associations of aeromonas spp. in the stools of children in the east end of london. a total of 33 children had positive cultures for aeromonas spp. these were identified as: aeromonas hydrophila (16), a. sobria (9), a. caviae (8). aeromonas spp. were identified by being oxidas ...19863757397
[aeromonas hydrophila--causative agent of severe enterocolitis?]. 19863757818
new unusual forms of colitis. report of four cases with known and unknown etiology.four patients with colitis, in two of whom the etiology was unknown, are presented. one patient with discontinuous segmental colitis was infected by aeromonas hydrophila, and another with presumed idiopathic inflammatory bowel disease was superinfected by this pathogen. although aeromonas hydrophila has been known as a rare cause of diarrhea in our region, it is in the first instance reported to cause severe long-lasting colitis as well as superinfection of idiopathic inflammatory bowel disease ...19863758910
bacterial flora associated with an organic manure-aquaculture system in kainji lake basin area, nigeria.studies on poultry-cum fish integrated aquaculture system showed contamination of water and fish by faecal and streptococci coliform, with most probable numbers of 10(5) fc/100 ml and 10(3) fc/100 ml respectively. it was also found that both water and fish muscles are contaminated with pseudomonas sp, escherichia sp., aeromonas sp. and staphylococcus sp. which are of public health significance and could play primary role as occupational disease of fish handlers.19863759357
[effect of cefminox on bacterial flora in human adult feces].cefminox (cmnx), a new cephamycin, was administered by one shot intravenous injection twice daily with a dose of 1,000 mg each time for 5 days to seven healthy male volunteers whose ages ranged from 21 to 28 years (mean: 25 years) and body weights were from 60 to 92 kg mean: 72 kg). the effect of the drug on fecal bacterial flora was investigated and the concentrations of the drug in feces were measured on 5th day before the treatment, on 0, 3rd, and 5th day (the final day of the treatment) duri ...19863761549
more on pathogenic mechanisms of aeromonas-related diarrhea. 19863763428
pronephric leucocytes of cyprinus carpio: isolation, separation and characterization.since the teleost pronephros is an important source of diverse immunocytes, suspensions of pronephric cells from young adult carp have been characterized. in freshly prepared suspensions, adherent, spreading cells (macrophages?) constituted less than 3% of the total population. granulocytes and lymphocytes were co-dominant (less than 80%) leucocyte types. continuous percoll density gradient centrifugation yielded discrete subpopulations with these rho values and cytological characteristics: frac ...19863765337
interaction between aeromonas salmonicida and peritoneal macrophages of brook trout (salvelinus fontinalis).the phagocytic and bactericidal properties of peritoneal macrophages obtained from brook trout (salvelinus fontinalis), injected with either glycogen or a modified freund's complete adjuvant (mfca), were evaluated against an avirulent and a virulent strain of aeromonas salmonicida. avirulent bacteria were effectively phagocytized and killed by macrophages obtained from fish injected with both irritants. with glycogen-elicited macrophages, no enhancement of killing was observed following opsoniza ...19863765344
effects of metals on the chemiluminescent response of rainbow trout (salmo gairdneri) phagocytes.quantification of an induced chemiluminescent (cl) response in phagocytes is currently being evaluated as an indicator system for determining those environmental pollutants that may predispose fish to disease. a cl assay was developed using phagocytes from the pronephros of rainbow trout (salmo gairdneri). the cl response of phagocytes to phorbol myristate acetate, a chemical inducer of cl, was shown to be dose-dependent. the response to five species of bacteria was also evaluated. staphylococcu ...19863765345
effect of an immunopotentiator on aeromonas salmonicida infection in rainbow trout (salmo gairdneri).the immunoactive peptide fk-565 (heptanoyl-y-d-glutamyl-(l)-mesodiaminopimelyl-(d)-alanine) was found to induce protection against intraperitoneal aeromonas salmonicida infection in rainbow trout (salmo gairdneri richardson). the survival rate was as high as 60% when fk-565 was given intraperitoneally as a single dose (1mg/kg) one day before bacterial challenge. a non-specific stimulation of phagocytic cells by fk-565 at an early stage of the bacterial infection may contribute to the resistance ...19863765348
blood changes in carp (cyprinus carpio) induced by ulcerative aeromonas salmonicida infections.only recently aeromonas salmonicida has been recognized as a significant bacterial pathogen in ulcerative disease of cyprinid fish. our attempts to formulate a vaccine based on bacterial surface antigens were unsuccessful in conferring reliable protection against lethal challenge. this lead us to study pathological changes in the humoral defense system during ulcerative a. salmonicida infection in carp. high numbers of opportunist pathogens such as a. hydrophila and pseudomonas sp. were frequent ...19863765352
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