TitleAbstractYear(sorted ascending)
gastritis in lake tanganyika cichlids (tropheus duboisii).necrotic and granulomatous gastritis is described in lake tanganyika cichlids. clostridium hastiforme and flagellated protozoa were both associated with the reaction but the significance of either is unknown. nevertheless, treatment of surviving fish with ampicillin was carried out and mortalities ceased. the possible involvement of an unsuitable diet as a predisposing factor is discussed.19853895712
a comparison of various media in the detection of aeromonas spp. from stool samples. 19853896260
role of caseinase from aeromonas salmonicida in activation of hemolysin.mutants of the bacterial fish pathogen aeromonas salmonicida selected for inability to digest casein concomitantly lost hemolytic activity against horse erythrocytes under certain conditions. mixtures of wild-type with mutant culture supernatants indicated that mutants produce an inactive precursor of a hemolysin which was activated by autogenous caseinase and, with less efficiency, by other serine proteases. selective inhibition or repression of caseinase production in the wild-type strain also ...19853897063
sch 34343: in-vitro antibacterial activity and susceptibility to beta-lactamases.the in-vitro activity of the penem sch 34343 was assessed in comparison with third-generation cephalosporins, aztreonam and imipenem. sch 34343 was active, with mics generally 2 mg/1 or less, against a broad spectrum of organisms amongst which were staphylococci (including methicillin-resistant strains), streptococci (though enterococci were less susceptible with sch 34343 mics mostly 4-8 mg/1), anaerobes (including the bacteroides fragilis group), haemophilus influenzae and neisseria gonorrhoea ...19853897169
aeromonas and plesiomonas as possible causes of diarrhoea.the number of reports on the isolation of aeromonas from patients with diarrhoeal disease is now large and suggests an etiological role of the bacterium. it is well established that strains of aeromonas produce an enterotoxin. this enterotoxin is cytotonic, i.e. it does not damage the membrane, and it does not cross-react immunologically with cholera toxin and escherichia coli heat-labile (lt) toxin. most enterotoxigenic strains also produce a cytotoxic protein (hemolysin), the role of which is ...19853899944
trat gene sequences, serum resistance and pathogenicity-related factors in clinical isolates of escherichia coli and other gram-negative bacteria.the r6-5 plasmid-specified outer membrane protein, trat protein, has previously been shown to mediate resistance to bacterial killing by serum. colony hybridization with a 700 bp dna fragment carrying most of the trat gene was used to examine the prevalence of trat in gram-negative bacteria, particularly strains of escherichia coli, isolated from clinical specimens. trat was found in isolates of e. coli, salmonella, shigella and klebsiella, but not in pseudomonas, aeromonas or plesiomonas, nor i ...19853900279
[multicenter study of the in vitro effect of imipenem (n-formimidoyl-thienamycin) on hospital bacteria].minimal inhibitory concentrations (mics) of imipenem were evaluated by agar dilution for 2 895 bacterial strains isolated in 9 hospitals. imipenem proved highly active against enterobacteriaceae, with an mic less than or equal to 0.25 for 63% of the 1 556 tested strains, less than or equal to 1 for 89.6% and less than or equal to 4 for 99%. the different groups of enterobacteriaceae exhibited similar mode mics (0.12 to 0.25), with the exception of serratia (0.25-0.5), p. mirabilis (0.5), indole- ...19853911142
thromboxane, prostacyclin, and the hemodynamic effects of graded bacteremic shock.this study investigates the interaction of thromboxane, prostacyclin, and the hemodynamic dysfunction of graded bacteremia. arterial, venous, and pulmonary artery catheters were inserted into eight adult female pigs under barbiturate anesthesia. after a 60-min control period aeromonas hydrophila (1.0 x 10(9)/ml) was infused intravenously at 0.2 ml/kg/hr, increasing gradually to 4.0 ml/kg/hr at 4 hr. hemodynamic measurements, blood gases, and radioimmunoassay of thromboxane b2 (txb) and prostagla ...19853915237
[the "water vibrio" of puntoni in the waters of castellammare di stabia]. 19853915350
[campylobacter fetus ssp. jejuni, aeromonas hydrophila, helicoidal bacteria and coronavirus in the murine intestine].intestinal contents of 28 laboratory-bred white mice and 6 wild-caught rats were extracted and observed with phase-contrast and transmission electron microscopy; cultures were made in butzler agar and aeromonas, incubated in microaerobiosis, at 37 degrees c for 5 days. in three mice and two rats, helicoidal bacteria were observed, with 8 to 11 periplasmic fibers and terminal branches of 8 to 11 structures, similar to flagella. in one of the rats, coronavirus-like particles were observed. campylo ...19853916393
resistance patterns of nonfermentative gram-negative rods and aeromonads to beta-lactam antibiotics: a diagnostic aid.consistent resistance to certain antimicrobial agents has in the past been of help in the diagnosis of nonfermentative gram-negative rods. in order to establish patterns of consistent resistance to 15 mostly newer betalactam antibiotics, 561 strains of such organisms plus aeromonas and plesiomonas were tested with the kirby-bauer method and also against the selective agent c-390 in three concentrations in mueller-hinton agar. no species-specific patterns emerged but the resistance data can be us ...19853925660
the in vitro activity and beta-lactamase stability of cefpirome (hr 810), a pyridine cephalosporin agent active against staphylococci, enterobacteriaceae and pseudomonas aeruginosa.the in vitro activity of cefpirome, a new cyclopyridinium cephalosporin, was evaluated against 947 aerobic and anaerobic bacteria. cefpirome inhibited 90% of escherichia coli, klebsiella spp., citrobacter diversus, morganella morganii, proteus vulgaris, proteus mirabilis, aeromonas spp., salmonella spp., shigella spp. and haemophilus and neisseria species at less than or equal to 0.4 mg/l. it had activity comparable to that of cefotaxime, ceftizoxime, ceftazidime, aztreonam, and moxalactam again ...19853928497
congo red agar, a differential medium for aeromonas salmonicida, detects the presence of the cell surface protein array involved in virulence.strains of the fish pathogen aeromonas salmonicida which possess the cell surface protein array known as the a-layer (a+) involved in virulence formed deep red colonies on tryptic soy agar containing 30 micrograms of congo red per ml. these were readily distinguished from colorless or light orange colonies of avirulent mutants lacking a-layer (a-). the utility of congo red agar for quantifying a+ and a- cells in the routine assessment of culture virulence was demonstrated. intact a+ cells adsorb ...19853934141
identification of hydroxy fatty acids in aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.gas-liquid chromatographic analysis of fatty acid methyl esters obtained from clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae revealed the presence of two hydroxy fatty acid species, 3-oh 12:0 and 3-oh 14:0.19853934208
in vitro activities of the quinolone antimicrobial agents a-56619 and a-56620.the in vitro activities of two new quinolone antimicrobial agents, a-56619 and a-56620, were compared with those of norfloxacin, ciprofloxacin, and other antimicrobial agents. the activity of a-56620 was comparable to that of ciprofloxacin against escherichia coli, enterobacter cloacae, and aeromonas hydrophila (mics for 90% of the strains were less than or equal to 0.06 micrograms/ml); acinetobacter anitratus and staphylococcus aureus (mic for 90% of the strains was 0.5 micrograms/ml); and most ...19853935046
[studies of aeromonas spp. isolated from patients with sporadic diarrhea]. 19853936889
phospholipids and lipopolysaccharide of aeromonas hydrophila.the phospholipids and lipopolysaccharide of aeromonas hydrophila were characterized. phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. the outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the outer membrane contained a higher proportion of saturated fatty acids. only four fatty acids (c14:0, c16:0, c16:1, and c18:1) were found in the phospholipids. the lipopolysaccharide ...19853968030
immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria.purified catabolic ornithine carbamoyltransferase of pseudomonas putida and anabolic ornithine carbamoyltransferase (argf product) of escherichia coli k-12 were used to prepare antisera. the two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by pseudomonas and representative organisms of other bacterial genera. the immunological cross-reactivity observed only between the catabolic ornithine carbamoyltrans ...19853968036
invasion of hep-2 cells by fecal isolates of aeromonas hydrophila.human diarrheal isolates of aeromonas hydrophila were found to be invasive in hep-2 cell monolayers. microscopic examination of two isolates from patients with symptoms of dysentery revealed that infected hep-2 cells contained up to 50 bacteria localized within areas of the cytoplasm. a quantitative invasion assay was developed, using viable counts of total intracellular bacteria. five fecal isolates of a. hydrophila were classified as invasive, with an average of 0.1 up to 2 bacteria per hep-2 ...19853972447
protein export by a gram-negative bacterium: production of aerolysin by aeromonas hydrophila.the synthesis and export of aerolysin, an extracellular protein toxin released by the gram-negative bacterium aeromonas hydrophila, was studied by pulse-labeling with [35s]methionine. the toxin was synthesized as a higher-molecular-weight precursor. this was processed cotranslationally, resulting in the appearance within the cell of the mature protein, which was then exported to the supernatant. precursor aerolysin accumulated in cells incubated in the presence of carbonyl cyanide m-chlorophenyl ...19853972770
pathogenicity of aeromonas.the ability of 15 aeromonas sobria and 9 aeromonas hydrophila isolates to cause subcutaneous lesions was tested. an inoculum of 10(11) colony forming units/l was injected subcutaneously into mice. surviving animals developed a subcutaneous abscess and/or localised skin sloughing and loss of hair (alopecia). an abscess was induced by all nine a. hydrophila isolates and by three of the 15 a. sobria isolates. the induction of local epidermal sloughing and loss of hair followed challenge with either ...19853981022
methods for the detection of haemagglutinins in aeromonas.when grown in specified conditions and tested by a rocked-tile method, 40 of 41 isolates of two species of aeromonas formed simultaneously at least two haemagglutinins among which were: (i) a mannose-sensitive haemagglutinins with strongest activity for guinea-pig or fowl red cells, formed by all of 31 isolates of a. hydrophila and 9 of 10 isolates of a. punctata ss. caviae; (ii) a haemagglutinin, sensitive to l-fucose or d-mannose, that reacted with human red cells and which was formed by all 4 ...19853981614
aeromonas hydrophila--an accepted enterotoxigenic "neoenteropathogen"? 19853989329
biochemical and immunological characterization of the cell surface of the fish pathogenic bacterium aeromonas salmonicida.the identification of lipopolysaccharide as periodic acid-schiff positive material, present in the membrane fraction of the fish pathogenic gram-negative bacterium aeromonas salmonicida, analyzed by sds-polyacrylamide gel electrophoresis, is shown. such analysis has revealed several periodic acid-schiff positive bands and many membrane proteins among which a pathogenicity-related mr 54000 protein as a constituent of an additional surface layer outside the outer membrane (evenberg et al., (1982) ...19853995026
humoral response and memory formation in carp after injection of aeromonas hydrophila bacterin.the humoral immune response of carp (cyprinus carpio) upon a bacterial fish pathogen aeromonas hydrophila was studied in relation to memory formation. after a single intramuscular (i.m.) injection of formalin killed a. hydrophila cells (f-ah), maximum serum ab (ab) titers were observed at day 20. distinct titers were still seen at day 360 in the groups injected with a medium or high antigen (ag) dose (10(7) respectively 10(9) f-ah). the effect of a second immunization with a high ag dose was stu ...19853996709
[spontaneous aeromonas hydrophila peritonitis in the cirrhotic. apropos of 2 cases]. 19854001643
comparative in vitro activities of selected antimicrobial agents against aeromonas species and plesiomonas shigelloides.the in vitro antimicrobial susceptibilities of fecal isolates of aeromonas caviae, aeromonas hydrophila, aeromonas sobria, and plesiomonas shigelloides were tested by agar dilution. this in vitro study suggested that ciprofloxacin, enoxacin, norfloxacin, tetracycline, and trimethoprim-sulfamethoxazole might be effective oral agents for treatment of diarrhea involving aeromonas spp. or p. shigelloides.19854004196
clinical and microbiological features of aeromonas hydrophila-associated diarrhea.the prevalence of aeromonas hydrophila in stool specimens from patients with diarrhea was studied during 18 months. a. hydrophila was found in 1.1% of patients with diarrhea and in none of 533 control patients (p less than 0.02). cases were detected 1.5 times more often during the summer months than the winter months, and most occurred in children less than 2 years of age. clinical features included fever greater than 38 degrees c (55%), abdominal cramps (35%), vomiting (25%), and duration of il ...19854008621
rapid microbiochemical method for presumptive identification of gastroenteritis-associated members of the family enterobacteriaceae.a method for rapid screening of isolates of pathogenic members of the family enterobacteriaceae is described. flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-d-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. this scheme is used to provide an inexpensive but rapid presumptive identification of salmonella, shige ...19854008622
comparison of the cathra repliscan ii, the automicrobic system gram-negative general susceptibility-plus card, and the micro-media system fox panel for dilution susceptibility testing of gram-negative bacilli.a comparative evaluation was done to test the accuracy of the cathra repliscan ii agar dilution system (diagnostic equipment, inc., st. paul, minn.), the automicrobic system with gram-negative general susceptibility-plus card (vitek systems, inc., hazelwood, mo.), and the micro-media fox panel micro broth dilution system (micro-media systems, inc., san jose, calif.) in determining mics of 12 antibiotics for 200 gram-negative bacilli. of the 200 strains tested, 12 isolates did not grow in one of ...19854008625
[gram-negative nonfermenting bacteria in respiratory tract infections]. 19854010380
isolation of aeromonas species from animals. 19854018073
invasiveness of aeromonas spp. in relation to biotype, virulence factors, and clinical features.of 69 fecal isolates of aeromonas spp., 18 had the ability to invade hep-2 cells. invasiveness correlated with biotype; of the 18 invasive strains, 16 were a. sobria and 2 were a. hydrophila. no invasive strains were found among the a. caviae. of the 18 invasive strains, 13 were enterotoxigenic. of the enterotoxigenic and invasive strains, 12 were a. sobria, but enterotoxicity was also more common among noninvasive strains of a. sobria. fucose-resistant hemagglutination was also more common in a ...19854019741
the antagonism of tetracycline and ferric iron in test the hypothesis that the in-vivo antibiotic action of tetracycline might be affected by ferric iron and the enhancement of infection by ferric iron by tetracycline, the actions of intraperitoneal antibiotic and local ferric ammonium citrate, given separately and together, were measured in the dorsal skin of guinea-pigs bearing lesions due to staphylococci, streptococci, a proteus sp., an erysipelothrix sp., clostridium perfringens, pseudomonas aeruginosa, aeromonas hydrophila and klebsiel ...19854020849
bacteremia caused by aeromonas species in hospitalized cancer patients.bacteremia caused by aeromonas species occurred in 24 hospitalized patients in a cancer institute during a 13-year period. all but one of these patients had a malignancy (88% had leukemia), and most were receiving chemotherapy for cancer. there was a striking numerical predominance of male patients (82%). unlike some previously described patients with infections due to this organism, none of these 24 patients had recently been exposed to fresh or salt water or to fish. the source of the infectin ...19854023526
a transition-state-analog inhibitor influences zinc-binding by aeromonas aminopeptidase.the transition-state-analog inhibitor, 1-butaneboronic acid, markedly enhances the uptake of one g-atom of zn2+ ions from a metal ion buffer system by zn-depleted aeromonas aminopeptidase. in contrast, a substrate-analog inhibitor, n-valeramide, does not perturb the equilibrium between zn2+ ions and the enzyme in a metal ion buffer system. these results establish a role for metal ions in the binding of 1-butaneboronic acid to aeromonas amino-peptidase and strongly imply that a bound zn2+ ion int ...19854026862
salmonellae and pollution indicator bacteria in municipal and food processing effluents and the cornwallis river.a study was conducted to determine the incidence of fecal coliforms, fecal streptococci, aeromonas hydrophilia, and salmonella spp. in the waste discharges of seven sewage treatment plants, four fruit and vegetable canneries, a meat packing plant, a poultry processing plant, and a potato processing plant located along the cornwallis river in nova scotia, canada. surface water samples were also collected from 13 locations in the river to assess the impact of these waste discharges on the receivin ...19854027779
bacteria in bivalve shellfish with special reference to the oyster.the bacterial flora of the pacific oyster crassostrea gigas, the sea mussel perna viridis and the arkshell clam scapharca cornea differed considerably from that of seawater in both numbers and generic composition. the numbers of heterotrophic bacteria in the bivalve shellfish, including the anaerobes and spore-forming bacteria, were greater than that in the surrounding water. pseudomonas spp. were the dominant organisms, comprising over one third of the 321 strains characterized after isolation ...19854030530
[amino acid composition of white poultry meat contaminated with psychrophilic microorganisms].studied was the effect of psychrophilic organisms of the acinetobacter, aeromonas, alcaligenes, and flavobacter genera on the amino acid composition of white poultry meat stored at 0 degrees to 4 degrees c in the course of 48 hours, and at 18 degrees c in the course of 6 to 12 months. the quantitative determination of individual amino acids was carried out by means of an automatic amino acid analyzer, hitachi type, after the method of speckman, stein, and moore. no changes were established in th ...19854035999
in vitro susceptibilities of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae to 22 antimicrobial agents.mics of 22 antimicrobial agents for 60 strains of three aeromonas species were determined by a microdilution method. the newer cephalosporins such as moxalactam, cefotaxime, and cefoperazone, the aminoglycosides, and chloramphenicol, tetracycline, nitrofurantoin, and trimethoprim-sulfamethoxazole inhibited most of the strains studied. within the genus, a. hydrophila was more resistant than either a. caviae or a. sobria to the antibiotics tested.19854037775
immunity to aeromonas salmonicida in coho salmon (oncorhynchus kisutch) induced by modified freund's complete adjuvant: its non-specific nature and the probable role of macrophages in the phenomenon.juvenile coho salmon (oncorhynchus kisutch), vaccinated with one intraperitoneal injection of formalin-killed virulent aeromonas salmonicida cells suspended in saline, showed increased protection against approximately one ld60 of homologous challenge administered at 30 days post-vaccination. under similar conditions, coho vaccinated with a modified complete freund's adjuvant (mfca) alone were also equally protected. when measured against a more severe a. salmonicida challenge of approximately on ...19854043481
demonstration of cholera toxin-related factor in cultures of aeromonas species by enzyme-linked immunosorbent assay.the production of toxins by aeromonas species was examined by the suckling mouse test, the hemolysin test, and the enzyme-linked immunosorbent assay with anticholera enterotoxin. a factor that was immunologically related to cholera enterotoxin was produced by 5 of 14 strains of aeromonas hydrophila and 4 of 15 strains of aeromonas sobria. analysis by these assays and by a test for heat stability suggested that the factor differed from hemolysin and from toxin that was active in the suckling mous ...19854044041
lack of correlation between known virulence properties of aeromonas hydrophila and enteropathogenicity for humans.five strains of aeromonas hydrophila were selected for use in volunteer challenge trials. all five strains produced cytotoxin, hemolysin enterotoxin, lysine decarboxylase, acetylmethylcarbinol, and dnase. two strains hydrolyzed esculin. all strains produced purulent hemorrhagic fluid accumulation in rabbit ileal loops, but failed to induce keratoconjunctivitis in guinea pigs. none of the strains produced mannose-resistant hemagglutinins. in challenge studies, diarrhea was demonstrated in only 2 ...19854044042
the purification and some properties of h-lysin from aeromonas salmonicida.h-lysin from aeromonas salmonicida has been purified 1770-fold by freeze fractionation, ammonium sulphate precipitation, ion exchange chromatography and gel filtration chromatography. the purified material was predominantly h-lysin, devoid of detectable t-lysin, caseinase or gelatinase activity, although glycerophospholipid: cholesterol acyltransferase (gcat) activity was present. the results suggested that h-lysin and gcat activities were due to different extracellular products. studies of the ...19854045422
clinical predictors of acute bacterial diarrhea in young children.this prospective study assessed the value of presenting history, physical examination, and screening laboratory tests in predicting whether diarrhea in a young child is associated with a stool culture positive for a bacterial pathogen. acutely ill children less than 4 years old were studied in a hospital outpatient setting. two hundred patients were seen in a 9 1/2-month period, which encompassed the seasons of summer, fall, and winter. one hundred ninety-five patients had cultures completed and ...19854047797
[metabolic traits of plesiomonas shigelloides strains isolated from children with acute enteritis]. 19854048753
unfamiliar bacteria isolated from cats. 19854049692
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
cephalothin susceptibility as a potential marker for the aeromonas sobria group.fifty-four motile aeromonas strains, composing the three currently recognizable species, were tested for susceptibility to cephalothin by broth dilution and disk agar diffusion assays. cephalothin susceptibility was significantly associated with aeromonas sobria (p less than 0.001) and may be an additional phenotypic marker useful in the identification of this species.19854056009
media for the isolation of aeromonas hydrophila.isolation rates of aeromonas hydrophila from stool samples of symptomatic and asymptomatic individuals were examined for several common enteric media. sheep blood agar with 10 micrograms of ampicillin per ml, preceded by overnight enrichment in alkaline peptone water, yielded 2.6 times the number of isolates as the other media examined and is recommended for the isolation of a. hydrophila from humans.19854056015
recurrent aeromonas hydrophila septicemia, a case report. 19854056647
[aeromonas hydrophila bacteremia. clinical and bacteriological characteristics. apropos of 16 cases]. 19854059610
antibacterial activity of amifloxacin (win 49, 375), a new quinolone agent.the in vitro activity of amifloxacin, a quinolone antimicrobial agent was compared with those of ciprofloxacin, enoxacin, ofloxacin and norfloxacin against gram-positive and gram-negative bacteria. ninety percent of escherichia coli, klebsiella species, aeromonas, salmonella, shigella, citrobacter, enterobacter species, proteus mirabilis, serratia marcescens, and morganella morganii were inhibited by less than or equal to 0.5 microgram/ml. amifloxacin inhibited branhamella, haemophilus, and neis ...19854064609
aeromonas caviae: an enteric pathogen?of the aeromonas spp. isolated routinely in our laboratory from human feces, about two thirds represent aeromonas caviae. in contrast to aeromonas hydrophila and aeromonas sobria, this species has been considered to be of little enteropathogenic significance due to the absence of known virulence factors. the clinical data presented here suggest a clinical significance of a. caviae, at least in some cases. as yet nothing is known about the pathogenic mechanisms involved.19854066046
peritrichous flagella in mesophilic strains of aeromonas.a total of 186 mesophilic strains of aeromonas, comprising 151 a. hydrophila and 35 a. caviae, were tested for peritrichous flagella by leifson's staining method. when incubated on solid medium for 18 hr at 22 c, peritrichous flagella were demonstrated in 28 (18.5%) of the 151 strains of a. hydrophila and in 6 (17.1%) of the 35 strains of a. caviae. the peritrichous flagella in these 34 mesophilic strains of aeromonas were also observed by electron microscopy.19854068347
ticarcillin plus clavulanic acid versus moxalactam therapy of osteomyelitis, septic arthritis, and skin and soft tissue infections.a controlled, randomized study to compare the efficacy and safety of ticarcillin plus clavulanic acid with moxalactam was carried out in 25 evaluable patients with bone, joint, and skin or skin structure infections. of the 13 patients in the ticarcillin plus clavulanic acid-treated group, nine had osteomyelitis, two had septic arthritis, one had cellulitis, and one had a wound infection. four of the 12 moxalactam-treated patients had osteomyelitis, one had septic arthritis, and the other seven h ...19854073082
aeromonas-related diarrhea in adults.we have reviewed the incidence of aeromonas in patients with enteric disease at our hospital and found it to be the highest of any potential enteric pathogen. eighty adult patients with diarrhea had aeromonas isolated from feces, and in 73 aeromonas was the only potential bacterial or parasitic pathogen detected. the spectrum of illness in patients with aeromonas-related diarrhea ranged from acute, self-limited diarrhea to a chronic, indolent diarrheal illness. sixteen percent (13/80) of the pat ...19854074035
prevalence and pathogenicity of aeromonas hydrophila.a field survey to determine the prevalence of aeromonas hydrophila revealed a recovery rate of 8% in 141 specimens derived from a range of live, companion, and exotic avian species. the prevalence rate was similar in 240 sequential postmortem submissions during 1984. studies on the pathogenicity of a. hydrophila showed that 2-to-4-day-old chicks and turkey poults were highly susceptible to exposure via the subcutaneous, yolk-sac, and intracerebral routes, and mortality of 80-100% occurred within ...19854074237
spectral and kinetic studies of metal-substituted aeromonas aminopeptidase: nonidentical, interacting metal-binding sites.apoenzyme prepared by removal of the 2 mol of zn2+/mol from aeromonas aminopeptidase is inactive. addition of zn2+ reactivates it completely, and reconstitution with co2+, ni2+, or cu2+ results in a 5.0-, 9.8-, and 10-fold more active enzyme than native aminopeptidase, respectively. equilibrium dialysis and spectral titration experiments with co2+ confirm the stoichiometry of 2 mol of metal/mol. the addition of only 1 mol of metal/mol completely restores activity characteristic of the particular ...19854074699
antigen localization in the lymphoid organs of carp (cyprinus carpio).a brief morphological description is given of the spleen, head kidney and trunk kidney, the three peripheral lymphoid organs of carp. an antigen-localization study was carried out using aeromonas hydrophila (a cellular bacterial antigen) and an indirect immunofluorescence test. examination of the lymphoid organs at various times after injection (up to 12 months) showed that antigen was at first present in splenic ellipsoids and in solitary phagocytic cells in the spleen, head and trunk kidney. l ...19854075373
an unusual epizootic of anuran aeromoniasis. 19854077645
growth of aeromonas spp. on cefsulodin-irgasan-novobiocin agar selective for yersinia enterocolitica.twenty-eight strains of aeromonas spp. were analyzed for their ability to grow on two different kinds of cefsulodin-irgasan (triclosan; ciba-geigy ag, basel, switzerland)-novobiocin (cin) agar containing 15 or 4 mg of cefsulodin per ml and on inositol-bile salts-brilliant green (ibb) agar. relative to blood agar, 68% of the strains were inhibited by more than 2 logs (i.e., less than 1% growth) at 37 degrees c (39% at 25 degrees c) on cin i (high cefsulodin concentration), 7% were inhibited at ei ...19854077959
holding effects on coliform enumeration in drinking water samples.standard procedures for analyzing drinking water stress the need to adhere to the time and temperature conditions recommended for holding samples collected for microbiological testing. the national drinking water laboratory certification program requires compliance with these holding limits, but some investigators have reported difficulties in meeting them. research was conducted by standard analytical methods to determine if changes occurred when samples were held at 5 and 22 degrees c and anal ...19854083877
[isolation of aeromonas hydrophila in diarrhea. characterization of enterotoxinogenic strains and clinical relations].aeromonas hydrophila is isolated from diarrhoea specimens with increasing frequency. the interest in this organism at the present time is related to the fact that it can produce a number of toxins, in particular alpha and beta cytotoxic haemolysins, an enterotoxin and various enzymes. the authors determined the frequency of isolation of this organism and tested the haemolytic, cytotoxic and enterotoxic effects of culture filtrates in all of the stool specimens received in their laboratory over a ...19854091318
the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens.the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ...19852941257
starch-ampicillin agar for the quantitative detection of aeromonas hydrophila.interest in aeromonas hydrophila as a food-borne and human pathogen is increasing. isolation media from the clinical laboratory were evaluated for food use and either did not give quantitative recovery of a. hydrophila or did not permit ready differentiation of a. hydrophila from the background microflora. a new medium was developed which permitted quantitative recovery of a. hydrophila from foods. the medium consisted of phenol red agar base (difco laboratories), soluble starch (10 g/liter), an ...198516346899
significance of extracellular protease for growth of a heterotrophic bacterium, aeromonas salmonicida.the role of protease produced by a heterotrophic bacterium during growth was investigated with aeromonas salmonicida, the pathogen of fish furunculosis, strain a-7301 and its protease-deficient mutant ntg-1 induced by mutagenesis. strain a-7301 produced extracellular protease in a mixed amino acid medium (composed of gly, ala, val, ile, leu, thr, ser, cys, met, phe, tyr, lys, arg, pro, his, try, asp, asn, glu, and gln at equal concentrations of 0.1 g/liter). its multiplication rate was limited b ...198516346900
temporal occurrence of vibrio species and aeromonas hydrophila in estuarine sediments.marine sediments were assayed for their concentration of vibrio spp. and aeromonas hydrophila over 1 year. a temporal variation was observed in which a. hydrophila, and to a lesser degree v. fluvialis, were found in the winter months, v. parahaemolyticus and v. vulnificus predominated in the spring and summer, with non-o-1 v. cholerae and v. alginolyticus detected in the late summer and fall. these organisms were found in greatest numbers in the top 5 cm of sediment, but were detectable down to ...198516346948
evaluation of different assay systems for identification of environmental aeromonas strains.important biochemical reactions in conventional tests were compared with counterpart reactions in two multiple test systems, api-20e (analytab products, plainview, n.y.) and aeromonas hydrophila medium, to evaluate their accuracy for the identification of motile aeromonas spp. isolated from fish. in a total of 49 aeromonas spp. isolates and 10 a. hydrophila reference strains, false-negative or -positive reactions were detected in the voges-proskauer test, indole production, gelatinase activity, ...198616347025
evaluation of different assay systems for identification of environmental aeromonas strains.[this corrects the article on p. 655 in vol. 51.].198616347094
electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water.[this corrects the article on p. 1343 in vol. 51.].198616347195
electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water.the ecological mechanism of survival of aeromonas salmonicida, the bacterial pathogen of fish furunculosis, in river water was investigated by laboratory-based experiments with two virulent strains (which were autoagglutinating) and two virulent strains (which were nonagglutinating). a difference in net electrical charge of a. salmonicida cells was detected by electrophoresis; cells of the virulent strains were negative, whereas cells of the avirulent strains were positive. despite the loss of v ...19862942106
applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.). 19862953200
the comparative in-vitro activity of pefloxacin.the in-vitro antibacterial activities of pefloxacin, other 4-quinolones and representative beta-lactams and aminoglycosides were assessed by determination of minimum inhibitory concentrations (mics). pefloxacin (mics mostly 0.03-2 mg/l) was highly active against enterobacteriaceae. gentamicin had slightly lower activity, and ceftazidime and norfloxacin similar activities to pefloxacin whereas ciprofloxacin was more active. pefloxacin (mics 0.03-2 mg/l) was active against acinetobacter but again ...19862940213
in-vitro activity of newer quinolones against aerobic bacteria.nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ...19862940214
antimicrobials and fish: a review of drugs used to treat bacterial diseases of channel catfish and rainbow trout.the principal bacterial diseases of cultured rainbow trout (salmo gairdneri) and channel catfish (ictalurus punctatus), and the antibacterials used in their treatment are briefly reviewed. gram-negative bacteria predominate, and oxytetracycline is effective in treatment of infections induced by them. the only antibacterials now registered by the us food and drug administration are sulfamerazine for the treatment of furunculosis in trout, and oxytetracycline for the treatment of furunculosis in s ...19863334692
aeromonas infection following facial injury. 19863459840
bacteremia due to aeromonas hydrophila: a report of 40 episodes. 19863461126
activity of newer antimicrobial agents against aeromonas hydrophila. 19863463508
the in-vitro activity of ci-934 compared with that of other new 4-quinolones and nalidixic acid.the in-vitro activity of ci-934, a new 4-quinolone compound, was compared with that of the other new 4-quinolones, enoxacin and ciprofloxacin, and also with that of nalidixic acid. ci-934 was more active than any of the other 4-quinolones tested against gram-positive aerobic organisms including staphylococcus aureus (mics 0.06-0.25 mg/l), beta-haemolytic streptococci (mics 0.12-0.5 mg/l), streptococcus pneumoniae (mics 0.25-0.5 ml/l), viridans streptococci (mics 0.06-0.5 mg/l) and most enterococ ...19863463557
the comparative in-vitro activity of eight newer quinolones and nalidixic acid.the in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, a-56619, a-56620 and ci-934 was assessed by determination of mics. the 4-quinolones were all highly active against most isolates of enterobacteriaceae, including nalidixic acid-resistant strains. ciprofloxacin (mics 0.002-2 mg/l) was the most active and a-56619 (mics 0.008-32 mg/l) was the least active. a-56619, a-56620, ofloxacin, ciprofloxacin and ci-934 were ...19863468100
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
stress factors that can affect studies of drug metabolism in fish.the effects of environmental stress conditions on the defense response of rainbow trout following a four week exposure to subacute levels of un-ionized ammonia or temperatures 5 c above and 5 c below the temperature optimum (15 c) were investigated. these experimental studies can serve as a model to evaluate the metabolic response of fish to external agents (e.g., drugs, vaccines) under environmental conditions seen in the culture of fish. blood and tissue immune parameters measured include hema ...19863509652
diarrhea associated with aeromonas hydrophila. 19863511459
classical bacterial diarrhea: perspectives and update--salmonella, shigella, escherichia coli, aeromonas and plesiomonas.impressive advancements in our understanding of the mechanisms of diarrhea production and of the epidemiologic importance of these "classical" bacterial enteropathogens have been accomplished, but many areas are in need of further exploration. the recent development of gene probes and enzyme-linked immunosorbent assays for the identification of epec, etec and eiec will enhance immeasurably the ability to carry out large scale epidemiologic studies which are still needed to clarify the global imp ...19863511463
a collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the quantum ii bid.the recently-introduced semi-automated bacterial identification system, the quantum ii bid, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. the system was evaluated independently by the two participating laboratories. inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97.8%. a further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the t ...19863512840
same day identification of gram-negative rods with the quantum ii microbiology system.the quantum ii microbiology system was challenged with 479 strains of enterobacteriaceae and 155 strains of nonfermenters (contained in the data base) and aeromonas/plesiomonas. the percentages of strains correctly diagnosed within 5h (i.e., with out additional tests) at greater than or equal to 80% probability were 78% and at greater than or equal to 50% probability, 84.7%.19863512962
bacterial diarrhoea.bacterial infections are important causes of diarrhoea in infants and children, particularly in developing countries and in other settings where standards of personal and community hygiene are low. knowledge of bacterial diarrhoeas has been significantly expanded in recent years by the finding that many episodes of acute diarrhoea are due to infections with bacteria which produce enterotoxins that interfere with intestinal fluid and electrolyte transport (the 'enterotoxigenic' diarrhoeas). sever ...19863514008
identification of enteric pathogens in the small and large intestine of children with diarrhea.enteric pathogens were identified in children with diarrhea from duodenal specimens obtained with a string capsule and from fecal specimens. rotavirus was identified in stools of 43 of 100 children, and was recovered from the small intestine from nine (21%) children who were excreting this virus. shigella was isolated from stools from 22, salmonella from 17, enterotoxigenic escherichia coli from eight, and aeromonas hydrophila from one of 100 children with diarrhea. in contrast to rotavirus, sal ...19863516549
comparison of the quantum ii bacterial identification system and the automicrobic system for the identification of gram-negative bacilli.the quantum ii bacterial identification system (bid; abbott laboratories) is a microprocessor-based spectrophotometric system for identification within 4 to 5 h of both enteric and nonenteric gram-negative bacilli. we compared the bid with the automicrobic system (ams; vitek systems, inc.), using the most recent gram-negative identification card and software (ams-gni), for the identification of 501 clinical isolates of gram-negative bacilli, including 382 belonging to the enterobacteriaceae and ...19863517034
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...19863517061
cloning, expression, and mapping of the aeromonas hydrophila aerolysin gene determinant in escherichia coli k-12.dna sequences corresponding to the aerolysin gene (aer) of aeromonas hydrophila ah2 dna were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. a plasmid containing a 5.8-kilobase ecori fragment of a. hydrophila dna was required for full expression of the hemolytic and cytotoxic phenotype in escherichia coli k-12. deletion analysis and transposon mutagenesis allowed us to localize the gene product to 1.4 kilobases of aeromonas dna and define flanking dna region ...19863522552
etiology and epidemiology of travelers' diarrhea in' diarrhea in asia has been studied among peace corps volunteers in thailand, japanese travelers, foreign residents in bangladesh, guests in hotels, and members of various tour groups. rates of diarrheal attack of greater than 50% during four- to six-week sojourns were reported for these groups. among travelers with diarrhea, the most commonly isolated pathogen was enterotoxigenic escherichia coli (20%-34%), followed by salmonella (11%-15%), shigella (4%-7%), campylobacter (2%-5%), and ...19863523709
multiple low-level antibiotic resistance in aeromonas salmonicida.mutants with multiple low-level antibiotic resistance were isolated from virulent wild-type aeromonas salmonicida strains exposed to a low concentration of any one of several low-molecular-mass (approximately 635 daltons or less) antibiotics. multiple resistance was toward beta-lactam compounds (penicillin g, ampicillin, cloxacillin), quinolones (flumequine, oxolinic acid, nalidixic acid), tetracyclines, chloramphenicol, and novobiocin. susceptibilities of the mutants toward several higher-molec ...19863524440
microbial filtrates activate granulocytes without complement or prostaglandins.cardiorespiratory dysfunction in sepsis may be mediated by circulating complement, activated leukocytes, prostaglandins, or by a direct effect of endotoxin. the purposes of this study were to determine if pathogenic microbes produce these substances and to evaluate the direct effects of substances released by micro-organisms on granulocyte aggregation (ga). escherichia coli, (e. coli), aeromonas hydrophila (aeromonas h.), staphylococcus aureus (s. aureus), and candida albicans, (candida a.) were ...19863524892
thromboxane a2 mediates hemodynamic and respiratory dysfunction in graded bacteremia.thromboxane a2 has been implicated as a mediator of cardiorespiratory dysfunction in sepsis. this study evaluated whether or not thromboxane a2 was necessary or sufficient for these adverse effects to occur during bacteremia. fourteen adult swine under barbiturate anesthesia and breathing room air were monitored with arterial and pulmonary artery catheters. animals were studied for 4 hours in three groups: group i, graded infusion of 10(9)/ml aeromonas hydrophila; group ii, aeromonas hydrophila ...19863526603
[treatment of neurosurgical bacterial meningitis using the combination of ceftriaxone-fosfomycin].16 patients with bacterial meningitis following a neurosurgical procedure were given a combination of ceftriaxone and fosfomycin. 8 microorganism were isolated: 2 staphylococcus epidermidis, 1 staphylococcus aureus, 1 neisseria meningitidis, 1 streptococcus pneumoniae, 1 haemophilus influenzae, 1 serratia marcescens and 1 aeromonas hydrophila. no pathogen was identified in the remaining cases. all of the isolated strains were susceptible to both antibiotics. in vitro, neither synergy nor antagon ...19863534726
prostacyclin and thromboxane a2 in septic shock: species differences.prostacyclin and thromboxane a2 have been implicated as mediators of septic shock. correlations between the human prostanoid response to sepsis and experimental paradigms are poorly understood. the purpose of this study was to compare changes in plasma levels of prostaglandin 6-keto-f1 alpha (pgi) and thromboxane b2 (txb) during septic shock in sprague-dawley rats, domestic pigs, mongrel dogs, and man. severe sepsis followed by septic shock (systolic bp less than 90 mmhg) was induced in rats by ...19863539388
aeromonas hydrophila peritonitis in a renal transplant recipient. 19863540131
relationship of toxin production to species in the genus aeromonas.ninety-five strains of aeromonas were divided into three species--a. sobria, a. hydrophila and a. caviae--on the basis of results in 13 biochemical tests. the minimum number of tests necessary to distinguish these species was determined. culture filtrate of the strains were tested for cytotoxin and cytotonin, haemolysin and protease. one filtrate with high-titre cytotoxin, haemolysin and protease activities was subjected to gel filtration on sephadex g75 and isoelectric focussing. of the five ce ...19863540304
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