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the nucleotide sequence of the argt locus of aeromonas hydrophila. 19883205735
serological comparison between isolated strains of aeromonas salmonicida by agglutination and enzyme-linked immunosorbent assay (elisa). 19883206945
antibiotic sensitivities of aeromonas hydrophila cultured from medicinal leeches.increased use of medicinal leeches (hirudo medicinalis) for the treatment of venous congestion in flaps and replanted parts has coincided with reports of soft tissue infections following leech application. we cultured the gullets of 20 medicinal leeches to re-examine the antibiotic sensitivities of aeromonas hydrophila, the leech enteric organism associated with reported infections. these isolates reflected reported resistance to penicillin and first generation cephalosporins as well as sensitiv ...19883207967
[bacteria reducing chromium in nature and in effluents of industrial plants].bacteria capable of hexavalent chromium (cr6+) reduction can be found in cr6+-containing sewage and sediments of purification tanks of industrial plants. they cannot be detected in water and soil samples containing no chromium compounds. bacteria reducing chromium belong to the genera aeromonas, escherichia, pseudomonas and enterobacter. their activity of cr6+ reduction correlates with the high resistance to the elevated content of this ion in the medium. the fine cell structure of these bacteri ...19883211011
evaluation of several selective media for recovery of aeromonas hydrophila from polluted waters.eleven media were studied for their suitability in the selective isolation of aeromonas hydrophila. preliminary results showed that five of them (inositol-brilliant green-bile salts agar, bile salts-brilliant green agar, rimler-shotts agar, xylose-sodium deoxycholate-citrate agar, and dextrin-fuchsin-sulfite agar) allowed the growth of several microorganisms that are usually present in the same samples in which a. hydrophila is found. six media (ma agar, modified rimler-shotts agar, dnase-toluid ...19883214158
nutritional versatility and growth kinetics of an aeromonas hydrophila strain isolated from drinking water.the nutritional versatility and growth kinetics of aeromonas hydrophila were studied to determine the nature and the growth-promoting properties of organic compounds which may serve as substrates for the growth of this organism in drinking water during treatment and distribution. as an initial screening, a total of 69 different organic compounds were tested at a concentration of 2.5 g/liter as growth substrates for 10 a. hydrophila strains. of these strains, strain m800 attained the highest maxi ...19883214162
enteropathogenicity of aeromonas species isolated from infants: a cohort study.the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ...19883216131
[toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883216765
[immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae]. 19883216766
[which are the most frequently occurring enteropathogenic bacteria in clinical practice?].diarrhea is an increasingly frequent clinical finding in swiss medical practice. one reason is the volume of tourism in countries with different climatic and social circumstances. to obtain an overview of the enteropathogenic bacteria spectrum as a cause of diarrhea, we analyzed 11,163 stool specimens from a representative group of patients throughout switzerland. enteropathogenic bacteria were found in 15.2% of all specimens; 50% were campylobacter jejuni/coli, 35% salmonella spp., 8% aeromonas ...19883217775
carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod.the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ...19883218392
[the occurrence of bacterial infections in the swamp beaver (myocaster coypus molina, 1782). 3. streptococcal, staphylococcal, aeromonas and actinobacillus infections]. 19883219032
in vitro activity of cefixime, a new oral cephalosporin.the in vitro activities of cefixime and seven comparative oral antimicrobials were studied. mic90s of cefixime were 0.015-1 micrograms/ml for haemophilus influenzae, branhamella catarrhalis, escherichia coli, klebsiella, citrobacter diversus, proteus, providencia, aeromonas hydrophila and streptococcus. mic90s for other enterobacteriaceae were greater than or equal to 2 micrograms/ml; 44% of those strains were inhibited by less than or equal 1 microgram/ml. staphylococcus, psuedomonas aeruginosa ...19883219746
seasonal distribution of aeromonas hydrophila in river water and isolation from river fish.the seasonal distribution of aeromonas hydrophila in water and recovery rate from live river fish was investigated. the highest isolation rates of a. hydrophila occurred in water during the late winter followed by a progressive decline in density during the summer and monsoon seasons. the organism was recovered from fish throughout the period from which it was concluded that they form a reservoir which is unrelated to their density in water. the enterotoxigenicity of some environmental strains w ...19883220801
clinical safety and efficacy of ofloxacin.in this study we report about the efficacy and tolerability of ofloxacin in the treatment of 15 patients with severe and moderately severe infections including osteomyelitis (5), soft tissue infections (5), salmonellosis in aids patients (2), acute or chronic pulmonary infections (2) and mediastinitis (1). the following organisms were isolated in culture specimens: staphylococcus aureus (4), pseudomonas aeruginosa (4), staphylococcus epidermidis (3), serratia marcescens (1), escherichia coli (1) ...19883224401
aeromonas infection following a snake bite: a case report.a twelve year-old thai boy with fulminating aeromonas hydrophila infection of the right arm following a snake bite was reported. he recovered uneventfully after appropriate antimicrobial therapy and surgical intervention.19883227407
correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity.certain strains of mesophilic aeromonads (aeromonas hydrophila, a. sorbria, and a. caviae), when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. because these strains are nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as a means of understanding animal virulence and enteropathogenicity. to assess virulence, batches of five white mice were inoculated intraperitoneally with 10(7 ...19883230136
preparation of hemolysin from aeromonas. 19883231101
electrophoretic data in designing strategies for purification and identification of a highly polymorphic bacterial esterase.we have purified a bacterial enzyme, designated esterase m, by tailoring an efficient and rapid strategy with information derived from titration curves of proteins in crude extract. the ph-dependent stability of the enzyme activity observed by titration pattern allowed an acidic ph treatment of extract and a cationic exchange chromatography at ph 4.1. these two steps were followed by an anionic exchange chromatography and a preparative electrophoresis. thus, the enzyme was purified about 2000-fo ...19883235767
[septicemia caused by aeromonas/plesiomonas in adults]. 19883244913
[serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae]. 19883246916
[characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883246917
[an epidemic of food poisoning by aeromonas hydrophila]. 19883248456
three-dimensional structure of the crystalline surface layer from aeromonas hydrophila.the three-dimensional (3d) structure of the crystalline surface layer of aeromonas hydrophila has been determined to a resolution of 1.3 nm by crystallographic electron microscopy. the s-layer has tetragonal symmetry, with a unit cell dimension of 12.0 nm and a thickness of 5.6 nm. the 3d reconstruction reveals a distinct domain structure, with one main protein mass at one of the fourfold symmetry axes and a minor part at the other.19883249041
aeromonas hydrophila septicaemia and muscle abscesses associated with immunosuppression.we report a case of septicaemia and muscle abscesses due to aeromonas hydrophila. the patient was immunosuppressed due to hodgkin's disease and treatment with cytotoxic chemotherapy. the abscesses resolved after prolonged antibiotic therapy. organisms that were once considered rarely pathogenic are emerging as increasingly important in causing potentially lethal infections in immunosuppressed patients.19883251226
aeromonas hydrophila cellulitis and wound infections caused by waterborne organisms. 19883257483
beta-lactam resistance in aeromonas spp. caused by inducible beta-lactamases active against penicillins, cephalosporins, and carbapenems.use of cefoperazone in a patient with aeromonas caviae in the respiratory tract selected a mutant that constitutively produced beta-lactamase. this mutant, in contrast to its parental strain with an inducible beta-lactamase, showed enhanced resistance to newer cephalosporins and aztreonam. this observation suggested that species of aeromonas, like those of other genera with inducible beta-lactamases, may pose therapeutic problems associated with the rapid development of multiple beta-lactam resi ...19883264129
effect of recent antibacterial agents against bacteria causing diarrhoea.the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...19883266900
gene probes for the detection of aerolysin in aeromonas spp. 19883269364
proceedings of the second aeromonas/plesiomonas workshop. miami beach, florida, usa, 7 may 1988. 19883269365
characterization of an aeromonas hydrophila strain isolated on a septicemic out-break in a fish-farm of spain.an aeromonas hydrophila strain (ah-3) was isolated from a septicemic out-break on a gold-fish farm near barcelona (spain). on the bases of their virulence and surface characteristics was classified as moderate to weakly virulent.19883271064
aeromonas hydrophila infections in children. 19883272526
the growth of aeromonas hydrophila k144 in ground pork at 5 degrees c.the influence of nacl, ph, atmosphere, and background microflora on the growth and/or survival of aeromonas hydrophila k144 was studied in ground pork held at 5 degrees c. in ground pork, a hydrophila was sensitive to ph values below 6.0 in the form of either a low starting ph in the pork itself or induced by lactic acid bacteria action on added glucose. growth of the organism is inhibited by nacl levels of 3% (w/w) (approx. 4% brine content). a hydrophila grew in vacuum-packaged ground pork; it ...19883275309
surface protein composition of aeromonas hydrophila strains virulent for fish: identification of a surface array protein.the surface protein composition of members of a serogroup of aeromonas hydrophila which exhibit high virulence for fish was examined. treatment of whole cells of representative strain a. hydrophila tf7 with 0.2 m glycine buffer (ph 4.0) resulted in the release of sheets of a tetragonal surface protein array. sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis analysis showed that this sheet material was composed primarily of a protein of apparent molecular weight 52,000 (52k protein) ...19883276660
tigemonam, an oral monobactam.tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ...19883279906
molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases.we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ...19883280033
aeromonas hydrophila gas gangrene: a case report of management with surgery and hyperbaric oxygenation. 19883282228
pneumonia due to aeromonas hydrophila-complex: epidemiologic, clinical, and microbiologic features.review of published data examining the various types of infections produced by strains of the aeromonas hydrophila-complex demonstrates a paucity of information on lower respiratory tract infections due to this organism. although it is rarely cited as a cause of pneumonitis, we have been able to collect epidemiologic, clinical, and microbiologic data on eight patients who have had evidence of aeromonas pneumonia. interestingly, seven of the eight patients were male, and the average age for the g ...19883282317
specific inhibition of aspartase by s-2,3-dicarboxyaziridine.aspartase of escherichia coli was inhibited in a competitive manner by s-2,3-dicarboxyazirdine (dcaz), an antibacterial substance against aeromonas salmonesida. the inhibition constant (ki) was 55 microm, which was as low as less than one tenth that of the km value for the substrate, l-aspartate. in view of the fact that both aspartase and fumarase (j. greenhut et al. (1985) j. biol. chem. 260, 6684-6686) were inhibited by dcaz in competitive manners, common features of the reaction mechanism of ...19883289541
partial purification and characterization of an extracellular proteinase from aeromonas hydrophila strain a4.an extracellular metalloproteinase from aeromonas hydrophila strain a4, isolated from milk, was purified by a factor of 300 by chromatography on deae-cellulose and sephadex g-150. the enzyme had a mol. wt of 43,000 and contained 2 g atom ca/mol. it was active over a ph range 4.8-9.5 and had optimum activity on casein at ph 7.0 with km = 0.17 mm. it was strongly inactivated by metal chelators and the apoenzyme was fully reactivated with ca2+, mn2+ or co2+. heavy metal ions such as ag+, hg2+, fe2+ ...19883290294
a comparative study of the formation of extracellular proteins by aeromonas salmonicida at two different temperatures.aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees c, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees c, the optimum temperature for growth. similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees c and 18 h at 25 degrees c. at this point, a ...19873298187
in vitro activity and beta-lactamase stability of a new monobactam, b0-1165.b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ...19873300528
[study of the sensitivity of enteropathogenic bacteria to nifurzide].nifurzide, a synthetic antimicrobial agent of the nitrofuran group, was tested on 320 enteropathogenic bacteria. minimal inhibition concentrations (mic) (expressed in mg/l) were determined using the standard agar dilution technique. the mics were excellent for campylobacter spp. (97 strains), mic 50:0.5, mic 90:0.5; slightly higher for shigella spp. (50 strains) and aeromonas spp. (22 strains), mic 50:2, mic 90:4; and highest for salmonella spp. (100 strains) and yersinia enterocolitica (49 stra ...19873302859
marker exchange mutagenesis of the aerolysin determinant in aeromonas hydrophila demonstrates the role of aerolysin in a. hydrophila-associated systemic infections.we report here on the isolation of isogenic strains of aeromonas hydrophila ab3 deleted for a segment of the aerolysin gene. all aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for chinese hamster ovary tissue culture cells. one such mutant, ab3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of a. hydrophila infections. the strain had a 50% lethal dose (ld50) of great ...19873305370
marine bacteria which produce tetrodotoxin.a number of type strains of marine bacteria, including members of the family vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. most of the vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both.19873310884
natural microbiologic hazards of the aquatic environment. 19873311341
[tte-ras (titertek-enterobac-ras), a new micromethod for the semi-automatic identification of enterobacteriaceae within 5 hours].titertek-enterobac-ras (tte-ras), a new semi-automated system for the identification of the enterobacteriaceae-within five hours, has been evaluated and compared with conventional methods. this note presents the results upon 655 strains, mainly enterobacteriaceae tte-ras provided correct identification for about 92 p. cent and 97 p. cent of enterobacteriaceae respectively before and after carrying out supplementary tests according to manufacturer's instruction. tte-ras gave 97 p. cent specific r ...19873314607
[aeromonas hydrophila as the causative agent of ileocolitis]. 19873315547
[aeromonas spp. as pathogens in human infections: diagnosis and clinical significance].aeromonads have been described as causative agents in a wide variety of infections in immunosuppressed as well as in immunocompetent patients. it is still controversial whether or not some or all three currently recognized species have to be considered as enteropathogenic. clinical and epidemiological data are incomplete and contradictory although laboratory diagnosis does not cause real problems.19873315971
does aeromonas hydrophila preferentially colonize the bowels of patients with hematologic malignancies?weekly cultures of stools from neutropenic patients and bone marrow transplant recipients yielded aeromonas hydrophila from 8% of 88 patients over a 2-yr period. during this time stools in the routine enteric laboratory yielded a. hydrophila in 0.24% of 1632 patients. although the patient groups and culture methods were not directly comparable, this significant difference in isolation rate (p less than 0.001) may reflect a higher colonization rate in the immunocompromised patient.19873319373
a comparison of the distribution of extracellular proteins produced by the protease-secreting organism aeromonas salmonicida during aerobic and anaerobic growth.aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees c. although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. however, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. th ...19863322167
[fatal aeromonas septicemia in two patients with acute myelogenous leukemia: clinical significance in neutropenic states and review of the literature]. 19873323564
evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.a small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of enterobacteriaceae, 15 strains of aeromonas sp., and 3 strains of plesiomonas shigelloides. the system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. ninety strains (18.6%) were not identified. only 11 str ...19873326369
phenotypic differentiation of members of the family vibrionaceae using miniaturized biochemical tests.enzymatic hydrolysis of 27 different chromogenic substrates and the assimilation of 44 carbon sources by 144 strains of vibrio species of clinical importance, aeromonas hydrophila and plesiomonas shigelloides were studied by standardized micromethods. some classical biochemical tests were also performed using the test kit tte-as (flow laboratories gmbh, meckenheim, frg). reading of results was done automatically by a photometer and test data were recorded and stored by a microcomputer. all speci ...19873326370
[a case of aeromonas hydrophila sepsis with generalized gas-formation and intravascular hemolysis]. 19873334425
antimicrobials and fish: a review of drugs used to treat bacterial diseases of channel catfish and rainbow trout.the principal bacterial diseases of cultured rainbow trout (salmo gairdneri) and channel catfish (ictalurus punctatus), and the antibacterials used in their treatment are briefly reviewed. gram-negative bacteria predominate, and oxytetracycline is effective in treatment of infections induced by them. the only antibacterials now registered by the us food and drug administration are sulfamerazine for the treatment of furunculosis in trout, and oxytetracycline for the treatment of furunculosis in s ...19863334692
aeromonas-associated gastroenteritis in children.during a 20-month period 55 strains of aeromonas species were isolated from 53 children with diarrhea. the isolation rate of 2.5% for aeromonas compared with the rates of 4.5% for shigella, 3.3% for salmonella, 2.7% for campylobacter and 0.05% for yersinia. in 45 children aeromonas was the sole bacterial enteropathogen identified. aeromonas was also isolated from 2 (0.5%) of 380 asymptomatic children. despite its known lack of identifiable virulence properties, aeromonas caviae was the most prev ...19883340460
aeromonas hydrophila wound infection in a patient with cyclic neutropenia following a piranha bite. 19883340462
effect of incubation temperature on growth and soluble protein profiles of motile aeromonas strains.motile aeromonas spp. are said to have an optimal growth temperature of 28 degrees c. we performed growth studies on 24 isolates at 29 and 37 degrees c and found that the optimal growth temperature was not necessarily 28 degrees c. we also detected temperature-dependent differences in soluble protein production in some of these strains.19883343335
production of monoclonal antibody against aeromonas hydrophila haemolysin.two hybridoma cell lines that produce monoclonal antibodies against aeromonas hydrophila haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitromethane. enzyme-linked immunosorbent assay (elisa) showed that the two purified monoclonal antibodies, b7 and b11, recognised the same epitope on the haemolysin molecule. antibody b7 neutralised the haemolytic and enterotoxic activities of the haemolysin. it is concluded ...19883346901
[prevalence of aeromonas spp. pediatric gastroenteritis].a selective medium and biochemical tests were used to search for aeromonas spp. in the stools of 536 children, more than 90% of whom had "gastroenteritis", seen at sainte-justine hospital, montreal, in june or july 1986. the organism was found in seven children, six of whom had bloody diarrhea. one of the six, a 6 1/2-year-old boy, required intravenous alimentation and later manifested ulcerative colitis; the other five recovered. the remaining child, a 14-year-old boy, had synovitis of the knee ...19883355951
gas-liquid chromatographic analysis of fatty acid methyl esters of aeromonas hydrophila, aeromonas sobria, and aeromonas caviae.clinical isolates of aeromonas hydrophila, a. sobria, and a. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (glc) displayed the following qualitatively similar glc profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-oh 14:0. the 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed ...19883366862
structure of an s layer on a pathogenic strain of aeromonas hydrophila.negative staining revealed a tetragonal surface array (s layer) on all the members of a serogroup of aeromonas hydrophila which possess high virulence for fish. the s layers were similar on all the strains examined, with unit cell dimensions of approximately 12 nm. a single representative strain, strain tf7, was selected for further analysis. freeze-cleaved and etched preparations and sections for electron microscopy showed that the s layer was the outermost component of the cell envelope. this ...19883372478
isolation and biochemical characterization of the s-layer protein from a pathogenic aeromonas hydrophila strain.the regular surface protein array (s layer) present on aeromonas hydrophila tf7 is composed of a single species of protein of apparent molecular weight 52,000. this protein was extracted from whole cells by treatment with 0.2 m glycine hydrochloride (ph 3.0). the protein was purified to homogeneity by ion-exchange chromatography and reverse-phase high-performance liquid chromatography. amino acid composition analysis showed that the protein contained 520 residues per molecule, 41% of which were ...19883372479
isolation of aeromonas salmonicida from masu salmon in the republic of korea.the isolation and identification of the gram-negative fish pathogen, aeromonas salmonicida salmonicida, from masu salmon (oncorhynchus masou) at the yang yang salmon hatchery in the republic of korea is described. the bacterium possessed certain characteristics in common with isolates from fish in japan. it is an important cause of mortality among cultured salmonids in the republic of korea. this is the first report of this fish pathogen from the asian mainland.19883373645
preparation of metal-free enzymes. 19883374375
screening of bacteria with antiviral activity from fresh water salmonid hatcheries.bacteria isolated from two salmonid hatcheries were screened for antiviral activity against infectious hematopoietic necrosis virus (ihnv) to ascertain the presence of bacteria with anti-ihnv activity in the aquatic environment. out of 710 bacterial isolates from the water and sediment samples, 190 strains showed anti-ihnv activities of more than 50% plaque reduction. these antiviral activities were detected predominantly in pseudomonas, aeromonas/vibrio, and coryneforms. in one hatchery, the ba ...19883374405
an unusual case of pyogenic meningitis due to aeromonas sobria. 19883382842
aeromonas hydrophila cellulitis. a case report. 19883385616
the phylogenetic origin of the bifunctional tyrosine-pathway protein in the enteric lineage of bacteria.because bifunctional enzymes are distinctive and highly conserved products of relatively infrequent gene-fusion events, they are particularly useful markers to identify clusters of organisms at different hierarchical levels of a phylogenetic tree. within the subdivision of gram-negative bacteria known as superfamily b, there are two distinctive types of tyrosine-pathway dehydrogenases: (1) a broad-specificity dehydrogenase (recently termed cyclohexadienyl dehydrogenase [cdh]) that can utilize ei ...19883386529
the effects of gamma irradiation on the lymphoid organs of rainbow trout and subsequent susceptibility to fish pathogens.fish were irradiated with 60co gamma rays at doses ranging from 10 to 50 gy. lethal doses were determined in fishes of different ages. for a given dose, fry and fingerlings were more susceptible than subadults. whatever the irradiation dose was, the fish displayed a sharp decrease in blood leucocyte count. at the lowest doses, this acute leucopaenia was reversible. the cellular damage in the lymphoid organs was particularly obvious in the thymus. the depletion of lymphoid cells from immunocompet ...19883388762
[analysis of degradative plasmids by pyrolysis gas liquid chromatography]. 19883388828
aeromonas eucrenophila species nova aeromonas caviae a later and illegitimate synonym of aeromonas punctata.aeromonas caviae is a later and illegitimate synonym of aeromonas punctata. in order to secure continuity in literature, it is proposed to use the trivial term "caviae type" in connection with the species name a. punctata temporarily especially for strains isolated in connection to diarrhoea in children. aeromonas eucrenophila sp. nov. is described, type strain in ncmb 74. the new aerogenic psychrotrophic aeromonas species is separated genotypically from aeromonas hydrophila and aeromonas puncta ...19883394446
oxolinic acid in the trout: bioavailability and tissue residues.a study was performed on the serum bioavailability and tissue elimination of oxolinic acid in the trout. the compound was added to the diet and administered at a dosage-level of 12 mg/kg/day for 7 consecutive days. the study utilized an analytical technique, high performance liquid chromatography, which has been described in detail here. the results obtained demonstrate that serum concentrations higher than the mic for the control of the target pathogens (aeromonas and yersinia) can by sustained ...19883400972
[frequency of aeromonas species in the stools of healthy and diarrheic patients].during two test periods (summer 1985 and january-august 1986) a total of 7713 stool specimens were examined for the occurrence of aeromonas spp. the isolation rate of the asymptomatic group was about 0.3%, the same rate was found in adults with diarrhea. in the stool specimens of 364 children 8 aeromonas spp. could be found. a. caviae was isolated in most cases of diarrhea. data obtained suggest that it seems to be useful to screen all diarrhoic stools of children for the occurrence of aeromonas ...19883402999
surface properties of autoagglutinating mesophilic aeromonads.the surface characteristics of 24 autoagglutinating (aa+) mesophilic aeromonads were investigated. one group of 16 was found to be highly related serologically by their reactive pattern against o antisera generated against three reference strains. subsequent characterization of 11 of these isolates (group 1) indicated that they had the following properties in common: precipitation after boiling (pab+), membership of serogroup o:11 (typing scheme of sakazaki and shimada), resistance to lysis by b ...19883417353
[antibiotic resistance of several gram-negative bacteria from clinical and dissection material from the institute for poultry diseases of ludwig maximilian university, munich].a survey is given on antibiograms carried out with important bacterial strains isolated from birds during 1987. as compared with the results from earlier years no significant changes could be shown, the sensitivity ranging from 42.4% to 95.9%. the genera bordetella and aeromonas were included for the first time.19883420603
the effect of different brands of macconkey and xylose lysine deoxycholate agar on the isolation of aeromonas hydrophila.aeromonas hydrophila has been reported to be involved in the opportunistic infection of human beings, therefore, its rapid diagnosis from the clinical specimens was considered to be extremely important for the prognosis. the effect of different brands of macconkey (mac) and xylose lysine deoxycholate (xld) agars on the plating efficiency and colony size of a. hydrophila was evaluated by comparing with blood agar. results indicate that (i) except eiken's mac have low plating efficiency, all the m ...19873428016
enteric pathogens in tropical aquaria.a total of 100 tropical aquaria from six pet shops were sampled for the presence of salmonella, campylobacter and aeromonas species. eight fish tanks contained unusual serotypes of salmonella. none had been used to house terrapins. one hundred and three isolates of aeromonas species were obtained from 98 tanks. one tank contained plesiomonas shigelloides. no campylobacter species were isolated. it is suggested that a tropical aquarium may constitute an infection hazard.19873428370
lymphadenopathy syndrome and hiv infection in multitransfused beta-thalassemia child.lymphoadenopathic syndrome (las), diagnosed also with histologycal studies, is described in a polytransfused 11-year old boy affected by beta-thalassemia major. after three days of the admission he suffered a serious acute diarrhea by aeromonas hydrophila, an opportunistic enteric bacteria demonstrated, up to date, in immunocompromised patients and never in aids patients. it is important to remark that beta-thalassemic patients are at high risk from hiv infections, either for the chronic need of ...19873435653
[spontaneous bacterial peritonitis caused by aeromonas hydrophila. presentation of 2 cases]. 19873438545
two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ...19873440458
[geographic variations of the isolation of aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population]. 19873444950
bacterial flora in bottled uncarbonated mineral drinking water.a quantitative study of bacterial populations in mineral water was carried out. samples were stored at 6 and 20 degrees c, and the colony counts were determined on tryptone agar plates incubated at 22 and 37 degrees c. samples were collected from the spring source in sterile glass flasks and from the bottling factory in conventional plastic and glass containers. in both cases, the initial population (10(1)-10(2) cfu/ml water) increased to 10(5)-10(6) cfu/ml after 3 days storage as determined fro ...19873446349
[pulmonary abscess: aeromonas hydrophila caused by a complication of drowning in fresh water]. 19873450216
aeromonas infection following facial injury. 19863459840
bacteremia due to aeromonas hydrophila: a report of 40 episodes. 19863461126
activity of newer antimicrobial agents against aeromonas hydrophila. 19863463508
the in-vitro activity of ci-934 compared with that of other new 4-quinolones and nalidixic acid.the in-vitro activity of ci-934, a new 4-quinolone compound, was compared with that of the other new 4-quinolones, enoxacin and ciprofloxacin, and also with that of nalidixic acid. ci-934 was more active than any of the other 4-quinolones tested against gram-positive aerobic organisms including staphylococcus aureus (mics 0.06-0.25 mg/l), beta-haemolytic streptococci (mics 0.12-0.5 mg/l), streptococcus pneumoniae (mics 0.25-0.5 ml/l), viridans streptococci (mics 0.06-0.5 mg/l) and most enterococ ...19863463557
the comparative in-vitro activity of eight newer quinolones and nalidixic acid.the in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, a-56619, a-56620 and ci-934 was assessed by determination of mics. the 4-quinolones were all highly active against most isolates of enterobacteriaceae, including nalidixic acid-resistant strains. ciprofloxacin (mics 0.002-2 mg/l) was the most active and a-56619 (mics 0.008-32 mg/l) was the least active. a-56619, a-56620, ofloxacin, ciprofloxacin and ci-934 were ...19863468100
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
in vitro activity of ci-934 compared with ciprofloxacin, enoxacin, norfloxacin, and vancomycin.the in vitro activity of the quinolone ci-934 was compared with ciprofloxacin, norfloxacin, enoxacin, and vancomycin against 607 gram-positive and -negative isolates. ci-934 inhibited 90% of the enterobacteriaceae, aeromonas hydrophila, and acinetobacter spp. at 1 microgram/ml. decreased activity was observed against pseudomonas aeruginosa. pseudomonas maltophilia, and other pseudomonas spp. with ci-934 mic90 greater than or equal to 8 micrograms/ml. ci-934 activity against gram-positive organis ...19873472704
cutaneous infections of the coast. 19873480441
beta-lactamases with high activity against imipenem and sch 34343 from aeromonas hydrophila.two groups of inducible beta-lactamases were found among nine isolates of aeromonas hydrophila. five isolates produced enzymes with high activity against the penem sch 34343 and the carbapenem imipenem: the enzymes from some of these isolates also had activity against penicillin, ampicillin, carbenicillin, cephaloridine and cephalexin but one isolate produced an enzyme with no detectable activity against cephalosporins. the other four isolates produced typical 'cephalosporinases' with activity a ...19863485091
synergism and antagonism in double beta-lactam antibiotic combinations.combinations of two beta-lactam antibiotics may be advantageous in certain clinical situations, providing a synergistic activity against specific organisms or a broad spectrum of antibiotic coverage. depending on the combination and the bacteria, synergism, indifference, or antagonism can be observed. synergism may occur when two beta-lactam antibiotics, acting on different penicillin-binding proteins, are combined or when a penicillinase-susceptible beta-lactam antibiotic is protected by anothe ...19863487247
in vitro activity and beta-lactamase stability of two oral cephalosporins, ceftetrame (ro 19-5247) and cefetamet (ro 15-8074).ceftetrame (ro 19-5247) and cefetamet (ro 15-8074), two new orally administered aminothiazolyl imimomethoxy cephalosporins, inhibited hemolytic streptococci and streptococcus pneumoniae at less than or equal to 0.5 micrograms/ml but were less active against staphylococci than were cephalexin and cefaclor. they did not inhibit s. faecalis, s. faecium, listeria monocytogenes, corynebacterium jk species, or pseudomonas aeruginosa. haemophilus influenzae, branhamella catarrhalis, and neisseria gonor ...19863490827
in vitro activity and beta-lactamase stability of a new penem, cgp 31608.the in vitro activity of cgp 31608, a new penem, against aerobic and anaerobic organisms was evaluated and compared with those of other beta-lactams. cgp 31608 inhibited escherichia coli, klebsiella pneumoniae, k. oxytoca, proteus mirabilis, citrobacter diversus, and salmonella, shigella, aeromonas, and yersinia spp. with mics for 50% of the strains (mic50s) of 2 to 4 micrograms/ml and mic90s of 4 micrograms/ml, compared with cefotaxime, ceftazidime, aztreonam, and imipenem mics of less than 0.2 ...19873496845
gram-negative septicemia in american alligators (alligator mississippiensis).six species of bacteria (family enterobacteriaceae) not commonly reported as associated with disease in american alligators (alligator mississippiensis) were documented, suggesting that aeromonas is not the only bacterium responsible for septicemia in crocodilians. these included citrobacter freundii, enterobacter agglomerans, proteus sp., morganella morganii, serratia marcescens, and klebsiella oxytoca. clinical signs of disease included intensive basking, anorexia, lethargy, flaccid limb paral ...19863503133
binding of clavulanic acid to the penicillin-binding proteins of aeromonas hydrophila. 19873509501
stress factors that can affect studies of drug metabolism in fish.the effects of environmental stress conditions on the defense response of rainbow trout following a four week exposure to subacute levels of un-ionized ammonia or temperatures 5 c above and 5 c below the temperature optimum (15 c) were investigated. these experimental studies can serve as a model to evaluate the metabolic response of fish to external agents (e.g., drugs, vaccines) under environmental conditions seen in the culture of fish. blood and tissue immune parameters measured include hema ...19863509652
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