Publications

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multiresistant waterborne pathogens isolated from water reservoirs and cooling systems.to determine the incidence of multiple antibiotic-resistant strains of the emergent human pathogens legionella pneumophila, pseudomonas aeruginosa and mesophilic aeromonas species among those isolated from water reservoirs and industrial cooling systems.200818298535
cortisol response and immune-related effects of atlantic salmon (salmo salar linnaeus) subjected to short- and long-term stress.it is generally considered that stress causes decreased immune function in fish. in this study we examined in atlantic salmon (salmo salar linnaeus) the effects of both short- (a single 15s out of water) and long-term (4 weeks of daily handling 15s out of water) stress on plasma cortisol (free and total) and glucose levels, expression of interleukin-1beta (il-1beta) and survival of head kidney (hk) macrophages under culture with aeromonas salmonicida. in the short-term study, samples were collec ...200818065240
quorum sensing by n-acylhomoserine lactones is not required for aeromonas hydrophila during growth with organic particles in lake water microcosms.it was investigated whether quorum sensing (qs) mediated by n-acylhomoserine lactones (ahls) was important for heterotrophic bacteria from the littoral zone of the oligotrophic lake constance for growth with organic particles. more than 900 colonies from lake water microcosms with artificial organic aggregates consisting of autoclaved unicellular algae embedded in agarose beads were screened for ahl-production. ahl-producing bacteria of the genus aeromonas enriched in the microcosms but ahls cou ...200818066531
the involvement of aeromonas salmonicida virulence factors in bacterial translocation across the rainbow trout, oncorhynchus mykiss (walbaum), intestine.the pathogenic bacterium aeromonas salmonicida is the causative agent of furunculosis, a lethal disease in salmonids. the mode of lateral transmission has not been conclusively defined, but a. salmonicida is able to translocate across the intestinal epithelium of salmonids, making the intestinal route a probable candidate. this study investigated some of the virulence mechanisms used by the bacteria to promote translocation. intestinal segments were placed in modified ussing chambers to investig ...200818234022
inactivation of host akt/protein kinase b signaling by bacterial pore-forming toxins.uropathogenic escherichia coli (upec) are the major cause of urinary tract infections (utis), and they have the capacity to induce the death and exfoliation of target uroepithelial cells. this process can be facilitated by the pore-forming toxin alpha-hemolysin (hlya), which is expressed and secreted by many upec isolates. here, we demonstrate that hlya can potently inhibit activation of akt (protein kinase b), a key regulator of host cell survival, inflammatory responses, proliferation, and met ...200818234841
application of antimicrobial ice for extending shelf life of fish.in this study, we investigated whether wild-thyme (thymus serpyllum) hydrosol had a preserving effect against spoilage of freshwater fish. sensorial characteristics, chemical freshness indicator contents, and microbial counts (total aerobes, psychrotrophics, enterobacteriaceae, fecal coliform bacteria, aeromonas spp., and pseudomonas spp.) of whole ungutted and gutted transcaucasian barb (capoeta capoeta capoeta guldenstaedt, 1772) stored on ice produced from wild-thyme hydrosol and tap water at ...200818236688
gene cloning, expression, and characterization of a second beta-n-acetylglucosaminidase from the chitinolytic bacterium aeromonas hydrophila strain suwa-9.a gene coding for a second beta-n-acetylglucosaminidase (nagb) was isolated from the chitinolytic bacterium aeromonas hydrophila strain suwa-9. the nagb open reading frame encoded a polypeptide of 618 amino acid residues with a molecular mass of 68.8 kda. it did not contain a sequence characteristic of a signal peptide at the n-terminus. the deduced amino acid sequence showed high similarity to those of bacterial beta-n-acetylhexosaminidases classified into family 20 of glycosyl hydrolases. the ...200818256480
extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation.the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ...200818256966
unexpected occurrence of plasmid-mediated quinolone resistance determinants in environmental aeromonas spp.we searched for plasmid-mediated quinolone resistance determinants of the qnr type in several water samples collected at diverse locations from the seine river (paris, france). the qnrs2 genes were identified from aeromonas punctata subsp. punctata and a. media. the qnrs2 gene was located on incu-type plasmids in both isolates, which resulted in increased mic values of quinolones and fluoroquinolones, once they were transferred into escherichia coli. the qnrs2 gene identified in a. punctata was ...200818258115
characterization of vibrio cholerae isolated from the aquatic basins of the state of pernambuco, brazil.through a continuous bacteriological monitoring programme carried out by the health secretariat of the state of pernambuco, brazil, two isolates of vibrio cholerae o1 el tor ogawa were discovered in an endemic area in 2001, during a cholera inactive period, along with six v. cholerae non-o1/non-o139 strains and two aeromonas veronii biovar sobria strains showing an unusual characteristic of agglutination with o1 antiserum. between that time and 2005, eight other o1 isolates were found. the virul ...200818258274
biokinetic parameters and behavior of aeromonas hydrophila during anaerobic growth.the biokinetics of glucose metabolism were evaluated in aeromonas hydrophila during growth in an anaerobic biosystem. after approx 34 h growth, a. hydrophila metabolized 5,000 mg glucose l(-1) into the end-products ethanol, acetate, succinate and formate. the maximum growth rate, mu(m), half saturation coefficients, k(s), microbial yield coefficient, y, cell mass decay rate coefficient, k(d), and substrate inhibition coefficient, k(si) were 0.25 +/- 0.03 h(-1), 118 +/- 31 mg glucose l(-1), 0.12 ...200818265942
role of the aheabc efflux pump in aeromonas hydrophila intrinsic multidrug resistance.gene inactivation and complementation experiments showed that the tripartite aheabc efflux pump of aeromonas hydrophila extruded at least 13 substrates, including nine antibiotics. the use of phenylalanine-arginine-beta-naphthylamide (pabetan) revealed an additional system(s) contributing to intrinsic resistance. this is the first analysis of the role of multidrug efflux systems in aeromonas spp.200818268083
predictors of mortality in patients with necrotizing fasciitis.necrotizing fasciitis is an uncommon and life-threatening soft tissue infection with high mortality. though early aggressive surgical intervention is important for improving survival, the impact of mortality from different microorganisms remains uncertain. our study aims to identify the association of mortality and different microorganisms, and the positive and negative predictors of mortality in patients with necrotizing fasciitis.200818272096
recombinant production and characterization of the carbohydrate recognition domain from atlantic salmon c-type lectin receptor c (sclrc).the atlantic salmon c-type lectin receptor c (sclrc) locus encodes a potential oligomeric type ii receptor. c-type lectins recognize carbohydrates in a ca(2+)-dependent manner through structurally conserved, yet functionally diverse, c-type lectin-like domains (ctlds). many conserved amino acids in animal ctlds are present in sclrc, with the notable exception of an asparagine crucially involved in ca(2+)- and carbohydrate-binding, which is tyrosine in sclrc. sclrc also contains six cysteines tha ...200818272393
16s rrna gene sequences from bacteria associated with adult anopheles darlingi (diptera: culicidae) mosquitoes.the microbial flora associated with anopheles darlingi root (diptera: culicidae), a major neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in brazil. female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. the controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial dna from all blood-fed m ...200818283961
cold shock exoribonuclease r (vacb) is involved in aeromonas hydrophila pathogenesis.in this study, we cloned and sequenced a virulence-associated gene (vacb) from a clinical isolate ssu of aeromonas hydrophila. we identified this gene based on our recently annotated genome sequence of the environmental isolate atcc 7966(t) of a. hydrophila and the vacb gene of shigella flexneri. the a. hydrophila vacb protein contained 798 amino acid residues, had a molecular mass of 90.5 kda, and exhibited an exoribonuclease (rnase r) activity. the rnase r of a. hydrophila was a cold-shock pro ...200818344363
evaluation of reca sequencing for the classification of aeromonas strains at the genotype level.to evaluate the usefulness of partial reca sequences for the identification of aeromonas strains at the genotype level.200818346137
development and application of real-time pcr assays for quantifying total and aerolysin gene-containing aeromonas in source, intermediate, and finished drinking water.aeromonas spp., opportunistic pathogens, are listed as a microbiological contaminant on the environmental protection agency's (epa) drinking water contaminant candidate list. culture-based methods for identification and quantification of aeromonas in drinking water are time-consuming and often fail to differentiate pathogenic species from nonpathogenic ones. this study reports successful development and applications of two real-time pcr assays, based on 16s rrna gene sequences and a virulence ge ...200818351092
identification of bacteria from the normal flora of perch, perca fluviatilis l., and evaluation of their inhibitory potential towards aeromonas species.pathogenic aeromonas sobria has been identified as a causative agent of ulcerative disease in farmed european perch, perca fluviatilis l. to study the effect of the normal intestinal bacterial flora of perch against a. sobria, we sampled 193 bacterial isolates from the perch digestive tract. the isolates were identified by sequence analysis of the 16s rrna gene and their inhibitory potential against a. sobria was evaluated in vitro. nineteen of the strains isolated showed inhibition and were als ...200818355180
chemotaxis towards, adhesion to, and growth in carp gut mucus of two aeromonas hydrophila strains with different pathogenicity for common carp, cyprinus carpio l.characteristics that promote bacterial colonization of the intestinal mucosal surface were examined in two strains of the common fish pathogen aeromonas hydrophila, with different pathogenicity. the characteristics examined were chemotactic activity towards mucus, bacterial adherence to mucus and growth in mucus. intestinal gut mucus of healthy common carp was used. the results indicate that chemotaxis is not necessary for a bacterium to become pathogenic, but it may be a necessary parameter for ...200818355183
degradation of linear alkylbenzene sulfonate by a bacterial consortium isolated from the aquatic environment of argentina.to isolate and identify linear alkylbenzene sulfonate (las)-degrading bacteria from río de la plata and adjacent waters, and to assay their degradation capability as a consortium and as single organisms.200818355233
effect of packaging atmosphere on the microbial attributes of pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c.effect of packaging atmosphere (air and under different modified atmospheres (mas), 40% co2/60% o2, 50%/50% o2, 60% co2/40% o2, 70% co2/30% o2 and 40% co2/30% o2/30% n2) on the microbial and biochemical attributes of fresh pearlspot (etroplus suratensis bloch) stored at 0-2 degrees c was investigated. trimethylamine nitrogen (tma-n) and thiobarbituric acid (tba) values remained lower than the proposed acceptability limits throughout the storage period. results demonstrated that storage of pearls ...200818355677
identification of the first vim metallo-beta-lactamase-producing multiresistant aeromonas hydrophila strain.a vim metallo-beta-lactamase-producing aeromonas hydrophila strain carrying an integron-borne bla(vim-4) gene was isolated from a cirrhotic patient's fecal sample in a budapest hospital. the variable region of this integron is identical with that of a previously characterized integron from pseudomonas aeruginosa clinical isolates in pécs in southern hungary.200818367570
transferable, multiple antibiotic and mercury resistance in atlantic canadian isolates of aeromonas salmonicida subsp. salmonicida is associated with carriage of an inca/c plasmid similar to the salmonella enterica plasmid psn254.the aim of this study was to examine the molecular basis for multiple antibiotic and mercury resistance in canadian isolates of aeromonas salmonicida subsp. salmonicida.200818375380
[microbiological aspects of pool water].it is well known that there is an elevated risk of diffusion of pathogenic micro-organisms in swimming pools. preventive measures aimed at protecting the health of swimmers are complex because of the variety of micro-organisms involved, the different ways in which these can be transmitted and the involvement of both aquatic and environmental factors. in industrialised countries, the circulation of many pathogens which were common in the past has progressively decreased in recent years; in contra ...200818379609
necrotizing skin and soft-tissue infections associated with septicemia: 7 cases report and review.the authors report seven cases of necrotizing skin and soft-tissue infections, with clinical presenting as hemorrhagic bullae, gangrenous cellulitis or necrotizing fasciitis, in association with septicemia, between january 2003 and january 2007 in hat yai hospital. six were male and the majority of the lesions, six cases, occurred in the lower extremities. the average age of the patients was 50.0 +/- 11.019 years old. all patients presented with watery diarrhea, severe abdominal pain, high fever ...200818386555
prevalence of bacterial pathogens and their anti-microbial resistance in tilapia and their pond water in trinidad.in trinidad, tilapia (oreonchromis spp.) is one of the most important fresh water food fish and the number of farms has been increasing annually. a study was conducted in the local tilapia industry to determine the microbial quality of pond water, prevalence of bacterial pathogens and their anti-microbial resistance using the disk diffusion method. seventy-five apparently healthy fish and 15 pond water samples from three of the four commercial tilapia fish farms in the country were processed. th ...200818387142
pathogenic potential of a collagenase gene from aeromonas veronii.the role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. the information on the role of collagenase in aeromonas spp. pathogenesis is scant. in the present study, a mutant aeromonas veronii ry001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, ...200818388966
cloning, expression, and transglycosylation reaction of paenibacillus sp. strain w-61 xylanase 1.a xylanase gene, xyn1, which encodes paenibacillus sp. strain w-61 xylanase 1 (xyn1), was cloned in escherichia coli. xyn1 encodes 211 amino acid residues, including 28 amino acid residues of a signal peptide. the deduced amino acid sequence of the mature xyn1 showed 95.7%, 84.0%, and 83.7% identity to family 11 xylanases of aeromonas caviae me-1, paenibacillus sp., and bacillus stearothermophilus respectively. the physico-chemical properties of recombinant xyn1 were very similar to those of int ...200818391461
cloning, binding properties, and tissue localization of rainbow trout (oncorhynchus mykiss) ladderlectin.the present paper describes the primary structure, expression and immunohistochemical localization of rainbow trout ladderlectin (rtll), a multimeric serum lectin that binds sepharose and lps of aeromonas salmonicida. two rainbow trout cdnas (504 and 546bp) and a genomic sequence (2kb) were amplified using ladderlectin-specific primers. the sequences were identified as group vii mannose-binding c-type lectins from predicted amino acid sequences and showed highest identity with the atlantic salmo ...200818407519
the aeromonas hydrophila wb*o34 gene cluster: genetics and temperature regulation.the aeromonas hydrophila wb*(o34) gene cluster of strain ah-3 (serotype o34) was cloned and sequenced. this cluster contains genes necessary for the production of o34-antigen lipopolysaccharide (lps) in a. hydrophila. we determined, using either mutation or sequence homology, roles for the majority of genes in the cluster by using the chemical o34-antigen lps structure obtained for strain ah-3. the o34-antigen lps export system has been shown to be a wzy-dependent pathway typical of heteropolysa ...200818408022
complete sequence of the flor-carrying multiresistance plasmid pab5s9 from freshwater aeromonas bestiarum.a multiresistant aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location and organization of antimicrobial resistance genes.200818413319
aeromonas hydrophila bacteraemia and portal pyaemia.the aeromonas species uncommonly cause disease in humans. we report portal pyaemia secondary to aeromonas hydrophila bacteraemia occurring in a 71-year-old chinese man with no history of hepatobiliary disease or malignancy. he presented with fever, rigors and abdominal bloating for four days and was subsequently found to have aeromonas hydrophila bacteraemia, portal vein thrombosis and a psoas abscess. he was treated with ciprofloxacin and had a good recovery. aeromonas hydrophila infection is a ...200818418529
efficacy of in-feed probiotics against aeromonas bestiarum and ichthyophthirius multifiliis skin infections in rainbow trout (oncorhynchus mykiss, walbaum).the aim of this study was to assess the efficacy of in-feed probiotics as a preventive measure against skin infections caused by aeromonas bestiarum and ichthyophthirius multifiliis (ich) in rainbow trout.200818422955
comparing the antimicrobial effectiveness of nacl and kcl with a view to salt/sodium replacement.a study using a small range of pathogenic bacterial species (aeromonas hydrophila, enterobacter sakazakii, shigella flexneri, yersinia enterocolitica and 3 strains of staphylococcus aureus) has shown that potassium chloride has an equivalent antimicrobial effect on these organisms when calculated on a molar basis. combined nacl and kcl experiments were carried out and data was analysed using a modification to the lambert and lambert [lambert, r.j.w., and lambert, r., 2003. a model for the effica ...200818423764
development and application of an oligonucleotide microarray and real-time quantitative pcr for detection of wastewater bacterial pathogens.conventional microbial water quality test methods are well known for their technical limitations, such as lack of direct pathogen detection capacity and low throughput capability. the microarray assay has recently emerged as a promising alternative for environmental pathogen monitoring. in this study, bacterial pathogens were detected in municipal wastewater using a microarray equipped with short oligonucleotide probes targeting 16s rrna sequences. to date, 62 probes have been designed against 3 ...200818423816
production of nitric oxide by carp (cyprinus carpio l.) kidney leukocytes is regulated by cyclic 3',5'-adenosine monophosphate.the inducible nitric oxide synthase (inos) plays a central role in the inflammatory reactions that follow infection or tissue damage. induction of nitric oxide (no) synthesis by bacterial lipopolysaccharide (lps) depends on activation of g protein-coupled receptors in mammals. thus, it was our intention to evaluate whether similar mechanisms are involved in inos activation in fish leukocytes. therefore, the participation of membrane-bound receptors which activate effectors via g proteins has bee ...200818424130
application of pcr-rf-sscp to study major histocompatibility class ii b polymorphism in common carp (cyprinus carpio l.).a variety of methods have been applied for the characterization of major histocompatibility (mh) polymorphism in fish. we optimized a technique designated polymerase chain reaction-restriction fragments-single strand conformation polymorphism (pcr-rf-sscp) for screening a large number of individuals for the cyca-dab1 and cyca-dab2 genes polymorphism in common carp. the advantages of this technique are simplicity, high sensitivity and low costs. pcr-rf-sscp analysis revealed different genotypes c ...200818430588
high-throughput screening of peptide deformylase inhibitors.the emergence of bacterial pathogens resistant to current antibiotics has caused an urgent demand for new treatments. peptide deformylase (pdf) has become an exciting target for designing novel antibiotics. to facilitate the screening of pdf inhibitors, three robust, coupled assays have been developed. the first method couples the pdf reaction with that of formate dehydrogenase. formate dehydrogenase oxidizes formate into co2 with a concomitant reduction of nad+ to nadh, which can be monitored s ...200818437310
[generation of aeromonas hydrophila ghosts and their evaluation as oral vaccine candidates in carassius auratus gibelio].the bacterial ghost (bg) system is a novel vaccine delivery system endowed with intrinsic adjuvant properties. bacterial ghosts are nonliving gram-negative bacterial cell envelopes devoid of cytoplasmic contents while maintaining their cellular morphology and native surface antigenic structures. they are produced by phix174 protein e-mediated lysis of gram-negative bacteria, and can induce humoral and cellular immune response, including mucosal immune responses. plasmid pelysis consisting e gene ...200818438002
molecular cloning, characterization and expression analysis of the pkz gene in rare minnow gobiocypris rarus.double-stranded rna-activated protein kinase (pkr) plays an important role in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. in the present study, a pkr-like gene was cloned and characterized from rare minnow gobiocypris rarus. the full length of the rare minnow pkr-like (grpkz) cdna is 1946 bp in length and encodes a polypeptide of 503 amino acids with an estimated molecular ma ...200818448359
comparison of atypical furunculosis vaccines in spotted wolffish (anarhicas minor o.) and atlantic halibut (hippoglossus hippoglossus l.).atypical furunculosis caused by atypical aeromonas salmonicida is a problem in farming of atlantic halibut (hippoglossus hippoglossus) and spotted wolffish (anarhicas minor) in norway, and vaccines for marine fish species are not available. susceptibility to atypical a. salmonicida infection and efficacy of furunculosis vaccines in various fish species indicate that the host responses differ. here, spotted wolffish was shown to be highly susceptible to intraperitoneal challenge with atypical a. ...200818450337
studies on bacillus subtilis and lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of tilapia nilotica (oreochromis niloticus) to challenge infections.the probiotic activity of two bacteria (bacillus subtilis and lactobacillus acidophilus) was evaluated by its effect on the immune response of nile tilapia (oreochromis niloticus), beside its protective effect against challenge infections. furthermore, their in-vitro inhibitory activity was evaluated. the in-vitro antimicrobial assay showed that bacillus subtilis and lactobacillus acidophilus inhibited the growth of a. hydrophila. the b. subtilis inhibited the development of p. fluorescens while ...200818450477
aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish.during a survey to determine the prevalence of aeromonas strains in water and skin of imported ornamental fish, 48 strains presumptively identified as aeromonas were isolated but they could not be identified as members of any previously described aeromonas species. these strains were subjected to a polyphasic approach including phylogenetic analysis derived from gyrb, rpod and 16s rrna gene sequencing, dna-dna hybridization, maldi-tof ms analysis, genotyping by rapd and extensive biochemical and ...200818450708
identification of mhc class iibeta resistance/susceptibility alleles to aeromonas salmonicida in brook charr (salvelinus fontinalis).pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (mhc) genes but have been tested for very few vertebrates. in this study, we first investigate by sscp (single strand conformational polymorphism) the diversity found at the mhc class iibeta gene in a population of brook charr (salvelinus fontinalis) from the rupert river (québec, canada). secondly, to explore the survival performances conferred by sp ...200818455800
the early response of atlantic salmon (salmo salar) macrophages exposed in vitro to aeromonas salmonicida cultured in broth and in fish.aeromonas salmonicida is a fish pathogen that causes furunculosis. virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. it is known that a. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. therefore, in this study, the early macrophage respon ...200817825909
a comparison of antibiotic resistance integrons in cattle from separate beef meat production systems at slaughter.to compare antibiotic resistance integrons in cattle from three separate grass-fed, grain-fed and certified organic cattle production systems at slaughter.200817927756
construction of a chimeric aminopeptidase by a combination of gene shuffling and mutagenesis.three chimeric genes were constructed by gene shuffling of aminopeptidases from aeromonas caviae and vibrio proteolyticus. although expressed chimeric enzymes formed inclusion bodies in escherichia coli, the introduction of two amino acid mutations into the chimeric genes by site-saturated mutagenesis and a random mutation on error-prone pcr resulted in solubilization of the chimeric enzyme. in addition, active chimeric enzyme showed a different thermostability and thermoactivity to parental enz ...200817934695
microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates.during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ...200817728184
aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains.aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. a total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in são paulo (brazil), were analyzed for the presence of aeromonas. samples were concentrated by membrane filtration and enriched in apw. ada medium was used for aeromonas isolation and colonies were confirmed by biochemical characterization. strains isolated w ...200817998612
drinking water microbiological survey of the northwestern state of sinaloa, mexico.a potable water survey, in two important municipalities of the state of sinaloa, mexico was conducted. culiacan, capital city of sinaloa and its neighboring municipality, navolato were selected to enumerate aeromonas hydrophila, escherichia coli, fecal and total coliforms, pseudomonas aeruginosa, and heterotrophic plate count bacteria from 100 households' taps. manganese; residual chlorine; ph; temperature and turbidity were also examined. overall, aeromonas hydrophila was not detected in any of ...200817998613
dietary korean mistletoe enhances cellular non-specific immune responses and survival of japanese eel (anguilla japonica).the present study was performed to investigate the immunostimulatory effects of korean mistletoe extract (km-110; viscum album coloratum) on the non-specific immune response and protection against aeromonas hydrophila infection in japanese eel (anguilla japonica). eels were fed under 4 regimes, 0%, 0.1%, 0.5% and 1.0% km-110 mixed diet. on day 14 after feeding, 15 fish from each group were injected i.p. with live a. hydrophila (3 x 10(6)cfu) and the remaining unchallenged fish from each group we ...200818023593
sensitive and rapid detection of aeromonas caviae in stool samples by loop-mediated isothermal amplification.in this study, a loop-mediated isothermal amplification assay targeting the 16s-23s intergenic spacer regions (internal transcribed spacer) of aeromonas caviae was developed. eighteen reference strains and 109 clinical samples were analyzed. the results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.200818024054
establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities.in this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ...200818034834
development of a pcr protocol for the detection of aeromonas salmonicida in fish by amplification of the fsta (ferric siderophore receptor) gene.the aims of the study were to evaluate a new pcr protocol designed to detect aeromonas salmonicida in fish tissues and to develop a non-destructive method for the diagnosis of furunculosis. a set of primers (fer3, fer4), flanking a fragment of the fsta gene (coding for the ferric-siderophore receptor) was designed, showing to be sensitive and specific. when compared to pcr methods previously reported, the new protocol recognized all the 69 a. salmonicida strains evaluated, with no cross-reaction ...200818035507
molecular characterization of a functional type vi secretion system from a clinical isolate of aeromonas hydrophila.our laboratory recently molecularly characterized the type ii secretion system (t2ss)-associated cytotoxic enterotoxin (act) and the t3ss-secreted aexu effector from a diarrheal isolate ssu of aeromonas hydrophila. the role of these toxin proteins in the pathogenesis of a. hydrophila infections was subsequently delineated in in vitro and in vivo models. in this study, we characterized the new type vi secretion system (t6ss) from isolate ssu of a. hydrophila and demonstrated its role in bacterial ...200818037263
proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets.the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ...200817981340
in vitro studies of lactobacillus delbrueckii subsp. lactis in atlantic salmon (salmo salar l.) foregut: tissue responses and evidence of protection against aeromonas salmonicida subsp. salmonicida epithelial damage.probiotic bacteria increase the host health status and protect mucosal tissue against pathogen-caused damage in mammalian models. using an in vitro (intestinal sac) method this study aimed to address (a) the in vitro ability of lactobacillus delbrueckii subsp. lactis to remain in the gastrointestinal tract of atlantic salmon (salmo salar l.) and (b) its ability to prevent cellular damage caused by successive incubation with aeromonas salmonicida subsp. salmonicida the causative agent of furuncul ...200818054448
antibacterial action of l-amino acid oxidase from the skin mucus of rockfish sebastes schlegelii.l-amino acid oxidase (lao) shows broadly antibacterial activity against gram-positive and gram-negative bacteria by h(2)o(2) generated in the oxidative process of l-amino acids. however, lao (termed ssap) isolated from the rockfish sebastes schlegelii skin mucus acted selectively on gram-negative bacteria. therefore, this study was undertaken to clarify the antibacterial action of ssap as compared with h(2)o(2). ssap inhibited potently the growth of aeromonas salmonicida, photobacterium damselae ...200818055243
structural characterization of the o-chain polysaccharide of aeromonas caviae atcc 15468 lipopolysaccharide.the o-chain polysaccharide produced by a mild acid degradation of aeromonas caviae atcc 15468 lipopolysaccharide was found to be composed of l-rhamnose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose and phosphoglycerol. subsequent methylation and ce-esims analyses and 1d/2d nmr ((1)h, (13)c and (31)p) spectroscopy showed that the o-chain polysaccharide is a high-molecular-mass acidic branched polymer of tetrasaccharide repeating units with a phosphoglycerol substituent having th ...200818068695
occurrence of an sme-2-producing serratia marcescens isolate in canada. 200818083009
the effect of specific mhc class i and class ii combinations on resistance to furunculosis in atlantic salmon (salmo salar).the aim was to evaluate the performance of selected individual major histocompatibility complex (mhc) class i and class ii alleles and combinations of these on disease resistance against furunculosis. the material, consisting of 2,145 atlantic salmon (salmo salar) from seven families, contained four mhc class i (uba) alleles and two mhc class ii alpha (daa) alleles. the included alleles were previously shown to influence resistance towards furunculosis. the fish were challenged with the bacteriu ...200818086261
fluid secretion caused by aerolysin-like hemolysin of aeromonas sobria in the intestines is due to stimulation of production of prostaglandin e2 via cyclooxygenase 2 by intestinal cells.to clarify the mechanisms of diarrheal disease induced by aeromonas sobria, we examined whether prostaglandin e2 (pge2) was involved in the intestinal secretory action of a. sobria hemolysin by use of a mouse intestinal loop model. the amount of pge2 in jejunal fluid and the fluid accumulation ratio were directly related to the dose of hemolysin. the increase over time in the level of pge2 was similar to that of the accumulated fluid. in addition, hemolysin-induced fluid secretion and pge2 synth ...200818086811
antimicrobial activity of an endophytic xylaria sp.yx-28 and identification of its antimicrobial compound 7-amino-4-methylcoumarin.an endophytic xylaria sp., having broad antimicrobial activity, was isolated and characterized from ginkgo biloba l. from the culture extracts of this fungus, a bioactive compound p3 was isolated by bioactivity-guided fractionation and identified as 7-amino-4-methylcoumarin by nuclear magnetic resonance, infrared, and mass spectrometry spectral data. the compound showed strong antibacterial and antifungal activities in vitro against staphylococcus aureus [minimal inhibitory concentrations (mic) ...200818092158
temperate bacteriophage phio18p from an aeromonas media isolate: characterization and complete genome sequence.a group of 74 aeromonas isolates from surface water of three ponds in bielefeld, germany was screened for prophage induction after uv irradiation. the phage phio18p was induced from the aeromonas media isolate o18. phio18p belongs to the myoviridae phage family. the complete nucleotide sequence of the double stranded dna genome of bacteriophage phio18p consists of 33,985 bp. the genome has 5' protruding cohesive ends of 16 bases. on the phio18p genome 46 open reading frames (orfs) were identifie ...200818096197
antibiotic resistance of motile aeromonads in indoor catfish and eel farms in the southern part of the netherlands.the prevalence and degree of antibiotic resistance in catfish and eel farms in the southern part of the netherlands was examined using motile aeromonads as indicator bacteria. a total of 29 water samples were collected, originating from six catfish farms, one catfish hatchery and three eel farms, and were plated on an aeromonas-selective agar with and without antibiotics. from each plate, one colony was screened for presumptive motile aeromonads and tested for antibiotic susceptibility. the prev ...200818160266
fulminant epiglottitis with evolution to necrotizing soft tissue infections and fasciitis due to aeromonas hydrophila. 200818165860
microbiologic study of soft contact lenses after laser subepithelial keratectomy for myopia.to evaluate the extent and agents of bacterial contamination of bandage disposable soft contact lenses after laser subepithelial keratectomy (lasek) and to correlate the findings with clinical data.200818180679
study on the biocompatibility of novel terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate).terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (phbvhhx) containing 5.3 mol % 3-hydroxyvalerate (3-hv) and 10.2 mol % 3-hydroxyhexanoate was obtained via microbial synthesis using recombinant aeromonas hydrophila. for the first time in vitro biocompatibility of the terpolyester was evaluated in comparison with poly(l-lactic acid), poly (3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx). sc ...200818186048
a possible new cause of spa bath folliculitis: aeromonas hydrophila.a 34-year-old man presented with a pubic eruption of 4 weeks duration, affecting both himself and his partner. he had been treated unsuccessfully with intravenous and oral dicloxacillin. the eruption was a severely inflammatory folliculitis, with haemo-serous exudate and marked oedema. a clinical diagnosis of herpes simplex virus folliculitis was disproved when routine bacteriology isolated aeromonas hydrophila and multiple viral polymerase chain reaction studies were negative. histology demonst ...200818186847
aeromonas-acanthamoeba interaction and early shift to a viable but nonculturable state of aeromonas by acanthamoeba.to investigate the hypothesis that amoeba may comprise a significant environmental reservoir for aeromonas, acanthamoeba-aeromonas interaction experiments were performed.200818194253
chemical markers for the evaluation of raw material hygienic quality in egg products.the aim of this research was to study uracil and lactic and acetic acids as chemical markers for hygienic quality evaluation of raw material in liquid pasteurized egg products. uracil, absent in sound whole eggs, was formed in raw and pasteurized egg products as a consequence of high microbial contamination (>10(6) cfu/g) after a sufficient lag time, remaining stable at 4 degrees c but disappearing after 7 days of storage at 25 degrees c. both lactic and acetic acids, starting from initial value ...200818211016
contribution of type iv pili to the virulence of aeromonas salmonicida subsp. salmonicida in atlantic salmon (salmo salar l.).aeromonas salmonicida subsp. salmonicida, a bacterial pathogen of atlantic salmon, has no visible pili, yet its genome contains genes for three type iv pilus systems. one system, tap, is similar to the pseudomonas aeruginosa pil system, and a second, flp, resembles the actinobacillus actinomycetemcomitans flp pilus, while the third has homology to the mannose-sensitive hemagglutinin pilus of vibrio cholerae. the latter system is likely nonfunctional since eight genes, including the gene encoding ...200818212071
pathogenic vibrio, aeromonas and arcobacter spp. associated with copepods in the straits of messina (italy). 200818215401
aeromonas hydrophila ah-3 aext is an adp-ribosylating toxin secreted through the type iii secretion system.we cloned and sequenced an adp-ribosylating toxin (aext) from a mesophilic aeromonas hydrophila strain ah-3 with a type iii secretion system (t3ss). this toxin only showed homology, in genes and proteins, with the first half of a. salmonicida aext. the a. hydrophila aext showed adp-ribosyltransferase activity, translocation through the t3ss system, and this a. hydrophila t3ss system is inducible under calcium-depleted conditions. the a. hydrophila aext mutant showed a slight reduction in their v ...200817689917
detection of the causative agent of furunculusis, aeromonas salmonicida in salmonids of the krka river.in this paper we describe the bacterial community associated with salmonids from the krka river. diversity analysis demonstrated that majority of the recovered bacteria were related to aeromonadaceae group. bacterial analysis also revealed the presence of shigella spp. and pseudomonas fluorescens. isolation of aeromonas salmonicida from trout, presents first isolation of this bacteria croatian rivers.200817624808
virulence and antibiotic susceptibility of aeromonas spp. isolated from drinking water.aeromonas isolates from tap water, mineral water, and artesian well water were investigated for their ability to produce different potential virulence factors or markers such as hemolysins, cytotoxins, phospholipase, dnase, hydrophobicity and their ability to adhere to epithelial cells and to abiotic surfaces. the susceptibility to antibiotics of aeromonas isolates was also examined. majority of the isolates displayed hemolytic activity against sheep erythrocytes, while only 7 of the 23 aeromona ...200817636377
evaluation of adherence, hydrophobicity, aggregation, and biofilm development of flavobacterium johnsoniae-like isolates.flavobacterium spp. isolates have been identified in diverse biofilm structures, but the mechanism of adherence has not been elucidated. the absence of conventional biofilm-associated structures such as fimbriae, pili, and flagella suggest that surface hydrophobicity, and/or autoaggregation and coaggregation may play an important role in adherence and biofilm formation. the biofilm-forming capacity of 29 flavobacterium johnsoniae-like isolates obtained from south african aquaculture systems was ...200817401596
assessment of safety, nutritional, and spoilage characteristics of different lagoon grey mullets (liza ramada, liza aurata, and liza saliens).different lagoon grey mullets such as liza ramada (thinlip mullet), liza aurata (golden grey mullet), and liza saliens (leaping grey mullet) were analyzed for their nutritional, microbiological, and safety parameters. the microbiological values never exceeded the lower limits stipulated by the italian higher institute of health. the pathogenic species frequently associated with seafood (salmonella, listeria monocytogenes, vibrio cholerae, vibrio parahaemolyticus, and aeromonas hydrophila) were n ...200819244917
purification, virulence and characterization of an extracellular peptidase produced by aeromonas salmonicida ssp. masoucida isolated from cultured sea cucumber apostichopus japonicus.strain h1, aeromonas salmonicida ssp. masoucida isolated from an ulcer on the sea cucumber apostichopus japonicus proved to be virulent toward this holothurian at 12 degrees c. extracellular products (ecp) of strain h1 were prepared, and an extracellular peptidase of 42 kda was purified from the ecp using sephadex g-100 gel filtration chromatography and diethylaminoethyl sepharose ion exchange chromatography. stepwise virulence tests on sea cucumbers indicated that the 42 kda peptidase was the v ...200819244974
[taxonomic characteristics and physiological properties of microorganisms from the gut of pike (esox lucius)].the taxonomic composition and distribution of microorganisms differing in the degree of association with the intestinal mucosa of the pike (lucius lucius) has been studied. microorgansism of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae dominate in the gut microflora. numerically prevailing bacterial species are characterized by high proteolytic and amylolytic enzyme activities as well as by high persistence accounted for by antilysozyme and antihistone activities. the result ...200819198074
polyhydroxyalkanoates production from carbohydrates by a genetic recombinant aeromonas sp.to develop an aeromonas strain able to utilize inexpensive carbon sources such as starch for the synthesis of polyhydroxyalkanoates (pha).200819120931
toxin production by aeromonas sobria in natural environments: river water vs. seawater.aeromonas are water-borne pathogens. they are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% nacl). however, the presence of aeromonas in seawater is extremely rare compared with that in river water. in this study, we tested the ability of aeromonas sobria to produce toxins in river water and seawater. first, we cultured a. sobria on skim milk agar plates supplemented with either river water (sarw) or seawater (sasw). the ...200819122681
interaction of aeromonas spp. human isolates with murine macrophages.this study aimed to evaluate phagocytic and bactericidal activity of macrophages originated from murine j774 macrophage cell line against 26 strains of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila isolated from human fecal specimens. the analysis of phagocytic index (pi) indicated these strains were phagocytosed in low numbers, below 1.1 x l0(-3) for 65% strains of a. veronii biotype sobria and a. hydrophila. the highest pi value (0.019) was noted in murine macroph ...200819123303
[selection and kinetic mechanism of psychrotrophs in low temperature wastewater treatment].a study was carried out taking low temperature domestic wastewater as target in laboratory, the composition of bacteria in activated sludge was analyzed and the degradation kinetics of organics was investigated. six preponderant psychrotrophs were selected out, belonging to zoogloea, aeromonas, flavobacterium, micrococcus, bacillus and pseudomonaus, respectively. results show that ttc-dehydrogenase activity of the psychrotroph is 25.44 mg/(l x h) which is 20.5 times more than that of ordinary ac ...200819186826
identification of human enteric pathogens in gull feces at southwestern lake michigan bathing beaches.ring-billed (larus delawarensis ord, 1815) and herring (larus argentatus pontoppidan, 1763) gulls are predominant species of shorebirds in coastal areas. gulls contribute to the fecal indicator burden in beach sands, which, once transported to bathing waters, may result in water quality failures. the importance of these contamination sources must not be overlooked when considering the impact of poor bathing water quality on human health. this study examined the occurrence of human enteric pathog ...200819096455
quantification of hurdles: predicting the combination of effects -- interaction vs. non-interaction.combination of disparate as well as related antimicrobial effects constitutes the concept of hurdle technology. quantification of combined effects, including claims of synergy, can be accomplished using surface response modelling, as is frequently done and reported. the gamma hypothesis, however, states that the relative effects of different antimicrobial factors combine independently. studies performed using time to detection have shown that the gamma hypothesis is an adequate foundation for th ...200818703251
mutation in the s-ribosylhomocysteinase (luxs) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of aeromonas hydrophila.a diarrheal isolate ssu of aeromonas hydrophila produces a cytotoxic enterotoxin (act) with cytotoxic, enterotoxic, and hemolytic activities. our laboratory has characterized from the above aeromonas strain, in addition to act, the type 3- and t6-secretion systems and their effectors, as well as the genes shown to modulate the production of ai-1-like autoinducers, n-acylhomoserine lactones (ahls) involved in quorum sensing (qs). in this study, we demonstrated the presence of an s-ribosylhomocyst ...200818930130
[severe pneumonia caused by aeromonas veronii biovar sobria: a case report and review of the literature].to describe the clinical features of severe pneumonitis with aeromonas veronii biovar sobria.200819080598
in silico identification of putative drug targets from different metabolic pathways of aeromonas hydrophila.aeromonas hydrophila is a major pathogen both of aquatic and terrestrial organisms, including humans. infection with a. hydrophila results in severe economic losses to the aquaculture industry. in humans, aeromonas hydrophila infections are known to cause gastroenteritis and wound infections. investigations for developing a potential vaccine for its control are underway. the availability of the complete sequence information of a. hydrophila strain atcc 7966t genome has made it possible to carry ...200819032165
routine phenotypic identification of bacterial species of the family pasteurellaceae isolated from animals.pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. some species of pasteurellaceae cause severe diseases with high economic losses in commercial animal husbandry and are of great diagnostic concern. because of new data on the phylogeny of pasteurellaceae, their taxonomy has recently been revised profoundly, thus requiring an improved phenotypic differentiation procedure to identify the individual species of ...200818987220
isolation of mannose-binding c-type lectin from sea lamprey (petromyzon marinus) plasma and binding to aeromonas salmonicida.the sea lamprey (petromyzon marinus) is a parasitic cartilaginous fish of the north american great lakes and a predator of many bony fish species of commercial importance to the fishing industry. mannose-binding c-type lectin (mbl) was isolated by mannan-agarose affinity chromatography from sea lamprey plasma. mannose-binding lectin has not before been identified and quantitated in the plasma of this sea lamprey species. the affinity-purified and 2-me reduced lamprey mbl showed two bands of 35kd ...200818937980
multiplex pcr detection of enterotoxin genes in aeromonas spp. from suspect food samples in northern taiwan.aeromonads possess an array of virulence factors and are causative agents of a number of human infections. among them, genes of one cytotoxic (act) and two cytotonic (alt, ast) enterotoxins are implicated in a human diarrheal disease. a rapid, specific, simultaneous detection of these enterotoxin genes in suspected food poisoning samples is not yet reported. hence, a multiplex pcr assay was designed to amplify the cytotoxic (act), heat-labile cytotonic (alt), and heat-stable cytotonic (ast) ente ...200818939759
in vitro and in vivo studies of the use of some medicinal herbals against the pathogen aeromonas hydrophila in goldfish.aeromonas hydrophila is a ubiquitous and opportunistic bacterial pathogen that produces ulcerative dermatitis under stress conditions and inflicts severe losses on global fisheries and fish culture. this study evaluates the antimicrobial potency of aqueous and ethanolic decoction (individual extract) and concoction (mixed extract) of three common medicinal herbs, turmeric curcuma longa, tulsi plant ocimum sanctum, and neem azadirachta indica, against the in vitro growth of a. hydrophila. among t ...200818942593
oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of atlantic salmon.the effect of oxytetracycline (otc) treatment on intestinal bacterial populations in juvenile atlantic salmon salmo salar was evaluated. oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. restriction fragment length polymorphism and sequencing of 16s rdna from isolates were used to analyze the intestinal microbiota before, during, and after otc administration. the microbiota from untreated fish was more diverse, consisting mainly of pseudomonas, acineto ...200818942594
atypical furunculosis vaccines for atlantic cod (gadus morhua); vaccine efficacy and antibody responses.atypical furunculosis caused by atypical aeromonas salmonicida, is an emerging problem in farming of atlantic cod (gadus morhua) in norway, and vaccines are needed. atypical a. salmonicida comprises a heterogeneous group of bacteria differing in surface antigens such as the a-layer protein and lipopolysaccharides (lps). except for one of the experimental oil-adjuvanted whole cell vaccines based on various isolates they all resulted in moderate protection. no clear correlation between vaccine eff ...200818951938
ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract.in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion.200818828791
diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of coriandrum sativum.the total heterotrophic bacteria, actinomycetes and fungus were enumerated from the rhizosphere and non-rhizosphere soil of 50 selected locally available medicinal plants in and around bharathiar university. in all the plants, population of microorganism were higher in the rhizosphere soil than in the non rhizosphere soil. among the microorganisms, bacterial population was higher in number followed by fungus and actinomycetes. of the medicinal plants, the maximum rhizosphere effect was observed ...200818831345
physico-chemical and microbial characteristics of the coral reef environment of the gulf of mannar marine biosphere reserve, india.investigation on physico-chemical parameters and bacteial characteristics of the coral reef environs of the gulf of mannar biosphere reserve was studied. the study found the influence of different physico-chemical parameters on one another and also on the distribution of the total heterotrophic bacteria (thb) in the coral reef areas. nutrients exhibited considerable seasonal and spatial variations with influence on the bacterial population. coral reef areas recorded higher bacterial population d ...200818831378
molecular and functional characterization of a novel cc chemokine in large yellow croaker (pseudosciaena crocea).a full-length cc chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccc2). its open reading frame (orf) is 282 nucleotides (nt), encoding a polypeptide of 93 amino acids (aa). the deduced lyccc2 contains a 20-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cc chemokines (c(34), c(35), c(61) and c(75)). it shares 17.4-40.4% and 10.0-24.5% aa seq ...200818835451
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