Publications

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[detecting pathogenic aeromonas hydrophila in fish by triplex pcr].to develop a rapid pcr method to detect pathogenic aeromonas hydrophila in fish.200818837375
analysis of 16s rrna gene mutations in a subset of aeromonas strains and their impact in species delineation.characterization of 999 aeromonas strains using a published 16s rdna rflp identification method showed that 8.1% of the strains produced unexpected (hereafter called "atypical") restriction patterns, making their identification uncertain. atypical patterns were due to the presence of nucleotide polymorphisms among the rrn operons of the 16s rrna gene (so-called microheterogeneities). double sequencing signals at certain positions revealed the nucleotide composition was responsible for the microh ...200818843597
[the distribution and antimicrobial resistance tendency of pathogens associated with diarrhea in beijing].to monitor the constituents and resistant tendency of bacterial pathogens isolated from diarrheal patients in our hospital form 1994 to 2005 to offer the basis for guiding epidemiologic study, vaccination research and clinical treatment.200818843955
normal intestinal flora of wild nile crocodiles (crocodylus niloticus) in the okavango delta, botswana.bacterial and fungal cultures were performed from cloacal swabs collected from 29 wild nile crocodiles, captured in the okavango delta, botswana. sixteen species of bacteria and 6 fungal species were cultured. individual crocodiles yielded 1-4 bacterial species, and 0-2 fungal species. the most commonly isolated bacteria were microbacterium, enterococcus faecalis, aeromonas hydrophila, and escherichia coli. no salmonellae were cultured. the most commonly occurring fungus was cladosporium. severa ...200818846850
[new selective indicator medium for detection of aeromonas and plesiomonas: unisc agar].we evaluated a new selective indicator medium (unisc agar) for isolation of classical enteropathogens, aeromonas spp and plesiomonas shigelloides. the xylose fermentation capacity is indicated by a yellow color (fermenting agents) or blue (no fermenting agent). this, together with the oxidase test, establishes it as an indicator for detecting aeromonas and plesiomonas shigelloides. its productivity and selectivity, as assessed using the absolute count index and relative count index, indicate it ...200818853024
influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion.the first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. we examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. the bacteria used were 3 strains of aeromonas hydrophila, and a. salmonicida, edwardsiella tarda and yersinia ruckeri. all bacteria adhered to mucus, but at va ...200818924378
clinical significance of spontaneous aeromonas bacterial peritonitis in cirrhotic patients: a matched case-control study.although aeromonas species are known to cause bacteremia in patients with cirrhosis, less is known about spontaneous bacterial peritonitis (sbp) caused by aeromonas species in these patients.200818484880
family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita.seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ...200818486488
protein expression by aeromonas hydrophila during growth in vitro and in vivo.expression of aeromonas hydrophila cellular and extracellular products (ecps) was examined following culture of the bacterium in vitro, in tryptic soy broth (tsb), and in vivo, in dialysis tubing placed within the peritoneal cavity of common carp (cyprinus carpio l.). whole cell (wc), outer membrane proteins (omps) and ecp components of the bacteria were analysed by 1 dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1d sds-page). additionally, 2d sds-page was used to analy ...200818490132
enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing.in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ...200818493013
impaired plasma clottability induction through fibrinogen degradation by asp, a serine protease released from aeromonas sobria.aeromonas sobria infection often advances to sepsis, in which interaction of bacterial components with plasma proteins possibly causes various disorders. this bacterium releases a serine protease (asp), a putative virulence factor, and binds to fibrinogen. to study the asp effect on fibrinogen, we incubated fibrinogen or plasma with asp and investigated their clotting elicited by thrombin, which converts fibrinogen to a fibrin clot. enzymatically active asp retarded plasma clotting in a dose-dep ...200818462393
distribution of small plasmids in aeromonas salmonicida subsp. salmonicida strains isolated from nw spain and portugal: evidence of clonality in strains isolated from turbot, psetta maxima (l.). 200818471103
in vitro activities of tigecycline against clinical isolates of aeromonas, vibrio, and salmonella species in taiwan.all 198 salmonella isolates (58.6% of isolates were resistant to tetracycline), 92 vibrio isolates (4.4% of isolates were resistant to tetracycline), and 200 of 201 aeromonas isolates (39.3% of isolates were resistant to tetracycline; 1 a. caviae isolate had a tigecycline mic of 4 mug/ml) in our study were susceptible to tigecycline, by u. s. food and drug administration criteria for enterobacteriaceae.200818474585
aeromonas salmonicida peritonitis after eating fish in a patient undergoing capd. 200818474929
a micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in atlantic canada.a micro-dilution technique with changes in optical density (od) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 microg otc (oxytetracycline)ml(-1) was applied to determine growth inhibition (gi) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. growth of control cultures (aeromonas salmonicida) was inhibited at all otc concentrations. some feed pellet samples and under-cage sediments from salmon aquac ...200818589456
isolation of putative probionts from cod rearing environment.survival problems are encountered at early stages of intensive fish rearing and antibiotics are widely used to remedy the situation. probiotics may provide a potential alternative method to protect larvae from opportunistic and pathogenic bacteria and promote a balanced environment. this study was designed to search for new probiotics to target this critical period in cod rearing. potential probionts were selected from the natural microbiota of cod aquacultural environment. the selection was bas ...200818599226
microbial challenge promotes the regenerative process of the injured central nervous system of the medicinal leech by inducing the synthesis of antimicrobial peptides in neurons and microglia.following trauma, the cns of the medicinal leech, unlike the mammalian cns, has a strong capacity to regenerate neurites and synaptic connections that restore normal function. in this study, we show that this regenerative process is enhanced by a controlled bacterial infection, suggesting that induction of regeneration of normal cns function may depend critically upon the coinitiation of an immune response. we explore the interaction between the activation of a neuroimmune response and the proce ...200818606660
lung colonization with aeromonas hydrophila in cystic fibrosis believed to have come from a tropical fish tank. 200818607019
aeromonas tecta sp. nov., isolated from clinical and environmental sources.five aeromonas strains, isolated from both clinical and environmental sources and characterized by a polyphasic approach, including phylogenetic analysis derived from gyrb, rpod, and 16s rrna gene sequencing, as well as dna-dna hybridization, extensive biochemical and antibiotic susceptibility tests, were recognized as members of an unknown, or undescribed, aeromonas species. these "aeromonas eucrenophila-like" strains were closely related to the species a. eucrenophila and aeromonas encheleia, ...200818617346
real-time pcr detection of pathogenic microorganisms in roof-harvested rainwater in southeast queensland, australia.in this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of escherichia coli, enterococci, clostridium perfringens, and bacteroides spp. in rainwater obtained from tanks in southeast queensland, australia. samples were also tested using real-time pcr (with sybr green i dye) for the presence of potential pathogenic microorganisms. of the 27 rainwater samples tested, 17 (63%), 21 (78%), 13 (48%), and 24 (89%) were positive for e. coli, en ...200818621865
thrombotic microangiopathy as a complication of medicinal leech therapy.medicinal leeches (hirudo medicinalis) are increasingly used as a useful therapeutic option in various medical and surgical settings. the potential complications associated with this therapy include infections with aeromonas species, bleeding, anemia, and allergic reactions. we describe a patient who developed thrombotic microangiopathy and acute renal failure following leech therapy. it is proposed that the series of nonhuman proteins within leech saliva may be capable of injuring the endotheli ...200818622333
emerging extra-intestinal infections with aeromonas hydrophila in coastal region of southern karnataka.aeromonas species are gram-negative rods usually isolated from the gastrointestinal tract. they have been occasionally reported as a cause of extra-intestinal infections such as cellulitis, cholangitis, necrotizing fascitis, meningitis, bacteremia, or peritonitis in both immunocompetent and immunocompromised patients.200818626167
major histocompatibility complex based resistance to a common bacterial pathogen of amphibians.given their well-developed systems of innate and adaptive immunity, global population declines of amphibians are particularly perplexing. to investigate the role of the major histocompatibility complex (mhc) in conferring pathogen resistance, we challenged xenopus laevis tadpoles bearing different combinations of four mhc haplotypes (f, g, j, and r) with the bacterial pathogen aeromonas hydrophila in two experiments. in the first, we exposed ff, fg, gg, gj, and jj tadpoles, obtained from breedin ...200818629002
therapeutic potential of two probiotics in inflammatory bowel disease as observed in the trinitrobenzene sulfonic acid model of colitis.the pathogenesis of inflammatory bowel disease is thought to be a multifactorial process. one of the leading hypotheses is that an imbalance in normal gut flora induces an excessive immune response and contributes to inflammation in the gastrointestinal tract. administration of probiotic bacteria reduces symptoms in patients suffering from inflammatory bowel diseases, probably via both manipulation of the microflora and stimulation of the intestinal immune system. in the current study the therap ...200818633675
mutational analysis of the zinc- and substrate-binding sites in the cpha metallo-beta-lactamase from aeromonas hydrophila.the subclass b2 cpha (carbapenemase hydrolysing aeromonas) beta-lactamase from aeromonas hydrophila is a zn(2+)-containing enzyme that specifically hydrolyses carbapenems. in an effort to evaluate residues potentially involved in metal binding and/or catalysis (his(118), asp(120), his(196) and his(263)) and in substrate specificity (val(67), thr(157), lys(224) and lys(226)), site-directed mutants of cpha were generated and characterized. our results confirm that the first zinc ion is in interact ...200818498253
development of multiplex pcr assay for simultaneous detection of five bacterial fish pathogens.a multiplex polymerase chain reaction (pcr) method was designed for the simultaneous detection of the five major fish pathogens, aeromonas hydrophila, aeromonas salmonicida subsp. salmonicida, flavobacterium columnare, renibacterium salmoninarum, and yersinia ruckeri. each of the five pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. the detection limits of the multiplex pcr was in the range of 2, 1, 1, 3, and 1cfu for a. hydrophila, a. salmo ...200818499358
plasmid-mediated qnrs2 determinant from a clinical aeromonas veronii isolate. 200818505860
c1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis.except for the complement c1q, the immunological functions of other c1q family members have remained unclear. here we describe zebrafish c1q-like, whose transcription and translation display a uniform distribution in early embryos, and are restricted to mid-hind brain and eye in later embryos. in vitro studies showed that c1q-like could inhibit the apoptosis induced by actd and chx in epc cells, through repressing caspase 3/9 activities. moreover, its physiological roles were studied by morpholi ...200818514183
conformational changes in the metallo-beta-lactamase imis during the catalytic reaction: an epr spectrokinetic study of co(ii)-spin label interactions.metallo-beta-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. in consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. there exists circumstantial evidence that the binding of substrates and inhibitors to metallo-beta-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ...200818528987
identification of heme uptake genes in the fish pathogen aeromonas salmonicida subsp. salmonicida.aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish, can use heme as the sole iron source. we applied the fur titration assay to isolate a cluster including six genes hutazxbcd that showed similarity to heme uptake genes of other gram-negative bacteria, and three genes orf123 of unknown function. the spatial organization of these nine genes, arranged in five transcriptional units, was similar to that of a homologous cluster in a. hydrophila. when a tonb system w ...200818535817
serum crp-like protein profile in common carp cyprinus carpio challenged with aeromonas hydrophila and escherichia coli lipopolysaccharide.the potential of c-reactive protein (crp)-like proteins to be used as a biomarker of health status in cultured carp obtained from various european fish lines has been assessed. varying crp-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. crp-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). serum levels of crp-like protein in carp were observ ...200818538390
antimicrobial peptides derived from hemoglobin are expressed in epithelium of channel catfish (ictalurus punctatus, rafinesque).the beta-chain of the respiratory protein hemoglobin (hbbeta), has recently been identified in novel sites, including mammalian macrophages and alveolar epithelium, as well as in gill microsomes of fish. however, the functional significance of extra-erythrocytically expressed hemoglobin has been unclear. here we show inducible expression and upregulation of antimicrobial peptides (amps) homologous to hbbeta in the gill epithelium of channel catfish (ictalurus punctatus) in response to parasitic ...200818538841
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
removal of nitrogen, phosphorus, and organic pollutants from water using seeding type immobilized microorganisms.to study the possibility of removing nitrogen, phosphorus, and organic pollutants using seeding type immobilized microorganisms.200818548855
[microbiological studies of spiced beef burgers in tripoli city, libyan arab jamahiriya].we investigated the microbiological quality of uncooked and cooked spiced beef burger in restaurants and fast food places in tripoli city and surrounding areas. thus 151 samples (92 cooked and 59 uncooked) were analysed microbiologically. the results showed that all samples were highly contaminated with bacteria; 74.5%, 28.8%, 27.1% and 18.6% of the uncooked samples were contaminated with escherichia coli, aeromonas spp., staphylococcus aureus and e. coli o157:h7 respectively. additionally 11.9% ...200818557465
structural basis for the broad-spectrum inhibition of metallo-beta-lactamases by thiols.the development of broad-spectrum metallo-beta-lactamase (mbl) inhibitors is challenging due to structural diversity and differences in metal utilisation by these enzymes. analysis of structural data, followed by non-denturing mass spectrometric analyses, identified thiols proposed to inhibit representative mbls from all three sub-classes: b1, b2 and b3. solution analyses led to the identification of broad spectrum inhibitors, including potent inhibitors of the cpha mbl (aeromonas hydrophila). s ...200818563261
chemical investigation of finlaysonia obovata: part i--a rare triterpene acid showing antibacterial activity against fish pathogens.the antibacterial screening of extracts of the leaves of finlaysonia obovata with hexane, chloroform and alcohol was carried out against fresh water fish pathogenic bacteria viz., micrococcus sp. (multidrug resistant strain), aeromonas hydrophila, pseudomonas aeruginosa, vibrio alginolyticus, staphylococcus aureus, escherichia coli, edwardsiella tarda by disc-assay method. the hexane and chloroform extracts were found active against four and five pathogens, respectively. the highly active chloro ...200818569719
identification of differentially expressed immune-relevant genes in chinese soft-shelled turtle (trionyx sinensis) infected with aeromonas hydrophila.expressed sequence tag (est) analysis is an efficient tool for gene discovery and profiling gene expression. aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. in order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in chinese soft-shelled turtle (trionyx sinensis) experimentally infected with a. hydrophila by suppressio ...200818572252
toxin-screening and identification of bacteria isolated from highly toxic marine gastropod nassarius semiplicatus.bacteria isolated from a highly toxic sample of gastropod nassarius semiplicatus in lianyungang, jiangsu province in july 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. the toxicity of the gastropod sample was 2 x 10(2)mouse unit (mu) per gram of tissue (wet weight). high concentration of tetrodotoxin (ttx) and its analogues (ttxs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography c ...200818573274
an explanation for the effect of inoculum size on mic and the growth/no growth interface.the inoculum effect (ie) is the phenomenon observed where changes in the inoculum size used in an experiment alters the outcome with respect to, for example, the minimum inhibitory concentration of an antimicrobial or the growth/no growth boundary for a given set of environmental conditions. various hypotheses exist as to the cause of the ie such as population heterogeneity and quorum sensing, as well as the null hypothesis - that it is artefactual. time to detection experiments (ttd) were carri ...200818573556
combined physico-chemical treatments based on enterocin as-48 for inactivation of gram-negative bacteria in soybean sprouts.enterocin as-48 was tested for decontamination of soybean sprouts against gram-negative bacteria. although treatment with bacteriocin alone had no effect on salmonella enterica, a synergistic antimicrobial effect was detected at ph 9.0 and in combination with moderate heat treatment. greatest inactivation was achieved for sprouts heated for 5 min at 65 degrees c in an alkaline (ph 9.0) enterocin as-48 solution of 25 microg/ml. bactericidal activity against s. enterica increased greatly when ente ...200818577412
insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology.wastewater treatment plants (wwtps) are a reservoir for bacteria harbouring antibiotic resistance plasmids. to get a comprehensive overview on the plasmid metagenome of wwtp bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. one run on the gs 20 system yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. the obtained plasmid metagenom ...200818586057
expanded-spectrum beta-lactamase per-1 in an environmental aeromonas media isolate from switzerland. 200818644966
immune response of zebrafish (danio rerio) against a newly isolated bacterial pathogen aeromonas hydrophila.a strain of aeromonas hydrophila associated with unusual mortalities in zebrafish (danio rerio) culture facilities was isolated, identified and characterized. in challenge experiments, adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection with viable bacteria and its extracellular products (ecps) reaching very high mortalities in a few hours. the infection, by the viable bacteria or the ecps, caused cell death in kidney, due to the cytotoxic and haemolytic activities ...200818640853
pseudomonas and aeromonas isolates from domestic reptiles and study of their antimicrobial in vitro sensitivity. 200818683065
necrotizing fasciitis and sepsis caused by aeromonas hydrophila after crush injury of the lower extremity.aeromonas hydrophila is a motile gram-negative bacillus found in water sources that typically causes minor skin infections or gastroenteritis in humans. there are sporadic reports of cases of sepsis or necrotizing fasciitis caused by a. hydrophila but no other cases of severe infection secondary to trauma. the mortality rate of septic shock caused by a. hydrophila approaches 100%.200818687029
characterization of the digestive-tract microbiota of hirudo orientalis, a european medicinal leech.fda-approved, postoperative use of leeches can lead to bacterial infections. in this study, we used culture-dependent and culture-independent approaches to characterize the digestive-tract microbiota of hirudo orientalis. surprisingly, two aeromonas species, a. veronii and a. jandaei, were cultured. uncultured rikenella-like bacteria were most similar to isolates from hirudo verbana.200818689513
[the importance of aeromonads as a human pathogen].the genus aeromonas belongs to the family aeromonadaceae of the order aeromonadales and consists of 14 phenospecies and 17 genomospecies. they are gram-negative, oxidase-positive, facultatively anaerobic and glucose-fermenting rods. members of the mesophilic genus aeromonas spp. are ubiquitous in the aquatic environment and can be isolated from fresh, brackish and marine water. in nutrient-enriched waters they can attain large populations, particularly in the warm seasons at higher temperatures. ...200818696148
monitoring bacterial and archaeal community shifts in a mesophilic anaerobic batch reactor treating a high-strength organic wastewater.shifts in bacterial and archaeal communities, associated with changes in chemical profiles, were investigated in an anaerobic batch reactor treating dairy-processing wastewater prepared with whey permeate powder. the dynamics of bacterial and archaeal populations were monitored by quantitative real-time pcr and showed good agreement with the process data. a rapid increase in bacterial populations and a high rate of substrate fermentation were observed during the initial period. growth and regrow ...200818647356
[application of gyrb in the identification of closely related bacteria-- a review].the single-copied gyrb gene, encoding the subunit b of gyrase and distributing universally in all bacteria, has the average substitution rate of 0.7%-0.8% per million years. it's proved that this region could be used to discriminate and identify the closely related species of different bacteria such as pseudomonoas, bacillus, vibrio, enterobacteriaceae, mycobacteria, aeromonas, lactic acid bacteria et.al. it also can be used of quantitative or restriction fragment analysis of bacteria with the a ...200818652307
necrotizing fasciitis in patients with liver cirrhosis: predominance of monomicrobial gram-negative bacillary infections.necrotizing fasciitis (nf), a rare but severe infection, usually occurs in individuals with underlying chronic illness, but its clinical presentation among cirrhotic patients is infrequently discussed. forty-two cirrhotic patients with 47 episodes of nf between 1995 and 2006 were analyzed. their mean age was 55.6 years, with male preponderance (34 patients, 81%). lower extremities were mainly involved (70%). of 42 episodes with identified pathogens, 41 (97%) were monomicrobial infections and wer ...200818653302
structure of asce and induced burial regions in asce and ascg upon formation of the chaperone needle-subunit complex of type iii secretion system in aeromonas hydrophila.in the type iii secretion system (t3ss) of aeromonas hydrophila, the putative needle complex subunit ascf requires both putative chaperones asce and ascg for formation of a ternary complex to avoid premature assembly. here we report the crystal structure of asce at 2.7 a resolution and the mapping of buried regions of asce, ascg, and ascf in the asceg and ascefg complexes using limited protease digestion. the dimeric asce is comprised of two helix-turn-helix monomers packed in an antiparallel fa ...200818662905
sdia, an n-acylhomoserine lactone receptor, becomes active during the transit of salmonella enterica through the gastrointestinal tract of turtles.luxr-type transcription factors are typically used by bacteria to determine the population density of their own species by detecting n-acylhomoserine lactones (ahls). however, while escherichia and salmonella encode a luxr-type ahl receptor, sdia, they cannot synthesize ahls. in vitro, it is known that sdia can detect ahls produced by other bacterial species.200818665275
use of vitamin c as an immunostimulant. effect on growth, nutritional quality, and immune response of labeo rohita (ham.).teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (aa), an essential micronutrient for fish. the aim of this study was to examine the effect of higher levels of dietary vitamin c on growth, nutritional quality, and immunomodulation in the indian major carp, rohu (labeo rohita). four groups of l. rohita were fed experimental diets containing either no vitamin c (control) or supplemented with vitamin c at 500 mg kg(-1) (exp-1), 1000 mg kg(-1) (exp-2), or 1500 mg kg(-1) (exp-3) ...200818665463
contribution of food deprivation to the immune response in rainbow trout (oncorhynchus mykiss) vaccinated against cryptobia salmositica and aeromonas salmonicida.the aims of the present study were to determine (a) the effectiveness of an attenuated live cryptobia salmositica vaccine; (b) the effects of food deprivation on the immune response and its duration in rainbow trout (oncorhynchus mykiss) immunised with a live c. salmositica vaccine or with a killed aeromonas salmonicida vaccine. the fish were divided into three groups (i, ii and iii; 14 fish per group), those in groups i and ii were under food deprivation (0.40% of body weight), while group iii ...200818669247
the dihydrolipoamide dehydrogenase of aeromonas caviae st exhibits nadh-dependent tellurite reductase activity.potassium tellurite (k(2)teo(3)) is extremely toxic for most forms of life and only a limited number of organisms are naturally resistant to the toxic effects of this compound. crude extracts prepared from the environmental isolate aeromonas caviae st catalize the in vitro reduction of teo32- in a nadh-dependent reaction. upon fractionation by ionic exchange column chromatography three major polypeptides identified as the e1, e2, and e3 components of the pyruvate dehydrogenase (pdh) complex were ...200818675788
polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida.the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ...200818783128
microbial levan in the diet of labeo rohita hamilton juveniles: effect on non-specific immunity and histopathological changes after challenge with aeromonas hydrophila.a 60-day feeding trial was conducted to study the immuno-protective effect of microbial levan on labeo rohita juveniles challenged with aeromonas hydrophila. six purified diets were prepared with different levels of microbial levan: control (no levan), t1 (basal + 0.25%), t2 (basal + 0.50%), t3 (basal + 0.75%), t4 (basal + 1%) and t5 (basal + 1.25%), fed to six groups of fish in triplicate. among the treatment groups the haemoglobin content and total leucocyte count were increased with a dietary ...200818786027
surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006.the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ...200818786028
immune responses and enhanced disease resistance in chinese drum, miichthys miiuy (basilewsky), after oral administration of live or dead cells of clostridium butyrium cb2.clostridium butyrium cb2 isolated from chickens was tested as a potential fish probiotic in the chinese drum, miichthys miiuy. fish were fed live (cb), dead cb2 (d-cb) cells (10(8) cells g(-1)) or spent culture supernatant (scs), for 30 days and challenged with vibrio anguillarum or aeromonas hydrophila. survival was higher in both the cb and the cb-d fed groups, but the scs group was not significantly different from the control. after feeding live or dead cb2 cells, there was increase in phagoc ...200818786030
in vitro evaluation on adherence and antimicrobial properties of a candidate probiotic clostridium butyricum cb2 for farmed fish.to characterize the antimicrobial and adhesion ability of candidate probiotic clostridium butyricum cb2 for farmed fish in vitro.200818795975
identification of the antimicrobial substances produced by solanum palinacanthum (solanaceae).to find out natural antimicrobial agents as alternative in therapeutics and to preserve food, the methanol extract of solanum palinacanthum aerial parts was submitted to purification steps guided by antibacterial and antifungal assays. as a consequence, the flavonoid rutin and 3,5-dicaffeoylquinic acid were isolated by column chromatography and high performance liquid chromatography, and identified by mass and nuclear magnetic resonance spectrometry. minimal inhibitory concentrations (mic) of th ...200818797795
determination of viability of aeromonas hydrophila in increasing concentrations of sodium chloride at different temperatures by flow cytometry and plate count technique.aeromonads in waters and foods can represent a risk to human health. factors such as sodium chloride concentration and temperature can affect growth and viability of several food and water-borne pathogens. the behaviour of an aeromonas hydrophila strain in the presence of 1.7%, 3.4% and 6% nacl concentrations at 24 degrees c and 4 degrees c was studied over a 188 day period. viability and membrane potential were assessed by flow cytometry; growth was evaluated by plate count technique. flow cyto ...200818765166
bacterial communities on electron-beam pt-deposited electrodes in a mediator-less microbial fuel cell.the content of a bacterial consortium found on an electron beam (e-beam) pt-deposited electrode in a mediator-less microbial fuel cell (mfc) using glucose and glutamate as fuel is reported in this paper. the e-beam pt-deposited electrode and electrochemically active bacteria (eab) consortium were developed to improve the mediator-less mfc performance. denaturing gradient gel electrophoresis (dgge), restriction fragment length polymorphism (rflp), and 16s rrna sequencing were used to identify the ...200818767694
effect of bacterial interference on biofilm development by legionella pneumophila.in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ...200818769851
plasmid-mediated quinolone resistance in aeromonas allosaccharophila recovered from a swiss lake.to search for plasmid-mediated qnr genes among waterborne environmental aeromonas spp. recovered from switzerland.200818772162
the genome of aeromonas salmonicida subsp. salmonicida a449: insights into the evolution of a fish pathogen.aeromonas salmonicida subsp. salmonicida is a gram-negative bacterium that is the causative agent of furunculosis, a bacterial septicaemia of salmonid fish. while other species of aeromonas are opportunistic pathogens or are found in commensal or symbiotic relationships with animal hosts, a. salmonicida subsp. salmonicida causes disease in healthy fish. the genome sequence of a. salmonicida was determined to provide a better understanding of the virulence factors used by this pathogen to infect ...200818801193
antibacterial agents and heavy metal resistance in gram-negative bacteria isolated from seawater, shrimp and sediment in iskenderun bay, turkey.the aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from seawater, sediment and shrimps, and to determine if there is a relationship between antibiotic and heavy metal resistance. we undertook studies in 2007 in the industrially polluted iskenderun bay, on the south coast of turkey. the resistance of 236 gram-negative bacterial isolates (49 from seawater, 90 from sediment and 97 from shrimp) to ...200818804847
production of c5a by asp, a serine protease released from aeromonas sobria.aeromonas sobria causes pus and edema at sites of infection. however, the mechanisms underlying these effects have not been elucidated. c5a, the amino-terminal fragment of the complement 5th component (c5), mimics these events. to investigate the involvement of c5a in the pathophysiology of a. sobria infection, we examined release of c5a from human c5 by a serine protease (asp), a putative virulence factor secreted by this bacterium. c5 incubated with enzymatically active asp induced neutrophil ...200818714034
molecular diversity of class 1 integrons in human isolates of aeromonas spp. from southern taiwan.this work studies antimicrobial resistance and class 1 integrons of aeromonas spp. in human isolates from southern taiwan. pcr amplification and dna sequence analyses were performed to characterize the gene cassette regions of the class 1 integron in 204 isolates of aeromonas hydrophila, 36 isolates of a. sobria, 23 isolates of a. veronii, and 4 isolates of a. caviae. by using southern hybridization with an inti1 probe to determine the presence of class 1 integrons in the 9 isolates of aeromonas ...200818806339
incidence of bacterial enteropathogens among hospitalized diarrhea patients from orissa, india.bacteriological analysis of 1,551 stool/rectal swabs from all age groups of diarrhea patients of different hospitals of orissa from january 2004 to december 2006 was carried out using standard procedures. among all enteropathogens isolated in 886 culture-positive samples, escherichia coli constituted 75.5%, including 13.2% pathogenic e. coli; vibrio cholerae o1 constituted 17.3%; v. cholerae o139, 1%; shigella spp., 4.5% (shigella flexneri type 6, 2.9%, s. dysenteriae type i, 0.7%, s. sonnei, 0. ...200818806340
[ants in a hospital environment and its importance as vector of bacteria].the external ant community of hospital municipal de morrinhos, in morrinhos, goiás state, was characterized by the low rates of richness, diversity, dominance and equity of species abundance. pheidole sp.1, a polygynic species was numerically dominant in this environment, although it coexists with potentially competitive species. this ant species prevailed within all hospital departments and its space-time distribution was a little aggregated (variance/mean ratio = 1.102, chi2 = 29.38, p < 0.01) ...200818813751
enzymatic characterization of vibrio alginolyticus strains isolated from bivalves harvested at venice lagoon (italy) and guanabara bay (brazil).the aquatic ecosystem is the natural habitat of microorganisms including vibrio and aeromonas genus which are pathogenic to human and animals. in the present investigation the frequency of these bacteria and the enzymatic characteristics of 34 vibrio alginolyticus strains isolated from bivalves harvested in venice lagoon (italy) and guanabara bay (brazil) were carried out from november 2003 to february 2004. the mussels' samples were submitted to enrichment in alkaline peptone water (apw) added ...200818813756
concurrent infection with ranavirus, batrachochytrium dendrobatidis, and aeromonas in a captive anuran colony.four species (dendrobates auratus, phyllobates terribilis, pyxicephalus adspersus, and rhacophorus dennysi) of captive anurans with a clinical history of lethargy and inappetence were found dead and were submitted for necropsy. gross lesions included irregular patches of sloughed skin and rare dermal ulcerations. histologic findings included epidermal proliferation that was most pronounced on the digits and that included intracytoplasmic chytrid organisms. bacteria were often associated with the ...200818817009
expressed sequence tags (ests) from immune tissues of turbot (scophthalmus maximus) challenged with pathogens.the turbot (scophthalmus maximus; scophthalmidae; pleuronectiformes) is a flatfish species of great relevance for marine aquaculture in europe. in contrast to other cultured flatfish, very few genomic resources are available in this species. aeromonas salmonicida and philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in ...200818817567
[preliminary study of the environmental effect on aeromonas hydrophila's survival].a survival of a. hydrophila b3 has been conducted in different conditions (mineral water, seawater exposed or not to the sunlight). also, unculturable forms have been detected by using epifluorescence microscopy. thus, different kinds of microcosms were prepared using filtered and autoclaved marine water or mineral water, inoculated by a. hydrophila b3 and maintained or not in room light. further, we tested the survival of a. hydrophila b3 incubated in seawater and exposed to sunlight. our resul ...200819469413
bacterial indicators of pollution of the douala lagoon, cameroon: public health implications.indiscriminate disposal of untreated wastes which are often heavily laden with sewage microorganisms some of which are pathogenic to humans into aquatic environments near cities could serve as potential dangers to human health.200819357756
aeromonas caviae septicemia in immunocompetent gastrointestinal carriers.aeromonas caviae strains have been isolated from blood and stool cultures of three immunocompetent patients, residents of northern india, who presented with community acquired septicemia without any recent history of diarrhea. cell culture infectivity test performed on hep-2 cells have shown substantial degree of invasiveness in the isolated strains. this case unleashes a possibility of asymptomatic gastrointestinal carriage of such strains of a. caviae in a very large population of india, as se ...200819287850
isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ...200819216104
potential of psidium guajava supplemented fish diets in controlling aeromonas hydrophila infection in tilapia (oreochromis niloticus).fourteen herbs were extracted in water, 95% ethanol, and ether, and tested in vitro for antimicrobial activities against aeromonas hydrophila, a fish pathogen causing motile aeromonas septicemia. using swab paper disc assays and minimal inhibitory concentration (mic) determinations, we noted that the ethanol extract of psidium guajava leaf exhibited the strongest antimicrobial activity. the extract allowed growth-inhibited a. hydrophila cells to regrow in fresh bhi broth indicating a bacteriosta ...200819111636
characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine.to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine.200819552776
biodegradation of carbendazim by epiphytic and neustonic bacteria of eutrophic chełmzyńskie lake.the paper presents a study on biodegradation of carbendazim (1 mg/l) by homogeneous cultures of epiphytic (n = 25) and neustonic (n = 25) bacteria and heterogeneous (n = 1) cultures containing a mixture of 25 bacterial strains isolated from epidermis of the common reed (phragmites australis, (cav.) trin. ex steud.) and surface microlayer (sm approximately 250 microm) of eutrophic lake chełmzyńskie. results indicate that epiphytic bacteria are characterized by higher average capacity to decompose ...200819004243
aeromonas-associated infections in developing countries.although their role in gastroenteritis is controversial, aeromonas species are recognized as etiological agents of a wide spectrum of diseases in man and animals. in developing countries, potentially pathogenic aeromonas sp. are very common in drinking water and in different types of foods, particularly seafood. several food-borne and water-borne outbreaks as well nosocomial outbreaks associated with aeromonads have been reported. significant association of aeromonas sp. with diarrhoea in childr ...200819738331
contamination of community water sources by potentially pathogenic vibrios following sea water inundation.potentially pathogenic members of the vibrionaceae family including vibrio cholerae and vibrio parahemolyticus were isolated from domestic sources of drinking water in coastal villages following sea water inundation during the tsunami in southern india. phenotypic and genotypic studies were done to confirm the identity and detection of toxins. vibrio-gyr (gyrase b gene) was detected in all sixteen vibrio isolates. toxin regulating genes i.e.: ctx gene, tdh gene, and trh gene, however were not de ...200718697592
virulence and resistance markers in clinical and environmental aeromonas strains isolated in romania.the virulence and resistance (r) features of 37 aeromonas strains from diarrheal cases and 150 from the aquatic environment (isolated during cold and warm season) were tested at different incubation temperatures (4 degrees c, 28 degrees c and 37 degrees c). when incubated at 4 degrees c temperature, the aeromonasstrains isolated during the cold season expressed the highest number of virulence factors by comparison with the strains isolated during warm season and from diarrhoeal cases, the virule ...200718928066
the role of aeromonas in diarrhea: a review.the evidence for an enteropathogenic role of aeromonas spp. is still controversial. this review examines various parameters related to a causative role of aeromonas and concludes that infraspecific subsets of strains with a particular array of enterotoxin genes are potential enteropathogens. the consequences for stool bacteriology are discussed.200717401708
metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake.in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ...200717406774
the recently proposed species aeromonas sharmana sp. nov., isolate gptsa-6t, is not a member of the genus aeromonas.a new species of the genus aeromonas, aeromonas sharmana sp. nov., was recently described on the basis of a single isolate, strain gptsa-6t, obtained from a warm spring in india. the description of this new species included biochemical characterization, antibiotic susceptibility, cellular fatty-acid profiles, and 16s rrna gene sequencing, but not dnadna hybridization data. in the present article, phylogenetic analysis (branch distances in the tree and nucleotide signatures) of the 16s rrna of is ...200717407062
quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in aeromonas hydrophila ah-1n.aeromonas hydrophila ah-1n shows biphasic growth in glucose containing broth at 30 degrees c. the first phase is characterized by strong acidification, while the second phase is accompanied by an increase in medium ph. disruption of ahl production by knockout of ahyi did not affect the first growth phase, but resulted in further acidification which completely blocked the second growth phase and even caused cell death. the block could be relieved by addition of 5 microm (or less) synthetic n-buta ...200717408926
immunomodulating effect of inositol hexaphosphate against aeromonas hydrophila-endotoxin.the present study was carried out to evaluate the effect of inositol hexaphosphate (ip6) administration on endotoxemia as an example of the systemic inflammatory response. mice were divided into three groups as follows: first group, remained as a naive group injected intraperitoneally (i.p.) with pbs (ph 7.4; 0.2 ml/mice) at intervals parallel to the treated groups. the second group was injected i.p. with the lipopolysaccharide (lps) of aeromonas hydrophila once a week for four weeks at a dose o ...200717412285
rotavirus disease in young children from hanoi, vietnam.pathogen prevalences and antimicrobial susceptibilities are essential for the rational development of preventive strategies for diarrheal diseases, but little recent information from vietnam is available. we prospectively studied the prevalence of enteric pathogens in children less than 5 years of age with acute diarrhea and in nondiarrhea controls in a city hospital in hanoi, vietnam for 1 year.200717414396
[biodegradation of estrogens in stream water].estrogens of human and animal origin that reach the aquatic environment may enter human or animal organism and act as endocrine disruptors.to investigate the persistency of estrogens in laboratory experiments, estrone respectively 17beta-estradiol were added to stream water sampled from river spree in berlin. the concentration of estrone and 17beta-estradiol was quantified using enzyme-immuno-assay. the estrone concentration decreased to less than 5 % of the starting concentration at storage tem ...200717416137
shape and subunit organisation of the dna methyltransferase m.ahdi by small-angle neutron scattering.type i restriction-modification (r-m) systems encode multisubunit/multidomain enzymes. two genes (m and s) are required to form the methyltransferase (mtase) that methylates a specific base within the recognition sequence and protects dna from cleavage by the endonuclease. the dna methyltransferase m.ahdi is a 170 kda tetramer with the stoichiometry m(2)s(2) and has properties typical of a type i mtase. the m.ahdi enzyme has been prepared with deuterated s subunits, to allow contrast variation u ...200717418232
ulinastatin alone does not reduce caspase 3-mediated apoptosis in protease-positive aeromonas hydrophilia-induced sepsis.to evaluate the effect of ulinastatin, a protease inhibitor, on survival and apoptosis in protease-positive aeromonas hydrophilia (ppah)-induced sepsis.200717339152
infection risk related to the use of medicinal leeches.to assess the incidence of postoperative wound infections related to treatment with medicinal leeches at ghent university hospital.200717353971
vaccination against atypical furunculosis and winter ulcer disease of fish.atypical furunculosis is a problem in farming of salmonids and various other fish species caused by a heterogeneous group of atypical aeromonas salmonicida strains. winter ulcer is a disease of salmonids and cod caused by moritella viscosa, but a number of fish species are susceptible to the infection. vaccines are available against atypical furunculosis of salmonids, but their efficacy is dependent on the characteristics of the infective strain. vaccines for non-salmonid fish are currently not ...200717367897
global transcriptional responses of wild-type aeromonas hydrophila and its virulence-deficient mutant in a murine model of infection.we previously generated a double knockout mutant (act/aopb) of a diarrheal isolate ssu of a. hydrophila, in which the genes encoding aeromonas outer membrane protein b (aopb), a structural component of the type iii secretion system (t3ss), and a type ii (t2)-secreted cytotoxic enterotoxin gene (act) were deleted. this mutant exhibited minimal virulence in mice, compared to animals infected with wild-type (wt) a. hydrophila. based on microarray analyses, wt a. hydrophila altered the expression of ...200717368824
effects of polyherbal formulation 'immuplus' on immunity and disease resistance of indian major carp, labeo rohita at different stages of growth.a series of experiments were performed to determine the impact of polyherbal immunomodulatory formulation 'immuplus' (aquaimmu) on growth, immunity and disease resistance of rohu (labeo rohita), one of the indian major carp at different stages of growth. rohu larvae were fed on plankton, immuplus-mixed compound feed, and plankton plus immuplus-mixed compound feed (immuplus added at three dose levels of 0.25, 0.50, and 0.75 g/kg feed) from 4th day of hatching to 14th day. immuplus-mixed diets enh ...200717373376
quantitative analysis of organ tissue damage after septic shock.the objective of this study was to quantify end-organ damage caused by bacteremic sepsis. twelve adult swine were divided into two groups. the anesthesia control group (n = 6) received general anesthesia for 4 hours. the septic shock group (n = 6) received an infusion of aeromonas hydrophila under general anesthesia for 4 hours. swine were sacrificed at the end of the 4-hour procedure. tissues from lungs, kidneys, livers, and hearts were stained with hematoxylin and eosin. images of tissues were ...200717375779
non-structural flagella genes affecting both polar and lateral flagella-mediated motility in aeromonas hydrophila.an aeromonas hydrophila ah-3 minitn5 mutant unable to produce polar and lateral flagella was isolated, in which the transposon was inserted into a gene whose encoded protein was an orthologue of the campylobacter jejuni motility accessory factor (maf) protein. in addition to this gene, several other related genes were found in this cluster that was adjacent to the region 2 genes of the polar flagellum. mutation of the a. hydrophila ah-3 maf-2, neub-like, flmd or neua-like genes resulted in non-m ...200717379726
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