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incidence of class 1 integron and other antibiotic resistance determinants in aeromonas spp. from rainbow trout farms in australia.there is limited information on antibiotic resistance determinants present in bacteria of aquaculture origin in australia. the presence of integron and other resistance determinants was investigated in 90 aeromonas isolates derived from nine freshwater trout farms in victoria (australia). polymerase chain reaction was carried out for the detection of integrase genes int1, int2 and int3, gene cassette array, integron-associated aada, sul1 and qac1 genes, streptomycin resistance genes stra-strb, ß ...201121762170
a unique epidermal mucus lectin identified from catfish (silurus asotus): first evidence of intelectin in fish skin slime.the present study reports a new type of skin mucus lectin found in catfish silurus asotus. the lectin exhibited calcium-dependent mannose-binding activity. when mannose eluate from chromatography with mannose-conjugated agarose was analyzed by sds-page, the lectin appeared as a single 35-kda band. gel filtration showed that the lectin forms monomers and dimers. a 1216-bp cdna sequence obtained by race-pcr from the skin encoded a 308 amino acid secretory protein with homology to mammalian and fis ...201121757471
three goose-type lysozymes in the gastropod oncomelania hupensis: cdna sequences and lytic activity of recombinant proteins.three goose-type (g-type) lysozymes, designated as ohlysg1, ohlysg2 and ohlysg3 were identified from expressed sequence tags (ests) of a gastropod oncomelania hupensis, the intermediate host of schistosoma japonicum. the full cdna sequences of ohlysg1, ohlysg2 and ohlysg3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the n-terminus, respectively. the three g-type lysozymes shared conserv ...201121756934
erratum: purification of a toxic cysteine protease produced by pathogenic aeromonas hydrophila isolated from rainbow trout.an extracellular lethal toxin produced by aeromonas hydrophila strain rt860715k originally isolated from diseased rainbow trout (oncorhynchus mykiss) was purified by using fast protein liquid chromatography system with hydrophobic interaction chromatography and anion exchange columns. the toxin was a cysteine protease, inhibited by l-cysteine, iodoacetic acid, n-ethylamleimide, p-chloromercuibenzene-sulfonic acid and n-α-p-tosyl-1-lysine-chloromethyl ketone (tlck), and showed maximal activity at ...201121630504
influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (sparus aurata) and intestinal contents in goldfish (carassius auratus).fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. the objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (sparus aurata) and in goldfish (carassius auratus). faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. vib ...201121692817
identification and expression profiles of multiple genes in nile tilapia in response to bacterial infections.to understand the molecular mechanisms involved in response of nile tilapia (oreochromis niloticus) to bacterial infection, suppression subtractive cdna hybridization technique was used to identify upregulated genes in the posterior kidney of nile tilapia at 6h post infection with aeromonas hydrophila. a total of 31 unique expressed sequence tags (ests) were identified from 192 clones of the subtractive cdna library. quantitative pcr revealed that nine of the 31 ests were significantly (p<0.05) ...201121840065
bacterial symbiont and salivary peptide evolution in the context of leech phylogeny.summarythe evolutionary history of leeches is employed as a general framework for understanding more than merely the systematics of this charismatic group of annelid worms, and serves as a basis for understanding blood-feeding related correlates ranging from the specifics of gut-associated bacterial symbionts to salivary anticoagulant peptides. a variety of medicinal leech families were examined for intraluminal crop bacterial symbionts. species of aeromonas and bacteroidetes were characterized ...201121729354
bacterial infection of mudfish clarias gariepinus (siluriformes: clariidae) fingerlings in tropical nursery ponds.bacterial infection among the most common cultured mudfish clarias gariepinus in africa, has become a cause of concern, because it constitutes the largest economic loss in fish farms. in order to provide useful biological data of the pathogens for good management practices, samples were collected monthly between january 2008 and december 2009 in three monoculture nursery ponds, located in three different positions: upriver (a, grassland), mid-river (b, mixed forest and grassland) and downriver ( ...201121717856
lactobacillus plantarum (vr1) isolated from an ayurvedic medicine (kutajarista) ameliorates in vitro cellular damage caused by aeromonas veronii.abstract:201121707995
a specific inorganic triphosphatase from nitrosomonas europaea: structure and catalytic mechanism.the cyth superfamily of proteins is named after its two founding members, the cyab adenylyl cyclase from aeromonas hydrophila and the human 25-kda thiamine triphosphatase. because these proteins often form a closed +¦-barrel, they are also referred to as "triphosphate tunnel metalloenzymes" (ttm). functionally, they are characterized by their ability to bind triphosphorylated substrates and divalent metal ions. these proteins exist in most organisms and catalyze different reactions, depending on ...201121840996
prevalence, characterization, and antimicrobial resistance of aeromonas strains from various retail food products in mumbai, india.ôçé a total of 154 food samples (chicken, fish, and ready-to-eat sprouts) from various retail outlets in mumbai, india, were analyzed for the presence of aeromonas spp. over a period of 2 y (january 2006 to march 2008). twenty-two aeromonas isolates belonging to 7 different species were isolated from 18 (11.7%) food samples. the highest percentages of isolation were from chicken (28.6%) followed by fish (20%) and sprout (2.5%) samples. aeromonas caviae, a. veronii bv. sobria, and a. salmonicida ...201121824136
aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type iii secretion system.in the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type iii secretion system (ttss) in 64 aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. we observed that contact of the bacteria with host epithelial cells is a prerequisite for their cytotoxicity at 3 h incubation. cell-contact cytotoxic activity of the strains was strongly associated with the presence of the ttss. culture supernatants of the strains induced low ...201121809027
the microbiological profile and presence of bloodstream infection influence mortality rates in necrotizing fasciitis.abstract: introduction: necrotizing fasciitis (nf) is a life threatening infectious disease with a high mortality rate. we carried out a microbiological characterization of the causative pathogens. we investigated the correlation of mortality in nf with bloodstream infection and with the presence of co-morbidities. methods: in this retrospective study, we analyzed 323 patients who presented with necrotizing fasciitis at two different institutions. bloodstream infection (bsi) was defined as a pos ...201121693053
effects of a prebiotic, immunogen(-«) , on feed utilization, body composition, immunity and resistance to aeromonas hydrophila infection in the common carp cyprinus carpio (linnaeus) fingerlings.this study evaluated the effects of a commercial prebiotic, immunogen, on feed utilization, growth rate, immunity promotion and carcass composition of the common carp cyprinus carpio fingerlings. the fingerlings were adopted for 2ôçâweeks and then reared in triplicate groups in 250-l tanks (nôçâ=ôçâ15 per tank with average initial weights of 11.12ôçâ-¦ôçâ0.55ôçâg). the fish fed on five isonitrogenous and isoenergetic experimental diets containing different levels of immunogen (0, 0.5, 1, 1.5 and ...201121707781
aeromonas hydrophila moty is essential for polar flagellum function, requires coordinate expression of motx and pom proteins.by the analysis of aeromonas hydrophila atcc7966t genome we identified a. hydrophila ah-3 moty. a. hydrophila moty, like motx, is essential for the polar flagellum function energized by an electrochemical potential of na+ as coupling ion, but is not involved in lateral flagella function energized by proton motive force. thus, a. hydrophila polar flagellum stator is a complex integrated by two essential proteins: motx and moty, which interact with one of two redundant pairs of proteins: pomab and ...201121737499
differential immune responses of the green neon shrimp (neocaridina denticulate) to dipropyl phthalate.this study set out to understand the sublethal effect of xenobiotic phthalate esters (paes) on the relationship between the susceptibility of shrimp to bacterial infection and the immune response of the shrimp. neocaridina denticulate were exposed to different concentrations of the pae dipropyl phthalate (dprp), and mortality and six immune parameters were measured on days 1, 3, 5, and 10 after exposure. on days 1 and 3 after exposure, shrimp exposed to 0, 1, 5, 10, and 50 mg/l of dprp and chall ...201121740974
distribution and expression analysis of transcription factors in tissues and progenitor cell populations of the goldfish (carassius auratus l.) in response to growth factors and pathogens.we report on the mrna levels of a panel of transcription factors in the kidney and spleen tissues, and in the cell populations from the blood, the spleen, and in the sorted kidney progenitor cells. the mrna levels of cebpα, cjun, cmyb, egr1, gata1, gata2, gata3, lmo2, mafb, pax5, pu.1 and runx1 were assessed in healthy goldfish as well as in fish challenged with two different pathogens, aeromonas salmonicida a449 or trypanosoma carassii. spleen tissue from healthy goldfish showed higher expressi ...201121474183
application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents.a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ...201121419572
leech-transmitted ciprofloxacin-resistant aeromonas hydrophila. 201121339408
purification and characterization of enterocin mc13 produced by a potential aquaculture probiont enterococcus faecium mc13 isolated from the gut of mugil cephalus.a bacteriocin producer strain mc13 was isolated from the gut of mugil cephalus (grey mullet) and identified as enterococcus faecium. the bacteriocin of e. faecium mc13 was purified to homogeneity, as confirmed by tricine sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page). reverse-phase high-performance liquid chromatography (hplc) analysis showed a single active fraction eluted at 26 min, and matrix-assisted laser desorption ionization time of flight (maldi-tof) mass spectro ...201122112158
Acanthamoeba polyphaga, a potential environmental vector for the transmission of food-borne and opportunistic pathogens.The endosymbiotic relationship could represent for many bacteria an important condition favouring their spread in the environment and in foods. For this purpose we studied the behaviour of some food-borne and opportunistic pathogens (Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, Yersinia enterocolitica) when internalized in Acanthamoeba polyphaga. Our results confirm the capability of the bacteria tested to gr ...201121953544
development and efficacy of novobiocin and rifampicin-resistant aeromonas hydrophila as novel vaccines in channel catfish and nile tilapia.three attenuated aeromonas hydrophila vaccines were developed from the virulent 2009 west alabama isolates through selection for resistance to both novobiocin and rifampicin. when channel catfish (ictalurus punctatus) were ip injected with 4×105 colony-forming unit (cfu) of the mutants, no fish died. however, when the same age and size matched channel catfish were ip injected with similar amount of their virulent parents, 80-100% fish died. similarly, when nile tilapia (oreochromis niloticus) we ...201121872628
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).201121936667
metabolic engineering of aeromonas hydrophila 4ak4 for production of copolymers of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoate.a mutant termed aeromonas hydrophila aklf was constructed by deleting acetic acid pathway related genes pta and acka in a. hydrophila 4ak4. accumulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) in a. hydrophila aklf was increased by 47% from 2.11 to 3.10g/l associated with a reduction on acetic acid formation compared with a. hydrophila 4ak4 when lauric acid was used as carbon resource. a. hydrophila aklf harboring pvgab encoding vitreoscilla hemoglobin, +¦-ketothiolase and acet ...201121680176
Bacteremia due to extended-spectrum-ß-lactamase-producing Aeromonas spp. at a medical center in Southern Taiwan.Although extended-spectrum-ß-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combinat ...201121968366
Characterization of anticancer, antimicrobial, antioxidant properties and chemical compositions of Peperomia pellucida leaf extract.Peperomia pellucida leaf extract was characterized for its anticancer, antimicrobial, antioxidant activities, and chemical compositions. Anticancer activity of P. pellucida leaf extract was determined through Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF-7) cell line and the antimicrobial property of the plant extract was revealed by using two-fold broth micro-dilution method against 10 bacterial isolates. Antioxidant activity of the plant extract was then charact ...201122071643
validation of the h2s method to detect bacteria of fecal origin by cultured and molecular methods.using biochemical and molecular methods, this research determined whether or not the h(2)s test did correctly identify sewage-contaminated waters by being the first to use culturing and molecular methods to identify the types and numbers of fecal indicator organisms, pathogens, and other microbes present in sewage samples with positive h(2)s test results. for the culture-based method, samples were analyzed for the presence of fecal bacteria by spread plating the sewage sample onto differential a ...201122038242
complete genomic sequence of a t4-like bacteriophage, phias4, infecting aeromonas salmonicida subsp. salmonicida.a newly identified virulent phage (named phias4) infecting aeromonas salmonicida subsp. salmonicida was isolated from river water in korea. morphological analysis of phias4 by transmission electron microscopy revealed that it belonged to the family myoviridae. the genome of phias4 comprised a linear double-stranded dna of 163,875 bp with a g + c content of 41.3%, and genomic analysis revealed 271 putative orfs, 67 putative promoters, 25 putative terminator regions, and 16 trna-encoding genes. mo ...201122116495
[in process citation]. 201121720695
effect of dietary arginine on growth, intestinal enzyme activities and gene expression in muscle, hepatopancreas and intestine of juvenile jian carp (cyprinus carpio var. jian).the present study was conducted to test the hypothesis that dietary arginine promotes digestion and absorption capacity, and, thus, enhances fish growth. this improvement might be related to the target of rapamycin (tor) and eif4e-binding protein (4e-bp). a total of 1200 juvenile jian carp, cyprinus carpio var. jian, with an average initial weight of 6·33 (se 0·03) g, were fed with diets containing graded concentrations of arginine, namely, 9·8 (control), 12·7, 16·1, 18·5, 21·9 and 24·5 g argini ...201122013925
Screening microorganisms for insulin binding reveals binding by Burkholderia multivorans and Burkholderia cenocepacia and novel attachment of insulin to Aeromonas salmonicida via the A-layer.Exposure to microorganisms is considered an environmental factor which can contribute to Type 1 diabetes. Insulin binding proteins on microorganisms may induce production of antibodies which can react with the human insulin receptor (HIR) with possible consequences in developing a diabetic autoimmune response against HIR and insulin. The interaction of insulin with microorganisms was studied by screening forty-five microbial species for their ability to bind insulin. Binding assays were performe ...201122171975
adsorptive removal of silver nanoparticles (snps) from aqueous solution by aeromonas punctata and its adsorption isotherm and kinetics.silver nanoparticles (snps) are being increasingly used in many consumer products and industrial application. the release of snps to the environment is a major concern. here we have studied the adsorptive removal of snps by a snp resistant bacterial species aeromonas punctata, isolated from the sewage environment. the influence of zeta potential on adsorption was investigated at acidic, neutral and alkaline ph and with varying salt (nacl) concentrations. the rate of adsorption and removal of snp ...201122178439
high prevalence of qnr and aac(6')-ib-cr genes in both water-borne environmental bacteria and clinical isolates of citrobacter freundii in china.we investigated the prevalence of qnr and aac(6')-ib-cr genes in water-borne environmental bacteria and in clinical isolates of enterobacteriaceae, as well as the subtypes of qnr. environmental bacteria were isolated from surface water samples obtained from 10 different locations in hangzhou city, and clinical isolates of citrobacter freundii were isolated from several hospitals in four cities in china. qnra, qnrb, qnrs, and aac(6')-ib-cr genes were screened using pcr, and the genotypes were ana ...201122167061
antimicrobial susceptibility of aeromonas spp. isolated from clinical and environmental sources to 26 antimicrobial agents.we determined the susceptibility of 144 clinical and 49 environmental aeromonas strains representing 10 different species to 26 antimicrobial agents by the agar dilution method. no single species had a predominantly non-susceptible phenotype. a multi-non-susceptible pattern was observed in three (2.1%) clinical strains and two (4.0%) strains recovered from diseased fish. common clinical strains were more resistant than the corresponding environmental isolates suggesting that resistance mechanism ...201122123695
preparation and evaluation of oleoyl-carboxymethy-chitosan (ocmcs) nanoparticles as oral protein carriers.oleoyl-carboxymethy chitosan (ocmcs) nanoparticles based on chitosan with different molecular weights (50, 170 and 820 kda) were prepared by self-assembled method. the nanoparticles had spherical shape, positive surface charges and the mean diameters were 157.4, 274.1 and 396.7 nm, respectively. fitc-labeled ocmcs nanoparticles were internalized via the intestinal mucosa and observed in liver, spleen, intestine and heart following oral deliverance to carps (cyprinus carpio). extracellular produc ...201122139537
Occurrence of enterotoxigenic Aeromonas species in foods of animal origin in North East India.The aim of the present study was to isolate and identify Aeromonas (A.) species and assess their toxin producing ability in foods of animal origin from North East India.201121845798
Bacteriophages with potential for inactivation of fish pathogenic bacteria: survival, host specificity and effect on bacterial community structure.Phage therapy may represent a viable alternative to antibiotics to inactivate fish pathogenic bacteria. Its use, however, requires the awareness of novel kinetics phenomena not applied to conventional drug treatments. The main objective of this work was to isolate bacteriophages with potential to inactivate fish pathogenic bacteria, without major effects on the structure of natural bacterial communities of aquaculture waters. The survival was determined in marine water, through quantification by ...201122163184
clinical observations of black disease in fairy shrimps, streptocephalus sirindhornae and branchinella thailandensis, from thailand and pathogen verification.in this study, black disease infecting fairy shrimps, streptocephalus sirindhornae sanoamuang, murugan, weekers & dumont, and branchinella thailandensis sanoamuang, saengphan & murugan, in thailand, was investigated. the typical signs of the disease are the appearance of black spots on the cuticle, located mainly on the dorsal side and thoracopods. a number of rod-shaped bacteria aggregated in the black spots and were visualized by scanning electron microscopy. the histopathological results show ...201122074019
Identification of differentially expressed protective genes in liver of two rainbow trout strains.Since 1975, the rainbow trout strain BORN (Germany) has been bred in brackish water from a coastal form imported from Denmark. Accompanying phenotypic monitoring of the adapted BORN trout until now revealed that this selection strain manifested a generally elevated resistance towards high stress and pathogenic challenge including lower susceptibility towards Aeromonas salmonicida infections in comparison to other trout strains in local aqua farms. We focus on the elucidation of both, genetic bac ...201122196148
the effect of metals on condition and pathologies of european eel (anguilla anguilla): in situ and laboratory experiments.forty-nine wild eels (anguilla anguilla) caught in the albufera lake (spain), measuring 24.0-75.0cm in length and 25.0-637.7g in weight, were examined for metals (cd, co, cr, cu, fe, hg, mn, pb, se and zn), condition (ci and hsi indices), as well as for diseases (anguillicola infestation; bacterial infections). total metal load significantly increased in eel liver tissue parallel to total length and body weight (log), while silvering females (w(b)>200g; l≥500mm) exhibited the highest amounts of ...201122030412
occurrence of aeromonas spp. in a random sample of drinking water distribution systems in the usa.aeromonads are aquatic bacteria found in drinking water supplies worldwide. some species, such as aeromonas hydrophila, can cause disease in humans. for this survey, 293 united states public water systems were selected using random sampling, stratified by water source and system type. water samples were collected during one year from three sites (six samples per site) in each system. temperature, ph, turbidity, total and free chlorine were measured using standard methods. aeromonads were detecte ...201122048437
aeromonas aquariorum is widely distributed in clinical and environmental specimens and can be misidentified as aeromonas hydrophila.genotypic characterization of 215 aeromonas strains (143 clinical, 52 environmental, and 20 reference strains) showed that aeromonas aquariorum (60 strains, 30.4%) was the most frequently isolated species in clinical and water samples and could be misidentified as aeromonas hydrophila by phenotypic methods.201121697316
a novel amino-benzosuberone derivative is a picomolar inhibitor of mammalian aminopeptidase n/cd13.a new class of low molecular weight, highly potent and selective non peptidic inhibitors of aminopeptidase n (apn/cd13) is described. we report the synthesis and in vitro evaluation of racemic substituted analogues of 7-amino-benzocyclohepten-6-one 1a. we investigated various substitutions on the aromatic ring with phenyl and halogen groups. in vitro kinetic studies revealed that these compounds are among the most effective apn/cd13 inhibitors found so far. hydrophobic substituents placed at pos ...201121843945
extending the aerolysin family: from bacteria to vertebrates.a number of bacterial virulence factors have been observed to adopt structures similar to that of aerolysin, the principal toxin of aeromonas species. however, a comprehensive description of architecture and structure of the aerolysin-like superfamily has not been determined. in this study, we define a more compact aerolysin-like domain--or aerolysin fold--and show that this domain is far more widely spread than anticipated since it can be found throughout kingdoms. the aerolysin-fold could be f ...201121687664
[role of aeromonas in the monitoring of a hydroecosystem in the volga-caspian area].the paper gives data obtained over many years on the quantity of bacteria of the genus aeromonas in the hydroecosystems of the delta of the volga and caspian sea and in commercial fishes (sturgeon (acipenseridae), pike perch (stizostedion), carp (cyprinus carpio), silurus (silurus glanis), etc.). it provides a detailed analysis of their seasonal trends and spatial distribution, markers of their pathogenicity, and antibiotic resistance. the findings suggest that aeromonas is a causative agent of ...201121842729
independent predictors of mortality for necrotizing fasciitis: a retrospective analysis in a single institution.necrotizing fasciitis (nf), a life-threatening soft tissue infection, requires early diagnosis, prompt and repeated surgical intervention, and broad-spectrum antibiotic therapy. the aim of this study was to identify the independent predictors of mortality among patients with nf in taiwan.201121825948
[Phenotypic characteristics and virulence factors in Aeromonas strains isolated from patients with diarrheic disease in Cuba].Fifty four strains of Aeromonas spp were isolated from patients with acute diarrheic episodes by using Aerokey II and Aeroesquema methods. In vitro antimicrobial susceptibility and virulence factors were analyzed. The most frequently isolated specie was Aeromonas caviae. Over 75% of strains exhibited resistance to penicillins and ce-phalosporins; for the other antibiotic groups resistance was under 20%. Twenty six strains (48.1 %) were multiresist-ant. At least one virulence factor among those e ...201121720696
silent spread of imp-13-producing pseudomonas aeruginosa belonging to sequence type 621 in belgium. 201121693463
bacillus amyloliquefaciens g1: a potential antagonistic bacterium against eel-pathogenic aeromonas hydrophila.recent studies have revealed that the use of probiotics is an alternative to control marine aeromonas. however, few probiotics are available against aeromonas hydrophila infections in eels. in the present study, a potential antagonistic strain g1 against the eel-pathogenic a. hydrophila was isolated from sediment underlying brackish water. its extracellular products with antibacterial activities were shown to be stable under wide range of ph, temperature, and proteinase k. it was initially ident ...201121754944
selection for improved resistance to aeromonas hydrophila in indian major carp labeo rohita: survival and innate immune responses in first generation of resistant and susceptible lines.selection for disease resistance in fish may be performed directly on basis of survival data obtained in controlled challenge trials, or indirectly using information from immunological or molecular markers linked to differential survival. in the present study, several key innate immune parameters were measured in aeromoniasis resistant and susceptible lines of rohu labeo rohita to assess their suitability as immune markers for use in indirect selection for increased resistance. experimental infe ...201121712094
Aeromonas hydrophila-associated septicemia in captive crocodiles (Crocodylus johnstoni and Crocodylus porosus).Five 25-yr-old crocodiles (Crocodylus johnstoni and Crocodylus porosus) were diagnosed with Aeromonas hydrophila-associated septicemia accelerated by improper thermoregulation. At necropsy, pulmonary congestion and pleural effusion were the main lesions in the thorax. Necrotizing enteritis, intestinal hemorrhage, fibrinous serositis, hepatitis, and pancreatitis were observed in the abdominal cavities of all five crocodiles. Aeromonas hydrophila was identified in the pleural effusions and abdomin ...201122204074
protection against atypical aeromonas salmonicida infection in common carp, cyprinus carpio l., by oral administration of a mixed microbial culture of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae.a microbial culture was prepared by co-cultivation of lactobacillus paracasei, pichia membranifaciens and saccharomyces cereviciae for 48 hr at 30 c in rice bran extract medium, supplemented with dextrose. oral administration of the resulting non-viable heat-inactivated microbial culture to common carp, cyprinus carpio l., delivered in feed for four weeks, induced effective protection against experimental atypical aeromonas salmonicida infection which causes "ulcer disease". after challenge of t ...201121685718
Aeromonas salmonicida-secreted protein AopP is a potent inducer of apoptosis in a mammalian and a Drosophila model.Some pathogens are able to establish themselves within the host because they have evolved mechanisms to disrupt host innate immunity. For example, a number of pathogens secrete preformed effector proteins via type III secretion apparatuses that influence innate immune or apoptotic signalling pathways. One group of effector proteins that usurp innate immune signalling is the YopJ-like family of bacterial effector proteins, which includes AopP from Aeromonas salmonicida. Aeromonas species are know ...201122040305
a putative g protein-coupled receptor involved in innate immune defense of procambarus clarkii against bacterial infection.the immune functions of g protein-coupled receptor (gpcr) were widely investigated in mammals. however, limited researches on immune function of gpcrs were reported in invertebrates. in the present study, the immune functions of hp1r gene, a putative gpcr identified from red swamp crayfish procambarus clarkii were reported. expression of hp1r gene was significant up-regulated in response to heat-killed aeromonas hydrophila challenge. hp1r gene silencing mediated by rna interference significantly ...201121964155
formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in burkholderia sp.burkholderia sp. synthase has been shown to polymerize 3-hydroxybutyrate (3hb), 3-hydroxyvalerate, and 3-hydroxy-4-pentenoic acid monomers. this study was carried out to evaluate the ability of burkholderia sp. usm (jcm 15050) and its transformant harboring the polyhydroxyalkanoate (pha) synthase gene of aeromonas caviae to incorporate the newly reported 3-hydroxy-4-methylvalerate (3h4mv) monomer. various culture parameters such as concentrations of nutrient rich medium, fructose and 4-methylval ...201121279348
amino-benzosuberone: a novel warhead for selective inhibition of human aminopeptidase-n/cd13.this paper describes the design and synthesis of compounds belonging to a novel class of highly selective mammalian cd13 inhibitors. racemic homologues of 3-amino-2-tetralone 1 were synthesised and evaluated for their ability to selectively inhibit the membrane-bound, zinc-dependent aminopeptidase-n/cd13 (ec 3.4.11.2). some of these novel non-peptidic compounds are potent, competitive inhibitors of the mammalian enzyme, with k(i) values in the low micromolar range in spite of their minimal size ...201121292493
painful purple plaques on the thighs of an immunosuppressed patient. 201121418277
immunomodulatory effects of secondary metabolites from thermophilic anoxybacillus kamchatkensis xa-1 on carp, cyprinus carpio.a bacterial strain with putative immunomodulatory properties was isolated from xi'an hot springs in china. comparison of 16s rrna gene revealed a 97% similarity between the tested strain (designated xa-1) and anoxybacillus kamchatkensis. two compounds isolated from the secondary metabolites of xa-1 were identified by spectral data (infrared, nuclear magnetic resonance and mass spectrometry) as: (1) cyclo (gly-l-pro) and (2) cyclo (l-ala-4-hydroxyl-l-pro). two cyclic dipeptides showed stimulatory ...201121421057
new developments in traveler's diarrhea.traveler's diarrhea (td) is a crucial area for research, as it affects millions of tourists each year and creates a large economic burden. more than 60% of td cases are caused by a variety of bacterial enteropathogens: diarrhea-producing escherichia coli, shigella, campylobacter, salmonella, aeromonas, plesiomonas, and noncholera vibrios. noroviruses are also an important cause of morbidity among travelers. recent studies have identified host genetic risk factors associated with susceptibility t ...201121475415
dissemination of ndm-1 positive bacteria in the new delhi environment and its implications for human health: an environmental point prevalence study.not all patients infected with ndm-1-positive bacteria have a history of hospital admission in india, and extended-spectrum β-lactamases are known to be circulating in the indian community. we therefore measured the prevalence of the ndm-1 gene in drinking water and seepage samples in new delhi.201121478057
phylogenetic identification of aeromonas from pigs slaughtered for consumption in slaughterhouses at the north of portugal.in the present study, 710 isolates of aeromonas spp. have been collected from pig carcasses, diaphragm muscle, faeces, dehairing equipment and water in slaughterhouses at the north of portugal. the isolates were obtained from a total of 154 samples. all presumptive aeromonas isolates were subjected to eric-pcr analysis and those which presented a different pattern were taken and the species classified by gyrb gene sequencing. we have found the species a. hydrophila, a. salmonicida, a. bestiarum, ...201121402427
effect of enzyme producing microorganisms on the biomass of epigeic earthworms (eisenia fetida) in vermicompost.we analyzed the bacterial community structure of the intestines of earthworms and determined the effect of enzyme producing microorganisms on the biomass of earthworms in vermicompost. fifty-seven bacterial 16s rdna clones were identified in the intestines of earthworms by using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) analysis. entomoplasma somnilux and bacillus licheniformis were the dominant microorganisms; other strains included aeromonas, bacillus, clostr ...201121421302
molecular cloning, characterization and expression analysis of the complement component c6 gene in grass carp.the complement system, as a representative of innate immunity, plays a key role in the host defense against infections. c6 is the member of complement components creating the membrane attack complex (mac). in this study, we cloned and characterized the grass carp complement component c6 (gcc6) gene. our data showed that gcc6 gene contained a 2724bp open reading frame (orf), a 237bp 5'-untranslated region (utr) and a 219bp 3'-utr. the deduced amino acid sequence of gcc6 showed 77.6% and 58.9% ide ...201121353312
multilocus phylogenetic analysis of the genus aeromonas.a broad multilocus phylogenetic analysis (mlpa) of the representative diversity of a genus offers the opportunity to incorporate concatenated inter-species phylogenies into bacterial systematics. recent analyses based on single housekeeping genes have provided coherent phylogenies of aeromonas. however, to date, a multi-gene phylogenetic analysis has never been tackled. in the present study, the intra- and inter-species phylogenetic relationships of 115 strains representing all aeromonas species ...201121353754
antigen in chitosan coated liposomes enhances immune responses through parenteral immunization.to overcome the limitations of the use of conventional liposomes chitosan coated egg yolk lecithin based liposomes were used as antigen carrier through parenteral administration in two animal models such as fish and rabbit. extracellular proteins (ecps) antigen of aeromonas hydrophila encapsulated in liposomes became more stable and induced better immune response. it enhanced both adaptive and innate immune responses than other preparations both at 21, 42 and 63days post-immunization and suggest ...201121354354
microbiological quality of grey-mullet roe.the greek grey-mullet roe is produced from the fully developed gonads of the female mullet (mugil cephalus) couth in lagoons during their reproductive migration. the traditional processing method of the roe includes, air drying, salting, shape formation and covering with multiple layers of natural beeswax for preservation and distribution. fish roe brands have been a staple in local diet and is increasingly becoming popular in the international market. as a ready-to-eat food it's microbial quali ...201121497664
complex evolutionary history of the aeromonas veronii group revealed by host interaction and dna sequence data.aeromonas veronii biovar sobria, aeromonas veronii biovar veronii, and aeromonas allosaccharophila are a closely related group of organisms, the aeromonas veronii group, that inhabit a wide range of host animals as a symbiont or pathogen. in this study, the ability of various strains to colonize the medicinal leech as a model for beneficial symbiosis and to kill wax worm larvae as a model for virulence was determined. isolates cultured from the leech out-competed other strains in the leech model ...201121359176
molecular characterization of tetracycline- and quinolone-resistant aeromonas salmonicida isolated in korea.the antibiotic resistance of 16 aeromonas (a.) salmonicida strains isolated from diseased fish and environmental samples in korea from 2006 to 2009 were investigated in this study. tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. among the tetracycline-resistant strains, seven of the isolates harbored teta gene and one isolate harbored tete gene. additionally, quinolone-resistance determining ...201121368562
cloning and characterization of timp-2b gene in grass carp.tissue inhibitors of metalloproteinases (timps) are the primary inhibitors of matrix metalloproteinases (mmps) in tissues. in this study, a timp-2 gene was isolated from grass carp ctenopharyngodon idella (citimp-2b) and characterized. this cdna sequence encoded a signal peptide, n- and c-terminal domains. citimp-2b is highly homologous to the orthologous in zebrafish and other teleosts, suggesting timp-2b is highly conserved during teleost evolution. citimp-2b gene is expressed in a wide range ...201121377537
structural characterization of the o-specific polysaccharide from the lipopolysaccharide of the fish pathogen aeromonas bestiarum strain p1s.the o-specific polysaccharide obtained by mild-acid degradation of lipopolysaccharide of aeromonas bestiarum p1s was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy. the sequence of the sugar residues was determined using (1)h,(1)h noesy and (1)h,(13)c hmbc experiments. the o-specific polysaccharide was found to be a high-molecular-mass polysaccharide composed of tetrasaccharide repeating units of the structure [formula in text]. since small amounts of a term ...201121377659
common key acidogen populations in anaerobic reactors treating different wastewaters: molecular identification and quantitative monitoring.bacterial population dynamics during the start-up of three lab-scale anaerobic reactors treating different wastewaters, i.e., synthetic glucose wastewater, whey permeate, and liquefied sewage sludge, were assessed using a combination of denaturing gradient gel electrophoresis (dgge) and real-time pcr techniques. the dgge results showed that bacterial populations related to aeromonas spp. and clostridium sticklandii emerged as common and prominent acidogens in all reactors. two real-time pcr prim ...201121377710
molecular cloning and characterization of foxp3 in atlantic salmon (salmo salar).foxp3 is a t cell-specific transcription factor and plays a key role in the development of treg cells and in the immune regulatory process during inflammation. here we report cloning and characterization of the full-length cdna of atlantic salmon foxp3, which possesses a forkhead domain, a zinc finger domain and a leucine-zipper domain as its counterpart in mammals. foxp3 is highly expressed in thymus. furthermore, regulated expression was observed in head kidney cells in response to ß-glucan an ...201121276855
regulation of type vi secretion gene clusters by sigma54 and cognate enhancer binding proteins.type vi secretion systems (t6ss) are bacteriophage-derived macromolecular machines responsible for the release of at least two proteins in the milieu, which are thought to form an extracellular appendage. although several t6ss have been shown to be involved in the virulence of animal and plant pathogens, clusters encoding these machines are found in the genomes of most species of gram-negative bacteria, including soil, marine, and environmental isolates. t6ss have been associated with several ph ...201121378190
occurrence, abundance and elimination of class 1 integrons in one municipal sewage treatment plant.integrons are elements that encode a site-specific recombination system that recognizes and captures mobile gene cassettes and are closely related to multiple resistances of environmental microorganisms. this study was undertaken to determine the efficiency of an activated sludge process to remove integrons. the prevalence and characteristics of class 1 integrons were investigated for bacterial species isolated from the activated sludge of nanjing jiangxinzhou sewage treatment plant (stp, china) ...201121431316
dietary administration of bacillus thuringiensis on the cellular innate immune response of african catfish (clarias gariepinus) against aeromonas hydrophila.an experiment was conducted to evaluate the use of bacillus thuringiensis (bt) as a probiotic to enhance the cellular innate immune response of the african catfish (clarias gariepinus) challenged with a bacterial fish pathogen, aeromonas hydrophila.201121381499
aeromonas spp. induce apoptosis of epithelial cells through an oxidant-dependent activation of the mitochondrial pathway.we investigated interactions of aeromonas caviae, aeromonas veronii biotype sobria and aeromonas hydrophila strains, isolated from faecal specimens of humans with gastroenteritis, with ht29 intestinal epithelial cells. all strains were found to be cytotoxic to the cells. bacterial infection caused generation of reactive oxygen species (ros) and nitric oxide radical (no(-à)). the maximal levels of ros and no(-à) were 14 and 35 times, respectively, greater in cells infected with aeromonas spp. tha ...201121436367
diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan.a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ...201121487197
in silico analysis of 16s ribosomal rna gene sequencing based methods for identification of medically important aerobic gram-negative bacteria.the study is the first one that provides guidelines on the usefulness of full and 527-bp 16s rrna gene sequencing and microseq databases for identifying medically important aerobic gram-negative bacteria. overall, full and 527-bp 16s rrna gene sequencing can identify 26.1% and 32.6% of medically important aerobic gram-negative bacteria confidently to the species level, whereas the full-microseq and 500-microseq databases can identify 15.2% and 26.1% of medically important aerobic gram-negative b ...201121498652
effects of anthraquinone extract from rheum officinale bail on the physiological responses and hsp70 gene expression of megalobrama amblycephala under aeromonas hydrophila infection.we evaluated the effect of dietary supplementation with anthraquinone extract (from rheum officinale bail) on the resistance to aeromonas hydrophila infection in megalobrama amblycephala. the fish were randomly divided into two groups: a control group (fed a standard diet) and a treatment group (standard diet supplemented with 0.1% anthraquinone extract) and fed for 10 weeks. we then challenged the fish with a. hydrophila and recorded mortality and changes in serum cortisol, lysozyme, alkaline p ...201121362482
pyrosequencing demonstrated complex microbial communities in a membrane filtration system for a drinking water treatment plant.microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. sequences of 16s rrna gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) tha ...201121502735
[effect of heavy metal salts on the growth and pathogenicity factors of opportunistic bacteria].the paper presents experimental data on the effect of different concentrations of the salts of cadmium, copper, and zinc on the adaptive potentialities of the most common opportunistic microflora in the volga-caspian region. the findings suggest that natural microorganisms (aeromonas and citrobacter) show a high metal resistance that seems to be associated with steadily increased background levels of these metals in the waters of the volga-caspian basin.201121510046
phenotypic characteristics of human clinical and environmental aeromonas in western australia.: to determine the phenotypic characteristics of 199 aeromonas strains comprising 146 clinical and 53 environmental isolates.201121519288
production and properties of keratinolytic proteases from three novel gram-negative feather-degrading bacteria isolated from brazilian soils.the keratinolytic potential and protease properties of three novel gram-negative feather-degrading bacteria isolated from brazilian soils was described. aeromonas hydrophila k12, chryseobacterium indologenes a22 and serratia marcescens p3 were able to degrade feather meal, producing high amounts of soluble proteins and forming thiol groups. the proteases of strains k12, a22 and p3 had optimal ph of 8.0, 7.5 and 6.0, respectively; this last is an uncommon feature for bacterial keratinases. the op ...201121526391
involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila.cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ...201121539922
osteomyelitis associated to ctx-m-15-producing aeromonas hydrophila: first description in the literature. 201121546197
multidrug resistance in hydrocarbon-tolerant gram-positive and gram-negative bacteria.new gram-positive and gram-negative bacteria were isolated from poeni oily sludge, using enrichment procedures. the six gram-positive strains belong to bacillus, lysinibacillus and rhodococcus genera. the eight gram-negative strains belong to shewanella, aeromonas, pseudomonas and klebsiella genera. isolated bacterial strains were tolerant to saturated (i.e., n-hexane, n-heptane, n-decane, n-pentadecane, n-hexadecane, cyclohexane), monoaromatic (i.e., benzene, toluene, styrene, xylene isomers, e ...201121478643
isolation and characterization of virulent aeromonas veronii from ascitic fluid of oscar astronotus ocellatus showing signs of infectious dropsy.the cichlid oscar astronotus ocellatus has worldwide commercial value in the pet fish industry because of its early maturation, relatively high fecundity, ability to identify its caretaker and also to alter colouration amongst conspecifics. pathogenic strains of aeromonas veronii resistant to multiple antibiotics were isolated from a. ocellatus individuals showing signs of infectious abdominal dropsy. the moribund fish showed haemorrhage in all internal organs, and pure cultures could be obtaine ...201121553566
crystal structure of the heteromolecular chaperone, asce-ascg, from the type iii secretion system in aeromonas hydrophila.the putative needle complex subunit ascf forms a ternary complex with the chaperones asce and ascg in the type iii secretion system of aeromonas hydrophila so as to avoid premature assembly. previously, we demonstrated that the c-terminal region of ascg (residues 62-116) in the hetero-molecular chaperone, asce-ascg, is disordered and susceptible to limited protease digestion.201121559439
identification of unique dna sequences present in highly virulent 2009 alabama isolates of aeromonas hydrophila.in 2009, a disease outbreak caused by aeromonas hydrophila occurred in 48 catfish farms in west alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. virulence studies have revealed that the 2009 isolates of a. hydrophila are at least 200-fold more virulent than a 1998 alabama isolate al98-c1b. however, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of a. hydrophila. to understan ...201121561726
structural modification of sanguinarine and chelerythrine and their antibacterial activity.in this study, five derivatives of sanguinarine (1) and chelerythrine (2) were prepared, with 1 and 2 as starting materials, by reduction, oxidation and nucleophilic addition to the iminium bond c=n(+). the structures of all compounds were elucidated on account of their ms, (1)h-nmr and (13)c-nmr data. the antibacterial activities of all compounds were screened, using staphylococcus aureus, escherichia coli, aeromonas hydrophila and pasteurella multocida as test bacteria. the minimum bacteriosta ...201121491327
structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a qnr protein from a gram-negative bacterium.quinolones inhibit bacterial type ii dna topoisomerases (e.g. dna gyrase) and are among the most important antibiotics in current use. however, their efficacy is now being threatened by various plasmid-mediated resistance determinants. of these, the pentapeptide repeat-containing (prp) qnr proteins are believed to act as dna mimics and are particularly prevalent in gram-negative bacteria. predicted qnr-like proteins are also present in numerous environmental bacteria. here, we demonstrate that o ...201121227918
involvement of the gspab complex in assembly of the type ii secretion system secretin of aeromonas and vibrio species.the type ii secretion system (t2ss) functions as a transport mechanism to translocate proteins from the periplasm to the extracellular environment. the exea homologue in aeromonas hydrophila, gspa(ah), is an atpase that interacts with peptidoglycan and forms an inner membrane complex with the exeb homologue (gspb(ah)). the complex may be required to generate space in the peptidoglycan mesh that is necessary for the transport and assembly of the megadalton-sized exed homologue (gspd(ah)) secretin ...201121378198
directed culturing of microorganisms using metatranscriptomics.the vast majority of bacterial species remain uncultured, and this severely limits the investigation of their physiology, metabolic capabilities, and role in the environment. high-throughput sequencing of rna transcripts (rna-seq) allows the investigation of the diverse physiologies from uncultured microorganisms in their natural habitat. here, we report the use of rna-seq for characterizing the metatranscriptome of the simple gut microbiome from the medicinal leech hirudo verbana and for utiliz ...201121467263
k+ efflux, is required for histone-h3 dephosphorylation by listeria llo and other pore forming toxins.chromatin modification triggered by bacteria is a newly described mechanism by which pathogens impact host transcription. listeria monocytogenes dephosphorylates histone h3 through the action of listeriolysin o (llo), however, the underlying mechanism is unknown. here we show that an unrelated pore forming toxin, aeromonas aerolysin also provokes h3 dephosphorylation. as reported for aerolysin, we show that llo, and related toxins induce a pore-dependent k(+) efflux, and that this efflux is the ...201121482680
inductive expression of toll-like receptor 5 (tlr5) and associated downstream signaling molecules following ligand exposure and bacterial infection in the indian major carp, mrigal (cirrhinus mrigala).toll-like receptors (tlrs) are one of the key components of innate immunity. among various types of tlrs, tlr5 is involved in recognizing bacterial flagellin and after binding, it triggers myeloid differentiation primary response gene 88 (myd88)-dependent signaling pathway to induce pro-inflammatory cytokines. in this report, we analyzed the expression profile of tlr5 and its associated downstream signaling molecules like myd88 and tumor necrosis factor (tnf) receptor-associated factor (traf) 6 ...201222085689
The spoilage of air-packaged broiler meat during storage at normal and fluctuating storage temperatures.Bacterial diversity and the major flora present on air-packaged broiler meat during storage at normal (4°C) and fluctuating storage temperatures (0-4°C and 4-10°C) were investigated using culture-dependent and culture-independent approaches. Culture-dependent analysis revealed that the growth of microflora was retarded when broiler meat was stored at lower temperatures (0-4°C). Denaturing gradient gel electrophoresis profiles showed that Staphylococcus spp., Pseudomonas spp., Acinetobacter spp., ...201222184446
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...201222168454
Interactions of bacteria with different mechanisms for chitin degradation result in the formation of a mixed-species biofilm.In this study, interactions between bacteria possessing either released or cell-associated enzymes for polymer degradation were investigated. For this, a co-culture of Aeromonas hydrophila strain AH-1N as an enzyme-releasing bacterium and of Flavobacterium sp. strain 4D9 as a bacterium with cell-associated enzymes was set up with chitin embedded into agarose beads to account for natural conditions, under which polymers are usually embedded in organic aggregates. In single cultures, strain AH-1N ...201222092834
the two-component qsebc signalling system regulates in vitro and in vivo virulence of aeromonas hydrophila.we recently demonstrated that the n-acyl-homoserine lactone [autoinducer (ai)-1] and luxs (ai-2)-based quorum-sensing (qs) systems exerted positive and negative regulation, respectively, on the virulence of a diarrhoeal isolate ssu of aeromonas hydrophila. however, the role of a newly identified, two-component-based qsebc qs system in the regulation of bacterial virulence in general is not well understood, with only a limited number of studies showing its function in bacterial pathogenesis. in t ...201221998161
In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture.Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ...201222078751
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