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potential pathogens in the environment: isolation, enumeration, and identification of seven genera of intestinal bacteria associated with small green pet turtles.bacteriological analyses were performed on fecal swabs and the aquarium water of 27 individually purchased specimens of the small green pet turtle, pseudemys scripta elegans. representatives of aeromonas, citrobacter, enterobacter, klebsiella, proteus, salmonella, and serratia were isolated. enterobacter, klebsiella, and salmonella were encountered in 20% or more of the specimens, whereas aeromonas was isolated from 63%. klebsiella pneumoniae counts ranged from 10(3) to 10(4) per milliliter of a ...19734572984
medium for the isolation of aeromonas hydrophila.a new differential medium, rimler-shotts, was tested with 109 isolates representing 13 genera of bacteria obtained from aquatic environments and animals. this medium was effective in presumptive identification of the strains of aeromonas hydrophila examined, with 94% accuracy. strains of citrobacter which were hydrogen sulfide-variable could not be separated from a. hydrophila. this medium was designed to facilitate diagnosis of a. hydrophila infections in animals and humans.19734584594
genetic and phenetic classification of bacteria. 19734584677
immunological investigations of the structure and antigens of shigella sonnei and serologically related bacteria. 19734618438
aeromonas hydrophila septicemia in a dog. 19734692305
gas-forming aeromonas hydrophila infection in a diabetic. 19734721025
preparation of metabolically active staphylococcus aureus protoplasts by use of the aeromonas hydrophila lytic enzyme.stable, metabolically active protoplasts of staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by aeromonas hydrophila. respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 m sucrose, 0.37 m sodium succinate, or 0.37 m sodium sulfate, have been shown to be comparable with the same parameters in intact cells under the same conditions.19734728270
lipolytic bacteria in the ottawa river.lipolytic bacteria were isolated from two stations on brewery creek, an arm of the ottawa river, during the winter of 1971-72. total counts were approximately sevenfold higher at the more polluted downstream station, whereas lipolytic counts were about 100-fold higher. at this station, significantly more lipolytic bacteria grew on plates incubated at 20 c than at 4 c, suggesting that the population was comprised of both mesophiles and psychrophiles. however, at the upstream station, approximatel ...19734762394
essentiality of vitamin c in feeds for intensively fed caged channel catfish. 19734682446
aeromonas shigelloides bacteremia: a case report. 19734687623
flower vases in hospitals as reservoirs of pathogens. 19734127638
serological properties and amino acid composition of the fimbriae of aeromonas hydrophila. 19744135102
[cytochrome absorption spectra of bacteria as aid for solving taxonomic problems. 2. the redox and carbon monoxide difference spectra of bacterial species of the families enterobacteriaceae, and pasteurellaceae, and of the genera aeromomas, xanthomonas, vibrio, acinetobacter, alcaligenes, and achromobacter (author's transl)]. 19744152660
leucocidic substances for aeromonas hydrophila. 19744155852
[cytochrome absorption spectra of bacteria as aid for solving taxonomic problems. 3rd report: the cytochrome absorption spectra of the investigated bacterial strains and their relationships to the taxonomy (author's transl)]. 19744155853
[bacteriological investigations on reptiles and amphibians (author's transl)]. 19744155899
[furunculosis of the brook trouts]. 19744210314
isolation, enumeration, and host range of marine bdellovibrios. 19744211210
sensitivity of different pseudomonas species and aeromonas hydrophila to trimethoprim and sulfamethoxazole separately and in combination. 19744214032
microbiological studies on septicemic bullfrogs (rana catesbeiana). 19744214168
bacterial contamination and sparing heat treatment of mother's milk. 19744215294
effect of diethylstilbestrol on feedlot associated bacteria. 19744215508
microbial degradation of cholesterol. 19744219244
[ecology of facultatively pathogenic bacteria with low demands in nutritive substances in hospitals (author's transl)]. 19744219781
aeromonas hydrophila infections. 19744809188
[use of antibiotics in hemorrhagic septicemia of carp]. 19744450497
[change in the composition of the fatty acids in hemorrhagic septicemia of carp]. 19744450498
some aspects of the regulation of the production of extracellular proteolytic enzymes by a marine bacterium. 19744474857
the purification of aeromonas aminopeptidase by affinity chromatography. 19744831337
colonization patterns of aerobic gram-negative bacteria in the cloaca of rana pipiens. 19744362878
[two cases of aeromonas hydrophila septicemia associated with acute leukemia (author's transl)]. 19744533011
[microorganisms of aeromonas genus. pathogenicity for man, pathogenesis and epidemiology (review of non-soviet literature)]. 19744454822
single and continuous exposure of the adult american oyster, crassostrea virginica, to marine vibrios. 19744597645
comparative survival of indicator bacteria and enteric pathogens in well water.the comparative survival of various fecal indicator bacteria and enteric pathogens was studied in a stable well water supply by using membrane chambers. there was more variation in the 29 coliform cultures and they died more rapidly, as a group, than the 20 enterococcus cultures that were examined. the comparative survival of the organisms tested follows: aeromonas sp. > the shigellae (shigella flexneri, s. sonnei, and s. dysenteriae) > fecal streptococci > coliforms = some salmonellae (salmonel ...19744598219
[antibiotics. new aspects from the viewpoint of a microbiologist. assumptions for significant use of antibiotics]. 19744599415
the involvement of extracellular enzymes in the metabolism of bdellovibrio. 19744600233
applicability of cetrimide, nitrofurantoin, ttc, and sodium arsenite to the isolation of marine pseudomonads. 19744600732
occurrence of potential pathogens in water containing ornamental fishes.the bacterial population of the water supplied with ornamental fish purchased from retail outlets was examined qualitatively and quantitatively. as many as 10(9) viable aerobic organisms per 100 ml were present, with fecal coliform counts as high as 10(5) per 100 ml. citrobacter, escherichia, pseudomonas, and vibrio were isolated from 75% or more of the samples, whereas aeromonas, alcaligenes, enterobacter, flavobacterium, and streptococcus were isolated from 45 to 65% of the samples. pseudomona ...19744602309
the bacterial flora in the alimentary tract of freshwater salmonid fishes. 19744607659
incidence of hemolytic and r factors in lactose-fermenting enteric bacteria isolated from the digestive tract of fish. 19744612637
therapeutic trials with tobramycin. 19744613176
[a new medium for the rapid presumptive identification of enterobacteriae, aeromonas and vibrios (author's transl)]. 19744420397
fever in the lizard dipsosaurus dorsalis. 19744431469
present status of heiberg groups for classifying cholera-like organisms. 19744435858
microbial contamination of renal dialysis systems and associated health risks. 19744450335
effect of four dispersants on biodegradation and growth of bacteria on crude oil.four chemical dispersants, corexit 8666, gamlen sea clean, g. h. woods degreaser-formula 11470, and sugee 2 were examined singly and in individual combinations with arabian crude oil (1:1 ratio) at 10 and 25 c for their effects on the growth of bacteria indigenous to local marine waters, the bacterial population composition, and biodegradation of crude oil; in addition, their emulsifying capacities, at approximately 24 c, were determined. none of the dispersants used alone were toxic even at rel ...19744418491
[bacteriological diagnosis of aeromonas salmonicida infections in fishes]. 19744441360
kinetics of inhibition of aeromonas aminopeptidase by leucine methyl ketone derivatives. 19744441102
characterization of anti-aeromonas salmonicida antibodies from coho salmon.coho salmon (oncorhynchus kisutch) anti-aeromonas salmonicida agglutinins were excluded from sephadex g-200 and their activity was lost after partial reduction and alkylation followed by exposure to 2 m urea. immunoelectrophoretic analysis of coho antiserum revealed anodic and cathodic populations of immunoglobulin (ig), and the anti-a. salmonicida antibodies present 12 weeks after parenteral immunization were primarily anodic ig. antibodies were specifically purified by dissociation of a. salmo ...19744816458
[psychrophilic organisms in poultry meat]. 19744418360
effect of sodium chloride on growth of heterotrophic marine bacteria. 19744837198
aeromonas hydrophila wound infection. 19744855618
the characterization and significance of plesiomonas shigelloides and aeromonas hydrophila isolated from an epidemic of diarrhoea. 19744435916
[hemorrhagic septicemia of sliver carp]. 19744281147
partial characterization of aerolysin, a lytic exotoxin from aeromonas hydrophila.conditions are defined for the production, in high titers, of an extracellular hemolytic toxin of aeromonas hydrophila, here termed "aerolysin." substantial purification of the toxin was accomplished by means of salt fractionation, dialysis, and gel filtration, with a yield of 24% of the starting activity. analysis of the product by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed a single, heavy protein band and a number of faint protein bands. the estimated ...19744208526
[the methylene-blue-reduction-test (mr-test) and the micro-ttc-test for the determination of substances as sole source of carbon in the taxonomy of pseudomonas species (author's transl)].two methods are described for the determination of substances as sole source of carbon for taxonomic tools: the methylene-blue-reduction-test and the micro-ttc-test. both methods are giving results superior to the technique introduced by stanier and co-workers and used hitherto in the taxonomic classification of pseudomonas species. the evaluation of the comparative test results is based on the criterion that a substance is useful for taxonomic purpose provided that at least 90 per cent of the s ...197551553
[medium for determining the decarboxylase activity of vibnions]. 197552753
interactions between aerolysin, erythrocytes, and erythrocyte membranes.aerolysin, a hemolytic and lethal exotoxin of aeromonas hydrophila, was analyzed for amino acids. assuming 8 histidine residues/mol, the purified toxic protein has, by summation, a molecular weight of 49,000, a value in agreement with earlier estimates by other methods. erythrocytes from different animal species differ greatly in sensitivity to aerolysin's lytic action. there is some correlation between sensitivity and phosphatidyl choline content. erythrocyte membranes of different species bind ...1975166917
food poisoning--four unusual episodes.four unusual outbreaks of food poisoning occurring in the dunedin health district during the period 1971-1973 are described. these involved a contaminated cordial, a death associated with a clostridium perfringens outbreak, salmonellosis and infectious hepatitis in persons eating uncooked shellfish and symptoms associated with the ingestion of a normally edible fish--the trumpeter.1975170567
history and present status of fish diseases. 1975172662
[aeromonas punctata subsp. caviae as the causative agent of acute gastroenteritis (author's transl)].since in the past, aeromonas hydrophila had been isolated from all cases of human infection described. a. punctata and the anaerogenic sub-species were considered as apathogenic. from the case described, a close association between acute diarrhea with vomiting and the identification of a. punctata subsp. caviae becomes evident so that a conditional pathogenicity of this sub-species must be assumed. the question is discussed whether a preceding disturbance of the intestinal habitat in the presenc ...1975175615
the mode of regulation of bacterial citrate synthase as a taxonomic tool. 1975168310
vibrio cholerae flagellar antigens: a serodiagnostic test, functional implications of h-reactivity and taxonomic importance of cross-reactions within the vibrio genus.serodiagnostic tests for all serotypes of vibrio cholerae using h-antisera were investigated. activity motile cell lines of 155 stock and international reference cultures of human, animal, fish, and halophilic vibrios, aeromonas, comomonas, pseudomonas, salmonella, and escherichia were investigated. without exception, all cholera vibrios (including the nag serotypes) reacted with h sera. positive reactions were obtained specifically (a) within 2 hrs at 52 degrees c in the tube test using thick f ...197555952
chondroitinase-producing bacteria in natural habitats.a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...1975803822
an improvement in the technique for the presumptive identification of aeromonas salmonicida. 1975803942
rapid detection and identification of enteric bacteria from blood cultures.the bactec radiometric system and inolex enteric i card were used in conjunction for the rapid detection and presumptive identification of enteric gram-negative rods in blood cultures. excellent correlation was obtained between the inolex card and routine identification techniques when organisms from both artificially inoculated and clinical blood cultures were studied. in many instances, the culture report was available less than 24 hr after receipt of the specimen.1975804025
in-vitro effect of edta-tris-lysozyme solutions on selected pathogenic bacteria.the in-vitro effect of edta-tris-lysozyme solution on 16 pathogenic bacteria of medical or veterinary importance was determined. marked decreases in bacterial count occurred with pseudomonas aeruginosa, escherichia coli, moraxella osloensis and campylobacter fetus, and smaller decreses with salmonella typhimurium, shigella boydii, aeromonas hydrophila, proteus mirabilis, listeria monocytogenes and erysipelothrix insidiosa. the test solution had no effect on klebsiella ozaenae, brucella canis, co ...1975805241
brown- and red-pigmented pseudomonas aeruginosa: differentiation between melanin and pyorubrin.the pigment produced by three clinically isolated strains of pseudomonas aeruginosa has been shown to by pyomelanin. the pigment is also produced by three strains of the same species labelled as "var. erythrogenes" by the national collection of type cultures, and by aeromonas salmonicida. melanin and pyorubrin may be distinguished by the differential effects of tyrosine and glutamate on their production in minimal salts medium; furunculosis agar is a suitable medium for differentation of these p ...1975805242
[therapeutic effect of lividomycin on infections of the respiratory tract (author's transl)].therapeutic effect of lividomycin was observed in patients with infections of the respiratory tract (infections in association with bronchiectasis, mixed infections of cavities after negative conversion of tubercle bacilli, pneumonia, and so forth). significant improvement of clinical symptoms was observed in 15 cases and no improvement in 4 cases. lividomycin was administered at a dose of 1 g/day for 3 to 15 days. staphylococcus aureus (9 cases), klebsiella pneumoniae (1 case), alpha-hemolytic ...1975807755
bacteriophage typing of gram-negative rod-shaped bacteria. 1975811424
enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity.in or on agar media, low-density seedings of enterobacteria fail to grow in the presence of certain concentrations of ethylene diamine-di-orthohydroxyphenyl acetic acid (edda); on the other hand, high-density seedings not only grow but secrete iron chelators which release the iron bound by the edda in the medium and stimulate the growth of low-density seedings. plates of media containing edda with low-density seedings of indicator organisms were used to survey iron-chelator production in seven e ...1975812996
possible mis-identification of the fish pathogen aeromonas salmonicida. 1975813814
[investigations on the standardization of polytropic substances used in microbiological test procedures. i. suitability of vitamin-free casein hydrolysates as standardizable source of n and c for bacterial growth (author's transl)].comparative testing of vitamin-free casein hydrolysates produced by merck, difco and sheffield showed that the products are evidently fairly well standardised by the manufacturing specifications and satisfy requirements as polytropic source of n and c for microbiological test procedures in which high degree of reproducibility is demanded. tests were conducted with strains of e. coli, a. punctata, p. aeruginosa and myxobacter sp. and showed that differences within the trial groups in the colony c ...1975808926
methyl violet: a selective agent for differentiation of klebsiella pneumoniae from enterobacter aerogenes and other gram-negative organisms.three selective media for differentiation of klebsiella pneumoniae from enterobacter aerogenes on the basis of colonial morphology were evaluated. using methyl violet 2b as a selective agent, strains of k. pneumoniae isolated from urine, fresh water, and fresh produce were tested against other members of enterobacteriaceae in addition to strains of aeromonas hydrophila and pseudomonas aeruginosa. comparison of colonial morphology showed k. pneumoniae produced larger, smoother colonies than other ...1975809008
[testing of various aeromonas strains isolated from corps using the resistance test]. 19751091447
[swarming phenomenon of an aeromonas spec (author's transl)].a genuine swarming phenomenon, such as has previously been known to occur in proteus, bacillus and clostridium species only, was observed in an aeromonas species. fig. 1 shows the terraced swarming zones of the aeromonas species on nutrient agar. the swarming rate, expressed as the growth of the swarming zone per time unit, was measured to be 70-120 mum/min on blood agar at 30 degrees c. the swarming could be inhibited by incubation at 37 degrees c (table 2), by low saline concentrations (table ...19751101579
[utilization of lin (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria].a multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. the medium has the following formula: bacto-peptone (difco) 5 g; yeast extract (difco) 3 g; casein tryptic digest peptone (merek) 15 g; glucose 1 g; l-lysine-monohydrochloride (merck, no. 5700) 5 g; nacl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final ph 6.6. the medium is dispensed in amounts of 5 ml into tubes of 14 x 85 mm and autoclaved at 120 degrees c for 10 ...19751101584
reduced nitrofuran sensitivity conferred by r factors. 19751104937
tryptophan biosynthetic pathway in the enterobacteriaceae: some physical properties of the enzymes.several physical properties of the first four enzymatic activities of the tryptophan pathway were examined using gel filtration and ion exchange chromatography. five different patterns were noted. differences in the anthranilate synthetase (as) and phosphoribosylanthranilate transferase (prt) defined these patterns. in all the organisms studied phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase co-eluted from both diethylaminoethyl-cellulose and g-200 and thus probably ...19751116988
the occurrence of twitching motility among gram-negative bacteria.almost 1000 strains representing well above 50 different species or groups of gram-negative bacteria were examined for twitching motility. this kind of motility was mainly found in strictly aerobic cocci and rods (viz. acinetobacter calcoaceticus, moraxella spp., neisseria gonorrhoeae, n. meningitidis, pseudomonas spp., phenon 3 of thornley that is closely related to acinetobacter, and marine, yellow-pigmented rods), but also in the facultative eikenella corrodens and in anaerobic strains presum ...19751155115
effects of chemotherapeutics on bacterial ecology in the water of ponds and the intestinal tracts of cultured fish, ayu (plecoglossus altivelis).drug-resistant gram-negative bacilli conferred with r factors were isolated with high frequencies from the intestinal tracts of ayu (plecoglossus altivelis) cultured in ponds, in which chemotherapeutics had often been used, and with relatively low frequencies from ayu which received no administration of chemotherapeutics. drug-resistant bacteria were also isolated at low frequencies from the intestinal tracts of wild ayu in rivers, as well as from the water of ayu-culturing ponds and some of the ...19751160204
characterization of three aeromonas and nine pseudomonas species by extracellular enzymes and haemolysins.strains from type culture collections and clinical isolates belonging to the aeromonas and pseudomonas genera were identified with conventional tests. production of extra-cellular enzymes and haemolysins were detected by simple plate agar methods. the following enzymes were found to be of special value for a rapid and simple classification of certain species in both genera: potease (casein and gelatin agar), lecithinase (lecithin agar), and deoxyribonuclease (dna agar). elastase, staphylolytic e ...19751170482
letter: human infection with aeromonas species: varied clinical manifestations. 19751180435
the rapid serological diagnosis of fish furunculosis caused by "smooth" and "rough" strains of aeromonas salmonicida. 19751113076
fever and survival.the significance of fever in response to a bacterial infection has been investigated using the lizard dipsosaurus dorsalis as an animal model. these lizards develop a fever of about 2 degrees c after injection with the bacterium aeromonas hydrophila. to determine whether this elevation in body temperature increases the resistance of the host to this infection, as measured by survival, lizards were infected with the live bacteria and placed in a neutral (38 degrees c), low (34 degrees or 36 degre ...19751114347
a preliminary investigation of the effects of salinity on the bacterial flora of the tay estuary.two features of the effects of salinity on the bacterial flora of the tay estuary are considered. first the effect of salinity on the size and species composition of the resident microflora at various sites within the estuary. the second part is concerned with data obtained from laboratory experiments designed to study the effects of subjecting marine and freshwater bacteria, isolated from the tay, to salt stress.19751208501
[nutrition and taxonomy of "enterobacteriaceae" and related bacteria. iii. nutritional characters and differentiation of the taxonomic groups (author's transl)].a batch of 186 strains belonging to the families enterobacteriaceae or vibrionaceae has been studied by determination, for each strain, of the "versatility" towards 146 organic substrates tested as sole source of carbon and energy. this study allowed to work out a classification of these strains into the 32 classes which have been previously described. in the present paper the nutritional characters of these classes are reported. on the basis of these characters, the 32 classes may be regrouped ...19751217784
[incompatibility grouping of r factor detected from aeromonas hydrophila and a. salmonicida]. 19751240266
[demonstration of specific antibodies in sera of patients with infections caused by aeromonas hydrophila (author's transl)].according to the formation of specific antibodies against aeromonas hydrophilia in patients with infections caused by this microorganism the sera of two patients of different clinical picture were investigated. among the antibodies there could be demonstrated the antihaemolysins against the haemolysin of the monolgous strain, agglutinins against the o-antigen and praecipitins against soluble antigens. the special problems of the technics are described. the results are discussed and interpreted.19751240712
studies on the so-called nag vibrios. 19751213771
detection of aeromonas salmonicida antigen in diseased fish tissue. 19751171156
s-2,3-dicarboxy-aziridine, a new metabolite from a streptomyces. 19751184472
competitive ability of amylolytic bacteria in activated sludge.shifts were induced into the microbial community of activated sludge by the pulse addition of soluble starch. the subsequent changes of amylolytic and proteolytic microbial populations were recorded. four amylolytic strains were isolated and characterized with regard to carrying capacity, specific surface and growth kinetics. the competitive ability of these strains was studied by means of two-member competition experiments. these experiments were analysed according to the lotka-volterra model a ...19751190951
antibiotic sensitivity of two aeromonas and nine pseudomonas species.an agar dilution method was used to determine the in vitro sensitivity of different pseudomonas and aeromonas species to sulphonamide, tetracycline, colistin, gentamicin, tobramycin, amplicillin, carbenicillin, and cephalothin. p. aeruginosa was generally sensitive to carbenicillin, colistin, tobramycin, and gentamicin. p. putida and p. fluorescens were generally resistant to beta-lactam antibodies but sensitive to gentamicin and tobramycin. p. cepacia and p. maltophilia were mostly resistant to ...19751040755
recurrent aeromonas sepsis in a patient with leukemia.a case of recurrent sepsis due to aeromonas hydrophila in a patient with acute myelogenous leukemia is reported. the patient's first infection leading to bacteremia followed contamination of a mosquito bite by stagnant water. after recovery from the first bacteremia, the patient again became septic with a second strain of aeromonas hydrophila, which again responded to antimicrobial therapy. it is hypothesized that contamination of the local water supply may have led to the establishment of a gas ...19751060378
bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy.bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled.19751090258
enteropathogenicity of aeromonas hydrophila and plesiomonas shigelloides.aeromonas hydrophila was enteropathogenic in ligated ileal loops of rabbits, causing a fluid accumulation of 1-0 - 2-0 ml per cm of gut length. gut reaction could be produced with an inoculum as low as 10-4 viable bacteria. there was no difference in the nature of the positive reactions given by strains isolated from diarrhoeal and non-diarrhoeal children and adults and from water. plesiomonas shigelloides, on the other hand, did nt cause a significant gut reaction. a. hydrophila multiplied in t ...19751092866
microbial conversion of anthracycline antibiotics. 19751102512
rapid identification of gram-negative rods using a three-tube method combined with a dichotomic key.a three-tube method combined with a dichotomic key is presented which will identify virtually all enterobacteriaceae as well as a number of other frequently encountered gram-negative rods at a genus or species level within 20 hours following the primary isolation. the method is shown to be reliable and simple, saving processing time and material.19751106114
a cluster analysis of some bacteria in the water column of green lake, washington.mass inoculation and computer clustering techniques were used in an abbreviated procedure to group similar bacteria isolated from four depths in the water column of green lake, washington. four groups of bacteria were differentiable and were catagorized as orange-yellow flavobacterium-cytophaga, yellow flavobacterium-cytophaga, vibrio-aeromonas-, and pseudomonas-like. some of the groups were most prevalent in certain sample depths in the water column.19751116047
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