Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from aeromonas hydrophila.the structural gene for excreted amylase from aeromonas hydrophila jmp636 has been cloned within a 2.1-kilobase smai fragment of dna. the amylase gene is transcribed from its own promoter in escherichia coli, producing a gene product of mr 49,000. the amylase gene product is secreted to the periplasm of e. coli; however, it is not excreted. nucleotide sequencing revealed an open reading frame of 1,392 base pairs corresponding to a protein of 464 amino acid residues. a potential signal peptide of ...19882449422
comparison of four hemolysin-producing organisms (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) for release of inflammatory mediators from various cells.we investigated the role of various hemolysin-producing strains (escherichia coli, serratia marcescens, aeromonas hydrophila, and listeria monocytogenes) in induction of inflammatory mediators, e.g., histamine release from rat mast cells as well as the chemiluminescence response and the release of lipoxygenase transformation products from human polymorphonuclear neutrophils. our data show that the hemolysin-positive bacteria as well as the hemolysin-positive culture supernatants were active in i ...19882451679
gastro-enteritis in australian children: studies on the aetiology of acute diarrhoea.a prospective, year-long study of the microbiology of childhood gastro-enteritis in perth showed that (i) aeromonas-associated diarrhoea was the commonest recognized bacterial cause for acute diarrhoea, especially in summer, (ii) campylobacter infections were the next most common bacterial cause, (iii) rotavirus and parasites were not significant causes of sporadic acute childhood diarrhoea, and (iv) enteric bacterial pathogens and intestinal parasites were often isolated from aboriginal childre ...19882456732
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...19882459066
nucleotide sequence and transcriptional analysis of the aercaera region of aeromonas sobria encoding aerolysin and its regulatory region.the nucleotide sequence of a 2510 base pair chromosomal fragment containing the aerolysin gene aera, and its regulatory region aerc, from a clinical isolate of aeromonas sobria was determined. the aerolysin gene coded for a 54.5 kd polypeptide and had a g + c content of 59%, indicating that it is endogenous to the genus aeromonas. in contrast, the aerc region was characterized by its high a + t content (61%) and the presence of a core motif, aataaaa, repeated eight times within 300 base pairs. a ...19882459581
pathogenicity of aeromonas sp. for balb/c mice. 19883130510
purification and characterization of salmolysin, an extracellular hemolytic toxin from aeromonas salmonicida.an extracellular hemolytic toxin of aeromonas salmonicida, termed salmolysin, was purified 945-fold by ammonium sulfate precipitation, anion-exchange chromatography on deae-cellulose, and gel filtration chromatography on sephadex g-100 and sepharose 2b. salmolysin appeared homogeneous upon cellulose acetate membrane electrophoresis and immunodiffusion analysis. the molecular weight of the toxin was estimated to be approximately 200,000 by the sedimentation equilibrium method. the uv absorption s ...19883136147
wound infection with aerogenic aeromonas strains: a review of twenty-seven cases.aeromonas spp. have been implicated in a wide spectrum of human disease including wound infection. a review is made of local experience of 27 wound infections from which aerogenic strains of aeromonas were recovered; of these, aeromonas was isolated in pure culture from five infections. twenty-five infections were related to trauma and were community acquired, while two were probably nosocomial. of the 25 community acquired infections, 16 were associated with contamination by soil and one with w ...19883137035
[development of aeromonas hydrophila septicemia in a young healthy woman]. 19883138333
acute diarrhea in children in shanghai. 19883138335
aeromonas schubertii, a new mannitol-negative species found in human clinical specimens.in 1983 the vernacular name enteric group 501 was coined for a group of strains that had been referred to our laboratory as "possible vibrio damsela that does not require nacl for growth." by dna-dna hybridization (hydroxyapatite method, 32p, 60 and 75 degrees c), six strains of enteric group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees c and 71 to 93% at 75 degrees c; 0 to 1% divergence). type strains of all aeromonas species and reference strains of six other ...19883139706
[disease of the fins of rhamdia sapo. isolation of the etiological agents and experimental infection].during intensive culture of south-american catfish, rhamdia sapo fingerlings an epizootic similar to fin rot was developed. aeromonas hydrophila and pseudomonas aeruginosa were isolated and they were treated and controlled with daily short dip, 1 ppm furanace. the disease was experimentally reproduced and we concluded that it is result of interaction of unfavorable environment conditions such as: low dissolved oxygen, increase of solids in suspension; host condition and the presence of aeromonas ...19883140294
aeromonas species in a municipal water supply of a central european city: biotyping of strains and detection of toxins.in the course of a one-year study, 78 out of 239 samples from a central european municipal water supply were found to contain aeromonas species. biotyping and hemolysin titers according to the criteria of burke et al. (1) showed that almost 50% of the tested strains can be presumed to be enterotoxin-producing. the remaining microbiological parameters met international standards (20).19883140537
evaluation of 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) in broth and agar media for identification of pseudomonas aeruginosa.brain heart infusion broth and mueller-hinton agar containing the compound 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) were evaluated as selective media for identifying pseudomonas aeruginosa. of the 192 pseudomonas spp. and 68 additional oxidase-positive or glucose-nonfermenting gram-negative organisms tested, only p. aeruginosa (120 of 121 isolates) and certain strains of pseudomonas cepacia, aeromonas hydrophila, and achromobacter xylosoxidans wer ...19883141473
experimental evidence for enteropathogenicity in aeromonas veronii.eleven ornithine-positive strains of aeromonas (9 a. veronii and 2 provisionally classified as aeromonas species ornithine positive) were tested for ability to cause fluid accumulation in the rabbit ileal loop. all eight beta-hemolytic strains caused fluid accumulation. gel diffusion analysis revealed that the a. veronii beta-hemolysin was serologically related to the a. hydrophila beta-hemolysin, a known enterotoxic molecule. the biological activity of the a. veronii hemolysin was neutralized b ...19883143475
comparative in vitro activity of the new difluoro-quinolone temafloxacin (a-62254) against bacterial isolates from cancer patients.the in vitro activity of temafloxacin, a new difluoro quinolone agent, against 725 bacterial isolates representing 32 species was evaluated in comparison with that of ciprofloxacin. temafloxacin inhibited the majority of enterobacteriaceae isolates at a concentration of less than or equal to 0.5 microgram/ml. it was also extremely active against acinetobacter spp. and aeromonas hydrophila. its activity was 2-8 fold less than that of ciprofloxacin against most gram-negative isolates. methicillin- ...19883143585
bacterial hemolysins as virulence factors. 19883144241
[evaluation of 2 culture media for the isolation and enumeration of motile aeromonas in different kinds of water].in recent years an increasing incidence of aeromonas-related cutaneous infections and gastroenteritis has raised a serious public health problem. it appeared therefore timely to define a specific method allowing the rapid isolation and enumeration of the bacteria in their various aquatic habitats. in this line of research we have compared the growth of aeromonas originating from different aquatic sources and raised on two media, i.e. rs-agar and pxa-agar. whatever the aquatic system we observed ...19883144424
properties of aeromonads and their occurrence and hygienic significance in drinking water.in the netherlands, aeromonads in drinking water have attracted much attention in recent years. this development was caused by a sudden increase of the aeromonas density in the drinking water of the municipal dune waterworks of the hague and reports about the possible health significance of these organisms in drinking water. literature data indicate that representatives of the motile aeromonas species a. hydrophila, a. sobria and a. caviae generally have been observed in larger percentages of di ...19883146172
aeromonas hydrophila infection after cockles ingestion. 19883148523
in vitro activity of fleroxacin (ro23-6240), a new fluorinated 4-quinolone against isolates from cancer patients.the in vitro activity of fleroxacin (ro23-6240; am 833), a new fluorinated 4-quinolone, was compared to that of ciprofloxacin, enoxacin and a-56620, against 747 isolates from cancer patients. fleroxacin inhibited more than 90% of enterobacteriacea isolates at a concentration of less than or equal to 0.25 micrograms/ml. it was also extremely active against aeromonas hydrophila and haemophilus influenzae isolates with mic90 values of 0.12 and 0.06 micrograms/ml, respectively. the mic90 for acineto ...19883149568
immunoglobulin binding by the regular surface array of aeromonas salmonicida.the cell surface of aeromonas salmonicida is covered by a regular surface array composed of a single species of protein, the a-protein (phipps, b. m., trust, t. j., ishiguro, e. e., and kay, w. w. (1983) biochemistry 22, 2934-2939). the array, known as the a-layer, is the key virulence factor for this organism. cells containing the a-layer specifically bound rabbit igg and human igm with high affinity (kd = 1.0 x 10(-6) m and 3.3 x 10(-6) m, respectively), but neither isogenic a-protein-deficien ...19882967820
virulence properties and enterotoxin production of aeromonas strains isolated from fish.the biological activities in vivo and in vitro of 59 motile aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. a total of 64.3% of the isolates tested were pathogenic for fish, and 62% of aeromonas hydrophila and a. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. although the majority of the strains were proteolytic and amylolytic and produced dnase, other activities, such as elastase and staph ...19882972627
in vitro activity of lomefloxacin (sc-47111; ny-198), a difluoroquinolone 3-carboxylic acid, compared with those of other quinolones.lomefloxacin (sc-47111; ny-198) is a new difluoroquinolone agent. it inhibited 90% of escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, morganella morganii, proteus vulgaris, serratia marcescens, salmonella spp., shigella spp., aeromonas spp., yersinia spp., haemophilus influenzae, and neisseria gonorrhoeae at less than or equal to 2 micrograms/ml. lomefloxacin inhibited 90% of pseudomonas aeruginosa at 4 micrograms/ml. lomefloxacin was equal in activity ...19883164987
aeromonas hydrophilia infection in burn patients.aeromonas hydrophilia rarely infects burn wounds. three cases of early a. hydrophilia burn wound infection, with one death, are reported. a history of extinguishing the fire with dirty water or by rolling in dirt should alert the physician to consider a. hydrophilia as a possible infection organism.19883167587
suppurative thrombophlebitis due to aeromonas.a patient developed lethal suppurative thrombophlebitis and adjacent soft-tissue infection caused by aeromonas. potential risk factors included corticosteroid therapy and the use of warm tap water compresses at the site of intravenous catheter-related phlebitis. this case demonstrates the rapidly invasive characteristics of aeromonas and the need for early surgical intervention in suppurative thrombophlebitis.19883168427
dna:dna reassociation analysis of aeromonas salmonicida.dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...19883171541
[the occurrence of aeromonas hydrophila in birds]. 19883176762
isolation and characterization of motile aeromonas from human, food and environmental specimens.from july 1985 to march 1987, the occurrence of motile aeromonas sp. in stool, food and environmental specimens was investigated to assess their pathogenic significance and to determine sources and routes of infection. a total of 9366 stool specimens were examined; aeromonas was isolated from 11.1% of diarrhoeal stools and 2.2% of normal stools (p less than 0.001). aeromonas counts in food specimens, which included minced beef, pork and chicken, seafood and various vegetables and their products, ...19883181307
the in-vitro activity of pd127,391, a new quinolone.mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...19883182416
the comparative in-vitro activity of ofloxacin.the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...19883182468
comparison of blood agar, ampicillin blood agar, macconkey-ampicillin-tween agar, and modified cefsulodin-irgasan-novobiocin agar for isolation of aeromonas spp. from stool specimens.the performance of four media for the isolation of aeromonas strains from stool specimens, the importance of ampicillin-susceptible aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for aeromonas strains were evaluated in two phases. in the first phase, 36 of 1,672 stool specimens yielded aeromonas isolates. ninety-seven percent of the isolates were detected on blood agar containing 20 micrograms of ampicillin per ml (ab ...19883183020
[aeromonas hydrophila dacryocystitis. case report].the present paper reports on a case of severe dacryocystitis with a dacryolith, caused by aeromonas hydrophila. on the basis of this case the authors show that a therapy effective in vitro does not lead to elimination of bacteria in all cases, so that further therapeutic considerations are necessary.19883184754
aeromonas-associated diarrhea in children.in a 27-month prospective study, aeromonas spp. were isolated from 7.3% of children with diarrhea and from 2.2% of controls. in 32 patients with diarrhea, ranging in age from 1 to 27 months old, aeromonas spp. were the only potential bacterial enteropathogens isolated. principal symptoms of aeromonas-associated diarrhea were vomiting, fever and bloody stools. diarrhea was often self-limiting and lasted for 10 days or less in 90% of patients. no secondary spread of diarrhea among close contacts w ...19883186340
biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish.in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ...19883188376
the humoral immune response of rainbow trout (salmo gairdneri) immunised by various regimes and preparations of aeromonas salmonicida antigens.antibody production in rainbow trout to extracellular antigens was investigated. the following antigen preparations and immunisation regimes were used: native extracellular products (ecp) in freund's complete adjuvant (fca), intraperitoneally (i.p.) with and without booster; formalinized ecp in fca, i.p. with and without booster; washed, formalinized a. salmonicida cells in fca, i.p., with booster; native ecp in saline, i.m., four weekly injections at two different doses, 45 micrograms and 6 mic ...19883188377
in vitro activity of u-76,252 (cs-807), a new oral cephalosporin.u-76,252 is the prodrug of u-76,253. mics of u-76,253 were 0.015 to 0.06 microgram/ml for greater than or equal to 90% of the strains of streptococcus spp., haemophilus influenzae, and proteus mirabilis; 0.25 to 1 microgram/ml for branhamella catarrhalis, escherichia coli, klebsiella spp., and citrobacter diversus; 1 to 8 micrograms/ml for staphylococcus spp.; and 2 to greater than 16 micrograms/ml for other members of the family enterobacteriaceae and aeromonas hydrophila; for 72% of the latter ...19883190194
selection for virulence in the fish pathogen aeromonas salmonicida, using coomassie brilliant blue agar.coomassie brilliant blue agar was used to quantify the frequency of the a-layer phenotype in different isolates of aeromonas salmonicida. hydrophilic, non-clumping isolates of a. salmonicida consisted predominantly of the a-layer minus phenotype. these bacteria were avirulent by intraperitoneal injection into susceptible brook trout (salvelinus fontinalis) and could not be reisolated from infected fish. by contrast, hydrophobic, clumping isolates were predominantly of the a-layer positive phenot ...19883193563
inhibitory activity of antibiotic-producing marine bacteria against fish pathogens.the activity of antibiotic-producing marine bacteria was assayed against bacterial fish pathogens belonging to the genera vibrio, aeromonas, pasteurella, edwardsiella, yersinia and pseudomonas with the aim of evaluating the possible use of these marine strains for controlling epizootics in aquaculture. inhibition tests on solid medium showed that, in general, the majority of fish bacteria were strongly sensitive to the marine bacteria. only two strains (edwardsiella tarda and pseudomonas aerugin ...19883204073
the nucleotide sequence of the argt locus of aeromonas hydrophila. 19883205735
serological comparison between isolated strains of aeromonas salmonicida by agglutination and enzyme-linked immunosorbent assay (elisa). 19883206945
antibiotic sensitivities of aeromonas hydrophila cultured from medicinal leeches.increased use of medicinal leeches (hirudo medicinalis) for the treatment of venous congestion in flaps and replanted parts has coincided with reports of soft tissue infections following leech application. we cultured the gullets of 20 medicinal leeches to re-examine the antibiotic sensitivities of aeromonas hydrophila, the leech enteric organism associated with reported infections. these isolates reflected reported resistance to penicillin and first generation cephalosporins as well as sensitiv ...19883207967
[bacteria reducing chromium in nature and in effluents of industrial plants].bacteria capable of hexavalent chromium (cr6+) reduction can be found in cr6+-containing sewage and sediments of purification tanks of industrial plants. they cannot be detected in water and soil samples containing no chromium compounds. bacteria reducing chromium belong to the genera aeromonas, escherichia, pseudomonas and enterobacter. their activity of cr6+ reduction correlates with the high resistance to the elevated content of this ion in the medium. the fine cell structure of these bacteri ...19883211011
evaluation of several selective media for recovery of aeromonas hydrophila from polluted waters.eleven media were studied for their suitability in the selective isolation of aeromonas hydrophila. preliminary results showed that five of them (inositol-brilliant green-bile salts agar, bile salts-brilliant green agar, rimler-shotts agar, xylose-sodium deoxycholate-citrate agar, and dextrin-fuchsin-sulfite agar) allowed the growth of several microorganisms that are usually present in the same samples in which a. hydrophila is found. six media (ma agar, modified rimler-shotts agar, dnase-toluid ...19883214158
nutritional versatility and growth kinetics of an aeromonas hydrophila strain isolated from drinking water.the nutritional versatility and growth kinetics of aeromonas hydrophila were studied to determine the nature and the growth-promoting properties of organic compounds which may serve as substrates for the growth of this organism in drinking water during treatment and distribution. as an initial screening, a total of 69 different organic compounds were tested at a concentration of 2.5 g/liter as growth substrates for 10 a. hydrophila strains. of these strains, strain m800 attained the highest maxi ...19883214162
enteropathogenicity of aeromonas species isolated from infants: a cohort study.the significance of aeromonas spp. as potential enteric pathogens was evaluated in a cohort of 187 infants aged 3-18 months during a 16-week summer period. aeromonas spp. were isolated from 14 of the 196 (7.1%) diarrhoeal episodes detected and from eight (5.2%) of 153 samples from paired asymptomatic infants. carriage of bacterial enteropathogens excluding aeromonas spp. was detected in a high proportion (23%) of the asymptomatic children. almost all of the seven isolates of aeromonas sobria wer ...19883216131
[toxic properties of lipopolysaccharide and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883216765
[immunogenic and antigenic properties of bacterial cells, lipopolysaccharides and lipids a of aeromonas sobria and non 01 vibrio cholerae]. 19883216766
[which are the most frequently occurring enteropathogenic bacteria in clinical practice?].diarrhea is an increasingly frequent clinical finding in swiss medical practice. one reason is the volume of tourism in countries with different climatic and social circumstances. to obtain an overview of the enteropathogenic bacteria spectrum as a cause of diarrhea, we analyzed 11,163 stool specimens from a representative group of patients throughout switzerland. enteropathogenic bacteria were found in 15.2% of all specimens; 50% were campylobacter jejuni/coli, 35% salmonella spp., 8% aeromonas ...19883217775
carbon substrate assimilation patterns of clinical and environmental strains of aeromonas hydrophila, aeromonas sobria and aeromonas caviae observed with a micromethod.the assimilation of carbon substrates by 103 strains of aeromonas of different origin identified by conventional methods was studied by means of a standardized micromethod containing 147 tests (api system). six distinct groups could be recognized and the discriminating substrates were determined. 3 species of aeromonas can be identified by means of conventional method: a. hydrophila, a. sobria and a. caviae. the method has a number of drawbacks: some media are unreliable, others are difficult to ...19883218392
[the occurrence of bacterial infections in the swamp beaver (myocaster coypus molina, 1782). 3. streptococcal, staphylococcal, aeromonas and actinobacillus infections]. 19883219032
in vitro activity of cefixime, a new oral cephalosporin.the in vitro activities of cefixime and seven comparative oral antimicrobials were studied. mic90s of cefixime were 0.015-1 micrograms/ml for haemophilus influenzae, branhamella catarrhalis, escherichia coli, klebsiella, citrobacter diversus, proteus, providencia, aeromonas hydrophila and streptococcus. mic90s for other enterobacteriaceae were greater than or equal to 2 micrograms/ml; 44% of those strains were inhibited by less than or equal 1 microgram/ml. staphylococcus, psuedomonas aeruginosa ...19883219746
seasonal distribution of aeromonas hydrophila in river water and isolation from river fish.the seasonal distribution of aeromonas hydrophila in water and recovery rate from live river fish was investigated. the highest isolation rates of a. hydrophila occurred in water during the late winter followed by a progressive decline in density during the summer and monsoon seasons. the organism was recovered from fish throughout the period from which it was concluded that they form a reservoir which is unrelated to their density in water. the enterotoxigenicity of some environmental strains w ...19883220801
clinical safety and efficacy of ofloxacin.in this study we report about the efficacy and tolerability of ofloxacin in the treatment of 15 patients with severe and moderately severe infections including osteomyelitis (5), soft tissue infections (5), salmonellosis in aids patients (2), acute or chronic pulmonary infections (2) and mediastinitis (1). the following organisms were isolated in culture specimens: staphylococcus aureus (4), pseudomonas aeruginosa (4), staphylococcus epidermidis (3), serratia marcescens (1), escherichia coli (1) ...19883224401
aeromonas infection following a snake bite: a case report.a twelve year-old thai boy with fulminating aeromonas hydrophila infection of the right arm following a snake bite was reported. he recovered uneventfully after appropriate antimicrobial therapy and surgical intervention.19883227407
correlation of the suicide phenomenon in aeromonas species with virulence and enteropathogenicity.certain strains of mesophilic aeromonads (aeromonas hydrophila, a. sorbria, and a. caviae), when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. because these strains are nonviable after 24 h, this phenomenon is termed suicide. we investigated suicidal strains of aeromonas species as a means of understanding animal virulence and enteropathogenicity. to assess virulence, batches of five white mice were inoculated intraperitoneally with 10(7 ...19883230136
preparation of hemolysin from aeromonas. 19883231101
electrophoretic data in designing strategies for purification and identification of a highly polymorphic bacterial esterase.we have purified a bacterial enzyme, designated esterase m, by tailoring an efficient and rapid strategy with information derived from titration curves of proteins in crude extract. the ph-dependent stability of the enzyme activity observed by titration pattern allowed an acidic ph treatment of extract and a cationic exchange chromatography at ph 4.1. these two steps were followed by an anionic exchange chromatography and a preparative electrophoresis. thus, the enzyme was purified about 2000-fo ...19883235767
[septicemia caused by aeromonas/plesiomonas in adults]. 19883244913
[serological cross reactions of lps and lipid a isolated from aeromonas sobria and non 01 vibrio cholerae]. 19883246916
[characteristics of immunoglobulins specific for lps and lipid a of aeromonas sobria and non 01 vibrio cholerae]. 19883246917
[an epidemic of food poisoning by aeromonas hydrophila]. 19883248456
three-dimensional structure of the crystalline surface layer from aeromonas hydrophila.the three-dimensional (3d) structure of the crystalline surface layer of aeromonas hydrophila has been determined to a resolution of 1.3 nm by crystallographic electron microscopy. the s-layer has tetragonal symmetry, with a unit cell dimension of 12.0 nm and a thickness of 5.6 nm. the 3d reconstruction reveals a distinct domain structure, with one main protein mass at one of the fourfold symmetry axes and a minor part at the other.19883249041
aeromonas hydrophila septicaemia and muscle abscesses associated with immunosuppression.we report a case of septicaemia and muscle abscesses due to aeromonas hydrophila. the patient was immunosuppressed due to hodgkin's disease and treatment with cytotoxic chemotherapy. the abscesses resolved after prolonged antibiotic therapy. organisms that were once considered rarely pathogenic are emerging as increasingly important in causing potentially lethal infections in immunosuppressed patients.19883251226
aeromonas hydrophila cellulitis and wound infections caused by waterborne organisms. 19883257483
beta-lactam resistance in aeromonas spp. caused by inducible beta-lactamases active against penicillins, cephalosporins, and carbapenems.use of cefoperazone in a patient with aeromonas caviae in the respiratory tract selected a mutant that constitutively produced beta-lactamase. this mutant, in contrast to its parental strain with an inducible beta-lactamase, showed enhanced resistance to newer cephalosporins and aztreonam. this observation suggested that species of aeromonas, like those of other genera with inducible beta-lactamases, may pose therapeutic problems associated with the rapid development of multiple beta-lactam resi ...19883264129
effect of recent antibacterial agents against bacteria causing diarrhoea.the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...19883266900
gene probes for the detection of aerolysin in aeromonas spp. 19883269364
proceedings of the second aeromonas/plesiomonas workshop. miami beach, florida, usa, 7 may 1988. 19883269365
characterization of an aeromonas hydrophila strain isolated on a septicemic out-break in a fish-farm of spain.an aeromonas hydrophila strain (ah-3) was isolated from a septicemic out-break on a gold-fish farm near barcelona (spain). on the bases of their virulence and surface characteristics was classified as moderate to weakly virulent.19883271064
aeromonas hydrophila infections in children. 19883272526
the growth of aeromonas hydrophila k144 in ground pork at 5 degrees c.the influence of nacl, ph, atmosphere, and background microflora on the growth and/or survival of aeromonas hydrophila k144 was studied in ground pork held at 5 degrees c. in ground pork, a hydrophila was sensitive to ph values below 6.0 in the form of either a low starting ph in the pork itself or induced by lactic acid bacteria action on added glucose. growth of the organism is inhibited by nacl levels of 3% (w/w) (approx. 4% brine content). a hydrophila grew in vacuum-packaged ground pork; it ...19883275309
surface protein composition of aeromonas hydrophila strains virulent for fish: identification of a surface array protein.the surface protein composition of members of a serogroup of aeromonas hydrophila which exhibit high virulence for fish was examined. treatment of whole cells of representative strain a. hydrophila tf7 with 0.2 m glycine buffer (ph 4.0) resulted in the release of sheets of a tetragonal surface protein array. sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis analysis showed that this sheet material was composed primarily of a protein of apparent molecular weight 52,000 (52k protein) ...19883276660
tigemonam, an oral monobactam.tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ...19883279906
molecular cloning of a phospholipid-cholesterol acyltransferase from aeromonas hydrophila. sequence homologies with lecithin-cholesterol acyltransferase and other lipases.we have determined the nucleotide sequence of a gene encoding aeromonas hydrophila phospholipid-cholesterol acyltransferase, an enzyme which shares many properties with mammalian lecithin:cholesterol acyltransferase. the derived amino acid sequence of the protein contains two regions which are homologous to the proposed active sites and binding sites of the plasma acyltransferase and to similar sequences in other interfacially acting lipolytic enzymes. the amino terminus is preceded by a typical ...19883280033
aeromonas hydrophila gas gangrene: a case report of management with surgery and hyperbaric oxygenation. 19883282228
pneumonia due to aeromonas hydrophila-complex: epidemiologic, clinical, and microbiologic features.review of published data examining the various types of infections produced by strains of the aeromonas hydrophila-complex demonstrates a paucity of information on lower respiratory tract infections due to this organism. although it is rarely cited as a cause of pneumonitis, we have been able to collect epidemiologic, clinical, and microbiologic data on eight patients who have had evidence of aeromonas pneumonia. interestingly, seven of the eight patients were male, and the average age for the g ...19883282317
specific inhibition of aspartase by s-2,3-dicarboxyaziridine.aspartase of escherichia coli was inhibited in a competitive manner by s-2,3-dicarboxyazirdine (dcaz), an antibacterial substance against aeromonas salmonesida. the inhibition constant (ki) was 55 microm, which was as low as less than one tenth that of the km value for the substrate, l-aspartate. in view of the fact that both aspartase and fumarase (j. greenhut et al. (1985) j. biol. chem. 260, 6684-6686) were inhibited by dcaz in competitive manners, common features of the reaction mechanism of ...19883289541
partial purification and characterization of an extracellular proteinase from aeromonas hydrophila strain a4.an extracellular metalloproteinase from aeromonas hydrophila strain a4, isolated from milk, was purified by a factor of 300 by chromatography on deae-cellulose and sephadex g-150. the enzyme had a mol. wt of 43,000 and contained 2 g atom ca/mol. it was active over a ph range 4.8-9.5 and had optimum activity on casein at ph 7.0 with km = 0.17 mm. it was strongly inactivated by metal chelators and the apoenzyme was fully reactivated with ca2+, mn2+ or co2+. heavy metal ions such as ag+, hg2+, fe2+ ...19883290294
critical ionizing groups in aeromonas neutral protease.aeromonas neutral protease possesses two residues critical to its activity. one has a pka of 5.5 in both the free enzyme and the enzyme-substrate complex and must be deprotonated for maximal activity. the other, which ionizes at ph 7.1 in the free enzyme and at ph 7.4 in the enzyme-substrate complex, must be protonated for optimal enzyme action. the protease is reversibly inhibited by aminoacyl hydroxamates, peptides containing a phenylalanyl residue, phosphoryl-l-phenylalanylglycylglycine, and ...19883123480
effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens.in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ...19883127604
hospital-associated infection from leeches. 19883044210
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...19883045003
phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant aeromonas hydrophila from cultured channel catfish (ictalurus punctatus) and their environments.oxytetracycline-resistant (otcr) and tetracycline-resistant (tcr) aeromonas hydrophila were isolated commonly from catfish intestinal contents and the water and sediment of catfish culture ponds, but less frequently in market catfish. isolates demonstrated two resistance phenotypes, tcr otcr and tcs otcr, when plated directly on to macconkey agar containing 30 micrograms of tetracycline or oxytetracycline per ml. tcs otcr isolates expressed tcr after induction by 1 h of growth in tryptic soy bro ...19883046493
mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum.the importance of the genus aeromonas in human disease has recently become better appreciated through the use of improved methodologies for the recovery and identification of aeromonads from biologic specimens and as a result of medical studies aimed at determining the clinical significance of these bacteria when isolated from localized and invasive infections of humans. taxonomic schemes currently allow for the identification of six aeromonas species (five mesophilic and one psychrophilic), fou ...19883055195
the production of a macrophage-activating factor from rainbow trout salmo gairdneri leucocytes.rainbow trout head kidney and blood leucocytes are shown to be capable of secreting a soluble macrophage-activating factor (maf) after stimulation with concanavalin a (con a). the presence of phorbol myristate acetate (pma) as a co-stimulant increased the production of maf. both respiratory burst activity (nitroblue tetrazolium, nbt, reduction and h2o2 production) and bactericidal activity were enhanced after incubation of resident or elicited macrophages with the maf-containing supernatants for ...19883056854
differential susceptibility of salmonid fishes to furunculosis correlates with differential serum enhancement of aeromonas salmonicida extracellular protease activity.rainbow trout, atlantic salmon and brown trout, respectively, exhibit an increasing order of susceptibility to furunculosis. we investigated if this may be due to a differential sensitivity to exotoxins or ability to inhibit the exoprotease which is required by the bacterium to digest protein for growth. differences were slight in susceptibility to exotoxins and serum inhibition of exoprotease at high exotoxin:serum ratios. however, at low ratios brown trout serum markedly enhanced protease acti ...19883059141
aeromonas and plesiomonas as etiological agents. 19883059998
bacterial-induced diarrhoea.in diverse parts of the world, many bacterial strains have been recognised as the causative agents of diarrhoea. great achievements have been made in perceiving the varied mechanisms which explain their intestinal pathogenicity. these achievements include the elucidation of the 5 mechanisms of disease production by escherichia coli, the mechanisms of action of shigella, salmonella, campylobacter and yersinia, new information about antimicrobial-associated colitis caused by clostridium difficile ...19883069445
expression of met-(-1) angiogenin in escherichia coli: conversion to the authentic less than glu-1 protein.a method for obtaining authentic human angiogenin utilizing an escherichia coli recombinant expression system is described. a synthetic gene encoding angiogenin was placed into a vector for direct expression under the control of a modified e. coli trp promoter. the protein was produced by the bacteria in an insoluble form and purified to homogeneity by cation-exchange and reversed-phase hplc following reduction/solubilization and reoxidation. the protein isolated was identified as met-(-1) angio ...19883071185
incidence and biotyping of aeromonas species from the environment.a survey was carried out to establish the incidence rate of aeromonas species in various environmental samples with a view to ascertaining their reservoirs and distinguishing the biotypes. high rates of isolation were obtained from snails and water. this indicates that these samples are likely reservoirs and therefore constitute high sources of human infection. low incidence rates were realised from poultry, cattle and humans, thus suggesting that these samples may be transient hosts. two specie ...19883071728
enzymatic characterization of three aeromonas species using api peptidase, api "osidase," and api esterase test kits.an enzymatic characterization of 16 strains of aeromonas species including a. hydrophila (7), a. sobria (5), and a. caviae (4) was carried out using api peptidase (strips numbered 1, 2, 3, 4, 5, and 6); api esterase and api "osidase" test strips. a total of 89 substrates was used in the assay and included 59 arylamides (aminopeptides), 10 esters, and 20 carbohydrates. all three species were remarkably uniform in their reactivities. nineteen (32%) of the arylamide substrates used were hydrolyzed ...19883072150
aeromonas hydrophila-punctata in two sicilian reservoirs: density of the organisms in relation to physico-chemical and bacteriological parameters. 19883072527
vibrios and aeromonas.there are many similarities in the vibrionaceae that cause human illness in the united states (see table 1). vibrios are characteristically indigenous to marine, estuarine, and brackish environments. they are distributed mainly in gulf of mexico coastal water, and these organisms "bloom" when the water is warm. outbreaks of disease in humans frequently occur in summer, coinciding with multiplication of vibrios in warm water. sporadic cases and small outbreaks of cholera continue to occur in pers ...19883074121
a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...19883076719
epidemiology of aeromonas and plesiomonas diarrhoea. 19883077628
comparison of methods for the induction, propagation and recovery of l-phase variants of aeromonas spp. 19883077629
aeromonas and plesiomonas in the environment: value of differential biotyping of aeromonads. 19883077630
human extraintestinal infections caused by aeromonas species. 19883077631
drug-resistant plasmids from fish pathogens.the epidemiological surveillance of drug-resistant strains of the fish pathogenic bacteria vibrio anguillarum and pasteurella piscicida carrying transferable r plasmids in fish farms is described. the dna structure of r plasmids, and the drug-resistant determinants of r plasmids from the fish pathogens aeromonas hydrophila,aeromonas salmonicida, edwardsiella tarda, v. anguillarum, and p. piscicida are discussed.19883079184
Displaying items 1301 - 1400 of 5519