Publications

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[pathogen distribution in waste water sprinkler irrigation].the spray irrigation with pretreated wastewater was investigated on the sewage farm of the braunschweig sewage utilization association. the emission of airborne bacteria was measured by means of andersen sampler (as), reuter centrifugal sampler (rcs), and sedimentation plates (sp). there was a good correlation of results obtained by parallel measurings of as and rcs. the rcs was more effective by the factor 11.5 than the as sampling airborne microorganisms. however, the as gaves the distribution ...19846377753
bacteria isolated from lymph nodes of california slaughter swine.lymph nodes from 280 slaughter swine from 9 california ranches were examined for the presence of aerobic microflora. genera of interest which were isolated (and percentage of animals from which they were isolated) included salmonella (4.3%), beta-hemolytic streptococci (8.2%), mycobacterium avium-complex (6.4%), corynebacterium pyogenes (0.4%), and aeromonas hydrophila (5.7%). patterns of bacterial isolations from swine herds may be of assistance in predicting herd health problems.19846383150
isolation of aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates.the occurrence of aeromonas spp. in the metropolitan water supply of perth, western australia, australia, was monitored at several sampling points during a period of 1 year. water within the distribution system conformed to international standards for drinking water but contained aeromonas spp. in numbers comparable to those in raw surface water, although this water was free of escherichia coli. coliforms and e. coli were found in raw surface waters, and aeromonas spp. were found in raw water fr ...19846385848
effects of short-term cold storage on recovery of proteases from extracellular products of aeromonas hydrophila.three protease-containing fractions were recovered by gel filtration from concentrated crude extracellular products produced by aeromonas hydrophila grown in a defined medium. the recovery of a heat-stable protease was differentially prevented when the crude preparation was stored for 48 h at -20 degrees c but was unaffected by storage of the crude preparation at either 4 or -70 degrees c. once fractionated, the heat-stable protease appeared to be unaffected by subsequent storage at 4, -20, or - ...19846385854
[sensitivity of diarrhea organisms to nifuroxazide]. 19846386203
evaluation of a two-hour method for screening pathogens from stool specimens.the 2-h stool-screening test (sst) (api system s.a., montalieu-vercieu, france) was used to screen 231 organisms yielding suspicious colonies on stool differential agars for potential pathogens. all 54 salmonellae yielded correct screens. of 14 shigellas, 9 keyed as possible shigella spp.-yersinia enterocolitica-rare salmonella spp. (sys), and 5 (all shigella sonnei) keyed as possible s. sonnei-y. enterocolitica-arizona spp. (sya). three arizona strains were identified as probable salmonella spp ...19846386849
aeromonas. 19846389407
aspirin in acute gastroenteritis: a clinical and microbiological study.soluble aspirin given by mouth in divided dosage decreased intestinal fluid loss in infants and young children with acute gastroenteritis. the treated group had significantly less diarrhea, which ceased earlier and needed less intravenous therapy, than a randomly selected control group given an indistinguishable placebo. this effect of aspirin occurred with diarrhea caused by salmonella, aeromonas, escherichia coli producing heat-labile toxin, and rotavirus, but not with diarrhea associated with ...19846389815
aeromonas hydrophila and plesiomonas shigelloides as causes of intestinal infections.infections due to aeromonas hydrophila and plesiomonas shigelloides in immuno-compromised hosts have been well documented, but the role of these organisms in causing gastrointestinal disease in healthy persons is controversial. despite difficulties in characterizing the exotoxins produced by aeromonas species, there is accumulating evidence that these bacteria are capable of causing usually mild, self-limited diarrheal disease in previously healthy adults. some pediatric patients may have protra ...19846390638
prophylactic doxycycline for travelers' diarrhea in thailand. further supportive evidence of aeromonas hydrophila as an enteric pathogen.a randomized double-blind study to determine the efficacy of a three-week course of doxycycline (100 mg daily) in preventing travelers' diarrhea was performed in 1980 among 63 united states peace corps volunteers during their first five weeks in thailand, an area where doxycycline-resistant enterotoxigenic escherichia coli are known to be common. eight (24%) of 33 volunteers taking placebo and 3 (10%) of 30 taking doxycycline developed travelers' diarrhea for a calculated protection of 59%, but ...19846391157
[demonstration of a proteolytic activity on intracellular origin in aeromonas hydrophila lp 50].aeromonas hydrophila lp 50, isolated from packaged pasteurized milk, was grown in glucose-polypeptone medium at 30 degrees c. the proteolytic activity of a. hydrophila lp 50, optimum at the stationary phase of growth, is attributed to extracellular or membrane protease; no intracellular proteolytic activity was shown.19846391644
collaborative evaluation of the abbott avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli.the capability of the abbott avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. the avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded abbott bacterial identification cartridge plus the results of a manual oxidase test. the species included in the avantage data base are acinetobacter anitratus, acinetobacter iwoffi, aeromon ...19846392323
[evaluation of results in the identification of various aerobic gram-negative bacteria in selected bacteriological laboratories in poland]. 19846392790
the impact of terrestrial and estuarial factors on the density of environmental bacterial (vibrionaceae) and faecal coliforms in coastal water.a study of the densities of faecal coliforms, vibrio anguillarum, vibrio alginolyticus and aeromonas hydrophila in danish bathing areas was carried out over the period may-october, 1981 with special reference to specific terrestrial and estuarial determinants. significant correlations were found between v. anguillarum and v. alginolyticus (r = 0.57) and a. hydrophila and faecal coliforms (r = 0.43). the densities of the vibrios were highly influenced by temperature and distance from pollution so ...19846395571
[severe aeromonas hydrophila infections. apropos of 2 cases].the authors describe two personal cases of severe aeromonas hydrophila infection. they review the symptomatology and treatment of such cases and insist on the unusual features in their patients.19846322344
[aeromonas hydrophila in ivory coast epidemiologic and ecologic aspects and its enteropathogenic role].a research based on the analysis of 479 coprocultures , allow the isolation of 22 strains of aeromonas hydrophila. the epidemiological aspects, the contamination processes through water, food... and the enteropathogenic role of these aeromonas are discussed. these bacteria seen responsible of digestive disturbances from simple gastric discomfort (nausea, light abdominal pain...) to the actual choleriform syndrome. the isolation of 7 strains from the environment (water from lagune , wells; lagune ...19846327100
[an aeromonas hydrophila choleriform syndrome. apropos of a case from the ivory coast].an observation of a case of choleriform syndrome of the aeromonas hydrophila subspecies anaerogenes biotype ii is reported. in a country dealing with a severe comma bacillus under endemo -sporadic from, the case report here could be wrongly diagnosed as cholera.19846327101
comparative study of the nature and biological activities of bacterial enterotoxins.it is apparent that there are considerable similarities between many of the enterotoxins produced by enteric pathogens. although the effect of most of these toxins is restricted to the intestine in vivo, many cells are also sensitive to intoxication in vitro. the resultant in-vitro biochemical changes may have no pathological significance but serve to underline the central role of cyclic nucleotides in cellular fluid regulation. the biological activity of these enterotoxins is the result of inte ...19846327986
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...19846331331
resistance of aeromonas hydrophila to beta-lactam antibiotics.the activity of some beta-lactam antibiotics upon 20 strains of aeromonas hydrophila and some properties of their beta-lactamases have been studied. high degree of resistance to benzylpenicillin and lower resistance to ampicillin and cephaloridine was observed. clavulanic acid showed the highest activity. when clavulanic acid was assayed at subinhibitory concentrations in association with ampicillin. mics of the latter decreased to two- to eight-fold. all strains produced inducible beta-lactamas ...19846335147
[spoilage microorganisms encountered in ultra-high temperature processed milk].40 strains of aerobic or facultative anaerobic microorganisms were isolated from a total of 37 spoiled ultra high temperature processed milk. 13 of them were identified as the genus bacillus. they were 6 b. cereus, 5 b. licheniformis, 1 b. brevis and 1 b. pumilus. the other 27 strains were nonsporeforming microorganisms, which included 5 yeasts, 2 pseudomonas sp., 3 streptococcus sp., 12 lactobacillus sp., 1 shigella sp., 1 aeromonas sp. and 3 micrococcus sp. results indicate that the spoilage o ...19846468029
[distribution of enterobacteria in soil following irrigation with waste water].the filtration of wastewater after irrigation by sandy soil in the area of the braunschweig sewage utilization association was investigated. for that purpose, the concentrations of gram-negative aerobe rods were determined in soil samples of 15 cm, 30 cm, 45 cm, 60 cm, 75 cm, 90 cm, 105 cm, 120 cm, 135 cm, and 150 cm depth immediately after an irrigation of about 50 mm wastewater during the vegetation (3 series), the vegetationless period (6 series), after one week (3 series) and after two weeks ...19846475379
[aeromonas hydrophila septicemia: 3 cases, 1 with mediastinitis].three cases of aeromonas hydrophila septicaemia are reported. the first case concerned a 51 years old patient suffering from drowning who had previously been in good health and who died after a few days of refractory hypoxaemia. the second case was an 8 years old child with trisomy 21 operated for a congenital cardiac defect. the portal of entry was a catheter and the septicaemia was complicated by a. hydrophila mediastinitis, a complication which has not been described previously. the third cas ...19846476665
difference in the extracellular products of two strains of aeromonas hydrophila virulent and weakly virulent for fish.in this study we first compared the toxigenic profile of aeromonas hydrophila strains virulent and weakly virulent for rainbow trout. the separation of the toxic factor for fish is also described. both strains produced hemolytic, enterotoxic, and dermonecrotic activities; the weakly virulent strain produced 20 times more hemolysin. only the cell-free supernatant of the virulent strain produced a toxic factor for fish. after passage on sephacryl s-200, the toxic factor for fish was separated from ...19846478313
nucleotide base sequence of vibrionaceae 5 s rrna.nucleotide base sequences of 5 s rrnas isolated from vibrio vulnificus, vibrio anguillarum, and aeromonas hydrophila were determined. comparisons among these and sequences of 5 s rrnas from other species of vibrionaceae provide information useful in the evaluation of the evolution of bacterial species.19846479333
purification and some properties of aeromonas hydrophila hemolysin.a hemolysin produced by a strain of aeromonas hydrophila isolated from a patient with diarrhea was purified by acid precipitation and quarternary aminoethyl-sephadex chromatography. the molecular weight of the hemolysin was estimated at 50,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and at 48,000 by sephadex g-100 gel filtration. in polyacrylamide gel electrophoresis at ph 4.0 and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hemolysin migrated as a single b ...19846480102
thromboxane interaction with cardiopulmonary dysfunction in graded bacterial sepsis.the relationship between plasma levels of thromboxane a2, radioimmunoassayed as thromboxane b2 (txb), and cardiopulmonary dysfunction in graded bacterial sepsis was investigated. five adult female pigs under anesthesia were intubated and allowed to breathe room air spontaneously. femoral arterial, venous, and pulmonary artery catheters were inserted. after a 60-minute control period aeromonas hydrophila (1.0 x 10(9)/ml) was infused intravenously at 0.2 ml/kg/hr, gradually increasing to 4.0 ml/kg ...19846481830
isolation of aeromonas spp. from an unchlorinated domestic water supply.the recovery of aeromonas spp. from the unchlorinated water supply for a western australian city of 21,000 people was monitored at several sampling points during a period of 1 year. membrane filtration techniques were used to count colonies of aeromonas spp., coliforms, and escherichia coli in water sampled before entry to service reservoirs, during storage in service reservoirs, and in distribution systems. aeromonas spp. were identified by subculture on blood agar with ampicillin, oxidase test ...19846486783
the occurrence of aeromonas hydrophila in avian diagnostic submissions.twenty isolations of aeromonas hydrophila were made from 15 species of free-living, commercial, and companion birds submitted for routine diagnostic postmortem examination. seventy percent of the isolations were made from november through march during the 25-month survey period.19846487200
structure of the lipopolysaccharide core isolated from a human strain of aeromonas hydrophila.lipopolysaccharide was isolated from the cell-walls of a human strain of aeromonas hydrophila by the aqueous phenol method in 0.58% yield (based on dry weight of bacteria). the lipopolysaccharide consisted of sr-polysaccharide, core-oligosaccharide and lipid a; there was no o-specific polysaccharide. the core had the composition d-galactose, d-glucose, d-glycero-d-manno-heptose, l-glycero-d-manno-heptose and d-glucosamine in a molar ratio of 1:1:2:4:1. glucosamine was linked to an l-glycero-d-ma ...19846489347
action of a microbial glycerophospholipid:cholesterol acyltransferase on plasma from normal and lcat-deficient subjects.the action of a bacterial acyltransferase similar in overall reaction mechanism to the plasma enzyme lecithin:cholesterol acyltransferase (lcat) has been studied using normal plasma and lipoproteins and plasma from lcat-deficient patients. the microbial enzyme (gcat) catalyzed acyl transfer using phosphatidylcholine and cholesterol in all of the lipoprotein fractions, presumably because it has no apolipoprotein cofactor. in addition, the enzyme was capable of hydrolyzing cholesteryl ester in lip ...19846491538
furunculosis in rainbow trout (salmo gairdneri) raised in sea water.ulcerative skin lesions were encountered in rainbow trout raised in sea water by a commercial concern in the western cape, south africa. grossly, the lesions resembled furunculosis but, histopathologically, they differed from typical furunculosis in that bacterial colonies were rarely found in the organs, and also the kidneys and spleens were minimally involved. the causative organism was identified as an achromogenic aeromonas salmonicida that shared characteristics with all 3 subspecies, salmo ...19846493730
aeromonas toxins. 19846494019
aeromonas infection in an infant with aplastic anemia. 19846494020
cloning of enterotoxin gene from aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.culture filtrates of two aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic, cytotoxic, and hemolytic activities. modest heat treatment of the filtrates inactivated the cytotoxic and cytolytic activities, but not the enterotoxic activity. the construction of cosmid gene banks in escherichia coli of dna from both a. hydrophila strains demonstrated that the determinants of the three act ...19846500697
aeromonas hydrophila colitis in a child.a 5-year-old girl presented with chronic bloody diarrhea. evaluation including sigmoidoscopy, rectal biopsy, and barium enema was consistent with the diagnosis of ulcerative colitis. culture of the stool grew aeromonas hydrophila. a. hydrophila colitis may be more common than presently realized.19846502383
media for isolation of aeromonas spp. from faeces.five solid media were evaluated for isolation of aeromonas spp. from faeces: desoxycholate citrate agar (dca), macconkey's agar (mac), xylose-desoxycholate-citrate agar (xdca), rogol's medium (rog), which contained ampicillin 20 mg/l and p-nitrophenyl-glycerine 25 mg/l as inhibitors, and blood agar (ba) with ampicillin 10 mg/l. false negative oxidase tests limited the usefulness of dca and mac and, although the use of xdca avoided the problem of fermentation of lactose, some aeromonas spp. faile ...19846502678
production of cholera-like enterotoxin by aeromonas hydrophila.a total of 249 strains of mesophilic aeromonas including 179 a. hydrophila and 70 a. caviae were tested for production of cholera-like enterotoxin by reversed passive latex agglutination (rpla) assay. a cholera-like enterotoxin neutralized with cholera antitoxin was demonstrated in the culture filtrates from eight (4.5%) of the 179 a. hydrophila strains, while none of a. caviae strains revealed the enterotoxin production in the test. production of the cholera-like enterotoxin in the eight strain ...19846503026
isolation of enterotoxigenic, hemolytic, and antibiotic-resistant aeromonas hydrophila strains from infected fish in bangladesh.strains of aeromonas hydrophila isolated from skin infections of common freshwater fish in bangladesh were tested for enterotoxin production, hemolysin production, and any correlation between these two activities. we also tested the resistance patterns of a. hydrophila to different drugs, especially in relation to ampicillin. the a. hydrophila strains produced an enterotoxin that was related to their beta-hemolytic activities. production of beta-hemolysin may thus be an indicator of enterotoxici ...19846508295
plasmid coding for transferable drug resistance in bacteria isolated from cultured rainbow trout.the occurrence of drug resistance and plasmid-mediated transferability was investigated in 170 strains belonging to eight bacterial groups isolated from cultured rainbow trout. it was found that 87.6% of the strains were resistant to at least one drug, with the highest percentages of resistance being detected for ampicillin (54.7%), sulfadiazine (46.5%), nitrofurantoin (38.2%), and chloramphenicol (37.0%). six enterobacteria, two vibrio, and one aeromonas isolate transferred resistance factors t ...19846508296
aeromonas septicemia with shock in children with thalassemias. 19846512449
value of blood agar for primary plating and clinical implication of simultaneous isolation of aeromonas hydrophila and aeromonas caviae from a patient with gastroenteritis.the simultaneous recovery of aeromonas hydrophila and aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of a. hydrophila. in vitro phenotypic tests support the conclusion that only the a. hydrophila isolate was clinically significant.19846520231
surface layer virulence a-proteins from aeromonas salmonicida strains.superficial surface layer proteins (a-proteins) were present on diverse isolates of aeromonas salmonicida which differed both physiologically and in pathogenesis. three of these proteins were purified directly from the surface of whole cells or from outer membrane preparations. these a-proteins were unusually hydrophobic (45-47%) and of similar but not identical molecular mass (49, 50, and 51 kdaltons). they were nearly identical in amino acid composition and were highly conserved, but not ident ...19846525563
phylogenetic relationships among vibrio anguillarum plasmids.results of restriction endonuclease analysis and southern blot hybridization suggest that the r-plasmids from vibrio anguillarum strains isolated in japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. molecular cloning experiments allowed identification of specific restriction endonuclease fragments carrying the genes for either cmr or tcr as the common sequences between some of these groups of r-plasmids. the cmr region fr ...19846528000
aeromonas hydrophilia tenosynovitis in an immunocompromised host. 19846536592
o-serogrouping scheme for mesophilic aeromonas strains.the o-antigens of 307 strains of mesophilic aeromonas including 227 a. hydrophila and 80 a. caviae were studied and 44 o-serogroups defined among them. the presence of heat-labile masked antigen, which inhibits o-agglutination, was observed in some strains. as all the o-antisera prepared with these mesophilic aeromonas strains contained some r-antibody, all diagnostic o-antisera must be absorbed with r-organisms before use. some of the o-antigens were found to be identical or closely related to ...19846536785
[aeromonas hydrophila: epidemiological study in the area around rome]. 19846536924
brief report: bacteriological sampling of telephones and other hospital staff hand-contact objects.in an acute-care general hospital, 114 telephones, intercoms, dictaphones, and bedpan flusher handles were sampled in patient-care areas for type of bacterial contamination. nine of these (7%) demonstrated potentially pathogenic bacteria including klebsiella, enterobacter, pseudomonas and aeromonas. inanimate, environmental, staff hand-contact objects were only lightly contaminated, did not represent a significant reservoir of gram-negative organisms, and therefore, would be unlikely to be a veh ...19846568216
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...19846427786
vaccination of fish: a practical view.commercial vaccines giving protection against three important bacterial fish diseases (enteric redmouth, furunculosis and vibriosis) became available in the united kingdom in 1983. the degree of protection obtained and the cost effectiveness vary according to the method of presentation (injection, dipping or spraying and oral dosing). these methods and the optimum time for vaccination are described.19846431685
aeromonas spp in travellers' diarrhoea. 19846434027
aeromonas spp in diarrhoea. 19846437556
comparative in vitro activities of apalcillin and piperacillin against gram-negative bacilli.the susceptibilities of 317 gram-negative bacilli to apalcillin and piperacillin were determined by standardized microdilution and disk diffusion tests. the respective percentages of strains susceptible to less than or equal to 64 micrograms of apalcillin and piperacillin per ml were as follows: members of the family enterobacteriaceae, 90 and 88%; randomly selected pseudomonas aeruginosa, 100 and 100%; multi-drug-resistant p. aeruginosa, 100 and 75%; and other nonfermenters and aeromonas hydrop ...19846440476
an unusual source of hospital wound infection. 19846440623
methods for the isolation of aeromonas hydrophila and plesiomonas shigelloides from faeces.two solid selective media, xylose deoxycholate citrate agar (xdca) and bile salts brilliant green agar (bbg) and an enrichment broth-alkaline peptone water, were evaluated for the isolation of aeromonas hydrophila and plesiomonas shigelloides. alkaline peptone water and xdca are useful for recovery of aeromonas but not plesiomonas, whereas bbg is satisfactory for both organisms.19846368683
aeromonas hydrophila: a treatable cause of diarrhea. 19846607454
hemagglutination patterns of aeromonas spp. in relation to biotype and source.aeromonas spp. show patterns of hemagglutination with human group o cells in the presence of fucose, galactose, and mannose. these patterns are related to biotype as well as to the source of isolates. there was good correlation between hemagglutination pattern and the presence of diarrhea among strains isolated in western australia, which was the only source with adequate data for classification of children with an without diarrhea. most of the environmental and other nonfecal isolates produced ...19846690466
biotyping of aeromonas isolates as a correlate to delineating a species-associated disease spectrum.a group of 147 aeromonas isolates from diverse clinical and environmental sources was subjected to the biotyping scheme of popoff and veron. of the 147 isolates biotyped, 137 (93%) could be identified, with aeromonas hydrophila predominating (48%) and equal percentages (25 to 27%) of the other two species (aeromonas sobria and aeromonas caviae). a number of additional biochemical properties were found to be significantly associated with one or more of these three species. these included lysine d ...19846690467
bacterial interference with coliform colony sheen production on membrane filters.the membrane filter (mf) method for detection and enumeration of coliform bacteria in drinking water requires that the coliforms both grow and produce a green metallic sheen when the filter is incubated on modified endo medium at 35 degrees c for 22 h. large numbers of noncoliform bacteria, which are enumerated by the standard plate count (spc) technique, can interfere with the detection of coliforms on mf. this paper presents quantitative evidence from laboratory experiments on the interference ...19846696423
enterotoxin production in relation to taxonomic grouping and source of isolation of aeromonas species.a total of 19 of 20 (95%) strains of aeromonas hydrophila biovar hydrophila and 16 of 17 (94%) strains of aeromonas sobria isolated from a variety of clinical and environmental sources were found to be enterotoxin positive. only 2 of 18 (11%) a. hydrophila biovar anaerogenes and 2 of 13 (15%) unidentified aeromonas strains from a similar variety of sources produced enterotoxin. no association was apparent between the source of isolation, in particular diarrheal stools, and enterotoxigenicity; 41 ...19846699147
[infections caused by aeromonas hydrophila]. 19846700763
observations of bacterial diseases of captive snakes in finland.eighteen captive, nonpoisonous snakes which suffered from bacterial infections were studied. the most usual bacteria found were gram-negative rods; especially pseudomonas aeruginosa, aeromonas hydrophila and proteus spp. the most common disorders caused by them were stomatitis, septicaemia, bronchopneumonia and abscesses. the sensitivities of ten isolated bacteria to antimicrobial drugs were tested. neomycin and gentamicin seemed to be effective, especially against p. aeruginosa.19846728671
aeromonas septicemia in infants and children.our experience at the ramathibodi hospital with 20 infants and children who had aeromonas septicemia is reviewed. their ages were from 1 day to 14 years. eighteen patients had underlying diseases: leukemia, 5; aplastic anemia, 4; cirrhosis, 2; thalassemia/hemoglobinopathy, 3; renal failure, 1; ileal perforation, 1; marasmus, 1; and cavernous hemangioma with thrombocytopenia, 1. blood cultures yielded aeromonas hydrophila in all patients, and four patients had polymicrobial bacteremia. fifteen ep ...19846728702
aeromonas hydrophila isolated from polyarthritis in a calf. 19846732673
aeromonas hydrophila in livestock: incidence, biochemical characteristics and antibiotic susceptibility.faecal samples from 110 horses, 115 pigs, 111 sheep and 123 cows were examined for the presence of aeromonas hydrophila, which was also sought in the available drinking water. the overall faecal rate was 11.8%, but significantly more bovine than other samples were found to be positive. there was significant association between the isolation of a. hydrophila from all animal faeces and its presence in drinking water, but this was not found when individual animal groups were analysed separately. an ...19846736644
septicemia due to aeromonas hydrophila. 19846736824
aeromonas hydrophila gastroenteritis in tuscany children. 19846739332
biotyping and virulence factors in clinical and environmental isolates of aeromonas species.biochemical characteristics and virulence factors were compared in 147 aeromonas spp. isolated from patients with diarrhea and in 94 strains isolated from metropolitan water supplies in the same area during the same period. fermentation of arabinose occurred with 58.5% of the environmental strains and 15% of the clinical isolates; 39.4% of the strains from water and 6.8% of the fecal isolates fermented salicin. the frequency of esculin hydrolysis was the same in both groups. ninety-one percent o ...19846742829
severe cellulitis due to aeromonas hydrophilia following immersion injury. 19846745473
use of non-carbonated soft drinks to provide safe drinking water.non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ...19852409896
eosinophilic granular cells (egc) and histamine responses to aeromonas salmonicida toxins in rainbow trout.the function of eosinophilic granular cells (egcs) in salmonids is unknown. in a previous study of the pathogenesis of a. salmonicida, injection of crude exotoxins into rainbow trout were shown to reproduce the lesions associated with furunculosis and an accompanying lesion, the dispersion and degranulation of egcs in the intestinal wall was reported. the present study investigated this phenomenon in relationship to the histamine content of the gut. in fish injected i/p with a. salmonicida exoto ...19852410306
electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of aeromonas hydrophila.sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the lipopolysaccharides isolated from strains of aeromonas hydrophila which exhibit virulence for fish and which autoaggregate during growth in static broth culture. the lipopolysaccharides contained o-polysaccharide chains of homogeneous chain length. two of the strains produced a surface protein array, and immunofluorescence and phage-binding studies revealed that a number of these o-polysaccharide chains of homogene ...19852413008
polymicrobial aetiology of travellers' diarrhoea.of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...19852857430
aeromonas hydrophila infection in the hand. 19852857697
aeromonas hydrophila in chlorinated water supplies.methods for the isolation and enumeration of aeromonas hydrophila in water supplies are described. examination of 286 chlorinated samples collected between july and october showed that 19% of otherwise uncontaminated waters contained aerom. hydrophila, rising to 71% of those from which escherichia coli was isolated. the isolation rate during january and february was only 7% for all samples. on the basis of these results, tap water is a likely source of the aeromonads found in human intestinal fl ...19852859326
the slow, tight binding of bestatin and amastatin to aminopeptidases.bestatin reversibly inhibits aeromonas aminopeptidase (ec 3.4.11.10) in a process that is remarkable for its unusual degree of time dependence. the binding of bestatin by both aeromonas aminopeptidase and cytosolic leucine aminopeptidase (ec 3.4.11.1) is slow and tight, with ki values (determined from rate constants) of 1.8 x 10(-8) and 5.8 x 10(-10) m, respectively. in contrast, microsomal aminopeptidase (ec 3.4.11.2) binds bestatin in a rapidly reversible process with a ki value of 1.4 x 10(-6 ...19852865258
effect of an activated sludge wastewater treatment plant on ambient air densities of aerosols containing bacteria and viruses.bacteria- and virus-containing aerosols were studied during the late summer and fall seasons in a midwestern suburb of the united states before and during the start-up and operation of an unenclosed activated sludge wastewater treatment plant. the study showed that the air in this suburban area contained low-level densities of indicator microorganisms. after the plant began operating, the densities of total aerobic bacteria-containing particles, standard plate count bacteria, total coliforms, fe ...19852988442
synthesis, export, and assembly of aeromonas salmonicida a-layer analyzed by transposon mutagenesis.suicide plasmid pjb4ji, containing transposon tn5 and phage mu, was introduced into aeromonas salmonicida 449 which produces a surface protein array known as the a-layer. kanamycin-resistant exconjugants of 449 with altered ability to produce the a-layer were selected by virtue of their altered colonial morphology and color on medium containing the dye congo red. analysis of culture supernatants, periplasmic shock fluid, outer membranes, and whole-cell lysates by sodium dodecyl sulfate-polyacryl ...19852993248
molecular cloning and expression of a xylanase gene of alkalophilic aeromonas sp. no. 212 in escherichia coli.a gene coding for a xylanase activity of alkalophilic aeromonas sp. no. 212 (atcc 31085) was cloned in escherichia coli hb101 with pbr322. plasmid pax1 was isolated from transformants producing xylanase, and the xylanase gene was located in a 6.0 kb hind iii fragment. the pax1-encoded xylanase activity in e. coli hb101 was about 80 times higher than that of xylanase l in alkalophilic aeromonas sp. no. 212. about 40% of the enzyme activity was observed in the periplasmic space of e. coli hb101. t ...19852999298
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...19853000920
nuclease s1 analysis of eubacterial 5s rrna secondary structure.single-strand-specific nuclease s1 was employed as a structural probe to confirm locations of unpaired nucleotide bases in 5s rrnas purified from prokaryotic species of rrna superfamily i. limited nuclease s1 digests of 3'- and 5'-end-labeled [32p]5s rrnas were electrophoresed in parallel with reference endoribonuclease digests on thin sequencing gels. nuclease s1 primary hydrolysis patterns were comparable for 5s rrnas prepared from all 11 species examined in this study. the locations of base-p ...19853001324
[aquatic contamination of wounds by aeromonas sobria after bathing in the river]. 19853160041
comparative electrophoretic profiles of esterases, and of glutamate, lactate and malate dehydrogenases, from aeromonas hydrophila, a. caviae and a. sobria.esterases, and glutamate, lactate and malate dehydrogenases of 64 aeromonas hydrophila, a. caviae and a. sobria strains, were analysed by polyacrylamide agarose gel electrophoresis and by thin layer isoelectrofocusing. on the basis of the isoelectric points of malate dehydrogenase from the three species and the mobility of lactate dehydrogenase from a. sobria, 8 species specific zymotypes were defined: three for a. hydrophila strains, three for a. caviae strains and two for a. sobria strains. th ...19853831235
aeromonas hydrophila hand infection complicating an open rolando fracture: a case report. 19853831733
purpura fulminans produced by aeromonas hydrophila: a case report.a case of purpura fulminans produced by aeromonas hydrophila in a twelve year-old thai boy was reported. the patient also had aplastic anemia which facilitated the severity of disease. he died despite appropriate antimicrobial therapy.19853835694
[gram-negative bacteria resistant to antibiotics in foods].from 154 food samples, including vegetables (lettuce), milk and meals served at school it was possible to isolate and identify 400 gram negative bacilli distributed among 339 enteric bacteria (escherichia, shigella, citrobacter, klebsiella, enterobacter, serratia and proteus) and other 61 non enteric bacilli (acinetobacter, flavobacterium, aeromonas and pseudomonas). submitting this cultures to the drugs sulfadiazine (su), streptomycin (sm), tetracycline (tc), chloramphenicol (cm), kanamycin (km ...19853837834
acute cholecystitis due to aeromonas hydrophila. 19853864036
in-vitro activity of ofloxacin, a quinolone carboxylic acid compared to other quinolones and other antimicrobial agents.ofloxacin is a new quinolone carboxylic acid compound. its activity against 900 bacterial isolates was determined. it inhibited 90% of escherichia coli, klebsiella sp., aeromonas hydrophila, salmonella spp., shigella spp., citrobacter spp., enterobacter spp., morganella morganii, proteus mirabilis, yersinia enterocolitica at less than or equal to 0.8 mg/l. branhamella catarrhalis, haemophilus sp., neisseria sp. were inhibited by less than or equal to 0.1 mg/l. pseudomonas aeruginosa and other ps ...19853865923
porphyrin binding by the surface array virulence protein of aeromonas salmonicida.congo red binding by virulent a-layer-containing (a+) and avirulent a-layer-deficient (a-) strains of aeromonas salmonicida was examined. congo red binding to a+ cells was enhanced by salt and thus hydrophobically driven, but at low congo red concentrations binding was salt independent. congo red was bound by a+ cells by a kinetically distinct mechanism (kd, 0.25 microm) which was absent in a- isogenic strains. purified a-layer protein ("a protein") protein a also bound congo red with similar af ...19853877720
yersinia enterocolitica infection in children.the role of yersinia enterocolitica as a human pathogen has been documented in publications from over 30 countries, and y. enterocolitica has been recognized increasingly to cause gastrointestinal disease in children. in 1979, an australian survey yielded only three isolates of y. enterocolitica from 3298 faecal specimens obtained from adults. we screened all stool specimens received during a 22-month period for yersinia by means of a recently developed selective agar medium. y. enterocolitica w ...19853877859
disease patterns in the detroit zoo: a study of the avian population from 1973 through 1983.a retrospective study was conducted to evaluate disease patterns in birds at the detroit zoo from 1973 through 1983. data were derived from the zoo's medical and animal census records; the mean (+/- sd) population of birds during the study period was 469 +/- 42. overall annual morbidity rates were 12.5% to 21.5%, with spring months having the highest morbidity rates. annual mortality rates were 3.1% to 15.2%; 23.9% of the deaths were caused by microbial agents (particularly erysipelothrix rhusio ...19853878351
disease patterns in the detroit zoo: a study of reptilian and amphibian populations from 1973 through 1983.a retrospective study was conducted to determine disease patterns in reptilian and amphibian populations at the detroit zoo from 1973 through 1983. in the reptilian population (mean +/- sd = 285.2 +/- 28), overall annual mortality rates were 1% to 40%. mortality rates were highest in the fall months (20%) and lowest in the winter months (6%). the most frequently affected reptiles were iguana (iguana iguana), reticulated python (python reticulatus), rattlesnakes (crotalus spp), common boa (constr ...19853878352
identification of gram-negative bacilli using the autobac idx.the autobac idx is a new system for the rapid identification of clinically significant members of the enterobacteriaceae and aeromonas, acinetobacter, alcaligenes, flavobacterium, moraxella, and pseudomonas species. the use of 18 differentially inhibitory compounds such as dyes and antibiotics along with a computerized algorithm based on a multivariate analysis provides the basis for the identification of 30 different groups of gram-negative bacilli. required preliminary tests include observatio ...19853881212
a case report: aeromonas sobria gastroenteritis in an adult.aeromonas sobria, a member of the aeromonas hydrophila group, recently has been recognized as a gastrointestinal pathogen in adults. there have been a few documented cases, and these have come from far eastern countries, namely india, thailand, and indonesia. the authors have isolated a strain of a. sobria from a 62-year-old male patient suffering from diarrheal disease. he had no history of travel outside of the united states for several decades. he presented with a severe diarrheal disease tha ...19853883745
loss of virulence in a protease-deficient mutant of aeromonas salmonicida.the importance of extracellular protease production by aeromonas salmonicida, the bacterial pathogen of fish furunculosis, was investigated with four virulent strains (which were autoagglutinative, hemagglutinative, resistant to fish serum, adhesive to fish tissue culture, protease positive, hemolysin positive, and leukocytolysin positive) and three avirulent strains (which were nonagglutinative, nonhemagglutinative, sensitive to serum, nonadhesive, protease positive, hemolysin positive, and leu ...19853884507
biochemical and exoenzymatic properties of aeromonas species.one hundred twenty-seven isolates of aeromonas comprising the three currently recognizable species (a. hydrophila, a. sobria, and a. caviae) were evaluated for biochemical and exoenzymatic properties. aeromonas species were generally (greater than 90%) characterized as gram-negative fermentative rods that were oxidase-, catalase-, and beta-galactosidase-positive, produced arginine dihydrolase, and failed to decarboxylate ornithine. more than 95% of all isolates tested failed to grow on 6.5% salt ...19853888508
a comparative study of enterotoxigenic escherichia coli, shigella, aeromonas, and vibrio as etiologies of diarrhea in northeastern thailand.the incidence of enterotoxigenic escherichia coli (etec), shigella, aeromonas, and vibrio was determined in patients with diarrhea seen at a hospital in northeastern thailand, and compared with the incidence of these bacteria in household contacts and their neighbors. etec was identified in 17%, shigella in 9%, aeromonas in 9%, v. parahaemolyticus in 5%, and non-01 v. cholerae in 2% of 299 patients with diarrhea. these five species of bacteria were isolated more often from patients with diarrhea ...19853890578
genetic and biochemical properties of aer-1, a novel carbenicillin-hydrolyzing beta-lactamase from aeromonas hydrophila.a novel carbenicillin-hydrolyzing beta-lactamase has been discovered in a blood isolate of aeromonas hydrophila. the enzyme resembles plasmid-determined carbenicillinases in substrate profile but differs in isoelectric point (pi 5.9) and molecular weight (22,000) and has been termed aer-1. no evidence for a plasmid location could be obtained in a. hydrophila, but the aer-1 gene and resistance to chloramphenicol, streptomycin, and sulfonamide could be transferred by mobilization with incp plasmid ...19853890725
activation of the hole-forming toxin aerolysin by extracellular processing.a precursor-product relationship between aerolysin and a protein with a higher molecular weight was observed in culture supernatants of aeromonas hydrophila. the larger protein was isolated by ammonium sulfate precipitation and ion-exchange and hydroxyapatite chromatography and compared with purified aerolysin. it was at least 250 times less hemolytic than aerolysin. both proteins had the same amino acid sequence at the amino terminus. cyanogen bromide fragments obtained from the two were identi ...19853891735
etiology and epidemiology of diarrheal diseases in the united states.accurate data on the frequency of acute diarrheal illness and the distribution of pathogens are not available for several reasons, including the facts that only a small fraction of cases come to the attention of physicians and that available diagnostic tests establish an etiology in only about half of these. in a survey of three groups of patients in a community (upper-middle class and lower class outpatients and hospitalized infants), a possible cause was found in fewer than 20 percent of outpa ...19853893118
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