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atypical furunculosis vaccines for atlantic cod (gadus morhua); vaccine efficacy and antibody responses.atypical furunculosis caused by atypical aeromonas salmonicida, is an emerging problem in farming of atlantic cod (gadus morhua) in norway, and vaccines are needed. atypical a. salmonicida comprises a heterogeneous group of bacteria differing in surface antigens such as the a-layer protein and lipopolysaccharides (lps). except for one of the experimental oil-adjuvanted whole cell vaccines based on various isolates they all resulted in moderate protection. no clear correlation between vaccine eff ...200818951938
oxytetracycline treatment reduces bacterial diversity of intestinal microbiota of atlantic salmon.the effect of oxytetracycline (otc) treatment on intestinal bacterial populations in juvenile atlantic salmon salmo salar was evaluated. oxytetracycline was administered by way of medicated feed to fish held in experimental tanks. restriction fragment length polymorphism and sequencing of 16s rdna from isolates were used to analyze the intestinal microbiota before, during, and after otc administration. the microbiota from untreated fish was more diverse, consisting mainly of pseudomonas, acineto ...200818942594
in vitro and in vivo studies of the use of some medicinal herbals against the pathogen aeromonas hydrophila in goldfish.aeromonas hydrophila is a ubiquitous and opportunistic bacterial pathogen that produces ulcerative dermatitis under stress conditions and inflicts severe losses on global fisheries and fish culture. this study evaluates the antimicrobial potency of aqueous and ethanolic decoction (individual extract) and concoction (mixed extract) of three common medicinal herbs, turmeric curcuma longa, tulsi plant ocimum sanctum, and neem azadirachta indica, against the in vitro growth of a. hydrophila. among t ...200818942593
multiplex pcr detection of enterotoxin genes in aeromonas spp. from suspect food samples in northern taiwan.aeromonads possess an array of virulence factors and are causative agents of a number of human infections. among them, genes of one cytotoxic (act) and two cytotonic (alt, ast) enterotoxins are implicated in a human diarrheal disease. a rapid, specific, simultaneous detection of these enterotoxin genes in suspected food poisoning samples is not yet reported. hence, a multiplex pcr assay was designed to amplify the cytotoxic (act), heat-labile cytotonic (alt), and heat-stable cytotonic (ast) ente ...200818939759
isolation of mannose-binding c-type lectin from sea lamprey (petromyzon marinus) plasma and binding to aeromonas salmonicida.the sea lamprey (petromyzon marinus) is a parasitic cartilaginous fish of the north american great lakes and a predator of many bony fish species of commercial importance to the fishing industry. mannose-binding c-type lectin (mbl) was isolated by mannan-agarose affinity chromatography from sea lamprey plasma. mannose-binding lectin has not before been identified and quantitated in the plasma of this sea lamprey species. the affinity-purified and 2-me reduced lamprey mbl showed two bands of 35kd ...200818937980
effect of a probiotic bacterium bacillus circulans pb7 in the formulated diets: on growth, nutritional quality and immunity of catla catla (ham.).bacillus circulans pb7, isolated from the intestine of catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of catla catla. the effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. catla fingerlings (ave. wt. 6.48 +/- 0.43 g) were fed diets supplemented with 2 x 10(4) (feed c1), 2 x 10(5) (feed c2), and 2 x 10(6) (feed c3) b. circulans pb 7 cells per 100 g feed for 60 days at 5% of the body weight ...200918931930
molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from aeromonas media.a new tyrosinase was isolated from aeromonas media strain ws and purified to homogeneity. the purified tyrosinase, termed tyra, had a molecular mass of 58 kda and an isoelectric point of 4.90. it exhibited optimal monophenol and diphenol oxidase activities under basic conditions (ph>8.0). tyra had a relatively higher affinity to diphenol substrate l-dihydroxyphenylalanine (l-dopa) than many other tyrosinases. edta or glutathione notably inhibited the enzymatic activities of tyra, whereas triton ...200918931836
mutation in the s-ribosylhomocysteinase (luxs) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of aeromonas hydrophila.a diarrheal isolate ssu of aeromonas hydrophila produces a cytotoxic enterotoxin (act) with cytotoxic, enterotoxic, and hemolytic activities. our laboratory has characterized from the above aeromonas strain, in addition to act, the type 3- and t6-secretion systems and their effectors, as well as the genes shown to modulate the production of ai-1-like autoinducers, n-acylhomoserine lactones (ahls) involved in quorum sensing (qs). in this study, we demonstrated the presence of an s-ribosylhomocyst ...200818930130
virulence and resistance markers in clinical and environmental aeromonas strains isolated in romania.the virulence and resistance (r) features of 37 aeromonas strains from diarrheal cases and 150 from the aquatic environment (isolated during cold and warm season) were tested at different incubation temperatures (4 degrees c, 28 degrees c and 37 degrees c). when incubated at 4 degrees c temperature, the aeromonasstrains isolated during the cold season expressed the highest number of virulence factors by comparison with the strains isolated during warm season and from diarrhoeal cases, the virule ...200718928066
influence of carp intestinal mucus molecular size and glycosylation on bacterial adhesion.the first step of the pathogenesis of many infectious diseases is the colonisation of the mucosal surface by the pathogen. bacterial colonisation of the mucosal surface is promoted by adherence to high molecular weight mucus glycoproteins. we examined the effect of carp intestinal mucus glycoproteins on the adhesion of different bacteria. the bacteria used were 3 strains of aeromonas hydrophila, and a. salmonicida, edwardsiella tarda and yersinia ruckeri. all bacteria adhered to mucus, but at va ...200818924378
[new selective indicator medium for detection of aeromonas and plesiomonas: unisc agar].we evaluated a new selective indicator medium (unisc agar) for isolation of classical enteropathogens, aeromonas spp and plesiomonas shigelloides. the xylose fermentation capacity is indicated by a yellow color (fermenting agents) or blue (no fermenting agent). this, together with the oxidase test, establishes it as an indicator for detecting aeromonas and plesiomonas shigelloides. its productivity and selectivity, as assessed using the absolute count index and relative count index, indicate it ...200818853024
normal intestinal flora of wild nile crocodiles (crocodylus niloticus) in the okavango delta, botswana.bacterial and fungal cultures were performed from cloacal swabs collected from 29 wild nile crocodiles, captured in the okavango delta, botswana. sixteen species of bacteria and 6 fungal species were cultured. individual crocodiles yielded 1-4 bacterial species, and 0-2 fungal species. the most commonly isolated bacteria were microbacterium, enterococcus faecalis, aeromonas hydrophila, and escherichia coli. no salmonellae were cultured. the most commonly occurring fungus was cladosporium. severa ...200818846850
[the distribution and antimicrobial resistance tendency of pathogens associated with diarrhea in beijing].to monitor the constituents and resistant tendency of bacterial pathogens isolated from diarrheal patients in our hospital form 1994 to 2005 to offer the basis for guiding epidemiologic study, vaccination research and clinical treatment.200818843955
analysis of 16s rrna gene mutations in a subset of aeromonas strains and their impact in species delineation.characterization of 999 aeromonas strains using a published 16s rdna rflp identification method showed that 8.1% of the strains produced unexpected (hereafter called "atypical") restriction patterns, making their identification uncertain. atypical patterns were due to the presence of nucleotide polymorphisms among the rrn operons of the 16s rrna gene (so-called microheterogeneities). double sequencing signals at certain positions revealed the nucleotide composition was responsible for the microh ...200818843597
bacterial enteropathogens of neonates admitted to an urban diarrhoeal hospital in bangladesh.data on the aetiology of diarrhoea in neonates are scarce, especially from developing countries including bangladesh. a retrospective review of the electronic database of the microbiology laboratory of the international centre for diarrhoeal disease research, bangladesh (icddr,b), was carried out to examine enteropathogens associated with diarrhoea in neonates. stool specimens of the neonates on admission to the dhaka hospital of icddr,b were collected and sent to the laboratory for direct plati ...200918840632
phylogenetic evidence suggests that strains of aeromonas hydrophila subsp. dhakensis belong to the species aeromonas aquariorum sp. nov.three strains of aeromonas hydrophila subspecies dhakensis, including the type strain, were subjected to phylogenetic analysis by sequencing gyrb, rpod, and 16s rrna genes and compared with all known aeromonas species. the obtained gyrb and rpod phylogenetic trees clearly suggested that these a. hydrophila subsp. dhakensis strains indeed belong to the species a. aquariorum. this finding may indicate that, at the time of "dhakensis" subspecies description, the strains were incorrectly identified ...200918839248
[detecting pathogenic aeromonas hydrophila in fish by triplex pcr].to develop a rapid pcr method to detect pathogenic aeromonas hydrophila in fish.200818837375
molecular and functional characterization of a novel cc chemokine in large yellow croaker (pseudosciaena crocea).a full-length cc chemokine cdna was isolated from large yellow croaker (pseudosciaena crocea) by expressed sequence tag (est) analysis (lyccc2). its open reading frame (orf) is 282 nucleotides (nt), encoding a polypeptide of 93 amino acids (aa). the deduced lyccc2 contains a 20-aa signal peptide and a 73-aa mature polypeptide, which possesses the typical arrangement of four cysteines as found in other known cc chemokines (c(34), c(35), c(61) and c(75)). it shares 17.4-40.4% and 10.0-24.5% aa seq ...200818835451
physico-chemical and microbial characteristics of the coral reef environment of the gulf of mannar marine biosphere reserve, india.investigation on physico-chemical parameters and bacteial characteristics of the coral reef environs of the gulf of mannar biosphere reserve was studied. the study found the influence of different physico-chemical parameters on one another and also on the distribution of the total heterotrophic bacteria (thb) in the coral reef areas. nutrients exhibited considerable seasonal and spatial variations with influence on the bacterial population. coral reef areas recorded higher bacterial population d ...200818831378
diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of coriandrum sativum.the total heterotrophic bacteria, actinomycetes and fungus were enumerated from the rhizosphere and non-rhizosphere soil of 50 selected locally available medicinal plants in and around bharathiar university. in all the plants, population of microorganism were higher in the rhizosphere soil than in the non rhizosphere soil. among the microorganisms, bacterial population was higher in number followed by fungus and actinomycetes. of the medicinal plants, the maximum rhizosphere effect was observed ...200818831345
ompa is an adhesion factor of aeromonas veronii, an optimistic pathogen that habituates in carp intestinal tract.in the present study, we focused on one of the aeromonas veronii isolates that exhibited marked adhesion onto carp intestine and studied its membrane-associated proteins for their possible involvement in mucosal adhesion.200818828791
chinese herbs (astragalus radix and ganoderma lucidum) enhance immune response of carp, cyprinus carpio, and protection against aeromonas hydrophila.the effect of chinese herbs (astragalus radix and ganoderma lucidum) on immune response of carp was investigated. fish were fed diets containing astragalus (0.5%), ganoderma (0.5%) and combination of two herbs (astragalus 0.5% and ganoderma 0.5%) for 5 weeks. other groups of fish were vaccinated (i.p.) against aeromonas hydrophila/aeromonas salmonicida (shering plough, essex, u.k.) at the beginning of the experiment and fed the same diets as described above. control fish (negative control) and f ...200918817878
expressed sequence tags (ests) from immune tissues of turbot (scophthalmus maximus) challenged with pathogens.the turbot (scophthalmus maximus; scophthalmidae; pleuronectiformes) is a flatfish species of great relevance for marine aquaculture in europe. in contrast to other cultured flatfish, very few genomic resources are available in this species. aeromonas salmonicida and philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in ...200818817567
concurrent infection with ranavirus, batrachochytrium dendrobatidis, and aeromonas in a captive anuran colony.four species (dendrobates auratus, phyllobates terribilis, pyxicephalus adspersus, and rhacophorus dennysi) of captive anurans with a clinical history of lethargy and inappetence were found dead and were submitted for necropsy. gross lesions included irregular patches of sloughed skin and rare dermal ulcerations. histologic findings included epidermal proliferation that was most pronounced on the digits and that included intracytoplasmic chytrid organisms. bacteria were often associated with the ...200818817009
enzymatic characterization of vibrio alginolyticus strains isolated from bivalves harvested at venice lagoon (italy) and guanabara bay (brazil).the aquatic ecosystem is the natural habitat of microorganisms including vibrio and aeromonas genus which are pathogenic to human and animals. in the present investigation the frequency of these bacteria and the enzymatic characteristics of 34 vibrio alginolyticus strains isolated from bivalves harvested in venice lagoon (italy) and guanabara bay (brazil) were carried out from november 2003 to february 2004. the mussels' samples were submitted to enrichment in alkaline peptone water (apw) added ...200818813756
[ants in a hospital environment and its importance as vector of bacteria].the external ant community of hospital municipal de morrinhos, in morrinhos, goiás state, was characterized by the low rates of richness, diversity, dominance and equity of species abundance. pheidole sp.1, a polygynic species was numerically dominant in this environment, although it coexists with potentially competitive species. this ant species prevailed within all hospital departments and its space-time distribution was a little aggregated (variance/mean ratio = 1.102, chi2 = 29.38, p < 0.01) ...200818813751
incidence of bacterial enteropathogens among hospitalized diarrhea patients from orissa, india.bacteriological analysis of 1,551 stool/rectal swabs from all age groups of diarrhea patients of different hospitals of orissa from january 2004 to december 2006 was carried out using standard procedures. among all enteropathogens isolated in 886 culture-positive samples, escherichia coli constituted 75.5%, including 13.2% pathogenic e. coli; vibrio cholerae o1 constituted 17.3%; v. cholerae o139, 1%; shigella spp., 4.5% (shigella flexneri type 6, 2.9%, s. dysenteriae type i, 0.7%, s. sonnei, 0. ...200818806340
molecular diversity of class 1 integrons in human isolates of aeromonas spp. from southern taiwan.this work studies antimicrobial resistance and class 1 integrons of aeromonas spp. in human isolates from southern taiwan. pcr amplification and dna sequence analyses were performed to characterize the gene cassette regions of the class 1 integron in 204 isolates of aeromonas hydrophila, 36 isolates of a. sobria, 23 isolates of a. veronii, and 4 isolates of a. caviae. by using southern hybridization with an inti1 probe to determine the presence of class 1 integrons in the 9 isolates of aeromonas ...200818806339
production of monoclonal antibodies specific to major outer membrane protein of edwardsiella tarda.edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and gastro and extra-intestinal infections in humans. monoclonal antibodies (mabs) were produced against outer membrane proteins (omps) of e. tarda et-7, isolated from diseased snakehead (ophiocephalus punctatus). two stable hybridoma clones, designated as 3f10 and 2c3 mabs were found to be potentially specific for e. tarda by indirect enzyme linked immunosorbent assay (elisa). these mabs recognized major immunogenic omp ...201018804863
antibacterial agents and heavy metal resistance in gram-negative bacteria isolated from seawater, shrimp and sediment in iskenderun bay, turkey.the aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from seawater, sediment and shrimps, and to determine if there is a relationship between antibiotic and heavy metal resistance. we undertook studies in 2007 in the industrially polluted iskenderun bay, on the south coast of turkey. the resistance of 236 gram-negative bacterial isolates (49 from seawater, 90 from sediment and 97 from shrimp) to ...200818804847
the genome of aeromonas salmonicida subsp. salmonicida a449: insights into the evolution of a fish pathogen.aeromonas salmonicida subsp. salmonicida is a gram-negative bacterium that is the causative agent of furunculosis, a bacterial septicaemia of salmonid fish. while other species of aeromonas are opportunistic pathogens or are found in commensal or symbiotic relationships with animal hosts, a. salmonicida subsp. salmonicida causes disease in healthy fish. the genome sequence of a. salmonicida was determined to provide a better understanding of the virulence factors used by this pathogen to infect ...200818801193
case reports: fatal necrotizing fasciitis caused by aeromonas sobria in two diabetic patients.we report two rare cases of aeromonas sobria necrotizing fasciitis with sepsis in patients with diabetes. in both cases, immediate fasciotomy was performed and appropriate empiric antimicrobial therapy and intensive care were administered. however, the two patients died on day 2 and day 11, respectively, after admission as a result of multiple organ failure. when patients present with a rapid onset of skin necrosis and progressive sepsis, an aeromonas sobria infection or vibrio infection should ...200918800212
identification of the antimicrobial substances produced by solanum palinacanthum (solanaceae).to find out natural antimicrobial agents as alternative in therapeutics and to preserve food, the methanol extract of solanum palinacanthum aerial parts was submitted to purification steps guided by antibacterial and antifungal assays. as a consequence, the flavonoid rutin and 3,5-dicaffeoylquinic acid were isolated by column chromatography and high performance liquid chromatography, and identified by mass and nuclear magnetic resonance spectrometry. minimal inhibitory concentrations (mic) of th ...200818797795
in vitro evaluation on adherence and antimicrobial properties of a candidate probiotic clostridium butyricum cb2 for farmed fish.to characterize the antimicrobial and adhesion ability of candidate probiotic clostridium butyricum cb2 for farmed fish in vitro.200818795975
immune responses and enhanced disease resistance in chinese drum, miichthys miiuy (basilewsky), after oral administration of live or dead cells of clostridium butyrium cb2.clostridium butyrium cb2 isolated from chickens was tested as a potential fish probiotic in the chinese drum, miichthys miiuy. fish were fed live (cb), dead cb2 (d-cb) cells (10(8) cells g(-1)) or spent culture supernatant (scs), for 30 days and challenged with vibrio anguillarum or aeromonas hydrophila. survival was higher in both the cb and the cb-d fed groups, but the scs group was not significantly different from the control. after feeding live or dead cb2 cells, there was increase in phagoc ...200818786030
surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006.the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ...200818786028
microbial levan in the diet of labeo rohita hamilton juveniles: effect on non-specific immunity and histopathological changes after challenge with aeromonas hydrophila.a 60-day feeding trial was conducted to study the immuno-protective effect of microbial levan on labeo rohita juveniles challenged with aeromonas hydrophila. six purified diets were prepared with different levels of microbial levan: control (no levan), t1 (basal + 0.25%), t2 (basal + 0.50%), t3 (basal + 0.75%), t4 (basal + 1%) and t5 (basal + 1.25%), fed to six groups of fish in triplicate. among the treatment groups the haemoglobin content and total leucocyte count were increased with a dietary ...200818786027
polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida.the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ...200818783128
identification of iron regulated genes in the fish pathogen aeromonas salmonicida subsp. salmonicida: genetic diversity and evidence of conserved iron uptake systems.in this study we applied the fur titration assay (furta) to isolate iron regulated genes in aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish. we have identified genes for siderophore biosynthesis and for ferri-siderophore transport, some of which have been previously described in this species. a gene for a hitherto uncharacterized functional hemin receptor hute was identified, but its inactivation did not affect significantly the use of hemin as sole iron sou ...200918774242
plasmid-mediated quinolone resistance in aeromonas allosaccharophila recovered from a swiss lake.to search for plasmid-mediated qnr genes among waterborne environmental aeromonas spp. recovered from switzerland.200818772162
effect of bacterial interference on biofilm development by legionella pneumophila.in the ecology of legionella pneumophila a crucial role may be played by its relationship with the natural flora; thus we investigated the interactions between legionella and other aquatic bacteria, particularly within biofilms. among 80 aquatic bacteria screened for the production of bacteriocin-like substances (blss), 66.2% of them were active against l. pneumophila. the possible effect of some of these aquatic bacteria on the development and stability of l. pneumophila biofilms was studied. p ...200818769851
bacterial communities on electron-beam pt-deposited electrodes in a mediator-less microbial fuel cell.the content of a bacterial consortium found on an electron beam (e-beam) pt-deposited electrode in a mediator-less microbial fuel cell (mfc) using glucose and glutamate as fuel is reported in this paper. the e-beam pt-deposited electrode and electrochemically active bacteria (eab) consortium were developed to improve the mediator-less mfc performance. denaturing gradient gel electrophoresis (dgge), restriction fragment length polymorphism (rflp), and 16s rrna sequencing were used to identify the ...200818767694
determination of viability of aeromonas hydrophila in increasing concentrations of sodium chloride at different temperatures by flow cytometry and plate count technique.aeromonads in waters and foods can represent a risk to human health. factors such as sodium chloride concentration and temperature can affect growth and viability of several food and water-borne pathogens. the behaviour of an aeromonas hydrophila strain in the presence of 1.7%, 3.4% and 6% nacl concentrations at 24 degrees c and 4 degrees c was studied over a 188 day period. viability and membrane potential were assessed by flow cytometry; growth was evaluated by plate count technique. flow cyto ...200818765166
cloning, nucleotide sequence and phylogenetic analyses, and tissue-specific expression of the transferrin gene in cirrhinus mrigala infected with aeromonas hydrophila.transferrin partial complementary dnas were cloned from the livers of five species in four genera of indian carps (indian major carp species: labeo rohita, catla catla and cirrhinus mrigala; medium carp: puntius sarana; minor carp: labeo bata) subsequent to polymerase chain reaction amplification with published heterologous primers or self-designed primers derived from conserved regions of transferrin cdna sequences. the partial transferrin cdnas of the five species of carps had sizes from 624 t ...200918718662
production of c5a by asp, a serine protease released from aeromonas sobria.aeromonas sobria causes pus and edema at sites of infection. however, the mechanisms underlying these effects have not been elucidated. c5a, the amino-terminal fragment of the complement 5th component (c5), mimics these events. to investigate the involvement of c5a in the pathophysiology of a. sobria infection, we examined release of c5a from human c5 by a serine protease (asp), a putative virulence factor secreted by this bacterium. c5 incubated with enzymatically active asp induced neutrophil ...200818714034
the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase from haemophilus influenzae contains two active-site histidine residues.the catalytic and structural properties of the h67a and h349a dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase (dape) from haemophilus influenzae were investigated. on the basis of sequence alignment with the carboxypeptidase from pseudomonas sp. strain rs-16, both h67 and h349 were predicted to be zn(ii) ligands. the h67a dape enzyme exhibited a decreased catalytic efficiency (180-fold) compared with wild-type (wt) dape towards n-succinyldiaminopimelic acid. no catalytic activity w ...200918712420
quantification of hurdles: predicting the combination of effects -- interaction vs. non-interaction.combination of disparate as well as related antimicrobial effects constitutes the concept of hurdle technology. quantification of combined effects, including claims of synergy, can be accomplished using surface response modelling, as is frequently done and reported. the gamma hypothesis, however, states that the relative effects of different antimicrobial factors combine independently. studies performed using time to detection have shown that the gamma hypothesis is an adequate foundation for th ...200818703251
contamination of community water sources by potentially pathogenic vibrios following sea water inundation.potentially pathogenic members of the vibrionaceae family including vibrio cholerae and vibrio parahemolyticus were isolated from domestic sources of drinking water in coastal villages following sea water inundation during the tsunami in southern india. phenotypic and genotypic studies were done to confirm the identity and detection of toxins. vibrio-gyr (gyrase b gene) was detected in all sixteen vibrio isolates. toxin regulating genes i.e.: ctx gene, tdh gene, and trh gene, however were not de ...200718697592
[the importance of aeromonads as a human pathogen].the genus aeromonas belongs to the family aeromonadaceae of the order aeromonadales and consists of 14 phenospecies and 17 genomospecies. they are gram-negative, oxidase-positive, facultatively anaerobic and glucose-fermenting rods. members of the mesophilic genus aeromonas spp. are ubiquitous in the aquatic environment and can be isolated from fresh, brackish and marine water. in nutrient-enriched waters they can attain large populations, particularly in the warm seasons at higher temperatures. ...200818696148
characterization of the digestive-tract microbiota of hirudo orientalis, a european medicinal leech.fda-approved, postoperative use of leeches can lead to bacterial infections. in this study, we used culture-dependent and culture-independent approaches to characterize the digestive-tract microbiota of hirudo orientalis. surprisingly, two aeromonas species, a. veronii and a. jandaei, were cultured. uncultured rikenella-like bacteria were most similar to isolates from hirudo verbana.200818689513
necrotizing fasciitis and sepsis caused by aeromonas hydrophila after crush injury of the lower extremity.aeromonas hydrophila is a motile gram-negative bacillus found in water sources that typically causes minor skin infections or gastroenteritis in humans. there are sporadic reports of cases of sepsis or necrotizing fasciitis caused by a. hydrophila but no other cases of severe infection secondary to trauma. the mortality rate of septic shock caused by a. hydrophila approaches 100%.200818687029
pseudomonas and aeromonas isolates from domestic reptiles and study of their antimicrobial in vitro sensitivity. 200818683065
occurrence of aeromonas hydrophila in wild birds.aeromonas hydrophila was isolated from 135 of a total of 1226 wild birds. it was the only bacterial species in eight birds, while other bacteria, mainly enterobacteriaceae, staphylococci and/or streptococci were identified in all other birds. the rate of isolation from aquatic birds (18.5%) was higher (p<0.001) than from birds of terrestrial habitats (3.4%). infection may also depend on dietary habits: 7.0% of the granivorous and herbivorous species, 8.4% of the omnivores and 12.0% of carnivorou ...198918679900
the dihydrolipoamide dehydrogenase of aeromonas caviae st exhibits nadh-dependent tellurite reductase activity.potassium tellurite (k(2)teo(3)) is extremely toxic for most forms of life and only a limited number of organisms are naturally resistant to the toxic effects of this compound. crude extracts prepared from the environmental isolate aeromonas caviae st catalize the in vitro reduction of teo32- in a nadh-dependent reaction. upon fractionation by ionic exchange column chromatography three major polypeptides identified as the e1, e2, and e3 components of the pyruvate dehydrogenase (pdh) complex were ...200818675788
contribution of food deprivation to the immune response in rainbow trout (oncorhynchus mykiss) vaccinated against cryptobia salmositica and aeromonas salmonicida.the aims of the present study were to determine (a) the effectiveness of an attenuated live cryptobia salmositica vaccine; (b) the effects of food deprivation on the immune response and its duration in rainbow trout (oncorhynchus mykiss) immunised with a live c. salmositica vaccine or with a killed aeromonas salmonicida vaccine. the fish were divided into three groups (i, ii and iii; 14 fish per group), those in groups i and ii were under food deprivation (0.40% of body weight), while group iii ...200818669247
use of vitamin c as an immunostimulant. effect on growth, nutritional quality, and immune response of labeo rohita (ham.).teleost fish lack the enzyme for endogenous synthesis of ascorbic acid (aa), an essential micronutrient for fish. the aim of this study was to examine the effect of higher levels of dietary vitamin c on growth, nutritional quality, and immunomodulation in the indian major carp, rohu (labeo rohita). four groups of l. rohita were fed experimental diets containing either no vitamin c (control) or supplemented with vitamin c at 500 mg kg(-1) (exp-1), 1000 mg kg(-1) (exp-2), or 1500 mg kg(-1) (exp-3) ...200818665463
sdia, an n-acylhomoserine lactone receptor, becomes active during the transit of salmonella enterica through the gastrointestinal tract of turtles.luxr-type transcription factors are typically used by bacteria to determine the population density of their own species by detecting n-acylhomoserine lactones (ahls). however, while escherichia and salmonella encode a luxr-type ahl receptor, sdia, they cannot synthesize ahls. in vitro, it is known that sdia can detect ahls produced by other bacterial species.200818665275
structure of asce and induced burial regions in asce and ascg upon formation of the chaperone needle-subunit complex of type iii secretion system in aeromonas hydrophila.in the type iii secretion system (t3ss) of aeromonas hydrophila, the putative needle complex subunit ascf requires both putative chaperones asce and ascg for formation of a ternary complex to avoid premature assembly. here we report the crystal structure of asce at 2.7 a resolution and the mapping of buried regions of asce, ascg, and ascf in the asceg and ascefg complexes using limited protease digestion. the dimeric asce is comprised of two helix-turn-helix monomers packed in an antiparallel fa ...200818662905
necrotizing fasciitis in patients with liver cirrhosis: predominance of monomicrobial gram-negative bacillary infections.necrotizing fasciitis (nf), a rare but severe infection, usually occurs in individuals with underlying chronic illness, but its clinical presentation among cirrhotic patients is infrequently discussed. forty-two cirrhotic patients with 47 episodes of nf between 1995 and 2006 were analyzed. their mean age was 55.6 years, with male preponderance (34 patients, 81%). lower extremities were mainly involved (70%). of 42 episodes with identified pathogens, 41 (97%) were monomicrobial infections and wer ...200818653302
[application of gyrb in the identification of closely related bacteria-- a review].the single-copied gyrb gene, encoding the subunit b of gyrase and distributing universally in all bacteria, has the average substitution rate of 0.7%-0.8% per million years. it's proved that this region could be used to discriminate and identify the closely related species of different bacteria such as pseudomonoas, bacillus, vibrio, enterobacteriaceae, mycobacteria, aeromonas, lactic acid bacteria et.al. it also can be used of quantitative or restriction fragment analysis of bacteria with the a ...200818652307
monitoring bacterial and archaeal community shifts in a mesophilic anaerobic batch reactor treating a high-strength organic wastewater.shifts in bacterial and archaeal communities, associated with changes in chemical profiles, were investigated in an anaerobic batch reactor treating dairy-processing wastewater prepared with whey permeate powder. the dynamics of bacterial and archaeal populations were monitored by quantitative real-time pcr and showed good agreement with the process data. a rapid increase in bacterial populations and a high rate of substrate fermentation were observed during the initial period. growth and regrow ...200818647356
bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards.a survey was carried out to investigate the aerobic bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards maintained at the national avian research center, abu dhabi, united arab emirates. none of the houbara or rufous-crested bustards sampled were bred in captivity by the center but four of the kori bustards sampled were captive-bred. faecal, cloacal, oropharyngeal and skin samples (from under the wing) from clinically ...199618645872
expanded-spectrum beta-lactamase per-1 in an environmental aeromonas media isolate from switzerland. 200818644966
influence of poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) on growth and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.as a new member of polyhydroxyalkanoate (pha) family, the novel polyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate) (p(3hb-4hb-3hhx)) was produced by recombinant aeromonas hydrophila 4ak4 and used for the first time to test its biocompatibility. it was shown that p(3hb-4hb-3hhx) had higher hydrophobicity, surface energy, and rougher surface than the well-studied polymers poly(l-lactic acid) (pla) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx). human mesenchym ...200918642327
immune response of zebrafish (danio rerio) against a newly isolated bacterial pathogen aeromonas hydrophila.a strain of aeromonas hydrophila associated with unusual mortalities in zebrafish (danio rerio) culture facilities was isolated, identified and characterized. in challenge experiments, adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection with viable bacteria and its extracellular products (ecps) reaching very high mortalities in a few hours. the infection, by the viable bacteria or the ecps, caused cell death in kidney, due to the cytotoxic and haemolytic activities ...200818640853
abundance of sewage-pollution indicator and human pathogenic bacteria in a tropical estuarine complex.studies on abundance and types of various pollution indicator bacterial populations from tropical estuaries are rare. this study was aimed to estimate current levels of pollution indicator as well as many groups of human pathogenic bacteria and their seasonal variations in different locations in mandovi and zuari rivers in the central west coast of india. the sampling covered the estuarine and upstream regions of these rivers representing premonsoon (may 2005), monsoon (september 2006) and post- ...200918633722
therapeutic potential of two probiotics in inflammatory bowel disease as observed in the trinitrobenzene sulfonic acid model of colitis.the pathogenesis of inflammatory bowel disease is thought to be a multifactorial process. one of the leading hypotheses is that an imbalance in normal gut flora induces an excessive immune response and contributes to inflammation in the gastrointestinal tract. administration of probiotic bacteria reduces symptoms in patients suffering from inflammatory bowel diseases, probably via both manipulation of the microflora and stimulation of the intestinal immune system. in the current study the therap ...200818633675
major histocompatibility complex based resistance to a common bacterial pathogen of amphibians.given their well-developed systems of innate and adaptive immunity, global population declines of amphibians are particularly perplexing. to investigate the role of the major histocompatibility complex (mhc) in conferring pathogen resistance, we challenged xenopus laevis tadpoles bearing different combinations of four mhc haplotypes (f, g, j, and r) with the bacterial pathogen aeromonas hydrophila in two experiments. in the first, we exposed ff, fg, gg, gj, and jj tadpoles, obtained from breedin ...200818629002
emerging extra-intestinal infections with aeromonas hydrophila in coastal region of southern karnataka.aeromonas species are gram-negative rods usually isolated from the gastrointestinal tract. they have been occasionally reported as a cause of extra-intestinal infections such as cellulitis, cholangitis, necrotizing fascitis, meningitis, bacteremia, or peritonitis in both immunocompetent and immunocompromised patients.200818626167
thrombotic microangiopathy as a complication of medicinal leech therapy.medicinal leeches (hirudo medicinalis) are increasingly used as a useful therapeutic option in various medical and surgical settings. the potential complications associated with this therapy include infections with aeromonas species, bleeding, anemia, and allergic reactions. we describe a patient who developed thrombotic microangiopathy and acute renal failure following leech therapy. it is proposed that the series of nonhuman proteins within leech saliva may be capable of injuring the endotheli ...200818622333
real-time pcr detection of pathogenic microorganisms in roof-harvested rainwater in southeast queensland, australia.in this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of escherichia coli, enterococci, clostridium perfringens, and bacteroides spp. in rainwater obtained from tanks in southeast queensland, australia. samples were also tested using real-time pcr (with sybr green i dye) for the presence of potential pathogenic microorganisms. of the 27 rainwater samples tested, 17 (63%), 21 (78%), 13 (48%), and 24 (89%) were positive for e. coli, en ...200818621865
aeromonas tecta sp. nov., isolated from clinical and environmental sources.five aeromonas strains, isolated from both clinical and environmental sources and characterized by a polyphasic approach, including phylogenetic analysis derived from gyrb, rpod, and 16s rrna gene sequencing, as well as dna-dna hybridization, extensive biochemical and antibiotic susceptibility tests, were recognized as members of an unknown, or undescribed, aeromonas species. these "aeromonas eucrenophila-like" strains were closely related to the species a. eucrenophila and aeromonas encheleia, ...200818617346
lung colonization with aeromonas hydrophila in cystic fibrosis believed to have come from a tropical fish tank. 200818607019
microbial challenge promotes the regenerative process of the injured central nervous system of the medicinal leech by inducing the synthesis of antimicrobial peptides in neurons and microglia.following trauma, the cns of the medicinal leech, unlike the mammalian cns, has a strong capacity to regenerate neurites and synaptic connections that restore normal function. in this study, we show that this regenerative process is enhanced by a controlled bacterial infection, suggesting that induction of regeneration of normal cns function may depend critically upon the coinitiation of an immune response. we explore the interaction between the activation of a neuroimmune response and the proce ...200818606660
isolation of putative probionts from cod rearing environment.survival problems are encountered at early stages of intensive fish rearing and antibiotics are widely used to remedy the situation. probiotics may provide a potential alternative method to protect larvae from opportunistic and pathogenic bacteria and promote a balanced environment. this study was designed to search for new probiotics to target this critical period in cod rearing. potential probionts were selected from the natural microbiota of cod aquacultural environment. the selection was bas ...200818599226
a micro-dilution method for detecting oxytetracycline-resistant bacteria in marine sediments from salmon and mussel aquaculture sites and an urbanized harbour in atlantic canada.a micro-dilution technique with changes in optical density (od) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 microg otc (oxytetracycline)ml(-1) was applied to determine growth inhibition (gi) in mixed bacterial strains cultured from samples of marine sediments and salmon feed pellets. growth of control cultures (aeromonas salmonicida) was inhibited at all otc concentrations. some feed pellet samples and under-cage sediments from salmon aquac ...200818589456
solubilization of fish proteins using immobilized microbial cells.cells of bacillus megaterium, aeromonas hydrophila, and pseudomonas marinoglutinosa were immobilized in calcium alginate. the immobilized cells secreted protease when held in fish meat suspension in water. the enzyme synthesis by the entrapped cells was supported by small amounts of soluble nutrients present in the meat. the secreted protease solubilized the fish meat, solubilization being optimum at ph range of 7.5 to 9.5 and at 50 degrees c. under these conditions immobilized b. megaterium was ...198918588026
insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology.wastewater treatment plants (wwtps) are a reservoir for bacteria harbouring antibiotic resistance plasmids. to get a comprehensive overview on the plasmid metagenome of wwtp bacteria showing reduced susceptibility to certain antimicrobial drugs an ultrafast sequencing approach applying the 454-technology was carried out. one run on the gs 20 system yielded 346,427 reads with an average read length of 104 bases resulting in a total of 36,071,493 bases sequence data. the obtained plasmid metagenom ...200818586057
combined physico-chemical treatments based on enterocin as-48 for inactivation of gram-negative bacteria in soybean sprouts.enterocin as-48 was tested for decontamination of soybean sprouts against gram-negative bacteria. although treatment with bacteriocin alone had no effect on salmonella enterica, a synergistic antimicrobial effect was detected at ph 9.0 and in combination with moderate heat treatment. greatest inactivation was achieved for sprouts heated for 5 min at 65 degrees c in an alkaline (ph 9.0) enterocin as-48 solution of 25 microg/ml. bactericidal activity against s. enterica increased greatly when ente ...200818577412
an explanation for the effect of inoculum size on mic and the growth/no growth interface.the inoculum effect (ie) is the phenomenon observed where changes in the inoculum size used in an experiment alters the outcome with respect to, for example, the minimum inhibitory concentration of an antimicrobial or the growth/no growth boundary for a given set of environmental conditions. various hypotheses exist as to the cause of the ie such as population heterogeneity and quorum sensing, as well as the null hypothesis - that it is artefactual. time to detection experiments (ttd) were carri ...200818573556
toxin-screening and identification of bacteria isolated from highly toxic marine gastropod nassarius semiplicatus.bacteria isolated from a highly toxic sample of gastropod nassarius semiplicatus in lianyungang, jiangsu province in july 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. the toxicity of the gastropod sample was 2 x 10(2)mouse unit (mu) per gram of tissue (wet weight). high concentration of tetrodotoxin (ttx) and its analogues (ttxs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography c ...200818573274
identification of differentially expressed immune-relevant genes in chinese soft-shelled turtle (trionyx sinensis) infected with aeromonas hydrophila.expressed sequence tag (est) analysis is an efficient tool for gene discovery and profiling gene expression. aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. in order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in chinese soft-shelled turtle (trionyx sinensis) experimentally infected with a. hydrophila by suppressio ...200818572252
chemical investigation of finlaysonia obovata: part i--a rare triterpene acid showing antibacterial activity against fish pathogens.the antibacterial screening of extracts of the leaves of finlaysonia obovata with hexane, chloroform and alcohol was carried out against fresh water fish pathogenic bacteria viz., micrococcus sp. (multidrug resistant strain), aeromonas hydrophila, pseudomonas aeruginosa, vibrio alginolyticus, staphylococcus aureus, escherichia coli, edwardsiella tarda by disc-assay method. the hexane and chloroform extracts were found active against four and five pathogens, respectively. the highly active chloro ...200818569719
structural basis for the broad-spectrum inhibition of metallo-beta-lactamases by thiols.the development of broad-spectrum metallo-beta-lactamase (mbl) inhibitors is challenging due to structural diversity and differences in metal utilisation by these enzymes. analysis of structural data, followed by non-denturing mass spectrometric analyses, identified thiols proposed to inhibit representative mbls from all three sub-classes: b1, b2 and b3. solution analyses led to the identification of broad spectrum inhibitors, including potent inhibitors of the cpha mbl (aeromonas hydrophila). s ...200818563261
[microbiological studies of spiced beef burgers in tripoli city, libyan arab jamahiriya].we investigated the microbiological quality of uncooked and cooked spiced beef burger in restaurants and fast food places in tripoli city and surrounding areas. thus 151 samples (92 cooked and 59 uncooked) were analysed microbiologically. the results showed that all samples were highly contaminated with bacteria; 74.5%, 28.8%, 27.1% and 18.6% of the uncooked samples were contaminated with escherichia coli, aeromonas spp., staphylococcus aureus and e. coli o157:h7 respectively. additionally 11.9% ...200818557465
removal of nitrogen, phosphorus, and organic pollutants from water using seeding type immobilized microorganisms.to study the possibility of removing nitrogen, phosphorus, and organic pollutants using seeding type immobilized microorganisms.200818548855
[characterization of aeromonas spp isolates from newborns hospitalized].aeromonas spp is recognized as pathogenic to humans after consumption of contaminated water and food. in the present investigation, 2,323 rectal swab samples from newborns hospitalized in rio de janeiro were evaluated with a view to isolating aeromonas. the samples were collected and sent to the national reference laboratory for cholera and other bacterial intestinal infections, at the oswaldo cruz institute of the oswaldo cruz foundation. the swabs were subjected to enrichment in alkaline pepto ...200818545840
antimicrobial peptides derived from hemoglobin are expressed in epithelium of channel catfish (ictalurus punctatus, rafinesque).the beta-chain of the respiratory protein hemoglobin (hbbeta), has recently been identified in novel sites, including mammalian macrophages and alveolar epithelium, as well as in gill microsomes of fish. however, the functional significance of extra-erythrocytically expressed hemoglobin has been unclear. here we show inducible expression and upregulation of antimicrobial peptides (amps) homologous to hbbeta in the gill epithelium of channel catfish (ictalurus punctatus) in response to parasitic ...200818538841
serum crp-like protein profile in common carp cyprinus carpio challenged with aeromonas hydrophila and escherichia coli lipopolysaccharide.the potential of c-reactive protein (crp)-like proteins to be used as a biomarker of health status in cultured carp obtained from various european fish lines has been assessed. varying crp-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. crp-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). serum levels of crp-like protein in carp were observ ...200818538390
identification of heme uptake genes in the fish pathogen aeromonas salmonicida subsp. salmonicida.aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish, can use heme as the sole iron source. we applied the fur titration assay to isolate a cluster including six genes hutazxbcd that showed similarity to heme uptake genes of other gram-negative bacteria, and three genes orf123 of unknown function. the spatial organization of these nine genes, arranged in five transcriptional units, was similar to that of a homologous cluster in a. hydrophila. when a tonb system w ...200818535817
conformational changes in the metallo-beta-lactamase imis during the catalytic reaction: an epr spectrokinetic study of co(ii)-spin label interactions.metallo-beta-lactamases are responsible for conferring antibiotic resistance on certain pathogenic bacteria. in consequence, the search for inhibitors that may be useful in combating antibiotic resistance has fueled much study of the active sites of these enzymes. there exists circumstantial evidence that the binding of substrates and inhibitors to metallo-beta-lactamases may involve binding to the organic part of the molecule, in addition to or prior to binding to one or more active site metal ...200818528987
c1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis.except for the complement c1q, the immunological functions of other c1q family members have remained unclear. here we describe zebrafish c1q-like, whose transcription and translation display a uniform distribution in early embryos, and are restricted to mid-hind brain and eye in later embryos. in vitro studies showed that c1q-like could inhibit the apoptosis induced by actd and chx in epc cells, through repressing caspase 3/9 activities. moreover, its physiological roles were studied by morpholi ...200818514183
plasmid-mediated qnrs2 determinant from a clinical aeromonas veronii isolate. 200818505860
development of multiplex pcr assay for simultaneous detection of five bacterial fish pathogens.a multiplex polymerase chain reaction (pcr) method was designed for the simultaneous detection of the five major fish pathogens, aeromonas hydrophila, aeromonas salmonicida subsp. salmonicida, flavobacterium columnare, renibacterium salmoninarum, and yersinia ruckeri. each of the five pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. the detection limits of the multiplex pcr was in the range of 2, 1, 1, 3, and 1cfu for a. hydrophila, a. salmo ...200818499358
mutational analysis of the zinc- and substrate-binding sites in the cpha metallo-beta-lactamase from aeromonas hydrophila.the subclass b2 cpha (carbapenemase hydrolysing aeromonas) beta-lactamase from aeromonas hydrophila is a zn(2+)-containing enzyme that specifically hydrolyses carbapenems. in an effort to evaluate residues potentially involved in metal binding and/or catalysis (his(118), asp(120), his(196) and his(263)) and in substrate specificity (val(67), thr(157), lys(224) and lys(226)), site-directed mutants of cpha were generated and characterized. our results confirm that the first zinc ion is in interact ...200818498253
comparative assessment upon dye removal capability of indigenous bacterial strains from lanyang plain in northeast taiwan.this study provides a first attempt from a geological and ecological perspective to look forward isolations of indigenous strains with the decolorization capability from the most biodiverse region in taiwan for dye-laden wastewater treatment. serial selections were conducted by a specific use of the fungicide nystatin and model azo dye c.i. reactive red 141 (rr141) during isolation. several bacterial strains with the excellent capability of azo dye decolorization were predominantly isolated from ...200918495337
enterobacteriaceae and related organisms isolated from shell eggs collected during commercial processing.in the united states, commercial shell eggs are washed and graded before retail. since passage of the egg products inspection act in 1971, processing guidelines have been set to ensure that external and internal characteristics are maintained. however, less is known about how commercial processing affects the safety of shell eggs. to identify enteric bacteria entering plants and persisting throughout processing, eggs were collected from 3 us commercial shell egg-processing plants on 3 separate v ...200818493013
protein expression by aeromonas hydrophila during growth in vitro and in vivo.expression of aeromonas hydrophila cellular and extracellular products (ecps) was examined following culture of the bacterium in vitro, in tryptic soy broth (tsb), and in vivo, in dialysis tubing placed within the peritoneal cavity of common carp (cyprinus carpio l.). whole cell (wc), outer membrane proteins (omps) and ecp components of the bacteria were analysed by 1 dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (1d sds-page). additionally, 2d sds-page was used to analy ...200818490132
family association between immune parameters and resistance to aeromonas hydrophila infection in the indian major carp, labeo rohita.seven innate immune parameters were investigated in 64 full-sib families (the offspring of 64 sires and 45 dams) from two year-classes of farmed rohu carp (labeo rohita). survival rates were also available from aeromonas hydrophila infection (aeromoniasis) recorded in controlled challenge tests on a different sample of individuals from the same families. due to strong confounding between the animal additive genetic effect and the family effects (common environmental+non-additive genetic), reliab ...200818486488
clinical significance of spontaneous aeromonas bacterial peritonitis in cirrhotic patients: a matched case-control study.although aeromonas species are known to cause bacteremia in patients with cirrhosis, less is known about spontaneous bacterial peritonitis (sbp) caused by aeromonas species in these patients.200818484880
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