Publications

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[on the diagnosis of gram-negative aerobic bacilli]. 196314114135
6-aminopenicillanic acid: inhibition of destruction of cephalosporin c by bacteria.6-aminopenicillanic acid, from which all the true penicillins are derived, inhibits the destruction of cephalosporin c by certain microorganisms. this inhibition is probably specific for "cephalosporinase." the enzymatic deacetylation of cephalosporin c is not affected by the acid.196414107444
development of antibodies against aeromonas salmonicida in trout. 196314106298
[study of the lipolytic activity of the metabolic fluids of a strain of aeromonas hydrophila and determination of tributyrinase]. 196314089090
the genus aeromonas: methods for identification. 196314072386
studies on the enteropathogenic, facultatively halophilic bacteria, vibrio parahaemolyticus. i. morphological, cultural and biochemical properties and its taxonomical position. 196314071901
[on the significance of aeromonades and so-called paracolobactra for water bacteriology]. 196314069497
mixed polar and peritrichous flagellation of marine bacteria. 196314051812
quantitative approach to the study of bacterial species.liston, j. (university of washington, seattle), w. wiebe, and r. r. colwell. quantitative approach to the study of bacterial species. j. bacteriol. 85:1061-1070. 1963.-the stability of strains of various species of gram-negative, rod-shaped bacteria was investigated by subculturing each strain through ten different media, testing the substrains so produced through a standard series of tests, and analyzing the resulting data by electronic computer. strain stability was shown to be high [similarit ...196314043996
[on the biochemical properties of anaerogenic aeromonas]. 196214039064
[on the biochemical properties of aeromonas salmonicida]. 196114039063
acetylmethylcarbinol production and the classification of aeromonads associated with ulcerative diseases of ectothermic vertebrates.page, l. a. (biological research institute, san diego, and university of california, davis). acetylmethylcarbinol production and the classification of aeromonads associated with ulcerative diseases of ectothermic vertebrates. j. bacteriol. 84:772-777. 1962.-quantitative colorimetric tests were made for acetylmethylcarbinol (amc) production by 14 aeromonas isolates from ulcerous lesions of snakes, lizards, frogs, and other animals, and by 27 cultures of "identified" aeromonads. the tests revealed ...196213941061
[serological studies in experimental infection by microorganisms of the genus aeromonas]. 196113915281
the effect of x-irradiation on goldfish. i. the effect of x-irradiation on survival and susceptibility of the goldfish, carassius auratus, to infection by aeromonas salmonicida and gyrodactylus spp. 196213911544
[isolation of aeromonas from 2 cases of acute diarrhea]. 196213895794
taxonomy of the fish furunculosis organism. 196213888294
[the production of putrefaction of the caudal fin in fish by the action of aeromonas punctata]. 196113880926
[the causes of an outbreak of putrefaction of the caudal fin in fish and its treatment with kanamycin]. 196113880925
[the action in vitro of kanamycin on aeromonas punctata]. 196113880924
[study in vitro of the action of kanamycin on bacteria pathogenic for fish]. 196113880923
[the genus aeromonas of kluyver and van niel (1936). (collective review on its significance and on methods of detection)]. 196113880760
taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species.colwell, r. r. (university of washington, seattle) and j. liston. taxonomic analysis with the electronic computer of some xanthomonas and pseudomonas species. j. bacteriol. 82:913-919. 1961.-forty organisms, including 16 xanthomonas spp., 5 strains of pseudomonas solanacearum, 4 strains of p. aeruginosa, 6 strains of p. fluorescens, 5 freshly isolated strains of pseudomonads, as well as the american type culture collection strains of flavobacterium aquatile, vibrio cuneatus, vibrio metschnikovii ...196113880721
[aeromonas strains as pathogenic organisms, their sensitivity to antibiotics and sulfonamides]. 196213877076
[studies on differentiation of microorganisms of the genera aeromonas and vibrio mueller (antibiotics, sulfonamides, vibriostatic 0/129)]. 196113877075
the identification and separation of aeromonas liquefaciens from pseudomonas fluorescens and related organisms occurring in diseased fish.taxonomy of the motile species of the genus aeromonas is briefly discussed. it is suggested that aeromonas organisms, isolated from outbreaks of red mouth of trout, red sore of pike, infectious abdominal dropsy and hemorrhagic septicemia of warm water fish, and which show acid and gas in glucose broth, production of 2,3-butanediol hydrogen sulfide from motility sulfide medium, presence of cytochrome oxidase, and hydrolysis of starch, be designated as aeromonas liquefaciens. these tests also serv ...196113874617
a note on the genus aeromonas. 195913856383
[further studies on the genus aeromonas]. 196013849330
[immunological response of rabbits to experimental infection with bacteria of the aeromonas type]. 196013767506
pseudomonas--an attempt at a general classification. 196113764624
observations on the specificities of extracellular antigens of the genera aeromonas and serratia. 196113762795
taxonomic relationships among the pseudomonads.colwell, r. r. (university of washington, seattle), and j. liston. taxonomic relationships among the pseudomonads. j. bacteriol. 82:1-14. 1961.-an electronic computer technique, utilizing the adansonian principle that every feature should have equal weight, was applied in an effort to derive a taxonomy of the pseudomonas-achromobacter group of gram-negative, asporogenous, rodlike bacteria. the validity of the general method was tested by an analysis of 40 well defined strains, principally derive ...196113694873
aeromonas hydrophila (pseudomonas hydrophila) nrc 491 and nrc 492 established as aerobacter cloacae. 196013694038
[antihemolysin studies and their differential diagnostic significance for the aeromonas group]. 196013691259
[serological studies with aeromonas strains]. 196113691258
[biological studies on microorganism of the genus aeromonas]. 195813625982
studies of aeromonas formicans crawford comb. nov. from soluble oil emulsions. 195713416178
taxonomic study of sucrose-positive aeromonas jandaei-like isolates from faeces, water and eels: emendation of a. jandaei carnahan et al. 1992.fourteen sucrose-positive aeromonas jandaei-like isolates from fresh water and reared european eels were subjected to a polyphasic study to determine their taxonomic position. numerical taxonomy was used to analyse phenotypic data obtained for these isolates and 43 type and reference strains representative of recognized aeromonas species. the a. jandaei cluster (phenon 1) was defined at 81.6 % similarity (s(j)); this included the a. jandaei-like isolates, the sucrose-positive strain aeromonas ve ...200313130026
[comparative in vitro activity of ertapenem against aerobic and anaerobic bacteria].the in vitro activity of ertapenem (mk-0826), a new carbapenem, was studied against 389 aerobic microorganisms (187 enterobacteriaceae, 15 aeromonas spp., 42 staphylococcus spp., 43 streptococcus spp., 15 enterococcus spp., 30 haemophilus spp., 15 moraxella catarrhalis, 12 neisseria gonorrhoeae, 15 pseudomonas aeruginosa and 15 acinetobacter spp.) and 54 anaerobic isolates (15 clostridium spp., 12 peptostreptococcus spp. and 27 fragilis group bacteroides recovered from four spanish hospitals. er ...200312973459
the aeromonas salmonicida a-layer protein is an important protective antigen in oil-adjuvanted vaccines. 200312969658
distribution and retention of antigens of aeromonas salmonicida in atlantic salmon (salmo salar l.) vaccinated with a deltaaroa mutant or formalin-inactivated bacteria in oil-adjuvant.in this study we report the differences in distribution and retention of aeromonas salmonicida antigens after vaccination with two different vaccines. parr of atlantic salmon (salmo salar) were given intraperitoneal injections of either a commercial, monovalent furunculosis vaccine (apoject) or live, attenuated a. salmonicida (deltaaroa). fish were sampled at weeks 2, 4 and 12 post-vaccination and head kidney and spleen were collected. presence of lps and 16s rdna in isolated leukocytes were inv ...200312969656
misidentification of aeromonas veronii biovar sobria as vibrio alginolyticus by the vitek system.to find the cause of misidentification of aeromonads when using the vitek system.200312969502
the effects of temperature, water activity and ph on the growth of aeromonas hydrophila and on its subsequent survival in microcosm water.the influence of temperature, water activity and ph on the growth of aeromonas hydrophila, and on its survival after transfer in nutrient-poor water were assessed.200312969295
cloning, expression and characterization of a beta-agarase gene from a marine bacterium, pseudomonas sp. sk38.a gene (paga) encoding beta-agarase from pseudomonas sp. sk38 was cloned and expressed in escherichia coli. the structural gene consists of 1011 bp encoding 337 amino acids with a predicted molecular weight of 37326 and has a signal peptide of 18 amino acids. the deduced amino acid sequence showed 57% and 58% homology to beta-agarase from pseudoalteromonas atalntica and aeromonas sp., respectively. the recombinant enzyme was purified and biochemically characterized. the enzyme had maximum activi ...200312967006
development and maturation of the immune system in zebrafish, danio rerio: a gene expression profiling, in situ hybridization and immunological study.the development and maturation of the immune system in zebrafish was investigated using immune-related gene expression profiling by quantitative real-time polymerase chain reaction, in situ hybridization (ish), immunoglobulin (ig) detection by immuno-affinity purification and western blotting as well as immersion immunization experiments. ikaros expression was first detected at 1 day post-fertilization (dpf) and thereafter increased gradually to more than two-fold between 28 and 42dpf before dec ...200412962979
substrate specificity, metal binding properties, and spectroscopic characterization of the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase from haemophilus influenzae.the catalytic and structural properties of divalent metal ion cofactor binding sites in the dape-encoded n-succinyl-l,l-diaminopimelic acid desuccinylase (dape) from haemophilus influenzae were investigated. co(ii)-substituted dape enzyme was 25% more active than the zn(ii)-loaded form of the enzyme. interestingly, mn(ii) can activate dape, but only to approximately 20% of the zn(ii)-loaded enzyme. the order of the observed k(cat) values are co(ii) > zn(ii) > cd(ii) > mn(ii) >ni(ii) approximatel ...200312962500
antagonistic activity of aeromonas media strain a199 against saprolegnia sp., an opportunistic pathogen of the eel, anguilla australis richardson.a bacteriocin-like inhibitory substance (blis) produced by aeromonas media strain a199 inhibited the growth of saprolegnia sp. in vitro, an opportunistic pathogen isolated from affected eels, anguilla australis (richardson). the presence of blis in solid media inhibited the growth of the vegetative state of the aquatic mould as well as the germination of cysts. uninhibited growth was, however, observed in the presence of inactive blis, suggesting that the in vitro antagonism derived from the bli ...200312962224
oral administration of formalin-inactivated cells of aeromonas hydrophila a3-51 controls infection by atypical a. salmonicida in goldfish, carassius auratus (l.).there has been increasing interest in the use of probiotics in aquaculture to control fish diseases (e.g. douillet langdon 1994; gildberg, mikkelsen, sandaker & ringo 1997; kennedy, tucker, neidic, vermeer, cooper, jarrell & sennett 1998; deschrijver & ollevier 2000; robertson, o'dowd, burrells, williams & austin 2000), and a diverse range of gram-positive (such as carnobacterium inhibens) (robertson et al. 2000) and gram-negative bacteria, including vibrio alginolyticus (garriques & arevalo 199 ...200312962220
use of dead probiotic cells to control furunculosis in rainbow trout, oncorhynchus mykiss (walbaum). 200312962213
analysis of exotoxins produced by atypical isolates of aeromonas salmonicida, by enzymatic and serological methods.in this study, exotoxins produced by 62 aeromonas salmonicida strains and the bacterium haemophilus piscium were analysed. enzymatic assays, zymograms and serological detection were used to monitor secretion by bacterial strains of the previously described exotoxins p1, gcat and asap1 and also the extracellular p2 metallo-gelatinase and a serine caseinase, which is different from the p1 protease and has not yet been characterized. based on the results, the strains were divided into five groups. ...200312962208
antimicrobial susceptibility testing of aquatic bacteria: quality control disk diffusion ranges for escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 at 22 and 28 degrees c.quality control (qc) ranges for disk diffusion susceptibility testing of aquatic bacterial isolates were proposed as a result of a multilaboratory study conducted according to procedures established by the national committee for clinical laboratory standards (nccls). ranges were proposed for escherichia coli atcc 25922 and aeromonas salmonicida subsp. salmonicida atcc 33658 at 22 and 28 degrees c for nine different antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, gentam ...200312958263
genetic characterization of a novel metallo-beta-lactamase gene, blaimp-13, harboured by a novel tn5051-type transposon disseminating carbapenemase genes in europe: report from the sentry worldwide antimicrobial surveillance programme.in 2001, as part of the sentry worldwide antimicrobial surveillance programme, pseudomonas aeruginosa 86-14571a was isolated at a hospital in rome from a cancer patient with a bloodstream infection. the isolate was resistant to all antibiotics except amikacin, and displayed an imipenem mic of 64 mg/l that decreased to 8 mg/l in the presence of edta. the resistance determinant was investigated.200312951335
three small, cryptic plasmids from aeromonas salmonicida subsp. salmonicida a449.the nucleotide sequences of three small (5.2-5.6 kb) plasmids from aeromonas salmonicida subsp. salmonicida a449 are described. two of the plasmids (pasa1 and pasa3) use a cole2-type replication mechanism while the third (pasa2) is a cole1-type replicon. insertions in the rep protein and oriv region of the cole2-type plasmids provide subtle differences that allow them to be maintained compatibly. all three plasmids carry genes for mobilization (mobabcd), but transfer genes are absent and are pre ...200312932739
tolerance and starvation induced cross-protection against different stresses in aeromonas hydrophila.aeromonas hydrophila is sometimes considered as a controversial human pathogen and reported to be susceptible to food processing procedures and environmental stresses. in this study, we have shown that early stationary phase cells of a. hydrophila were readily killed during up shifts in temperature (in the range 50-70 degrees c), the course of drying (at relative humidity, temperature and brightness of the laboratory) and after 5 min exposure to 20%, 30% and 40% v/v ethanol. however, this bacter ...200312927714
production of poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) with flexible 3-hydroxyhexanoate content in aeromonas hydrophila cgmcc 0911.aeromonas hydrophila cgmcc 0911 isolated from lake water was found to be able to synthesize a polyhydroxyalkanoate (pha) copolymer (phbhhx) consisting of 3-hydroxybutyrate (hb) and 4-6 mol% 3-hydroxyhexanoate (hhx). the wild-type bacterium accumulated 49% phbhhx containing 6 mol% hhx in terms of cell dry weight (cdw) when grown on lauric acid for 48 h. when a. hydrophila cgmcc 0911 expressed the acyl-coa dehydrogenase gene ( yafh) of escherichia coli, the recombinant strain could accumulate 47% ...200412920488
molecular cloning, expression of orange-spotted grouper goose-type lysozyme cdna, and lytic activity of its recombinant protein.lysozyme acts as a non-specific innate-immunity molecule against the invasion of bacteria pathogens. a leukocyte cdna library of orange-spotted grouper epinephelus coioides was constructed and the goose-type (g-type) lysozyme cdna was isolated. the complete cdna consists of an open reading frame of 585 bp encoding a protein of 194 amino acids. this protein shows a 72.2% amino acid sequence identity with the flounder g-type lysozyme. similar to most other species, the glu catalytic residue in g-t ...200312911059
glyceraldehyde-3-phosphate dehydrogenase, a glycolytic enzyme present in the periplasm of aeromonas hydrophila.this is the first report describing the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (gapdh), as a protein associated with the cell envelope of a gram-negative bacterium (aeromonas hydrophila). dose-dependent gapdh activity was detected in whole bacterial cells from exponentially growing cultures, indicating that an active form of gapdh is located outside the plasma membrane. this activity represents roughly 10-20% of total cell activity, and it is not reduced by pretreatment of t ...200312906360
aeromonas infection of the hepatobiliary system: report of 15 cases and review of the literature.aeromonas species cause both intestinal and extraintestinal disease. we reviewed hospital laboratory and medical records to identify patients with aeromonas infection of the hepatobiliary or pancreatic system. analysis of data from our hospital, as well as a review of the published literature, yielded a total of 41 episodes in 39 patients, and the features of these episodes are described. the most common manifestation of aeromonas hepatobiliary infection among all reported cases was cholangitis ...200312905134
type iii secretion genes in aeromonas salmonicida subsp salmonicida are located on a large thermolabile virulence plasmid.type iii secretion genes in aeromonas salmonicida subsp. salmonicida are located on a large plasmid of approximately 140 kb. cultivation of this organism at elevated temperatures such as 25 degrees c can, however, result in loss of this plasmid. this is accompanied by a loss of virulence for cultured fish cells.200312904401
fatal aeromonas hydrophila septicemia in a 16-year-old patient with thalassemia. 200312902930
alteration of chain length substrate specificity of aeromonas caviae r-enantiomer-specific enoyl-coenzyme a hydratase through site-directed mutagenesis.aeromonas caviae r-specific enoyl-coenzyme a (enoyl-coa) hydratase (phaj(ac)) is capable of providing (r)-3-hydroxyacyl-coa with a chain length of four to six carbon atoms from the fatty acid beta-oxidation pathway for polyhydroxyalkanoate (pha) synthesis. in this study, amino acid substitutions were introduced into phaj(ac) by site-directed mutagenesis to investigate the feasibility of altering the specificity for the acyl chain length of the substrate. a crystallographic structure analysis of ...200312902277
curvularia haloperoxidase: antimicrobial activity and potential application as a surface disinfectant.a presumed antimicrobial enzyme system, the curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. in the presence of hydrogen peroxide, curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. the curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (pseudomonas spp., escherichia coli, serratia marcescens, aeromonas salmonicida, s ...200312902249
chloride dependence of growth in bacteria.chloride is an abundant anion on earth but studies analyzing a possible function of chloride in prokaryotes are scarce. to address the question, we have tested 44 different gram-negative and gram-positive bacteria for a chloride dependence or chloride stimulation of growth. none required chloride for growth at their optimal growth (salt) conditions. however, in hyperosmotic media containing high concentrations of na+, 11 bacteria (aeromonas hydrophila, bacillus megaterium, bacillus subtilis, cor ...200312900036
putative virulence factors of atypical aeromonas salmonicida isolated from arctic charr, salvelinus alpinus (l.), and european grayling, thymallus thymallus (l.).atypical aeromonas salmonicida (aas) causes generalized lethal infections in farmed arctic charr, salvelinus alpinus (l.), and european grayling, thymallus thymallus (l.), and is thus a serious threat for culture of these fish species. virulence factors were studied among isolates of aas from arctic charr (n = 20), european grayling (n = 19) and other fish species (n = 20), of which 48 were of finnish and 11 of swedish origin. all isolates produced an a-layer. extracellular products (ecp) of the ...200312899410
protection against atypical furunculosis in atlantic halibut, hippoglossus hippoglossus (l.); comparison of a commercial furunculosis vaccine and an autogenous vaccine.atlantic halibut, hippoglossus hippoglossus (l.), was shown to be sensitive to infection by three different isolates of aeromonas salmonicida ssp. achromogenes in pre-challenge tests using intraperitoneal (i.p.) and intramuscular (i.m.) injections as well as bath challenges. a commercial furunculosis vaccine, alphaject 1200, and an autogenous vaccine, aas, based on the challenge strain, induced immune protection as shown in challenge tests 8 weeks post-immunization. the survival rate of vaccinat ...200312899408
bacteria that express lateral flagella enable dissection of the multifunctional roles of flagella in pathogenesis.flagella are much more than organelles of locomotion and have multiple roles that contribute to pathogenesis. bacteria, such as vibrio parahaemolyticus and aeromonas spp., that possess two distinct flagellar systems (a polar flagellum for swimming in liquid and lateral flagella for swarming over surfaces) are relatively uncommon and provide ideal models for the independent investigation of the contributions of these different types of motility and other flagellar functions to virulence and how t ...200312892877
evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using pva-immobilized cell beads.an immobilized mixed culture (aeromonas hydrophila, comamonas testosteroni, and acinetobacter baumannii) was prepared by entrapment into phosphorylated polyvinyl alcohol (pva) gel beads. the unsteady-state diffusion mechanism in a gel bead was applied to estimate the effective diffusion coefficients (d(e)) and the partition coefficients (k(p)) of azo dye. in addition, a simple method was developed to determine the intrinsic kinetic parameters of immobilized cells from observed reaction rates and ...200312889022
molecular and biochemical characterization of a novel class a beta-lactamase (her-1) from escherichia hermannii.escherichia hermannii showed a low level of resistance to amoxicillin and ticarcillin, reversed by clavulanate, and a moderate susceptibility to piperacillin but was susceptible to all cephalosporins. a bla gene was cloned and encoded a typical class a beta-lactamase (her-1, pi 7.5), which shares 45, 44, 41, and 40% amino acid identity with other beta-lactamases, aer-1 from aeromonas hydrophila, mal-1/cko-1 from citrobacter koseri, and tem-1 and len-1, respectively. no ampr gene was detected. on ...200312878539
tolerance to various toxicants by marine bacteria highly resistant to mercury.bacteria highly resistant to mercury isolated from seawater and sediment samples were tested for growth in the presence of different heavy metals, pesticides, phenol, formaldehyde, formic acid, and trichloroethane to investigate their potential for growth in the presence of a variety of toxic xenobiotics. we hypothesized that bacteria resistant to high concentrations of mercury would have potential capacities to tolerate or possibly degrade a variety of toxic materials and thus would be importan ...200312876655
aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis b.gram-negative bacilli of the genus aeromonas are widespread in aquatic environments and can be responsible for human infections. although aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, aeromonas septicaemia remains an uncommon finding, often associated with serious underlying disease and predominantly related to the species aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae. here, a case of a. veronii biovar ve ...200312867570
molecular characterization and quantitative analysis of superoxide dismutases in virulent and avirulent strains of aeromonas salmonicida subsp. salmonicida.aeromonas salmonicida subsp. salmonicida is a facultatively intracellular gram-negative bacterium that is the etiological agent of furunculosis, a bacterial septicemia of salmonids that causes significant economic loss to the salmon farming industry. the mechanisms by which a. salmonicida evades intracellular killing may be relevant in understanding virulence and the eventual design of appropriate treatment strategies for furunculosis. we have identified two open reading frames (orfs) and relate ...200312867441
phylogenetic affiliation of aeromonas culicicola mtcc 3249(t) based on gyrb gene sequence and pcr-amplicon sequence analysis of cytolytic enterotoxin gene.we determined the gyrb gene sequences of all 17 hybridizations groups of aeromonas. phylogenetic trees showing the evolutionary relatedness of gyrb and 16s rrna genes in the type strains of aeromonas were compared. using this approach, we determined the phylogenetic position of aeromonas culicicola mtcc 3249(t), isolated from midgut of culex quinquefasciatus. in the gyrb based-analysis a. culicicola mtcc 3249(t) grouped with a. veronii whereas, it grouped with a. jandaei in the 16s rrna based tr ...200312866846
intracellular vacuolation induced by culture filtrates of plesiomonas shigelloides isolated from environmental sources.potential virulence factors produced by culture filtrates of plesiomonas shigelloides isolated from water were investigated.200312859758
recent increase in antibiotic-resistant microorganisms in patients with spontaneous bacterial peritonitis adversely affects the clinical outcome in korea.recently, antibiotic-resistant microorganisms have been increasingly noted in korean patients with spontaneous bacterial peritonitis (sbp). the present study investigated the changing pattern of antibiotic resistance and its effects on the clinical outcome in treating sbp.200312859722
lateral flagella are required for increased cell adherence, invasion and biofilm formation by aeromonas spp.two types of flagella are responsible for motility in mesophilic aeromonas strains. a polar unsheathed flagellum is expressed constitutively that allows the bacterium to swim in liquid environments and, in media where the polar flagellum is unable to propel the cell, aeromonas express peritrichous lateral flagella. recently, southern blot analysis using a dna probe based on the aeromonas caviae sch3n lateral flagellin gene sequence showed a good correlation between strains positive for the dna p ...200312855171
a beta-n-acetylglucosaminyl phosphate diester residue is attached to the glycosylphosphatidylinositol anchor of human placental alkaline phosphatase: a target of the channel-forming toxin aerolysin.glycosylphosphatidylinositol (gpi)-anchored proteins are ubiquitous in eukaryotes. the minimum conserved gpi core structure of all gpi-anchored glycans has been determined as etn-po4-6manalpha1-2manalpha1-6manalpha1-4glcn-myo-inositol-po3h. human placental alkaline phosphatase (ap) has been reported to be a gpi-anchored membrane protein. ap carries one n-glycan, (neuacalpha2-->3)2gal2glcnac2man3glcnac(+/-fuc)glcnac, and a gpi anchor, which contains an ethanolamine phosphate diester group, as a s ...200312851398
dietary bovine lactoferrin induces changes in immunity level and disease resistance in asian catfish clarias batrachus.the effects of including bovine lactoferrin (lf) in the diet of the asian catfish (clarias batrachus) on specific and non-specific immunity as well as disease resistance were investigated. the catfish were fed four different diets for 2 weeks: a commercial diet as control and the same diet supplemented with 50, 100 and 200mg bovine lf/kg feed. after 1 and 2 weeks, serum bacterial agglutination titre against aeromonas hydrophila as a measure of specific immunity; natural serum haemolysin titre, l ...200312842607
environmental isolates of aeromonas spp. harboring the caga-like gene of helicobacter pylori.we investigated the presence of caga-like gene of helicobacter pylori in environmental isolates of aeromonas spp. from different water samples of calcutta, india, by colony hybridization using a caga-specific dna probe and by pcr with caga-specific primers. nucleotide sequencing of five pcr products revealed 97 to 98% homology to canonical caga of h. pylori 26695 as well as to four clinical h. pylori strains from calcutta. the caga-like gene of the environmental isolates was unstable in laborato ...200312839817
complement resistance is essential for colonization of the digestive tract of hirudo medicinalis by aeromonas strains.from the crop of the medicinal leech, hirudo medicinalis, only aeromonas veronii bv. sobria can be cultured consistently. serum-sensitive a. veronii mutants were unable to colonize h. medicinalis, indicating the importance of the mammalian complement system for this unusual simplicity. complementation of one selected mutant restored its ability to colonize. serum-sensitive mutants are the first mutant class with a colonization defect for this symbiosis.200312839811
determination of in situ bacterial growth rates in aquifers and aquifer sediments.laboratory and field-scale studies with stained cells were performed to monitor cell growth in groundwater systems. during cell division, the fluorescence intensity of the protein stain 5-(and 6-)-carboxyfluorescein diacetate succinimidyl ester (cfda/se) for each cell is halved, and the intensity can be tracked with a flow cytometer. two strains of bacteria, comamonas sp. strain da001 and acidovorax sp. strain oy-107, both isolated from a shallow aquifer, were utilized in this study. the change ...200312839747
acute bacterial gastroenteritis: a study of adult patients with positive stool cultures treated in the emergency department.to investigate the presenting clinical features of acute bacterial gastroenteritis in adult patients treated as outpatients in the emergency department (ed), and the pathogens responsible in this setting and population; and to identify the frequency with which positive stool culture result changes management.200312835343
hydrodynamic characteristics of immobilized cell beads in a liquid-solid fluidized-bed bioreactor.this study examined the hydrodynamic characteristics of a liquid-solid fluidized-bed bioreactor using elastic particles (pva gel beads) of various diameters as carriers. the drag coefficient-reynolds number, velocity-voidage, and expansion index-reynolds number relationships observed during fluidization of pva gel beads in a fluidized bed in our experiments were compared with the published results. predictions made from previous correlations were examined with our new experimental findings, reve ...200312827700
bacterial degradation of arsenobetaine via dimethylarsinoylacetate.microorganisms from mytilus edulis (marine mussel) degraded arsenobetaine, with the formation of trimethylarsine oxide, dimethylarsinate and methylarsonate. four bacterial isolates from these mixed-cultures were shown by hplc/hydride generation-atomic fluorescence spectroscopy (hplc/hg-afs) analysis to degrade arsenobetaine to dimethylarsinate in pure culture; there was no evidence of trimethylarsine oxide formation. two of the isolates ( paenibacillussp. strain 13943 and pseudomonas sp. strain ...200312827219
identification of aeromonas hydrophila cytotoxic enterotoxin-induced genes in macrophages using microarrays.a cytotoxic enterotoxin (act) of aeromonas hydrophila possesses several biological activities, and it induces an inflammatory response in the host. in this study, we used microarrays to gain a global and molecular view of the cellular transcriptional responses to act and to identify important genes up-regulated by this toxin. total rna was isolated at 0, 2, and 12 h from act-treated macrophages and applied to affymetrix mgu74 arrays, and the data were processed using a multi-analysis approach to ...200312824169
in vitro and in vivo activities of fluoroquinolones against aeromonas hydrophila.aeromonas hydrophila, an uncommon human pathogen, can cause invasive infections in immunocompromised individuals. as the fluoroquinolones have been shown to be active in vitro against mesophilic aeromonads and clinical experience with the use of fluoroquinolones to treat aeromonads infections is limited, the antimicrobial activities of five selected drugs (ciprofloxacin, gatifloxacin, levofloxacin, lomefloxacin, and moxifloxacin) against a. hydrophila were studied in vitro and in mice. the mics ...200312821471
the effect of intraperitoneally administered recombinant il-1beta on immune parameters and resistance to aeromonas salmonicida in the rainbow trout (oncorhynchus mykiss).the present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (ril-1beta) in vivo. the predicted rainbow trout mature il-1beta peptide was produced as a recombinant protein in escherichia coli. optimal migration of peritoneal leucocytes and ril-1beta induced phagocytosis occurred following intraperitoneal injection of 1 microg of the recombinant protein. moreover, systemic il-1beta, cox-2 and lysozyme ii gene expression was enha ...200312818637
mhc polymorphism and disease resistance in atlantic salmon (salmo salar); facing pathogens with single expressed major histocompatibility class i and class ii loci.few studies have yet addressed the functional aspects of mhc molecules in fish. to lay the foundation for this, we evaluated the association between disease resistance and mhc class i and class ii polymorphism in atlantic salmon. standardized disease challenge trials were performed on a semi-wild atlantic salmon population with subsequent mhc typing and statistical analysis. the pathogens employed were infectious salmon anaemia virus (isav) causing infectious salmon anaemia and the aeromonas sal ...200312811427
aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in thailand.a group of seven sucrose-negative aeromonas strains (referred to as group au) isolated from the internal organs of septicaemic farmed frogs (rana rugulosa) in thailand was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (faflp) and eric-pcr fingerprinting, 16s rdna sequencing, microplate dna-dna hybridizations and extensive phenotypic characterization. comparison of faflp and eric-pcr fingerprints indicated that the group au isolates belonge ...200312807217
phylogenetic analysis of members of the genus aeromonas based on gyrb gene sequences.the phylogenetic relationships of all known species of the genus aeromonas were investigated by using the sequence of gyrb, a gene that encodes the b-subunit of dna gyrase. nucleotide sequences of gyrb were determined from 53 aeromonas strains, including some new isolates, which were also characterized by analysis of the 16s rdna variable regions. the results support the recognition of the family aeromonadaceae, as distinct from plesiomonas shigelloides and other enteric bacteria. this phylogene ...200312807216
occurrence and antibiotic resistance of mesophilic aeromonas in three riverine freshwaters of marrakech, morocco.in order to evaluate the impact of pollution and sewage on the occurrence and antibiotic resistance of mesophilic aeromonads in riverine freshwaters of marrakech, samples were collected from three rivers (oukaimeden, ourika, and tensift) upstream and downstream from the principal bordering villages. during a 2-year study, indicators of pollution increased dramatically in the downstream waters. bacterial indicators (faecal coliforms and faecal streptococci) correlated with mesophilic aeromonads o ...200112805714
cellulose beads bound to cellulose binding domain-fused recombinant proteins; an adjuvant system for parenteral vaccination of fish.a recombinant form of the outer membrane protein (a-layer protein) associated with atypical aeromonas salmonicida was expressed, fused to a cellulose binding domain (cbd) isolated from clostridium cellulovorans. the resultant chimerical protein was bound to either sigmacell 20((r)) or orbicell cellulose particles. common goldfish were injected intraperitoneally with the cellulose-protein complex and blood serum antibody levels produced against a-protein were examined weekly by means of elisa. th ...200312804848
a novel electrochemically active and fe(iii)-reducing bacterium phylogenetically related to aeromonas hydrophila, isolated from a microbial fuel cell.a facultative anaerobic bacterium was isolated from a mediator-less microbial fuel cell fed with artificial wastewater containing acetate and designated as pa3. the isolate was identified as a strain of aeromonas hydrophila based on its biochemical, physiological and morphological characteristics as well as 16s rdna sequence analysis and dna-dna hybridization. pa3 used glucose, glycerol, pyruvate and hydrogen to reduce fe(iii), nitrate and sulfate. cyclic voltammetry showed that pa3 was electroc ...200312799011
a toll-like receptor (tlr) gene that is up-regulated in activated goldfish macrophages.an expressed sequence tag screen of a macrophage activation factor and lipopolysaccharide (lps) stimulated goldfish macrophage subtractive library generated several transcripts of a putative teleost homologue of the toll-like receptor (tlr) family. the full-length tlr cdna was sequenced and is predicted to encode a type i transmembrane protein with an extracellular domain containing leucine rich repeats and a cytoplasmic tail encoding a toll/interleukin-1 receptor domain. these findings indicate ...200312798365
synthesis and biological evaluation of hydroxamate-based inhibitors of glutamate carboxypeptidase ii.a series of hydroxamic acids has been prepared as potential inhibitors of glutamate carboxypeptidase ii (gcp ii). compounds based on a p1' residue (primed-side inhibitors) were more potent than those based on a p1 group (unprimed-side inhibitors). inhibitory potency of the primed-side gcp ii inhibitors was found to be dependent on the number of methylene units between the hydroxamate group and pentanedioic acid. succinyl hydroxamic acid derivative, 2-(hydroxycarbamoylmethyl)pentanedioic acid, is ...200312798312
group 1 beta-lactamases of aeromonas caviae and their resistance to beta-lactam antibiotics.the contribution of beta-lactamase production to beta-lactam antibiotic resistance was examined in an aeromonas caviae mutant strain, selected in vitro by cefotaxime and derived from a wild-type strain isolated in our laboratory from crude sewage. both strains produced beta-lactamase. the mutant strain (ac7m) produced beta-lactamase constitutively, in contrast to the parental strain (ac7), which was inducible by cefoxitin. ac7m was regarded as a mutant from ac7, which over-expressed beta-lactama ...200312795407
[the inhibitory effect of extracts from galla chinensis on marine fouling bacteria].to observe the activity of extracts from galla chinensis with different solvents against marine fouling bacteria in vitro.200312795221
the genus aeromonas: biochemical characteristics, atypical reactions, and phenotypic identification schemes.a total of 193 strains representing 14 different aeromonas genomospecies were evaluated for 63 phenotypic properties to create useful tables for the reference identification of mesophilic aeromonads. only 9 of 62 biochemical tests (14%) yielded uniform results, and the fermentation of certain carbohydrates was found to be linked to specific species. a number of unusual or aberrant properties for the genus aeromonas were also detected in the collection of 428 strains (193 in the phenotypic study, ...200312791848
characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico.a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ...200312781953
comparison of three molecular methods for typing aeromonas popoffii isolates.three typing methods, restriction fragment length polymorphism (rflp) of the 16s-23s intergenic spacer region (isr), pcr amplification of the enterobacterial repetitive intergenic consensus (eric) and of the repetitive extragenic palindromic units (rep), were evaluated for typing 26 isolates of aeromonas popoffii from different geographical origins. when the methods were independently studied, eric showed the highest discriminatory power. when the methods were combined, the best combination of t ...200312777070
simultaneous effects of environmental factors on motile aeromonas dynamics in an urban effluent and in the natural seawater.seasonal dynamics of motile aeromonas in a treated urban effluent and in natural seawater along the sfax coast (mediterranean sea, tunisia) were measured over a year concurrently with seven environmental factors, and compared with those of faecal coliforms. counts for aeromonas from a standard plate count method, ranged from 1.48 x 10(5)cfu.100 ml(-1) to 2.2 x 10(8)cfu.100 ml(-1) in the effluent and from 7.9 x 10(3)cfu.100 ml(-1) to undetectable level in the surface marine waters. contrary to fa ...200312767289
sequence analysis and biochemical characterisation of chromosomal cav-1 (aeromonas caviae), the parental cephalosporinase of plasmid-mediated ampc 'fox' cluster.aeromonas caviae cip 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. this strain produced a cephalosporinase (pi 7.2) and an oxacillinase (pi 8.5). the cephalosporinase gene cav-1 was cloned and sequenced. unlike a. caviae donor, escherichia coli pnce50 transformant producing cav-1 beta-lactamase was resistant to cefoxitin. the deduced protein sequence cav-1 contained 382 amino acids, and shared >96% ...200312757951
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