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structural studies on the r-type lipopolysaccharide of aeromonas hydrophila.a rough strain of aeromonas hydrophila, ah-901, has an r-type lipopolysaccharide with the complete core. the following core structure was established by chemical degradations followed by sugar and methylation analyses along with esims and nmr spectroscopy: [formula: see text] where d-alpha-d-hep and l-alpha-d-hep stand for d-glycero- and l-glycero-alpha-d-manno-heptose, respectively; kdo stands for 3-deoxy-d-manno-oct-2-ulosonic acid; all monosaccharides are in the pyranose form; the degree of s ...200414980820
a type iii secretion system is required for aeromonas hydrophila ah-1 pathogenesis.aeromonas hydrophila is a gram-negative opportunistic pathogen in fish and humans. many bacterial pathogens of animals and plants have been shown to inject anti-host virulence determinants into the hosts via a type iii secretion system (ttss). degenerate primers based on lcrd family genes that are present in every known ttss allowed us to locate the ttss gene cluster in a. hydrophila ah-1. a series of genome walking steps helped in the identification of 25 open reading frames that encode protein ...200414977925
hemolytic and proteolytic activities of aeromonas hydrophila and aeromonas veronii biovar sobria in broth and salmon extract at different temperatures.expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species aeromonas hydrophila and aeromonas veronii biovar sobria. although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log cfu/ml in aerated tryptone soya broth plus yeast extract (tsbye) and salmon extract within the range 4 to 28 degrees c. for both strains in tsbye, v ...200414968959
production of transgenic medaka with increased resistance to bacterial pathogens.cecropins, first identified in silk moth (hyalophora cecropia), are a group of antimicrobial peptides with bactericidal activity against a broad spectrum of bacteria. in this study we investigated whether (1) this group of antimicrobial peptides could exhibit bactericidal activity toward known fish bacterial pathogens and (2) expression of cecropin transgenes in transgenic medaka (oryzias latipas) could result in increasing resistance of the transgenic fish to infection by fish bacterial pathoge ...200214961264
genetic diversity and population structure of aeromonas hydrophila, aer. bestiarum, aer. salmonicida and aer. popoffii by multilocus enzyme electrophoresis (mlee).genetic diversity, genetic relationship, identification and population structure of 120 aeromonas strains (including aer. hydrophila, aer. bestiarum, aer. salmonicida and aer. popoffii) isolated from various sources were studied by analysis of 15 genetic loci by multilocus enzyme electrophoresis (mlee). all 15 loci were polymorphic, with an average of 9.4 alleles per locus and a mean genetic diversity (h) of 0.64. cluster analysis defined at h < or = 0.7 differentiated most of the taxa analysed ...200414871204
the identity of proteus hydrophilus bergey et al. and proteus melanovogenes miles & halnan, and their relation to the genus aeromonas kluyver & van niel. 195114832418
in vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus.the selection of probiotics for aquaculture is usually based on their antagonism towards pathogens. however, other criteria such as growth, attachment to intestinal mucus and production of beneficial compounds should also be considered. we suggest a protocol for the isolation and selection of potential probiotic bacteria based on their in vitro growth characteristics and propose a ranking index (ri) to screen potential aquaculture probionts. we suggest that the lag period and doubling time are t ...200414769479
microbial transformation of pyrethroid insecticides in aqueous and sediment phases.recent studies showed that synthetic pyrethroids (sps) can move via surface runoff into aquatic systems. fifty-six of sp-degrading bacteria strains were isolated from contaminated sediments, of which six were evaluated for their ability to transform bifenthrin and permethrin in the aqueous phase and bifenthrin in the sediment phase. in the aqueous phase, bifenthrin was rapidly degraded by strains of stenotrophomonas acidaminiphila, and the half-life (t1/2) was reduced from >700 h to 30 to 131 h. ...200414768859
isolation and characterization of microorganisms capable of decolorizing various triphenylmethane dyes.various soil and sludge samples collected from the vicinity of textile dyeing industries and waste disposal sites were used for enrichment of microbial population in the presence of triphenylmethane (tpm) dye acid violet-17 (av-17). twenty-five (25) isolates were screened for their ability to decolorize av-17 dye added at a rate of 10 mgl(-1) in mineral salts medium (msm) agar plates. five bacterial isolates belonging to bacillus sp., alcaligenes sp. and aeromonas sp. were selected on the basis ...200414768029
coexpression of genetically engineered 3-ketoacyl-acp synthase iii (fabh) and polyhydroxyalkanoate synthase (phac) genes leads to short-chain-length-medium-chain-length polyhydroxyalkanoate copolymer production from glucose in escherichia coli jm109.polyhydroxyalkanoates (phas) can be divided into three main types based on the sizes of the monomers incorporated into the polymer. short-chain-length (scl) phas consist of monomer units of c3 to c5, medium-chain-length (mcl) phas consist of monomer units of c6 to c14, and scl-mcl phas consist of monomers ranging in size from c4 to c14. although previous studies using recombinant escherichia coli have shown that either scl or mcl pha polymers could be produced from glucose, this study presents t ...200414766582
aeromonas meningitis complicating medicinal leech therapy.medicinal leeches have an important and expanding role in medicine, but infection can complicate their use. we describe a unique case of aeromonas meningitis associated with the use of leech therapy to salvage a skin flap after central nervous system surgery.200414765362
[virulence factors of clinical aeromonas caviae isolates].aeromonas caviae, an ubiquitous aquatic organism, has long been considered to be of low pathogenicity, and its virulence mechanisms are still not clearly understood. twenty-eight a. caviae isolates of clinical origin, most often monomicrobic, were identified in our university hospital over a four year period. patients, mostly immunocompromised, were: eight diarrhoeal infants, 13 diarrhoeal adults, seven bacteraemic adults. adults were frequently suffering from underlying intestinal malignancy, h ...200414761709
the role of bacterial and non-bacterial toxins in the induction of changes in membrane transport: implications for diarrhea.bacterial toxins induce changes in membrane transport which underlie the loss of electrolyte homeostasis associated with diarrhea. bacterial- and their secreted toxin-types which have been linked with diarrhea include: (a) vibrio cholerae (cholera toxin, e1 tor hemolysin and accessory cholera enterotoxin); (b) escherichia coli (heat stable enterotoxin, heat-labile enterotoxin and colicins); (c) shigella dysenteriae (shiga-toxin); (d) clostridium perfringens (c. perfringens enterotoxin, alpha-tox ...200314757199
control of aeromonas on minimally processed vegetables by decontamination with lactic acid, chlorinated water, or thyme essential oil solution.aeromonas is an opportunistic pathogen, which, although in low numbers, may be present on minimally processed vegetables. although the intrinsic and extrinsic factors of minimally processed prepacked vegetable mixes are not inhibitory to the growth of aeromonas species, multiplication to high numbers during processing and storage of naturally contaminated grated carrots, mixed lettuce, and chopped bell peppers was not observed. aeromonas was shown to be resistant towards chlorination of water, b ...200414751681
crystallization and preliminary x-ray analysis of the controller protein c.ahdi from aeromonas hydrophilia.single crystals of purified homodimeric controller protein from aeromonas hydrophilia (c.ahdi) have been grown under several different conditions using vapour diffusion. x-ray diffraction data have been collected using synchrotron radiation from crystals of both the native and a selenomethionine (semet) derivative of the protein. the native crystal form belongs to space group p2(1) and data were collected to a resolution of 2.2 a. two crystal forms of the semet protein have been obtained and wer ...200414747712
enteropathogenic activity and invasion of hep-2 cells by aeromonas caviae clinical isolates.twenty aeromons caviae isolates from stool of children with diarrhea symptoms were examined for virulence-associated properties: production of cytotoxic and cytotonic toxins, and invasive ability. most of a. caviae strains were cytotoxic to vero and cho cells and produced cytotonic toxins which caused elongation of cho cells. moreover, five of a. caviae strains revealed invasive ability towards hep-2 cells.200314743980
molecular characterization of a glucose-inhibited division gene, gida, that regulates cytotoxic enterotoxin of aeromonas hydrophila.by using a mini-transposon, we obtained two mutated strains of a diarrheal isolate, ssu, of aeromonas hydrophila that exhibited a 50 to 53% reduction in the hemolytic activity and 83 to 87% less cytotoxic activity associated with the cytotoxic enterotoxin (act). act is a potent virulence factor of a. hydrophila and has been shown to contribute significantly to the development of both diarrhea and septicemia in animal models. subsequent cloning and dna sequence analysis revealed that transposon i ...200414742556
association of type iii secretion genes with virulence of aeromonas salmonicida subsp. salmonicida.aeromonas salmonicida subsp. salmonicida possesses a number of potential virulence factors, including a recently identified plasmid-encoded type iii secretion system. a number of field isolates of a. salmonicida subsp. salmonicida were examined for the presence of type iii secretion genes. using in vitro experiments, it was found that field isolates containing such genes are cytotoxic to fish cell lines, whereas those that lack these genes are not. using a rainbow trout in vivo model, the virule ...200314735937
role of the 'cre/blr-tag' dna sequence in regulation of gene expression by the aeromonas hydrophila beta-lactamase regulator, blra.to further understand the mechanisms used to regulate expression of the blr regulon of aeromonas hydrophila t429125, including three unlinked beta-lactamase genes, amph, ceph and imih, and to examine the role of the 'cre/blr-tag' dna sequence (ttcac) in transcriptional control exerted by the two-component system, blrab.200414729737
[the clinical features and prognosis of aeromonas septicaemia in hepatic cirrhosis: a report of 50 cases].aeromonas septicaemia complicating cirrhosis is not a common infectious disease. to enhance the knowledge in this aspect, we analysed the clinical features, pathogenetic factors, resistance, treatment and prognosis of aeromonas septicaemia in 50 cases of hepatic cirrhosis.200314728873
suitability of the traditional microbial indicators and their enumerating methods in the assessment of fecal pollution of subtropical freshwater environments.twenty-four freshwater sampling sites (11 river water, 6 spring water, and 7 groundwater) were selected from 4 sampling areas located in the northern and central parts of taiwan. a total of 125 water samples were collected during a 5-month sampling period, and the numbers of total heterotrophic bacteria, total coliforms, fecal coliforms, enterococci, aeromonas hydrophila, and salmonella spp. were enumerated. besides the traditional membrane filtration method, total coliforms and escherichia coli ...200314723262
[spontaneous bacterial peritonitis and bacteraemia due to aeromonas hydrophila].bacterial peritonitis is a frequent complication in cirrhotic patients. amongst the aetiological germs, aeromonas is very infrequent. we present the clinical case of a cirrhotic patient with peritonitis and bacteraemia due to aeromonas hydrophila. this micro-organism is a gram-negative bacillus of the vibrionaceae family. it very frequently produces gastroenteritis in the human being. exceptionally it can give rise to extraintestinal infections, especially in immunodepressed patients. peritoniti ...200314716372
typing of clinical and environmental aeromonas sp. strains by random amplified polymorphic dna pcr, repetitive extragenic palindromic pcr, and enterobacterial repetitive intergenic consensus sequence pcr.a collection of 120 strains isolated from stool specimens collected from humans suffering from gastroenteritis and from environmental samples were analyzed by random amplified polymorphic dna pcr (rapd), repetitive extragenic palindromic pcr (rep-pcr), and enterobacterial repetitive intergenic consensus sequence pcr (eric-pcr). species of aeromonoas hydrophila, a. bestiarum, a. salmonicida, a. caviae, a. media, and a. veronii revealed clonal structure. there was no dominant clone causing gastroe ...200414715756
[biochemical characterization of aeromonad strains differing in pathogenicity].a virulent strain of motile aeromonad (77-18) and an avirulent strain (78-16) differed in the contents of lipids and phospholipids, odd fatty acids, activity of hydrolytic enzymes, and amount of proteins with molecular weights of 47-56 kda. it is assumed that proteins with molecular weights of 47-56 kda, proteolytic enzymes active within a wide ph range, and odd fatty acids may act as pathogenicity factors. each of these compounds or their combination determines certain stage of infection.200314714482
the antimicrobial activity of extracts of the lichen cetraria aculeata and its protolichesterinic acid constituent.in this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen cetraria aculeata has been investigated. the extracts were tested against twelve bacteria and eight fungi and found active against escherichia coli, staphylococcus aureus, aeromonas hydrophila, proteus vulgaris, streptococcus faecalis, bacillus cereus, bacillus subtilis, pseudomonas aeruginosa, listeria monocytogenes. no antimicrobial activity against the fungi was detected. it was determin ...200314713164
sequence analysis of amplified dna fragments containing the region encoding the putative lipase substrate-binding domain and genotyping of aeromonas hydrophila.dna fragments were amplified by pcr from all tested strains of aeromonas hydrophila, a. caviae, and a. sobria with primers designed based on sequence alignment of all lipase, phospholipase c, and phospholipase a1 genes and the cytotonic enterotoxin gene, all of which have been reported to have the consensus region of the putative lipase substrate-binding domain. all strains showed lipase activity, and all amplified dna fragments contained a nucleotide sequence corresponding to the substrate-bind ...200414711636
laribacter hongkongensis: a potential cause of infectious diarrhea.in this study, we describe the isolation of laribacter hongkongensis, a recently described genus and species of bacterium, in pure culture on charcoal cefoperazone deoxycholate agar from the stool of six patients with diarrhea. three patients were residents of hong kong, and three of switzerland. in none of the stool samples obtained from these six patients was salmonella, shigella, enterohemorrhagic escherichia coli, vibrio, aeromonas, plesiomonas, or campylobacter recovered. rotavirus antigen ...200314711474
aeromonas hydrophila and aspiration pneumonia: a diverse presentation.although there are ever increasing reports of extraintestinal human infections caused by aeromonads, in both immunocompromised and immunocompetent patients, respiratory tract infections remain uncommon. we describe a case of aspiration pneumonia in an immunocompetent patient with multiple sclerosis, caused by a community acquired, multidrug resistant strain of aeromonas hydrophila sensitive only to meropenem. the case highlights the clinical significance of aeromonas hydrophila as a respiratory ...200314703621
temperature and growth-phase effects on aeromonas hydrophila survival in natural seawater microcosms: role of protein synthesis and nucleic acid content on viable but temporarily nonculturable response.the behaviour of aeromonas hydrophila in nutrient-poor filter-sterilized seawater was investigated at 23 and 5 degrees c with respect to its growth phase. at both temperatures, the culturable a. hydrophila population declined below the detection level (0.1 c.f.u. ml(-1)) after 3-5 weeks, depending on the initial physiological state of the cells. during the first week, starved a. hydrophila cells appeared more resistant to the seawater stress at 5 degrees c than cells initially in the exponential ...200414702411
in vitro inhibition of microbial flora of fish by nisin and lactoperoxidase system.to test the antimicrobial effects of nisin and lactoperoxidase system (lp system) against sardines flora. this study is part of a programme designed to investigate the preservability of fish using these inhibitors as potential biopreservatives.200414687217
evaluation of novel beta-ribosidase substrates for the differentiation of gram-negative bacteria.to synthesize novel substrates for the detection of beta-ribosidase and assess their potential for the differentiation of gram-negative bacteria.200414678171
phylogenetic analysis and in situ identification of the intestinal microbial community of rainbow trout (oncorhynchus mykiss, walbaum).to identify the dominant culturable and nonculturable microbiota of rainbow trout intestine.200414678165
the immunosuppressive effect of alpha-permethrin on indian major carp, rohu (labeo rohita ham.).the immunosuppressive effects of bath exposure to a sub lethal concentration of the synthetic pyrethroid alpha-permethrin (3.05 x 10(-4) mg l(-1)) in the indian major carp, labeo rohita was studied after 45 days' exposure. in some groups, the effects of alpha-permethrin on non-specific defences and serum enzymes of carp were investigated after challenge with aeromonas hydrophila. several nonspecific immune responses and serum enzymes were reduced after exposure of alpha-permethrin. bactericidal ...200414675832
purification of antimicrobial factor from granules of channel catfish peripheral blood leucocytes.the channel catfish (ictalurus punctatus) is extensively used in aquaculture in the southeast us and is susceptible to many bacterial infections acquired from its pond environment. research is needed to better understand the defensive response and innate immunity of channel catfish against fish pathogens like edwardsiella ictaluri and aeromonas hydrophila. the main objectives were purification and characterization of an innate antimicrobial factor isolated from catfish leucocytes that has both b ...200414672693
[a successful treatment of progressive necrotizing myositis caused by aeromonas veronii biotype sobria].we report a 52 year-old patient with malignant lymphoma who developed necrotizing soft tissue infection in the left lower extremity by aeromonas veronii biotype sobria during a neutropenic period after intensive chemotherapy. we decided to amputate the involved leg when signs and symptoms of necrotizing myositis were progressing despite administration of sensitive antibiotics. he survived with subsequent intensive supportive care. it is to note that even though the patient's neutrophil count is ...200314672014
bacterial composition of the biofilm on the surface of course sediment of the danube: with special reference to the clinically important bacteria.on monthly intervals and over a period of 14 months (november, 1993-december, 1994) biofilm samples from sediments taken at the szentendre island on the danube were culturel and the isolated organisms were examined macromorphologically and micromorphogically and tested for oxidase and catalase production and their ability to oxidise and ferment glucose. the majority (85%) of the strains isolated were catalase positive. 43% were oxidase positive, 38% were glucose oxidisers and only 19% fermented ...199814666747
evaluation of two miniaturized systems, microscan w/a and bbl crystal e/nf, for identification of clinical isolates of aeromonas spp.fifty-two clinical strains and 22 type and reference aeromonas strains, previously genetically characterized by 16s rrna gene restriction fragment length polymorphism, were identified in parallel with the microscan walk/away and bbl crystal enteric/nonfermenter systems. the former identified only 14.8% of the isolates correctly, and the latter identified only 20.3% correctly, which indicates that neither of these systems is useful for this purpose.200314662969
reconfirmation of antimicrobial activity in the coelomic fluid of the earthworm eisenia fetida andrei by colorimetric assay.a novel tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2h-tetrazolium (mts) was used in the assessment of antimicrobial activity in earthworm in the presence of phenazine methosulphate (pms) as an electron coupling reagent. this activity was purified from the coelomic fluid of the earthworm (ecf), eisenia fetida andrei (oligochaeta, lumbricidae, annelids) using a series of column chromatography techniques and was tested against three gram-negative st ...200314660872
cloning, expression, and cell surface localization of paenibacillus sp. strain w-61 xylanase 5, a multidomain xylanase.we have shown that a xylan-degrading bacterium, w-61, excretes multiple xylanases, including xylanase 5 with a molecular mass of 140 kda. here, we emend the previously used classification of the bacterium (i.e., aeromonas caviae w-61) to paenibacillus sp. strain w-61 on the basis of the nucleotide sequence of the 16s rrna gene, and we clone and express the xyn5 gene encoding xylanase 5 (xyn5) in escherichia coli and study the subcellular localization of xyn5. xyn5 encodes 1,326 amino acid residu ...200314660338
viable cell detection by the combined use of fluorescent glucose and fluorescent glycine.the combined use of a fluorescent glucose (2nbdg) and a fluorescent glycine (nbd-gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several escherichia coli strains and coliforms. thirty-five out of 41 strains showed marked uptake of 2nbdg but 6 strains were not able to take in 2nbdg. five out of these 6 strains showed nbd-gly uptake.200314646209
normal ocular features, conjunctival microflora and intraocular pressure in the canadian beaver (castor canadensis).the aim of the study was to assess the ocular features, normal conjunctival bacterial and fungal flora, and intraocular pressure (iop) in the canadian beaver (castor canadensis).200314641823
epidemiologic and clinical characteristics of acute diarrhea with emphasis on entamoeba histolytica infections in preschool children in an urban slum of dhaka, bangladesh.the epidemiology, clinical features, nutritional status, and causative agents of diarrhea were studied in 289 bangladeshi children (147 boys and 142 girls) 2-5 years old. the use of improved diagnostic tests for amebiasis enabled for the first time analysis of the contribution of entamoeba histolytica to total diarrheal illness in this community setting. the average incidence rate of diarrhea was 1.8/child-year, and the average number of diarrheal days was 3.7 days/child-year over an average obs ...200314640500
extended-spectrum beta-lactamase tem-24 in an aeromonas clinical strain: acquisition from the prevalent enterobacter aerogenes clone in france. 200314638519
the csbx gene of azotobacter vinelandii encodes an mfs efflux pump required for catecholate siderophore export.the csbx gene of azotobacter vinelandii was regulated in an iron-repressible manner from a divergent promoter upstream of the catecholate siderophore biosynthesis (csb) operon and was predicted to encode an efflux pump of the major facilitator superfamily. other proteins that were most similar to csbx were encoded by genes found in the catecholate siderophore biosynthesis operons of aeromonas hydrophila and stigmatella aurantiaca. inactivation of csbx resulted in 57-100% decrease in the amount o ...200314638426
the c-terminal module of chi1 from aeromonas caviae cb101 has a function in substrate binding and hydrolysis.the chitinase gene chi1 of aeromonas caviae cb101 encodes an 865-amino-acid protein (with signal peptide) composed of four domains named from the n-terminal as an all-beta-sheet domain chin, a triosephosphate isomerase (tim) catalytic domain, a function-unknown a region, and a putative chitin-binding domain (chbd) composed of two repeated sequences. the n-terminal 563-amino-acid segment of chi1 (chi1deltaadeltachbd) shares 74% identity with chia of serratia marcescens. by the homology modeling m ...200314635132
chloramphenicol and florfenicol susceptibility of fish-pathogenic bacteria isolated in france: comparison of minimum inhibitory concentration, using recommended provisory standards for fish bacteria.to investigate the distribution of antimicrobial resistance to phenicols in the fish pathogenic bacteria aeromonas salmonicida, motile aeromonas, yersinia ruckeri, lactic bacteria and the nutritionally fastidious flavobacterium psychrophilum. the last species was screened on two media (diluted mueller-hinton and peptone-enriched anacker and ordal), both supplemented with horse serum.200314633029
molecular characterization and distribution of virulence-associated genes amongst aeromonas isolates from libya.the aim of the study was to type 52 aeromonas spp. isolates from chicken carcasses, children with diarrhoea and a hospital environment in libya, and to determine the distribution of putative virulence genes amongst them.200314633028
role of cys221 and asn116 in the zinc-binding sites of the aeromonas hydrophila metallo-beta-lactamase.the cpha metallo-beta-lactamase produced by aeromonas hydrophila exhibits two zinc-binding sites. maximum activity is obtained upon binding of one zinc ion, whereas binding of the second zinc ion results in a drastic decrease in the hydrolytic activity. in this study, we analyzed the role of asn116 and cys221, two residues of the active site. these residues were replaced by site-directed mutagenesis and the different mutants were characterized. the c221s and c221a mutants were seriously impaired ...200314625692
a simple method for semi-preparative-scale production and recovery of enterocin as-48 derived from enterococcus faecalis subsp. liquefaciens a-48-32.production of enterocin as-48 by enterococcus faecalis a-48-32 was compared between standard and high-cell density batch fermentations. in high-cell density cultures, bacteriocin production was 2.47-fold higher, provided that the ph was controlled during the fermentation. a two-step procedure for recovery of milligram quantities of purified bacteriocin was developed, based on adsorption of the bacteriocin on carboxymethyl sephadex cm-25 followed by reversed-phase chromatography on a semi-prepara ...200314607403
liver abscess and aeromonas bacteremia with septic pulmonary embolism.a 68-year-old healthy man was admitted because of liver abscess and aeromonas bacteremia accompanied by pulmonary embolism. aeromonas sobria was isolated from blood. coagulation parameters were normal and a lower limb venogram showed no evidence of thrombosis. septic embolism originating from the liver abscess was considered as the possible cause of pulmonary embolism. the patient was successfully treated with antibiotics.200314606725
duplex real-time sybr green pcr assays for detection of 17 species of food- or waterborne pathogens in stools.a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using the qiaamp dna stool mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. the protocol used the same lc-pcr with 20 pairs of specific primers. the products formed were identified based on a melting point temperature (t(m)) curve analysis. the 17 species of food- or waterborne pathogens examined were enteroinvasive escherichi ...200314605150
sampling natural viral communities from soil for culture-independent analyses.an essential first step in investigations of viruses in soil is the evaluation of viral recovery methods suitable for subsequent culture-independent analyses. in this study, four elution buffers (10% beef extract, 250 mm glycine buffer, 10 mm sodium pyrophosphate, and 1% potassium citrate) and three enumeration techniques (plaque assay, epifluorescence microscopy [efm], and transmission electron microscopy [tem]) were compared to determine the best method of extracting autochthonous bacteriophag ...200314602622
the adp-ribosylating toxin, aext, from aeromonas salmonicida subsp. salmonicida is translocated via a type iii secretion pathway.aext is an extracellular adp ribosyltransferase produced by the fish pathogen aeromonas salmonicida subsp. salmonicida. the protein is secreted by the bacterium via a recently identified type iii secretion system. in this study, we have identified a further 12 open reading frames that possess high homology to genes encoding both structural and regulatory components of the yersinia type iii secretion apparatus. using marker replacement mutagenesis of aopb, the a. salmonicida subsp. salmonicida ho ...200314594831
fatal bacteremic pneumonia caused by aeromonas hydrophila in a previously healthy child.aeromonas hydrophila sepsis and pneumonia are rare diseases in children that carry a high mortality rate. we report a case of fatal bacteremic pneumonia caused by a. hydrophila in a previously healthy 5-year-old child. the source of infection was not determined and the child died within 4 hours of admission. in children who develop a fulminant disease of pneumonia with or without sepsis, particularly those who have underlying medical conditions, a possible a. hydrophila infection, though rare, s ...200314582567
[post-traumatic ulcerations]. 200314576613
distribution of virulence genes in clinical and environmental isolates of aeromonas spp.the distribution and phenotypic activity of the genes encoding for serine protease, glycerophospholipid-cholesterol acyltransferase, lipases, aerolysin/hemolysin and dnases were investigated in 234 isolates identified by 16s rdna-rflp representing all the species of aeromonas. the former three genes were found to be highly conserved among the genus. aerolysin/hemolysin and dnase genes and beta-hemolytic activity were significantly more frequent in clinical than in environmental isolates. aerolys ...200314574104
the screening of hydrogen peroxide-producing lactic acid bacteria and their application to inactivating psychrotrophic food-borne pathogens.lactic acid bacteria were isolated from various food samples and evaluated for hydrogen peroxide (h2o2) production. cells suspended in 0.5% (wt/vol) glucose plus 0.5% (wt/vol) lactate (ph 7.0) were incubated for 5 h at 37 degrees c under aeration. among 193 strains, 27 strains accumulated 201-300 ppm h2o2, and 4 strains accumulated more than 301 ppm h2o2 in the cell suspensions. among the 9 high-level h2o2-producing strains, 8 strains were identified as lactococcus lactis subsp. lactis. the cell ...200314570275
bacterial enteropathogens isolated in childhood diarrhoea in kuala lumpur--the changing trend.a retrospective review of all stool samples obtained from children aged < 16 years with diarrhoea from university of malaya medical centre (ummc), kuala lumpur, from 1978 to 1997 was undertaken to ascertain the pattern of bacterial pathogens causing diarrhoea in children in an urban area in malaysia. of 26444 stool samples processed, 2989 (11%) were positive. the five most common bacterial pathogens isolated were non-typhoidal salmonella (57%), enteropathogenic e. coli (epec) (14%), shigella spp ...200214569714
[phenotypes of beta-lactam resistance in the genus aeromonas].this work aimed to investigate resistance profiles towards beta-lactam antibiotics in correlation with beta-lactamases production in the genus aeromonas. in a series of 417 wild-type strains, biochemical identification and testing with 11 beta-lactams by the disk-diffusion method revealed 5 predominant phenotypes: a. hydrophila complex/class b, c and d beta-lactamases; a. caviae complex/class c and d beta-lactamases; a. veronii complex/class b and d beta-lactamases; a. schubertii spp./class d be ...200314567197
aeromonas hydrophilia infections after penetrating foot trauma.the bacterium aeromonas hydrophila is an anaerobic gram-negative bacillus commonly found in natural bodies of water and can cause infection in patients who suffer water-associated trauma or in immunocompromised hosts. the authors present 5 cases of penetrating wound trauma that did not involve any aquatic environment and developed rapidly forming infections. all patients presented with severe pain, cellulitis, ascending lymphangitis, fever, and pain on range of motion of the joint near the traum ...200314566724
modifications to the fathead minnow (pimephales promelas) chronic test method to remove mortality due to pathogenic organisms.whole effluent toxicity testing is used to evaluate the discharge of materials that may be harmful to indigenous aquatic life. unlike most environmental analyses, receiving water (the water body into which the effluent is discharged) often is used as dilution water in whole effluent toxicity tests to simulate the aquatic environment into which the effluent is introduced. in approximately 26% of whole effluent toxicity tests conducted by wisconsin (usa) certified labs between 1988 and 1998, a pat ...200314552005
isolation and antimicrobial susceptibility of aeromonas salmonicida in rainbow trout (oncorhynchus mykiss) in turkey hatchery farms.three aeromonas salmonicida strains were isolated from the livers of 265 rainbow trouts sampled. the antibiotic susceptibility test results showed that a. salmonicida strains were susceptible to streptomycine and ciprofloxacin. however, they were resistant to amoxycilline + clavulanic acid, penicillin, erythromycine, oxytetracycline and cefuroxime sodium.200314535932
application of risk management to the production chain of intensively reared fish.principles of risk management, as a part of risk analysis, are described with respect to their application to the production chain of intensively reared fish. according to the outcomes of the fao/who expert consultation on the application of risk management (rome, 1997), there are four steps: risk evaluation, risk management option assessment, implementation of management decisions, and monitoring and review. there is a lack of information on risk assessment for farmed fish though, regarding bac ...200314535456
isolation of cytotoxic aeromonas spp. from food. 200314535416
[a case of endogenous aeromonas hydrophila endophthalmitis].aeromonas hydrophila(a. hydrophila) may cause septicemia in immunocompromised hosts, but endophthalmitis due to this agent is quite rare. to our knowledge, there have been no reports of a. hydrophila endophthalmitis in japan. we report a case of endogenous endophthalmitis caused by aeromonas hydrophila.200314531314
vaccine efficacy in spotted wolffish anarhichas minor: relationship to molecular variation in a-layer protein of atypical aeromonas salmonicida.atypical aeromonas salmonicida strains comprise a heterogeneous group in terms of molecular and phenotypic characteristics. they cause various conditions of ulcer diseases or atypical furunculosis and are being isolated in increasing number from various fish species and geographical areas. several marine fish species susceptible to atypical a. salmonicida, including spotted wolffish anarhichas minor o., are now being farmed and new vaccines may be needed. a commercial furunculosis vaccine for sa ...200314524499
[an autopsy case of sepsis due to aeromonas hydrophila with hepatic cirrhosis type b]. 200314524237
hpr6.6 protein mediates cell death from oxidative damage in mcf-7 human breast cancer cells.reactive oxygen species (ros) cause cell death and are associated with a variety of maladies, from trauma and infection to organ degeneration and cancer. cells mount a complex response to oxidative damage that includes signaling from transmembrane receptors and intracellular kinases. we have analyzed the response to oxidative damage in human breast cancer cells expressing the hpr6.6 (human membrane progesterone receptor) protein. although hpr6.6 is related to a putative progesterone-binding prot ...200314523988
purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli.an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ...200314522599
studies of aerolysin promoters from different aeromonas spp.aeromonas spp. have been related to food and waterborne diseases. the pore forming toxin aerolysin is regarded as the most important virulence factor in aeromonas food poisoning. in this work the aerolysin promoters from several aeromonas spp. strains have been sequenced, and divided into two sequence groups. further analyses of the promoters were carried out in a reporter-plasmid, pstina-ii. this plasmid was constructed as a hybrid of puc4k, pacyc184 and pkk232-8. we could conclude that our con ...200314521877
aeromonas hydrophila bacteremia presenting as non-traumatic acute osteomyelitis in a cirrhotic patient.aeromonas hydrophila is a low-virulence gram-negative bacillus. it has never been reported as a pathogen of non-traumatic acute osteomyelitis in a cirrhotic patient. herein, we reported on a case of decompensated liver cirrhosis with aeromonas hydrophila infection presenting as acute gastroenteritis and non-traumatic acute osteomyelitis. it has been shown that aeromonas bacteremia usually affects immunocompromised subjects, such as those with liver cirrhosis. non-traumatic acute osteomyelitis sh ...200314515976
childhood gastroenteritis due to aeromonas hydrophila in ibadan, nigeria.this is a prospective study spanning a period of six months where stools of 400 diarrhoeic children and 100 non-diarrhoeic children aged 0-5 years were screened for aeromonas hydrophila and other enteric pathogens. out of the 400 diarrhoeic stools only three (0.75%) were positive for aeromonas hydrophila while none was positive in the control group. no other enteric pathogen was isolated from these positive samples, indicating that aeromonas hydrophila is responsible for their diarrhoea. continu ...200114510117
analysis of 16s-23s intergenic spacer regions and rrn operon copy number of aeromonas culicicola mtcc 3249t.the 16s-23s intergenic spacer and 23s rrna gene sequence were determined for a. culicicola mtcc 3249t. ten different isr, indicative of ten rrn operons, were found in the strain that were grouped in three major types. of the three types, isr i was non-coding while isr ii and iii coded for trna(glu)(uuc). the trna(glu)(uuc) sequence was identical to that of e. coli. both isr i and ii were represented by three clones whereas four clones represented isr iii. the number of nucleotide differences bet ...200314509830
influence of temperature on the immune response of carp to lipopolysaccharide and whole cell aeromonas bestiarum vaccines.immunisation of carp, cyprinus carpio, with four different experimental vaccines for protection against a. bestiarum was tested. carp immunized with different antigens of a. bestiarum p1s, and kept at 18 degrees c responded immunologically by relatively high titers of antibodies. antibody production was inhibited at 12 degrees c.200314509353
use of human cdna microarrays for identification of differentially expressed genes in atlantic salmon liver during aeromonas salmonicida infection.commercially available human complementary dna microarrays were used to compare differential expression in the livers of atlantic salmon ( salmo salar) infected with aeromonas salmonicida and of healthy fish. complementary dna probes were prepared from total rna isolated from livers of control salmon and infected salmon by reverse transcription in the presence of (33)p-dctp and independently hybridized to human gene-filters gf211 microarrays. of the 4131 known genes on the microarray, 241 spots ...200314508654
collagenous colitis in children: clinicopathologic, microbiologic, and immunologic features. 200314508225
increased efficacy of immersion vaccination in fish with hyperosmotic pretreatment.immersion vaccination is common practice in aquaculture, because of its convenience for mass vaccination with sufficient protection. however, the mechanisms of antigen uptake and presentation, resulting in a protective immune response and the role of the innate immune system therein are largely unknown. the impact of immersion vaccination on fish physiology and on the ensuing innate and specific immune response was characterized with fluorescently labeled particulate and soluble model antigens. ...200314505897
induction of mitogenic signalling in the 1ln prostate cell line on exposure to submicromolar concentrations of cadmium+.cadmium exposure increases the risk of prostate cancer. we now describe the effects of cd2+ on signalling and proliferation in 1ln prostate cells. cd2+ increased [3h]thymidine uptake and cell number twofold. cd2+ elevated intracellular ip3, cytosolic-free ca2+, phosphorylated mek1/2, erk1/2, p38 mapk and jnk two- to threefold. increased pdk1 and phosphorylation of the 85-kda regulatory subunit of pi 3-kinase, akt and p70s6k were also observed. cd2+ treatment increased transcription factors nfkap ...200314499349
a study of certain heterotrophic polarly flagellate water bacteria: aeromonas, pseudomonas and comamonas. 196214483901
[on the problem of the significance of aeromonas strains in enteritis in infants]. 196114470523
[antibodies in human serums to suspensions of bacteria of the genus aeromonas]. 196114470517
fermentation reactions of pseudomonas caviae and its serological relationship to aeromonads.liu, pinghui v. (university of louisville school of medicine, louisville, ky.). fermentation reactions of pseudomonas caviae and its serological relationship to aeromonads. j. bacteriol. 83:750-753. 1962.-pseudomonas caviae was found to ferment carbohydrates instead of oxidizing them. this characteristic would seem to eliminate this organism from the genus pseudomonas and place it in the genus aeromonas. it was identified as a. liquefaciens, by its species-specific extracellular antigens and by ...196214465955
[incidence of aeromonas in drinking water]. 196214463376
[hemolysin studies with aeromonas strains]. 196014448624
active and passive immunization of certain salmonid fishes against aeromonas salmonicida. 196514346115
bacteriology of spoilage of fish muscle. 3. characterization of spoilers.a total of 807 bacterial isolates from fresh and spoiling fillets of english sole (parophrys vetulus) stored at 5 c were classified as to genus and tested for various biochemical activities, including the ability to spoil sterile muscle press juice at 5 c. production of off-odor, volatile reducing substances, and trimethylamine was used to estimate spoilage. it was found that (i) spoilers could be distinguished from nonspoilers on the basis of the juice spoilage test, (ii) differentiation betwee ...196514339271
[study of fumarate reductase in facultative anaerobic bacteria]. 196514323040
[apropos of a pathogen of a rana ridibunda disease in bulgaria]. 196414266483
aeromonas hydrophila as the etiologic agent in a case of severe gastroenteritis. 196414237401
[on antilipase formation (aeromonas hydrophila) in rabbits following adminstration of different vaccines]. 196414190314
lethal action by an acetobacter on yeasts. 196414190067
adansonian analysis and deoxyribonucleic acid base composition of some gram-negative bacteria.colwell, r. r. (georgetown university, washington, d.c.), and m. mandel. adansonian analysis and deoxyribonucleic acid base composition of some gram-negative bacteria. j. bacteriol. 87:1412-1422. 1964.-the deoxyribonucleic acid (dna) base compositions and s values for a minimum of 134 coded properties were determined for representative cultures of the genera pseudomonas, xanthomonas, aeromonas, vibrio, aerobacter, escherichia, alcaligenes, and flavobacterium. those cultures having a high degree ...196414188722
doehle bodies in bufo temporaria afflicted with 'red leg disease'. 196414187640
alteration of the gc patterns in human sera incubated with bacteria. 196414164895
spontaneous peritonitis and bacteremia in laennec's cirrhosis caused by enteric organisms. a relatively common but rarely recognized syndrome. 196414138877
an effective treatment for red-leg disease in rana pipiens. 196314134133
the onpg test in diagnostic bacteriology. comparison of the onpg test and the conventional lactose-fermentation test. 196414123350
[cross neutralization of the lipase in aeromonas hydrophila and salmonicida by antilipase; antigenic differences in the lipase of bacteria of the genera vibrio and aeromonas]. 196314121851
[neutralization of the enzyme activity and specific precipitation of lipase by antilipase]. 196314121850
the classification of 'bacterium salmonicida'. 196314121202
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