PMID(sorted descending)
pulsed-field gel electrophoresis profiles of aeromonads isolated from healthy and diseased helix aspersa from french snail farms.the xbai digestion patterns of chromosomal dna of 42 aeromonads isolated from french breeding snails during a new epizootic disease, which rapidly progressed to death during the summer of 1994, were analyzed by pulsed-field gel electrophoresis. biochemical identification to species level was also performed. interestingly, we found that 76% of the aeromonads isolated from diseased snails clustered into a unique pulsotype (p1) whatever their geographic origin, and were assessed to belong to aeromo ...200516391663
polar flagellum biogenesis in aeromonas hydrophila.mesophilic aeromonas spp. constitutively express a single polar flagellum that helps the bacteria move to more favorable environments and is an important virulence and colonization factor. certain strains can also produce multiple lateral flagella in semisolid media or over surfaces. we have previously reported 16 genes (flgn to flgl) that constitute region 1 of the aeromonas hydrophila ah-3 polar flagellum biogenesis gene clusters. we identified 39 new polar flagellum genes distributed in four ...200616385045
presence of aeromonas spp in water from drinking-water- and wastewater-treatment plants in mexico city.the frequency of aeromonas spp in three wastewater-treatment plants (wwtps) and two drinking-water plants (dwps) in méxico city was determined. samples were taken throughout a year by the moore's swab technique. a total of 144 samples were obtained from wwtps and 96 from dwps of both incoming and outflowing water. aeromonas spp was isolated in 31% of the samples, from both kinds of sources. the technique used for the isolation of the pathogen was suitable for samples with high associate microbio ...200516381156
the udp n-acetylgalactosamine 4-epimerase gene is essential for mesophilic aeromonas hydrophila serotype o34 virulence.mesophilic aeromonas hydrophila strains of serotype o34 typically express smooth lipopolysaccharide (lps) on their surface. a single mutation in the gene that codes for udp n-acetylgalactosamine 4-epimerase (gne) confers the o(-) phenotype (lps without o-antigen molecules) on a strain in serotypes o18 and o34, but not in serotypes o1 and o2. the gne gene is present in all the mesophilic aeromonas strains tested. no changes were observed for the lps core in a gne mutant from a. hydrophila strain ...200616369010
dna adenine methyltransferase influences the virulence of aeromonas hydrophila.among the various virulence factors produced by aeromonas hydrophila, a type ii secretion system (t2ss)-secreted cytotoxic enterotoxin (act) and the t3ss are crucial in the pathogenesis of aeromonas-associated infections. our laboratory molecularly characterized both act and the t3ss from a diarrheal isolate, ssu of a. hydrophila, and defined the role of some regulatory genes in modulating the biological effects of act. in this study, we cloned, sequenced, and expressed the dna adenine methyltra ...200616368997
spatial distribution of salmonella, escherichia coli o157:h7, and other bacterial populations in commercial and laboratory-scale sprouting mung bean beds.the reliability of testing spent irrigation water to assess the microbiological status of sprouting mung bean beds has been investigated. in commercial trials, the distribution of opportunistic contaminants within 32 bean sprout beds (25 kg of mung beans per bin) was assessed 48 h after germination. the prevalence of generic escherichia coli, thermotolerant coliforms, and aeromonas in sprouts (n = 288) was 5, 11, and 39%, respectively, and 57, 70, and 79% in the corresponding spent irrigation wa ...200516355820
effects of linear cationic alpha-helical antimicrobial peptides on immune-relevant genes in trout macrophages.there are increasing evidence of the potential role of antimicrobial peptides in the regulation of immune responses in mammalian species. however, the effects of these peptides in fish have yet to be investigated. in this study, we examined the transcriptional expression profile of representative immune-relevant genes in a trout macrophage cell line, rts11, in response to three linear cationic alpha-helical antimicrobial peptides (insect cecropin b, fish pleurocidin and a cecropin analogue cf17) ...200616352337
effect of gamma irradiation on the microflora of freshwater fish: ii. generic identification of aerobic bacteria from yellow perch fillets.studies on the generic identification of bacteria isolated from nonirradiated and irradiated (0.3 and 0.6 mrad) yellow perch fillets during the course of microbial spoilage have been conducted. after the enumeration and tabulation of macrocolonies on petri dish cultures obtained from fillets, isolates were examined and keyed out essentially according to modified morphological and biochemical protocols of shewan. identification was further confirmed through reference to bergey's manual. data obta ...196616349702
transfer in marine sediments of the naturally occurring plasmid pras1 encoding multiple antibiotic resistance.the results of microcosm experiments performed with the fish-pathogenic bacterium aeromonas salmonicida acting as a donor showed that promiscuous plasmid pras1, which encodes tetracycline resistance, is transferred at a high frequency in marine sediments even in the absence of a selective factor. the presence of oxytetracycline resulted in an increase in the transfer frequency compared with that of a microcosm to which no selective factor was added. transfer frequencies of 3.4 x 10 transconjugan ...199416349453
a simple and widely applicable method for preparing homogeneous and stable quality control samples in water microbiology.test strains suspended in skim milk, quickly frozen in dry ice-ethanol, and stored at - 70 degrees c can be used as quality control samples that are immediately available by quickly thawing at 37 degrees c. the samples remain homogeneous and stable for at least 1 year, except for aeromonas hydrophila, which decreases 20 to 30% in 1 year.199416349443
identification of aeromonas species isolated from freshwater fish with the microplate hybridization method.aeromonas isolates were obtained from the intestinal tracts of six species of cultured freshwater fish and identified on the basis of their genotypic and phenotypic characters. the microplate hybridization method could differentiate type strains of aeromonas species and related bacteria. dna-dna hybridization analysis showed that 65 aeromonad isolates were 72 to 100% related with either aeromonas caviae, aeromonas hydrophila, aeromonas jandaei, aeromonas sobria, or aeromonas veronii. as many as ...199416349363
siderophore-mediated iron sequestering by shewanella putrefaciens.the iron-sequestering abilities of 51 strains of shewanella putrefaciens isolated from different sources (fish, water, and warm-blooded animals) were assessed. thirty strains (60%) produced siderophores in heat-sterilized fish juice as determined by the chrome-azurol-s assay. all cultures were negative for the catechol-type siderophore, whereas 24 of the 30 siderophore-producing strains tested positive in the csáky test, indicating the production of siderophores of the hydroxamate type. sideroph ...199416349298
an outbreak of aeromonas hydrophila infection in turtles (pseudemis scripta).an outbreak of aeromonas hydrophila infection with a high rate of mortality (95%) in turtles (pseudemis scripta) in italy is reported. pure cultures of the pathogen were isolated from liver, lung, kidney, and heart specimens of the turtles. the a. hydrophila isolate was resistant to amoxicillin, ampicillin, cephalothin, and trimethoprim-sulfamethoxazole but was sensitive to a number of other antibiotics tested. the study indicates that pet turtles can act as reservoirs of this pathogen and may p ...199416349264
purification and characterization of aeromonas caviae me-1 xylanase v, which produces exclusively xylobiose from xylan.a xylanase, which produces exclusively xylobiose from oat spelt and birch xylans, was isolated from the culture medium of aeromonas caviae me-1. the enzyme (xylanase v) was purified by ammonium sulfate fractionation, hydrophobic interaction, and ion-exchange and gel filtration chromatographies. the homogeneity of the final preparation was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and agarose gel electrofocusing. the molecular mass and isoelectric point of the xyla ...199416349182
thermal inactivation of aeromonas hydrophila as affected by sodium chloride and ascorbic acid.the combined effects of sodium chloride (0, 1.0, 1.5, and 3.0%) and ascorbic acid (0, 1.0, and 2.0 mmol/liter) with mild heat (46 degrees c) on the survival of aeromonas hydrophila were evaluated. because of the nonlinear nature of the survivor curves obtained, several equations yielding an r (coefficient of multiple determination) of congruent with 1 were tested. the equation that most closely fit the curvature of the observed data set was a hyperbolic function. equation coefficients were combi ...199316349116
4-methylumbelliferyl-beta-n-acetylglucosaminide hydrolysis by a high-affinity enzyme, a putative marker of protozoan bacterivory.hydrolysis of an artificial fluorogenic substrate, 4-methylumbelliferyl-beta-n-acetylglucosaminide, has been studied in a monoculture predator-prey system with either a flagellate (bodo saltans) or a ciliate (cyclidium sp.) fed upon pure bacterial culture (aeromonas hydrophila or alcaligenes xylosoxidans). aeromonas hydrophila produced a low-affinity beta-n-acetylglucosaminidase-like enzyme (k(m), >>100 mumol liter) but alcaligenes xylosoxidans did not. inoculation of both bacterial strains with ...199316349049
indigenous bacteria in hemolymph and tissues of marine bivalves at low temperatures.hemolymph and soft tissues of pacific oysters (crassostrea gigas) kept in sand-filtered seawater at temperatures between 1 and 8 degrees c were normally found to contain bacteria, with viable counts (cfu) in hemolymph in the range 1.4 x 10 to 5.6 x 10 bacteria per ml. pseudomonas, alteromonas, vibrio, and aeromonas organisms dominated, with a smaller variety of morphologically different unidentified strains. hemolymph and soft tissues of horse mussels (modiolus modiolus), locally collected from ...199316348962
enumeration and characterization of bacterial colonists of a submersed aquatic plant, eurasian watermilfoil (myriophyllum spicatum l.).a simple procedure for enumerating and grouping the bacterial colonists of eurasian watermilfoil (myriophyllum spicatum l.) is described. colony characteristics of bacteria associated with m. spicatum were better defined and more stable on nutrient-poor, diluted nutrient broth agar than on high-nutrient media. acinetobacter, cytophaga, flavobacterium, moraxella, pseudomonas and/or alcaligenes, and vibrio/aeromonas spp., as well as two highly fastidious unidentified bacterial groups (gram-negativ ...199216348792
influence of solid surface, adhesive ability, and inoculum size on bacterial colonization in microcosm studies.microcosm studies were performed to evaluate the effect of solid surfaces, bacterial adhesive ability, and inoculum size on colonization success and persistence of pseudomonas fluorescens and xanthomonas maltophilia, each with a tn5 insertion that conferred resistance to kanamycin and streptomycin. two types of microcosms were used: (i) a simple system that was colonized by aeromonas hydrophila and a coryneform and (ii) a complex system produced from lake water enrichment cultures. simple microc ...199216348769
molecular cloning of the tryptophan operon from an aeromonas hydrophila freshwater isolate.a genomic library of aeromonas hydrophila f9 was constructed by using pbr322 as a vector. from that, two dna fragments (5.8 and 11.6 kb) were isolated containing genetic information to complement trpa and trpb defects (5.8-kb fragment) and to complement trpa, trpb, trpc, trpd, and trpe defects (11.6-kb fragment) in escherichia coli mutants. evidence of the existence of a secondary promoter is given.199216348662
evidence for bacterial chemotaxis to cyanobacteria from a radioassay technique.lyngbya birgei and aphanizomenon flos-aquae elicited a significant chemotactic attraction of aeromonas hydrophila compared with controls lacking cyanobacteria. there was a positive exponential relationship between biomass (chlorophyll a) of l. birgei and a. flos-aquae and chemotactic attraction of a. hydrophila. the assay equipment was simple and reliable and could be used to study bacterial chemotaxis in other species in situ.199116348544
mineralization of linear alkylbenzene sulfonate by a four-member aerobic bacterial consortium.a bacterial consortium capable of linear alkylbenzene sulfonate (las) mineralization under aerobic conditions was isolated from a chemostat inoculated with activated sludge. the consortium, designated kjb, consisted of four members, all of which were gram-negative, rod-shaped bacteria that grew in pairs and short chains. three isolates had biochemical properties characteristic of pseudomonas spp.; the fourth showed characteristics of the aeromonas spp. cell suspensions were grown together in min ...199116348496
purification and properties of beta-1, 4-xylanase from aeromonas caviae w-61.aeromonas caviae w-61, which was isolated from water samples at the faculty of agriculture, tohoku university, produced beta-1, 4-xylanase (1,4-beta-d-xylan xylanohydrolase; ec extracellularly. the xylanase was purified to homogeneity by using deae-sephadex a-50, cm-sephadex c-50, and sephadex g-100 column chromatographies. the molecular weight of the purified enzyme was estimated to be 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the isoelectric point of the enz ...199116348410
bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture.aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ...198916347937
electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water.[this corrects the article on p. 1343 in vol. 51.].198616347195
evaluation of different assay systems for identification of environmental aeromonas strains.[this corrects the article on p. 655 in vol. 51.].198616347094
evaluation of different assay systems for identification of environmental aeromonas strains.important biochemical reactions in conventional tests were compared with counterpart reactions in two multiple test systems, api-20e (analytab products, plainview, n.y.) and aeromonas hydrophila medium, to evaluate their accuracy for the identification of motile aeromonas spp. isolated from fish. in a total of 49 aeromonas spp. isolates and 10 a. hydrophila reference strains, false-negative or -positive reactions were detected in the voges-proskauer test, indole production, gelatinase activity, ...198616347025
temporal occurrence of vibrio species and aeromonas hydrophila in estuarine sediments.marine sediments were assayed for their concentration of vibrio spp. and aeromonas hydrophila over 1 year. a temporal variation was observed in which a. hydrophila, and to a lesser degree v. fluvialis, were found in the winter months, v. parahaemolyticus and v. vulnificus predominated in the spring and summer, with non-o-1 v. cholerae and v. alginolyticus detected in the late summer and fall. these organisms were found in greatest numbers in the top 5 cm of sediment, but were detectable down to ...198516346948
significance of extracellular protease for growth of a heterotrophic bacterium, aeromonas salmonicida.the role of protease produced by a heterotrophic bacterium during growth was investigated with aeromonas salmonicida, the pathogen of fish furunculosis, strain a-7301 and its protease-deficient mutant ntg-1 induced by mutagenesis. strain a-7301 produced extracellular protease in a mixed amino acid medium (composed of gly, ala, val, ile, leu, thr, ser, cys, met, phe, tyr, lys, arg, pro, his, try, asp, asn, glu, and gln at equal concentrations of 0.1 g/liter). its multiplication rate was limited b ...198516346900
starch-ampicillin agar for the quantitative detection of aeromonas hydrophila.interest in aeromonas hydrophila as a food-borne and human pathogen is increasing. isolation media from the clinical laboratory were evaluated for food use and either did not give quantitative recovery of a. hydrophila or did not permit ready differentiation of a. hydrophila from the background microflora. a new medium was developed which permitted quantitative recovery of a. hydrophila from foods. the medium consisted of phenol red agar base (difco laboratories), soluble starch (10 g/liter), an ...198516346899
predominant bacteria in an activated sludge reactor for the degradation of cutting fluids.for the first time, an activated sludge reactor, established for the degradation of cutting fluids, was examined for predominant bacteria. in addition, both total and viable numbers of bacteria in the reactor were determined so that the percentage of each predominant type in the total reactor population could be determined. three samples were studied, and a total of 15 genera were detected. in each sample, the genus pseudomonas and the genus microcyclus were present in high numbers. three other ...198316346426
isolation and partial characterization of two aeromonas hydrophila bacteriophages.two aeromonas hydrophila bacteriophages, aeh1 and aeh2, were isolated from sewage. both phages showed binal symmetry. the dimensions of a. hydrophila phages aeh1 and aeh2 differed from those of the other aeromonas phages. also, phage aeh2 was the largest aeromonas phage studied to date. phage aeh1 formed small, clear plaques, and phage aeh2 formed turbid plaques with clear centers. both phages were sensitive to chloroform treatment, being totally inactivated after treatment for 1 h at 60 degrees ...198316346300
isolation and characterization of ultramicrobacteria from a gulf coast estuary.viable bacteria were recovered from estuarine waters passed through a 0.2-mum polycarbonate membrane filter. the recovery method included the use of a dilute nutrient broth for primary enrichment followed by conditioning of the organism to a dilute nutrient solid medium. these bacteria were gram-negative rods and coccobacilli having an nacl requirement and, upon initial culturing, low nutritional requirements. in response to increased nutrient preparations, these microorganisms underwent an incr ...198216345964
diversity dynamics of marine bacteria studied by immunofluorescent staining on membrane filters.of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. the isolates were characterized as aeromonas sp., chromobacterium cf. lividum, vibrio sp., and two pseudomonas spp. specific antibodies were produced by immunization of rabbits, and bacterial cells were stained on black uni-pore membrane filters by an indirect immunofluorescent staining procedure. the method proved to be very specific and prac ...198216345920
external microflora of a marine wood-boring isopod.bacteria associated with the marine wood-boring isopod limnoria lignorum were enumerated by acridine orange epifluorescence microscopy and by plate counts on several media; the plate-viable bacteria were isolated and identified. similar procedures were followed to enumerate and identify bacteria associated with the wood substrate from which the isopods were collected and with the surrounding water from the isopod habitat. approximately 1.4 x 10 bacterial cells were associated with each individua ...198116345871
nonselectivity of rimler-shotts medium for aeromonas hydrophila in estuarine environments.thirty presumptive aeromonas hydrophila isolates were collected from estuarine sources using rimler-shotts media. many of the isolates, especially the strains from a low-salinity site, were also identified as a. hydrophila using the api 20e system and two differential techniques devised for the identification of a. hydrophila. all of the isolates, however, had deoxyribonucleic acid base compositions with guanine-plus-cytosine ratios between 40 and 49 mol%, which excludes these strains from the g ...198116345850
effects of pharmaceutical wastes on microbial populations in surface waters at the puerto rico dump site in the atlantic ocean.a series of cruises during 1979 and 1980 to the pharmaceutical dump site located 64 km north of arecibo, puerto rico, in the atlantic ocean, was carried out to evaluate effects of wastes on the ecology of the microflora of surface waters of the dump site. in addition to bacteriological monitoring of the waste plume created by the release of wastes from the disposal barge, stations along a series of transects, extending north from coastal waters through and beyond the dump site, were sampled. lar ...198116345752
growth of aeromonas hydrophila at low concentrations of substrates added to tap water.the ability of an aeromonas hydrophila isolate obtained from filtered river water to grow at low substrate concentrations was studied in batch experiments with tap water supplied with low concentrations of substrates. growth was assessed by colony count determinations. the isolate only multiplied in the used tap water (2 to 3 mg of dissolved organic carbon per liter) after the addition of a small amount of an assimilable carbon compound. d-glucose especially caused growth of the organism even at ...198016345582
effects of naturally occurring aquatic organic fractions on am uptake by scenedesmus obliquus (chlorophyceae) and aeromonas hydrophila (pseudomonadaceae).naturally occurring organics were extracted from water collected from skinface pond near aiken, s.c. organics were separated into four nominal diameter size fractions (i, >0.0183; ii, 0.0183 to 0.0032; iii, 0.0032 to 0.0009; iv, <0.0009 mum) by membrane ultrafiltration and introduced into scenedesmus obliquus and aeromonas hydrophila cultures to determine their effects on am availability for uptake. effects on am uptake were determined in actively growing s. obliquus cultures after 96 h of growt ...197716345193
partial purification and some properties of tyrosine phenol-lyase from aeromonas phenologenes atcc 29063.tyrosine phenol-lyase was purified 32-fold from aeromonas phenologenes atcc 29063, the organism that produces phenol in refrigerated haddock. the purification procedure included ammonium sulfate fractionation, protamine sulfate treatment, and column chromatography with sephadex g-200, diethyl-aminoethyl-cellulose, and hydroxyapatite. the enzyme was found to be thermally inactivated at temperatures above 40 degrees c. the optimum ph of the enzyme was found to be ph 8.5. the michaelis constants fo ...197716345185
aeromonas distribution and survival in a thermally altered lake.par pond is a thermally enriched monomictic southeastern lake which receives heated effluent from a production nuclear reactor. fish populations in the lake have lesions of epizooty from which aeromonas spp. are readily isolated. distribution and population densities of aeromonas in the water column were measured along an oxygen and temperature gradient as well as seasonally. greater population densities of aeromonas occurred below the oxygen chemocline when the lake was stratified. survival of ...197716345182
[identification and phylogenetic study of pathogenic bacteria causing ulcer disease of cultured turbot (scophthalmus maximus)].an outbreak of ulcer disease of the cultured turbot scophthalmus maximus l. occurred in an indoor farm in haiyang, shandong province in the sweltering august of 2004 and caused significant mortality. at the beginning, infected turbots displayed sluggish swimming and anorexia. several days later, the turbot eyes swelled, the fins and tail turned red, and the back gradually ulcerated. dissection of the moribund fish showed that the ulcerative gills paled, the liver becamed bloodshot, the kidney an ...200516342752
impact of non-legionella bacteria on the uptake and intracellular replication of legionella pneumophila in acanthamoeba castellanii and naegleria aquatic environments, legionella pneumophila survives, in association with other bacteria, within biofilms by multiplying in free-living amoebae. the precise mechanisms underlying several aspects of the uptake and intracellular replication of l. pneumophila in amoebae, especially in the presence of other bacteria, remain unknown. in the present study, we examined the competitive effect of selected non-legionella bacteria (escherichia coli, aeromonas hydrophila, flavobacterium breve, and pseud ...200516341636
macrolide inactivation gene cluster mpha-mrx-mphr adjacent to a class 1 integron in aeromonas hydrophila isolated from a diarrhoeic pig in characterize a multidrug-resistant aeromonas hydrophila isolate (cvm861) that possesses a high-level macrolide inactivation gene cluster (mpha-mrx-mphr), previously only reported in escherichia coli.200616339607
engulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, macrobrachium rosenbergii.haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. isolated haemocytes of m. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast debaryomyces hansenii, the bacteria aeromonas hydrophila or enterococcus faecium, or with latex beads at 25 degrees c for 2 h, followed by washing to remove free particles. at least ...200516336474
use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum).from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ...200516336470
development of a rapid identification method for aeromonas species by multiplex-pcr.existing biochemical methods cannot distinguish among some species of aeromonads, while genetic methods are labor intensive. in this study, primers were developed to three genes of aeromonas: lipase, elastase, and dna gyraseb. in addition, six previously described primer sets, five corresponding to species-specific signature regions of the 16s rrna gene from a. veronii, a. popoffii, a. caviae, a. jandaei, and a. schubertii, respectively, and one corresponding to a. hydrophila specific lipase (hy ...200516333335
production of acylated homoserine lactones by aeromonas and pseudomonas strains isolated from municipal activated sludge.up to now, the production and role of n-acyl homoserine lactones (ahls) in activated sludge have been poorly understood. in this study, cross-feeding assays with the reporter strains agrobacterium tumefaciens ntl4 and chromobacterium violaceum cv026 were used to investigate ahl signal production by municipal activated sludge samples. ahl signal production was consistently detected from municipal activated sludge when different samples were incubated on nutrient media. from one municipal activate ...200516333331
cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica.cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (cis). when chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. testing of the adsorption and desorption ability of cis using various microbes such as escherichia coli, aeromonas hydrophila, pseudomonas aeruginosa, bacillus subtilis, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, ...200516332887
determination of the viability of aeromonas hydrophila in different types of water by flow cytometry, and comparison with classical methods.the presence of aeromonas spp. in water can represent a risk for human health. therefore, it is important to know the physiological status of these bacteria and their survival in the environment. we studied the behavior of a strain of aeromonas hydrophila in river water, spring water, brackish water, mineral water, and chlorinated drinking water, which had different physical and chemical characteristics. the bacterial content was evaluated by spectrophotometric and plate count techniques. flow c ...200516332772
food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization.a modified fluorescence in situ hybridization (fish) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-proteobacteria -- aeromonas hydrophila and pseudomonas fluorescens, respectively) to grazing by three protists: the flagellates bodo saltans and goniomonas sp., and the ciliate cyclidium glaucoma. both flagellate species prefer ...200516329920
adjuvant effect of propolis on immunisation by inactivated aeromonas hydrophila in carp (carassius auratus gibelio). 200616325428
identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins.the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ...200516316188
transcriptome response following administration of a live bacterial vaccine in atlantic salmon (salmo salar).antibacterial responses have been studied in atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroa(-)) strain of aeromonas salmonicida known to elicit protective immunity. three tissues were studied for transcriptional changes, the liver, head kidney and the gill. rna was collected from fish 6, 12, 24 and 48 h following infection or at the same time points from fish injected with pbs as non-infected control. pcr-select cdna subtraction libraries were con ...200616313960
high dietary vitamin c affects growth, non-specific immune responses and disease resistance in asian catfish, clarias batrachus.studies were conducted to determine the immunomodulatory effects of high dietary ascorbic acid (vitamin c) on growth, serum concentration, non-specific immune response and disease resistance of a commercially important asian catfish, clarias batrachus. four practical diets were formulated to contain 0, 500, 1000 and 2000 mg ascorbic acid (aa) equivalent/kg diet, supplied as l-ascorbyl-2-polyphosphate (lapp) and were fed for 2, 4, 6 and 8 weeks. each diet was fed to triplicate groups of catfish w ...200516311902
etiology of childhood diarrhea in zliten, determine the etiological agents of diarrhea in children from a small semi-urban city in libya and the association of age, gender, seasonal variation, breast-feeding, source of water for drinking, or antibiotic use with the isolation of enteropathogens and whether such agents are community or hospital acquired.200516311662
structural studies of the core region of aeromonas salmonicida subsp. salmonicida lipopolysaccharide.the core oligosaccharide structure of the in vivo derived rough phenotype of aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, ce-ms and one- and two-dimensional nmr analyses and established as the following: [carbohydrate: see text] where r=alpha-d-galp-(1-->4)-beta-d-galpnac-(1--> or alpha-d-galp-(1--> (approx. ratio 4:3). comparative ce-ms analysis of a. salmonicida subsp. salmonicida core oligosaccharides from strains a449, 80204-1 and ...200616297894
carriage by the housefly (musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in misurata, libya.using standard microbiological procedures, bacteria that are potentially pathogenic to humans were isolated from 150 houseflies collected in the libyan city of misurata (50 flies each from the central hospital, streets and abattoir). salmonella spp., yersinia enterocolitica and edwardsiella tarda were isolated from flies collected on the streets and in the abattoir but not from those collected in the hospital. shigella sonnei was detected in just one fly, which was collected in the abattoir. of ...200516297293
prevalence of aeromonas in different clinical and water samples with special reference to gastroenteritis.a hospital based study was carried out in the department of microbiology, tribhuvan university teaching hospital, institute of medicine with the aim to initiate the isolation and identification of aeromonas spp. from the stool samples of gastroenteritic patients and to determine the prevalence of aeromonas spp. in other clinical samples and water. altogether 293 samples were investigated that include 172 stool samples, 60 pus/wound swabs, 20 body fluids and 41 water samples. the samples were col ...200416295748
the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita.the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ...200616293422
wound infections in tsunami survivors: a commentary.survivors of the december 2004 indian ocean tsunami sustained a variety of wound infections, ranging from common pathogens to rarely seen organisms. this article discusses the likely microbiology potentially seen in wound infections with exposure to freshwater, seawater, soil, faecal or other contamination, and attempts to provide an organising framework for choosing empiric antibiotics for such infections. therapy for less frequently encountered clinical entities is also discussed, including te ...200516284684
[effects of aeromonas hydrophila biofilm on the drug resistance].aeromonas hydrophila (ah) biofilm (bf) model in vitro was established and antibiotic effects of 11 antimicrobials on bf and free-cell(fc) bacteria were tested. after the ah j-1 on the surfaces of the silicon pieces was incubated for 7 days in tsb, the intact bf was formed by detection of agno3 solution staining. the fc bacteria were resistant to penicillin but susceptible to enrofloxacin and fulgram. their minimum bactericidal concentrations (mbcs) were 256 microg/ml, 0.03 microg/ml and 0.25 mic ...200316276926
[invasion of aeromonas hydrophila in cells involving signal transduction and cytoskeleton].invasion of aeromonas hydrophila strain ah j-1 isolated from diseased fish to cultured hep-2cells monolayer was evaluated by the recovery of gentamincin-resistant (gm') bacteria from triton x-100 cell lysates. the invasive efficiency (ie) could reach to 0.2% at 37 degrees c. in addition, potential signal transduction pathways that precede bacterial internalization were studied by using signal transduction inhibitors. bacterial internalization of ah j-1 involved microfilaments and protein tyrosin ...200316276909
[synthesis of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by aeromonas hydrophila wq and its molecular basis].aeromonas hydrophila wq isolated from lake water was found to be able to synthesize polyhydroxyalkanoates (pha) copolymer consisting of 3-hydroxybutyrate (hb) and 3-hydroxyhexanoate (hhx) (phbhhx). lauric acid was found to be the most suitable carbon source for cell growth and phbhhx accumulation. the bacteria accumulated 49% phbhhx containing 6% hhx in terms of cell dry weight when grown on lauric acid for 72 h. 42% phbhhx consisting of 14% hhx was obtained with 5 g/l glucose and 10 g/l lauric ...200316276907
diversity of denitrifying bacteria isolated from daejeon sewage treatment plant.the diversity of the denitrifying bacterial populations in daejeon sewage treatment plant was examined using a culture-dependent approach. of the three hundred and seventy six bacterial colonies selected randomly from agar plates, thirty-nine strains that showed denitrifying activity were selected and subjected to further analysis. according to the morphological and biochemical properties, the thirty nine isolates were divided into seven groups. this grouping was supported by an unweighted pair ...200516273028
microbiological evaluation of bottled non-carbonated ("still") water from domestic brands in greece.the microbiological quality of 1,527 samples of bottled non-carbonated ('still') mineral water, purchased from retail outlets and derived from 10 manufacturing companies in greece, was investigated during the period 1995-2003. applying the membrane filter technique, the aliquots of water samples (250 ml) were analyzed for the presence and enumeration of total coliforms, escherichia coli, enterococcus spp. and pseudomonas aeruginosa. also, aerobic bacteria were counted as heterotrophic plate coun ...200616271413
spectroscopic and thermodynamic characterization of the e151d and e151a altered leucine aminopeptidases from aeromonas proteolytica.previous kinetic characterization of the glutamate 151 (e151)-substituted forms of the leucine aminopeptidase from aeromonas proteolytica (vibrio proteolyticus; aap) has provided critical evidence that this residue functions as the general acid/base. the close proximity of similar glutamate residues to the bridging water/hydroxide of the dinuclear active sites of metalloenzymes (2.80 and 3.94 angstroms in carboxypeptidase g2 and 3.30 and 3.63 angstroms in aap), suggests it may also be involved i ...200516270998
putative exposed aromatic and hydroxyl residues on the surface of the n-terminal domains of chi1 from aeromonas caviae cb101 are essential for chitin binding and hydrolysis.chitinase chi1 of aeromonas caviae cb101 possesses chitin binding sites at both its n and c termini. four putative exposed residues aligned in a line on the surface of the n-terminal domains of chi1 were found to contribute to the enzyme-chitin binding and hydrolysis via site-directed mutagenesis. also, it was found that chi1 requires the cooperation of the n- and c-terminal domains to bind fully with crystalline and colloidal chitin.200516269803
recognition of individual genes in diverse microorganisms by cycling primed in situ amplification.cycling primed in situ amplification-fluorescent in situ hybridization (cprins-fish) was developed to recognize individual genes in a single bacterial cell. in cprins, the amplicon was long single-stranded dna and thus retained within the permeabilized microbial cells. fish with a multiply labeled fluorescent probe set enabled significant reduction in nonspecific background while maintaining high fluorescence signals of target bacteria. the ampicillin resistance gene in escherichia coli, chloram ...200516269764
aerobic bacterial flora of the nasal cavity in gulf of california sea lion (zalophus californianus) pups.nasal swab samples from clinically healthy california sea lions pups (zalophus californianus) from six different reproductive rookeries in the gulf of california were collected to determine the type and frequency of the representative aerobic bacterial microflora of their nasal mucosa. a total of 114 samples were examined and 100 bacterial isolates were identified and typified by microbiological and biochemical standard tests. fifty four isolates corresponded to gram positive bacteria (54%) and ...200516266850
modulation of juvenile brook trout (salvelinus fontinalis) cellular immune system after aeromonas salmonicida fish, the first line of defence against infectious microorganisms is based on non-specific cellular immune mechanisms (innate immunity). in this study, we measured the non-specific immune parameters (natural cytotoxic cells (ncc) activity, lymphoproliferation, percentage of phagocytosis and phagocytic activity) in brook trout (salvelinus fontinalis) infected by a virulent strain of aeromonas salmonicida. eight days post-infection, the mortality of infected fish reached 70%. a transient immuno ...200616263179
synthesis and evaluation of new quinazolone derivatives of nalidixic acid as potential antibacterial and antifungal continuation of our work on synthesis of biheterocycles carrying the biodynamic heterocyclic systems at position 3, a series of new nalidixic acid derivatives having quinazolones moiety were synthesised to achieve enhanced biological activity and wide spectrum of activity. nalidixic acid was first converted into its acid chloride using thionyl chloride as an acylating agent at laboratory temperature. later it was converted to methyl ester. nalidixoyl chloride formed vigorously reacts with met ...200616260068
immunostimulatory nature of beta-glucans and baker's yeast in gnotobiotic artemia challenge tests.the use of new preventive approaches such as immunostimulants to reduce stress and mortalities, to maintain good health of cultured organisms and to stimulate the non-specific defence mechanism, is becoming increasingly important in aquaculture. yet detailed analysis reveals that in most experiments the validity of some conclusions with respect to the benefit of immunostimulation is still doubtful, especially in invertebrates. the use of standardized trials under controlled rearing conditions, c ...200616257233
effect of sex ratio on the immune system of oreochromis mossambicus (peters).the social environment of fish has a crucial role to play on the immune system and hence on the overall health status. stressors of social origin such as dominance, subordination, and fight for mate have a major impact on the immune system of fish. the present study was designed with the objective of finding the effect of sex ratio of the population on the immune system of oreochromis mossambicus. groups of fish were maintained for 28 days in three different sex ratios i.e., (i) all-male (ii) al ...200616256305
the major aeromonas veronii outer membrane protein: gene cloning and sequence analysis.the gene encoding the major outer membrane protein (omp) from aeromonas veronii, omp38, was cloned and characterized. sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the n-terminal and the consensus sequence ala-x-ala (ala-asn-ala) as the signal peptidase i recognition site. the mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 da. the degree of iden ...200516252131
immunoreactive antigens of the outer membrane protein of aeromonas hydrophila, isolated from goldfish, carassius auratus (linn.).aeromonas hydrophila causes disease under stress conditions or in concert with infection by other pathogens in goldfish. sero-diagnostic and/or immunoprophylactic tools against aeromonas infection in goldfish are not available so far. the present study was undertaken to fractionate and characterise the outer membrane proteins (omp) of a. hydrophila and to identify suitable immunoreactive components. a total of 10 fractions were generated from crude omp antigens upon gel permeation and subsequent ...200616243540
aeromonas hydrophila exotoxin induces cytoplasmic vacuolation and cell death in vero cells.many organisms are able to cause cell vacuolation, but it is unclear if this can be considered a step of apoptosis or necrosis, or a distinct form of cell death. in this study vero cells were used to evaluate the relationship between vacuolation and cell death pattern caused by exotoxins produced by environmental strains of a. hydrophila. cell damage has been evaluated morphologically as well as biochemically. cytotoxic and vacuolating titres were strictly correlated and the vacuolation has to b ...200516240698
processing of xyne (110-kda) of aeromonas caviae me-1 to 72-kda xylanase in escherichia coli extracellular 72-kda xylanase from an escherichia coli transformant that harbored pxed30 carrying xyne of aeromonas caviae me-1 was purified by extraction following sds-page to electrophoretic homogeneity. the analysis of n-terminal 10 amino acid residues (gly-ala-arg-ala-gln-ala-ala-ala-asp-val) revealed a 72-kda product derived by the cleavage of gly26-gly27 at the n-terminal region of 110 kda xyne. the 72-kda xylanase from a. caviae me-1 was found to have the same sequence as that of the n ...200316233547
a cytoplasmic xylanase (xynx) of aeromonas caviae me-1 is released from the cytoplasm to the periplasm by osmotic downshock.aeromonas caviae me-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, samia cynthia pryeri. one of the xylanases produced by a. caviae me-1, xynx (38 kda, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. generally, xylanases are extracellular or cell surface enzymes. however, xynx is not exported to the extracellular fluid by a. caviae me-1 and an escherichia coli transformant ...200316233445
the processing of high-molecular-weight xylanase (xyne, 110 kda) from aeromonas caviae me-1 to 60-kda xylanase (xyne60) in escherichia coli and purification and characterization of xyne60.a xylanase gene (xyne) encoding xyne (110 kda) was cloned from a lambda phage genomic library of aeromonas caviae me-1 which is a multiple-xylanase-producing bacterium. upon nucleotide sequence analysis, we found that xyne comprises 2823 by and encodes a protein of 941 amino acid residues (104,153 da), which was similar to endo-beta-1,4-xylanases which are categorized to glycosyl hydrolase family 10. an escherichia coli transformant that harbored pxed30 carrying xyne produced 110-, 84-, 72-, and ...200316233373
expression of a gene encoding chitinase (pca 8 orf) from aeromonas sp. no. 10s-24 in escherichia coli and enzyme characterization.a gene encoding chitinase from aeromonas sp. no. 10s-24 was expressed using ptrc99a in escherichia coli jm 105 which yielded a 5-fold higher activity than when puc19 was used. three different truncated enzymes (sa-1, sa-2 and sa-3) were obtained after purification. their isoelectric points were 7.0, 6.9, and 6.7, respectively. the enzymes showed two optimum phs, 4.0 and 7.0, when incubated with ethylene glycol chitin as the substrate, and were stable over a wide ph range (3.0-9.0). the optimum t ...200116233047
isolation and characterization of aeromonas sp. b-5 capable of decolorizing various dyes.aeromonas sp. b-5, which has the ability to decolorize azo dyes, was isolated from soil. aeromonas sp. b-5 completely decolorized 100 mg/l of bordeaux s by reductive cleavage of azo bonds under static conditions in 24 h. though the decolorization of bordeaux s by aeromonas sp. b-5 was suppressed under shaking, rapid decolorization was observed when the culture was changed to static conditions after cultivation with shaking. the indigoid dye, acid blue 74, was decolorized by aeromonas sp. b-5 und ...200016232913
cloning, nucleotide sequence and expression of the beta-n-acetylglucosaminidase gene from aeromonas sp. no. 10s-24.the beta-glcnacase gene of aeromonas sp. no. 10s-24 was cloned and sequenced. the gene consisted of 2505 bp encoding 835 amino acid residues. the amino acid sequence of the gene shares sequence homology with beta-glcnacases of vibrio vulnificus (37.4%), serratia marcescens (36.4%), v. harveyi (36.4%), v. parahemolyticus (33.5%), alteromonas sp. strain o-7 (27.0%), and candida albicans (28.6%). the beta-glcnacase gene of aeromonas sp. no. 10s-24 was about 630-fold overexpressed in escherichia col ...200016232720
characterisation of atypical aeromonas salmonicida infection in arctic charr salvelinus alpinus and european grayling thymallus thymallus.cultured stocks of arctic charr salvelinus alpinus and european grayling thymallus thymallus are vulnerable to infection by achromogenic atypical aeromonas salmonicida (aas). in finland, natural stocks of both fish species have to be supported by restocking, and aas infection poses a threat to successful restocking because no preventive means are available. in this study, we analysed aas isolates from arctic charr and european grayling and from other sources genetically, and characterised the si ...200516231637
skin and soft-tissue infections among tsunami survivors in southern thailand.among 777 patients transferred to 4 hospitals in bangkok from southern thailand after the tsunami of 26 december 2004, there were 515 with skin and soft-tissue infections. the most common organisms isolated were aeromonas species (145 [22.6%] of 641 isolates from 305 patients). most isolates were susceptible to aminoglycosides, third- and fourth-generation cephalosporins, quinolones, and imipenem but were resistant to amoxicillin-clavulanate and first-generation cephalosporins.200516231248
a comparative immunopathological study of injection site reactions in salmonids following intraperitoneal injection with oil-adjuvanted adequate understanding of the inflammatory mechanisms following intraperitoneally-injected oil-adjuvanted vaccines in fish is required for the improvement of the quality of vaccines. we report the findings of a study in which the importance of neutrophils, macrophages, lymphocytes and mast cells (mcs) in early inflammatory reactions of atlantic salmon were examined in comparison with those of rainbow trout and grayling. the inflamed areas in the pylorus/pancreas area (injection site) were seq ...200616221512
antibiotic binding to monozinc cpha beta-lactamase from aeromonas hydropila: quantum mechanical/molecular mechanical and density functional theory studies.the active-site dynamics of apo cpha beta-lactamase from aeromonas hydropila and its complex with a beta-lactam antibiotic molecule (biapenem) are simulated using a quantum mechanical/molecular mechanical (qm/mm) method and density functional theory (dft). the quantum region in the qm/mm simulations, which includes the zn(ii) ion and its ligands, the antibiotic molecule, the catalytic water, and an active-site histidine residue, was treated using the self-consistent charge density functional tig ...200516220984
influence of high-m alginate on the growth and survival of atlantic cod (gadus morhua l.) and spotted wolffish (anarhichas minor olafsen) fry.atlantic cod and spotted wolffish fry were fed high-m alginate containing feed for 59 and 55 days, respectively. during this period the fry showed a higher specific growth rate compared to controls. uptake and distribution of alginate was studied by inclusion of the (125)i-labelled molecule in the feed. the stomach and intestine contained the highest amount while the kidney, liver and spleen contained some, indicating that the alginate was taken up by the gut and transported to internal organs. ...200616219475
characterization of aeromonads isolated from the river narmada, india.thirty isolates of aeromonas species were isolated from river narmada at jabalpur during the period of january 2002-december 2002. diversity of these fresh water isolates were determined by randomly amplified polymorphic dna (rapd) analysis. these environmental isolates were found to be positive for virulence factors, i.e. protease, amylase, lipase and dnase. isolates were also positive for beta-hemolytic activity. all aeromonas species were tested for antibiotic resistance patterns and were fou ...200516217927
atypical mri findings in canavan disease: a patient with a mild course.canavan disease is a severe, progressive leukodystrophy with an autosomal recessive inheritance, caused by aspartoacylase (aspa) deficiency. the characteristic mri features include diffuse, symmetrical white matter degeneration in the subcortical areas, with bilateral involvement of the globus pallidus. proton magnetic resonance spectroscopy of the brain shows an increase in the concentration of n-acetylaspartic acid (naa). the altered naa metabolism has been traced to mutations in the gene enco ...200516217711
comparison of elisa and pcr vis-à-vis cultural methods for detecting aeromonas spp. in foods of animal origin.the present study was conducted to assess the best method of the most commonly used methods for detection of aeromonads in foods of animal origin. with this objective an omp based indirect plate elisa and a duplex-pcr using primers targeting aerolysin gene and 16s rrna gene and yielding amplicons of 252 bp and 599 bp, respectively, were standardized. the standardized protocols and the conventional cultural method were then compared for their respective sensitivities and specificities for detecti ...200616216375
biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) from palm oil products in a wautersia eutropha mutant.palm kernel oil, palm olein, crude palm oil and palm acid oil were used for the synthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] by a mutant strain of wautersia eutropha (formerly ralstonia eutropha) harboring the aeromonas caviae polyhydroxyalkanoate (pha) synthase gene. palm kernel oil was an excellent carbon source for the production of cell biomass and p(3hb-co-3hhx). about 87% (w/w) of the cell dry weight as p(3hb-co-3hhx) was obtained using 5 g palm kernel oil/l ...200516215858
production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from gluconate and glucose by recombinant aeromonas hydrophila and pseudomonas putida.aeromonas hydrophila 4ak4 and pseudomonas putida gpp104 were genetically engineered to synthesize poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (phbhhx) using gluconate and glucose rather than fatty acids. a truncated tesa gene, encoding cytosolic thioesterase i of escherichia coli which catalyzes the conversion of acyl-acp into free fatty acids, was introduced into a. hydrophila 4ak4. when grown in gluconate, the recombinant a. hydrophila 4ak4 synthesized 10% (w/w) phbhhx containing 14% (mol/mo ...200516215853
purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated.bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ...200516212035
numerical analysis of electrophoretic periplasmic protein patterns of aeromonas sp. strains.a total of 103 strains of aeromonas spp. isolated from clinical and from environmental samples was compared by using sds-page of periplasmic proteins patterns. strains isolated from polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in hong-kong. aeromonas sp. strains did not show a tendency to cluster according to their origin. our results have demonstrated no species-specific periplasmic protein profiles. a significant protein electropho ...200516209101
population-based epidemiological study of infections caused by carbapenem-resistant pseudomonas aeruginosa in the calgary health region: importance of metallo-beta-lactamase (mbl)-producing strains.a study was conducted in the calgary health region between may 2002 and april 2004 to define the population-based epidemiological characteristics of infections caused by imipenem-resistant pseudomonas aeruginosa and to explore the clinical outcomes due to metallo- beta -lactamase (mbl)-producing and non-mbl-producing strains.200516206075
arge-encoded n-acetyl-l-ornithine deacetylase from escherichia coli contains a dinuclear metalloactive site.the catalytic and structural properties of the arge-encoded n-acetyl-l-ornithine deacetylase (arge) from escherichia coli were investigated. on the basis of kinetic and itc (isothermal titration calorimetry) data, zn(ii) binds to arge with kd values that differ by approximately 20 times. moreover, arge exhibits approximately 90% of its full catalytic activity upon addition of one metal ion. therefore, arge behaves similarly to the aminopeptidase from aeromonas proteolytica (aap) in that one meta ...200516201833
[detection of multiresistance aeromonas with tem type resistant genes in patients with cirrhosis].to study the status of beta-lactamase produced by multiresistant aeromonas selected from cirrhosis patients to provide reference for treatment and reduce resistance and control spreading.200516201471
granulomatous inflammation of salt glands in ducklings (anas platyrhynchos) associated with intralesional gram-negative bacteria.the "nasal glands" occur in many bird species and are powerful sodium ion-excretory organs. in ducks, they are located in supraorbital bony recesses. granulomatous inflammation of these glands occurs with an incidence of approximately 1% in ducklings (anas platyrhynchos), and is not associated with specific clinical symptoms. we investigated nine glands of eight animals with granulomas by gross pathology and histopathology, and compared results of bacteriology with 20 non-lesioned nasal glands. ...200516191707
hydrolytic activity of alpha-amylase in anaerobic digested sludge.hydrolysis is usually considered to be a rate-limiting step in anaerobic digestion. for improving anaerobic solid waste treatments, it is essential to elucidate the mechanism of hydrolysis. in this study, alpha-amylase, one of the hydrolytic enzymes, was investigated for the elucidation of more precise mechanism of hydrolysis. alpha-amylase activity of solid starch-degrading bacteria (sdb) was estimated through batch experiments with several different substrates and with distinction between cell ...200516180437
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