PMID(sorted descending)
development of a pcr protocol for the detection of aeromonas salmonicida in fish by amplification of the fsta (ferric siderophore receptor) gene.the aims of the study were to evaluate a new pcr protocol designed to detect aeromonas salmonicida in fish tissues and to develop a non-destructive method for the diagnosis of furunculosis. a set of primers (fer3, fer4), flanking a fragment of the fsta gene (coding for the ferric-siderophore receptor) was designed, showing to be sensitive and specific. when compared to pcr methods previously reported, the new protocol recognized all the 69 a. salmonicida strains evaluated, with no cross-reaction ...200818035507
establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ...200818034834
aeromonas sobria prostatitis and septic shock in a healthy man with chronic alcoholic consumption.prostate infection caused by aeromonas sobria has not been reported in the literature. we presented a 44-year-old healthy man with a habit of alcoholic consumption who was admitted because of primary a. sobria prostatitis with septicemia. the patient was successfully treated with intravenous ofloxacin and was uneventful after a half-year follow-up. based on our rare case, we suggest to clinical physicians that prostatitis might be considered in healthy male patients with a. sobria bacteremia and ...200718032845
combination of n149s and d171g mutations in aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis.aeromonas caviae polyhydroxyalkanoate synthase (phac(ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(r)-3-hydroxybutyrate-co-(r)-3-hydroxyhexanoate] [p(3hb-co-3hhx)]. in a previous study, two phac(ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (n149s) or aspartate 171 by glycine (d171g) were isolated as higher active enzymes by means of evolutionary engineering. in this study, ...200718031343
accumulation of silver(i) ion and diamine silver complex by aeromonas sh10 biomass.the biomass of aeromonas sh10 was proven to strongly absorb ag+ and [ag(nh3)2]+. the maximum uptake of [ag(nh3)2]+ was 0.23 g(ag) g(-1)(cell dry weight), higher than that of ag+. fourier transform infrared spectroscopy spectra analysis indicated that some organic groups, such as amide and ionized carboxyl in the cell wall, played an important role in the process of biosorption. after sh10 cells were suspended in the aqueous solution of [ag(nh3)2]+ under 60 degrees c for more than 12 h, [ag(nh3)2 ...200718025596
sensitive and rapid detection of aeromonas caviae in stool samples by loop-mediated isothermal this study, a loop-mediated isothermal amplification assay targeting the 16s-23s intergenic spacer regions (internal transcribed spacer) of aeromonas caviae was developed. eighteen reference strains and 109 clinical samples were analyzed. the results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.200818024054
dietary korean mistletoe enhances cellular non-specific immune responses and survival of japanese eel (anguilla japonica).the present study was performed to investigate the immunostimulatory effects of korean mistletoe extract (km-110; viscum album coloratum) on the non-specific immune response and protection against aeromonas hydrophila infection in japanese eel (anguilla japonica). eels were fed under 4 regimes, 0%, 0.1%, 0.5% and 1.0% km-110 mixed diet. on day 14 after feeding, 15 fish from each group were injected i.p. with live a. hydrophila (3 x 10(6)cfu) and the remaining unchallenged fish from each group we ...200818023593
[neutropenic enterocolitis in adult patients with acute leukemia or stem cell transplant recipients: study of 7 cases].neutropenic enterocolitis (ne) is a complication arising in neutropenic patients with acute leukemia or solid tumours while treated with intensive chemotherapy. the optimal therapeutic procedures have not been well established.200718005634
a distinct cause of necrotizing fasciitis: aeromonas veronii biovar sobria.aeromonas veronii biovar sobria (veronii var. sobria) has not been implicated in the pathogenesis of necrotizing fasciitis in north america.200717999586
drinking water microbiological survey of the northwestern state of sinaloa, mexico.a potable water survey, in two important municipalities of the state of sinaloa, mexico was conducted. culiacan, capital city of sinaloa and its neighboring municipality, navolato were selected to enumerate aeromonas hydrophila, escherichia coli, fecal and total coliforms, pseudomonas aeruginosa, and heterotrophic plate count bacteria from 100 households' taps. manganese; residual chlorine; ph; temperature and turbidity were also examined. overall, aeromonas hydrophila was not detected in any of ...200817998613
aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains.aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. a total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in são paulo (brazil), were analyzed for the presence of aeromonas. samples were concentrated by membrane filtration and enriched in apw. ada medium was used for aeromonas isolation and colonies were confirmed by biochemical characterization. strains isolated w ...200817998612
isolation of aeromonas salmonicida from sea lamprey (petromyzon marinus) with furuncle-like lesions in lake ontario.for the past six decades, parasitic sea lampreys (petromyzon marinus) have caused devastating losses to salmonid fisheries in the great lakes. to reduce the number of sea lampreys, the great lakes fishery commission began a large-scale program based on trapping male sea lampreys, sterilizing them, and releasing sterile males back into streams to compete with fertile males for spawning females. the transfer of lampreys among lakes can potentially lead to the transfer of various pathogens, and thi ...200717984256
proteomic analysis of rainbow trout (oncorhynchus mykiss, walbaum) serum after administration of probiotics in diets.the response of rainbow trout (oncorhynchus mykiss, walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (apr). proteomic analysis by two-dimensional electrophoresis (2d) concurrently with mass spectrometry was used to detect apr related proteins in rainbow trout serum following feeding with probiotics aeromonas sobria gc2 and bacillus sp. jb-1. three candidate proteins increased following use of gc2, and wer ...200817981340
febrile response to infection in the american alligator (alligator mississippiensis).temperature probes were inserted into the stomachs of juvenile american alligators (alligator mississippiensis) maintained outdoors at ambient fluctuating temperatures. internal body temperatures (t(b)) were measured every 15 min for two days, and then the alligators were injected with bacterial lipopolysaccharide (lps), pyrogen-free saline, or left untreated. alligators injected intraperitoneally with lps exhibited maximum t(b)s 2.6+/-1.1 degrees c and 3.5+/-1.2 degrees c higher than untreated ...200717977038
cloning, expression and characterization of aerolysin from aeromonas hydrophila in escherichia coli.aerolysin is a toxin (protein in nature) secreted by the strains of aeromonas spp. and plays an important role in the virulence of aeromonas strains. it has also found several applications such as for detection of glycosylphosphatidylinositol (gpi)-anchored proteins etc. a. hydrophila is a ubiquitous gram-negative bacterium which causes frequent harm to the aquaculture. to obtain a significant amount of recombinant aerolysin in the active form, in this study, we expressed the aerolysin in e. col ...200717970277
[factors of virulence associated with enteropathogenicity in strains of aeromonas spp. isolated from children with diarrhea in mérida, venezuela].the feces of 397 patients with acute diarrheal disease (add) and of other 121 patients without diarrea (control group) were studied in the state of mérida, venezuela, from june 1993 to december 1994. the genus aeromonas was identified in patients with add in 11.83% and in 5.78% of the patients from the control group. on studying the virulence factors described for aeromonas (enterotoxin, cytotoxin, hemaglutinins, cellular hydrofibrosity, and hemolytic activity) in the isolated strains, it was de ...200517966577
tracheobronchitis caused by aeromonas veronii biovar sobria after near-drowning.a 19-year-old man developed an acute tracheobronchitis shortly after having been rescued from a near-drowning in a river where previous investigations had demonstrated the presence of 500 c.f.u. ml(-1) of aeromonas sp. in the water. an isolate of aeromonas veronii biovar sobria was identified as the causative agent of the tracheobronchitis. the causality was supported by the massive growth of a. veronii in bronchial secretion, the presence of a type iii secretion system in the bacterial isolate, ...200717965361
analysis of type iii effector genes in typical and atypical aeromonas salmonicida. 200717958615
cloning and characterization of photobacterium damselae ssp. piscicida phospholipase: an enzyme that shows haemolytic activity.a phospholipase gene of photobacterium damselae ssp. piscicida (ppp) was cloned from a genomic library and its nucleotide sequence was determined. the open reading frame consisted of 1218 bp encoding a protein of 405 amino acids with a predicted molecular mass of 46 kda. the ppp had identities (53-55%) with phospholipase and haemolysin of vibrio spp., while it showed low identities (23-26%) with glycerophospholipid cholesterol acyltransferase of aeromonas spp. a recombinant ppp (rppp) with a his ...200717958612
luxri homologs are universally present in the genus aeromonas.aeromonas spp. have been regarded as "emerging pathogens". aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a phenomenon that might be regulated by quorum sensing. however, only two species of the genus are reported to possess the luxri quorum sensing gene homologs. the purpose of this study was to investigate if the luxri homologs are universally present in the aeromonas strains collected from various cu ...200717953777
bacillus subtilis ab1 controls aeromonas infection in rainbow trout (oncorhynchus mykiss, walbaum).to develop a probiotic with effectiveness against aeromonas sp., which was pathogenic to rainbow trout.200717953580
isolation and selection of bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental isolate, select and evaluate bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish.200717953558
salt in surroundings influences the production of serine protease into milieu by aeromonas sobria.previously we have shown that the open reading frame 2 protein (orf2 protein), which is encoded at the 3 ' end of serine protease of aeromonas sobria (asp), functions as a chaperone protein in periplasm in the production of asp. both proteins, asp and orf2 protein, associate in periplasm and orf2 protein helps asp to take an active form. asp which is dissociated from orf2 protein emerges in milieu . in this study, we examined the effect of sodium chloride (nacl) in medium on asp production by a. ...200717951986
prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, río tinto (sw, spain).the prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, río tinto (sw, spain), has been analysed through denaturing gradient gel electrophoresis (dgge), cloning of 16s rrna genes and fluorescence in situ hybridization (fish). the dominant species present in the macrofilaments were acidithiobacillus ferrooxidans, leptospirillum ferrooxidans and acidiphilium spp., which represented the three main bacterial genera found in the water column o ...200717950555
screening and characterization of a bioflocculant produced by aeromonas isolate the bioflocculant-producing bacteria from activated sludge and investigate the flocculating characteristics of the newly isolated bioflocculant.200717948760
first occurrence of an imp metallo-beta-lactamase in aeromonas caviae: imp-19 in an isolate from france.we describe the first imp metallo-beta-lactamase in aeromonas caviae: imp-19, which differed from imp-2 by a single amino acid change (arg to ala at position 38). bla(imp-19) was found within a class 1 integron located on a 35-kb plasmid. this is also the first description of an imp producer in france.200717938180
construction of a chimeric aminopeptidase by a combination of gene shuffling and mutagenesis.three chimeric genes were constructed by gene shuffling of aminopeptidases from aeromonas caviae and vibrio proteolyticus. although expressed chimeric enzymes formed inclusion bodies in escherichia coli, the introduction of two amino acid mutations into the chimeric genes by site-saturated mutagenesis and a random mutation on error-prone pcr resulted in solubilization of the chimeric enzyme. in addition, active chimeric enzyme showed a different thermostability and thermoactivity to parental enz ...200817934695
a comparison of antibiotic resistance integrons in cattle from separate beef meat production systems at compare antibiotic resistance integrons in cattle from three separate grass-fed, grain-fed and certified organic cattle production systems at slaughter.200817927756
on the identification of clinical aeromonas by a new restriction fragment length polymorphism of 16s rdna method. 200717916125
prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment investigate the presence and distribution of integron-carrying bacteria from a slaughterhouse wastewater treatment plant (wwtp).200717913715
antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent.the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ...200717913083
molecular characterization of class 1 integrons and antimicrobial resistance in aeromonas strains from foodborne outbreak-suspect samples and environmental sources in hundred thirty-three aeromonas spp. isolates were examined for multiple antibiotic resistance phenotypes and prevalence of class 1 integron sequences. twenty-four (18.0%) of these isolates contained class 1 integron. seven different class 1 integrons were found among 24strains, with a total of 10 different gene cassettes encoding for resistance to trimethoprim (dfr12 and dfr2d), aminoglycosides (aada1 and aada2), beta-lactam antibiotics (oxa2), chloramphenicol (catb3 and catb8), quaternary a ...200717908616
virulence potential and genetic diversity of aeromonas caviae, aeromonas veronii, and aeromonas hydrophila clinical isolates from mexico and spain: a comparative study.a comparative study of 109 aeromonas clinical isolates belonging to the 3 species most frequently isolated from patients with diarrhea in mexico and spain was performed to investigate the distribution of 3 prominent toxin genes and the gene encoding flagellin of lateral flagella; 4 well-established virulence factors in the genus aeromonas. the aerolysin-hemolysin toxin genes were the most prevalent, being present in 89% of the total isolates. the ast toxin gene was conspicuously absent from the ...200717898843
[changes in the profiles of causative agents and antibiotic resistance rate for spontaneous bacterial peritonitis: an analysis of cultured microorganisms in recent 12 years].the causative agents for spontaneous bacterial peritonitis (sbp) and antibiotic resistance rate vary according to the regions and time. this study evaluated the recent changes in the profiles of microorganisms and antibiotic resistance rate for the choice of effective antibiotics in treating sbp.200717898553
[microbiological characteristics of spontaneous bacterial peritonitis in korea]. 200717898547
interactions of clinical and environmental aeromonas isolates with caco-2 and ht29 intestinal epithelial cells.evaluation of adherence and invasion of aeromonas spp. to human colon carcinoma cell lines caco-2 and ht29 and assessment of cytotoxic activity.200717897383
evaluating virulence of waterborne and clinical aeromonas isolates using gene expression and mortality in neonatal mice followed by assessing cell culture's ability to predict virulence based on transcriptional assess the virulence of aeromonas spp. using two models, a neonatal mouse assay and a mouse intestinal cell culture.200717897183
disruption of the polyhydroxyalkanoate synthase gene in aeromonas hydrophila reduces its survival ability under stress conditions.aeromonas hydrophila 4ak4 produces phbhhx copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate. in this paper, the physiological functions of phbhhx on the bacterial survival ability were systematically studied using a phbhhx synthase phac-disrupted mutant termed a. hydrophila cq4 and its wild type a. hydrophila 4ak4. it was found that phbhhx synthesis in a. hydrophila 4ak4 provided improved resistance against environmental stress factors including heat and cold treatments, hydrog ...200717888005
genetic variations in aeromonas hydrophila isolates from clinical and environmental sources in thailand.aeromonas hydrophila, a widely distributed human pathogen causing a variety of diseases, can be isolated from clinical and environmental sources. analysis in thailand of 110 isolates of aeromonas hydrophila by randomly amplified polymorphic dna-pcr (rapd-pcr) revealed one specific rapd pattern group (g) that was associated only with strains from environmental sources. cytotoxic activity, adhesion to epithelial cells and exoenzyme secretions of a. hydrophila were also investigated. a comparison o ...200717883013
identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16s rrna gene sequencing.bacteria present in water samples taken on a weekly basis, from june 2004 through june 2005, from three streams, were cultured on coliscan easygel agar plates. colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16s rrna gene. a total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. of 175 dark blue/purple colonies presumed to be e. coli, sequence analysis indicated that 45 ...200717878554
phenoloxidase is an important component of the defense against aeromonas hydrophila infection in a crustacean, pacifastacus leniusculus.the melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, aeromonas hydrophila, in the freshwater crayfish, pacifastacus leniusculus. rna interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phago ...200717855335
prevalence of campylobacter jejuni and enteric bacterial pathogens among hospitalized hiv infected versus non-hiv infected patients with diarrhoea in southern india.a prevalence study on campylobacter jejuni and other enteric bacterial pathogens was carried out in 200 hiv infected and 200 non-hiv infected subjects with diarrhoeal symptoms at an aids hospital in southern india. diarrhoeal specimens were inoculated onto standard culture media as well as onto columbia and campylobacter blood agar media for c. jejuni isolation. all the c. jejuni isolates were tested for antimicrobial susceptibility using kirby-bauer's method. a significant difference in recover ...200717852888
molecular characterization of goose-type lysozyme homologue of large yellow croaker and its involvement in immune response induced by trivalent bacterial vaccine as an acute-phase protein.lysozyme acts as an innate immunity molecule against the invasion of bacterial pathogens. here, the cdna of a goose-type lysozyme (g-lysozyme) was cloned from large yellow croaker (pseudosciana crocea) by expressed sequence tags (est) and race-pcr techniques. the full-length cdna of large yellow croaker g-lysozyme (lycgl) is 716 nucleotides (nt) encoding a protein of 193 amino acids (aa), with a theoretical molecular weight of 21.3kda. the deduced lycgl possessed the typical structural features ...200717850883
the development of probiotics for the control of multiple bacterial diseases of rainbow trout, oncorhynchus mykiss (walbaum).jb-1 and gc2, which were equated with bacillus sp. and aeromonas sobria respectively, were recovered from the digestive tract of rainbow trout, oncorhynchus mykiss and ghost carp, cyprinus sp. respectively, and demonstrated effectiveness as probiotics for the control of infections caused by aeromonas salmonicida, lactococcus garvieae, streptococcus iniae, vibrio anguillarum, vibrio ordalii and yersinia ruckeri. when administered to rainbow trout (average weight = 12 g) for 14 days in feed dosed ...200717850573
inactivation of pseudomonas aeruginosa and aeromonas hydrophila by silver in tap water.this study was conducted to assess the efficacy of silver as a secondary disinfectant to replace or reduce the level of chlorine utilized in water distribution systems. pseudomonas aeruginosa and aeromonas hydrophila are opportunistic pathogens present in drinking water and have been associated with waterborne disease. after 8 hours of exposure to 100 microg/l of silver, there was a >6-log10 reduction in p. aeruginosa in tap water at room temperature at ph7 and a 5.55-log10 reduction in the pres ...200717849299
rapid detection of virulence factors of aeromonas isolated from a trout farm by hexaplex-pcr.the detection of virulence factors of aeromonas is a key component in determining potential pathogenicity because these factors act multifunctionally and multifactorially. in this study water samples were collected from a trout farm on a seasonal basis, and diseased fish and aeromonas species were isolated and identified. for rapid detection of six virulence factors of isolated aeromonas, a hexaplex-polymerase chain reaction (hexaplex-pcr) assay was used. the detected virulence factors include a ...200717846582
aeromonas hydrophila infection causing delayed vascular thrombosis in a nearly amputated finger.we discuss an unusual case of aeromonas hydrophila infection in a partially amputated digit from contaminated animal products resulting in delayed vascular thrombosis and amputation.200717826563
the early response of atlantic salmon (salmo salar) macrophages exposed in vitro to aeromonas salmonicida cultured in broth and in fish.aeromonas salmonicida is a fish pathogen that causes furunculosis. virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. it is known that a. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. therefore, in this study, the early macrophage respon ...200817825909
[preparation and characterization of the mab against vibrio harveyi].to prepare the mab against vibrio harveyi and analyse their biological properties.200717825231
microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates.during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ...200817728184
identification of a new hemolysin from diarrheal isolate ssu of aeromonas hydrophila.a clinical strain ssu of aeromonas hydrophila produces a potent cytotoxic enterotoxin (act) with cytotoxic, enterotoxic, and hemolytic activities. a new gene, which encoded a hemolysin of 439-amino acid residues with a molecular mass of 49 kda, was identified. this hemolysin (hlya) was detected based on the observation that the act gene minus mutant of a. hydrophila ssu still had residual hemolytic activity. the new hemolysin gene (hlya) was cloned, sequenced, and overexpressed in escherichia co ...200717725618
aeromonas veronii, a tributyltin (tbt)-degrading bacterium isolated from an estuarine environment, ria de aveiro in portugal.organotin compounds are used in a variety of industrial processes therefore their subsequent discharge into the environment is widespread. bacteria play an important role in biogeochemical transformations acting as natural decontamination agents. therefore, screening for tributyltin (tbt)-resistant and -degrading bacteria is relevant for the selection of isolates with decontamination ability of these polluted areas. with this purpose, 50 strains were isolated from sediment and water from ria de ...200717719084
antimicrobial properties of moderately halotolerant bacteria from cenotes of the yucatan peninsula.isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes.200717718841
aeromonas hydrophilia colitis mimicking ischaemic colitis in an elderly woman. 200717716361
replication of legionella pneumophila in floating biofilms.biofilms are a major source of human pathogenic legionella pneumophila in aquatic systems. in this study, we investigated the capacity of l. pneumophila to colonize floating biofilms and the impact of acanthamoeba castellanii on the replication of biofilm-associated legionella. biofilms were grown in petri dishes and consisted of aeromonas hydrophila, escherichia coli, flavobacterium breve, and pseudomonas aeruginosa. six hours following inoculation, legionella were detected in floating biofilms ...200717713816
function and molecular architecture of the yersinia injectisome tip quantitative immunoblot analyses and scanning transmission electron microscopy (stem), we determined that the needle of the yersinia enterocolitica e40 injectisome consists of 139 +/- 19 yscf subunits and that the tip complex is formed by three to five lcrv monomers. a pentamer represented the best fit for an atomic model of this complex. the n-terminal globular domain of lcrv forms the base of the tip complex, while the central globular domain forms the head. hybrids between lcrv and its ort ...200717697254
a case of aeromonas hydrophila enteritis in the course of ulcerative colitis.a 24-year-old man with a previous diagnosis of ulcerative pancolitis presented with severe malabsorption with watery diarrhea, malaise, and weight loss. physical examination revealed paleness, hypotension, tachycardia, edema, ascites, and left-sided pleural effusion. laboratory analysis revealed hypoalbuminemia and hypocalcemia. further examination revealed that malabsorption was related to aeromanas hydrophila infection. clinical improvement was observed upon oral ciprofloxacin treatment. no cl ...200717693233
aeromonas hydrophila ah-3 aext is an adp-ribosylating toxin secreted through the type iii secretion system.we cloned and sequenced an adp-ribosylating toxin (aext) from a mesophilic aeromonas hydrophila strain ah-3 with a type iii secretion system (t3ss). this toxin only showed homology, in genes and proteins, with the first half of a. salmonicida aext. the a. hydrophila aext showed adp-ribosyltransferase activity, translocation through the t3ss system, and this a. hydrophila t3ss system is inducible under calcium-depleted conditions. the a. hydrophila aext mutant showed a slight reduction in their v ...200817689917
haemato-immunological responses to dietary yeast rna, omega-3 fatty acid and beta-carotene in catla catla juveniles.a preliminary study with 60 days feeding was conducted to study the immunomodulatory role of different immunostimulants like beta-carotene, omega-3 fatty acid and yeast-rna in catla catla fingerlings. two hundred and sixty four fingerlings were randomly distributed into eight treatment groups with each of three replicates. eight isonitrogenous (crude protein 34.12-35.40%) and isocaloric (458.41-461.48 kcal/100g) purified diets were prepared with graded level of beta-carotene, omega-3 fatty acid ...200717681812
a comparison of ten usepa approved total coliform/e. coli tests.since 2002, the united states environmental protection agency (usepa) has approved ten enzyme-based total coliform and e. coli detection tests for examination of drinking water. these tests include: colilert, colilert-18, colisure, m-coli blue 24, readycult coliforms 100, chromocult, coliscan, e * colite, colitag and mi agar. the utility of the enzyme based test systems is based on both the ability of the test to detect the target organisms at low levels and the ability of the test system to sup ...200717674575
prevalence of bacterial pathogens in biofilms of drinking water distribution systems.water for human consumption is required to be free from any bacteria that might pose a health risk. the presence of biofilms in the drinking water distribution system may play a role in the presence of potential pathogens in the drinking water supply. ninety-five biofilm samples from various parts of south africa were tested for the presence of escherichia coli, aeromonas, pseudomonas, salmonella, shigella and vibrio spp. members of these genera were quantified by the three-tube most probable nu ...200717674571
isolation and identification of aeromonas from patients with acute diarrhoea in kolkata, india.isolation of diarrhoea causing aeromonas was carried out in the division of active surveillance, national institute of cholera and enteric diseases (niced), kolkata for a period of 12 months from january 1999 to december 1999. out of 602 stool samples collected from patients with acute diarrhoea admitted in infectious diseases (id) hospital, kolkata, 64 (10.6%) samples were identified positive for aeromonas as the pathogen. the different isolated and identified species from patients with acute d ...200117664830
mesophilic aeromonas udp-glucose pyrophosphorylase (galu) mutants show two types of lipopolysaccharide structures and reduced virulence.a mutation in galu that causes the lack of o34-antigen lipopolysaccharide (lps) in aeromonas hydrophila strain ah-3 was identified. it was proved that a. hydrophila galu is a udp-glucose pyrophosphorylase responsible for synthesis of udp-glucose from glucose 1-phosphate and utp. the galu mutant from this strain showed two types of lps structures, represented by two bands on lps gels. the first one (slow-migrating band in gels) corresponds to a rough strain having the complete core, with two sign ...200717660404
carbohydrate analysis and serological classification of typical and atypical isolates of aeromonas salmonicida: a rationale for the lipopolysaccharide-based classification of a. salmonicida.the cell envelope of aeromonas salmonicida contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of bacterial cell membrane. using a recently developed in-source fragmentation technique, we screened 39 typical and atypical isolates of a. salmonicida and established their o-chain polysaccharide structure by capillary electrophoresis-mass spectrometry (ce-ms), compositional and linkage analyses and comparison to the previously determined o-chain polysaccharide st ...200717658271
isolation and identification of aeromonas from patients with acute diarrhoea in kolkata, india. 200217657081
aeromonas species isolated from cases of acute gastroenteritis.a total of 67 aeromonas strains were isolated as the sole bacterial pathogen from 1485 patients with acute gastroenteritis. a. hydrophila (64.2%) was the predominant isolate followed by a. sobria (28.4%) and a.caviae (7.4%). majority of the isolates were sensitive to gentamicin, nalidixic acid but were resistant to ampicillin. minimum inhibitory concentration (mic) of resistant strains of aeromonas to ampicillin ranged from 80-1280 microg/ml.200217657045
aeromonas exoenzyme t of aeromonas salmonicida is a bifunctional protein that targets the host cytoskeleton.type iii protein secretion has been shown recently to be important in the virulence of the fish pathogen aeromonas salmonicida. the adp-ribosylating toxin aeromonas exoenzyme t (aext) is one effector protein targeted for secretion via this system. in this study, we identified muscular and nonmuscular actin as substrates of the adp-ribosylating activity of aext. furthermore, we show that aext also functions as a gtpase-activating protein (gap), displaying gap activity against monomeric gtpases of ...200717656370
bacterial communities of leachate from tire monofill disposal sites.the disposal of used automobile tires is problematic due to the large number generated each year. newer methods of tire disposal have been developed in which tires are shredded and used for construction materials or buried in dedicated tire monofill sites. in this study, the bacterial assemblage associated with aqueous leachate collected from tire monofill sites was examined and compared to the bacterial assemblage of reference streams. leachate from two tire monofill sites in ohio (usa) was col ...200717651788
enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (oncorhynchus mykiss).we analysed the effect of probiotic strains on the cellular and humoral immune responses of rainbow trout (oncorhynchus mykiss), and their capacity to prevent furunculosis during a challenge trial. probiotic strains (lactococcus lactis ssp. lactis clfp 100, leuconostoc mesenteroides clfp 196, and lactobacillus sakei clfp 202) were administered orally to fish for 2 weeks at 10(6) cfu g(-1) of feed. in comparison to untreated control fish, the phagocytic activity of head kidney leukocytes and the ...200717645738
replication of legionella pneumophila in biofilms of water distribution pipes.biofilms similar to those present in water distribution pipes of anthropogenic aquatic systems were simulated in a rotating annular reactor using a non-legionella community consisting of aeromonas hydrophila, escherichia coli, flavobacterium breve and pseudomonas aeruginosa. the impact of this community and acanthamoeba castellanii on the replication of legionella pneumophila was investigated. despite the presence of 10(7) non-legionella bacteria, culture and real-time polymerase chain reaction ...200917644359
further characterization of a type iii secretion system (t3ss) and of a new effector protein from a clinical isolate of aeromonas hydrophila--part i.a type iii secretion system (t3ss)-associated cytotoxin, aext, with adp-ribosyltransferase activity and homology to pseudomonas aeruginosa bifuncational toxins exot/s, was recently identified from a fish pathogen aeromonas salmonicida. in this study, we reported the molecular characterization of an aext-like toxin gene (designated as aexu) from a diarrheal isolate ssu of a. hydrophila. the aexu gene was 1539bp in length and encoded a protein of 512 amino acid (aa) residues. the nh(2)-terminus of ...200717644303
biochemical characteristics, serogroups, and virulence factors of aeromonas species isolated from cases of diarrhoea and domestic water samples in chennai.the objective of the present study was to delineate the differences between the clinical and environmental aeromonas species with respect to their biochemical characteristics, serogrouping and virulence factors, in order to find a phenotypic marker of enteropathogenicity.200317643034
vibrionaceae from cases of acute diarrhoea and their antimicrobial sensitivity pattern - a five year prospective study.over a five year period, stool samples were screened for vibrionaceae from cases of acute diarrhoea, to study their isolation rate and their antimicrobial sensitivity pattern. all the isolates were identified by standard laboratory techniques. a total of 323 species belonging to vibrionaceae were isolated from 4492 stool samples tested over five year period (1996-2000), giving a positivity rate of 7.2%. maximum isolation was during the months of may to august (62.5%). out of 323 isolates, vibrio ...200317643020
meningitis due to aeromonas hydrophila.a 3-month-old male child with the history of fever, of not sucking the breast and exhibiting\symptoms of meningitis was brought to the hospital for medical advice and was investigated. aeromonas hydrophila was isolated thrice from csf, blood samples as well as the water source.200417642732
[prevalence of the bacteria causing diarrea in a rosario hospital, argentina].bacterial agents causing diarrea in patients attended in a hospital of rosario, argentina. the frequency of bacterial agents causing diarrhea can vary in patients of different areas, and through the time. in an epidemiological surveillance we studied 304 patients with diarrheal diseases looking for salmonella enterica, shigella spp, campylobacter spp, aeromonas spp, yersinia enterocolitica, and diarrheogenic escherichia coli. c. jejuni was isolated in 30 patients (9,9 %), salmonella in 18 (5,9 % ...200617639815
virulence and antibiotic susceptibility of aeromonas spp. isolated from drinking water.aeromonas isolates from tap water, mineral water, and artesian well water were investigated for their ability to produce different potential virulence factors or markers such as hemolysins, cytotoxins, phospholipase, dnase, hydrophobicity and their ability to adhere to epithelial cells and to abiotic surfaces. the susceptibility to antibiotics of aeromonas isolates was also examined. majority of the isolates displayed hemolytic activity against sheep erythrocytes, while only 7 of the 23 aeromona ...200817636377
the immune response and protective efficacy of vaccination with oral microparticle aeromonas sobria vaccine in mice.the effect of an oral alginate microparticle aeromonas sobria vaccine on immune response and protection of mice was investigated. the formalin-killed cultures (fkc) of a. sobria strain z-1 treated with three methods were incorporated into alginate microparticles, and the encapsulated vaccines were orally used for immunizing mice. four weeks after immunization, the serum agglutinating antibody titers, bactericidal activity of leucocytes and relative percent survival (rps) of mice immunized orally ...200717630205
the immune response and protective efficacy of oral alginate microparticle aeromonas sobria vaccine in soft-shelled turtles (trionyx sinensis).aeromonas sobria is an important bacterial pathogen of soft-shelled turtles (trionyx sinensis). the effect of an oral alginate microparticle a. sobria vaccine (mav) on immune response and protection efficacy of soft-shelled turtles was investigated. the formalin-killed cultures of a. sobria strain z-1 (fkc) were incorporated into alginate microparticles, and the encapsulated vaccine was orally used for immunizing turtles. the serum agglutinating antibody titers, bactericidal activity of blood le ...200717628697
bilateral endogenous endophthalmitis caused by aeromonas hydrophila. 200717627288
detection of the causative agent of furunculusis, aeromonas salmonicida in salmonids of the krka this paper we describe the bacterial community associated with salmonids from the krka river. diversity analysis demonstrated that majority of the recovered bacteria were related to aeromonadaceae group. bacterial analysis also revealed the presence of shigella spp. and pseudomonas fluorescens. isolation of aeromonas salmonicida from trout, presents first isolation of this bacteria croatian rivers.200817624808
protonation state and substrate binding to b2 metallo-beta-lactamase cpha from aeromonas hydrofila.the zinc enzymes metallo beta-lactamases counteract the beneficial action of beta-lactam antibiotics against bacterial infections, by hydrolyzing their beta-lactam rings. to understand structure/function relationships on a representative member of this class, the b2 m beta l cpha, we have investigated the h-bond pattern at the zn enzymatic active site and substrate binding mode by molecular simulation methods. extensive qm calculations at the dft-blyp level on eleven models of the protein active ...200717623844
alternative host model to evaluate aeromonas virulence.bacterial virulence can only be assessed by confronting bacteria with a host. here, we present a new simple assay to evaluate aeromonas virulence, making use of dictyostelium amoebae as an alternative host model. this assay can be modulated to assess virulence of very different aeromonas species.200717616616
identification of aeromonas veronii genes required for colonization of the medicinal leech, hirudo verbana.most digestive tracts contain a complex consortium of beneficial microorganisms, making it challenging to tease apart the molecular interactions between symbiont and host. the digestive tract of hirudo verbana, the medicinal leech, is an ideal model system because it harbors a simple microbial community in the crop, comprising the genetically amenable aeromonas veronii and a rikenella-like bacterium. signature-tagged mutagenesis (stm) was used to identify genes required for digestive tract colon ...200717616592
pathogenic bacteria associated with oysters (crassostrea brasiliana) and estuarine water along the south coast of brazil.oysters and estuarine water samples were collected monthly, from june 1998 to march 1999, in the cananéia estuary, on the south coast of são paulo, brazil, and analyzed for bacterial hazards with and without depuration in filtered estuarine water. aeromonas spp., plesiomonas shigelloides, vibrio cholerae o1, vibrio parahaemolyticus, and vibrio vulnificus were counted in oyster samples using the most probable number (mpn) and their presence verified in the surrounding estuarine water samples. the ...200717613090
specificity and durability of antibody responses in atlantic cod (gadus morhua l.) immunised with vibrio anguillarum o2b. 200717604648
convenient test using a combination of chelating agents for detection of metallo-beta-lactamases in the clinical laboratory.although transmissible metallo-beta-lactamases (mbls) are a serious threat to beta-lactam antibiotic therapy, the clsi currently does not recommend testing methods for the detection of mbls. the aim of this study was to evaluate the capability of double-disk tests (ddts) by using disks containing a combination of the chelators 2-mercaptopropionic acid (mpa) and tris-edta (te) to detect mbls. sixteen isolates (4 acinetobacter baumannii isolates, 6 pseudomonas aeruginosa isolates, 1 serratia marce ...200717596358
etiology of bacterial diarrhea in a major referral center in saudi arabia.a total of 34, 856 stool specimens from 19, 437 patients with symptoms of gastroenteritis were analyzed for bacterial enteropathogens during the period of january 1, 1985, to december 31, 1989, at a major tertiary care referral hospital in saudi arabia. bacterial pathogens were isolate from 1426 (7.3%) patients, with salmonella being the most frequent (3.8%), followed by campylobacter (2.0%), shigella (1.1%), and aeromonas hydrophila (0.34%). salmonella serogroups b and c and shigella b and d co ...199117590814
spontaneous bacterial peritonitis due to aeromonas hydrophilia. 199117590777
ci-990 (pd 131112): a new quinolone vitro susceptibility of 1021 strains of recent clinical isolates was determined against the new fluoroquinolone ci-990 (pd 131112) and compared with ci-960, ciprofloxacin, fleroxacin, lomefloxacin, norfloxacin, perfloxacin, sparfloxacin, and temafloxacin. the minimum inhibitory concentrations (mic) of ci-990 in mg/l required for >90% isolates were 0.03-0.5 for members of enterobacteriaceae, 0.12-2.0 for pseudomonas aeruginosa, 0.03-1.0 for aeromonas hydrophilia, 1.0-2.0 for acinetobacter calc ...199317588022
biological characterization of a new type iii secretion system effector from a clinical isolate of aeromonas hydrophila-part ii.we recently identified a novel type iii secretion system (t3ss) effector, aexu, from a diarrheal isolate ssu of aeromonas hydrophila, and demonstrated that mice infected with the deltaaexu mutant were significantly protected from mortality. although the nh(2)-terminal domain of this toxin exhibits homology to aext of a. salmonicida, a fish pathogen, and exot/s of pseudomonas aeruginosa, the cooh-terminal domain of aexu is unique, with no homology to any known proteins in the ncbi database. in th ...200717582731
recombinant prostate-specific antigen proaerolysin shows selective protease sensitivity and cell cytotoxicity.native proaerolysin is a channel-forming bacterial protoxin that binds to cell-surface receptors and then is activated by furin or furin-like proteases. we genetically engineered proaerolysin by replacing the furin-cleavage sequence with a prostate-specific antigen-selective sequence. the recombinant modified proaerolysin was expressed and purified from aeromonas salmonicida in good yields and purity. recombinant modified proaerolysin had no furin sensitivity and markedly increased prostate-spec ...200717581303
diagnostic potentials of the activities of lectins with whole cells of three bacterial diarrheagenic agents: aeromonas, plesiomonas, campylobacter.the reactiviteisofstrains ofplesionmona shigeloides, campylobater jejuni, campylobacter coli and aeromonas species with some lectins namely soyabean agglutinin (sba), ricinus communis agglutinin (rca), peanut agllutinin (pna), cancanavalin-a (con-a), lima bean agglutinin (lma) and abrin were investigated using the microtitre plate method. the results obtained show that all stains of a. hydrophila, a. caviae, a. sobria and p. shigelloides reacted very strongly with pna and sba. all strains of c. ...199817581001
controversial data on the association of aeromonas with diarrhoea in a recent hong kong study. 200717577068
x-ray crystallographic characterization of the co(ii)-substituted tris-bound form of the aminopeptidase from aeromonas proteolytica.the x-ray crystal structure of the co(ii)-loaded form of the aminopeptidase from aeromonas proteolytica ([coco(aap)]) was solved to 2.2a resolution. [coco(aap)] folds into an alpha/beta globular domain with a twisted beta-sheet hydrophobic core sandwiched between alpha-helices, identical to [znzn(aap)]. co(ii) binding to aap does not introduce any major conformational changes to the overall protein structure and the amino acid residues ligated to the dicobalt(ii) cluster in [coco(aap)] are the s ...200717574677
the role of c-di-gmp signaling in an aeromonas veronii biovar sobria strain.aeromonas is a ubiquitous gram-negative bacterium that persists in the environment. it is shown that all isolates of persistent aeromonas clones show strong biofilm formation ability. c-di-gmp regulates biofilm formation in many bacteria. to investigate the impact of c-di-gmp signaling, we introduced heterologous ggdef and eal domain proteins from salmonella typhimurium to an aeromonas veronii biovar sobria strain. overexpression of the ggdef domain protein adra increased c-di-gmp concentration ...200717573931
characterization of aeromonas caviae and a. veronii by standardized cellular protein electrophoretic patterns.aeromonas caviae and a. veronii recovered from clinical and environmental samples were characterized by sds-page of whole cell proteins. all strains were typable and showed unique banding patterns. a high intra-specific diversity within these two microorganisms was revealed. separation of the polish strains from the hong-kong isolates was observed. a. caviae and a. veronii were not distinguishable with respect to their ecological origin. however, strains with very similar protein patterns could ...200717571798
[isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba].extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ...200717554439
biochemical and functional characterization of udp-galactose 4-epimerase from aeromonas hydrophila.bacteria of genus aeromonas, responsible for a variety of pathological conditions in humans and fish, are ubiquitous waterborne bacteria. aeromonas produces several virulent factors including a complex of lipopolysaccharide and surface array protein, involved in colonization. udp-galactose 4-epimerase (gale) catalyzes the production of udp-galactose, a precursor for lipopolysaccharide biosynthesis, and thus is an important drug target. gale exhibits interspecies variation and heterogeneity at it ...200717553760
bacteriostatic effect of flavonoids isolated from leaves of psidium guajava on fish pathogens.the antimicrobial activity against fish bacterial pathogens of flavonoids (morin, morin-3-o-lyxoside, morin-3-o-arabinoside, quercetin, and quercetin-3-o-arabinoside) isolated from the leaves of psidium guajava was evaluated. the flavonoids were shown to have bacteriostatic effect on all of the tested bacteria.200717553634
use of the novel phylogenetic marker dnaj and dna-dna hybridization to clarify interrelationships within the genus aeromonas.the interrelationships of 27 aeromonas strains were investigated using dnaj sequences and dna-dna hybridization. dnaj sequence similarities showed a stronger relationship with dna-dna relatedness values than did 16s rrna gene sequence similarities. additionally, dnaj sequence analysis, with interspecies divergence over 5.2 % in most cases, gave better resolution than 16s rrna gene sequences for the differentiation of strains at the species level. relationships among aeromonas species were theref ...200717551035
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