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bondage, dominance, irrigation, and aeromonas hydrophila: california dreamin'. 19892911156
aeromonas hydrophila colitis presenting as medically refractory inflammatory bowel disease. 19892912037
phenotypic characterization and dna relatedness in human fecal isolates of aeromonas spp.phenotypic characteristics were used to identify 189 aeromonas strains isolated from human feces. one hundred forty-two of these strains were placed in 11 dna hybridization groups, and the genetic and phenotypic data were compared. according to the criteria of popoff, 66% of the strains were identified as aeromonas caviae, 18% were identified as a. sobria, and 16% were identified as a. hydrophila. some biochemical characteristics differed from the criteria of popoff; 19 of 40 (48%) of tested str ...19892913025
three-dimensional structure of an open form of the surface layer from the fish pathogen aeromonas salmonicida.cell-free culture supernatants of a lipopolysaccharide (lps) o-polysaccharide-deficient, single-insertion transposon mutant of the tetragonal surface protein array (s layer)-containing fish pathogen aeromonas salmonicida were examined by electron microscopy. negative staining showed that the s layer was released as sheets of tetragonal material, indicating that although surface retention of assembled s layer requires the presence of wild-type lps oligosaccharides, initial assembly of s-layer sub ...19892914846
growth of aeromonas spp. on butzler campylobacter selective agar and evaluation of the agar for the primary isolation of aeromonas spp. from clinical specimens.the fortuitous finding that aeromonas spp. grew well on butzler campylobacter selective medium (bcsa) in a microaerobic atmosphere at 42 degrees c prompted us to evaluate the performance of bcsa for selective isolation of aeromonads in comparison with ampicillin (30 micrograms/ml) sheep blood agar (asba30). overall recovery rates of aeromonads from 563 stool samples from patients with acute diarrhea were higher on asba30 (70.4%) than on bcsa (56.3%); however, 21 (29.5%) grew only on bcsa. the th ...19892915030
isolation of aeromonas hydrophila from a captive caracal lynx (felis caracal).aeromonas hydrophila was isolated from the internal organs of a captive caracal lynx (felis caracal) which died of acute septicemia. grossly, patchy areas of focal necrosis were found in the lungs, liver and kidney; there was ulceration in the stomach and intestines. microscopically, lesions contained cellular debris, neutrophils, lymphocytes and gram-negative bacilli. this is the first report of isolation of aeromonas hydrophila from a captive wild animal in nigeria.19892915393
synthesis of glycoconjugates derived from various lipopolysaccharides of the vibrionaceae family.conjugation of simple ketoses (such as 3-deoxy-d-manno-2-octulosonic acid and n-acetylneuraminic acid) and of various o-specific polysaccharides (from aeromonas hydrophila and aeromonas salmonicida) to the bifunctional spacer 1,6-hexanediamine, was achieved by reductive amination. the saccharide--1-(6-amino)-hexane alkyamines obtained were converted into the corresponding isothiocyanate derivatives and coupled to the free epsilon-amino group of lysine residues of the protein carrier bovine serum ...19892920731
purification and chemical characterization of a cholera toxin-cross-reactive cytolytic enterotoxin produced by a human isolate of aeromonas hydrophila.a bacterial protein toxin possessing hemolytic, enterotoxic, and cytotoxic activities as well as cross-reactivity to cholera toxin was purified from culture filtrates of a human diarrheal isolate of aeromonas hydrophila (ssu). this cytolytic enterotoxin was purified by ammonium sulfate precipitation, hydrophobic chromatography using phenyl-sepharose, anion-exchange chromatography on deae-bio-gel a, and size-exclusion high-performance liquid chromatography. the factor was a single polypeptide wit ...19892925244
bioactivity and immunological characterization of a cholera toxin-cross-reactive cytolytic enterotoxin from aeromonas hydrophila.a cytolytic enterotoxin of molecular weight 52,000 was isolated and purified from culture supernatants of a human diarrheal isolate (ssu) of aeromonas hydrophila. the toxin reacted with cholera antitoxin when tested in an enzyme-linked immunosorbent assay and by western blot (immunoblot) analysis. the appearance of cytotoxic and hemolytic activities in culture supernatant occurred simultaneously 8 h after the initial inoculation of the culture. loss of hemolytic activity and cholera toxin cross- ...19892925245
segmental colitis associated with aeromonas hydrophila.we describe a 40-yr-old woman who presented with abdominal pain and diarrhea, and who was found to have segmental colitis involving the cecum and ascending colon. aeromonas hydrophila was isolated from the stool, and her symptoms responded to appropriate antibiotic therapy. follow-up colonoscopy confirmed complete endoscopic and histologic resolution of her colitis. this report further supports the belief that aeromonas species are pathogens in healthy humans. this organism needs to be considere ...19892929569
the suicide phenomenon in motile aeromonads.certain strains of motile aeromonas species, including all those of aeromonas caviae examined, were shown to be suicidal. when they were grown in the presence of glucose at both 30 and 37 degrees c, there was rapid die-off of the organisms after 12 h of incubation, and viable cells generally could not be recovered after 24 h. it was shown that this phenomenon was due to the production of relatively high levels of acetic acid by these strains, even during growth under highly aerobic conditions, a ...19892930167
the comparative in-vitro activity of pefloxacin.the in-vitro antibacterial activities of pefloxacin, other 4-quinolones and representative beta-lactams and aminoglycosides were assessed by determination of minimum inhibitory concentrations (mics). pefloxacin (mics mostly 0.03-2 mg/l) was highly active against enterobacteriaceae. gentamicin had slightly lower activity, and ceftazidime and norfloxacin similar activities to pefloxacin whereas ciprofloxacin was more active. pefloxacin (mics 0.03-2 mg/l) was active against acinetobacter but again ...19862940213
in-vitro activity of newer quinolones against aerobic bacteria.nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ...19862940214
the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens.the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ...19852941257
electrostatic mechanism of survival of virulent aeromonas salmonicida strains in river water.the ecological mechanism of survival of aeromonas salmonicida, the bacterial pathogen of fish furunculosis, in river water was investigated by laboratory-based experiments with two virulent strains (which were autoagglutinating) and two virulent strains (which were nonagglutinating). a difference in net electrical charge of a. salmonicida cells was detected by electrophoresis; cells of the virulent strains were negative, whereas cells of the avirulent strains were positive. despite the loss of v ...19862942106
applicability of coagglutination test for detecting aeromonas salmonicida antigens from extracts of bacterial cultures and experimentally infected tissues of the common carp (cyprinus carpio l.). 19862953200
[hospital infections in relation to the use of leeches]. 19872954135
[aeromonas hydrophila infection of the scalp. peculiarities]. 19872955380
immunoglobulin binding by the regular surface array of aeromonas salmonicida.the cell surface of aeromonas salmonicida is covered by a regular surface array composed of a single species of protein, the a-protein (phipps, b. m., trust, t. j., ishiguro, e. e., and kay, w. w. (1983) biochemistry 22, 2934-2939). the array, known as the a-layer, is the key virulence factor for this organism. cells containing the a-layer specifically bound rabbit igg and human igm with high affinity (kd = 1.0 x 10(-6) m and 3.3 x 10(-6) m, respectively), but neither isogenic a-protein-deficien ...19882967820
virulence properties and enterotoxin production of aeromonas strains isolated from fish.the biological activities in vivo and in vitro of 59 motile aeromonas spp. isolated from fish and water tanks were simultaneously analyzed in poikilothermic and homoiothermic systems. a total of 64.3% of the isolates tested were pathogenic for fish, and 62% of aeromonas hydrophila and a. sobria isolates either virulent or nonvirulent for fish were enterotoxigenic. although the majority of the strains were proteolytic and amylolytic and produced dnase, other activities, such as elastase and staph ...19882972627
effect of an activated sludge wastewater treatment plant on ambient air densities of aerosols containing bacteria and viruses.bacteria- and virus-containing aerosols were studied during the late summer and fall seasons in a midwestern suburb of the united states before and during the start-up and operation of an unenclosed activated sludge wastewater treatment plant. the study showed that the air in this suburban area contained low-level densities of indicator microorganisms. after the plant began operating, the densities of total aerobic bacteria-containing particles, standard plate count bacteria, total coliforms, fe ...19852988442
synthesis, export, and assembly of aeromonas salmonicida a-layer analyzed by transposon mutagenesis.suicide plasmid pjb4ji, containing transposon tn5 and phage mu, was introduced into aeromonas salmonicida 449 which produces a surface protein array known as the a-layer. kanamycin-resistant exconjugants of 449 with altered ability to produce the a-layer were selected by virtue of their altered colonial morphology and color on medium containing the dye congo red. analysis of culture supernatants, periplasmic shock fluid, outer membranes, and whole-cell lysates by sodium dodecyl sulfate-polyacryl ...19852993248
molecular cloning and expression of a xylanase gene of alkalophilic aeromonas sp. no. 212 in escherichia coli.a gene coding for a xylanase activity of alkalophilic aeromonas sp. no. 212 (atcc 31085) was cloned in escherichia coli hb101 with pbr322. plasmid pax1 was isolated from transformants producing xylanase, and the xylanase gene was located in a 6.0 kb hind iii fragment. the pax1-encoded xylanase activity in e. coli hb101 was about 80 times higher than that of xylanase l in alkalophilic aeromonas sp. no. 212. about 40% of the enzyme activity was observed in the periplasmic space of e. coli hb101. t ...19852999298
[epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics].diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ...19853000920
nuclease s1 analysis of eubacterial 5s rrna secondary structure.single-strand-specific nuclease s1 was employed as a structural probe to confirm locations of unpaired nucleotide bases in 5s rrnas purified from prokaryotic species of rrna superfamily i. limited nuclease s1 digests of 3'- and 5'-end-labeled [32p]5s rrnas were electrophoresed in parallel with reference endoribonuclease digests on thin sequencing gels. nuclease s1 primary hydrolysis patterns were comparable for 5s rrnas prepared from all 11 species examined in this study. the locations of base-p ...19853001324
infections of the gastrointestinal tract in the immunocompromised patient.immunocompromised patients are susceptible to a variety of serious gastrointestinal infections. among the most common or most severe are mycobacterium avium-intracellulare, herpes simplex, candidiasis, cryptosporidiosis, and salmonellosis. some of these infections are difficult or impossible to treat successfully. the correct diagnosis often can only be established if it is first suspected and the appropriate studies are performed.19863010805
pathogens that cause travelers' diarrhea in latin america and africa.with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ...19863014633
comparative activities of the beta-lactamase inhibitors ytr 830, clavulanate, and sulbactam combined with ampicillin and broad-spectrum penicillins against defined beta-lactamase-producing aerobic gram-negative bacilli.the in vitro synergistic activities of the beta-lactamase inhibitors ytr 830, clavulanate, and sulbactam, combined with ampicillin, ticarcillin, mezlocillin, azlocillin, piperacillin, and apalcillin, were determined against 34 strains of members of the enterobacteriaceae family, pseudomonas aeruginosa, aeromonas hydrophila, and haemophilus influenzae with characterized plasmid or chromosomal beta-lactamases or both. strains were tested against fixed concentrations of beta-lactamase inhibitors (8 ...19863015017
a spectral study of cobalt(ii)-substituted bacillus cereus phospholipase c.the coordination sphere of both the structural and catalytic zinc ions of bacillus cereus phospholipase c has been probed by substitution of cobalt(ii) for zinc and investigation of the resultant derivatives by a variety of spectroscopic techniques. the electronic absorption, circular dichroic, magnetic circular dichroic, and electron paramagnetic resonance spectra were found to be strikingly similar when cobalt(ii) was substituted into either site and are consistent with a distorted octahedral ...19863019384
molecular cloning and expression in escherichia coli of the structural gene for the hemolytic toxin aerolysin from aeromonas hydrophila.the structural gene for the hemolytic toxin aerolysin has been cloned into the plasmid vectors pbr322 and pembl8+. the gene was localized on the hybrid plasmids by analysis of plasmids generated by transposon mutagenesis. the sequence of the first 683 bases of an insert in pembl8+ was determined and shown to encode the amino terminus of the protein as well as a typical signal sequence of 23 amino acids. aerolysin is produced by e. coli cells containing the cloned aerolysin gene and it is process ...19863020368
[diarrhea caused by aeromonas in ivory coast].the authors drawn epidemiological, clinical conclusions, and the geographical data concerning the diarrheas with aeromonas in ivory-coast. the results relate to a study realized from january 1982 to july 1985. out of the 1,594 excrements 627 germes have been isolated and among which 153 strains of aeromonas if for instance 24.4% of the whole germs. aeromonas caviae is the most frequent species (50%) compared to 27.3% for aeromonas sobria, and 22.7% for aeromonas hydrophila.19863026671
the characterization of a conjugative r-plasmid isolated from aeromonas salmonicida.we have analyzed two related r-plasmids isolated from aeromonas salmonicida: par32 and pja8102-1, which encode resistance to chloramphenicol, streptomycin, and sulfonamides (su). the genetic map of pja8102-1 indicated that this plasmid consists of at least three functionally different regions. one is the region conferring ability to transfer (tra), one is the region coding for drug resistance genes (r-det), and one is the region encoding the replication functions (rep). both plasmids contain rep ...19863027727
construction of a transposon containing a gene for polygalacturonate trans-eliminase from klebsiella oxytoca.a dna fragment containing a klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon tn5. this new transposon, designated tn5-pga+, had a transposition frequency of 1 x 10(-6). the broad host range plasmid pr751::tn5-pga+ was conjugally transferred to a variety of genetic backgrounds. the ability to degrade polygalacturonate was expressed in aeromonas hydrophila, alcaligenes eutrophus, azotomonas insolita, escherichia coli, pseudomonas put ...19873034186
a new tetracycline-resistance determinant, class e, isolated from enterobacteriaceae.a fifth tetracycline(tc)-resistance determinant, designated class e, has been identified on a transferable plasmid found in a fecal strain of escherichia coli. this determinant does not show homology by dna-dna hybridization at high stringency with any of four other tc resistance determinants (classes a, b, c and d) previously described among the enterobacteriaceae. resistance is inducible by 1 microgram tc/ml and increases the minimum inhibitory concentration 130-fold for tc and 3.5-fold for mi ...19863034728
two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines.the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ...19873038946
cloning of the gene for the surface array protein of aeromonas salmonicida and evidence linking loss of expression with genetic deletion.a gene bank of dna from the fish pathogenic bacterium aeromonas salmonicida was constructed in the bacteriophage lambda gt11. phage lambda gt11/10g, a recombinant carrying a 4.0-kilobase fragment of a. salmonicida dna, was found to express the surface array protein (a protein) in escherichia coli. sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the protein expressed from the cloned gene had a subunit molecular weight of 49,000, which was identical to that of subunits in the ...19873040676
hospital-associated infection from leeches. 19883044210
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...19883045003
phenotypic and genotypic characterization of tetracycline- and oxytetracycline-resistant aeromonas hydrophila from cultured channel catfish (ictalurus punctatus) and their environments.oxytetracycline-resistant (otcr) and tetracycline-resistant (tcr) aeromonas hydrophila were isolated commonly from catfish intestinal contents and the water and sediment of catfish culture ponds, but less frequently in market catfish. isolates demonstrated two resistance phenotypes, tcr otcr and tcs otcr, when plated directly on to macconkey agar containing 30 micrograms of tetracycline or oxytetracycline per ml. tcs otcr isolates expressed tcr after induction by 1 h of growth in tryptic soy bro ...19883046493
mesophilic aeromonads in human disease: current taxonomy, laboratory identification, and infectious disease spectrum.the importance of the genus aeromonas in human disease has recently become better appreciated through the use of improved methodologies for the recovery and identification of aeromonads from biologic specimens and as a result of medical studies aimed at determining the clinical significance of these bacteria when isolated from localized and invasive infections of humans. taxonomic schemes currently allow for the identification of six aeromonas species (five mesophilic and one psychrophilic), fou ...19883055195
the production of a macrophage-activating factor from rainbow trout salmo gairdneri leucocytes.rainbow trout head kidney and blood leucocytes are shown to be capable of secreting a soluble macrophage-activating factor (maf) after stimulation with concanavalin a (con a). the presence of phorbol myristate acetate (pma) as a co-stimulant increased the production of maf. both respiratory burst activity (nitroblue tetrazolium, nbt, reduction and h2o2 production) and bactericidal activity were enhanced after incubation of resident or elicited macrophages with the maf-containing supernatants for ...19883056854
differential susceptibility of salmonid fishes to furunculosis correlates with differential serum enhancement of aeromonas salmonicida extracellular protease activity.rainbow trout, atlantic salmon and brown trout, respectively, exhibit an increasing order of susceptibility to furunculosis. we investigated if this may be due to a differential sensitivity to exotoxins or ability to inhibit the exoprotease which is required by the bacterium to digest protein for growth. differences were slight in susceptibility to exotoxins and serum inhibition of exoprotease at high exotoxin:serum ratios. however, at low ratios brown trout serum markedly enhanced protease acti ...19883059141
aeromonas and plesiomonas as etiological agents. 19883059998
bacterial-induced diarrhoea.in diverse parts of the world, many bacterial strains have been recognised as the causative agents of diarrhoea. great achievements have been made in perceiving the varied mechanisms which explain their intestinal pathogenicity. these achievements include the elucidation of the 5 mechanisms of disease production by escherichia coli, the mechanisms of action of shigella, salmonella, campylobacter and yersinia, new information about antimicrobial-associated colitis caused by clostridium difficile ...19883069445
expression of met-(-1) angiogenin in escherichia coli: conversion to the authentic less than glu-1 protein.a method for obtaining authentic human angiogenin utilizing an escherichia coli recombinant expression system is described. a synthetic gene encoding angiogenin was placed into a vector for direct expression under the control of a modified e. coli trp promoter. the protein was produced by the bacteria in an insoluble form and purified to homogeneity by cation-exchange and reversed-phase hplc following reduction/solubilization and reoxidation. the protein isolated was identified as met-(-1) angio ...19883071185
incidence and biotyping of aeromonas species from the environment.a survey was carried out to establish the incidence rate of aeromonas species in various environmental samples with a view to ascertaining their reservoirs and distinguishing the biotypes. high rates of isolation were obtained from snails and water. this indicates that these samples are likely reservoirs and therefore constitute high sources of human infection. low incidence rates were realised from poultry, cattle and humans, thus suggesting that these samples may be transient hosts. two specie ...19883071728
enzymatic characterization of three aeromonas species using api peptidase, api "osidase," and api esterase test kits.an enzymatic characterization of 16 strains of aeromonas species including a. hydrophila (7), a. sobria (5), and a. caviae (4) was carried out using api peptidase (strips numbered 1, 2, 3, 4, 5, and 6); api esterase and api "osidase" test strips. a total of 89 substrates was used in the assay and included 59 arylamides (aminopeptides), 10 esters, and 20 carbohydrates. all three species were remarkably uniform in their reactivities. nineteen (32%) of the arylamide substrates used were hydrolyzed ...19883072150
aeromonas hydrophila-punctata in two sicilian reservoirs: density of the organisms in relation to physico-chemical and bacteriological parameters. 19883072527
vibrios and aeromonas.there are many similarities in the vibrionaceae that cause human illness in the united states (see table 1). vibrios are characteristically indigenous to marine, estuarine, and brackish environments. they are distributed mainly in gulf of mexico coastal water, and these organisms "bloom" when the water is warm. outbreaks of disease in humans frequently occur in summer, coinciding with multiplication of vibrios in warm water. sporadic cases and small outbreaks of cholera continue to occur in pers ...19883074121
a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...19883076719
epidemiology of aeromonas and plesiomonas diarrhoea. 19883077628
comparison of methods for the induction, propagation and recovery of l-phase variants of aeromonas spp. 19883077629
aeromonas and plesiomonas in the environment: value of differential biotyping of aeromonads. 19883077630
human extraintestinal infections caused by aeromonas species. 19883077631
drug-resistant plasmids from fish pathogens.the epidemiological surveillance of drug-resistant strains of the fish pathogenic bacteria vibrio anguillarum and pasteurella piscicida carrying transferable r plasmids in fish farms is described. the dna structure of r plasmids, and the drug-resistant determinants of r plasmids from the fish pathogens aeromonas hydrophila,aeromonas salmonicida, edwardsiella tarda, v. anguillarum, and p. piscicida are discussed.19883079184
fatal infection with aeromonas sobria and plesiomonas shigelloides. 19863081156
[transmission of vibrio cholerae. possible role of the camel].choleraic vibrio uses the moving means that man discovers to his own use. camels (passive and active vehicles of large quantity of crude water) were experimentally infected with a vibrio (tox. +) and an aeromonas (tox. +). suckling young camels eliminate them for 6 days and grown-up camels for 25 days. this experience, associated with the discovery of vibrio non 01 and shigella in the caravan camel dungs, lead them to become a potential vector of spreading from one oasis to another.19863096512
melanogenesis in murine b16 cells exposed to aeromonas hydrophila cytotoxic enterotoxin.specific markers (growth, melanogenesis) of b16 murine melanoma cells in culture were used as indicators of toxin production by aeromonas hydrophila. cytotonic enterotoxinlike activity (inhibited growth, raised tyrosinase activity, and melanin accumulation) occurred at cytotoxic end points of purified beta-hemolysin and several culture filtrates. antihemolysin rabbit serum inhibited this activity. a hemolysin-neutralized culture filtrate concentrate (10x) failed to elevate tyrosinase relative to ...19863098398
characterization of extracellular metallo- and serine-proteases of aeromonas hydrophila strain b51.the extracellular proteases of aeromonas hydrophila b51 were stable on heating (56 degrees c) and on storage at 4 degrees c or -20 degrees c. inhibitor studies showed that 72% of the total activity was inhibited by edta (a metalloprotease inhibitor) and 26% was inhibited by phenylmethanesulphonyl fluoride (a serine protease inhibitor). analytical isoelectric focussing revealed the presence of 33 proteins in the crude extracellular products. using a casein overlay technique three separate zones o ...19863098904
purification of cytotoxic enterotoxin of aeromonas sobria by use of monoclonal antibodies.cytotoxic enterotoxin of aeromonas sobria was purified by affinity chromatography with monoclonal antibodies. the purified enterotoxin gave a single protein band in polyacrylamide gradient gel electrophoresis and its mol. wt estimated by this technique was 63,000; it had a pi of 6.2. the purified enterotoxin caused fluid accumulation in rat ileal loops and in infant mice, was cytotoxic to cultured cells, was haemolytic to human erythrocytes, and was lethal to mice after intravenous injection. th ...19873104592
medical leeches as sources of wound infection. 19873107664
in vitro activity of ro 23-6240 (am-833): a new fluoroquinolone.the in vitro activities of ro 23-6240 (am-833) and four comparative fluoroquinolones were studied. minimal inhibitory concentrations (mics) of ro 23-6240, which inhibited at least 90% of strains, were less than or equal to 0.03-1 microgram/ml for staphylococcus aureus, staphylococcus epidermidis, enterobacteriaceae, acinetobacter anitratus aeromonas hydrophila, branhamella catarrhalis, and haemophilus influenzae, 2-8 micrograms/ml for staphylococcus saprophyticus, streptococci, diphtheroids, and ...19873107872
enumeration of pathogenic bacteria from sewage sludge in kuwait.domestic sewage in kuwait is mainly treated by an activated sludge process. sewage sludges at different treatment stages and the digested sludge were studied for their content of pathogenic bacteria. two gram-positive cocci and eight gram-negative bacilli were isolated and enumerated in the sewage sludges and in raw sewage. during march, aeromonas hydrophila was the dominant bacterium isolated. serratia liquefaciens in raw sewage and sludge-2, and salmonella species in digested sludge were domin ...19873121985
critical ionizing groups in aeromonas neutral protease.aeromonas neutral protease possesses two residues critical to its activity. one has a pka of 5.5 in both the free enzyme and the enzyme-substrate complex and must be deprotonated for maximal activity. the other, which ionizes at ph 7.1 in the free enzyme and at ph 7.4 in the enzyme-substrate complex, must be protonated for optimal enzyme action. the protease is reversibly inhibited by aminoacyl hydroxamates, peptides containing a phenylalanyl residue, phosphoryl-l-phenylalanylglycylglycine, and ...19883123480
effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens.in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ...19883127604
catabolism of arginine, citrulline and ornithine by pseudomonas and related bacteria.the distribution of the arginine succinyltransferase pathway was examined in representative strains of pseudomonas and related bacteria able to use arginine as the sole carbon and nitrogen source for growth. the arginine succinyltransferase pathway was induced in arginine-grown cells. the accumulation of succinylornithine following in vivo inhibition of succinylornithine transaminase activity by aminooxyacetic acid showed that this pathway is responsible for the dissimilation of the carbon skele ...19873129535
pathogenicity of aeromonas sp. for balb/c mice. 19883130510
purification and characterization of salmolysin, an extracellular hemolytic toxin from aeromonas salmonicida.an extracellular hemolytic toxin of aeromonas salmonicida, termed salmolysin, was purified 945-fold by ammonium sulfate precipitation, anion-exchange chromatography on deae-cellulose, and gel filtration chromatography on sephadex g-100 and sepharose 2b. salmolysin appeared homogeneous upon cellulose acetate membrane electrophoresis and immunodiffusion analysis. the molecular weight of the toxin was estimated to be approximately 200,000 by the sedimentation equilibrium method. the uv absorption s ...19883136147
wound infection with aerogenic aeromonas strains: a review of twenty-seven cases.aeromonas spp. have been implicated in a wide spectrum of human disease including wound infection. a review is made of local experience of 27 wound infections from which aerogenic strains of aeromonas were recovered; of these, aeromonas was isolated in pure culture from five infections. twenty-five infections were related to trauma and were community acquired, while two were probably nosocomial. of the 25 community acquired infections, 16 were associated with contamination by soil and one with w ...19883137035
[development of aeromonas hydrophila septicemia in a young healthy woman]. 19883138333
acute diarrhea in children in shanghai. 19883138335
aeromonas schubertii, a new mannitol-negative species found in human clinical specimens.in 1983 the vernacular name enteric group 501 was coined for a group of strains that had been referred to our laboratory as "possible vibrio damsela that does not require nacl for growth." by dna-dna hybridization (hydroxyapatite method, 32p, 60 and 75 degrees c), six strains of enteric group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees c and 71 to 93% at 75 degrees c; 0 to 1% divergence). type strains of all aeromonas species and reference strains of six other ...19883139706
[disease of the fins of rhamdia sapo. isolation of the etiological agents and experimental infection].during intensive culture of south-american catfish, rhamdia sapo fingerlings an epizootic similar to fin rot was developed. aeromonas hydrophila and pseudomonas aeruginosa were isolated and they were treated and controlled with daily short dip, 1 ppm furanace. the disease was experimentally reproduced and we concluded that it is result of interaction of unfavorable environment conditions such as: low dissolved oxygen, increase of solids in suspension; host condition and the presence of aeromonas ...19883140294
aeromonas species in a municipal water supply of a central european city: biotyping of strains and detection of toxins.in the course of a one-year study, 78 out of 239 samples from a central european municipal water supply were found to contain aeromonas species. biotyping and hemolysin titers according to the criteria of burke et al. (1) showed that almost 50% of the tested strains can be presumed to be enterotoxin-producing. the remaining microbiological parameters met international standards (20).19883140537
evaluation of 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) in broth and agar media for identification of pseudomonas aeruginosa.brain heart infusion broth and mueller-hinton agar containing the compound 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) were evaluated as selective media for identifying pseudomonas aeruginosa. of the 192 pseudomonas spp. and 68 additional oxidase-positive or glucose-nonfermenting gram-negative organisms tested, only p. aeruginosa (120 of 121 isolates) and certain strains of pseudomonas cepacia, aeromonas hydrophila, and achromobacter xylosoxidans wer ...19883141473
experimental evidence for enteropathogenicity in aeromonas veronii.eleven ornithine-positive strains of aeromonas (9 a. veronii and 2 provisionally classified as aeromonas species ornithine positive) were tested for ability to cause fluid accumulation in the rabbit ileal loop. all eight beta-hemolytic strains caused fluid accumulation. gel diffusion analysis revealed that the a. veronii beta-hemolysin was serologically related to the a. hydrophila beta-hemolysin, a known enterotoxic molecule. the biological activity of the a. veronii hemolysin was neutralized b ...19883143475
comparative in vitro activity of the new difluoro-quinolone temafloxacin (a-62254) against bacterial isolates from cancer patients.the in vitro activity of temafloxacin, a new difluoro quinolone agent, against 725 bacterial isolates representing 32 species was evaluated in comparison with that of ciprofloxacin. temafloxacin inhibited the majority of enterobacteriaceae isolates at a concentration of less than or equal to 0.5 microgram/ml. it was also extremely active against acinetobacter spp. and aeromonas hydrophila. its activity was 2-8 fold less than that of ciprofloxacin against most gram-negative isolates. methicillin- ...19883143585
bacterial hemolysins as virulence factors. 19883144241
[evaluation of 2 culture media for the isolation and enumeration of motile aeromonas in different kinds of water].in recent years an increasing incidence of aeromonas-related cutaneous infections and gastroenteritis has raised a serious public health problem. it appeared therefore timely to define a specific method allowing the rapid isolation and enumeration of the bacteria in their various aquatic habitats. in this line of research we have compared the growth of aeromonas originating from different aquatic sources and raised on two media, i.e. rs-agar and pxa-agar. whatever the aquatic system we observed ...19883144424
properties of aeromonads and their occurrence and hygienic significance in drinking water.in the netherlands, aeromonads in drinking water have attracted much attention in recent years. this development was caused by a sudden increase of the aeromonas density in the drinking water of the municipal dune waterworks of the hague and reports about the possible health significance of these organisms in drinking water. literature data indicate that representatives of the motile aeromonas species a. hydrophila, a. sobria and a. caviae generally have been observed in larger percentages of di ...19883146172
aeromonas hydrophila infection after cockles ingestion. 19883148523
in vitro activity of fleroxacin (ro23-6240), a new fluorinated 4-quinolone against isolates from cancer patients.the in vitro activity of fleroxacin (ro23-6240; am 833), a new fluorinated 4-quinolone, was compared to that of ciprofloxacin, enoxacin and a-56620, against 747 isolates from cancer patients. fleroxacin inhibited more than 90% of enterobacteriacea isolates at a concentration of less than or equal to 0.25 micrograms/ml. it was also extremely active against aeromonas hydrophila and haemophilus influenzae isolates with mic90 values of 0.12 and 0.06 micrograms/ml, respectively. the mic90 for acineto ...19883149568
are medicinal leeches an infection hazard? 19873153606
[aquatic contamination of wounds by aeromonas sobria after bathing in the river]. 19853160041
in vitro activity of lomefloxacin (sc-47111; ny-198), a difluoroquinolone 3-carboxylic acid, compared with those of other quinolones.lomefloxacin (sc-47111; ny-198) is a new difluoroquinolone agent. it inhibited 90% of escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, morganella morganii, proteus vulgaris, serratia marcescens, salmonella spp., shigella spp., aeromonas spp., yersinia spp., haemophilus influenzae, and neisseria gonorrhoeae at less than or equal to 2 micrograms/ml. lomefloxacin inhibited 90% of pseudomonas aeruginosa at 4 micrograms/ml. lomefloxacin was equal in activity ...19883164987
aeromonas hydrophilia infection in burn patients.aeromonas hydrophilia rarely infects burn wounds. three cases of early a. hydrophilia burn wound infection, with one death, are reported. a history of extinguishing the fire with dirty water or by rolling in dirt should alert the physician to consider a. hydrophilia as a possible infection organism.19883167587
suppurative thrombophlebitis due to aeromonas.a patient developed lethal suppurative thrombophlebitis and adjacent soft-tissue infection caused by aeromonas. potential risk factors included corticosteroid therapy and the use of warm tap water compresses at the site of intravenous catheter-related phlebitis. this case demonstrates the rapidly invasive characteristics of aeromonas and the need for early surgical intervention in suppurative thrombophlebitis.19883168427
dna:dna reassociation analysis of aeromonas salmonicida.dna from 26 aeromonas salmonicida strains, namely 11 'typical' and 15 so-called 'atypical' strains, was used to assess the taxonomic relatedness within the species. the genomes were characterized by determination of dna base composition, dna:dna reassociation, calculation of sequence divergence following reassociation, and by genome size estimations. by comparison with dna obtained from controls and the aeromonas hydrophila group, a. salmonicida strains were determined to be correctly placed wit ...19883171541
[the occurrence of aeromonas hydrophila in birds]. 19883176762
isolation and characterization of motile aeromonas from human, food and environmental specimens.from july 1985 to march 1987, the occurrence of motile aeromonas sp. in stool, food and environmental specimens was investigated to assess their pathogenic significance and to determine sources and routes of infection. a total of 9366 stool specimens were examined; aeromonas was isolated from 11.1% of diarrhoeal stools and 2.2% of normal stools (p less than 0.001). aeromonas counts in food specimens, which included minced beef, pork and chicken, seafood and various vegetables and their products, ...19883181307
the in-vitro activity of pd127,391, a new quinolone.mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...19883182416
the comparative in-vitro activity of ofloxacin.the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...19883182468
comparison of blood agar, ampicillin blood agar, macconkey-ampicillin-tween agar, and modified cefsulodin-irgasan-novobiocin agar for isolation of aeromonas spp. from stool specimens.the performance of four media for the isolation of aeromonas strains from stool specimens, the importance of ampicillin-susceptible aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for aeromonas strains were evaluated in two phases. in the first phase, 36 of 1,672 stool specimens yielded aeromonas isolates. ninety-seven percent of the isolates were detected on blood agar containing 20 micrograms of ampicillin per ml (ab ...19883183020
[aeromonas hydrophila dacryocystitis. case report].the present paper reports on a case of severe dacryocystitis with a dacryolith, caused by aeromonas hydrophila. on the basis of this case the authors show that a therapy effective in vitro does not lead to elimination of bacteria in all cases, so that further therapeutic considerations are necessary.19883184754
aeromonas-associated diarrhea in children.in a 27-month prospective study, aeromonas spp. were isolated from 7.3% of children with diarrhea and from 2.2% of controls. in 32 patients with diarrhea, ranging in age from 1 to 27 months old, aeromonas spp. were the only potential bacterial enteropathogens isolated. principal symptoms of aeromonas-associated diarrhea were vomiting, fever and bloody stools. diarrhea was often self-limiting and lasted for 10 days or less in 90% of patients. no secondary spread of diarrhea among close contacts w ...19883186340
biochemical and toxigenic characteristics of aeromonas spp. isolated from diseased mammals, moribund and healthy fish.in this study we describe biochemical, toxigenic and surface characteristics of 33 motile aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. aeromonas hydrophila, a. caviae and a. sobria were isolated from both mammals and fish but at a different incidence. aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, catt ...19883188376
the humoral immune response of rainbow trout (salmo gairdneri) immunised by various regimes and preparations of aeromonas salmonicida antigens.antibody production in rainbow trout to extracellular antigens was investigated. the following antigen preparations and immunisation regimes were used: native extracellular products (ecp) in freund's complete adjuvant (fca), intraperitoneally (i.p.) with and without booster; formalinized ecp in fca, i.p. with and without booster; washed, formalinized a. salmonicida cells in fca, i.p., with booster; native ecp in saline, i.m., four weekly injections at two different doses, 45 micrograms and 6 mic ...19883188377
in vitro activity of u-76,252 (cs-807), a new oral cephalosporin.u-76,252 is the prodrug of u-76,253. mics of u-76,253 were 0.015 to 0.06 microgram/ml for greater than or equal to 90% of the strains of streptococcus spp., haemophilus influenzae, and proteus mirabilis; 0.25 to 1 microgram/ml for branhamella catarrhalis, escherichia coli, klebsiella spp., and citrobacter diversus; 1 to 8 micrograms/ml for staphylococcus spp.; and 2 to greater than 16 micrograms/ml for other members of the family enterobacteriaceae and aeromonas hydrophila; for 72% of the latter ...19883190194
selection for virulence in the fish pathogen aeromonas salmonicida, using coomassie brilliant blue agar.coomassie brilliant blue agar was used to quantify the frequency of the a-layer phenotype in different isolates of aeromonas salmonicida. hydrophilic, non-clumping isolates of a. salmonicida consisted predominantly of the a-layer minus phenotype. these bacteria were avirulent by intraperitoneal injection into susceptible brook trout (salvelinus fontinalis) and could not be reisolated from infected fish. by contrast, hydrophobic, clumping isolates were predominantly of the a-layer positive phenot ...19883193563
inhibitory activity of antibiotic-producing marine bacteria against fish pathogens.the activity of antibiotic-producing marine bacteria was assayed against bacterial fish pathogens belonging to the genera vibrio, aeromonas, pasteurella, edwardsiella, yersinia and pseudomonas with the aim of evaluating the possible use of these marine strains for controlling epizootics in aquaculture. inhibition tests on solid medium showed that, in general, the majority of fish bacteria were strongly sensitive to the marine bacteria. only two strains (edwardsiella tarda and pseudomonas aerugin ...19883204073
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