the efficiency of aquocobalamine as an antidote in cyanide poisoning when given alone or combined with sodium thiosulfate.the antidotal activities of aquocobalamineacetates and sodium thiosulfate were tested in guinea pigs and cats. the animals were attached to artificial respirators throughout the experiment and were poisoned with a continuous infusion of sodium cyanide solution (4.1 mumol/kg.min nacn). the rate of action of each antidote was determined from the time taken for the hcn exhalation to drop below the level of 100 nmol/kg.min in quinea pigs, and to values below 25 nmol/kg.min in cats; the detoxifying c ...19751242295
new assay for pregnenolone and progesterone 17alpha- hydroxylase based on the specific substitution of a tritium situated on carbon 17.a new assay for the measurement of steroid 17alpha-hydroxylase activity in beef adrenals is described. this method is based on the biochemical mechanism of the enzymic reaction, i.e. the direct and stereospecific substitution of the proton located on the hydroxylated position. progesterone or pregnenolone specifically labelled on the 17 position are solubilized in the incubation mixture with the help of tween 80 and incubated under optimal conditions. the tritium enzymically released from the su ...19761248468
separation of two hepatitis b human antibody fractions by cold-ethanol precipitation.antibodies to three different samples of hepatitis b antigen-positive serum were found to be absent from a cohn fraction ii hepatitis b immune globulin preparation, but present in the whole plasma from which the immune globulin was made. fractions containing the antibodies specific for the three antigen samples were isolated from the plasma by euglobulin precipitation, and the antibodies were characterized as to immunoglobulin class and precipitability in the cohn fractionation procedure. they a ...19761251569
familial clustering of selective iga deficiency.serum iga levels of 35 healthy iga-deficient index cases, of their 180 first-degree relatives, and of 31 other family members were studied. iga deficiency was detected in 7.2% of the first-degree relatives, which is significantly more than the 0.25% frequency of iga deficiency in healthy finnish blood donors. subnormal serum iga levels were found in additional 13 (7.2%) first-degree relatives. the familial clustering of iga deficiency seemed to be controlled by multigenic factors. iga-deficient ...19761251582
[diagnostic problems in gout in a 44-year-old man]. 19761251615
the effect of subdermal testosterone filled polydimethylsiloxane implants on spermatogenesis in rhesus monkeys (macaca mulata).previously it was reported that testosterone-filled polydimethyl silicone (pds) implants induced azoospermia in male rabbits without chronically altering plasma testosterone concentration or the weight or secretory activity of accessory sex glands. assayable serum luteinizing hormone (lh) and testicular testosterone secretion were completely suppressed. testosterone emanating from the pds capsule maintained libido but failed to support spermatogeneis in the rabbits. to test results in subhuman ...19761261261
the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens.counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ...19761262695
attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization.four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ...19761262709
yersinia pestis infection in vietnam. ii. quantiative blood cultures and detection of endotoxin in the cerebrospinal fluid of patients with meningitis.quantitative blood cultures were obtained from 42 patients with acute yersinia pestis infection to determine whether the concentration of bacteria in blood influenced the clinical severity and outcome of illness. in 17 bacteremic patients, colony counts in blood cultures ranged from less than 10 to 4 x 10(7)/ml. three of five patients with colony counts of greater than 10(2)/ml died, and two patients survived episodes of hypotension. results from plasma limulus tests were positive at the time of ...19761262715
[use of erythrocyte diagnostic agents for the determination of integrative association constants].the authors described a method of determination of integrative association constant differing from intrinsic association constant by the value of the valency of macromolecular antigen. the method is based on the using of erythrocytes on whose surface one of the components is fixed, whereas the other is in free state. application of equations was demonstratedon a number of examples with determination of integrative association constant. the data obtained permitted to assess qualitative peculiarit ...19761266452
[cryosurgery of the esophagus and the cardia in animal experiments]. 19761272986
the toxic effects of dieldrin in rats: a reevalution of data obtained in a two-year feeding study. 19761273845
the isolation and identification of noradrenaline and dopamine from the venom of the honey bee, apis mellifica. 19761273857
[changes in the activity of succinate dehydrogenase in the cells of different brain structures under the effect of uhf field of low intensity]. 19761274298
glial cells and extracellular potassium: their relationship in mammalian cortex.simultaneous recordings were made of glial cell potentials and the extracellular potassium concentration ([k+]o) in cat cortex in an attempt to provide more quantitative information about the sensitivity of mammalian neuroglia to changes in [k+]o. a penicillin epileptogenic focus served to generate both transient and sustained elevations in [k+]o, thus allowing measurement of glial membrane potential (vm) at both resting and increased [k+]o levels many times during the same experiment. resting v ...19761276917
development of a competitive radioimmunoassay and a sandwich enzyme-linked immunosorbent assay for recombinant human granulocyte colony-stimulating factor. application to a pharmacokinetic study in rats.a competitive radioimmunoassay (ria) and a sandwich enzyme-linked immunosorbent assay (elisa) for the measurement of recombinant human granulocyte colony-stimulating factor (rhg-csf) in rat serum was developed using polyclonal antibody obtained by immunizing rabbits with rhg-csf. the anti-rhg-csf antibody did not recognize other colony-stimulating factors or interleukins. ria and elisa gave satisfactory reproducibility as judged by intra- and inter-assay precision. good agreements between the ri ...19921280299
acrylamide-induced alterations in axonal transport. biochemical and autoradiographic studies.alterations in the axonal transport of proteins, glycoproteins, and gangliosides in sensory neurons of the sciatic nerve were examined in adult male rats exposed to acrylamide (40 mg ip/kg body wt/d for nine consecutive days). twenty-four hours after the last dose, the l5 dorsal root ganglion (drg) was injected with either [35s]methionine to label proteins or [3h]glucosamine to label glycoproteins and gangliosides. the downflow patterns of radioactivity for [35s]methionine-labeled proteins and [ ...19921282332
regeneration of an adult peripheral nerve preparation in culture.the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process.19921282333
antigen f1 from yersinia pestis forms aqueous channels in lipid bilayer membranes.antigen f1 is a protein of 17 kda produced by yersinia pestis when it is cultured at 37 degrees c. when incorporated into planar lipid bilayer membranes this protein induces fluctuations on membrane conductance typical of the formation of ionic channels. these fluctuations reveal two distinct unitary conductance sizes, one in the range of 800 to 1400 ps and the other in the range of 140 to 600 ps. zero current potential measurements in the presence of a salt gradient show that the channel is not ...19921284806
child health surveillance. surveillance of the child under 5.1. general health checks should be made at the following times as outlined in these guidelines: initial neonatal assessment 7-10 day check, 6-week check, 7-9 months: general examination with particular attention to hearing and vision, 18-24 months: check with special attention to gait, speech and understanding, 36-42 months: general examination and developmental assessment, 2. parental concern over a child's special senses should be carefully followed up and investigated. 3. immunization schedul ...19921285363
orientation bandwidth: the effect of spatial and temporal frequency.the orientation bandwidths of psychophysically defined channels of human vision were estimated by two techniques for a wide range of spatial and temporal frequencies. the first technique was an adaptation paradigm, where the subjects' ability to see patterns of various orientations was measured before and after adapting to a high contrast pattern. the second technique evaluated subjects' ability to discriminate between two gratings of different orientations in relation to their ability to detect ...19921287993
[biological characteristics of tumor growth delay in human nasopharyngeal carcinoma xenograft (npc-837) after irradiation].tumor growth and cell cycle of human nasopharyngeal carcinoma xenograft in nude mice (npc-837) after irradiation were studied. after cobalt-60 irradiation of 5.0gy, 7.5gy, 10gy, 15gy and 20gy, the tumor volume was reduced to 80, 70, 50, 35 and 10 percent of the control, respectively. however, the tumors began to grow again. the time (in days) the tumors took to reach the volume of the control was radiation dose-dependent. with 20 gy irradiation the tumor volume was kept at 10% of the control lev ...19921291288
[dna probe for detecting yersinia pestis and serovariant i of yersinia pseudotuberculosis by detecting specific dna repeating sequences].in order to construct a dna probe for the plague pathogen detection, we have obtained the recombinant plasmid prd100 carrying an ecori-flanked 140 bp fragment from the genetically silent region of yersinia pestis species-specific plasmid pyp1. when used as a dna probe for hybridization of dna from various strains of 25 bacterial species, this dna fragment was shown to have the complementary sequences in all investigated yersinia pestis strains (200), including the plasmid pyp1 lacking ones, and ...19921298882
[serovariation of the plague pathogen capsular antigen].the phenomenon of serovariation in the capsule antigen of the plague pathogen has been found. the synthesis of fi-serovar is determined by the expression of the caf1m gene. the damages in the caf1m gene structure result in production of the fi1 and fi2 serotypes. expression of the caf1m gene does not affect the secretion of fi-antigen.19921298883
[studies of the lymphatic system in mice infected with trichinella pseudospiralis as well as infected and stimulated by phytohaemagglutin (pha-p)].mice cfw were infected per os with trichinella pseudospiralis (150 larvae/mice). the mice were divided in two groups: nonstimulated and stimulated intraperitoneally with pha-p at dose 10 mg/kg body weight. the animals were killed 7, 14, 21, 28, 42, 56, 70 and 150 day post infection (d.p.i.). spleen et mesenteric lymph nodes were examined histopathologically (hematoxylin-eosin) and histochemically (nonspecific esterase reaction) for differentiation of t lymphocytes. the obtained results reveal th ...19921299055
[effect of subinhibitory concentrations of antibiotics on catalase activity of plague microbes].it was shown that the presence of subinhibitory concentrations of ampicillin, cefotaxime or gentamicin in the cultivation medium had a marked inhibitory effect on the catalase activity of plague microbe. the effect depended on the characteristic features of plague microbe strains and the incubation temperature. when the cells of a virulent strain of the plague microbe y. pestis 1300 were cultivated at a temperature of 37 degrees c on a medium containing the subinhibitory concentrations of ampici ...19921300927
[antibiotic sensitivity of plague microbe strains from foreign countries].the method of serial dilutions on the hottinger agar was applied to comparative assay of antibiotic sensitivity in 50 strains of the plague microbe isolated abroad and in 5 strains isolated in the plague focus in the central caucasus. the antibiotics used in the assay were the following: streptomycin, gentamicin, doxycycline, monomycin, kanamycin, tetracycline, erythromycin, ristomycin, lincomycin and polymyxin m. irrespective of the origin, all the isolates were resistant to erythromycin, linco ...19921300928
[doxycycline in the prevention of experimental plague induced by plague microbe variants].a comparative study was performed on the efficacy of doxycycline in experimental plague infection induced in albino mice by strain 231 of the plague microbe and its variant 231 fra- deprived of the ability to produce the fraction i antigen. it was shown that the ld50 for strain 231 during animal treatment with doxycycline was significantly higher than that for variant 231 fra-. prophylaxis of the plague infection caused by the fra- forms of the plague microbe required significantly higher doses ...19921300930
[localization of the dna segment coding for the synthesis of fraction i on the pyt plague pathogen plasmid].fragmentation of the pyt plasmid of the plague pathogen by ten restriction endonucleases has been studied. the evidence has been obtained in support of the possible presence of the movable genetic element containing a hindiii site within the plasmid pyt. the gene encoding the i fraction of the plague pathogen has been cloned. the physical map of the pyt plasmid has been constructed with the use of restriction endonucleases bamhi, xhoi, bsteii, smai, ecori, and hindiii. the fragment of the plasmi ...19921301497
[a comparative cytofluorimetric analysis of the blood leukocytes in guinea pigs exposed to the phospholipase d and antigens of the causative agent of plague].the influence of y. pestis phospholipase d on the physiological state of leukocytes in the blood of guinea pigs was studied in vivo by flow impulse fluorometry with the use of fluorochrome acridine orange. during the first hours of observation the intensity of leukocyte fluorescence increased due to a rise in the number of polymorphonuclear leukocytes and changes in the permeability of cell membranes. further changes in the intensity of the fluorescence of the material under study after 24 hours ...19921301664
[the sensitivity of bacterial strains of yersinia pestis isolated from voles to normal human serum].the work deals with the results of the study of the viability of y. pestis strains, isolated from voles in different natural foci of plague, in human normal serum (hns) and its dependence on the assimilation of ions of exogenic iron. the cultures isolated at the transcaucasian mountain focus of infection and having no small plasmid pyp were found highly sensitive to hns. the introduction of the sources of iron, such as hemoglobin or ferritin, into the serum decreased its bactericidal effect.19921301669
[the action on brain functions in white rats of the immunostimulant freund's complete adjuvant].the effect of immunostimulator--freund's adjuvant complete on the nociception and learning of white rats using food-obtaining and avoidance of electric shock techniques has been studied. it has been shown that the adjuvant in doses for active immunization causes itself the expressed changes in animal behaviour. the adjuvant's injections significantly increases the learning ability of animals both with negative and positive reinforcement as compared with control one. the changes in pain severity ...19921301805
expression and release of neuroregulators during development: monoamines and neuropeptides.the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...19921306802
[rheumatic manifestations of acquired immunodeficiency syndrome (aids)].120 aids patients (mean age 33 +/- 9 years, 108 males) were evaluated regarding rheumatic manifestations. according to cdc's classification, 18.3% belonged to group ii, 28.3% to group iii, and 53.4% to group iv. arthralgia was present in 33 patients (27.5%), and in only 8 could be associated with infections other than hiv (5 cases of tuberculosis, 3 p. carinii, and 1 gonococcal infection). incidence of arthralgia was equal in either sex. arthritis was present in 8 patients, 2 of them with reiter ...19921307073
evaluation of three serological tests for the detection of human plague in northeast brazil.the passive haemagglutination (pha) test, enzyme-linked immunosorbent assay (elisa) and the dot enzyme-immunosorbent assay (dot-elisa) were used to detect the levels of igg antibodies against the fraction 1 (f1) antigen of yersinia pestis in sera of plague-infected patients from northeast brazil. twenty three selected pha-positive sera of subjects with bacteriological confirmation of plague were also positive in the dot-elisa but only 19 were detected by the conventional elisa technique. another ...19921308559
[sodoku--a case report].rat-bite fever results from an infection with the organism spirillum minus. the authors report a symptomatic patient with fever, malaise, and ulcer in forehead after a rodent exposure. the organism was identified in darkfield examination of the ulcer exudate. penicillin was the drug used with clinical improvement of symptoms.19921308939
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...19921312014
regulation of interleukin-1 receptors on att-20 mouse pituitary tumour study the cellular mechanisms of interleukin-1 (il-1) in the pituitary corticotroph, we studied the properties of il-1 receptors on a mouse pituitary acth-producing cell line, att-20. scatchard plot analysis revealed a single type of receptor with a kd (dissociation constant) of 93 pm, and 482 binding sites/cell. [125i]il-1 alpha binding competed with il-1 alpha and il-1 beta in an equimolar fashion. a 24 h pre-incubation with either crh, epinephrine or nor-epinephrine increased the [125i]il- ...19921312014
in situ hybridization of calcium/calmodulin dependent protein kinase ii and tau mrnas; species differences and relative preservation in alzheimer's disease.abnormal phosphorylation of the microtubule associated protein tau component of neurofibrillary tangles (nfts) in alzheimer's disease (ad) may result from alterations in protein kinase expression. calcium/calmodulin dependent protein kinase ii (cam kinase ii) has been shown to phosphorylate tau in vitro in such a way to decrease its electrophoretic mobility. a68, apparently a modified form of tau in ad brain, also shows abnormal phosphorylation and slower mobility than tau. to further examine th ...19921312209
survival of hepatitis a virus on human hands and its transfer on contact with animate and inanimate surfaces.the survival of hepatitis a virus (hav; strain hm175) on the hands of five volunteers was determined by depositing 10 microliters of fecally suspended virus on each fingerpad and eluting the inoculum after 0, 20, 60, 120, 180, and 240 min. the amount of virus recovered from each fingerpad at 0 min was approximately 6.0 x 10(4) pfu. at the end of 4 h, 16 to 30% of the initially recoverable virus remained detectable on the fingerpads. hav inocula (10 microliters; approximately 1.0 x 10(4) pfu) pla ...19921315331
leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa.human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ...19921316878
[leu31, pro34] npy, a selective functional postjunctional agonist at neuropeptide-y receptors in anaesthetised rats.neuropeptide y (npy), a sympathetic cotransmitter, has both prejunctional and postjunctional actions in the cardiovascular system. in the bioassay system used here npy attenuates cardiac vagal action (an indicator of prejunctional or y2 action) and increases blood pressure (an indicator of postjunctional or y1 action). [leu31,pro34]npy has little or no prejunctional activity i.e. does not attenuate cardiac vagal action, but increases blood pressure as effectively as npy. [leu31,pro34]npy can the ...19921317023
kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release.the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ...19921317026
different preparations of zymosan induce glycogenolysis independently in the perfused rat liver. involvement of mannose receptors, peptide-leukotrienes and prostaglandins.zymosan (non-boiled) induced glycogenolysis biphasically, with no lag time, in the perfused rat liver. after the zymosan was boiled, it could be separated into two fractions, both of which stimulated glycogenolysis independently. the soluble fraction of boiled zymosan (zymosan sup) showed homologous desensitization, indicating that zymosan sup-induced glycogenolysis is a receptor-mediated event. mannan (polymannose), which is known to be a biologically active component of zymosan, induced a glyc ...19921317164
radiation-induced generation of chlorine derivatives in n2o-saturated phosphate buffered saline: toxic effects on escherichia coli cells.the radiolysis of aqueous chloride solutions has been investigated using pulse and steady-state methods. we have found a correlation between the yields of cl2- and hocl formed in pulse-irradiated n2o-saturated solutions. the yields increased with the increasing concentrations of cl- and phosphate. phosphate enhanced the yield of cl2- in neutral solutions because of a proton transfer from h2po4- to hocl- with a rate constant of (2.6 +/- 0.5) x 10(8) m-1s-1. hocl could not be detected in pulse-irr ...19921317323
the occurrence of periodontitis-related microorganisms in relation to titanium implants.the peri-implant space was examined for the presence of periodontitis-related microorganisms in 19 patients. all patients had been fitted with osseointegrated titanium implants which were clinically sound at the time of examination and all had been prescribed daily 0.2% chlorhexidine mouthrinse. in 17 patients, two contralateral fixtures in patients were selected, the remaining 2 patients had only one fixture, and associated mucosal clinical variables assessed for plaque accumulation, gingival i ...19921317427
alpha 1-adrenergic effects on intracellular ph and calcium and on myofilaments in single rat cardiac cells.1. the cellular effects of alpha 1-adrenoceptor stimulation by phenylephrine were studied in the presence of propranolol in single cells isolated from the ventricles of rat hearts. 2. phenylephrine (10-100 microm) induced a biphasic pattern of inotropism in these cells: a transient negative followed by a sustained positive inotropic effect as usually observed in cardiac tissues. 3. in snarf-1-loaded cells, phenylephrine induced an alkalinization. this effect was reversible on wash-out and inhibi ...19921317431
regulation of pregnenolone synthesis in c6-2b glioma cells by 4' experimental model to study synthesis of cholesterol and pregnenolone from the precursor mevalonolactone (mva) was developed in c6-2b glioma cells. the steroidogenic capability of this cell line and the regulation of pregnenolone production by 4'-chlorodiazepam (4'cd), a specific ligand for the mitochondrial diazepam binding inhibitor (dbi) receptor (mdr), were investigated. cells maintained in serum-free media were incubated with lovastatin (20 microm) and two inhibitors of pregnenolone meta ...19921317582
plasma testosterone concentration in adult male rats following acquisition of copulation-illness associations.prior research has identified stimuli and procedures that elicit increments in plasma testosterone in copulating male rats. in the present experiment, we demonstrate that associative inhibition of copulatory behaviors in male rats is not correlated with and cannot be attributed to a conditioned suppression of testosterone. each male rat was paired with an inaccessible estrous female for 7 min and was then given an opportunity to copulate. two groups received an injection of either lithium chlori ...19921317591
the clinical significance of in vitro cytomegalovirus susceptibility to antiviral drugs. 19921317603
[camp-binding protein--a protein kinase of the plague pathogen].in y. pestis a cyclic amp-binding protein was detected, isolated to a homogeneous state, and its physico-chemical properties were studied. the protein is a highly molecular compound with a molecular weight of 180 kd, capable of being released into the environment in the process of cell growth and having protein kinase activity, not depending on the presence of cyclic amp. y. pestis neuraminidase is one of the substrates appearing due to the action of protein kinase detected in this study. y. pes ...19921324549
epstein-barr virus in acquired immune deficiency syndrome (aids) and non-aids primary central nervous system lymphoma.primary central nervous system lymphoma (pcnsl) still occurs mainly in patients who are immunosuppressed, but its incidence is rising dramatically among immunocompetent individuals. the epstein-barr virus (ebv) has been detected by in situ hybridization in pcnsl tumor tissue from patients who are immunodeficient, but not from patients who are immunocompetent. using the more sensitive polymerase chain reaction (pcr) technique, the authors analyzed pcnsl tissue from 13 patients with acquired immun ...19921325277
stimulus-specific enhancement of luminol chemiluminescence in neutrophils by phosphatidylserine liposomes.when stimulated with different stimuli, neutrophils generate various active oxygen species. these active oxygen molecules can be analyzed by luminol chemiluminescence (lcl). phosphatidylserine (ps)-liposomes increased the formylmethionyl-leucyl-phenylalanine-induced lcl of guinea pig peritoneal neutrophils without affecting their oxygen consumption and superoxide (o2.-) generation. similar effects of ps-liposomes were also observed in lcl of neutrophils stimulated by phorbol myristate acetate or ...19921326254
the basolateral domain of the hepatocyte plasma membrane bears receptors for the circumsporozoite protein of plasmodium falciparum sporozoites.minutes after injection into the circulation, malaria sporozoites enter hepatocytes. the speed and specificity of the invasion process suggest that it is receptor mediated. we show here that recombinant plasmodium falciparum circumsporozoite protein (cs) binds specifically to regions of the plasma membrane of hepatocytes exposed to circulating blood in the disse space. no binding has been detected in other organs, or even in other regions of the hepatocyte membrane. the interaction of cs with he ...19921326407
5-ht4 receptor activation induces relaxation and associated camp generation in rat esophagus.changes in mechanical events and intracellular levels of camp induced by the activation of the 5-ht4 receptor were investigated in the rat esophagus tunica muscularis mucosae preparation. serotonin (5-ht) and 5 methoxytryptamine (5-mot; 5-ht4 agonist) caused concentration-related relaxation responses, while 5-carboxamidotryptamine (5-ct; 5-ht1 agonist), 1-(2,5-dimethoxy-4-iodophenyl)-2-amino-propane (doi; 5-ht2 agonist) and 2-methyl-serotonin (2-methyl-5-ht; 5-ht3 agonist) were less active. the ...19921326439
[potential-dependent ca2+ channels and contractile function of the heart in toxic-infectious shock caused plague].influence of intravenous administration to rats of murine toxin of y. pestis (1 mg/ml, ld100) on the number of potential-operated ca(2+)-channels, alpha- and beta-adrenergic and m1-cholinergic receptors of plasma membranes and heart contractility function has been studied in rats. the number of ca(2+)-channels in plasma membranes and contractility of heart decreased by 50% in 1 hour after the i.v. administration of toxin and further decreased up to the lethal end. during the agonal stage of the ...19921327279
[the plague: disease and vaccine?].plague has existed in madagascar since 1896, with epidemic control achieved by girard with an ev vaccine in 1937. plague persists in madagascar, however, due to the large animal reservoir. with a predilection for nodal tissues, yersinia pestis is a virulent bacteria that is potent inducer of antibody synthesis. immunity mechanisms stimulated by infection were studied: 1. in human by immunoenzymatic methods 2. in mice by seroprotection and vaccinating tests. induced immunity for people in endemic ...19921345094
bismuth subsalicylate in the treatment of h2 blocker resistant duodenal ulcers: role of helicobacter pylori.fifty nine patients with helicobacter pylori positive duodenal ulcers that failed to heal after a six week course of treatment with h2 blockers were randomly assigned to one of the following three regimens: (i) bismuth subsalicylate, 600 mg three times daily (n = 19), (ii) ranitidine, 300 mg at night (n = 20), (iii) bismuth subsalicylate plus ranitidine (n = 20). cumulative ulcer healing rates after four and eight weeks respectively were as follows: bismuth subsalicylate 74% (14/19) and 95% (18/ ...19921347278
chromosomal mapping of the high affinity fc gamma receptor gene. 19921347284
fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection.thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ...19921347287
[h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors].among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ...19921347325
hut 78 t cells bind to noncytokine-stimulated keratinocytes using a non-cd18-dependent adhesion pathway.the initial in vitro observation that cultured keratinocytes, when treated with cytokines such as gamma interferon, increased the binding of t lymphocytes, opened up a whole new avenue of research to understand epidermal trafficking patterns in inflammatory skin diseases. a growing body of data strongly supports the in vivo role of lymphocyte-function-associated antigen-1 (cd18) expression by t cells in the binding to intercellular adhesion molecule-1 (cd54) expressing keratinocytes. to further ...19921351368
availability of glycine and coenzyme a limits glycine conjugation in vivo.using benzoic acid as substrate, this study tested the hypothesis that capacity limitation of glycine conjugation in vivo is due to substrate-induced depletion of hepatic cosubstrates (i.e., atp, coenzyme a, and glycine) utilized in the conjugation reaction. benzolyglycine formation was investigated by following the disappearance of benzoic acid from blood and appearance of benzoylglycine in blood and urine after administration of sodium benzoate (0.2-2 mmol/kg, iv) to anesthetized rats whose ur ...19921352215
a decade of plague epidemiology and control in the western usambara mountains, north-east tanzania.outbreaks of human plague have been occurring in the western usambara mountains since 1980, involving many cases and deaths. epidemiological surveys and control activities were carried out from june 1980 to may 1990. rodents were trapped live, identified and serologically tested for plague, using the passive haemagglutination and passive haemagglutination inhibition tests. rodent fleas were collected, processed, identified and counted. house fleas were caught with light traps and similarly treat ...19921356303
genetic mapping of the murine gene and 14 related sequences encoding chromosomal protein hmg-14.the high-mobility-group chromosomal protein hmg-14 preferentially binds to nucleosomal core particles of mammalian chromatin and may modulate the chromatin configuration of transcriptionally active genes. the human gene for hmg-14 has been localized to the down syndrome region of chromosome (chr) 21 and may be involved in the etiology of this syndrome. here we show, by means of genetic linkage analysis of interspecific and intersubspecific backcross mice, that the murine functional gene, hmg14, ...19921360278
the role of nerve growth factor in collateral reinnervation by cutaneous c-fibres in the rat.we have investigated whether chronic nerve growth factor (ngf) depletion affects the development of transmedian collateral reinnervation by c-fibres. using a dye-labelled plasma extravasation technique in rats, the extent of transmedian innervation of the skin by c-fibres in the inferior alveolar nerve (ian) was determined 8-10 weeks after sectioning and preventing regeneration of the contralateral ian. another group of animals were immunised against ngf prior to the nerve section and a third gr ...19921360319
liver microsomal alkoxyphenoxazone o-dealkylases of white leghorn chickens: response to mixed function oxidase inducers.1. the cytochrome p-450 substrates, methoxy-, ethoxy-, pentoxy- and benzyloxyphenoxazone were investigated in liver microsomes of phenobarbital- and beta-napththoflavone-induced white leghorn chickens and compared to the rat mammalian model. 2. thirty-two-fold increases in benzyloxyphenoxazone o-dealkylase and 27-fold increases in ethoxyphenoxazone o-dealkylase activities were observed in beta-naphthoflavone-treated chickens compared to only an 18-fold increase in ethoxyphenoxazone o-dealkylase ...19921360354
eukaryotic gene regulation: simple vs complex models.the current generally accepted model of eukaryotic gene regulation is essentially a simple one. regulatory proteins containing separable dna binding and transcriptional activation domains, bind to specific dna sequences in promotors and interact directly or indirectly with the tata box binding factor to increase the rate of transcription initiation at selected promotors. here we present observations suggesting that the process may be more complex.19911367248
influence of system and molecular parameters upon fractionation of intracellular proteins from saccharomyces by aqueous two-phase partition.the interaction of molecular characteristics of proteins with the physicochemical properties of peg-phosphate aqueous two-phase systems has been studied. this has involved characterization of protein molecular weight, charge, and hydrophobicity and study of peg molecular weight and concentration, phosphate concentration, and ph. system characterization has been conducted in the context of limited stage fractionation procedures for protein recovery from baker's yeast. results are presented which ...19911367257
the organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function.we describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. the configuration of the construct has been engineered to maintain polarity and permit extended culture at the air-liquid interface. morphological, biochemical and kinetic parameters were a ...19921369170
characterization of zymomonas mobilis alkaline phosphatase activity in escherichia coli.zymomonas mobilis phoa gene encoding alkaline phosphatase was expressed in escherichia coli cc118 carrying the recombinant plasmid pzap1. the ph optimum for this enzyme was 9.0 and showed a peak activity at 42 degrees c. this enzyme required zn2+ for its catalytic activity; however, mg2+ or ca2+ significantly affected the activity. this enzyme was found to be ethanolabile, and ethanol inhibition was reversed by addition of zn2+. kinetics of z. mobilis alkaline phosphatase production in e. coli c ...19921369189
characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis.alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ...19921370473
termination of second messenger signaling in using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ...19921370581
biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis.the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ...19921370931
the vascular endothelial growth factor proteins: identification of biologically relevant regions by neutralizing monoclonal antibodies.angiogenesis plays critical roles in organ development during embryonic and fetal life, wound healing and in a variety of pathological conditions. vascular endothelial growth factor (vegf) is a secreted growth factor specific for vascular endothelial cells which induces angiogenesis in vivo. to gain a better understanding of the physiological role of vegf, we have generated and characterized four murine monoclonal antibodies (mabs) using the 165 amino acid species of recombinant human vegf as im ...19921380254
specificity of a tumor marker (ca54/61) and its individual epitopes recognized by monoclonal antibodies, ma54 and ma61, in human tumor patients.the specificity of a tumor marker (ca54/61) and its individual epitopes (ca54 and ca61) recognized by monoclonal antibodies (ma54 and ma61) and expressed by the same tumor marker were studied. serum levels of ca54 and ca61 were compared with that of ca54/61. in lung adenocarcinoma and ovarian carcinoma, the positive rates of ca61 (42% and 68%) were higher than those of ca54 (32% and 32%) and similar to those of ca54/61 (45% and 74%). the serum levels of ca54 and ca61 showed a significant correla ...19921383482
failure of intragastrically administered yersinia pestis capsular antigen to protect mice against challenge with virulent plague: suppression of fraction 1-specific antibody response.we evaluated the yersinia pestis capsular (fraction 1 [f1]) antigen as a potential oral immunogen in mice. we found that single doses of as much as 0.4 mg of f1, administered by intragastric (ig) intubation, were unprotective and did not stimulate production of detectable levels of specific antibody. three weekly ig doses resulted in low serum antibody levels that also did not provide protection against challenge with virulent y. pestis. assays of type-specific antibody following intubation and ...19921386197
a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells.members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ...19921391942
laboratory studies on yersinia pestis during the 1991 outbreak of plague in lushoto, tanzania.thirty-one specimens from patients involved in an outbreak of plague were cultured. suspicious isolates were presumptively identified by colonial characteristics, simple strains and by api 20e, and confirmed by inoculation into white mice. antimicrobial susceptibility testing was also done. the isolation rate of y. pestis was 22.6%. all isolates were uniformly sensitive to tetracycline, streptomycin, sulphadimidine and chloramphenicol; moderately sensitive to erythromycin, and resistant to trime ...19921404556
from the centers for disease control. pneumonic plague--arizona, 1992. 19921404753
fur regulation in yersinia species.the effects of iron have been linked with several phenomena including regulation of membrane proteins; however, the mechanism of iron regulation is not well characterized in yersinia pestis. it is well known that in escherichia coli, the fur gene product mediates negative transcriptional regulation of several genes in response to iron. we have cloned a y. pestis fur gene which is highly homologous to the e. coli fur regulatory gene. the sequence of the y. pestis fur gene exhibits 75% homology to ...19921406286
pneumonic plague--arizona, 1992.on august 26, 1992, a 31-year-old male resident of tucson, arizona, died of an illness subsequently diagnosed as primary pneumonic plague. this is the 10th case of plague reported in the united states in 1992, the first pneumonic plague case this year, and the first plague fatality reported since 1987 (cdc, unpublished data). this report summarizes the investigation of this case by county, state, and federal public health officials in arizona and colorado.19921406565
[plasmid heterogeneity in populations of yersinia pestis strains].yersinia pestis strains with the typical plasmid patterns were shown to have the heterogenic populations. heterogeneity is increased by cultivation passages in artificial nutrient media and is manifested in plasmid elimination within several clones, plasmid integration into the chromosome, appearance of auxiliary plasmids or the ones with increased molecular masses. passages of strains in experimental animals result in populations homogeneity with the typical plasmid patterns within all clones t ...19921406758
[the effect of the vole subspecies of the plague microbe on the viability of callopsylla caspia fleas].when infected with cultures of plague microbe reared at 28 c the experimental fleas showed no reliable differences in the mortality as compared to control ones. by contrast, the infection with agent cultures reared at 37 c as well as the infection on agonizing white mice caused a statistically reliable increase in the mortality in insects of both sexes. in the groups of fleas infected on white mice the most intensive mortality was observed during the first 24 hours after the infectious feeding t ...19921408363
[yersinia pestis adenylate cyclase and the deregulation of transmembrane signal transmission in the peritoneal leukocytes of white mice]. 19921414107
[antibiotics of the aminoglycoside group (gentamicin, sisomicin and amikacin) in the prevention and treatment of experimental plague].activity of aminoglycosides such as gentamicin, sisomicin and amikacin against plague microbe strains of natural origin was studied in vitro. it was also studied in prophylaxis and treatment of experimental plague infection in albino mice. the mac of gentamicin and sisomicin for 50 strains of the plague microbe was 0.2-1.6 micrograms/ml. for the majority of the strains it was 0.4 micrograms/ml. the amikacin mics were 0.4-3.2 and 0.8 micrograms/ml, respectively. high efficacy of gentamicin, sisom ...19921417325
effects of morphine on acquisition and maintenance of ethanol and water intake patterns in rats.two experiments were conducted to assess the effects of chronic subcutaneous injections of morphine (1.0 mg/kg) or saline on the pattern and amount of sweetened ethanol and water intake in fluid restricted long-evans rats. following daily injections, 2-h two-bottle choice tests were conducted with water and an ethanol solution (15% ethanol v/v in 5% sucrose w/v). during a 20-day acquisition phase (experiment 1), ethanol intake patterns and amounts did not differ between saline (n = 6) and morphi ...19921418670
development and optimization of an enzyme-linked immunosorbent assay employing two murine monoclonal antibodies for absolute quantitation of human beta-glucuronidase.we have developed and optimized an enzyme-linked immunosorbent assay (elisa) for absolute quantitation of human beta-glucuronidase. this is a double antibody sandwich system employing two murine monoclonal antibodies specific for human beta-glucuronidase developed in our laboratories. the method involves (a) coating of the high binding polystyrene microtitration plate with the first antibody (7b6 igg), (b) blocking of remaining active sites with 3% bovine serum albumin in phosphate-buffered sali ...19921418687
tumor necrosis factor and chemotherapeutic agents. potentiation of cytotoxicity with interferon gamma.this study examined combinations of the recombinant human cytokines, tumor necrosis factor alpha and interferon gamma, with doxorubicin and dactinomycin as well as other drugs on six squamous cell carcinoma cell lines of head and neck origin using the 3(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide proliferation assay. interferon gamma significantly enhanced the cytotoxicity of tumor necrosis factor alpha with dactinomycin on all six cell lines investigated, while in four of six cell ...19921418895
[sensitivity to mouse toxin and level of macrophage 5-nucleotidase activity].it is shown that immunomodulator of bacterial origin salmozan causes alternations of sensitivity to mouse toxin in mice cba. a correlation exists between the sensitivity to mouse toxin and the level of 5-nucleotidase of peritoneal exudate macrophages.19921421233
effect of buthionine sulfoximine and ethacrynic acid on cytotoxic activity of mitomycin c analogues bmy 25282 and bmy 25067.bmy 25282 and bmy 25067, analogues of mitomycin c (mmc), were synthesized in an attempt to increase the therapeutic potential of the parent drug. the present studies were undertaken to determine if the cytotoxicity of mmc or its analogues is affected by cellular glutathione (gsh) and/or gsh transferase (gst) levels by using sensitive (p388/s) and multidrug resistant (p388/r-84) mouse leukemia cells as a model. p388/r-84 cells were cross-resistant to all three drugs. bmy 25067 was > 100 times mor ...19921423311
prevalence of ompt among escherichia coli isolates of human origin.ompt is a protease associated with the outer membrane of escherichia coli and possesses a high degree of homology to the plasminogen activator, pla, of yersinia pestis. we show here that ompt from intact cells can indeed activate plasminogen. clinical specimens of e. coli were examined for protease activity and for the ompt gene. few isolates (12%) were found to be positive for ompt activity, whereas most (77%) carried the ompt gene and expressed the cloned protease gene. in this report we prese ...19921427004
possible targets on carcinoma for bmab retargeting of lymphocyte or drug cytotoxicity.retargeting of drugs or lymphocyte cytotoxicity through bi-specific monoclonal antibodies (bmabs) has been proven a therapeutic tool against human carcinoma both in pre-clinical in vitro and in vivo studies. some of these reagents have already been introduced into clinical trials and preliminary results appear to be promising. however, improvement of the specificity of this approach could be achieved by selecting more suitable target molecules on tumor cells. the research focused on developing m ...19921428402
novel surface molecules involved in human nk cell activation and triggering of the lytic machinery.three new monoclonal antibodies (mabs) termed 7a6, pp35 and a6/143 were isolated after mouse immunization with cd3- cd16+ nk clones. the screening procedure was based on the ability of mabs to trigger cytolytic activity of the immunizing clones in a re-directed killing assay against the p815 murine mastocytoma cell line. the 7a6 mab reacts with 58 kda surface molecules that appear to belong to the same molecular family defined by the previously described nk-sub-set-specific gl183 and eb6 mabs. h ...19921428407
characterization of phosphofructokinase-deficient canine erythrocytes.dogs homozygously affected with muscle-type phosphofructokinase (pfk) deficiency had about 20% of normal erythrocyte pfk activity and exhibited a compensated haemolytic anaemia. erythrocyte glucose-6-phosphate and fructose-6-phosphate concentrations were increased and dihydroxyacetone phosphate and 2,3-bisphosphoglycerate values were below normal in affected dogs. other intermediates distal to the pfk step were not significantly below normal and fructose-1,6-bisphosphate was even above normal. e ...19921434514
a surface protease and the invasive character of plague.a 9.5-kilobase plasmid of yersinia pestis, the causative agent of plague, is required for high virulence when mice are inoculated with the bacterium by subcutaneous injection. inactivation of the plasmid gene pla, which encodes a surface protease, increased the median lethal dose of the bacteria for mice by a millionfold. moreover, cloned pla was sufficient to restore segregants lacking the entire pla-bearing plasmid to full virulence. both pla+ strains injected subcutaneously and pla- mutants i ...19921439793
[role of histamine receptors of mouse and guinea pig peritoneal leukocytes in the pathogenetic effect of yersinia pestis adenylate cyclase].the effect of purified preparation of adenylate cyclase isolated from y. pestis on peritoneal leukocytes of white mice and guinea pigs was investigated. y. pestis adenylate cyclase was shown to accomplish its pathogenic action via histamine-specific receptors on the surface of eukaryotic cells. the involvement of h1 and h2 histamine receptors on target cells in the adenylate cyclase action leading to development of plague infection is discussed.19921446035
loss of the pigmentation phenotype in yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal dna which is flanked by a repetitive element.the pigmentation (pgm+) phenotype of yersinia pestis encompasses a variety of different physiological traits, all of which are missing in pgm- mutants. we have previously shown that loss of the pgm+ phenotype is accompanied by the spontaneous deletion of at least 45 kb of chromosomal dna, referred to as the pgm locus. using chromosomal walking, we have now mapped the full extent of the pgm locus in y. pestis strain kim6+. our results indicate that the locus spans 102 kb of dna which is absent in ...19921447977
b and t cell leakiness in the scid mouse mutant.recombination of antigen receptor gene elements (v, d and j) that code for ig and tcr variable regions is defective in the scid mouse mutant. however, the defect is not absolute. developing scid lymphocytes occasionally succeed in forming a productive vdj and vj rearrangement at two critical loci (e.g., igh/igl or tcr beta/tcr alpha), and given the appropriate stimuli, differentiate into clones of functional b or t cells. scid mice with detectable b and/or t lymphocytes are referred to as leaky. ...19921449786
yersinia pestis yopm: thrombin binding and previous studies, yersinia pestis yopm has been shown through mutational analysis to be necessary for virulence in mice and found to have homology with the thrombin-binding domain of the platelet receptor gpib alpha. in this study, yopm was purified and shown by dot blot and chemical cross-linking tests to bind to human alpha-thrombin. no cross-linked product could be detected when human prothrombin was incubated with yopm. as a functional test of thrombin binding, it was shown that native bu ...19921452357
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