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chromosomal locations of mouse immunoglobulin genes.the chromosomal locations of the structural genes coding for the constant portions of mouse heavy (h) and light chain immunoglobulins were studied by molecular hybridization techniques. complementary dna probes containing the constant-region sequences of kappa and lambdai light chain and alpha, gamma2b, and mu heavy chain mrnas were annealed to a large excess of dna from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. the lines were scored as pos ...197896442
effects of ethanol on pregnant rats and their offspring.pregnant rats were intubated with either 1.0 or 2.0 g/kg of ethanol daily throughout gestation. pair-fed vehicle-treated, and nontreated rats fed ad libitum, served as control groups for ethanol-treated animals. ethanol treatment reduced food and water consumption and attenuated the gain in body weight of pregnant animals relative to nontreated animals fed ad libitum. litter size, litter weight, and the mean weight per pup were reduced in both the ethanol-treated and pair-fed control groups. the ...197896469
[oesophageal diverticulum in a pony (author's transl)].a case of oesophageal diverticulum in a pony is reported. the diagnosis was established by clinical and x-ray examination (following administration of a contrast agent) and verified by post-mortem studies. the cause and pathogenesis are discussed.197896547
haplotype designations of various strains of guinea pigs. 197896556
[biological properties and mechanisms of development of bacteria resistant to fusidin, erythromycin and chloramphenicol]. 197896638
phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver.rat liver phospholipids were radioactively labeled in vivo before purification of udp-glucuronyltransferase to homogeneity. the pure enzyme contained very little phospholipid (approx. 0.7 mol of phospholipid/mol of protein). the solubilization detergent lubrol 12a9 appeared to act as a phospholipid substitute, capable of supporting udp-glucuronyltransferase activity. phospholipase c did not inhibit the pure enzyme activity and pure udp-glucuronyltransferase was stimulated by 40--100% by the addi ...197896816
5-thio-d-glucose is an acceptor for udp-galactose : d-glucose 1-galactosyltransferase. 197896819
2-aminoethylphosphonic acid as an indicator of tetrahymena pyriformis w growth in protein-quality evaluation assay.1. the concentration of 2-aminoethylphosphonic acid (aep) in 96 h cultures of tetrahymena pyriformis w was studied in order to apply it as an indicator in the assay of the relative nutritive value (rnv; protozoa population with test protein:protozoa population with whole-egg powder) of protein. foodstuffs and food mixtures of different protein contents and qualities were used as test samples. 2. rnv values based on aep determination (rnvaep) were compared with corresponding values calculated fro ...197896851
phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans.stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ...197896857
comparison of effects of normal rabbit sera and anti-cock sperm sera on rabbit sperm, including comparison of effects on the sex ratio. 197896880
[radioimmunological aspects of neuropeptides].immunological aspects of neurosecretory peptides are relevant to antibody generation, labelling of peptides for radioimmunoassays and in particular the metabolism of neuropeptides. antibodies were generally produced by adsorption or polymerization (using different approaches and various coupling agents) of peptides with intrinsic antigenic molecules preceding their administration to recepient animals (rats, guinea pigs, rabbits and sheep) with adjuvants. antibodies were also obtained by injectin ...197896897
[formation of cutaneous nerve trunks in the chick. ultrastructural and quantitative analysis].in chick skin structuration of nervous trunks takes place at 14-15 days of incubation, at the time of formation of the adult pattern of cutaneous innervation. at this same stage, myelogenesis begins, but develops mainly after hatching: only 4% of axons are myelinated at hatching, 40% in 6-week old chickens and 60% in adults. this "critical" stage (14-15 days of incubation) apparently corresponds to the stabilization of cutaneous nerve supply.197896985
[nature of the mutations that determine the ability of bacillus subtilis a-50 to sporulate at high glucose concentrations in the medium].the ability of bacillus subtilis a-50 to sporulate in the medium containing high glucose concentrations is caused by at least two mutation types: pts mutations and cat (or tgl) mutations, both of them affecting differently the level of alkaline proteinase synthesis. the decrease of the level of enzyme activity in the case of pts mutation (glur3 mutant) occurs at the expense of glucose transport disturbance. the mutation cat (tgl) (mutant glur5) causes the increase in enzyme synthesis at the expe ...197897172
biosynthesis of delta-aminolevulinic acid by blue-green algae (cyanobacteria).when levulinic acid, a competitive inhibitor of delta-aminolevulinic acid dehydratase, was added to growing cultures of blue-green algae (cyanobacteria), delta-aminolevulinic acid was excreted into the medium and cell growth was inhibited.197897274
amino acid sequence of beta-galactosidase. viii. sequence of the nh2-terminal segment, cnbr peptides 1 to 9, residues 1 to 377.the amino acids in 9 cyanogen bromide peptides have been placed in sequence starting from the nh2 terminus. the peptides account for residues 1 to 377 of the whole protein and include the largest (cnbr7, 119 residues) and the smallest (cnbr1, 2 residues) of the cyanogen bromide peptides. this region contains only 3 of the 20 lysine residues in the polypeptide chain. a high proportion of charged groups are present (28 of 66 arginine, 28 of 60 glutamic acid, and 24 of 65 aspartic acid residues).197897295
preliminary x-ray crystallographic data on phospholipase c from bacillus cereus. 197897390
increased vasodepressor effect of prostaglandins a1 and e1 in renal hypertensive rabbits. 197897482
malignant transformation of a mouse epithelial cell line with polycyclic aromatic hydrocarbons. 197897564
functional specialisation in the visual cortex of the rhesus monkey.anatomical and functional studies of the visual cortex of the rhesus monkey have shown that it is made up of a multiplicity of distinct areas. these seem to be functionally specialised to analyse different features of the visual environment.197897565
the effect of prolonged dextran administration in guinea pigs.dextran 70 6% in 0.9% nacl solution, injected intraperitoneally in guinea pigs, 10 ml/kg b.w./day, during a period of 27 days brought the lowering of the plasma proteins level, whie the total colloid osmostic pressure (due to dectran and proteins) remained close to that of the control group. the concentration of typhus 0 antibodies and the number of precipitation lines assayed during immunodiffusion in gel were lower than in thd control group. the diminishing of the red blood cells number and of ...197897691
[reservoirs and wild vectors of trypanosoma cruzi. lxviii: infection of mammals by oral route]. 197897735
isolation care of infection-prone burn patients.an experimental and clinical study of spread of colonisation between burn patients, and their susceptibility to infection, was performed. burn patients' polymorphonuclear neutrophil granulocytes (pmn) functioned poorly, particularly during the second week after injury which coincided with maximum growth of bacteria in the burn wound. patients with large burns often dispersed s. aureus and ps. aeruginosa but also beta-hemolytic streptococcus to the air of their rooms. airborne transfer of these b ...197897776
the pattern of sporulation in anabaena circinalis and comments on the role of heterocysts in sporulation in blue-green algae.cells between two intercalary heterocysts differentiate at random into spores in a. circinalis. one or more cells, which fail to transform into spores, are present between the two adjacent spores, and these cells disorganize later. a critical c:n ratio regulates sporulation and heterocyst formation. during sporulation the reductive ability of the heterocyst gradually diminishes. it is concluded on the basis of this and other evidence that sporulation is regulated by interactions between heterocy ...197897869
genetics of the idiotype of strain akr antibodies to group a streptococcal carbohydrate; further evidence for a low degree of homology in the vh chromosomal region.a new vh gene is described which governs the expression of idiotypes associated with the antibody response of strain akr mice to group a streptococcal carbohydrate (a-cho). this vh gene, termed achd, is identified using guinea pig antisera to a pool of strain akr antibodies to a-cho. the genetic polymorphism is revealed by quantitative differences in idiotype expression between different inbred strains. in recombinant inbred strains linkage to the ig-1d allotype allele of strain akr is demonstr ...197897871
activity of three 8-hydroxyquinoline derivatives against in vitro dental plaque.three 8-hydroxyquinoline derivatives, assessed using an in vitro preformed dental plaque model system, were differentially inhibitory for four plaque-forming microorganisms.197898105
characteristics of pr5, a lipid-containing plasmid-dependent phage.an extensive characterization of plasmid-dependent phage pr5 isolated from sewage has been carried out. the phage has a head diameter of 65--68 nm, is isometric with a double-layered capsid, and a minority possess tails. it adsorbs to many but not all types of bacteria possessing p, n, or w plasmids. the phage contains 20% lipid, 15.1% dna, and 64.9% protein by weight and has a buoyant density of 1.265 g/ml in cscl. the dna is double-stranded with a g + c content of 49% and a molecular weight of ...197898225
cellular uptake, transport, and macromolecular binding of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene by human cells in vitro. 197898231
behavioral observations in squirrel monkeys (saimiri sciureus) following methylmercury exposure. 197898322
ultrastructural hepatic changes by carbamate pesticide (sevin) in rats. 197898323
biochemical changes in guinea pig lungs due to amosite asbestos. 197898326
immunopathology of malaria. 197898476
formylation of initiator trna methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression.eucaryotic and procaryotic organisms differ in two aspects of their translation machinery: polycistronic messengers are expressed as a sequence of individual proteins only in procaryotes, and the initiation of protein synthesis proceeds with an initiator trna which is found to be modified (formylated) in procaryotes and not in eucaryotes. in the present study, we show that formylation is required in vivo for the coordinate expression of the escherichia coli lactose operon. our experiments are co ...197898518
immunization with dextransucrases, levansucrases, and glycosidic hydrolases from oral streptococci. ii. immunization with glucosyltransferases, fructosyltransferases, and glycosidic hydrolases from oral streptococci in monkeys.the feasibility of immunizing monkeys with enzymes from oral streptococci in an attempt to reduce dental caries was investigated. forty rhesus monkeys, macaca mulatta, were used. cariogenic streptococci, s mutans, were implanted into all the monkeys' mouths. there was no pathological effect resulting from immunization. of the 40 animals, 30 retained the implanted flora throughout the experiment; the remaining 10 were reimplanted until the streptococci remained. in six months, gross carious lesio ...197798540
purification and some properties of ovine placental lactogen.a method has been described for the purification of ovine placental lactogen (opl) involving the use of freshly obtained sheep foetal cotyledons. tissue was extracted with 0.1 m-ammonium bicarbonate and the supernatant fraction, adjusted to ph 7, was brought to 60% saturation with ammonium sulphate. the resulting precipitate was then subjected to a sequence of chromatographic steps using columns of sephadex g-100 and carboxymethylecellulose. during each stage of the purification, the lactogenic ...197898606
the effects of sodium nitroprusside and trimethaphan camsylate on cerebral blood flow in rhesus monkeys.hemispheric cerebral blood flow was measured in the rhesus monkey before and after infusion of the hypotensive agents sodium nitroprusside and trimethaphan camsylate. the intracarotid injections of 133xe was utilized, and flow was calculated by the "flow initial" technique. cerebral blood flow did not change significantly with the administration of trimethaphan camsylate. however, with a small reduction in blood pressure (10.6%) during the administration of sodium nitroprusside, the cerebral blo ...197898729
location of the genes for human heavy chain immunoglobulin to chromosome 6.immunoglobulin synthesis was examined in 31 man-mouse hybrid clones produced by fusing rag mouse cells with human lymphoid cells. cells were grown in serum-free medium containing [(14)c]leucine and a (14)c-labeled amino acid mixture. spent medium was dialyzed, concentrated, and subjected to radioimmunoelectrophoresis. eighteen clones were found to produce material that gave a radiolabeled precipitin line with anti-human igg (gamma-chain specific). production of material which was indistinguishab ...197898768
frequency and epidemiology of primary drug-resistance in tuberculosis in the federal republic of germany including berlin (west). partial result of a study of the w.a.t.l. 197898839
vascular dynamics of an experimental cerebral arteriovenous shunt in the primate.an arteriovenous fistula which shunted arterial blood from the circle of willis to the internal jugular vein was created in ten monkeys. continued patency of the shunt was confirmed angiographically. cerebral blood flow measurements were then obtained with the shunt both open and occluded. flow was also determined in response to elevations of systemic arterial pressure and raised arterial carbon dioxide tensions (paco2), again with the shunt both patent and occluded. in addition, flow through th ...197898854
[some aspects relating to the aflatoxin generation during the self-heating of cereals].in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...197898933
jaundice and breast-feeding among alaskan eskimo newborns.the course, incidence, and severity of neonatal jaundice was studied in 95 alaskan eskimo infants. breast-fed infants had higher bilirubin concentrations than bottle-fed babies. both groups experienced high bilirubin levels, similar to those previously reported in navajo and oriental infants but greater than those observed in whites and blacks. a marked capacity to inhibit hepatic glucuronyl transferase was observed in breast-milk specimens but only partly accounted for the bilirubin differences ...197899026
pars plana vitrectomy as a primary treatment for acute bacterial endophthalmitis.we successfully treated six patients with culture-proven acute bacterial endophthalmitis by a combination of emergency pars plana vitrectomy and the instillation of intravitreal antibiotics. all six patients regained satisfactory vision.197899040
interferon inducing activity of polyinosinic acid.although poly(i) is generally considered to be inactive as an interferon inducer, we have found several authentic poly(i) preparations to be effective inducers. their interferon inducing ability varied considerably from one cell system to another. in human diploid fibroblasts, primed with interferon and superinduced by cycloheximide and actinomycin d, all active poly(i) samples proved nearly as effective in inducing interferon as poly(i).poly(c). in primary rabbit kidney cell cultures, the activ ...197899486
lumbar spondylectomy in the subhuman primate.removal of the 2nd or 3rd lumbar vertebrae (or both) has been accomplished in the subhuman primate (macaca mulatta). variations between this animal and the dog in posture, vertebral column anatomy, and spinal cord blood supply made no apparent difference in the results when compared with those in previous experiments. all macaques were able to clinb and to use their hind legs in a normal manner within 3 days after surgical operation. once hair had regrown over the surgical incision, persons not ...1978100028
rosette formation by human lymphoid cells with monkey red blood cells.the present paper describes a study on rosette formation by human lymphoid cells with monkey erythrocytes (mrbc) obtained from two species: african green monkey (cercopithecus aethiops) and rhesus monkey (macaca mulata). all the lymphocyte preparations obtained from peripheral blood, tonsils, thymocytes, bone-marrow and spleen biopsies formed rosettes in variable proportions with erythrocytes obtained from these two monkey species tested. cells of established lymphoblastoid cell lines characteri ...1978100044
[method of determining the antimicrobial activity of alcohol extracts of propolis].it is proposed to determine the antimicrobial activity of propolys alcohol extracts by the method of subsequent dilutions in solid nutrient media. dilution of the extracts immediately in hot agar eliminated the inhibiting effect of the extragent on the microbial growth. opalesence appearing in the agar did not prevent estimation of the results in contrast to the method of subsequent dilutions in liquid nutrient media.1978100047
the distribution of hydrolytic enzyme activities in human fibroblast cultures and their intercellular transfer. 1978100108
[origin of a mitogrenic factor for cultivated macrophages (imf: inflammatory mitogenic factor) found in exudates of non-specific acute inflammation].the mitogenic activity of inflammatory exudate obtained from irradiated rats is reduced. after transfer of bone marrow syngeneic cells into irradiated rats this mitogenic activity is further decreased, while after transfer of thymic cells it is increased. it is postulated that the mitogenic activity of inflammatory exudate could be related to thymic cells and that t lymphocytes may be involved in non specific-inflammatory reactions.1978100246
occurrence of squalene and sterols in cellulomonas dehydrogenans (arnaudi 1942) comb. nov. hester 1971.the neutral lipid fraction of the photochromogenic, coryneform bacterium cellulomonas dehydrogenans (arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. the compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. de novo sterol biosynthetic ability was shown from incorporation of 14c from d-[u-14c]glucose into squalene and the sterol fraction. the squalene concentration ...1978101527
squirrel monkey temporary threshold shift from 48-h exposures to low-frequency noise.five squirrel monkeys were exposed for 1, 2, 4, 8, 16, 24, and 48 h to a 375--750-hz band noise at an overall spl of 95 db. the tts4.5 growth pattern for the 750-hz test frequency was biphasic and did not reach an asymptote after 48 h of exposure. for all exposures, the mean thresholds of the five monkeys returned to within 5 db of the preexposure mean 20 h after exposure. recovery curves from all exposures at the 750-hz test frequency appeared biphasic. increasing spl from 95 to 105 db increase ...1978101572
crystallographic data on a complete kappa-type human bence-jones protein. 1978101675
studies on virus-inhibiting substances of bacterial origin. iv. antiviral substances extractable from streptococcus faecalis: its in vivo effect. 1978101714
[the effect of prolonged irradiation and of certain radioprotective agents on glycosaminoglycans in the rat]. 1978101997
temporal relationship of translation and glycosylation of immunoglobulin heavy and light chains.the initial glycosylation of mpc 11 gamma 2b heavy chains occurs quantitatively in vivo when the nascent heavy chains reach a size of approximately 38 000 daltons. nonglycosylated, completed mpc 11 heavy chains cannot be glycosylated in these cells. other classes of mouse heavy chains (i.e., mu, alpha, and gamma 1) also appear to be glycosylated as nascent chains; nonglycosylated, completed heavy chains cannot be glycosylated by the cell in any of these cases. in contrast, variant mpc 11 cells s ...1978103571
single-unit activity in the auditory cortex of monkeys selectively attending left vs. right ear stimuli.single-unit responses were recorded from the auditory cortex of rhesus monkeys that were performing an auditory selective attention task. acoustic stimuli were presented randomly to either the left or right ear through headphones. in a given block of trials one ear was selected as the ear to be attended, and was indicated by lighting either a left or right response key. the animal's task was to press the lighted key whenever stimuli were presented to the attended ear, but to make no response to ...1978103597
organization of the sympathetic innervation in liver tissue from monkey and man.the sympathetic innervation of the liver of monkey and man has been investigated in a combined fluorescence histochemical, chemical and electron microscopical study. by means of the falck-hillarp fluorescence method a dense network of monoamine-containing nerve fibers was visualized in liver tissue of monkey and man. the nerve fibers ran in close contact to both hepatocytes and blood vessels. chemical quantitations showed high concentrations of noradrenaline in both human and monkey liver. micro ...1978103622
oligosaccharides and polysaccharides specifically utilizable by bifidobacteria. 1978103638
[effect of lymphokines produced in a grafted system on production of prostaglandins by macrophages].mice peritoneal macrophages cultured in vitro release a prostaglandin-like activity as evaluated by a bio-assay using rat stomach fundus. the prostaglandin release is greatly increased when macrophages are incubated with the supernatant of mixed lymphocyte cultures between recipient and donor of a skin allograft. this phenomenon was found in all strains of mice tested except c3h/hej mice, a strain already known for its defective responsiveness to bacterial lipopolysaccharide (lps).1978103646
[survival capacity of certain pathogens under environmental conditions]. 1979103703
a receptor for polymerized human and chimpanzee albumins on hepatitis b virus particles co-occurring with hbeag.a receptor for polymerized human serum albumin was demonstrated on dane particles as well as on 20-nm hepatitis b surface antigen particles, isolated from asymptomatic carriers of hepatitis b virus who were positive for hbeag. in contrast, such receptor was not born by 20-nm hepatitis b surface antigen particles obtained from carriers positive for antibody to hbeag. hepatitis b surface antigen particles with the receptor were heavier than those without, and when treated with pronase, they became ...1979103774
dietary lactose and the aetiology of human small-intestinal hypolactasia. 1978103781
on the interactions of yersinia strains and cell cultures.comparative investigations were carried out on the dynamics of invasion of cell cultures by strains yersinia pestis ev and yersinia pseudotuberculosis treated vitally with petroleum ether. it was established that the invasiveness of the strains studied was related to the presence of chemical structures of the bacterial surface sensitive to the action of either and whose synthesis was temperature dependent. the penetration and initial stages of intracellular multiplication of the yersinia were ac ...1978104477
[accelerated diagnostic cultivation of pathogenic environmental microbes. ii. accelerated diagnostic cultivation of the causative agents of anthropozoonotic plague, glamders, melioidosis, brucellosis and tularemia]. 1978106632
inhibition of neisseria gonorrhoeae by normal human saliva.saliva was found to be a powerful and specific inhibitor of neisseria gonorrhoeae. although 28 other species of bacteria were tested, including neisseria meningitidis, neisseria pharyngis var flava, neisseria lactamica, and neisseria catarrhalis, we failed to find any others sensitive to saliva under similar conditions. the physical properties of the inhibitory substance indicated that it might be salivary alpha-amylase. to test this hypothesis alpha-amylase was extracted from saliva and was sho ...1979106919
production, purification, and properties of dextransucrase from leuconostoc mesenteroides nrrl b-512f.the production of dextransucrase from leuconostoc mesenteroides nrrl b-512f was stimulated 2-fold by the addition of 0.005% of calcium chloride to the medium; levansucrase levels were unaffected. dextransucrase was purified by concentration and dialysis of the culture supernatant with a bio-fiber 80 miniplant, and by treatment with dextranase followed by chromatography on bio-gel a-fm. a 240-fold purification, with a specific activity of 53 u/mg, was obtained. contaminating enzyme activities of ...1979106966
liver transplantation. 1979107000
effect of human serum thymic factor on immature t lymphocytes in acute leukemia and other hematologic malignancies.in 37 patients with acute leukemia and in 13 patients with other hematologic malignancies, e-rosette formation of peripheral blood mononuclear cells, before and after incubation with human serum thymic factor, was studied. this assay showed that incubation with thymic factor caused a clear-cut increase of e-rosette-forming cells in 8 of the 37 acute leukemia patients. among the 13 patients with other hematologic malignancies, a similar effect was observed in two with pediatric hodgkin's disease ...1978107146
acetylated methylmannose polysaccharide of streptomyces griseus. locations of the acetyl groups.the positions of esterification of the 4 to 5 acetyl residues in the acetylated methylmannose-containing polysaccharide from streptomyces griseus have been established by the methyl replacement technique, wherein ester substituents are specifically replaced with methyl ether substituents. the newly incorporated methyl groups were distinguished from 3-o-methyl groups by the use of polysaccharide containing radioactively labeled endogenous methyl groups. the positions of methyl group localization ...1979107171
effects of dietary composition on somatomedin activity in growing rats. 1979107298
[preparation of extracellular ribonuclease from bacillus subtilis].the paper describes a method for isolating alkaline ribonuclease from the culture liquid bacillus subtilis kp 349 which involved: submerged cultivation of the producer on complex and synthetic nutrient media with optimized rnase activity, acid treatment of the total culture liquid, and filtration through perlite. further treatment may include either spray drying of the culture liquid filtrate or its concentration in a vacuum evaporator, dialysis of the concentrate against distilled water, and d ...1978107517
fat-induced jejunal inhibition of gastric acid secretion and release of pancreatic glucagon, enteroglucagon, gastric inhibitory polypeptide, and vasoactive intestinal polypeptide in man.the effect of intrajejunal (i.j.) infusion of fat on meal-stimulated gastric acid secretion and release of pancreatic glucagon (pg), enteroglucagon (eg), gastric inhibitory polypeptide (gip), and vasoactive intestinal polypeptide (vip) was studied in seven healthy volunteers. i.j. fat markedly inhibited meal-stimulated acid secretion as compared to a control study with i.j. saline infusion. the acid inhibition was accompanied by augmental plasma concentrations of eg, gip, and vip but not of pg, ...1979107575
frequency and fate of human renal allografts contaminated prior to transplantation.bacterial cultures were done from donor ureter and/or graft perfusion fluid on 92 of 112 patients who had renal transplantations during 1977. three samples of perfusion fluid and 14 samples of donor ureter contained bacteria. in nine cases there was a gram-positive organism, and in 10 cases a gram-negative rod was (among) the contaminant(s). there was no difference in the rate of graft survival, patient survival, or in the frequency of major postoperative complications between the two groups. on ...1979107605
the viability of cryopreserved aegyptianella pullorum carpano, 1928 in the vector argas (persicargas) walkerae kaiser and hoogstraal, 1969. 1979107668
reduced uptake of oestrone and corticosterone by cells from cirrhotic rat liver. 1979107697
adverse hemodynamic and ultrastructural changes in dog hearts subjected to protein-calorie malnutrition.in the absence of thiamine deficiency, the specific effects of protein-calorie malnutrition on left ventricular (l.v.) function are unknown. mature beagle dogs of both sexes were subjected to a hypocaloric, nitrogen-poor diet which resulted in a weight loss of approximately 40% after seven weeks. following preparation of this nutritional model, myocardial contractility was assessed acutely by obtaining isovolumetric l.v. contractions on cardiopulmonary bypass at constant heart rate, mean aortic ...1979107775
spinal cord evaluation by cortical evoked responses.in ten monkeys, selective segmental lesions of the dorsal columns at the upper thoracic and middle cervical levels resulted in almost total attenuation of the cortical evoked potential responses to peripheral nerve stimulation. conversely isolated segmental dorsal column preservation showed intact transmission of the evoked responses at rostral spinal cord, nucleus ventralis posterior lateralis, and cortical levels. responses recorded from the intralaminar thalamic nuclei in the region of nucleu ...1979107931
[in vitro biosynthesis of beta-lipotropin in brain tissue].we have recently developed methods to identify biosynthetized beta-endorphin and beta-lipotropin (beta-lph) following incubation of rat pars intermedia with radioactive amino acids. we used the same approach for rat brain tissue. in the striatum we found a peptide similar to beta-lph while its identification in hypothalamus was less positive. this is the first demonstration of such biosynthesis and it could well be an important step in determining the biosynthetic patterns of cerebral endorphins ...1979108023
fascioloides magna: development in selected nonruminant mammalian hosts. 1979108129
accumulation of iron by yersiniae.escherichia coli, bacillus megaterium, and three species of yersiniae grew rapidly without significant production of soluble siderophores in a defined iron-sufficient medium (20 microm fe3+). in iron-deficient medium (0.1 to 0.3 microm fe3+) all organisms showed reduced growth, and there was extensive production of siderophores by e. coli and b. megaterium. release of soluble siderophores by yersinia pestis, y. pseudotuberculosis, or y. enterocolitica in this medium was not detected. citrate (1 ...1979108243
isolation and characterization of bacterial flagellar hook proteins from salmonellae and escherichia coli.flagellar hook proteins from salmonella and escherichia coli were dissociated in acid and purified by diethylamino-ethyl-cellulose column chromatography. these two proteins had the same electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels. however, analytical electrofocusing patterns showed that these proteins had different isoelectric points (4.7 for salmonella typhimurium and 4.4 for e. coli). immunodiffusion and immuno-electron microscopy carried out with antisera prepared a ...1979108251
tuberculosis of the mandible presenting as 'lumpy jaw'. 1979108413
identification of 15-keto-9, 11-peroxidoprosta-5, 13- dienoic acid as a hematin-catalyzed decomposition product of 15-hydroperoxy-9, 11-peroxidoprosta-5, 13-dienoic acid.a labile prostaglandin was isolated as one of the products generated from [1-14c] eicosatetraenoic acid incubated with sheep vesicular gland microsomes. the eicosatetraenoic acid metabolite amounted to ca. 16% of the total radiolabeled products. formation of this new prostaglandin was prevented when heat-denatured microsomes were employed or when incubation mixtures were supplemented with indomethacin or phenol. however, incubation of prostaglandin g2 (pgg2) with hematin in the presence or absen ...1979108495
pantothenase. 1979108511
dietary modification of amphetamine stereotyped behaviour: the action of tryptophan, methionine, and lysine.rats fed diets high in tryptophan, methionine, and tryptophan plus methionine or lysine were subsequently injected with 10 mg/kg d-amphetamine. the amount of amphetamine-induced stereotyped behaviour observed varied as a function of the dietary addition, with methionine showing the greatest difference to controls, and lysine the least. whole brain catecholamine levels and 5-hydroxytryptamine levels also showed amino acid-specific changes.1979108724
evidence of a high-activity c type of carbonic anhydrase in human ciliary processes.carbonic anhydrase activity was found in the ciliary process of fresh human donor eyes, originating from an enzyme antigenically similar to the erythrocyte high-activity isoenzyme hca c. it was sensitive to inhibition by acetazolamide and resistant to inhibition by halides like hca c. the enzyme is probably identical with hca c. its tissue concentration was one fifth to one tenth of that in the human kidney. the erythrocyte low-activity isoenzyme hca b was also found in the processes as a contam ...1979110722
lethal effect of protamine and histone on competent bacillus subtilis cells. inhibition of genetic transformation by protamine in sublethal concentration.under experimental conditions of genetic transformation, protamine and total histone were bactericidal for bacillus subtilis cells. the abilities to cause lethality were very similar for both, either protamine or histone, with no antagonistic effects amongst these natural polycations. with both basic proteins acting simultaneously the enhancement was higher than a summation of the separate lethal effects. sublethal concentration of protamine added at the beginning of transformation time, produce ...1979111002
[melanin-producing strains of pseudomonas aeruginosa]. 1979111005
evaluation of high-protein supplements in children with protein energy malnutrition.the present study was designed to test the comparative efficacy of cow's milk, a protein, vitamin and mineral mixture (pvm) and soybean textured vegetable protein (somos) as supplements to maize meal porridge in the dietary treatment of children with protein energy malnutrition (pem). each diet was designed to provide approximately 480 kj/kg with 2 protein/kg from the test protein, and 1,8 g protein/kg from maize. response to treatment was measured by loss of oedema, subsequent weight gain, rate ...1979111358
cerebellar cortical atrophy in an adult japanese macaque. 1979111404
neutralizing influenza antibodies, iga and total protein in the nasopharyngeal secretions of subjects vaccinated by nasal route with the inactivated influenza vaccine prepared in the "stefan s. nicolau" institute of virology.intranasal administration of two doses of the inactivated influenza vaccine prepared in the "stefan s. nicolau" institute of virology was followed by rises in the level of neutralizing secretory influenza antibodies in 82% of the cases. the concomitant study of secretory antibody, iga and total protein levels, as well as of the serum hai influenza antibodies demonstrated that their evolution was parallel only in 23% of the vaccinees. the percentage of secretory antibody conversion was similar to ...1979111408
[bactericidal effect of disinfectants on various strains of mycobacterium tuberculosis]. 1979113848
pharmacological modification of experimental tardive dyskinesia.cebus apella monkeys subjected to chronic haloperidol administration develop neurologic disturbances very similar to neuroleptic-induced acute dystonia human beings. after varying lengths of time, certain monkeys develop a prolonged dyskinetic syndrome resembling tardive dyskinesia (td), as seen clinically. two monkeys with signs of td were given single intramuscular injections of various compounds with known effects on the catecholaminergic, cholinergic, serotoninergic and gaba-ergic neurotrans ...1979115227
pancreatic transplantation in cases of diabetes complicated by renal insufficiency. 1979115240
the primer trna for moloney murine leukemia virus dna synthesis. nucleotide sequence and aminoacylation of trnapro.the nucleotide sequence of a trna primer molecule for initiation of moloney murine leukemia virus dna synthesis has been determined. the sequence is heterogeneous in two positions but both forms, when drawn in a cloverleaf structure, have anticodon specificities for proline. we have termed these isoacceptors trna1pro and trna2pro. aminoacylation studies confirmed the specificity for proline. the two forms occur in approximately equal amounts in uninfected mouse and chicken cells and in moloney l ...1979115865
sensitization of myelinated nociceptive afferents that innervate monkey hand. 1979115969
[experimental protective of fraction i of the plague microbe].the immunogenic properties of the freeze-dried preparation of fraction i tisolated from the culture of avirulent pasteurella pestis strain were studied in experiments on hamadryas baboons, guinea-pigs and white mice. the preparation was found to have a pronounced protective effect in hamadryas baboons and white mice, especially when the preparation was used in mixture with incomplete freund adjuvant. in hamadryas baboons resistance to infection was shown to correlate with hemagglutionation antib ...1979116458
spontaneous zinc deficiency in marmosets, saguinus mystax.moustached marmosets, saguinus mystax, developed alopecia, skin lesions and general debilitation approximately 6 months after being brought into our laboratory. these lesions resembled the signs of zinc deficiency in pigs, rats and squirrel monkeys. the marmosets were fed a commercial monkey diet containing 150 parts per million zinc and about 100 g of apples and oranges per week. the addition of 40 parts per million zinc to the drinking water brought about improvement in hair coat and skin cond ...1979117220
[immunochemical study of heterogenous antigens y. pestis ev similar to human red cells].franctions containing heterogenous antigens y. pestis ev similar to human red cells can be obtained by the method of immunosorption of antigens by fixed antibodies on polyacrylamide gel.1979117851
[examinations on the behaviour of grampositive and gramnegative bacteria in aqua bidest and tap-water at different initial colony counts and different temperatures (author's transl)].experiments were done about the behaviour of 5 different strains of staph. aureus, strept. faecalis, e. coli, ser. marcescens and p. aeruginosa in aqua bidest and sterile tap water at 20 degrees c, 30 degrees c and 40 degrees c. the behaviour of 3 different initial colony counts in each case was examined. colony forming units (cfu) were evaluated at the beginning and after 1, 2, 4, 6 and 24 h. cfu were reduced at staph. aureus and e. coli in aqua bidest and tap water not earlier than after 4 h, ...1979120654
[oculomotor reflex evoked by vibrassae stimulation in the cat].the oculomotor effects of vibrissae stimulation have been studied in the "encéphale isolé" non-anaesthetized cat. intracellular recording of lateral rectus motoneurones showed an excitatory projection via the ipsilateral infraorbital nerve to these motoneurones. the afferent fibers are type a alpha, recuited with a low threshold (0.2-0.5 ma) and convey information about vibrissae displacement.1979120789
[serological study of nontriturated fleas following preliminary cultivation of the plague microbe].under experimental conditions there was shown a possibility of serological study of nontriturated fleas for the presence of past. pestis. hottinger's broth with gentian-violet ((0.0003%) sodium sulfite (0.03%) and a surfactant--o (see article)-7 (3%) served as the growing medium. after the nontriturated fleas were grown for 48 hours at 37 degrees c the capsular antigen was detected with the aid of the passive hemagglutination and antibody neutralization tests. detection of the capsular antigen i ...1975123679
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