| [integrative nature of yersinia pestis virulence]. | | 1987 | 2883791 |
| polyester film as an advantageous tool for light and ultrastructural examination of single cells infected with viruses. | the use of marked transparent polyester film as a support of cell monolayer, smears or sections (cryostat or paraffin), makes possible to examine the same cell preselected in the light microscope by electron microscope (em). consequently, this technique enables to use histological or immunohistological investigations (using immunofluorescence or peroxidase (px) labelling) by light microscopy before examination in em. as example we used herpes simplex virus (hsv) type 1-infected cells grown on th ... | 1987 | 2883863 |
| a new subtype ("brezová") of tribec orbivirus (kemerovo group) isolated from ixodes ricinus males in czechoslovakia. | | 1987 | 2883865 |
| novel approaches towards characterization of the high-affinity nucleotide binding sites on mitochondrial f1-atpase by the fluorescence probes 3'-o-(1-naphthoyl)adenosine di- and triphosphate. | the fluorescence properties of 3'-o-(1-naphthoyl)adenosine di- and triphosphates (termed n-adp and n-atp, respectively) were investigated in detail. of special importance for the use of these analogues as environmental probes is their high quantum yield (0.58 in water) and the polarity dependence of shape and wavelength position of the emission spectrum. upon binding of n-adp and n-atp to mitochondrial f1-atpase, the fluorescence intensity is markedly decreased, due to polarity changes and 'grou ... | 1987 | 2883993 |
| cytoplasmic calcium elevation in hippocampal granule cell induced by perforant path stimulation and l-glutamate application. | calcium-dependent fluorescence of a ca2+ indicator (fura-2) loaded in the slice of guinea pig hippocampus was measured by a microscope/video-camera/photometry system. tetanic stimulation of the perforant path (pp) or application of l-glutamate caused increment of the fluorescence from the dendritic and somatic layers of the granule cells in the dentate gyrus. magnitude of the increment depended on the frequency and intensity of the pp-stimulation or on the dose of l-glutamate. 2-aminophosphonova ... | 1987 | 2884013 |
| two distinct mechanisms for regulation of gamma-glutamyl transpeptidase in cultured rat hepatocytes by glucocorticoid-like steroids. | adult rat hepatocytes maintained in primary monolayer culture with defined medium were used to characterise two effects of glucocorticoid-like steroids in regulating gamma-glutamyltranspeptidase (ggt). low concentrations of glucocorticoids alone had little effect on ggt but synergistically enhanced induction of the enzyme by liver tumor-promoting xenobiotics such as 1,1,1-trichloro-2,2-bis-(4-chlorophenyl)-ethane (ddt) and hexachlorocyclohexane. the enhancing effect appears to be mediated by the ... | 1987 | 2885003 |
| [the spasmolytic activity of propiverine and some of its structural analogs]. | the spasmolytic activity of propiverine and its corresponding methyl ether analogue was investigated in the isolated ileum and urinary bladder of the rat. it could be demonstrated that the antispasmodic properties of propiverine were, in contrast to its methyl ether analogue, not only based on a blockade of muscarinic receptors but were also a consequence of nonspecific papaverine-like effects. investigations with three putative metabolites showed that the alcohol derivative o-desalkylpropiverin ... | 1987 | 2885006 |
| falciparum malaria probably acquired from infected skin-cut. | | 1987 | 2885675 |
| polymorphism of photopigments in the squirrel monkey: a sixth phenotype. | we describe here a trichromatic type of squirrel monkey that resembles old world monkeys in having two well-separated photopigments in the red-green part of the spectrum; the cones of this phenotype have peak sensitivities close to 430, 536 and 564 nm. the existence of such animals is predicted by a genetic model that postulates three alleles for a single locus on the x-chromosome of the squirrel monkey. the three alleles correspond to three different photopigments in the red-green spectral rang ... | 1987 | 2888125 |
| on the rate of f1-atpase turnover during atp hydrolysis by the single catalytic site. evidence that hydrolysis with a slow rate of product release does not occur at the alternating active site. | under conditions of molar excess of enzyme, isolated f1-atpase from beef heart mitochondria catalyses atp hydrolysis biphasically. the rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. the slow phase of atp hydrolysis is insensitive to edta. [gamma-32p]atp splitting in the slow phase cannot be chased from the enzyme during several catalytic turnovers. it follows from these results that the slow single-site hydrolysis of atp (kcat approximately 10(-4 ... | 1987 | 2888690 |
| genetic study of piliation in escherichia coli: implications for understanding microbe-host interactions at the molecular level. | | 1987 | 2892189 |
| loss of heterozygosity on chromosomes 3, 13, and 17 in small-cell carcinoma and on chromosome 3 in adenocarcinoma of the lung. | by a molecular genetic approach using polymorphic dna markers that detect allelic deletion of specific chromosomal regions, we analyzed for possible loss of chromosomal heterozygosity in five different histological types of lung cancers obtained from 47 patients. in small-cell carcinomas, the incidence of allelic deletions at three different chromosomal loci was extremely high; loss of heterozygosity was detected on chromosomes 3p in 7 of 7 patients (100%), 13q in 10 of 11 patients (91%), and 17 ... | 1987 | 2892196 |
| [the physiological effects of beta-adrenergic receptor stimulant on the rabbit lacrimal gland]. | | 1987 | 2894752 |
| inhibitory effects of atropine and adrenergic antagonists on the changes in autonomic receptors and cyclic nucleotides of rat parotid and submandibular glands caused by sympathetic nerve stimulation. | the changes in cyclic amp (camp) concentration and density of beta-adrenoceptors caused by electrical stimulation of the sympathetic innervation to parotid and submandibular glands of rat did not occur when the alpha- and beta-adrenergic antagonists, phentolamine, and propranolol were administered 20 min prior to initiation of stimulation. they also did not occur when phentolamine, the beta-adrenergic antagonist, was administered alone prior to nerve stimulation, indicating that beta-adrenocepto ... | 1988 | 2895130 |
| sex as a risk factor for htlv-i spread among intravenous drug abusers. | | 1988 | 2895250 |
| enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta. | we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ... | 1987 | 2895427 |
| development of aids, hiv seroconversion, and potential co-factors for t4 cell loss in a cohort of intravenous drug users. | a cohort of 334 intravenous (iv) drug users from new york city drug treatment programs were followed over a mean 9-month period. among the 165 who were seropositive at enlistment, four developed clinical aids, for an annual rate of 3%. elevated iga was a significant predictor of developing aids. among 72 subjects who were initially seronegative and who were re-interviewed, four were seropositive at follow-up, for a seroconversion rate of 7% per year among seronegatives. among seropositive subjec ... | 1987 | 2896511 |
| steroid regulation of somatostatin mrna in the rat hypothalamus. | the participation of gonadal steroid hormones in the regulation of the expression of the somatostatin gene in the hypothalamus and cerebral cortex was studied by using a quantitative densitometric hybridization assay which allows the direct measurement of specific somatostatin mrna levels. the levels of somatostatin mrna in hypothalamus were found to be significantly decreased following gonadectomy in both male and female rats (67% in males and 75% in females). moreover, with in situ hybridizati ... | 1988 | 2897361 |
| the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains. | proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ... | 1988 | 2897367 |
| analysis of esmolol in human blood by high-performance liquid chromatography and its application to pharmacokinetic studies. | | 1988 | 2897375 |
| the effect of selective electrical stimulation of non-myelinated vagal fibres on heart rate in the rabbit. | the bradycardia evoked by electrical stimulation of the peripheral cut end of the rabbit vagus nerve is mediated by both myelinated and non-myelinated fibres. the purpose of this study was to assess the effects of non-myelinated fibres on heart rate in the rabbit using selective electrical stimulation techniques. in 8 rabbits selective activation of non-myelinated fibres using reversed polarity triangular shaped pulses (10 hz, 20 s), resulted in a slowly developing fall in heart rate of 24.1 +/- ... | 1987 | 2897392 |
| regional levels of neurotransmitter markers in the pigeon telencephalon: a comparison with possibly homologous areas of the rat telencephalon. | the levels of cholinergic, gamma-aminobutyric acidergic (gabaergic), and excitatory amino acid neurotransmitter markers have been measured in 18 regions of the pigeon telencephalon as well as in supposedly homologous areas of the rat telencephalon. among the basal telencephalic areas, some similar patterns of regional distribution were observed, with the noticeable exception of the ratio of levels of cholinergic markers between the striatum and globus pallidus, which was much larger in the rat t ... | 1988 | 2897425 |
| dextrorphan and dextromethorphan, common antitussives, are antiepileptic and antagonize n-methyl-d-aspartate in brain slices. | the antitussive, dextromethorphan (dm), and its metabolite, dextrorphan (dx), were evaluated for antiepileptic properties in vitro. interictal bursts and prolonged ictal epileptiform afterdischarges, induced by perfusion of guinea pig neocortical brain slices with mg2+-free solution, were blocked by dx (1-250 microm) or dm (100 microm). intracellular records showed that these agents blocked n-methyl-d-aspartate (nmda)-induced depolarizations without altering intrinsic membrane properties. dx blo ... | 1988 | 2897648 |
| the prevention and avoidance of genetic disease: summing up. | the preceding papers have dealt with major advances in understanding and detecting the mutational basis of human disease. if these advances are to be of practical benefit, systems of effective, efficient and acceptable delivery of the technology to the relevant population groups will need to be planned. in these delivery systems, the key figure is likely to be the clinical geneticist, still a somewhat shadowy figure, difficult to define: a doctor among scientists, and a scientist among doctors. ... | 1988 | 2900529 |
| quantitation of pancuronium, 3-desacetylpancuronium, vecuronium, 3-desacetylvecuronium, pipecuronium and 3-desacetylpipecuronium in biological fluids by capillary gas chromatography using nitrogen-sensitive detection. | a sensitive and specific capillary gas chromatographic (gc) assay was developed for the quantitation of the quaternary ammonium steroidal neuromuscular blocking drugs pancuronium (panc), vecuronium (vec) and pipecuronium (pip), as well as the metabolites 3-desacetylpancuronium (3-despanc) and 3-desacetylvecuronium (3-des vec) in plasma, bile and urine; the putative metabolite 3-desacetylpipecuronium (3-des pip) was extracted and quantitated only in urine. the procedure employed a single dichloro ... | 1988 | 2900840 |
| atp-dependent glutamate uptake into synaptic vesicles from cerebellar mutant mice. | the atp-dependent glutamate uptake system in synaptic vesicles prepared from mouse cerebellum was characterized, and the levels of glutamate uptake were investigated in the cerebellar mutant mice, staggerer and weaver, whose main defect is the loss of cerebellar granule cells, and the nervous mutant, whose main defect is the loss of purkinje cells. the atp-dependent glutamate uptake is stimulated by low concentrations of chloride, is insensitive to aspartate, and is inhibited by agents known to ... | 1988 | 2901460 |
| responses of hippocampal pyramidal cells to putative serotonin 5-ht1a and 5-ht1b agonists: a comparative study with dorsal raphe neurons. | in low cerveau isolé transected rats, the effects of microiontophoretic application of putative serotonin 5-ht1a and 5-ht1b agonists on the spontaneous firing rate of ca1 pyramidal cells were compared to those of 5-ht. in contrast to the large current-dependent suppression of unit activity observed with 5-ht, the 5-ht1a compounds, ipsapirone, 8-oh-dpat (8-hydroxy-2-(di-n-propylamino)-tetralin) and ly 165163 (p-aminophenylethyl-m-trifluoromethylphenylpiperazine) and the 5-ht1b compounds, mcpp (m- ... | 1988 | 2901680 |
| measurement of dynorphins with 3h-etorphine and canine limbic system receptors. | a canine limbic system preparation was used as the source of opioid peptide receptors to screen biologic extracts for the presence of opioid receptoractive peptides following their gradient rp-hplc separation. eight synthetic dynorphin peptides were studied for their ability to displace the commonly-used ligand 3h-etorphine from the canine limbic system p2 preparation. the peptides studied included the dynorphins 1-7, 1-8, 1-9, 1-10, 1-12, 1-13, 1-17, and dynorphin b. two different types of opio ... | 1988 | 2901686 |
| clinical and serological features of human herpesvirus-6 infection in three adults. | 3 adult patients with serological evidence of human herpesvirus-6 (hhv-6) infection had mild, afebrile illnesses with nonspecific symptoms. in each case, the characteristic clinical feature was the presence of enlarged, bilateral, non-tender, anterior and posterior cervical nodes early in the illness which persisted for up to 3 months. hhv-6 igg antibody reciprocal titres of 160 to 2560 were found during acute infection, and decreased to a titre of 10 in 1 patient 3 years later. igm responses we ... | 1988 | 2902266 |
| c-erba protooncogenes mediate thyroid hormone-dependent and independent regulation of the rat growth hormone and prolactin genes. | regulation of gene expression by the thyroid hormones is thought to be mediated by a nuclear-associated receptor found in a wide variety of cells and tissues. cellular homologues of the avian erythroblastosis virus oncogene, v-erba, encode proteins which bind thyroid hormone with similar affinities as thyroid hormone receptors. however, it has not been shown that any of the c-erba proteins can function as receptor and modulate thyroid hormone responsive genes. in this study, using transient expr ... | 1988 | 2903439 |
| foot trauma due to rodents in sierra leone. | | 1988 | 2904187 |
| [pili (fimbriae) of the causative agents of plague and pseudotuberculosis: their detection in the hemagglutination reaction, the passive hemagglutination reaction and in an immunoenzyme analysis system]. | newly developed serological methods for the detection of pili in the passive hemagglutination (pha) test with the use of immunoglobulin erythrocytic diagnosticum and in the enzyme immunoassay (eia) with the use of specific immunoglobulins labeled with horse radish peroxidase have been found to exceed the method of detecting pili, based on the determination of their hemagglutinating activity, in sensitivity and specificity. besides, the pha test and eia have proved to be capable of detecting low ... | 1988 | 2904200 |
| ca2+-dependent and ca2+-independent glutamate release, energy status and cytosolic free ca2+ concentration in isolated nerve terminals following metabolic inhibition: possible relevance to hypoglycaemia and anoxia. | hypoglycaemia and anoxia both cause massive release of glutamate from the brain in vivo, and the nature of this release was investigated using guinea-pig cerebral-cortical synaptosomes and iodoacetate and rotenone to simulate the energetic consequences of these conditions. glutamate release (by continuous fluorimetry), cytoplasmic free ca2+ (by fura-2), membrane potentials, atp, adp and creatine phosphate were determined in parallel, following the addition of iodoacetate or rotenone, alone or in ... | 1988 | 2904664 |
| [effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs]. | effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ... | 1988 | 2906315 |
| regional localization of the autosomal dominant polycystic kidney disease locus. | the localization of the autosomal dominant polycystic kidney disease locus (pkd1) within an array of anonymous polymorphic dna sequences on chromosome 16 band p13 was determined by multipoint mapping. nine polymorphic dna markers, including two hypervariable sequences, were used to study 19 pkd1 and 21 reference families. pkd1 was found to lie proximal to the 3' and 5' hypervariable regions of alpha-globin and distal to the anonymous sequence cri-0327. somatic cell hybrid mapping places pkd1 wit ... | 1988 | 2906325 |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| changes in the metabolic profile of the equine gluteus medius as a function of sampling depth. | 1. cross sections from the middle of the gluteus medius were removed from 10 adult horses and used to evaluate changes in histochemically determined muscle fiber type and biochemically determined metabolic enzyme activities as a function of sample depth. 2. muscle fiber types determined using histochemical methods for myosin atpase (ph 9.4) and succinic dehydrogenase (sdh) activity indicated percent fast-twitch glycolytic (fg) muscle fibers decreased and slow-twitch oxidative (so) fibers increas ... | 1988 | 2907449 |
| isolation and partial characterization of rhizolysin, a high molecular weight protein with hemolytic activity, from the jellyfish rhizostoma pulmo. | a new cytolysin has been isolated from the nematocysts of the jellyfish, rhizostoma pulmo, and named rhizolysin. the hemolysin has a mol. wt of approximately 260,000, a sedimentation coefficient of 10.3 s and is rod-shaped with a calculated axial ratio of about 1:5. it appears to be composed of three subunits with a pi value near 7.8. rhizolysin shows no phospholipase a activity, nor an induction period for its hemolytic activity and is completely inhibited by sucrose. the optimum ph was 6.75. t ... | 1988 | 2907686 |
| [double control of pili formation in yersinia pestis]. | | 1988 | 2907926 |
| neuropeptide-dopamine interactions. iii. cyclo(his-pro) and persistence of dopaminergic supersensitivity on withdrawal from one year continuous neuroleptic treatment. | when rats are given dopaminergic antagonists (neuroleptics) continuously for several days behavioral supersensitivity to dopaminergic agonists results. this supersensitivity, however, declines rapidly on neuroleptic withdrawal. the data presented here, however, are the first to show that the supersensitivity persists for a longer duration (greater than 100 days) if the neuroleptic is administered continuously for 1 year. oral administration of 0.76 +/- 0.13 mg/kg/day cyclo(his-pro), a peptide ex ... | 1988 | 2908706 |
| biosynthesis of proteochondroitin sulfate by hl-60 human promyelocytic cells. | human promyelocytic cells (hl-60) were labeled with 35s-sulfate and either 3h-glucosamine or 3h-serine as precursors. accumulation of 35s-labeled macromolecules was approximately linear for up to 96 h, with a mean cell:medium ratio of 5.5:1, although activity/10(5) viable cells reached a plateau level after 24 h. virtually none of the cell-associated proteoglycan was removed by trypsinization, consistent with a predominantly intracellular localization. proteoglycan heterogeneity was investigated ... | 1989 | 2911020 |
| polyclonal anti-idiotypes influence macrophage chemotaxis in coxsackievirus-induced murine myocarditis. | investigation into the mechanism(s) whereby monocytes-macrophages are mobilized to the myocardium of coxsackievirus (cvb3)-infected mice was approached by assessing the role of anti-idiotypic antibodies against specific antiviral igg molecules. balb/c mice preinoculated with polyclonal anti-idiotypic, syngeneic antibodies resulted in significantly decreased myocarditis after virus challenge. on the other hand, oil-induced peritoneal exudate cells obtained from virus-exposed animals demonstrated ... | 1989 | 2911021 |
| arthrodesis of metatarsophalangeal joints in a dog. | chronic osteomyelitis and septic arthritis involving the 3rd and 4th metatarsophalangeal joints were diagnosed in a doberman pinscher. arthrodesis of the 3rd and 4th metatarsophalangeal joints resulted in a subtle persistent gait abnormality, but limb function was preserved. arthrodesis of metatarsophalangeal joints is an alternative to amputation, especially when main weight-bearing digits are involved. | 1989 | 2914800 |
| serologic evidence of yersinia pestis infection in small mammals and bears from a temperate rainforest of north coastal california. | from 1983 to 1985, 463 serum samples from 11 species of mammals in redwood national park (rnp) (california, usa) were evaluated for antibodies to yersinia pestis by the passive hemagglutination method. yersinia pestis antibodies occurred in serum samples from 25 (36%) of 69 black bears (ursus americanus), one (50%) of two raccoons (procyon lotor), five (3%) of 170 dusky-footed woodrats (neotoma fuscipes), and one (less than 1%) of 118 deer mice (peromyscus maniculatus). two hundred seventy-three ... | 1989 | 2915403 |
| potassium secretion by the cortical collecting tubule: effect of c1 gradients and ouabain. | in various epithelia k and cl transport are molecularly coupled and kcl cotransport is dependent on the na-k pump. the present study examines 1) the effect of a bath-to-lumen cl gradient on k secretion during active na transport and 2) the effect of basolateral ouabain on k secretion and na absorption in the presence and the absence of a bath-to-lumen cl gradient. under symmetrical conditions there was significant k secretion (jk = -24.0 +/- 3.9 pmol.mm-1.min-1) and cl secretion (jcl = -15.7 +/- ... | 1989 | 2916662 |
| molecular cloning and analysis of full-length cdnas cognate to a rat gene under multihormonal control. | gene 33 is a multihormonally regulated rat gene whose transcription is rapidly and markedly enhanced by glucocorticoids, insulin, or camp. a cdna clone (p216) containing a nearly full-length dna complementary to the mrna of this gene was isolated from a cdna library and sequenced. the cdna represents the entire mrna transcript, except for three bases at the 5' terminus. the message is 2970 nucleotides long and can encode a protein of 459 amino acids with a molecular mass of 49,919 da. the deduce ... | 1989 | 2916834 |
| determinants of vo2max in rats after high-intensity sprint training. | the hemodynamic response to maximal exercise was determined in rats that were subjected to high-intensity sprint training (hist) and rats that served as sedentary controls. training consisted of five 1-min bouts of treadmill running at work loads (15% grade, 97 m/min) in excess of the animals' maximal o2 uptake (vo2max) interspersed with 90 s of rest. training was performed 6 days/wk for 6 wk. after the training regimen, all rats were acutely instrumented with catheters in the right carotid arte ... | 1989 | 2917921 |
| structure-activity relationships of pyrimidine nucleosides as antiviral agents for human immunodeficiency virus type 1 in peripheral blood mononuclear cells. | the structure-activity relationships of several pyrimidine nucleosides related to 3'-azido-3'-deoxythymidine (azt) were determined in human immunodeficiency virus type 1 (hiv-1) infected human peripheral blood mononuclear cells. these studies indicated that nucleosides with a 3'-azido group on the sugar ring exhibited the most potent antiviral activity. substitution at c-5 with h, ch3, and c2h5 produced derivatives with the highest potency, whereas alkyl functions greater than c2, including brom ... | 1989 | 2918508 |
| effect of ivermectin on the ear mange mite, psoroptes cuniculi, of rabbits. | rabbits, naturally infected with ear mange mite, psoroptes cuniculi, were injected subcutaneously with a single dose of ivermectin at 200 micrograms or 400 micrograms/kg of body weight. the effect of the drug was evaluated clinically and parasitologically over 4 weeks. the animals in both groups became negative for mites 6 days after treatment and remained so until the end of trial. the ear lesions disappeared in both groups. however, the regression of lesions was faster in rabbits administered ... | 1989 | 2920276 |
| nerve terminal morphology in c57bl/6nnia mice at different ages. | the mammalian neuromuscular junction (nmj) appears to undergo a dynamic remodeling process with advancing age. previous studies suggest that the nmj becomes progressively larger with age, to a greater degree in fast-twitch muscles than slow-twitch muscles. to better understand this process, quantitative morphometry was conducted on soleus and extensor digitorum longus (edl) nerve terminals from c57bl/6nnia mice aged 4, 8, 10, 12, 18, 22, 24, and 32 months. computer-aided morphometry of camera lu ... | 1989 | 2921469 |
| liver sinusoidal blood containing natural killer-like cells. | rat liver sinusoidal washout cells were examined. these cells, which are marginated in sinusoids, could be washed out by simple flushing of the vasculature with culture media without enzymes and under physiological portal pressure. they revealed, in comparison to peripheral blood mononuclear cells, high cytotoxic activity commonly attributed to the natural killer (nk) and natural cytotoxic (nc) cells, and were found to be anti-asialo-gm1-negative. liver sinusoidal cytotoxic cell (lscc) activity ... | 1989 | 2922574 |
| sulfate transport in human lung fibroblasts (imr-90): effect of ph and anions. | we previously reported the presence of a carrier-mediated sulfate transport system in human lung fibroblasts (imr-90) (a. elgavish, j. b. smith, d. j. pillion, and e. meezan. j. cell. physiol. 125: 243-250, 1985). kinetic studies carried out in the lung fibroblasts show that cl- inhibits so4(2-) uptake in a competitive manner. taken together with the fact that high extracellular cl- stimulates so4(2-) efflux, these results suggest that so4(2-) uptake into lung fibroblasts occurs via a so4(2-)-cl ... | 1989 | 2923189 |
| differential clearance and host-pathogen interactions of yope- and yopk- yopl- yersinia pestis in balb/c mice. | this study characterized infections in balb/c mice by the nonpigmented yersinia pestis kim and its derivatives lacking the low-ca2+-response virulence plasmid pcd1 or failing to express selected yersinial outer membrane proteins (yops). the parent y. pestis showed net growth in the spleen by 2 h and in the liver after 7 h; exponential growth in both the liver and spleen culminated in death of the mice starting on day 4, with total bacterial numbers of less than 10(8) in the blood, liver, and spl ... | 1989 | 2925246 |
| britain launches genome program. | | 1989 | 2928800 |
| immunocytochemical demonstration of langerhans' cells in smears and pellets of exfoliated cells from human exocervices. | immunocytochemical staining with okt6 monoclonal antibody and s-100 protein antiserum was used to reveal langerhans' cells in smears and/or pellets of exfoliated cells from uterine cervices (normal and with squamous carcinoma). immunoreactive langerhans' cells were found exclusively in smears and pellets of cervices with squamous carcinoma. langerhans' cells, which appear as rounded cells, showed a peripheral ring of intense fluorescence with okt6 antibody whereas the entire cell stained with s- ... | 1989 | 2929224 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| [influence of pyronaridine phosphate on three-generation reproduction in rats]. | | 1985 | 2934948 |
| [results of a trial of a plague monoclonal antibody erythrocyte diagnostic agent]. | plague antibody monoclonal erythrocyte diagnosticum was studied in serological tests simultaneously with commercial plague antibody erythrocyte diagnosticum prepared on the basis of hyperimmune horse serum and with commercial plague antigenic erythrocyte diagnosticum. in this investigation the suspensions of numerous strains of yersinia pestis, other closely related and heterologous organisms, experimentally infected wild and laboratory animals, as well as samples of materials obtained from smal ... | 1985 | 2936047 |
| radioimmunoassay of atrial natriuretic peptides in human plasma. | the concentration of atrial natriuretic peptide (hanp) in plasma from venous blood of healthy subjects was measured by radioimmunoassay. hanp from 5 ml of edta-treated plasma was adsorbed onto sep-pak c18 cartridges, which were eluted with methanol/trifluoroacetic acid (5 ml/l), 90/10 by volume. the eluates were concentrated by evaporation under nitrogen and lyophilized. after redissolving the samples in 0.5 ml of sodium phosphate buffer, we incubated 100-microl aliquots with anti-alpha-hanp for ... | 1986 | 2936535 |
| [emergency cardiac pacing via a coronary vessel during percutaneous coronary angioplasty]. | percutaneous coronary angioplasty (pca) is sometimes complicated by bradyarrhythmias necessitating emergency temporary cardiac pacing. this is usually performed by the classical transvenous endocardial approach. this experimental study investigated the possibility of using the metallic guide wire used during pca as a monopolar electrode. systematic electrical stimulation at different levels of the coronary arteries in 6 anaesthetised pigs showed threshold levels in the distal segments of 3 to 15 ... | 1985 | 2937382 |
| [growth of different types of muscle fibers of the soleus muscle during postnatal ontogeny in the rat]. | in order to study the development of the m. soleus muscle fibers during postnatal ontogenesis in the rat, methods for revealing atpase activity of myosin at preincubation in acidic and alcaline medium and lactate dehydrogenase and succinate dehydrogenase activity have been used. the m. soleus undergoes three stages of development. the first stage--from birth of the animal up to the 7th day. during this time the muscle is homogenous. the second stage is characterized by appearance of certain hist ... | 1986 | 2937393 |
| comparative study of coronary vasodilator and cardiac effects of nitrendipine by use of isolated, blood-perfused dog heart preparations. | the coronary vasodilator and cardiac effects of 3-ethyl-5-methyl-1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl) -3,5-pyridinedicarboxylate (nitrendipine, bay e 5009) were compared in isolated, blood-perfused sinoatrial (sa) node, atrioventricular (av) node and papillary muscle preparations of dogs with i.a. administration. in all preparations nitrendipine increased (coronary) blood flow. in sa node preparations nitrendipine reduced sinus rate but the reduction remained only about 13% of the basal va ... | 1986 | 2937413 |
| chromophore/protein interaction in bacterial sensory rhodopsin and bacteriorhodopsin. | retinal analogues with altered conjugated double bond systems or altered stereochemistry were incorporated into the phototaxis receptor sensory rhodopsin (sr) and the light-driven proton pump bacteriorhodopsin (br) from halobacterium halobium. wavelength shifts in absorption ("opsin shifts") due to analogue interaction with the protein microenvironment demonstrate that the same overall electrostatic and steric properties of the retinal binding-site structures exist in both proteins despite their ... | 1986 | 2937462 |
| cultured human endothelial cells synthesize a plasma membrane protein complex immunologically related to the platelet glycoprotein iib/iiia complex. | we have previously demonstrated that endothelial cells synthesize a plasma membrane protein indistinguishable from platelet glycoprotein (gp) iia. the present study provides evidence for a further analogy between the platelet and the endothelial cell membrane by showing that cultured endothelial cells also synthesize a membrane protein complex immunologically related to the platelet gp iib/gp iiia complex. this evidence is based on the following observations: (1) c17, a murine monoclonal antipla ... | 1986 | 2937471 |
| complement, membrane glycoproteins, and complement receptors: their role in regulation of the immune response. | to determine the effect of complement on the normal antibody response we studied seven patients with genetically determined complement component deficiencies, guinea pigs deficient of c4 and c2, respectively, and two patients whose neutrophils and monocytes lack the c3bi receptor. patients deficient of early complement components (c4, c2, c3) have abnormal antibody responses to the t-cell-dependent antigen, bacteriophage phi x 174. complement-deficient guinea pigs (c4, c2) produce less antibody ... | 1986 | 2941194 |
| dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility. | in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ... | 1986 | 2942039 |
| the (ca2+ + mg2+)-stimulated atpase of the rat parotid endoplasmic reticulum. | a membrane fraction enriched in endoplasmic reticulum was prepared from rat parotid glands by using sucrose-gradient centrifugation. the fraction showed a 10-fold increase in specific activity of nadph: cytochrome c reductase activity over that of tissue homogenates and minimal contamination with plasma membranes or mitochondria. the endoplasmic reticulum fraction possessed both mg2+ -stimulated atpase as well as ca2+, mg2+-atpase [( ca2+ + mg2+)-stimulated atpase]activity. the ca2+, mg2+-atpase ... | 1986 | 2943271 |
| termination and reinitiation signals of bacteriophage phi x174 rolling circle dna replication. | the nucleotide sequence requirements for termination and reinitiation of rolling circle dna replication within the 30-bp phi x174 origin region were studied. plasmids were constructed which contained a complete and a partial phi x174 origin region in the same orientation. the partial origin consisted of the first 16, 24, 25, 26, 27, or 28 bp of the origin region. plasmids harboring a complete origin region are subject to rolling circle dna replication and packaging of single-stranded plasmid dna ... | 1986 | 2945311 |
| interaction studies in mice of sch 29851, a potential non-sedating antihistamine, with commonly used therapeutic agents. | sch 29851 was studied for its ability to interact with selected drugs used in man. the non-sedating antihistamine terfenadine and the sedating antihistamine diphenhydramine were also studied for comparison. sch 29851 at 80 mg/kg po in mice, a dose about 50 times its ed50 for blocking histamine-induced paw edema in the same species, potentiated the anticonvulsant effects of diazepam. at the high dose of 320 mg/kg po, about 80 times its ed50 for antihistamine effects, sch 29851 potentiated the abi ... | 1986 | 2945410 |
| a comparison of five different methods for the detection of tnp specific mouse ige: elisa, elisa on cells, rosetting, granule enzyme release assay and passive cutaneous anaphylaxis. | using the same anti-tnp hybridoma supernatant pool as ige antibody source (2 micrograms/ml), several methods were compared for their sensitivity in ige detection and convenience for screening purposes. using rat basophilic leukemia (rbl) cells as specific receptor cells, one out of two rosetting methods with tnp-sheep red blood cells allowed the detection of 0.5 ng ige/ml (50 pg/assay) while the other was much less sensitive (60 ng/ml). more convenient for screening was an elisa method performed ... | 1986 | 2945867 |
| effect of mononuclear cell factors on calcium transport in sarcoplasmic reticulum vesicles. | the effect of supernatants from cultures of mitogen-stimulated human mononuclear cells on calcium transport by sarcoplasmic reticulum was examined. calcium transport was assayed by measuring the time course of calcium accumulation by sarcoplasmic reticulum incubated with supernatants from stimulated mononuclear cells was 20% less than that by vesicles exposed to control supernatants (p less than 0.001). in contrast, no difference in calcium-dependent atpase activity was noted between vesicles in ... | 1986 | 2946307 |
| tight genetic linkage of a glucosyltransferase and dextranase of streptococcus mutans gs-5. | a genetic library consisting of over 5000 clones with an average insert size of 6.9 kilobasepairs (kbp) of streptococcus mutans gs-5 has been constructed in a bivalent plasmid vector pmk3, which is capable of replicating in escherichia coli and bacillus subtilis. the recombinant plasmid psucri, containing a 6.0 kbp fragment of s. mutans gs-5 dna, was the focus of this study. using southern hybridization, in vitro and in vivo gene expression techniques, and biochemical analysis, this clone was sh ... | 1986 | 2946734 |
| [interaction of yersinia pestis bacteria with bacteriophage mu]. | yersinia pestis cells are shown to be sensitive to bacteriophage mu cts62 infection. lysis of bacteria has been shown to be more efficient on solid nutrient medium than in lb broth. 10(-5) pfu per ml is the maximal concentration of bacteriophage particles yielded from the broth cultures of bacteria. moi 0.1 has been used to obtain such yields of bacteriophage. lysogenization of yersinia pestis cells has not been achieved when the standard methods of bacteriophage infection have been used. it was ... | 1985 | 2948125 |
| influence of intravenous and intracerebroventricular vasopressin on baroreflex control of renal nerve traffic. | we performed experiments in alpha-chloralose-anesthetized rabbits with vagi sectioned, to determine the influence of intravenous and intracerebroventricular vasopressin on arterial baroreflex control of renal nerve activity. arterial baroreflex control of renal nerve activity was assessed during phenylephrine-induced increases and nitroglycerin-induced decreases in arterial pressure. intravenous vasopressin (4 and 40 mu over 1 minute) reduced basal renal nerve activity (from 149 +/- 14 to 101 +/ ... | 1986 | 2935322 |
| studies on the atp-sensitivity of yeast phosphofructokinase by means of affinity partitioning using polymer bound cibacron blue f3g-a. | yeast phosphofructokinase was partitioned in an aqueous two-phase system composed of polyethylene glycol and dextran in which a small amount of the first polymer was replaced by cibacron blue f3g-a substituted polyethylene glycol. it was found that the partition coefficient, k, of the enzyme determined immediately after adding the enzyme to the system and within a series of time intervals (min) shifts from a lower to a higher value. this effect was amplified when the enzyme was preincubated with ... | 1985 | 2935143 |
| neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine. | an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ... | 1987 | 2951610 |
| low molecular mass inhibitors from calf thymus selective for t-lymphocyte proliferation. | endogenous factors preferentially inhibiting t-lymphocyte proliferation were prepared from the acetone precipitate of a 60% ethanol extract from calf thymus and their biochemical properties examined. by ultrafiltration the strongest lymphocyte-selective inhibition was found in the molecular mass range between 1 and 5 kda. fast protein liquid chromatography (fplc) of this fraction on an anion exchange column eluted the lymphocyte inhibitors at 205-265 mm ammonium acetate. staining procedures foll ... | 1986 | 2953126 |
| workers' education. | | 1987 | 2953594 |
| workers' education. | | 1987 | 2953594 |
| effect of live and heat-killed bacteria on the secretory activity of paneth cells in germ-free mice. | germ-free mice were given live or heat-killed facultative anaerobes, and the ultrastructure of ileal paneth cells was quantitatively examined with special reference to secretory granules showing a bipartite substructure (central core and peripheral halo). after administering live or heat-killed bacteria, there was a decrease in the area occupied by the cores of secretory granules in paneth cells, and exocytosed core material was observed in the crypt lumen. there were no changes in the area occu ... | 1988 | 2963697 |
| systolic and diastolic dysfunction during atrial pacing in conscious dogs with left ventricular hypertrophy. | to determine the extent to which the hypertrophied left ventricle responds to the chronotropic stress induced by graded atrial pacing rates, we studied conscious, chronically instrumented dogs with severe compensated pressure overload left ventricular (lv) hypertrophy induced by aortic banding in puppies 8-10 weeks of age. at 1-2 years, dogs with severe lv hypertrophy (lv free wall/body wt ratio 6.8 +/- 0.6 g/kg) and sham-operated littermates (lv free wall/body wt ratio 4.0 +/- 0.3 g/kg) were in ... | 1988 | 2963705 |
| the effects of selective dopamine receptor agonists and antagonists on body temperature in rats. | body temperature was measured at repeated time intervals following the administration of various dopamine agonists and antagonists. the d-1 and d-2 receptor agonist, apomorphine, produced dose-dependent hypothermia. this effect was inhibited by the d-2 receptor antagonist, spiroperidol. stimulation of d-2 receptor by ly171555 produced dose-dependent hypothermia, which was attenuated by pretreatment with spiroperidol and not altered by the d-1 receptor antagonist sch23390. the d-1 receptor agonis ... | 1987 | 2951265 |
| [orientational behavior of the ixodid tick hyalomma asiaticum under desert conditions]. | the orientational behaviour of the imago of ticks in natural conditions is based on the optimal using of relatively primitive eyes with the development of a specific mechanism of orientation. a statistical study of tracks of ticks together with the analysis of the optical system and functional possibilities of the eyes have made it possible for the author to suggest a model of orientational mechanism based on different signal meanings of increasing and decreasing of light intensity registered by ... | 1986 | 2950366 |
| helper t-cell clones that recognize autologous insulin are stimulated in nonresponder mice by pork insulin. | murine antibody responses to various species of insulin are under major histocompatibility complex-linked ir gene control. beef insulin differs from pork insulin by only two amino acids in the a-chain loop, yet strain c57bl/10 (b10) mice produce insulin-specific antibodies after immunization with beef insulin and fail to produce antibody after stimulation with pork insulin. nevertheless, pork insulin primes helper t cells in b10 mice that can be demonstrated if insulin-specific lyt-1-, -2+ suppr ... | 1988 | 2965814 |
| [antigenuria in plague-infected great gerbils]. | in the urine of plague-infected great gerbils yersinia pestis capsular antigen was detected by means of diagnostic preparations, both commercial and experimental (based on monoclonal antibodies). the antigen was detected in many urine samples taken from the animals over a prolonged period. the incidence and duration of antigenuria were found to be related to the survival time of great gerbils after infection and the level of antibodies in their blood. the number of animals with antigenuria marke ... | 1988 | 2966529 |
| homology of glucosyltransferase gene and protein sequences from streptococcus sobrinus and streptococcus mutans. | the sequences of glucosyltransferase genes from streptococcus sobrinus (gtfi) and streptococcus mutans (gftb) were compared and show a high degree of homology. there is a 57.7% homology of nucleotides in the genes and a 56.7% homology of amino acids in the deduced protein sequences. the g + c content for the protein-coding region is 43.6% for s. sobrinus and 41.2% for s. mutans. internal repeating sequences present in both proteins exhibit some difference in sequence pattern. | 1988 | 2971696 |
| [antagonistic and insecticidal properties of soil micromycetes]. | it was shown that microscopic fungi isolated in plague foci had an antagonistic effect on the plague causative agent. in the majority of the cases it was associated with their insecticidal activity against plague-transmitting fleas. it is expedient to consider the relations revealed between the burrow biocenosis components in investigation of plague enzootic aspects and development of new biological insecticides for control of the infection carriers. | 1988 | 2973774 |
| transcriptional mapping and nucleotide sequence of the escherichia coli fepa-fes enterobactin region. identification of a unique iron-regulated bidirectional promoter. | the iron-controlled fepa and fes-entf transcripts from the escherichia coli enterobactin gene complex are expressed divergently from a limited genetic region, thereby suggesting the existence of a single, possibly overlapping promoter junction for these genes. the nucleotide sequence of a 1,997-base pair hpai fragment specific for this genetic region allowed for the identification of an 1,122-base pair open reading frame as the previously uncharacterized fes gene. its product, fes (approximately ... | 1988 | 2974033 |
| the relationships between oligomeric structure and function of band 3 protein from human erythrocyte membranes: present knowledge and suggestions for further experiments. | | 1988 | 2976450 |
| cosmid cloning of rickettsia prowazekii antigens in escherichia coli k-12. | rickettsia prowazekii dna was partially digested with sau3a or hindiii, ligated with the cosmid vector phc79, packaged in vitro, and transduced into escherichia coli hb101. cosmid cloning of sau3a-digested rickettsial dna yielded 1,288 ampicillin-resistant colonies; 798 cosmid clones resulted with hindiii-digested rickettsial dna. chimeric cosmid dna was extracted from the latter gene bank, digested to completion with hindiii, and compared by agarose gel electrophoresis with a hindiii digest of ... | 1985 | 2981194 |
| corticotropin-releasing factor desensitization of adrenocorticotropic hormone release is augmented by arginine vasopressin. | the desensitization of corticotropin-releasing factor (crf)-stimulated acth release from and intracellular cyclic amp accumulation in anterior pituitary cells was investigated in primary cultures of rat pituitary cells and in a mouse tumor cell line (att 20/d16-16). crf potently stimulated acth secretion and cyclic amp accumulation in both preparations. when primary cultures were preincubated with 100 nm crf, 50% desensitization of acth release occurred within 4 hr while similar desensitization ... | 1985 | 2981299 |
| viremia is present in incubation period in nonimmunocompromised children with varicella. | | 1985 | 2981309 |
| uridine 5'-diphosphate glucose analogues. inhibitors of protein glycosylation that show antiviral activity. | a series of analogues of uridine 5'-diphosphate glucose and uridine 5'-diphosphate glucosamine have been synthesized by reaction of 2,3,4,6-tetra-o-benzyl-, 2,3,4,6-tetra-o-benzoyl-, 2,3,4,6-tetra-o-acetyl-, and 2,3,4,6-tetra-o-palmitoyl-alpha-d-glucopyranose and 2-acetamido-3,4,6-tri-o-acetyl-2-deoxy-alpha-d-glucopyranose with chlorosulfonyl isocyanate and 2',3'-o-isopropylideneuridine. isopropylidene and acetyl groups of the resulting 5'-o-[[[[(alpha-d-glucopyranosyl)oxy]carbonyl]amino]sulfony ... | 1985 | 2981323 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| electron microscopic assessment of cervical punch biopsies in women followed-up for human papillomavirus (hpv) lesions. | cervical punch biopsies from 102 women prospectively followed-up for hpv (human papillomavirus) lesions (flat, inverted and papillomatous condylomas) were assessed on transmission electron microscopy (tem) for the presence of hpv particles. the virus discovery rate was correlated with the other characteristics of significance to the clinical behaviour (natural history) of these lesions, i.e. the colposcopic pattern, expression of papillomavirus genus specific structural antigens (demonstrated by ... | 1985 | 2988479 |
| pulsed electromagnetic fields promote collagen production in bone marrow fibroblasts via athermal mechanisms. | primary and passaged cultures of fibroblasts (rbmfs) raised from the bone marrow stroma of young rabbits were treated with pulsed electromagnetic fields (pemfs) from the start of each culture until 1 week after they became confluent. the pemf treatment had no effect on cell proliferation, estimated by phase contrast microscopy, by 3h-thymidine incorporation into dna, or by total dna assay. collagen production, estimated by conversion of 3h-proline to 3h-hydroxyproline in nondialyzable material w ... | 1985 | 2988718 |
| specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells. | the hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, gm-csf, specifically controls the production of granulocytes and macrophages. this report describes the binding of biologically-active 125i-labeled murine gm-csf to a range of hemopoietic cells. specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125i-labeled gm-csf. 125i-labeled gm-csf only appeared to bind specifically to cells in the my ... | 1985 | 2990915 |