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regional difference in airway epithelial response to neutrophil elastase: tracheal secretory cells discharge and recover in hamsters that develop bronchial secretory-cell metaplasia.human neutrophil elastase (hne) causes secretory granule discharge and conversion of many clara cells to mucous cells in hamster bronchi. we investigated whether the trachea responds to hne in a similar manner because of its abundance of clara cells. by light microscopy, the tracheal epithelium of animals exposed to a single intratracheal injection of hne was normal at 21 days, although bronchial secretory-cell metaplasia (scm) was present. an ultrastructural differential cell count showed no in ...19892612449
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...19892612450
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...19892612450
separation of iodothyronines from iodinated metabolites in serum from reverse t3 tracer kinetic studies in man and dog: a comparison of two methods.iodothyronine separation from free iodide and iodoalbumin in serial serum samples obtained from 7 human and 5 dog studies following intravenous injection of radiolabeled reverse triiodothyronine (reverse t3) was compared using acidified ammonium acetate/tetrahydrofuran (thf) elution from c-18 sep-pak cartridges or ethyl acetate/butanol (eab) extraction. both methods excluded greater than 98% free iodide and greater than 99% iodoalbumin from the iodothyronine fraction. recovery of labeled reverse ...19892613183
primary structure of a ribonuclease from bullfrog (rana catesbeiana) liver.a pyrimidine base-specific ribonuclease was purified from bullfrog (rana catesbeiana) liver by means of cm-cellulose column chromatography and affinity chromatography on heparin-sepharose cl-6b, which gave single band on sds-slab electrophoresis. the primary structure of the bullfrog liver rnase was determined. it consisted of 111 amino acid residues, including 8 half-cystine residues. from the sequence, it was concluded that three disulfide bridges in rnase a were conserved in the bullfrog rnas ...19892613682
[enhanced blocking capacity of the plague microbe in the body of the flea].during the stay of plague microbe in the organism of flea, under the effect of bactericidal factor increases its ability to form the block of proventriculus in these insects. thus, in fleas infected on white mice, which were infected in a natural way through blocked individuals, the block of proventriculus appears 2 to 3 times as often (59.5 and 34.0%) as in insects (15.02%) infected on animals, which were infected artificially.19892616196
the black death past and present. 1. plague in the 1980s.this paper considers firstly the epidemiology of plague in the 1980s. the largest number of cases occurred in tanzania. most cases were in children and young adults; in the usa the male:female ratio was about 2:1. plague had a seasonal distribution. almost all cases arose from bites of infected rodent fleas, and rattus spp. were the most important reservoir hosts. virulence is linked with the presence of a 45 mda plasmid. the predominant clinical form of plague is bubonic, followed by septicaemi ...19892617596
[genetic analysis of fertility of male mice with various t-haplotypes].fertility of 47 mouse males carrying various combinations of lethal, t-haplotypes (t6/tw18, t12/tw18, tw73/tw12, tw5/tw18, t6/dt5, t12/tw12, tw5/twpa-1, tw18/twpa-1, tw5/tw12) was studied in crosses with females of different genotypes. the t-haplotypes studied belong to 7 main groups of complementation. the presence of at least two factors of fertility in the t-complex was revealed. the influence of female genotype on the degree of male fertility was also demonstrated. the data presented confirm ...19892620811
[genetic analysis of fertility of male mice with various t-haplotypes].fertility of 47 mouse males carrying various combinations of lethal, t-haplotypes (t6/tw18, t12/tw18, tw73/tw12, tw5/tw18, t6/dt5, t12/tw12, tw5/twpa-1, tw18/twpa-1, tw5/tw12) was studied in crosses with females of different genotypes. the t-haplotypes studied belong to 7 main groups of complementation. the presence of at least two factors of fertility in the t-complex was revealed. the influence of female genotype on the degree of male fertility was also demonstrated. the data presented confirm ...19892620811
influence of oxidised dietary oil and antioxidant supplementation on membrane-bound lipid stability in broiler meat.1. the effects of oxidised oil, dietary alpha-tocopherol and bha/bht-supplementation on the fatty acid composition of mitochondrial, microsomal and soluble protein fractions of broiler muscles, and on their lability to metmyoglobin/hydrogen peroxide-catalysed peroxidation were investigated. 2. oxidised oil in the broiler diets induced rapid oxidation of the membrane-bound lipids and decreased their stability towards metmyoglobin-hydrogen peroxide-catalysed peroxidation. 3. supplementation of the ...19892627662
the rat 78,000 dalton glucose-regulated protein (grp78) as a precursor for the rat steroidogenesis-activator polypeptide (sap): the sap coding sequence is homologous with the terminal end of grp78.based on the striking sequence identity between the amino acid sequence of rat steroidogenesis-activator polypeptide (sap) and the carboxyl terminus of the 78,000 dalton glucose-regulated protein (grp78), the precursor-product relationship between grp78 and sap was investigated in leydig cells. immunoblot analysis with peptide antibodies specific for grp78 and sap showed that the putative sap precursor is also immunoreactive with the anti-grp78 antibody. genomic blot hybridizations further revea ...19892628731
plasmid composition and virulence-associated factors of yersinia pestis isolates from a plague outbreak at the paraiba state, brazil.pathogenic yersinia pestis isolates were collected during a plague outbreak at the paraiba state in 1986. the y. pestis isolates were investigated for the presence of virulence-associated factors and plasmid content. all strains analysed were proficient in the expression of the vw and fraction 1 antigens, pigment adsorption and pesticin-fibrinolysin-coagulase production. a similar plasmid profile composed by four plasmid with molecular weight of 60, 44, 14.9, and 6.4 megadaltons (md) was found i ...19892629060
growth and survival of four strains of francisella tularensis in a rich medium preconditioned with acanthamoeba palestinensis.the hypothesis of positive interactions between francisella tularensis lvs (live vaccine strain) and acanthamoeba palestinensis was tested. pregrowth of the amoebae, in a rich (autoclaved and filtered) medium, from which they were subsequently removed by filtration, conditioned the medium so that growth of the live vaccine strain of f. tularensis occurred and the growth rate of one other strain was increased.19892630032
isopenicillin n synthase and desacetoxycephalosporin c synthase activities during defined medium fermentations of streptomyces clavuligerus: effect of oxygen and iron supplements.when the level of dissolved oxygen was increased to saturation in defined media fermentations of streptomyces clavuligerus, the total duration of activity of the penicillin ring cyclization enzyme, isopenicillin n synthase (ipns), was extended by at least 20 h; however, no increase in the stability of the ring expansion enzyme, desacetoxycephalosporin c synthase (daocs), was observed. consequently, the conversion of the excreted intermediate penicillin n to cephamycin c was 15-20% less efficient ...19892630033
effect of the calcium-binding protein regucalcin on the ca2+ transport system in rat liver microsomes: the protein stimulates ca2+ release.the effect of the calcium-binding protein regucalcin on the ca2+ transport system in the liver microsomes from fed rats was investigated. ca2+ transport was assayed by the method of millipore filtration to estimate microsomal 45ca2+ accumulation following addition of 10 mm adenosine triphosphate (atp). 45ca2+ uptake was retarded by the presence of regucalcin (1.0-4.0 microm). this retardation was remarkable at 1 min after regucalcin addition, while appreciable retardation was no longer seen at 5 ...19892632051
effect of the calcium-binding protein regucalcin on the ca2+ transport system in rat liver microsomes: the protein stimulates ca2+ release.the effect of the calcium-binding protein regucalcin on the ca2+ transport system in the liver microsomes from fed rats was investigated. ca2+ transport was assayed by the method of millipore filtration to estimate microsomal 45ca2+ accumulation following addition of 10 mm adenosine triphosphate (atp). 45ca2+ uptake was retarded by the presence of regucalcin (1.0-4.0 microm). this retardation was remarkable at 1 min after regucalcin addition, while appreciable retardation was no longer seen at 5 ...19892632051
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...19892632089
studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1).anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ...19892632089
development and clinical application of dna probe specific for peptostreptococcus micros.p. micros is thought to be an important pathogen in the etiology of certain inflammatory lesions, however, the role of this microorganism is uncertain due to the lack of rapid and reliable method to identify this species. the purpose of this study was to develop a dna probe specific for the detection of p. micros in order to evaluate its prevalence in oral infectious lesions. the whole genomic dna from p. micros was partially digested and inserted into the vector puc 13. four recombinant clones ...19892632133
development and clinical application of dna probe specific for peptostreptococcus micros.p. micros is thought to be an important pathogen in the etiology of certain inflammatory lesions, however, the role of this microorganism is uncertain due to the lack of rapid and reliable method to identify this species. the purpose of this study was to develop a dna probe specific for the detection of p. micros in order to evaluate its prevalence in oral infectious lesions. the whole genomic dna from p. micros was partially digested and inserted into the vector puc 13. four recombinant clones ...19892632133
long residual activity of bacillus sphaericus 1593 against culex quinquefasciatus larvae in artificial pools.the microbial larvicide, bacillus sphaericus 1593, was evaluated against culex quinquefasciatus larvae in artificial pools at huay kwang area in bangkok between march and october 1985. this study was aimed at determining the residual activity of b. sphaericus 1593 in waste water under field conditions. the larvicidal activity of b. sphaericus 1593 was found to persist for at least 5 months in artificial pools containing waste water. the populations of b. sphaericus 1593 in the test water fluctua ...19892633350
[sensitivity of yersinia pestis to antibiotics: 277 strains isolated in madagascar between 1926 and 1989].the antimicrobial susceptibility of 277 strains of yersinia pestis was studied using broth microdilution panel. in recent strains, trimethoprim, cotrimoxazole and ampicillin were the most active of the antibiotics tested (mics less than 2 mg/l). all strains were inhibited by 16 mg/l of kanamycin and sulfamethoxazole, 32 mg/l of sulphadiazine and sulfanilamide, and 64 mg/l of sulfamethoxypyridazine. doxycycline, minocycline, chloramphenicol, demeclocycline, tetracycline, oxytetracycline and chlor ...19892633713
[study of the plasmid expression of malagasy strains of yersinia pestis from 1926 through 1988]. 19892633714
[the effect of mg 2+ ions on the properties of various strains of yersinia pestis].the strains of yersinia pestis that restrict their growth on the media deficient for mg2+ ions at 37 degrees c have been found. the bacterial cell lysis is registered under these conditions. the effect of yersinia pestis own plasmids on the level of growth restriction in the absence of mg2+ ions has been studied. the phenomenon is not connected with the presence of the plasmid determining ca2(+)-dependence. the presence of 6md plasmid coding for pesticinogenicity increased the frequency of colon ...19892634816
[bacteriologic and serologic studies of an outbreak of plague in the state of paraíba, brazil].during a plaque outbreak in the borborema plateau focus (paraiba), bacteriological and serological studies were carried out in material from 452 patients (48 positives), 1,938 rodents and other small mammals (75 positives), 4,756 dogs (141 positives) and 2,047 cats (57 positives) obtained from 41 counties (out of which, 21 produced positive samples). twenty yersinia pestis strains isolated from material from 3 patients and 17 rodents, displayed biochemical reactions, virulence factors, antibioti ...19892635752
[a comparative study of the main components of the nucleolus in different populations of rat trophoblast cells during differentiation. ii. the nucleoli of the trophospongium cells, the glycogen cells and the secondary giant cells].a comparative study was performed of the arrangement of different nucleolar components during differentiation of trophoblast cell populations in the junctional zone of placenta (glycogen cells and trophospongium) and in the secondary giant cells. each cell type is characterized by specific interrelation of nucleolar components. some glycogen cells show signs of segregation of nucleolar components: strands of nucleolar components with fibrillar centers (fcs) are displaced to the periphery of the ...19892637541
[variability of the course of the posterior cerebral artery in humans].the aim of the paper has been to perform studies on the variation involving the course of the posterior cerebral artery in man. it has been intended to find out whether there is any relation between the course of artery and its branches depending on the sex and the side of the cerebral hemisphere. the use of binding anatomical terminology was designed to verify and update the nomenclature of vessels in this arterial area. the studies were carried out on 102 encephalons stemming from 47 males and ...19892639597
crude tea extracts decrease the mutagenic activity of n-methyl-n'-nitro-n-nitrosoguanidine in vitro and in intragastric tract of rats.the effects of tea extracts and their ingredients, catechins and l-ascorbic acid (asa), on the mutagenicity of n-methyl-n'-nitro-n-nitrosoguanidine (mnng) were examined in vitro and in the stomachs of rats using e. coli wp2 and s. typhimurium ta100. the extracts of green tea and black tea leaves decreased the mutagenic activity of mnng to e. coli wp2 in vitro in a desmutagenic manner. catechins such as (-)-epigallocatechin from green tea leaves and the low-molecular-weight tannin fraction isolat ...19892642597
sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy.the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ...19892643617
sensitivity and specificity of an enzyme-linked immunosorbent assay using the recombinant dna-derived treponema pallidum protein tmpa for serodiagnosis of syphilis and the potential use of tmpa for assessing the effect of antibiotic therapy.the recombinant dna-derived treponema pallidum membrane protein tmpa, purified from escherichia coli k-12, was used in an enzyme-linked immunosorbent assay (elisa) to evaluate its suitability in a screening test for syphilis and to monitor the effect of antibiotic treatment. the sensitivity of the tmpa elisa was 76% for primary syphilis, 100% for secondary syphilis, and 98% for early latent syphilis. all except 1 of 15 serum samples positive for yaws were positive in this test. a specificity of ...19892643617
lipoprotein metabolism influenced by training-induced changes in human skeletal muscle.the influence of training-induced adaptations in skeletal muscle tissue on lipoprotein metabolism was investigated in six healthy men. the knee extensors were studied at rest and during exercise after 8 wk of dynamic exercise training of the knee extensors of one leg, while the other leg served as a control. the trained and nontrained thighs were investigated on different occasions. in the trained knee extensors, muscle (m) lipoprotein lipase activity (lpla) was 70 +/- 29% higher compared with t ...19892643634
lipoprotein metabolism influenced by training-induced changes in human skeletal muscle.the influence of training-induced adaptations in skeletal muscle tissue on lipoprotein metabolism was investigated in six healthy men. the knee extensors were studied at rest and during exercise after 8 wk of dynamic exercise training of the knee extensors of one leg, while the other leg served as a control. the trained and nontrained thighs were investigated on different occasions. in the trained knee extensors, muscle (m) lipoprotein lipase activity (lpla) was 70 +/- 29% higher compared with t ...19892643634
homology between virf, the transcriptional activator of the yersinia virulence regulon, and arac, the escherichia coli arabinose operon regulator.virulent yersiniae (yersinia pestis, y. pseudotuberculosis, and y. enterocolitica) restrict their growth at 37 degrees c in rich medium deprived of calcium. this property, called calcium dependency, correlates with the secretion of yersinia outer membrane proteins (yops) and with pathogenicity. it is mediated by a 70-kilobase plasmid called pyv. the structural genes of the yops (yop genes), as well as genes involved in the control of their expression (vir genes), have been localized on pyv. in t ...19892644192
identification of cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig pancreas with light and electron microscopy.cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig were studied by light- and electron-microscopic immunocytochemistry using antisera to s-100b protein, insulin, glicentin, somatostatin, and pancreatic polypeptide. two types of s-100b-immunoreactive cells were identified. the first type was stellate and characterized by thin cytoplasmic processes sheathing endocrine-type cells, especially pancreatic a-cells. it was located predominantly in th ...19892647297
identification of cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig pancreas with light and electron microscopy.cell types containing s-100b protein-like immunoreactivity in the islets of langerhans of the guinea pig were studied by light- and electron-microscopic immunocytochemistry using antisera to s-100b protein, insulin, glicentin, somatostatin, and pancreatic polypeptide. two types of s-100b-immunoreactive cells were identified. the first type was stellate and characterized by thin cytoplasmic processes sheathing endocrine-type cells, especially pancreatic a-cells. it was located predominantly in th ...19892647297
use of megestrol acetate in cats. 19892647696
oral and pharyngeal candidiasis. topical agents for management and prevention.about half of the general population harbors candida species in oral flora, and oral candidal infections are common. however, in immunocompromised or immunosuppressed patients, candidiasis may progress to life-threatening systemic disease. patients with human immunodeficiency virus (hiv), acquired immunodeficiency syndrome, hiv disease, diabetes, or leukemia are particularly prone to serious systemic infection. chemotherapy for cancer and bone marrow and organ transplantation also provide physio ...19892648372
nucleotide sequence of the plasminogen activator gene of yersinia pestis: relationship to ompt of escherichia coli and gene e of salmonella typhimurium.we have determined the nucleotide sequence of the 1.4-kilobase dna fragment containing the plasminogen activator gene (pla) of yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. the sequence revealed the presence of a 936-base-pair open reading frame that constitutes the pla gene. this reading frame encodes a 312-amino-acid protein of 34.6 kilodaltons and containing a putative 20-amino-acid signal sequence. the presence of a single large open re ...19892651310
[detection of plague microbes in fleas using immunoenzyme technics and monoclonal antibodies].the paper presents the results of immunoassays for indication of pest causative agents in fleas, this method being compared to the classical and bacteriological methods. experiments proved the feasibility of indication of pest causative agents in fleas by immunoassays. immunoassays were found to excel the classical and bacteriological ones.19892651856
[resistance to infection. physiology and pathology of the immune system].the contribution of observations in congenital deficiency disorders to our understanding of normal mechanisms can hardly be overestimated, as may in particular be seen in the complex field of mechanisms aiming at maintaining the individual integrity. after a brief summary of the natural lines of defense we demonstrate a few cases with typical deficiencies, i.e. of the integuments (ciliary dyskinesia syndrome), of the phagocytes (chronic granulomatous disease = cgd), of specific immune reactions ...19892652037
[characteristics of the final stages of ergosterol biosynthesis in saccharomyces cerevisiae yeasts].a scheme of ergosterol cyclic intermediate transformations in s. cerevisiae has been proposed. it is based on the analysis of sterol composition in strains with mutant blocks of one or two reactions of enzymatic synthesis. biochemical reactions of modification of cyclic part and side branch of sterol molecule are supposed to be carried out independently.19892654239
the mouse bone marrow micronucleus assay can be used to distinguish aneugens from clastogens.the occurrence of aneuploidy can be measured in several assays. however, none of them have been sufficiently validated. the bone marrow micronucleus test may be considered as a method for aneuploidy detection. in this work, micronuclei were induced by two aneugens, vincristine sulfate (0.1 mg/kg) and nocodazole (80 mg/kg), and two clastogens ethylmethanesulfonate (ems) (300 mg/kg) and cyclophosphamide (60 mg/kg). three criteria have been examined in order to distinguish micronuclei induced by an ...19892654553
cytoskeletal proteins including myofilaments in human tumors. 19892660368
transferring patients with insulin-dependent diabetes mellitus from animal-source insulins to recombinant dna human insulin: clinical experience.the efficacy and safety of transferring diabetic patients being treated with animal insulin to recombinant deoxyribonucleic acid human insulin (biosynthetic human insulin) was evaluated in 481 patients. paired initial and follow-up random plasma glucose and hemoglobin alc levels were available for 330 individuals. one year or longer after transfer to human insulin, the patients' morning and evening insulin doses remained virtually unchanged. after transfer, there were significant decreases in th ...19892663162
[imported falciparum malaria in marseilles hospitals in 1987. apropos of 104 cases].patients were composed of 85 adults and 19 children; 70 were european, 23 comorian; malaria was contracted by 9 patients in french guyana, 60 in africa, 23 in comoro islands; prophylaxis was correct for 45 patients (nearly all of the cases with chloroquine) when the first symptoms occurred. every case of malaria appeared during the month following their return from an endemic area. fever was often moderate or intermittent, altered by prophylaxis and previous treatments. some patients had a clini ...19892663206
[cloning and study of the function of reca-like gene of yersinia pestis in escherichia coli cells].a 2 kb fragment of yersinia pestis genome cloned in escherichia coli cells of the strain hb101 contains a gene able to complement the reca-dependent deficiency of e. coli cells in uv-resistance, resistance to alkylating agents, uv- and mnng-induced mutability. cellular capability for homologous recombination in crosses with hfrh donor, derepressed synthesis of bacteriocins (colicin e1 and pesticin 1) is also complemented by the fragment in e. coli reca-strains. the obtained data suggest the func ...19892664489
review of cardiovascular findings in humans treated with minoxidil.minoxidil is an antihypertensive vasodilator with no known capability of inducing human cardiovascular pathology; however, the compound does cause a late, canine-specific lesion of the right atrium and acute right atrial and ventricular papillary muscle changes. in all the autopsy cases of minoxidil-treated humans to date, 9 cases were reported with significant right atrial or other cardiac pathology and were carefully reviewed for similarity to either the early or late canine lesion. three new ...19892665032
signal peptide subsegments are not always functionally interchangeable. m13 procoat hydrophobic core fails to transport alkaline phosphatase in escherichia coli.bacterial signal peptides display little amino acid sequence homology despite their shared role in mediating protein transport. this heterogeneity may exist to permit the establishment of signal peptide conformations that are appropriate for transport of particular proteins. in this paper we explore how signal peptides are composed of structural units that may interact with each other and with the mature protein to effect transport. using a new application of cassette mutagenesis, we have replac ...19892668291
a new tool for the serodiagnosis of acute plasmodium falciparum malaria in individuals with primary infection.we have developed an elisa which detects, with high specificity, antibodies against a major surface protein of p. falciparum merozoites which is a processing product of the precursor glycoprotein gp190. this assay can be used in the diagnosis of acute malaria in individuals with primary infection. two partial sequences of gp190 were expressed in e. coli as beta-galactosidase (beta-gal) fusion proteins. the same sequences fused to chloramphenicol acetyltransferase (cat) or mouse dihydrofolate red ...19892668417
fungistatic activity of miconazole against candida albicans in relation to ph and concentration of nonprotonated drug.at less than 10(-5) m, miconazole (mcz) exerts a fungistatic effect on candida albicans, presumably by interfering with ergosterol biosynthesis. the imidazole moiety of mcz is subject to protonation (pka approximately 6.5). based on pka and greater water solubility of protonated (mczh+) versus nonprotonated (mczo) drug, fungistatic action ought to be markedly affected by environmental ph, but apparently it is not. in this report growth phase, ph, and concentrations of mcz and mczo have been stud ...19892668754
liver disease associated with exposure to 1,1,1-trichloroethane.1,1,1-trichloroethane is a halogenated hydrocarbon solvent commonly used in industry because of its supposed lack of hepatotoxicity. nonetheless, animal studies performed by several independent groups have shown the solvent to induce fat deposition, vacuolar degeneration, and centrilobular necrosis, changes similar to those seen after exposure to carbon tetrachloride, albeit of a much reduced magnitude, in animals exposed to the agent. four patients with fatty liver disease whose work entailed s ...19892669664
heterogeneity of responses to lh-releasing hormone and phorbol ester among rat gonadotrophs: a study using a reverse haemolytic plaque assay for lh.protein kinase c (pk-c) has been implicated in the action of lhrh because lhrh-induced release of lh is partially mimicked by phorbol esters which activate pk-c, and reduced in magnitude by inhibitors of pk-c. we have used a reverse haemolytic plaque assay for lh to visualize and compare the direct effects on individual rat gonadotrophs of (1) a 2-h exposure to a range of concentrations of lhrh and phorbol 12-myristate 13-acetate (pma) and (2) a single maximally stimulatory dose (10 nm) of lhrh ...19892669809
the yopm gene of yersinia pestis encodes a released protein having homology with the human platelet surface protein gpib alpha.in yersinia pestis kim, there are 11 yops (yersinial outer membrane proteins) encoded by the low-ca2+ response virulence plasmid pcd1. only yops m and n are found in easily detectable amounts in the culture medium. in this study, we located and characterized the yopm gene to obtain clues about its role in the virulence of y. pestis. rabbit antibody was raised against yops m and h, copurified from the supernatant of y. pseudotuberculosis 43(pgw600, pcd1 yope::mu di1[apr lac]). this antiserum was ...19892670888
expression and partial purification of human pro-opiomelanocortin in escherichia coli.a large portion of the human pro-opiomelanocortin (pomc) peptide corresponding to amino acid residues 59-241 has been cloned and expressed in escherichia coli. a 1.0 kb dna fragment encoding this peptide was cloned into the expression vectors puc8 and pur291. plasmid pjmbg51 (a puc8 recombinant) was found to direct the expression of a 24 kda peptide. the recombinant pur291 (pjmbg52) was shown to produce a beta-galactosidase fusion protein of 140 kda. western blot analysis showed that both the 24 ...19892673285
high affinity insulin binding in the human placenta insulin receptor requires alpha beta heterodimeric subunit interactions.insulin binding to human placenta membranes treated at ph 7.6 or 8.5 in the presence or absence of 2.0 mm dtt for 5 min, followed by the simultaneous removal of the dtt and ph adjustment to ph 7.6, displayed curvilinear (heterogeneous) insulin binding plots when analyzed by the method of scatchard. however, triton x-100 solubilization followed by bio-gel a-1.5m gel filtration chromatography of the placenta membranes previously treated with dtt at ph 8.5 generated a nearly straight line (homogene ...19892674449
modeling of palmitate transport in the heart.transport of palmitate from the albumin-palmitate complex in the plasma to inside mitochondria where it undergoes beta-oxidation is a multistep process. albumin's large size prevents permeation via interendothelial clefts. palmitate dissociation from albumin in solution is too slow to provide an adequate supply of the unbound palmitate. the discovery that the dissociation occurs upon albumin binding to an endothelial surface receptor resolves the conundrum. palmitate transport across the luminal ...19892674667
[hospital strains of salmonella serotype typhimurium with particular biological properties].93 cultures of salmonella typhimurium (stm) were isolated from the infants (under one year old) admitted into a pediatrics ward. all the cultures had only flagellation antigens in phase i, a common sensitivity spectrum to antibiotics and the same sensitivity to the sets of bacteriophages for typing of this serotype. of these, 10 strains were negative h2s and 6 immobile. using as carrier a strain of e. coli, k12nxr, an experimental transfer of multiple resistance to the antibiotics obtained from ...19892675270
detection of minimal residual disease in acute lymphoblastic leukemia by in vitro amplification of rearranged t-cell receptor delta chain sequences.human t-cell receptor (tcr) delta-chain diversity mainly originates from high junctional variability, since only a limited number of germline elements is available. this extraordinary diversity at the v.j junction, due to the use of two d delta elements and extensive incorporation of n nucleotides, constitutes a specific clonal marker for cell populations exhibiting rearranged tcr delta genes. to this end we amplified in vitro by polymerase chain reaction (pcr) the tcr delta junctional region of ...19892676018
cloning of a dna sequence encoding a major fragment of the 47 kilodalton stress protein homologue of candida albicans.part of the gene coding for the immunodominant 47 kda antigen of the human fungal pathogen candida albicans was cloned and sequenced. the predicted amino acid sequence was homologous to stress proteins, the most extensive similarity being with the heat shock protein hsp 90 of saccharomyces cerevisiae. the 47 kda antigen has diagnostic and therapeutic potential and is the first candidal antigen to be cloned and sequenced.19892676706
genomic footprinting of a yeast trna gene reveals stable complexes over the 5'-flanking region.we have shown by genomic footprinting that the 5'-flanking region of the saccharomyces cerevisiae trnasup53 gene is protected from dnase i digestion. the protected region has a 5' boundary at -40 (relative to the transcription initiation site) and extends into the coding region of the gene, with a 3' boundary at approximately +15. although the dnase i protection over this region was much greater than at the a- and b-box internal promoters, point mutations within the a or b box that reduced trans ...19892677668
haemostatic changes caused by the venoms of south american snakes.changes in the haemostatic mechanism caused by venoms of bothrops, crotalus and lachesis snakes from central and south america in human accidents are reviewed. changes in the blood coagulation mechanism could be found depending on the action of the venom on clotting factors.19892678605
interrelationships between digestive proteolytic activities and production and quantitation of toxins in pseudomembranous colitis induced by clostridium difficile in gnotobiotic mice.clostridium difficile pathogenicity is related to in vivo production of toxins, and it is of great interest to detect toxins produced in biological samples. several reports have shown that proteases in stools interfere with immunological methods for quantitation of toxin a. the purpose of this work was to estimate the relationship between the proteases and the c. difficile toxins produced in a gnotobiotic mouse model of pseudomembranous cecitis. cecal proteolytic activities hydrolyzed toxin a, a ...19892680988
advanced pancreatic cancer: a phase i-ii trial of cisplatin, high-dose cytarabine, and caffeine.in a phase i-ii study, 28 patients with advanced pancreatic adenocarcinoma were treated with cisplatin, high-dose cytarabine (ara-c), and caffeine. this clinical trial was based on a nude mouse-human tumor xenograft model, which demonstrated synergism of these agents by inhibiting the growth of human pancreatic tumors. toxic effects noted in the clinical study included myelosuppression, moderate nausea and vomiting, and mild renal insufficiency. no toxic effects were directly attributable to caf ...19892681796
[expression of pre-s2 antigen and antibody in patients with hepatitis b virus infection].we investigated the expression of pre-s2 antigen (ag) and antibody (ab) in sera quantitatively using elisa. four patients with acute hepatitis b and 87 chronic hbv carriers were included in this study. we also investigated the expression of pre-s2ag in the liver tissues obtained from 26 chronic hbv carriers by direct fluorescent antibody method. there was a significant correlation between pre-s2ag titers and hbsag titers in sera. pre-s2ag titers were higher in sera positive for hbeag, hbv-relate ...19892681890
[coexistence of abdominal aortic aneurysm and intraabdominal malignancy; two case reports].we experienced two cases of abdominal aortic aneurysm which had intra-abdominal malignancy (early gastric cancer). case 1 was a 72 year-old man who was treated by two-stage operation of them. gastrectomy was performed about 7 months prior to the aneurysmectomy. case 2 was a 70 year-old man who was diagnosed both lethal diseases, renal dysfunction, chronic respiratory failure and multiple ventricular arrhythmia. he was treated by one-stage operation and was discharged with no complications. coexi ...19892682201
[magnetic immunoenzyme analysis of yersinia pestis antigens].the detection of y. pestis cells in magnetic enzyme immunoassay is carried out with the use of magnetic polyacrylamide microgranules. in the assay system for the determination of the antigen commercial y. pestis antigens, peroxidase-labeled antibodies, the substrate mixture consisting of sodium salt of 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and h2o2 in citrate-phosphate buffer solution, ph 4.5, are used. the sensitivity of the method is 5 x 10(4) microbial bodies per ml.19892683524
redox regulation of the genes for cobinamide biosynthesis in salmonella typhimurium.transcription of the cobinamide biosynthetic genes (the cobi operon) was induced under three different physiological conditions: anaerobiosis (anaerobic respiration or fermentation), aerobic respiration at low oxygen levels, and aerobic respiration with a partial block of the electron transport chain. after a shift to inducing conditions, there was a time lag of approximately 50 min before the onset of cobi induction. under conditions of anaerobic respiration, the level of cobi transcription was ...19892687249
sodium-transport nadh-quinone reductase of a marine vibrio alginolyticus.the respiratory chain of a marine bacterium, vibrio alginolyticus, required na+ for maximum activity, and the site of na+ -dependent activation was localized on the nadh-quinone reductase segment. the na+ -dependent nadh-quinone reductase extruded na+ as a direct result of redox reaction. it was composed of three subunits, alpha, beta, and gamma, with apparent mr of 52, 46, and 32 kda, respectively. the reduction of ubiquinone-1 to ubiquinol proceeded via ubisemiquinone radicals. the former reac ...19892687259
assay for sialidase using erythrocytes and peroxidase-labeled peanut lectin.a rapid and sensitive sialidase assay method based on peroxidase-labeled peanut lectin (pna) binding to desialylated erythrocyte is described. formalinized sheep erythrocytes were used both as a stable substrate for sialidase and as a target for the lectin. in the case of sialidases from vibrio cholerae and arthrobacter ureafaciens, a linear relationship was observed between the amount of peroxidase-labeled pna bound to erythrocytes and the enzyme amount. binding of the lectin to sialidase-treat ...19892688936
nutritional anestrus in beef cows: concentrations of glucose and nonesterified fatty acids in plasma and insulin in serum.twenty-two nonlactating hereford cows exhibiting normal estrous cycles were fed either maintenance (m) or restricted (r) diets until most of the r cows became anestrus; r cows then were fed 160% of the m diet until estrous cycles resumed. concentrations of progesterone, glucose, insulin and nonesterified fatty acids (nefa) were determined in weekly blood samples. blood also was collected frequently, before and after i.v. infusion of 300 ml of a 40% glucose solution, to evaluate responses in bloo ...19892689418
[biosynthetic human insulin and its clinical implication]. 19892691729
exercise training produces changes in free and conjugated catecholamines.in dogs the concentrations of conjugated dopamine in plasma have previously been shown to increase after exercise training. this study was done to determine whether conjugated norepinephrine and epinephrine also increase. fifteen dogs were randomly divided into training (n = 8) or sedentary (n = 7) groups. all dogs were exercised acutely for 5 min at 4 mph with a 12% grade, following a 3-min warm-up, before and after either a 12-wk training or a 12-wk sedentary period. free and conjugated catech ...19892691817
falciparum malaria--present day problems. an experience with 425 cases.clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...19892693436
[interleukin-1-inducing activity of the polysaccharide-containing antigens of the cell wall in yersinia pestis].the induction of the synthesis of interleukin-1 (il-1) in human monocytes under the influence of the endotoxic preparations (lps) and y. pestis basis somatic antigen has been experimentally studied. the results obtained in this study make it possible to come to the conclusion that the capacity of the endotoxin of y. pestis cell wall, consisting of lps of type r, for inducing the synthesis of il-1 in human monocytes is not different from the corresponding capacity of salmonella and shigella lps, ...19892694696
ion exchange paper adsorption and thin-layer chromatography for detection of chloroquine in urine for the study of chemosensitivity of plasmodium falciparum.with a view to determining the chemosensitivity of plasmodium falciparum to chloroquine, two qualitative tests for the detection of this antimalarial agent in urine were compared in central african schoolchildren: the test of dill and glazko and a thin-layer chromatography technique involving ion exchange and extraction. results obtained with 149 subjects showed the marked superiority in specificity and sensitivity of the chromatography test. this is of particular interest when selecting subject ...19892694983
differential diagnosis of severe periodontal lesions.in current clinical practice, a differential diagnosis of severe localized periodontal lesions is rarely made; such lesions are considered to be manifestations of periodontitis caused by specific microbes from the commensal oral flora. however, deep seated lesions of the periodontium which are in communication with the alveolar crest, are well documented periodontal consequences of pulpal pathoses and can mimic the signs and symptoms of 'periodontitis'. the very low incidence of tooth-threatenin ...19892695026
differential diagnosis of severe periodontal lesions.in current clinical practice, a differential diagnosis of severe localized periodontal lesions is rarely made; such lesions are considered to be manifestations of periodontitis caused by specific microbes from the commensal oral flora. however, deep seated lesions of the periodontium which are in communication with the alveolar crest, are well documented periodontal consequences of pulpal pathoses and can mimic the signs and symptoms of 'periodontitis'. the very low incidence of tooth-threatenin ...19892695026
cardiovascular and respiratory effects of an opioid kappa agonist ethylketazocine and sigma agonist n-allylnormetazocine in acutely decerebrated dogs.effects of opioid kappa agonist ethylketazocine (ekc), sigma agonist (+-)-n-allylnormetazocine (nanm), and naloxone alone and in combination on mean blood pressure (mbp), heart rate (hr), respiratory rate (rr) and minute volume (mv) were studied in acutely decerebrated dogs. ekc (0.5 mg/kg) decreased hr, mbp, rr and mv. post-ekc nlx increased rr and mv and reversed the bradycardia and hypotension produced by ekc. nanm (1 mg/kg) produced respiratory depression and tachycardia without changing mbp ...19892695944
influence of capsaicin-induced denervation on neurogenic and humoral control of arterial pressure.(1) the influence of capsaicin-sensitive afferent neurones on the regulation of blood pressure by reflex noradrenergic responses and by activation of the renin-angiotensin system was investigated in the rat anaesthetized with pentobarbital. (2) lowering the pressure in the carotid sinus through unilateral carotid occlusion caused a reflex rise in mean systemic blood pressure which was less marked in capsaicin-pretreated rats than in controls, although an equal drop in mean pressure in the caroti ...19892699355
the effect of dietary fatty acid composition on liver retinyl ester (vitamin a ester) composition in the rat.the dependence of liver retinyl ester (vitamin a ester) composition on dietary fatty acid composition was studied in rats. weanling male sprague-dawley rats were fed purified test diets containing 8% of one of 10 test triglycerides (either tributyrin, tricaproin, tricaprylin, tricaprin, trilaurin, trimyristin, tripalmitin, tristearin, triolein, trilinolein) or corn oil (a mixed triglyceride) for 23 d after an initial 1-wk stabilization period. essential fatty acids were provided by safflower oil ...19892703916
evidence for the development of human hydatid daughter cysts from protoscoleces. 19892704729
streptococcus pneumoniae endocarditis presenting as an epidural abscess.streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ...19892704929
streptococcus pneumoniae endocarditis presenting as an epidural abscess.streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ...19892704929
analysis of healing after myocardial infarction using polarized light microscopy.to better understand the healing process after permanent coronary artery occlusion in a canine model, the authors used polarized light microscopy. at 6 weeks after occlusion the scar collagen was mainly type i. some regions of the scar contained a fiber lattice which appeared to be type iii collagen. collagen orientation was measured using a universal stage; subepicardial collagen was obliquely aligned (-14.0 +/- 3.5 degrees), midmyocardial collagen circumferentially aligned (1.4 +/- 0.4 degrees ...19892705508
retinoids in the bovine pineal gland.using high performance liquid chromatography, the level of retinoids was determined in the bovine pineal gland, retina, retinal pigment epithelium, cortical and subcortical brain tissues, skeletal muscle and the liver. except the retina, the bovine pineal gland possesses levels of retinol and retinyl esters significantly higher than other brain tissues and muscle. however, unlike the retina, the bovine pineal gland does not possess a significant level of retinal, the chromophore for visual pigme ...19892706537
lcrh, a gene necessary for virulence of yersinia pestis and for the normal response of y. pestis to atp and calcium.we are investigating the functions of the three proteins encoded by the v operon (lcrgvh) of the low-calcium response virulence plasmid pcd1 of yersinia pestis kim5. the purpose of this study was to define the role of the 18-kilodalton protein encoded by lcrh, the third gene of the v operon. using marker exchange mutagenesis, we constructed a y. pestis mutant that failed to express the lcrh protein. this lcrh- mutant was "atp blind" in that it failed to show altered growth and v-antigen expressi ...19892707857
preliminary notes on vulnerability of the retina to injury from peripheral laser exposure conditions.if laser exposures to the periphery of the retina from low-energy lasers cause a hemorrhage obscuring the optical pathway to the fovea, resulting in loss of visual function, the risks for soldiers on the battlefield may be greater than previously expected. two off-axis experiments were undertaken to obtain an indication of the hazards involved in exposing the peripheral retina to visible laser irradiation. both off-axis exposures caused immediate vitreal hemorrhages in the periphery. after 80 mi ...19892708055
hormonal control of neuron number in sexually dimorphic spinal nuclei of the rat: ii. development of the spinal nucleus of the bulbocavernosus in androgen-insensitive (tfm) rats.the spinal nucleus of the bulbocavernosus (snb) is a sexually dimorphic motor nucleus whose development is under the control of steroid hormones. the snb contains many more motoneurons in adult male rats than in females, and this sex difference is produced by a sexually dimorphic motoneuron death which is regulated by androgens. to study further the role of androgens in the development of sex differences in snb motoneuron number, we examined snb development in males with the testicular feminizat ...19892708570
[prostaglandins and cyclic nucleotides in the dynamic development of an experimental poisoning syndrome caused by yersinia pestis lipopolysaccharide].this work deals with the influence of y. pestis lipopolysaccharide (lps), introduced intraperitoneally in a dose of 2 ld50, on the content of prostaglandins (pg), such as pge, pgf2 alpha and 6-keto-pgf1 alpha, thromboxane, camp and cgmp in the liver, lungs and blood plasma of guinea pigs in the process of the development of experimental intoxication. the content of thromboxane in blood plasma increased 2.4-fold in 2 hours after intoxication and remained elevated for as long as 5 hours. other par ...19892711790
testing a possible cocataractogenic potential of befunolol (glauconex) with animal cataract models.befunolol was tested in an animal experiment with 2 cataract models of the sprague-dawley-rat. the treatment scheme was designed with 8 different treatment groups, such that befunolol could be tested single and in combination with one or two cataract models. over the 6 weeks experimental period, slit lamp microscopy and scheimpflug photography were employed as observation and documentation methods, extensive post-mortem biochemistry was carried out after sacrifice of the animals. neither the in- ...19892715676
isolation of human platelets from plasma by centrifugation and washing. 19892716531
[viral infection and obstructive cholangiography in children].in the last years it has been suggested that idiopathic obstructive cholangiopathy is a pathologic process with evolutive studies: extrahepatic biliary atresia and neonatal hepatitis. the possibility of a viral agent as the inciting factor has been investigated. this report shows four male and one female with confirmed idiopathic obstructive cholangiopathy with different clinical picture. there was evidence of cytomegalovirus infection in two patients, rubella infection in one patient, hepatitis ...19892719422
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...19892721224
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...19892721224
identification of 9-hydroxylamine-1,2,3,4-tetrahydroacridine as a hepatic microsomal metabolite of tacrine by high-performance liquid chromatography and electrochemistry.amperometric detection using a dual-electrode thin-layer cell in the series configuration can aid in the identification of unknown components in complicated samples by voltammetric characterization. this is shown by studying the metabolism of tacrine by rat hepatic microsomes using high-performance liquid chromatography with electrochemical detection. the major metabolite detected in microsomal incubations did not co-elute with any standard acridine available and was produced in too small a quan ...19892723005
suncus murinus. observations on ecology, distribution, status to plague in bombay.the common house shrew suncus murinus has been shown to play an important role in maintenance and perpetuation of plague infection by earlier plague workers. with the control of human plague there is no knowledge about foci of plague in small mammals associated with man. present study was carried out to fill in this lacuna. studies carried out in the present paper reveal that s. murinus does not harbour any plague infection in bombay. this species is widely distributed in bombay and is found to ...19892723422
synthesis and biological evaluation of s-adenosyl-1,12-diamino-3-thio-9-azadodecane, a multisubstrate adduct inhibitor of spermine synthase.as part of a continuing search for specific inhibitors of the enzymes involved in polyamine biosynthesis, we have designed and synthesized a multisubstrate adduct inhibitor, s-adenosyl-1,12-diamino-3-thio-9-azadodecane (adodatad), in which critical portions of the nucleophilic aminopropyl acceptor are covalently linked to critical portions of the electrophilic aminopropyl donor to form a potent and specific inhibitor of spermine synthase. in addition, the corresponding desamino analogue which wa ...19892724300
human immunodeficiency virus infection among employees in an african hospital. 19892725605
mycoplasma infection of cultured cells.mycoplasma contamination is tough to detect and even more difficult to eradicate. it is best to start over fresh from clean cell stocks, but several elimination options are available.19892725683
disinhibition by propranolol and chlordiazepoxide of nonrewarded lever-pressing in the rat is unaffected by dorsal noradrenergic bundle lesion.ten male sprague-dawley rats received 6-hydroxydopamine-induced lesions of the dorsal noradrenergic bundle and 10 others underwent control operations. the lesion depleted levels of noradrenaline in the hippocampus to 2% of those in the controls. all rats were then trained for 16 sessions to lever-press in a skinner box on a variable interval 18 sec schedule of food-reinforcement, then for 42 days on a successive discrimination between periods of variable interval (vi 18 sec) food-reinforcement a ...19892725847
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