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[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...19902238764
[the immune status of children in foci of measles infection studied by an immunoenzyme method].children immunized with live measles vaccine in the foci of measles infection varying in intensity (1-9 cases per focus) have been subjected by two methods: the hemagglutination inhibition (hai) test and the enzyme immunoassay (eia). as shown in this study, in most cases (98% of all blood serum samples) the correlation between the results of the hai test and eia is not high (r = 0.5), which is linked with the detection of a wider spectrum of antibodies in eia. the percentage of seronegative chil ...19902239001
[the immune status of children in foci of measles infection studied by an immunoenzyme method].children immunized with live measles vaccine in the foci of measles infection varying in intensity (1-9 cases per focus) have been subjected by two methods: the hemagglutination inhibition (hai) test and the enzyme immunoassay (eia). as shown in this study, in most cases (98% of all blood serum samples) the correlation between the results of the hai test and eia is not high (r = 0.5), which is linked with the detection of a wider spectrum of antibodies in eia. the percentage of seronegative chil ...19902239001
recombinant capsular antigen (fraction 1) from yersinia pestis induces a protective antibody response in balb/c mice.yersinia pestis produces a glycoprotein capsule, the biosynthesis of which appears to be temperature dependent. the fraction i (f1) component of this capsule is specific to y. pestis and the detection of f1 antibodies is the basis for several serological tests. we report the cloning of the f1 gene and its expression in escherichia coli using the phagemid vector lambda zapii and a f1-specific monoclonal antibody. the recombinant f1 antigen had a molecular weight of 17 kda, which proved to be iden ...19902240367
[differentiation between in-life and post-mortem hemorrhage].the determination of the time of haemorrhaging can be of substantial importance in the forensic practice. histological, immunohistochemical and morphometric investigations were carried out on haemorrhages from animal experiments and from human pathological samples. centrifugal topology, serum-erythrocyte discontinuity, topological arrangement of fibrin formation and stabilisation of factor xiii a were evaluated as criteria to define antemortal origin of haemorrhages.19902241826
the use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. detection of picomolar quantities by an imaging system based on a cooled charge-coupled device.various monosaccharides, oligosaccharides and small polysaccharides were labelled covalently at their reducing end groups with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid (ants), and the resulting fluorescent derivatives were separated by high-resolution page. the electrophoretic mobilities of the labelled saccharides are related largely to the compounds' mr values, but they are also influenced by the individual chemical structures of the saccharides. various positional isomers an ...19902241903
the use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid. detection of picomolar quantities by an imaging system based on a cooled charge-coupled device.various monosaccharides, oligosaccharides and small polysaccharides were labelled covalently at their reducing end groups with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid (ants), and the resulting fluorescent derivatives were separated by high-resolution page. the electrophoretic mobilities of the labelled saccharides are related largely to the compounds' mr values, but they are also influenced by the individual chemical structures of the saccharides. various positional isomers an ...19902241903
analysis of immunosuppressive molecules associated with murine in vitro fertilized embryos.supernatants from mouse in vitro fertilized (ivf) oocyte cultures may suppress in vitro lymphocyte proliferation stimulated by concanavalin a (sigma, st. louis, mo). supernatants conditioned by incubation with mouse epididymal sperm alone were even more inhibitory. thin-layer chromatography suggested the polyamines spermine in sperm and spermine plus spermidine in ivf embryo supernatants were responsible. putrescine was not suppressive. in vitro fertilized oocytes from old cba/j-strain mice (gre ...19902245843
electronic determination of size and number in isolated unfixed adipocyte populations.adipose tissue cellularity and metabolism are traditionally expressed in terms of mean cell size and number. the need for a simple method allowing rapid determination of cell size and number of freshly isolated, unfixed adipocyte preparations led us to compare estimates of cell size determined by the established method of optical sizing to a proposed method of electronic cell sizing and counting. in collagenase-isolated, unfixed adipocytes whose mean diameters ranged from approximately 40 to 65 ...19902246619
electronic determination of size and number in isolated unfixed adipocyte populations.adipose tissue cellularity and metabolism are traditionally expressed in terms of mean cell size and number. the need for a simple method allowing rapid determination of cell size and number of freshly isolated, unfixed adipocyte preparations led us to compare estimates of cell size determined by the established method of optical sizing to a proposed method of electronic cell sizing and counting. in collagenase-isolated, unfixed adipocytes whose mean diameters ranged from approximately 40 to 65 ...19902246619
a simplified assay for measurement of cytosine arabinoside incorporation into dna in ara-c-sensitive and -resistant leukemic cells.the assays for the detection of unlabeled 1-beta-d-arabinofuranosylcytosine (cytosine arabinoside, ara-c) incorporation into dna was simplified. the procedure includes dna isolation from leukemic cells, quantification of dna concentrations, breakdown by enzymatic digestion of dna to nucleosides and a radioimmunoassay (ria) using an antibody against ara-c. different techniques for quantification of dna concentrations are compared. a fluorimetric technique using hoechst 33258 is preferred because ...19902249332
[genus-specific dna probe for detection of yersinia].in order to create a rdna probe for plague agent (yersinia pestis) double-stranded dna fragments complementary to 5'-region of 16s rrna were synthetized with the help of reverse transcriptase. the fragments were cloned into plasmid vector puc19 in escherichia coli. to select plasmids with specific for y. pestis sequences, recombinant clones and plasmids purified from them were cross-hybridized to [gamma-32 p]-labelled 16s rrna of e. coli and y. pestis. as was shown after sequencing of recombinan ...19902250669
enzyme immunosorbant assay of oestradiol in unextracted plasma using penicillinase as label.an enzyme-linked immunosorbant assay (elisa) for measuring oestradiol directly in plasma without extraction utilizing antibodies raised against oestradiol-3-(o-carboxymethyl) ether-bovine serum albumin conjugate, and oestradiol-6-(o-carboxymethyl) oxime linked to penicillinase (ec 3.5.2.6) as a marker was developed. polyvinyl 96-well microtitre plates were used for immobilization of anti-oestradiol igg. standards of oestradiol (92 to 9,190 pmol/l were prepared in oestradiol-free plasma and 8-ani ...19902253397
[the effect of different yersinia pestis antigens on the cellular link in immunity].immunization with live plague vaccine has been shown to give no protection to thymectomized mice from subcutaneous challenge with y. pestis virulent strain. under the action of the vaccine or individual y. pestis antigens (fraction i) the functional and morphological activation of thymocytes and macrophages is observed, more pronounced in c57bl/6 mice and less pronounced in cba mice. y. pestis antigenic preparations (fractions i and ii, pesticin) act as t-cell mitogens and are thus capable of in ...19902256408
[the role of bacteriophages in the self-regulating system of a plague enzootic]. 19902256411
effects of ibudilast on calcium deposition in soft tissues of rabbits with atherosclerosis.the effects of 2.5 mg/kg.day ibudilast, with or without 300 mg/kg.day nicomol, or 10 mg/kg.day ibudilast on calcium and magnesium concentrations in the central nervous system (cns), heart, liver, kidney, skeletal muscle, abdominal aorta and bone were determined in rabbits fed a 1% cholesterol-rich diet for 3 months. serum thromboxane b2 concentrations were reduced in rabbits treated with 10 mg/kg.day ibudilast or with 2.5 mg/kg.day ibudilast plus 300 mg/kg.day nicomol, and atherosclerotic lesion ...19902257963
functional studies examining the subpopulation of human b lymphocytes responding to high molecular weight b cell growth factor.mature human b lymphocytes perform many functions including antibody secretion, ag presentation, preservation of memory for ag, and lymphokine secretion. individual resting b cells receive multiple sequential signals that determine the function(s) that will be performed by those cells. activation signals such as ag or staphylococcus aureus cowan i (sac) stimulate overlapping but different subpopulations of b cells. after activation, b cells may be induced to proliferate by a variety of b cell gr ...19902258603
functional studies examining the subpopulation of human b lymphocytes responding to high molecular weight b cell growth factor.mature human b lymphocytes perform many functions including antibody secretion, ag presentation, preservation of memory for ag, and lymphokine secretion. individual resting b cells receive multiple sequential signals that determine the function(s) that will be performed by those cells. activation signals such as ag or staphylococcus aureus cowan i (sac) stimulate overlapping but different subpopulations of b cells. after activation, b cells may be induced to proliferate by a variety of b cell gr ...19902258603
vascular permeability factor: a tumor-derived polypeptide that induces endothelial cell and monocyte procoagulant activity, and promotes monocyte migration.systemic infusion of low concentrations of tumor necrosis factor/cachectin (tnf) into mice that bear tnf-sensitive tumors leads to activation of coagulation, fibrin formation, and occlusive thrombosis exclusively within the tumor vascular bed. to identify mechanisms underlying the localization of this vascular procoagulant response, a tumor-derived polypeptide has been purified to homogeneity from supernatants of murine methylcholanthrene a-induced fibrosarcomas that induces endothelial tissue f ...19902258694
pneumocystis carinii pneumonia in danish piglets. 19902267715
does emotional eating interfere with success in attempts at weight control?questionnaire responses from a convenience sample were used to test for hypothesized relationships between changes over time in individuals' reported frequency of emotional eating and estimates of their success in attempts to reduce body weight over periods of at least 1 year. respondents were 187 english adults, whose distribution of estimated body mass indices (bmi) approximately that of the general population. initial bmi was significantly (p less than 0.001) positively associated with report ...19902268140
endothelin releasing activity in calf serum and porcine follicular fluid.bovine calf serum (cs) and porcine follicular fluid (pff) added to bovine aortic endothelial cells (baec) in culture stimulate endothelin (et) release. sorbent extraction of acidified calf serum and pff using bond elut c18 yields a product with an activity 30% that of the starting material. fractionation of bond elut c18 extracted cs or pff using hplc yields peaks of activity with retention times similar to those of tgf beta and activin a, respectively. tgf beta is 10-fold more potent than activ ...19902268344
[trimethoprim resistance--a differential trait of strains of yersinia pestis from a variety of voles].strains of all y. pestis varieties except for those isolated from voles are highly sensitive to trimethoprim when grown on cultivation media, even when thymidine is added to the medium. the ability to grow on thymidine-containing rich media after trimethoprim addition is a property differentiating y. pestis variety isolated from voles from the other varieties. these data were confirmed by testing 360 y. pestis strains obtained from collections of several antiplague institutes and stations of the ...19902273998
[study of protective properties of antigen-containing liposomes of different lipid composition in plague].immunization of animals with a liposomal capsular and major somatic antigen of a plague causative agent enhanced their protective effects against plague infection. lipopolysaccharide incorporation into the lipid membrane resulted in an increased protective effect of antigen-containing liposomes, which may be related to the single delivery and interaction with immune-competent cells. incorporation of phospholipids with high phase transfer temperature and positively charged stearylamine by the mem ...19902275277
transcriptional activation and dna binding by the erythroid factor gf-1/nf-e1/eryf 1.the murine, erythroid dna-binding protein gf-1 (also known as nf-e1, eryf 1), a 413-amino acid polypeptide with two novel finger domains of the cx-cx variety, recognizes a consensus gata motif present in cis elements of the majority of erythroid-expressed genes. we have performed a structure-function analysis of this protein to evaluate its potential as a transcriptional activator and to examine the role of the finger domains in dna binding. using a cotransfection assay, we find that gf-1 is a p ...19902276623
transcriptional activation and dna binding by the erythroid factor gf-1/nf-e1/eryf 1.the murine, erythroid dna-binding protein gf-1 (also known as nf-e1, eryf 1), a 413-amino acid polypeptide with two novel finger domains of the cx-cx variety, recognizes a consensus gata motif present in cis elements of the majority of erythroid-expressed genes. we have performed a structure-function analysis of this protein to evaluate its potential as a transcriptional activator and to examine the role of the finger domains in dna binding. using a cotransfection assay, we find that gf-1 is a p ...19902276623
bone allografts sterilised by irradiation. biological properties, procurement and results of 150 massive allografts.massive bone allografts from cadavers have been sterilised by gamma-radiation from radioactive cobalt at a dose of 25,000 gray (gy). the biological effects of radiation are discussed. human cortical bone showed an acceptable 20% decrease in strength on bending tests after 27,000 gy irradiation, but higher doses are more damaging and should be avoided. the procurement protocol used at the cochin hospital is described, and the importance of dosimetry and record-keeping emphasised. the clinical res ...19902279829
accelerated restoration of the leukocyte number and augmented resistance against escherichia coli in cyclophosphamide-treated rats orally administered with a hot water extract of chlorella vulgaris.the effects of oral administration of a hot water extract of chlorella vulgaris (cve) on the restoration of the leukocyte number and on the resistance against escherichia coli infection were examined in cyclophosphamide (cy)-treated rats. male fischer rats (f344/ducrj) were administered orally 1000 mg/kg of cve for 14 days and injected intraperitoneally with a single dose of cy (50 mg/kg) (day 0) one day after the 14th cve administration. cve was further administered continuously after cy treatm ...19902292465
accelerated restoration of the leukocyte number and augmented resistance against escherichia coli in cyclophosphamide-treated rats orally administered with a hot water extract of chlorella vulgaris.the effects of oral administration of a hot water extract of chlorella vulgaris (cve) on the restoration of the leukocyte number and on the resistance against escherichia coli infection were examined in cyclophosphamide (cy)-treated rats. male fischer rats (f344/ducrj) were administered orally 1000 mg/kg of cve for 14 days and injected intraperitoneally with a single dose of cy (50 mg/kg) (day 0) one day after the 14th cve administration. cve was further administered continuously after cy treatm ...19902292465
[osteoporosis in monkeys after anti-orthostatic hypokinesia].using methods of quantitative histomorphometry, the effect of 15- and 30-day exposure to antiorthostatic hypokinesia (-10 degrees) on spongy bone of iliac crests and proximal metaphysis of tibia of macaca mulatta was examined. it was found that the exposure caused osteoporosis of bone spongiosa. osteoporotic changes increased progressively with time of exposure. osteoporosis developed as a result of stimulated bone resorption, which was indicated by a greater resorption surface and a higher acti ...19902292868
[glucocorticoid receptors in physiological states and in extreme conditions].this review discusses glucocorticoid hormone--receptor interaction in the animal and human organism in the normal, extreme (stress, radiation, head-down tilt, etc.) and pathological situations. particular attention is given to advanced lines of research into glucocorticoid receptors within the framework of space biology and medicine. it is stressed that the data about glucocorticoid receptors may form the key that can help better understanding of changes induced by exposure to the space environm ...19902292872
a sensitive in vitro functional assay to detect k(+)-channel-dependent vasodilators.this study describes a sensitive in vitro relaxation assay using isolated rabbit mesenteric artery to detect the activity of a vasodilator as a k(+)-channel activator. thus, comparison of several known k(+)-channel activators was made with other vasodilators known to work via various cellular mechanisms. the vasodilators used were minoxidil sulfate (mnxs; 5 microm), brl-34915 (cromakalim, 0.1 microm), nicorandil (10 microm), pinacidil (1 microm), diazoxide (100 microm), sodium nitroprusside (10 ...19902292878
respiratory and metabolic responses in the horse during moderate and heavy exercise.thoroughbred horses were exercised to fatigue on a treadmill at 62% and 100% of their vo2max. hypoxemia occurred at the onset of exercise under both exercise conditions. this hypoxemia persisted to fatigue during the heavy exercise but progressively diminished as the exercise continued and had disappeared by the end of exercise at the lighter load. as a result of the hypoxemia the oxygen content of arterial blood during exercise at vo2max was 17% below its carrying capacity. however, under both ...19902293204
transdermal delivery of bioactive peptides: the effect of n-decylmethyl sulfoxide, ph, and inhibitors on enkephalin metabolism and transport.we investigated the effects of the nonionic surfactant, n-decylmethyl sulfoxide (ndms), ph, and inhibitors on the metabolism and the permeation of amino acids, dipeptides, and the pentapeptide enkephalin, through hairless mouse skin. an hplc gradient method was developed to identify the possible peptide and amino acid metabolites of leucine-enkephalin. ndms increased the permeability of all amino acids and peptides tested. at neural ph, the enzyme activity within the skin was such that no flux o ...19902293206
bubonic plague in a child presenting with fever and altered mental status.an 8-year-old navajo boy presented to the emergency department with fever and altered mental status. because the child lived in a plague-endemic area of the southwestern united states, antibiotics effective against yersinia pestis were administered rapidly. buboes were appreciated on physical examination, and the diagnosis of bubonic plague was confirmed by positive blood cultures. characteristics and treatment of yersinia pestis infection are discussed. the need for a high index of suspicion fo ...19902301801
genetic analysis of the yope region of yersinia spp.: identification of a novel conserved locus, yera, regulating yope expression.the yope gene of yersinia pseudotuberculosis was recently sequenced, and yope was identified as an indispensable virulence determinant when tested in a mouse model (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). in the study described here, the dna sequences of the yope genes of yersinia pestis ev76 and yersinia enterocolitica 8081 were determined and compared with that of the y. pseudotuberculosis gene. only two codons were found to differ, both leading to amino acid replacemen ...19902307658
synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32p-postlabeling analysis of human placental dna known to contain benzo[a]pyrene diol epoxide adducts.human placenta readily catalyzes the biotransformation of polycyclic aromatic hydrocarbons (pahs) and other carcinogens to reactive metabolites that can damage dna through formation of covalent adducts. placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures in humans. however, previous studies of human placental dna have yielded discrepant results with respect to pah-dna adducts. in order to resolve some of the issues surrounding these discr ...19902322996
production, characterization, and species specificity of monoclonal antibodies to mycobacterium avium complex protein antigens.the incidence of mycobacterium avium-mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated m. avium complex disease. in an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against m. avium. western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodi ...19902323822
long-term clearance of inhaled uo2 particles from the pulmonary region of the rat.this paper reports on an enriched uranium dioxide (uo2) mass clearance study undertaken with fischer-344 rats. the uo2 had a uranium (u) isotopic composition of 0.79% 234u, 92.8% 235u, 0.34% 236u and 6.06% 238u, by mass, with an alpha-particle activity of 1.91 bq micrograms-1. forty-six rats were exposed to an enriched uo2 aerosol that had an activity median particle aerodynamic diameter ranging from 2.7 to 3.2 microns. the rats were killed from 1 to 720 d post-inhalation (pi). the mass of enric ...19902323929
nucleotide sequence of porcine insulin-like growth factor. 1:5' untranslated region, exons 1 and 2 and mrna. 19902326169
plasmid content in yersinia pestis strains of different origin.plasmid content in 242 yersinia pestis strains from various natural plague foci of the u.s.s.r. and other countries was studied. of these strains, 172 (71%) were shown to carry three plasmids described previously of about 6, 45-50 and 60 mda, respectively. twenty strains (8%) from different foci harboured additional cryptic plasmids, most often of about 20 mda in size. plasmid ppst displayed considerable constancy of its molecular mass. on the contrary, size variations of pcad (45-49 mda) and, e ...19902328909
tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig.anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ...19902329918
a highly efficient electroporation system for transformation of yersinia.the various pathogenic yersinia species are not readily and efficiently transformed by classical methods. for this reason, the electroporation technique was applied for genetic transformation of these species. using optimal conditions, we were able to transform the six yersinia strains studied with the two most widely used groups of plasmids: psu2718 (a pacyc184 derivative) and pk19 (a puc19 derivative). only yersinia enterocolitica (y. e.) serotype 0:8 gave poor results (less than 5 x 10(2) tra ...19902332165
[plasmids of yersinia pseudotuberculosis].plasmids with the sizes of 5.7; 51; 70-77; and 120-130 kb were found in six strains among the ten strains collection of yersinia pseudotuberculosis. the restriction endonucleases analysis. southern-blot hybridization and physical maps construction were performed for the plasmids. the 70-77 kb plasmids were found to be analogous to the ca2(+)-dependence plasmid pyvo19 from yersinia pestis ev76. the difference between the plasmids of this type is in the insertions or deletions located on the simil ...19902333080
lipid soluble antioxidants preserve rabbit corneal cell function.the survival time of rabbit corneal endothelial cells in isolated perfused cornea is extended dramatically by the addition of oxygen free radical scavengers. lipid soluble antioxidants were found to be more effective than water soluble non-enzymatic antioxidants in extending the survival time. the ultrastructural appearance of endothelial cells treated with vitamin e was compared with controls. vitamin e treated endothelial cells retained normal appearance up to 12h, but without vitamin e, break ...19902335108
uveitis and immune responses in primates immunized with irbp-derived synthetic peptides.monkeys immunized with bovine irbp-derived synthetic peptides r4 (sequence 1158-1180) or r14 (1169-1191) developed eau which was detected by both clinical and histological examinations. the inflammation localized mainly in the choroid, with only minor changes being noticed in the adjacent retinal tissue. eau developed in only one of the two monkeys immunized with each of the peptides and the animals with disease also showed higher levels of cellular immunity toward the immunizing peptide than di ...19902335115
[glutathione and superoxide dismutase redox system in the development of toxic-infectious shock caused by yersinia pestis toxin].the changes in the glutathione-dependent and superoxide dismutase (sod) enzymatic activity in the rat lungs and liver tissues have been studied after the administration of plague murine toxin (ld100). it has been found out the early toxic effect in 1h in the lungs: 35% sod and glutathione peroxidase (tributyl hydroperoxide) (gp) decrease, 87% glutathione reductase (gr) increase along with two-hold ascent of ratio gr/glutathione-s-transferase (gt), gr/gps. the fundamental ratio gr/gt.gps rises in ...19902337651
[blood groups of great gerbils (rhombomys opimus licht.) as an indicator of population resistance to infections].three genetic systems of blood groups of great gerbils were studied to reveal that different resistance patterns exist in rodents toward yersinia pestis and salmonella typhimurium. extrapolation of the results obtained on relations of co-members of parasite systems contributes to understanding of some points of immunobiological mechanisms of dynamics of expizootic procedures. a hypothesis is proposed claiming that isoantigenic blood structure of the host is responsible for formation of populatio ...19902344947
[hepato-renal polycystic disease and liver hydatidosis (a rare association)].a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ...19902346686
excretion of yersinia spp. associated with consumption of pasteurized milk.yersinia enterocolitica biotype 1, serotype o.10k was isolated from 19 patients in the paediatric wards of a district general hospital over a period of 3 months. fifteen cases were patients on the medical ward. shortly afterwards, y. enterocolitica biotype 1, serotype o.6, 30 was isolated from a further 17 patients on this ward in 1 month. the same serotypes of y. enterocolitica were isolated from the pasteurized milk supplied to the ward. epidemiological evidence indicated that contaminated pas ...19902347379
an outbreak of foodborne botulism associated with contaminated hazelnut yoghurt.the largest recorded outbreak of foodborne botulism in the united kingdom occurred in june 1989. a total of 27 patients was affected; one patient died. twenty-five of the patients had eaten one brand of hazelnut yoghurt in the week before the onset of symptoms. this yoghurt contained hazelnut conserve sweetened with aspartame rather than sugar. clostridium botulinum type b toxin was detected in a blown can of hazelnut conserve, opened and unopened cartons of hazelnut yoghurt, and one faecal spec ...19902347382
[screening of plasmids in museum strains of yersinia pestis isolated from various natural foci].92 strains of yersinia pestis isolated from different natural foci and stored for 3-40 years in the museum of live cultures have been studied. the strains having three typical plasmids, their different combinations, plasmidless strains or the strains carrying nontypical plasmids with the molecular masses 9, 15, 55, 80, 90 and 150 md were found. the old museum strains are proposed to be used as a source of plasmids for the genetical research. the current control of plasmid contents in the museum ...19902362598
kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate.the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ...19902365688
[leptospirosis: a disease with multiorganic manifestations]. 19902366605
genotoxic effects of fluoride. 19902366812
[incidence of tuberculosis in the hunting tribe e-lun-chun in northeast china].the e-lun-chun, or ortochen, a national minority in north east china, consisted of 2,262 members in 1953. in the mid-17th century a russian invasion had displaced them from their original home in southward direction over the amur river towards the sin an lin mountain range. while coming into closer contact with neighbouring peoples, tuberculosis and other infectious diseases spread rapidly among the members of the tribe. under the japanese occupation (1932-1945) the incidence of pulmonary tuberc ...19902367435
[biophysical research on condylar temporomandibular joint growth].on the basis of results obtained from the study of mechanically induced potentials and pulsed 1h-nmr investigations of the condylar cartilage of the tmj in vitro, the hypothesis is advocated that the internal regulation of condylar growth takes place with the aid of bioelectric signals. reference in made to the importance of the studies for a future noninvasive diagnosis of condylar growth in the course of functional orthopedic treatment, using "dynamic" magnetic resonance imaging.19902373448
continuous intra arterial vasopressin infusion for control of typhoid hemorrhage.a 35 year old man presented with massive lower gastrointestinal hemorrhage due to typhoid enteritis. after confirming the site of bleeding on a selective superior mesenteric angiogram, a vasopressin infusion was given at the rate of 0.2-0.4 units/min initially, followed by tapering doses over 36 hours. cessation of bleeding was achieved immediately. the patients did not have any complications due to the procedure. continuous vasopressin infusion is an effective method for the treatment of uncont ...19902373534
ciprofloxacin penetration into human breast milk: a case report.the concentration of ciprofloxacin achieved in human breast milk following oral administration of a single 500-mg dose was investigated. a 24-year-old white woman, 17 days postpartum, was being treated for resolving acute renal failure. oral ciprofloxacin was prescribed for a suspected urinary tract infection. due to suspected pediatric toxicity and a lack of published information describing ciprofloxacin excretion in breast milk, the antibiotic was discontinued after one 500-mg dose. all breast ...19902375140
endotoxin-induced uveitis in the rat: a hypothesis for preferential involvement of the anterior uvea.endotoxin-induced uveitis (eiu) was produced in lewis rats by footpad injection of salmonella endotoxin. protein and cells were measured both in the aqueous humor and in the cerebrospinal fluid (csf) in order to examine if the inflammation was strictly limited to the anterior uvea. eiu was also induced in fischer and brown norway rats and the inflammation was compared among the three strains. although the function and structure of the choroid plexus is very similar to the ciliary body, no signs ...19902384004
[the phagocytic activity of peritoneal macrophages in relation to yersinia pestis with defective and complete fra genes].in vitro study of phagocytosis has shown that in guinea pigs fraction 1 is conducive to the ingestion of y. pestis by macrophages, to survival and proliferation of y. pestis cells in these macrophages, as well as to their specific transformation leading to their increased ingestive and bactericidal activity with respect to y. pestis. in mice the role of fraction 1 in phagocytosis has proved to be less significant.19902386004
[fluorescence of natural teeth and a composite]. 19902387172
experimental infection of ornithodoros spp. ticks (acari: argasidae) with yersinia pestis.ticks of the genus ornithodors feed on many rodent species with known roles in maintaining and amplifying plague (yersinia pestis) in nature. five species of ticks in this genus are shown to acquire the plague bacillus by feeding on infected hosts. most notably, 80% of o. hermsi wheeler, herms and meyer harbored y. pestis 1 mo after an infectious blood meal. subsequent transmission by infected ticks was not demonstrated. however, 1 yr after the infectious feeding, y. pestis dna was detected usin ...19902388251
use of elisa to reveal rodent infections in plague surveillance and control programmes.to assess its potential applications in plague surveillance and control programmes, an enzyme-linked immunosorbent assay (elisa) that captures the specific yersinia pestis f1 antigen was used to determine the amounts of this antigen present in samples of blood and spleens from laboratory rats with acute plague, in the buboes of rats with chronic plague, and in tissues from the carcasses of rats that had died from plague. in rats with acute plague, bacteria and f1 antigen were detected in samples ...19902393980
hydrophobic surfaces of tubulin probed by time-resolved and steady-state fluorescence of nile red.binding of nile red to tubulin enhances and blue-shifts fluorescence emission to about 623 nm with a "shoulder" around 665 nm. binding is reversible and saturable with an apparent kd of approximately 0.6 microm. nile red does not alter tubulin polymerization, and polymerization in 2-(n-morpholino)ethanesulfonic acid (mes) buffer does not alter the spectrum of the nile red-tubulin complex. in contrast, polymerization in glutamate buffer results in a red shift, reduction of intensity, and a decrea ...19902394705
tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners.the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ...19902394748
ambulatory activity in streptozotocin-induced diabetic rats.this study was undertaken to investigate changes in ambulatory and drinking behavior, using the gunma university-type special apparatus for continuous and direct measurement of ambulation and drinking in streptozotocin-induced diabetic rats (stz, 60 mg/kg). ambulatory activity of diabetic rats was significantly less than that of control rats during the dark phase but not during the light phase. ambulatory activity of diabetic rats was also significantly less from 0900 to 1000 hr, but significant ...19902395920
[toxicological considerations in the evaluation of veterinary drugs].interactions between veterinary pharmacotherapy, toxicology of residues, prevention of residues of veterinary drugs and the evaluation of veterinary drug files are discussed on the basis of a number of examples. sulphadimidine is used to treat atrophic rhinitis in medicated feeds which do not benefit the animal but are the cause of persistent sulphonamide residues in feed mills and husbandry. carbadox is a potentially effective prophylactic feed additive for the prevention of swine dysentery, bu ...19902396245
[the potentiating action of artificial immunodeficiency on the development of amyloidosis in plague in great gerbils].cyclophosphamide injection causes amyloidosis development in the spleen of great gerbils both intact and plague-infected after immunization. the amyloid deposits are observed irrespective of acquired antiplague resistance level. alterations observed may be considered as acquired (secondary) aa-amyloidosis. results indicate the significance of immunodepression in amyloidogenesis and a possibility to use the cyclophosphamide-treated great gerbils as a convenient experimental model of amyloidosis.19902396918
trisomy 14q in myelodysplastic syndromes. 19902397464
yopm inhibits platelet aggregation and is necessary for virulence of yersinia pestis in mice.in yersinia pestis kim there are 11 yops (yersinial outer membrane proteins) encoded by the low-ca2+ response virulence plasmid pcd1. only yopm and yopn are found in easily detectable amounts in the culture medium. in our previous work, we characterized the yopm gene. in the present study, we constructed a yopm- mutant to elucidate the role of yopm in the virulence of y. pestis. a laczya sequence was inserted 126 base pairs downstream from the start codon of the yopm gene in pcd1. the yopm- muta ...19902401564
a projection from the deep layers of the entorhinal area to the hippocampal formation in the rat brain.the methods of retrograde fluorescent tracing and anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) were used to demonstrate the existence of projections from layers iv and vi of the entorhinal area to the hippocampal formation in the rat brain. these two layers of the medial and lateral entorhinal area innervate the molecular layer of ammon's horn and the area dentata. in the area dentata the projection from layer iv follows that of the perforant path, while that fr ...19852409482
a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin.in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative.19852412878
monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species.the highly reproducible histochemical localization of glial fibrillary acidic protein (gfap)+ qualifies it as an important marker of astrocytes in both research and clinical applications. the primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to gfap-specific epitopes of wide species distribution. we report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitope ...19852413405
[vital staining of the conjunctiva for prevention of epithelial implantation cysts].selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ...19852415747
[requirements and standardization of the conditions for carrying out the microfluorometric test]. 19852415765
[escherichia coli neuraminidase].the intracellular form of neuraminidase has been detected in e. coli and proteus vulgaris. neuraminidase has been isolated from e. coli hb 101 cells and purified 118-fold. some physico-chemical properties of this enzyme have been studied.19862422854
the response of the lung to foreign compounds that produce free radicals. 19862423026
characterization of receptors regulating secretory function in the fundic mucosa.a model for a present view of the major pathways and receptors mediating function in the canine fundic mucosa is depicted in figure 1. gastrin has direct actions on the parietal cell and on the somatostatin cell; action on the parietal cell, but not somatostatin cell, is potentiated by histamine. in contrast, gastrin action on the somatostatin cell is potentiated by beta-adrenergic agonists. the potency of h2 blockers against gastrin may reflect blockage by these inhibitors of the stimulatory (p ...19862423027
separation and characterization of four different amylases of entamoeba histolytica. ii. characterization of amylases.purified e. histolytica amylases iii to vi were characterized by their hydrolytic behaviour towards 4-nitrophenyl alpha-malto-oligosaccharides, malto-oligosaccharides, amylose, amylopectin, glycogen and y-cyclodextrin. the influence of specific inhibitors on the amylase activity of e. histolytica was examined and compared with typical alpha- and beta-amylases. amylases iii and iv showed alpha-glucosidase and glucosyltransferase activity by cleaving terminal non-reducing glucose from pnpg1 (iii, ...19862423098
the wellcome trust lecture. genes for antigens of plasmodium falciparum. 19862423947
estimation of gene expression in heterocysts of anabaena variabilis by using dna-rna hybridization.in the filamentous cyanobacterium anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. we used two different types of dna-excess rna hybridization techniques to estimate the number of genes expressed in recently differentiated, mature heterocysts. in the first, rna and dna were incubated in a phosphate buffer at 60 degrees c, and the hybrids were separated from the unhybridized material by hydroxylapatite ...19862427500
amelioration of postischemic acute renal failure by prostacyclin analogue (iloprost): long-term studies with chronically instrumented conscious dogs.the potential therapeutic value of the chemically stable carbacyclin analogue iloprost on the course of postischemic acute renal failure was studied in six conscious chronically instrumented dogs and compared with five controls. immediately after temporary ischemia (180-min cessation of blood flow by inflation of a pneumatic cuff), the investigational group pc received a continuous intraaortal infusion of iloprost (50 ng x min-1 x kg-1) over a period of seven days, whereas the control group c re ...19862427819
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...19862430056
aminergic neurotransmitter and water content changes in rats after transient forebrain ischemia.we have studied changes of cerebral monoamine metabolism and water content, during recirculation following global transient ischemia (20 min) using the four-vessel occlusion model in rats. levels of monoamines and their metabolites were determined in cortex, striatum, hippocampus, and hypothalamus. water content was evaluated by weight and by the analysis of t1 and t2 relaxation times in 1h-nuclear magnetic resonance. norepinephine levels decreased; 3,4-dihydroxyphenylethylamine, 3,4-dihydroxyph ...19862430056
the origin of cells in the glomerular crescent investigated by the use of monoclonal antibodies.a study was made of the cells forming the crescents in human crescentic glomerulonephritis. the investigation was performed using a panel of antibodies with immunoperoxidase techniques in formalin fixed, paraffin embedded renal biopsy material. some of the cells of glomerular crescents were found to contain cytokeratin intermediate filaments, as did some of the cells of the normal parietal epithelium of bowman's capsule. leucocytes were also found in crescents, often in the outer part, and their ...19862430880
[use of immunospecific components labelled with beta-lactamase and horseradish peroxidase for immunoenzyme analysis].a system for enzyme immunoassay with the use of beta-lactamase as an enzyme marker is described. similar sensitivity of the enzyme immunoassays with the use of beta-lactamase and horse-raddish peroxidase as enzyme markers was shown. the data on decrease of the antigen binding capacity of the antibodies in the conjugates with beta-lactamase are presented. it is suggested that such a decrease may be associated with glutaric dialdehyde conjugation. the use of the schemes for enzyme immunoassay with ...19862434023
assessment of ischemia reperfusion injury in skeletal muscle by macromolecular clearance.qualitative changes in skeletal muscle injury after ischemia are well known; however, quantitative assessments have not been well documented. we have determined microvascular permeability changes by measuring the clearance of fluorescein-labeled dextran of mw 150,000 (fitc-dextran-150). the cremaster muscle of anesthetized rats was fashioned as a single layer, splayed on a lucite chamber and suffused with bicarbonate buffer solution at 35 degrees c. clearance is the product of suffusion rate tim ...19872438477
antiviral treatment of chronic type b hepatitis. 19872441635
discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha.the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ...19872441999
[levels of fraction i and vw antigens in cultures of the plague microbe grown on yeast-casein, yeast-hottinger broth and yeast-sunflower oil cake media].the content of fraction 1 and vw-antigens in y. pestis cultures grown in different media (yeast-casein medium, yeast medium with hottinger digest, and yeast medium with sunflower-seed protein) was studied over the course of their growth by means of the antibody neutralization and microprecipitation in agar tests. the media under study were not inferior to the casein sulfuric hydrolysate-based medium used for control in their capacity for ensuring the synthesis of vw-antigens. the maximum accumul ...19872442925
translation framing code and frame-monitoring mechanism as suggested by the analysis of mrna and 16 s rrna nucleotide sequences.protein coding sequences carry an additional message in the form of a universal three-base periodical pattern (g-non-g-n)n, which is expressed as a strong preference for guanines in the first positions of the codons in mrna and lack of guanines in the second positions. this periodicity appears immediately after the initiation codon and is maintained along the mrna as far as the termination triplet, where it disappears abruptly. known cases of ribosome slippage during translation (leaky frameshif ...19872443708
a new look at uterine muscle contraction.recent progress in our understanding of uterine smooth muscle contraction is reviewed. we no longer believe that actin-myosin interaction in the myometrium occurs through activation of the thin filament; but it is triggered by calcium-dependent phosphorylation of myosin in the thick filament. calcium is now thought to originate from both extracellular and intracellular sources. calcium can enter the cell through either a voltage- or a hormone-controlled calcium channel. the intracellular source ...19872446500
heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells.cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ...19882446745
galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons.galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ...19872446770
rapid staining of campylobacter pyloridis. 19872447004
the golgi methods applied to the human and rat subfornical organ. 19872448966
[development of an immunoenzyme method for determining yersinia pestis 43000 antigen]. 19882457734
the effect of unilateral naris occlusion on cell dynamics in the developing rat olfactory epithelium.the effect of unilateral naris occlusion on the cellular dynamics of developing olfactory epithelium was investigated in postnatal rats. nares of rat pups, at 1, 5, and 10 d postnatally, were cauterized; after a 30 d survival period, the olfactory mucosa was examined histologically, and quantitative estimates were made of total number of receptor neurons (together with basal cells), supporting cells, and epithelial thickness. in each group, there were significant differences between occluded and ...19882459323
[an immunoenzyme monoclonal test system for detecting the causative agents of plague]. 19882462124
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