| secretion of macrophage neutral proteinase is enhanced by colchicine. | we have studied the effect of colchicine and related compounds on secretion of enzymes by thioglycollated-elicited mouse peritoneal macrophages in culture. colchicine stimulated secretion of inducible neutral proteinase activities of elastase (ec 3.4.21.11), collagenase (ec 3.4.24.3), gelatinase (pepsin b; ec 3.4.23.2), and azocaseinase 2- to 6-fold for a period of several days, but inhibited the production and release of lysozyme (mucopeptide n-acetylmuramoylhydrolase; ec 3.2.1.17), a noninduci ... | 1976 | 176659 |
| cellular fatty acids during fowlpox virus infection of three different host systems. | | 1976 | 176806 |
| infectivity of visna virus dna. | | 1976 | 176812 |
| [glycogen content and the activity of glycogen synthetase in the brain under the effect of cyclic amp and psychotropic drugs]. | | 1975 | 176847 |
| steroid-binding proteins in carcinoma of the human male breast. | receptor sites for a group of four steroid hormones were estimated by competitive binding assay of a biopsy specimen of carcinoma from a male breast. the results indicate that the tissue contained a significant number of binding sites for 17beta estradiol, and probably for dihydrotestosterone and progesterone, but not for testosterone. these preliminary data suggest that, as in the female, certain mammary carcinomas in the male may be under the influence of steroid hormones. correlation of such ... | 1976 | 176884 |
| carcinogenic activity of quinoline on rat liver. | the effects of prolonged p.o. administration of quinoline or 2-chloroquinoline on rat liver were examined histologically. hepatocellular carcinomas and hemangioendotheliomas were observed in the livers of rats fed a basal diet containing 0.05, 0.10, or 0.25% quinoline for about 16 to 40 weeks. however, no neoplastic changes were seen in the livers of rats fed a basal diet containing 0.05, 0.10, or 0.25% 2-chloroquinoline for 40 weeks. in groups that received low concentrations of quinoline, the ... | 1976 | 177193 |
| [changes in the level of acth in the fetal hypophysis. a study in the rat toward the end of gestation]. | the isolated adrenal cell method for bioassay of acth was employed in order to determine the acth content of the hypophysis of the rat foetus, in late pregnancy. the acth concentration decreases between 17 days 8 h and 17 days 15 h, then it increases regularly until the term. this evolution is correlated with the change in the fluorescence intensity of the corticotrophs and with the modification of the adrenocortical activity. | 1976 | 177226 |
| the role of serum nonsuppressible insulin-like activity (nsila) in compensatory renal growth. | the nonsuppressible insulin-like activity (nsila) of serum is defined as the insulin-like activity measured in insulin bioassays that is not suppressed by addition of insulin antiserum. the major part of serum nsila is attributable to a 90,000 mol wt trace protein which has been recently purified. this study was designed to evaluate the role of serum nsila in compensatory renal growth (crg). | 1976 | 177337 |
| coxsackievirus and adenovirus infection. association with acute febrile and juvenile rheumatoid arthritis. | the prearthritic manifestations of juvenile rheumatoid arthritis in a 16-year-old boy were associated with a rise in coxsackievirus b3 and a9 neutralizing antibody titers from 1:16 to larger than or equal to 1:512, and 1:64 to 1:512, respectively. recurrent polyarthritis followed and has persisted for three years. adenovirus 7 was isolated from the pericardial fluid of a 9-year-old girl in whom juvenile rheumatoid arthritis then developed. of 11 patients with acute, nonspecific, febrile arthriti ... | 1976 | 177792 |
| acidic steroid metabolites: evidence for the excretion of c-21-carboxylic acid metabolites of progesterone in rabbit urine. | | 1976 | 177811 |
| investigations on the turnover of adrenocortical mitochondria. iv. a stereological study of the effect of chronic treatment with acth on the size and number of rat zona reticularis mitochondria. | the effects of chronic administration of of acth (up to 36 consecutive days) on the mitochondria of the zona reticularis of the rat adrenal cortex were investigated by stereologic techniques. it was found that acth induces two phases of hypertrophy of mitochondria alternating with two proliferative stages, which are associated with a significant decrease in the average volume of the organelles. it is suggested that, as in the zona fasciculata, acth controls the processes of growth and division o ... | 1976 | 178447 |
| pituitary cyclic amp production and mechanism of luteinizing hormone release. | | 1975 | 178535 |
| generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin g. | extent of o-2-release and chemiluminescence, attributed to singlet oxygen, has been compared in human monocytes and neutrophils during phagocytosis, stimulation by the surface-active agent phorbol myristate acetate, or contact with aggregated igg in a model of immune complex disease. monocytes generated o-2-and chemiluminescence with each of the three stimuli, although values were significantly less than those of neutrophils from the same individuals. lymphocytes had no significant activity in e ... | 1976 | 178824 |
| nad+-malic enzyme. regulatory properties of the enzyme from ascaris suum. | the regulatory properties of the nad-dependent malic enzyme from the mitochondria of ascaris suum have been studied. the malate saturation curve exhibits sigmoidicity and the degree of this sigmoidicity increases as the ph is increased. fumarate was the only compound tested that stimulated the enzyme activity, whereas oxalacetate was the most powerful inhibitor. activation by low levels of fumarate was found to be competitive with malate. it is proposed that this stimulation has physiological si ... | 1976 | 180002 |
| herpes simplex virus specified deoxypyrimidine kinase and the uptake of exogenous nucleosides by infected cells. | herpes simplex virus can confer to thymidine kinaseless cells the ability to incorporate exogenously supplied thymidine into acid precipitable material. however no incorporation of exogenously supplied deoxycytidine into acid precipitable material can be detected after infection of deoxycytidine kinaseless cells by herpes simplex virus. this failure to incorporate exogenous deoxycytidine is not due to the failure of the deoxycytidine phosphorylating activity of the virus induced deoxycytidine ki ... | 1976 | 180246 |
| serum testosterone and dihydrotestosterone in patients with trophoblastic disease. | serum testosterone (t) and dihydrotestosterone (dht) were measured by radioimmunoassay in 14 patients with unaborted hydatidiform mole and in 16 patients with normal pregnancy of similar gestational age. serum human chorionic gonadotropin (hcg) was measured by the radioreceptor assay in patients with hydatidiform mole. serum t ranged from 0.27 to 5.39 ng/ml with a mean +/- se of 2.21 +/- 0.45 ng/ml in patients with hydatidiform mole mole and from 0.20 to 2.40 ng/ml with a mean +/- se of 0.80 +/- ... | 1976 | 180465 |
| regulation of pyruvate dehydrogenase in rat heart. mechanism of regulation of proportions of dephosphorylated and phosphorylated enzyme by oxidation of fatty acids and ketone bodies and of effects of diabetes: role of coenzyme a, acetyl-coenzyme a and reduced and oxidized nicotinamide-adenine dinucleotide. | the proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart was decreased by alloxan-diabetes or by perfusion with media containing acetate, n-octanoate or palmitate. the total activity of the dehydrogenase was unchanged. 2. pyruvate (5 or 25mm) or dichloroacetate (1mm) increased the proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart, presumably by inhibiting the pyruvate dehydrogenase kinase reaction. alloxan-diabetes markedly decre ... | 1976 | 180974 |
| on the utilization of l-glutamine by glutamate dehydrogenase. | | 1976 | 180990 |
| inhibition of estrogen-receptor-dna interaction by intercalating drugs. | ethidium bromide, an intercalating drug, was shown to inhibit the in vitro dna binding of the uterine estradiol-receptor complex. the inhibition was reversible, dose dependent, complete for total saturation of dna intercalating sites by the dye, and proportional to the extent of intercalated drug. the binding of the receptor to phosphocellulose and poly(adenylic acid)-cellulose was not decreased by this drug. similar inhibition was also obtained with 9-hydroxyellipticine. denatured dna was more ... | 1976 | 182199 |
| testosterone-binding protein in reproductive tracts of fetal rats. | testosterone-binding proteins may mediate the induction of wolffian duct differentiation by testicular testosterone. the presence of such protein(s) was sought in reproductive tracts of 14.5-21.5-day-old fetal rats. supernatant fractions (127,000 x g) were equilibrated with [3h]t) +/- radioinert testosterone in tris - hcl:edta buffer, ph 7.4, (approximately 0.1 mg protein/0.5 ml) at 4 c for 16 hours. bound and free (3h)t were separated by charcoaldextran adsorption or sephadex g-100 gel filtrati ... | 1976 | 182453 |
| in vitro behavior of human intestinal mucosa. the influence of acetyl choline on ion transport. | the possibility that the autonomic nervous system may influence the function of intestinal mucosa was investigated by assessing the effect of acetyl choline on ion transport in human intestine. isolated pieces of stripped ileal mucosa were mounted in perspex flux-chambers and bathed in isotonic glucose ringer's solution. acetyl choline caused a rise in mean potential difference (8.8-12.3 mv, p less than 0.002) and short circuit current (287.7-417.2 mua-cm-2, p less than 0.01) (n = 12), observabl ... | 1976 | 182722 |
| epstein-barr virus latency and bacteriophage p1 lysogeny-possible analogies. | | 1975 | 183687 |
| regulation of carbohydrate permeases and adenylate cyclase in escherichia coli. studies with mutant strains in which enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system is thermolabile. | carbohydrate uptake and cyclic adenosine 3':5'-monophosphate (cyclic amp) synthesis were studied employing mutant strains of escherichia coli in which enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system was heat-labile. partial loss of enzyme i activity, which resulted from incubation of cells at the nonpermissive temperature, depressed the rate and extent of methyl alpha-glucoside uptake. temperature inactivation of enzyme i also rendered cyclic amp synthesis and the uptake of s ... | 1976 | 184083 |
| cell coat in tumor cells -- effects of trypsin and edta: a biochemical and morphological study. | the effects of trypsin and edta on the cell coat of ascites tumor cells were studied by means of biochemical and electron microscopy techniques. edta seems to release, by chelation of the ca2+-bridges, an outer layer of glycoproteins of presumably exogenous origin. on the contrary, trypsin produces a deeper enzymic cleavage which appears to affect the structural integrity of the bilayered cell membrane. the significance of the cell leakage in tumor cells and the effect of edta on the modificatio ... | 1976 | 185564 |
| ultrastructure of lipopolysaccharides of yersinia enterocolitica, salmonella typhimurium and escherichia coli. | the fine structure of isolated lipopolysaccharides (lps) from the rough and smooth form of an yersinia enterocolitica strain (ye 75 r/ye 75 s) and from smooth forms of salmonella typhimurium (s 1010) and escherichia coli (essen) were examined electron-microscopically by negative staining. partial denaturation of lps in tris-buffer with acid and/or polymyxin b treatment revealed a common structure of strandlike lps. electron-microscopically, lps-strands were found to consist of two identical sub- ... | 1976 | 185850 |
| uridine--cytidine kinase from foetal and adult rat liver. purification and study of some properties. | partial purification of uridine--cytidine kinase (ec 2.7.1.48) from foetal rat liver by chromatography on deae-cellulose gives two active fractions. the first in order of elution was identified as a form specific for foetal liver. it was purified 300-fold. the second fraction was common to foetal, and adult rat liver and spleen and was purified 20-fold. the foetal fraction of the enzyme was found to be heat-sensitive and protected against inactivation by po34- anions. the two isolated forms have ... | 1976 | 186262 |
| effect of cerebral injury on cerebrospinal fluid cyclic amp concentration. | cerbrospinal fluid (csf) cyclic adenosine-3', 5'-monophosphate (camp) was measured in rabbits after experimental brain injury as well as in patients with cerebral contusion and cerebral concussion. in rabbits a marked elevation lasting for two weeks was observed. from the third week onwards after the injury the csf camp concentration was lower than the basal level before the injury. dexamethasone partly inhibited the elevation. ethanol treatment decreased the csf camp values. in man, the csf cam ... | 1976 | 186266 |
| effect of epinephrine on the oxidative desaturation of fatty acids in the rat. | the effect of epinephrine on the oxidative desaturation of fatty acids by liver microsomal preparations of rats has been studied. administration of epinephrine (1 mg/kg body weight) produced a significant decrease in desaturation of [l-14c]=linoleic acid to gamma-linolenic acid and of [l-14c]alpha-linolenic acid to actadeca-6,9,12,15-tetraenoic acid 12 hr after the infection. lower doses produced a lesser effect on the delta6-desaturation activity. epinephrine administration modified the v max o ... | 1976 | 186549 |
| [blood lipoprotein transfers in the normal arterial wall. study auto-historadiography in optic microscopy]. | in order to study the mecanisms of serum lipoproteins transfers and binding sites in arterial wall, auto-historadiographies in optical microscopy were realised with rats aortas. animals received infusion of in vivo labelled on the cholesterol and purified serum lipoproteins. the results confirmed the serum lipoproteins transfers in arterial wall and slow that the inflow is all the more quicker and higher as the molecule is smaller. the very particular aspect of these transfers rises several hypo ... | 1975 | 186745 |
| transfer of parental immunity to infectious laryngotracheitis in chicks. | the transfer of parental immunity to infectious laryngotracheitis was appraised by measuring serum antibody levels in 150 chicks from the day of hatch up to five weeks. the breeder flock which had received primary vaccination at eight weeks and a booster at 20 weeks transferred high antibody levels which fell markedly within two weeks and remained constant thereafter. chicks whose parents were vaccinated at 20 weeks only, had low antibody levels throughout. these low levels, in either group of c ... | 1976 | 187298 |
| interrelationships between the central nervous system and patterns of adrenocorticotropic secretion following acute exposure to severe environmental conditions. | | 1976 | 187557 |
| the application of rapid kinetic techniques to the transport of thymidine and 3-o-methylglucose into mammalian cells in suspension culture. | techniques are described by which the transport of nutrients into mammalian cells in suspension can be measured at intervals of 1.5 seconds. by application of these techniques, the existence of a saturable (km = 85 mum), non-concentrative, transport system for thymidine was demonstrated in novikoff rat hepatoma cells depleted of atp. at concentrations of thymidine less than the km, this system operated at velocities sufficient to nearly completely equilibrate intra- and extra-cellular thymidine ... | 1976 | 188833 |
| glucocorticoid and thyroid hormone-binding to nuclear ribonucleoprotein particles. | | 1977 | 193730 |
| experimental evaluation of paracetamol antidotes. | | 1976 | 193746 |
| relationship between transcerebral passage of tumor cells and brain metastasis. | quantitative study was carried out on transcerebral passage of tumor cells using yoshida sarcoma and 6 strains of rat ascites hepatoma. the counting was done every second for 40 sec. in relation to the results obtained, histological study was made on the cerebral metastasis of these tumor cells. metastatic foci in the brain parenchyma, except the meninges and choroid plexus, were formed by island-forming strains (ah-130, ah-272, and ah-7971) which showed comparatively low passage rate and not be ... | 1977 | 193754 |
| purification and subunit composition of guanosine 3':5'-monophosphate-dependent protein kinase from bovine lung. | the guanosine 3':5'-monophosphate-dependent protein kinase from bovine lung was purified to apparent homogeneity by affinity chromography using 8-2-aminoethylthio-cgmp coupled to sepharose 4b. the kinase activity was purified approximately 6000-fold with an overall recovery of approximately 20%. the product isolated by affinity chromatography contained both cgmp-binding and cgmp-dependent histone kinase activity, indicating that the enzyme was not dissociated into regulatory and catalytic compon ... | 1977 | 193862 |
| variables affecting viral plaque formation in microculture plaque assays using homologous antibody in a liquid overlay. | a liquid antibody microculture plaque assay and the variables that govern its effectiveness are described. the assay is based on the principle that low concentrations of homologous antibody can inhibit secondary plaque formation without inhibiting formation of primary plaques. thus, clear plaques that followed a linear dose response were produced. the assay was found to be more rapid, less cumbersome, and less expensive than assays using agar overlays and larger tissue culture plates. it was rep ... | 1977 | 194918 |
| splenic influence of t cell function: the immunobiology of the inbred hereditarily asplenic mouse. | | 1977 | 194980 |
| reduction of postperfusion cytomegalovirus-infections following the use of leukocyte depleted blood. | after extracorporeal perfusion some patients develop evidence of cytomegalovirus (cmv) infection. it is proposed that antigenically stimulated donor and recipient leukocytes may divide and activate latent cmv associated with leukocytes. to test this hypothesis, alternate patients were perfused with leukocyte-depleted or whole blood (controls). four of six controls who were cmv antibody negative preoperatively seroconverted after perfusion. virus was recovered from the blood of three and from the ... | 1977 | 195372 |
| substrate positions and induced-fit in crystalline adenylate kinase. | | 1977 | 198550 |
| rna: dna ratios in human tumors. | | 1977 | 198554 |
| testicular binding of 125i-hcg in developing estrogenized and androgenized rats. | the present work describes changes seen in the binding of 125i-hcg by testis homogenates of rats injected at the age of three days with 400 microgram estradiol-17beta-dipropionate, or 250 microgram testosterone propionate. estrogenized and androgenized male rats showed a marked decrease of gonadotropin binding in testis at the 30th, 45th and 60th postnatal day. these results show that delayed sexual maturation of male rats treated with estrogen and androgen in the neonatal period is also related ... | 1977 | 199424 |
| the in vitro and in vivo activity of taurolin against anaerobic pathogenic organisms. | an antibacterial agent, taurolin, formed by the combination of 2 molecules of taurine with 3 molecules of formaldehyde has been tested against anaerobes in vitro and in vivo. in vitro, taurolin is highly active against bacteroides species and anaerobic streptococci as measured by the minimum inhibitory concentration. in vivo, small dosages of taurolin given intraperitoneally increase the mortality in mice with bacteroides peritonitis. however, by increasing the dosage of taurolin, survival is pr ... | 1977 | 201040 |
| depletion of replenishment of cellular cyclic adenosine monophosphate in hemorrhagic shock. | hemorrhagic shock in rats was produced by bleeding them to a mean arterial blood pressure of 40 millimeters of mercury, which was maintained for two hours. after sacrifice, small pieces of liver, kidney, muscle and brain were quickly removed and frozen in liquid nitrogen. a protein-free extract of tissues was prepared, and cyclic adenosine monophosphate was measured by the radioimmunoassay procedure. analysis os liver, kidney, muscle and brain showed that there were significant reductions in cyc ... | 1977 | 201041 |
| an italian case of nasopharyngeal carcinoma correlated with epstein-barr virus specific antigens. | an italian case of nasopharyngeal carcinoma was studied clinically; the immunologic study showed the correlation with the epstein-barr virus specific antigen. | 1977 | 201063 |
| [content of free amino acids in rabbit tissues as affected by adrenalectomy and administration of hydrocortisone and acth]. | adrenalectomy is accompanied by a decreased content of free amino acids in muscules and blood plasma by an increased content of certain amino acids in the liver. a single injection of hydrocortisone to adrenalectomized animals increases the content of amino acids in muscles and lowers their total content in the liver; a single injection of acth lowers the total content of amino acids in the liver and blood plasma "substitutive therapy" by hydrocortisone essentially increases the content of amino ... | 1977 | 201065 |
| [fine structure of cholangiofibromas induced in the rat by n-nitrosomorpholine (author's transl)]. | | 1977 | 201084 |
| [reproduction, morphogenesis and immunobiological properties of aujeszky's disease virus commercially cultured in a suspension of poultry embryo explants]. | the results of the study of reproduction, morphogenesis, and immunogenic properties of aujeszky's disease virus (adv) cultivated on an industrial scale in chick, duck and quail embryo explant suspensions are presented. a method for production of vaccine and virulent adv strains with the activity of at least 7.5 log tcd50/ml in tanks of 5, 50, and 100 1 capacity was developed. a relationship between virion morphology and duration of cultivation was established. for adv cultivated for 70 hours, a ... | 1977 | 201102 |
| [action mechanism of respiratory inhibition by systemic fungicides of the carboxin group. action of oxathiin derevatives and analogs on nonphosphorylating submitochondrial particles from ox heart as well as trametes versicolor and trichoderma viride]. | | 1977 | 201109 |
| cell culture propagation of porcine rotavirus (reovirus-like agent). | two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ... | 1977 | 201198 |
| possibility of eb virus preferentially transforming a subpopulation of human b lymphocytes. | | 1977 | 201868 |
| studies on nad+ permeability in intact mitochondria from rabbit reticulocytes. | functionally intact mitochondria from rabbit reticulocytes are characterized by a low nad+ level after the preparation (0.29 nmoles nad+ + nadh/mg protein). they are apparently impermeable for nadh and exhibit a slow net uptake of nad+. from the increase of o2-uptake in state 3 and the increase of nadh concentration in state 4 of respiration after the addition of nad+ we concluded that 3--10 min are necessary for the saturation with nad+ at 23 degrees c. 2mm nad+ extramitochondrially are not suf ... | 1977 | 202125 |
| functional studies on crosslinked bovine cytochrome c oxidase. | | 1978 | 202495 |
| cellular immune responsiveness of breast cancer patients to parsi human milk particles & mammary tumor virus: lymphocyte blastogenesis. | | 1977 | 202562 |
| natural cell-mediated cytotoxicity in rats. iii. effects of immunopharmacologic treatments on natural reactivity and on reactivity augmented by polyinosinic-polycytidylic acid. | treatment of rats with high doses of hydrocortisone, x-irradiation, or cyclophosphamide had a suppressive effect on natural cytotoxicity in vivo. however, when rats were given poly i:c after any of these agents, the levels of nk activity were similar to those in normal rats which had been given poly i:c alone. to explain these findings, we have postulated that a population of pre-nk cells, resistant to hydrocortisone, cyclophosphamide and x-irradiation was induced by poly i:c to become cytotoxic ... | 1978 | 203549 |
| retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity. | a sepharose 4b chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques. | 1978 | 205680 |
| kinetic studies on the reactions catalyzed by chorismate mutase-prephenate dehydrogenase from aerobacter aerogenes. | steady-state kinetic techniques have been used to investigate each of the reactions catalyzed by the bifunctional enzyme, chorismate mutase-prephenate dehydrogenase, from aerobacter aerogenes. the results of steady-state velocity studies in the absence of products, as well as product and dead-end inhibition studies, suggest that the prephenate dehydrogenase reaction conforms to a rapid equilibrium random mechanism which involes the formation of two dead-end complexes, viz, enzyme-nadh-prephenate ... | 1978 | 206281 |
| thermotropic changes in the surface structure of lipoprotein b from human-plasma low-density lipoproteins. a spin-label study. | | 1978 | 206433 |
| energy metabolism of spermatozoa. vii. interactions between lactate, pyruvate and malate as oxidative substrates for rabbit sperm mitochondria. | | 1978 | 207366 |
| neonatal haematological abnormalities associated with maternal disease. | | 1978 | 207479 |
| [intestinal yersiniosis: 25 cases of infections with yersinia pseudotuberculosis and yersinia enterocolitica (author's transl)]. | yersinia infections in 16 adults and 9 children are reported. 15 cases were caused by yersinia pseudotuberculosis, 8 of them were proved by serological findings. in 4 cases the infection by yersinia pseudotuberculosis was likely, in 3 other cases possible only. real infections caused by serotype iv are demonstrated too. furthermore there were 10 cases caused by yersinia enterocolitica, 3 of them were proved by bacterial, the other ones by serological findings. both germs caused identical symptom ... | 1978 | 207960 |
| tumor imaging with x-rays using macrophage uptake of radiopaque fluorocarbon emulsions. | radiopaque fluorocarbon (rfc) emulsions were prepared with small particle size and high concentration of the fluorocarbon. when rfc emulsions were injected intravenously in hamsters, rats, and mice with eight types of malignant tumors, the tumors became radiopaque, except in the mice with spontaneous teratoma of the ovaries. tumors as small as 3 to 4 mm could be defined radiographically using routine x-ray techniques. the tumors remained radiopaque for days to weeks after injection. light and el ... | 1978 | 208188 |
| differential effects of isolated lipoproteins from normal and hypercholesterolemic rhesus monkeys on cholesterol esterification and accumulation in arterial smooth muscle cells in culture. | whole serum obtained from hypercholesterolemic rhesus monkeys was found to stimulate cholesterol esterification and cholesteryl ester accumulation in rhesus monkey arterial smooth muscle cells in culture to a significantly greater extent than normocholesterolemic serum. this was true even when the cholesterol concentration of the culture medium was equalized. isolation and characterzation of the low density lipoproteins (ldl) from rhesus monkeys indicated that the ldl from hypercholesterolemic a ... | 1978 | 208631 |
| characterization of the to strains of theiler's mouse encephalomyelitis viruses. | theiler's mouse encephalomyelitis virus isolates from the central nervous systems of spontaneously paralyzed mice and stools of asymptomatic mice resemble theiler's original virus isolates. in this study four such strains were adapted by blind subpassage to replicate and to produce cytopathic effect in cell culture. these viruses were then found to be closely related to each other and to gdvii virus by cross-neutralization and to form small plaques. bovine serum was found to contain cross-reacti ... | 1978 | 208981 |
| effect of starvation on gluconeogenic enzymes in the small intestine of rats. | | 1978 | 210118 |
| [attempts to demonstrate and identify avian adenoviruses by an immunofluorescence method]. | a specific conjugate of a hyperimmune serum, obtained from rabbits with the use of the reference phelps-celo strain, was employed in a direct immunofluorescence method to investigate more than 320 lamelles with cell cultures infected with 4 field celo virus strains and the reference phelps-celo strain. it is stated this method is specific and prompt for the early diagnosis and identification of adenoviruses (celo viruses). | 1978 | 210562 |
| sensitivity of yersinia pseudotuberculosis and yersinia enterocolitica strains to a 6-beta-amidino penicillanic acid derivative. | | 1978 | 211112 |
| association of yersinia enterocolitica with the manufacture of cheese and occurrence in pasteurized milk. | raw milk in southern ontario frequently contains yersinia enterocolitica. the potential for transmission of this organism by cheese manufactured from unpasteurized milk was evaluated by examination of milk and cheese curd samples from cheese manufacturing plants and finished cheddar and italian cheeses. the incidence of y. enterocolitica was lower in cheese curd samples (9.2%) than in raw milk (18.2%). most of the curd samples showed a positive phosphatase test, indicating production from raw mi ... | 1978 | 211937 |
| isolation, characterization, and synthesis of a corticotropin-inhibiting peptide from human pituitary glands. | a corticotropin-inhibiting peptide (cip) has been isolated from human pituitary extracts. it consists of 32 amino acids with a proposed sequence identical to residues 7--38 of corticotropin (acth). the peptide has been synthesized by the solid-phase method. the melanotropic activity of the peptide is estimated to be 30% of the potency of acth. it is devoid of corticosteroidogenic activity but is able to inhibit acth-stimulated corticosterone production in isolated rat adrenal cells. | 1978 | 212744 |
| relative insensitivity to glucagon of sterol synthesis in cultured rat aortic smooth muscle cells. effect of dibutyryl cyclic amp. | the smooth muscle cell plays an important role in the process of atherogenesis. in these experiments the effect of glucagon and dibutyryl cyclic amp on sterol synthesis in cultured rat arterial smooth muscle cells was studied. glucagon in concentrations of 1 x 10(-9) mol/l inhibited the incorporation of sodium (2(-14)c)acetate into non-saponifiable lipids and digitonin precipitable sterols but lower concentrations of glucagon had no effect. in cells which were exposed to serum, dibutyryl cyclic ... | 1978 | 213333 |
| conversion of rubratoxin b to the carboxylic acid derivative and its effect on adenosine triphosphatase activity and toxicity to mice. | | 1978 | 213356 |
| a comparison of some enzyme activities associated with plasma membrane preparations from the cortex and outer medulla of the rat kidney. | | 1979 | 217748 |
| [blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus]. | in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ... | 1979 | 219399 |
| respiratory viruses in hospital patients on the witwatersrand. a 7-year study. | virological investigations were carried out on 4 151 patients with respiratory disease hospitalized between may 1966 and april 1972. the groups examined were black and white children and black miners. influenza viruses were more common among malnourished black children and tended to cause more severe disease. this was also true of adenovirus and herpesvirus hominis type 1 infections. adenoviruses appear to be secondary invaders, frequently after a measles or influenza attack. a generalized epide ... | 1979 | 219551 |
| a reminiscence of dr sarah elizabeth stewart, 1905-1976. | | 1978 | 228140 |
| intracellular ion activities and their relationship to membrane properties of tight epithelia. | | 1979 | 228986 |
| new classes of viable deletion mutants in the early region of polyoma virus. | viable mutants of polyoma virus have been isolated which have deletions in defined parts of the early region of the genome. one class of mutants has deletions (less than 1% of viral genome length) located between 71.5 and 73.5 on the physical map of polyoma virus dna, near the origin of replication. these mutants appear to grow and to transform cells in a manner indistinguishable from wild-type virus. a second type of mutant with deletions (about 2% of viral genome length) located between about ... | 1979 | 229246 |
| [ultrastructural characteristics of soft tissue rhabdomyosarcomas]. | seven rhabdomyosarcomas of human soft tissues were studied electron microscopically. certain ultrastructural features characteristic of tumour rhabdomyoblasts were revealed: atypical fibrillar apparatus, spatially uneven process of myofibrillogenesis, disturbed cyto- and histotypical differentiation ratio and others. these features may be used for electron microscopic diagnosis of rhabdomyosarcomas among soft tissue tumours of low differentiation. | 1979 | 229810 |
| mitochondrial and cytosolic localization of a single glycerate kinase in rat kidney cortex. | the distribution of glycerate kinase [atp:d-glycerate 2-phosphotransferase, ec 2.7.1.31] in kidney was studied. this enzyme was found to be present in the renal cortex. by differential centrifugation of the homogenate and sucrose density gradient analysis, it was found that 42% and 60% of the renal glycerate kinase were localized in the cytosol and mitochondria, respectively. the mitochondrial enzyme appeared to be present in the inner membrane and/or matrix. no difference was found between the ... | 1979 | 230183 |
| interaction of infectious bursal disease and coccidiosis in layer replacement chickens. | during a floor-pen study on anticoccidial programs for layer-replacement pullets, there was a natural outbreak of infectious bursal disease (ibd). the interaction of coccidiosis and ibd was characterized by: excessive deaths from coccidiosis; and apparent failure of some anticoccidial drug treatments. immunity to coccidiosis was not blocked by the ibd infection, although immunity was less in some treatments than would be expected with the degree of coccidiosis exposure. | 1979 | 232661 |
| beta-lactamases from yersinia enterocolitica. | two beta-lactamases, a and b, have been shown to be present in a strain of yersinia enterocolitica (w222). beta-lactamase a hydrolyses a variety of penicillins and cephalosporins. this enzyme is sensitive to thiol reagents, is only partially inhibited by 0-1 mm-cloxacillin and has a molecular weight of approximatley 20,000.beta-lactamase b shows strong cephalosporinase activity but does not hydrolyse some of the penicillins. it is more resistant than beta-lactamase a to thiol reagents, is comple ... | 1975 | 237976 |
| [influence of co2 and ph on the attachment response of the cercaria of diplostomum spathaceum (trematoda) (author's transl)]. | 1. the attachment of the cercaria to artificial substrates (offered via dialyzing membranes) in definite media was investigated under conditions of variable ph and [co2]. 2. a decrease of the ph of the substrate releases only attachments in co2 containing media and consequently acts via co2 systems of the medium. 3. as effective components of co2 systems, dissolved co2 + h2co3 are confirmed. 4. the sensitivity of the reaction on gradients of the co2 partial pressure (in solution) could be establ ... | 1975 | 238346 |
| microsomal ethanol oxidation: activity in vitro and in vivo. | studies by several investigators have confirmed that the microsomal fraction of mammalian liver oxidizes ethanol to acetaldehyde in a reaction that requires nadph and oxygen. efforts to identify the enzymes involved have produced conflicting opinions of the reaction mechanism, however. initially, the microsomal mixed function oxidase system was assumed to be capable of oxidizing ethanol in a mechanism that did not involve either alcohol dehydrogenase or catalase. later evidence suggested that th ... | 1975 | 238369 |
| a calcium ion-dependent atp pyrophosphohydrolase in physarum polycephalum. | an activity of atp pyrophosphohydrolase (ec 3.6.1.8) was found in the soluble fraction of the plasmodium of physarum polycephalum. the products of the enzyme reaction were inorganic pyrophosphate and 5'-amp in equimolar quantities. the enzyme had a pronounced requirement for ca2+ with high specificity. mg-2+ was not an essential cofactor but stimulated the enzyme activity about 2.5-fold of the control. the enzyme hydrolyzed itp, gtp and beta,gamma-methylene atp at a limited rate. among inhibitor ... | 1975 | 238634 |
| the selectivity of beta-adrenoceptor antagonists on cardiovascular and bronchodilator responses to isoprenaline in the anaesthetized dog. | 1 the actions of five beta-adrenoceptor antagonists, chosen because of reported differences in their selectivities, were compared using the positive chronotropic, vasodepressor and bronchodilator responses to isoprenaline in anesthetized dogs. 2 propranolol was a potent antagonist of the isoprenaline responses in all three systems. 3 practolol and acebutolol (m & b 17,803) blocked the positive chronotropic responses to isoprenaline to a greater extent than the vasodepressor or bronchodilator res ... | 1975 | 238697 |
| [improvements in the technical conditions for the evaluation of an elastolytic activity. the role of tryspin as an activator (author's transl)]. | an isotopic assay of elastolytic activity is performed; the iodine-125 ions retained inside the elastin framework were removed by appropriate treatment. this acute substrate enables us to study the role of trypsin on elastase activity and facilitates the study of elastase role in atherosclerosis. | 1975 | 238767 |
| phosphoenolpyruvate hydrolase activity of rabbit muscle pyruvate kinase. | rabbit muscle pyruvate kinase catalyzes the hydrolysis of p-enolpyruvate at the same active site which catalyzes the physiologically important kinase reaction. the hydrolase activity is lower than the kinase activity by a factor of at least 10(3). there are specific monovalent cation and divalent cation requirements. no other cofactors are required. the relative activation of the pyruvate kinase for the hydrolase reaction is: ni(ii) greater than co(ii) greater than mg(ii) greater than mn(ii). th ... | 1975 | 238999 |
| human brain in tissue culture. ii. studies of long-term cultures. | this paper describes the techniques used to maintain and reconstitute from storage adult human brain cells in culture. growth characteristics, cell morphology, lifespan, and karyotypic analysis of cell lines derived from patients with multiple sclerosis (ms), non-ms neurologic diseases, and normal brains are compared. | 1975 | 239029 |
| the interaction of calcium and magnesium ions with deoxyribonucleic acid. | | 1975 | 240314 |
| the autoregulatory capability of galen's rete cerebri and its connections. | the rete, a vascular system between the extra- and intracerebral arteries of certain animals, may have a variety of functions in different species. the calibres of the vessels of the rete and its arteries of supply have been measured under different conditions of blood pressure and paco2. it has been shown that the rete and its afferent arteries behave in an autoregulatory fashion down to a blood pressure of 60 mmhg. they also dilate with a rise in paco2. it has also been found that some parts o ... | 1975 | 241946 |
| the effect of temperature and mechanical work load on the intracellular ph and buffer capacity of cardiac muscle in rats. | the effect of temperature on hydrogen ions and buffer capacity in extracellular fluid differs from the effects in intracellular fluid. in our experiments cardiac hypertrophy was achieved by increasing the mechanical work load. it could be demonstrated that there is a certain correlation between buffering of hydrogen ions and mechanical activity of heart muscle. | 1975 | 242045 |
| [semiautomatic method of measurement of serum 5'nucleotidase activity]. | a semi-automated method for determining the 5'-nucleotidase activity in human serum for use with the technicon autoanalyzer i is described. based upon the manual method of persijn and van der slik, adenosine formed by hydrolysis of 5'-amp is deamined enzymatically and ammonia determined with the berthelot reaction. the non specific alkaline phosphatases are inhibited by phenylphosphate. concentrations of 5'-amp and phenylphosphate are discussed. nucleotidase activity is determined without diluti ... | 1975 | 242242 |
| infant nutrition and obesity. | | 1979 | 257521 |
| patellar fracture in a domestic rabbit (a case report). | | 1979 | 260577 |
| effects of beclomethasone dipropionate on tracheal mucous velocity. | beclomethasone dipropionate (200 micrograms, administered by inhalation to conscious sheep, does not affect tracheal mucous velocity during a 60-min period. | 1977 | 262102 |
| changes in rat lung produced by olivine dust inhalation. | | 1979 | 262291 |
| studies on the tissue specificity of a circulating renotropic factor. | in 1970, it was found that sera removed from rats 24 hr after unilateral nephrectomy stimulate the incorporation of 3h-thymidine into the dna of incubating rat renal cortex. the same sera did not influence isotope incorporation into the dna of incubating liver, spleen, and lung tissue. later, a substance was described in renal tissue that enhanced the action of the renotropic factor in sera. it was proposed that this tissue factor activated the circulating renotropic substance and that lack of t ... | 1979 | 263165 |
| structural studies of synthetic filaments prepared from column-purified myosin. | synthetic filaments prepared from column-purified rabbit skeletal myosin by slow dialysis exhibit characteristic bipolar organization and 14-nm axial subunit spacing. backbone substructure can be discerned in high resolution micrographs in the form of striations of 3--4-nm width and slight angular tilt from the direction of the filament axis. filament backbone diameters vary over the population, although remaining relatively constant for a single filament. approximately 25% of the filaments appe ... | 1979 | 263630 |
| lymphocyte mitogenesis induced by a mammalian liver protein that specifically binds desialylated glycoproteins. | a purified rabbit liver membrane protein that binds desialylated glycoproteins has been shown to be a mitogen for human peripheral lymphocytes. the mitogenic activity is specific for desialylated thymus-derived (t)-cells. the loss of mitogenicity upon exposure of the binding protein to neuraminidase and the inhibitory potency of asialo-orosomucoid support the conclusion that the site involved in the binding of asialoglycoproteins is also responsible for the mitogenic effect on desialylated lymph ... | 1977 | 265530 |
| characteristics of blast crisis in chronic granulocytic leukemia. | the terminal phase of most patients with ph1-positive chronic granulocytic leukemia (i.e., blast crisis) resembles acute leukemia. the clinical and hematologic features of blast crisis in 73 patients with chronic granulocytic leukemia have been reviewed. two major morphological subgroups, lymphoblastic and myeloblastic, were identified. the lymphoblastic group in general had more profound thrombocytopenia and a greater number of blasts, while the myeloblastic group had more severa anemia. extram ... | 1977 | 265737 |