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[pathogenicity factors of gramnegative enteropathogenic bacteria and pathogenesis of intestinal diseases (author's transl)].enterotoxins as produced by the majority of enteropathogenic bacteria play a central role in the pathogenesis of cholera and e. coli enteritis but are obviously of little significance as to the development of salmonella enteritis and dysentery. pathology of salmonella enteritis and dysentery results from multiplication of bacteria within the bowel wall and from the damage of tissue caused thereby. the lipopolysaccharides (endotoxins) of invasive enteropathogenic bacteria do not possess the chara ...19751106437
pathogenesis of shigella diarrhea. serum anticytotoxin antibody response produced by toxigenic and nontoxigenic shigella dysenteriae 1.the serum antitoxin response to the cytotoxin contained in preparations of shigella dysenteriae 1 (shiga's bacillus) exotoxin was studied in natural and experimental infections of man. natural infection resulted in the rapid appearance of toxin-neutralizing antibody, which disappeared some time between 9 and 18 mo after infection. experimental infection of human volunteers provided the opportunity to study immunoglobulin class of the antibody in sera obtained serially from 7 to 50 days after inf ...19761107354
[an analysis of meat products as possible factors responsible for transmission of dysenterial toxic infections].the paper carries experimental data derived from a bacteriological analysis of infected minced meat products for the presence of shigella sonnei and for ascertaining technological stages non-compliance with whose proper course presents the greatest epidemiological hazards. the procedures employed in analyzing infected meat products are shown to be sufficiently sensitive, for they enable one to count shigella dysenteriae in numbers of 102-106/g, depending upon the degree of total bacterial contam ...19751108439
letter: reactions of cartilage. 19751113834
the measurement of iron-binding capacity in serum and purified transferrin with the aid of chemical affinity chromatography.in a mew method for the estimation of transferrin by iron-binding capacity iron is added as the tartrate in nacl with about 10 mm bicarbonate. the excess iron is removed by passage through deae-sephadex a-50 previously treated with the iron chelator disodium catechol-3,5-disulphonate. the iron remaining bound to transferrin is measured without protein precipitation by the use of ferrozine. the method is applicable to fresh, frozen, or lyophilized serum, purified transferrin, and some quality con ...20051127111
fiberoptic bronchoscopy following thermal injury.in a series of 25 burned patients, 15 had inhalation injury as evidenced by fiberbronchoscopic examination. the criteria for post-thermal pulmonary injury were airway edema and inflammation, mucosal necrosis and presence of soot and charring in the airways. this technique of direct visualization of the pulmonary tree can be performed easily, without complications, and provides an objective means for the early diagnosis of inhalation injury in burned patients and prompt institution of treatment.19741129663
[bloch-sulzberger syndrome]. 19761130070
isolation of cyclic 3',5'-pyrimidine mononucleotides from bacterial culture fluids. 19751147989
[sputum cytology and pulmonary reactions to air pollution]. 20051169496
[experiencie with our disaster service]. 20051176317
[morphology of the hemolytic-uremic syndrome]. 20051196095
dermo--epidermal adhesion and its effect on epidermal structure in the mouse.evidence is presented that dermo--epidermal adhesion is low in mouse ear. consequently the pressure caused by mitotic activity in the basal epidermal layer is readily relieved by cell extrusion distally and the number of basal cells per unit skin area remains low. the rule then is that the number of distal cells per unit skin area must be equally low, and the epidermis is therefore thin. conversely, dermo--epidermal adhesion is high in mouse sole-of-foot epidermis, and the mitotic pressure leads ...19751201191
kinetic studies of 51cr and df32p labelled granulocytes.in vivo kinetic studies of granulocytes labelled in vitro with 51cr and df32p were carried out in nine haematologically normal subjects by isolation of the cells in the blood samples by the ficoll-isopaque flotation method. 51cr and 32p specific activity of blood samples made of 93-98% granulocytes was studied. distribution between marginated and circulating granulocyte pools was identical for both labelled cells and the marginated pool was similar to the circulating pool, except that it was low ...20051201203
[changes in the composition of intracellular lipids and hydrocarbons in candida tropicalis in the absence of growth].the fatty acid composition of lipids and the composition of hydrocarbons were studied in candida tropicalis cultivated on a medium with propionic acid and incubated in the conditions of starvation and on a medium containing glucose-1-6-14c but no nitrogen. intracellular fatty acids with an odd number of carbon atoms were found to be easily metabolized by yeast cells. the content and composition of intracellular hydrocarbons were very labile and underwent intensive transformation caused by change ...19751214607
[lower lip and cleft lip with "pits" (van der woude syndrome). study in a family (author's transl)]. 19551215696
[epidemic hemorrhagic conjunctivitis]. 20051230940
platelet monoamine oxidase activity in patients with chronic schizophrenic illnesses untreated by neuroleptic drugs [proceedings]. 20051234010
epithelio-mesenchymal interface during mouse kidney tubule induction in vivo.transmission and scanning electron microscopy were used to study the epithelio-mesenchymal interface between the interacting mouse ureter-bud and the metanephric mesenchyme. the gap between the epithelial and mesenchymal cells varied in width. at the stalk of the ureter-bud the interspace was often about 1 mum, but in the inductively active areas at the tips of the branching ureter-bud epithelio-mesenchymal contacts were seen through discontinuities in the basal lamina. at these points the gap b ...19751240119
circadian properties of the rhythmic system in individual nucleated and enucleated cells of acetabularia mediterranea. 20051248525
[diagnostic problems in gout in a 44-year-old man]. 19761251615
[psychiatric side-effects of levodopa treatment]. 20051265908
production of heteroagglutinins in haemocytes of leucophaea maderae l.it was our purpose to study the immunological activity of circulating fluid cells in the blattoid insect leucophaea maderae l. these cells are generically called haemocytes; after a preliminary morphological study under optical microscope, they were treated with anti-heteroagglutinins serum marked with fluorescein isothiocyanate. thus, it was possible to show the presence of heteroagglutinins into the cytoplasm, as regards one group of haemocytes, and on the cell membrane for the second group.19541269671
radioimmunoassays for free and conjugated trienbolone and for trienbolone acetate in bovine tissue and plasma samples.a specific, sensitive, precise and accurate radioimmunoassay has been developed for the quantitation of the synthetic anabolic steroid trienbolone acetate (tba) and its major metabolites, free and conjugated trienbolone (tboh) in bovine tissues and plasma. with the extraction procedure described unspecific interference with the antigen-antibody reaction could be ruled out. the assay can significantly detect amounts of more than 40 pg tboh and 70 pg tba. 0.1 - 2.0 g tissue and 0.1 - 1 ml plasma a ...19761273899
neonatal tetanus: a developmental follow-up study. 20051278947
exchange transfusion in a case of severe theophylline poisoning. 20051287972
[research on sterilization of pathogens by high electrostatic voltage method].an experimental research has been carried out on the sterilization of four kinds of pathogens by high electrostatic method along with an inquiry into the influence of voltage waveform and the treated time on sterilization. it is concluded that pathogens can be killed efficiently by corona discharge field.19921294177
bacterial endotoxins as potential antitumor agents. tumor mass loss in mice treated with bacterial lipopolysaccharides of shigella dysenteriae serovar 1.natural and modified preparations of lipopolysaccharides and lipopolysaccharide-protein complexes isolated from the s- and r-form of shigella dysenteriae serovar 1 were found to markedly inhibit the initial growth of mouse solid tumors derived from németh-kellner lymphoma, gardner 6c3hed lymphoma, an ill-defined syngeneic lymphoma of dba mice (skalsky lymphoma) and lp-2 plasmacytoma. the biopreparations were given intraperitoneally, most frequently at a dose range from 50 to 200 micrograms per m ...19921296928
composition of lipid a in the s and r forms of shigella dysenteriae serovar 1.a chemical analysis was performed of lipid a, isolated by acid hydrolysis of the lipopolysaccharide from the s and r forms of shigella dysenteriae serovar 1. differences in the moiety of both lipids and sugars were compared. the lipid portion consisted of a homologous series of fatty acids ranging from c12:0 to c18:0 (predominant homologues, c12:0, c14:0 and c16:0) and the homologous series of 3-hydroxy acids ranging from c12:0 to c16:0 (predominant homologue, 3-oh-c14:0). the major sugar portio ...19921303686
[recombinant human erythropoietin]. 19921307066
a 4-kilobase congo red binding plasmid dna fragment of shigella dysenteriae 1 suppresses the growth and cell differentiation in escherichia coli.a 4-kilobase congo red binding plasmid dna fragment of pcat 120 of shigella dysenteriae 1 was transferred to an escherichia coli k12 strain by transformation. transformants were unable to grow in any liquid broth medium. electron microscopic studies revealed that the transformants grown on tryptic soy agar were associated in clusters after cell division. normal cell separation among the transformants in comparison with recipient e. coli k12 was only observed when the growth medium was supplement ...19921307443
in situ hybridization of calcium/calmodulin dependent protein kinase ii and tau mrnas; species differences and relative preservation in alzheimer's disease.abnormal phosphorylation of the microtubule associated protein tau component of neurofibrillary tangles (nfts) in alzheimer's disease (ad) may result from alterations in protein kinase expression. calcium/calmodulin dependent protein kinase ii (cam kinase ii) has been shown to phosphorylate tau in vitro in such a way to decrease its electrophoretic mobility. a68, apparently a modified form of tau in ad brain, also shows abnormal phosphorylation and slower mobility than tau. to further examine th ...19921312209
[clinical studies for usefulness of gd-dtpa enhanced mri in lung cancer].to evaluate utility of gd-dtpa enhanced mri (gd-mri) in lung cancer, gd-mri was performed in 69 cases. 1) viable tumor was strongly enhanced, necrosis in the tumor, however, was not enhanced on gd-mri. enhanced patterns of gd-mri were divided into 3 types, however there was little correlation between the enhancement patterns and histologic types. 2) in serial scan studies of 15 cases, the signal intensity of the tumor reached the peak 3 minutes to 10 minutes after gd-dtpa administration, and aft ...20051315952
which twin has the toni? commentary on j. d. sinclair. 19921317159
the membrane receptor for sex steroid binding protein is not ubiquitous.the tissue distribution of the membrane receptor for the sex steroid binding protein (sbp) has been studied, either in estrogen/androgen dependent tissues and in tissues not strictly sex steroid dependent. a specific interaction of sbp with cell membranes has been observed to occur only in estrogen/androgen dependent tissues, some of them had been previously shown by our laboratory and by other authors to possess a specific receptor for the protein. thus, the sex steroid dependence of the tissue ...19731331225
[bacteriology of shigella isolated in a tropical zone chu].from 1981 to 1991, five hundred eighty one shigella's strains were isolated in the laboratories of the chu fann of dakar. they represent 24.8 per cent of enteropathogen agents and 4.3 per cent of diarrhoeae causative agents. shigella flexneri was predominant (71%), following by shigella dysenteriae (14.5%), shigella sonnei (11.4%) and shigella boydii (2.5%). in pediatric hospital 90 per cent of strains were isolated between 1 and 5 years age; in adults, the pic was found from 20 to 30 years. fiv ...19921345084
sexual transmission of htlv infections in southern india. 19921346809
molecular probe analysis of shigella dysenteriae type 1 isolates from 1940 to 1987.fourteen strains of shigella dysenteriae type 1 (shiga bacillus) isolated from people in diverse locations from 1940 to 1987 were studied. southern hybridization with three cloned escherichia coli genes, shiga-like toxin i (slti), frd, and ompf, was used to determine restriction fragment length polymorphism (rflp) of the genomic dna of these strains. digestion with each of four restriction endonucleases generated fragments of identical size to which the frd and ompf hybridized for each of the 14 ...19921353066
molecular epidemiology of shigella infections in israel.the dnas of shigella sonnei or shigella dysenteriae type 1 strains isolated in outbreaks of shigellosis or in sporadic cases were analysed by restriction fragment length polymorphism (rflp). southern blots of the dnas of 36 s. sonnei isolates digested by 8 restriction enzymes were hybridized with an escherichia coli rrna probe. the s. sonnei strains were unexpectedly diverse in their rflp. antibiotypes of the same isolates showed clusters of strains corresponding to the various outbreaks. on the ...19921356821
pregnancy after transfer of frozen-thawed human embryos.cryopreservation of human embryos has been successfully applied in in vitro fertilization (ivf) and embryo transfer (et) programs at the national taiwan university hospital since 1988. our preliminary results with 120 frozen-thawed embryos in 31 transfer cycles showed that the survival rate of frozen embryos was 66%. following transfer, the implantation rate and clinical pregnancy rate were 6.5% and 13%, respectively. four clinical pregnancies and one preclinical pregnancy following a frozen-tha ...19921360300
[lipid and mineral metabolism and morbidity with temporary loss of work capacity in miners].examination is performed on 87 miners from mine for copper production subject to intensive local vibrations with average rate of increase (k) of the norms for the individual octave frequencies 2.4 times for 3 h exposure of the working shift, as well as to intensive noise--113-115 db/a equivalent level, general dust--23 mg/m3, fine dust--3.5 mg/m3 and fine quartz--0.43 mg/m3. there are data for vibration effect--changes in the cold test, vibration sensoriness, ultra sound sonometry, in all miners ...20051364539
a comparison of therapeutic schedules for administering granulocyte colony-stimulating factor to nonhuman primates after high-dose chemotherapy.granulocyte colony-stimulating factor (g-csf) has been shown to be effective in clinical trials for reducing the period of neutropenia after chemotherapy. in this study, we compared the timing for initiating g-csf administration after chemotherapy with the duration of neutropenia and hematopoietic regeneration. nonhuman primates treated with high-dose chemotherapy (mechloroethamine, 1.5 mg/kg, intravenously) and not administered g-csf therapy experienced 8 days of neutropenia (absolute neutrophi ...19921373969
outward currents in longitudinal colonic muscle cells contribute to spiking electrical behavior.electrical events in longitudinal and circular muscles of the colon are different. longitudinal muscles generate action potentials superimposed upon small depolarizations termed myenteric potential oscillations and circular muscles generate slow wave events that persist for several seconds. differences between circular and longitudinal muscles may be related to the potassium channels these cells express. we have studied ca(2+)-dependent and voltage-dependent k currents of isolated longitudinal c ...19921378997
immunoblot analysis of antibody responses to shigella dysenteriae type 1 infection.immunoblot analysis was used to identify the antigenic components of the outer membrane protein (omp) extract from sh. dysenteriae type 1 that may be relevant in protection against infection. the omps were extracted by ultrasonic disruption followed by sarkosyl extraction. the macromolecular bands were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), electroeluted to a nitrocellulose matrix and complexed with peroxidase conjugated antibodies in human convalesce ...19921380491
stereoselective syntheses of a di-, tri-, and tetra-saccharide fragment of shigella dysenteriae type 1 o-antigen using 3,4,6-tri-o-acetyl-2-azido-2-deoxy-alpha-d-glucopyranosyl chloride as a glycosyl donor.methyl 2,4-di-o-benzoyl-alpha-l-rhamnopyranoside (1) furnished a crystalline 3-o-bromoacetyl derivative that was treated with the dichloromethyl methyl ether-zncl2 reagent to give 2,4-di-o-benzoyl-3-o-bromoacetyl-alpha-l-rhamnopyranosyl chloride (3). compounds 1 and 3 were condensed under the conditions of base-deficient, silver trifluoromethanesulfonate-mediated glycosylation to give a fully protected rhamnobioside, which on o-debromoacetylation afforded the disaccharide nucleophile 10. similar ...19921381283
carbohydrate recognition molecules on lymphocytes. 19961383059
role of escherichia coli k-12 rfa genes and the rfp gene of shigella dysenteriae 1 in generation of lipopolysaccharide core heterogeneity and attachment of o antigen.the rfp gene of shigella dysenteriae 1 and the rfa genes of escherichia coli k-12 and salmonella typhimurium lt2 have been studied to determine their relationship to lipopolysaccharide (lps) core heterogeneity and their role in the attachment of o antigen to lps. it has been inferred from the nucleotide sequence that the rfp gene encodes a protein of 41,864 da which has a structure similar to that of rfag protein. expression of this gene in e. coli k-12 results in the loss of one of the three ba ...19921385388
a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells.members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ...19921391942
oral anticoagulation therapy: indications, contraindications, and complications.the goal of oral anticoagulation therapy is to prevent thromboembolic events while minimizing the possibility of hemorrhage. this article discusses indications for, contraindications to, and possible complications of anticoagulant therapy. guidelines for the use of low intensity and high intensity anticoagulation and duration of anticoagulant therapy are also included.20061392676
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
identification of a b subunit gene promoter in the shiga toxin operon of shigella dysenteriae 1.the shiga toxin operon (stx) is composed of a and b subunit genes which are transcribed as a bicistronic mrna from a promoter which lies 5' to the stxa gene. northern (rna) blot and primer extension analyses revealed the existence of a second stxb gene transcript. recombinant plasmids which carried the stxb gene without the stx operon promoter and with the influence of a vector promoter abrogated produced stx b polypeptides, suggesting that the stxb gene mrna was transcribed from an independent ...19921400202
the cyclosporin related toxicity in high risk liver transplant patients. 19681409299
upper gastrointestinal bleeding from merkel cell carcinoma.merkel cell carcinoma is a rare small cell carcinoma of the dermis with aggressive clinical features and frequent local recurrence. intra-abdominal spread of the cancer has previously been noted primarily in the liver and retroperitoneal nodes. we report a patient with previously diagnosed merkel cell carcinoma of the right buttock with metastases to the stomach and duodenum presenting with acute upper gastrointestinal bleeding.19951415105
treatment of shigellosis: iii. comparison of one- or two-dose ciprofloxacin with standard 5-day therapy. a randomized, blinded trial.to determine whether a single dose, or 2 doses, of ciprofloxacin are as effective as 5-day, 10-dose therapy for the treatment of shigellosis in adult men who are moderately to severely ill.19921416574
decreased food intake in children with severe dysentery due to shigella dysenteriae 1 infection.factors that affect food intake in acute shigellosis were studied in 82 children aged 24-59 months. children were offered an energy-dense milk-cereal-oil-based diet every 2 h. food intake was compared between children with shigella dysenteriae 1 infection and those infected with other shigella spp (predominantly s. flexneri). mean energy intake in the first 48 h was 435 kj/kg.d in children infected with s. dysenteriae 1 and 536 kj/kg.d in children infected with other shigella spp (p < 0.001). fe ...19921425537
regulation of the slt-1a toxin operon by a ferric uptake regulatory protein in toxinogenic strains of shigella dysenteriae type 1.the regulation of shiga toxin expression in a clinical isolate of s. dysenteriae 1 by the fe-fur (iron-ferric uptake regulatory protein) repressor complex was investigated. the presence of an endogenous fur repressor protein capable of binding to either a fur binding consensus sequence or the regulatory region of slt-1a was determined in toxinogenic strains of s. dysenteriae. plasmid constructs bearing fur binding sites fused to readily assayable reporter genes were used. plasmid psc27.1 contain ...19921430967
[shigella dysenteriae strains having a provisional serovar isolated from imported diarrheal cases in tokyo].two bacterial strains (me448 and me474) isolated from stool cultures of imported cases in tokyo in 1987 had typical biochemical characteristics of shigella dysenteriae. the results of antigenic analyses showed that they were serologically identical to each other, but did not belong to any of the established shigella serovars. these strains were positive for serény test in guinea pig eye and cell-invasion test in hela cells. the strains also had virulence-plasmid encoding outer membrane proteins, ...19921431369
mitogenic effects of human recombinant insulin on b-cell precursor acute lymphoblastic leukemia cells.insulin and the insulin-like growth factors (igf-i, igf-ii) constitute a family of peptides capable of stimulating diverse cellular responses, including cell proliferation. in order to determine the effects of these peptides on malignant cells, we analyzed the expression and function of insulin, igf-i, and igf-ii receptors on b-cell precursor acute lymphoblastic leukemia (bcp all) cell lines, utilizing competitive binding, affinity crosslinking, and cell proliferation assays. the bcp all cells b ...19921434795
incidence and level of exotoxin a in proteolytic cell-free cultures of clinical isolates of pseudomonas aeruginosa collected from hospitals in riyadh, saudi arabia.mice lethality bioassay revealed that out of 102 proteolytic cell-free cultures of pseudomonas aeruginosa only 17 (15%) were lethal with values of 19-48 ld50/ml. antitoxin a antiserum neutralized the lethality providing evidence that the main lethal compound in the cell free cultures was exotoxin a. the neutralization also ruled out the lethal contribution of protease, elastase and phospholipase c. the presence of exotoxin a in a proteolytic environment may indicate less efficient proteolytic ac ...19921435349
effect of ethylenediamine di-o-hydroxyphenylacetic acid and transferrin on the growth of some bacterial strains in vitro.the ability of some bacterial strains to obtain iron from ethylenediamine di-o-hydroxyphenylacetic acid (edda) or iron-free transferrin, and accordingly grow in their presence, was studied. growth of staphylococcus aureus and yersinia pseudotuberculosis was inhibited by edda or by iron-free transferrin. growth of streptococcus faecalis, however, was inhibited by iron-free transferrin, but not by edda. the other bacterial strains, i.e.; escherichia coli, salmonella typhimurium and shigella dysent ...19921435350
mixed connective tissue disease presenting as a left sided pleural effusion.mixed connective tissue disease (mctd) usually occurs in women aged 13-50 years. pleural effusion is rarely the presenting feature of this disease. the case is reported here of a male patient with mctd who presented at the age of 79 years with a left sided pleural effusion.19841444629
cloning and sequencing of a gene encoding a 21-kilodalton outer membrane protein from bordetella avium and expression of the gene in salmonella typhimurium.three gene libraries of bordetella avium 197 dna were prepared in escherichia coli le392 by using the cosmid vectors pcp13 and pya2329, a derivative of pcp13 specifying spectinomycin resistance. the cosmid libraries were screened with convalescent-phase anti-b. avium turkey sera and polyclonal rabbit antisera against b. avium 197 outer membrane proteins. one e. coli recombinant clone produced a 56-kda protein which reacted with convalescent-phase serum from a turkey infected with b. avium 197. i ...19921447140
shigellosis in children: a prospective hospital based study.from 1985 to 1988, fecal samples of 950 hospitalized children suffering from diarrhea or dysentery were screened for shigella species using standard methods. shigella species were isolated as sole pathogen from 192 (20.2%) cases and s. flexneri type 2 was the predominant serotype. shigella infection was prevalent throughout the year with high isolation rate during the summer and early monsoon months. shigella strains isolated during the period were resistant to most of the commonly used drugs fo ...19921452309
detection of shigella dysenteriae type 1 and shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction.a combination of immunomagnetic separation (ims) and a polymerase chain reaction (pcr) procedure was used for direct isolation and identification of shigella dysenteriae type 1 and shigella flexneri from feces. immunomagnetic particles were coated with monoclonal antibody masfb, which is specific for a common epitope of the o polysaccharides of s. dysenteriae type 1 and s. flexneri. bacteria bound to the beads were boiled in water, and target dna was amplified with a primer pair specific for a g ...19921452650
[incidence, frequency and resistance characteristics of methicillin-oxacillin resistant staphylococcus aureus strains in germany].in a multicentre study, the methicillin-resistant staphylococcus aureus (mrsa) isolates in 19 large clinics in germany were recorded, and the resistance characteristics of these strains were studied. oxacillin-mannitol-salt agar plates were distributed to all participants to ensure uniformity of screening, and each laboratory used these plates to investigate 200 consecutive staphylococcus aureus isolates for oxacillin-methicillin resistance. of the 3,794 evaluable staphylococcus aureus isolates, ...19921459021
[bronchobiliary fistula in carcinoma of the hepatic duct bifurcation].carcinoma of the hepatic duct bifurcation was diagnosed in a 67-year-old women with obstructive jaundice. as metastatic spread could not be demonstrated the carcinoma was removed with a view of achieving a cure (hemihepatectomy, resection of the hepatic duct and the bifurcation, cholecystectomy and hepatojejunostomy). histological examination indicated adenocarcinoma of the biliary tract. seven months postoperatively the patient was found to be cachectic and cough up greenish liquid sputum. bili ...20061459022
purification and partial characterization of two azoreductases from shigella dysenteriae type 1.two azoreductases (i and ii) were purified to homogeneity from extracts of shigella dysenteriae (type 1). azoreductase i was a dimer of identical subunits of m(r) 28,000, whereas azoreductase ii was a monomer of 11,000 m(r). both were flavoproteins, each containing 1 mol of fmn per mol enzyme. both nadh and nadph functioned as electron donors for the azoreductases. azoreductase i used ponceau sx, tartrazine, amaranth and orange ii as substrates. azoreductase ii utilized all the dyes except amara ...19921459414
serum antibodies to verotoxin-producing escherichia coli (vtec) strains in patients with haemolytic uraemic syndrome.serological evidence of infection with verotoxin-producing escherichia coli (vtec) was sought in 28 patients suffering from haemolytic uraemic syndrome (hus) and 25 age- and sex-matched controls. elisa was used to detect anti-lipopolysaccharide (lps) antibodies to e. coli strains o157, o111, o26 and nctc 10418, a non-vtec strain, and shigella dysenteriae o1. sera from 19 of the hus patients but from none of the 25 controls had significant antibody levels to the verotoxin-producing bacteria. sera ...19921460654
the effect of insulin treatment on hba1c, body weight and lipids in type 2 diabetic patients with secondary-failure to sulfonylureas. a five year follow-up study.no conclusive data are available about the long-term effect of insulin treatment in type 2 diabetic patients failing to maximal doses of sulfonylureas and caloric restriction. in total 160 non-obese type 2 diabetic patients with secondary failure were substituted with insulin using a diabetic teaching and care program. from these 160 patients 40 died within the observation period of five years; 102 patients had a complete five year follow-up, whereas the remaining 18 patients did not come to reg ...20061464414
[the theoretical and practical interpretations of the basic manifestations of the epidemic process in shigellosis]. 19921466177
[new hybrid proteins--potential immunosuppressive agents]. 19921468359
serological diagnosis of infection by shigella dysenteriae-1 in patients with bacillary dysentery.a total of 192 samples of serum from 113 sri lankan patients with clinical dysentery was examined for antibodies of the igm class to the lipopolysaccharides (lpss) of shigella dysenteriae-1 and escherichia coli o157:h7. by means of elisa and immunoblotting, 59 patients were found to have serum antibodies to the lps of s. dysenteriae-1 only. four samples from one patient were found to contain serum antibodies to the lpss of both s. dysenteriae-1 and e. coli o157:h7. antibodies to the lps of s. dy ...19921474263
[bacillary dysentery--1990]. 19921475396
factors affecting the strength of flexor tendon repair.the effects of different thicknesses and configurations of core sutures were studied in human cadaveric flexor tendon repairs. both straight and cyclic load tests were employed. to exploit the full strength of 4/0 suture material, the kessler repair using four locked single knots would seem to be appropriate.19921479249
experimental and clinical gnotobiotics: influence of the microflora on graft-versus-host disease after allogeneic bone marrow transplantation.one of the major complications of allogeneic bone marrow transplantation (bmt) is graft-versus-host disease (gvhd), which is caused by donor type lymphocytes which react against the recipient's tissues. an important factor which influences gvhd is the recipient's gastrointestinal microflora. this was originally observed in gnotobiotic mice. infusion of 10(7) h-2 incompatible bone marrow cells into lethally irradiated (9.0 gy x-rays) conventional mice results in a late onset type gvhd which cause ...19921479298
[the causes of cerebral complications in patients with ischemic heart disease following myocardial revascularization operations].in the period from jan. 1988 to jan. 1991 operations for the formation of an aortocoronary shunt were carried out in 437 patients at the department of combined affections of the coronary and peripheral arteries, bakulev research institute. in some cases the operation was combined with resection of a postinfarction aneurysm of the left ventricle. cerebral complications developed in the postoperative period in 28 (6.4%) patients. their analysis showed the prevalence of severe forms of consciousnes ...20061482586
incomplete vascular casting for a scanning electron microscope study of the microcirculatory patterns in the rat pancreas.scanning electron microscopy of resin casts prepared by incomplete arterial injections showed that in the rat pancreas, the casting medium fills blood capillaries in the endocrine islets more promptly than those in the exocrine lobules and secretory ducts. furthermore, the exocrine lobules containing endocrine islets allowed a more rapid resin flow through the insulo-acinar portal route than those lobules lacking an islet. the secretory ducts were the last portions to be filled with resin. since ...19921482604
does adequate control of diabetes in the preinfarction period improve the short-term postinfarction course?it is uncertain whether adequate preinfarction diabetes control would alter the clinical outcome in diabetic patients once myocardial infarction has occurred. this study attempts an evaluation. diabetic patients admitted successively to the cardiac intensive care unit with their first acute myocardial infarction were enrolled and followed throughout hospitalization. every fourth consecutive patient with infarction, but not diabetic, was assigned to a control group. all patients were kept in the ...20051482779
chartline: providing bibliographic references relevant to patient charts using the umls metathesaurus knowledge sources.a successful medical informatics program helps its users to match their information needs as closely and efficiently as possible to the capabilities of the system. chartline is a computer program whose input is a free text, "natural language" patient chart in ascii format. using the umls metathesaurus knowledge sources, chartline can suggest bibliographic references relevant to the patient case described in the chart. the program does not attempt to "understand" the natural language content of t ...20061483014
asbestos: the turbulent interface between science and policy. 20061489439
[territorial differences in the nature of the etiological structure of shigellosis--a pattern or chance?]. 19921496882
ventricular fibrillation during acute rejection after heart transplantation.the case of a 46-year-old patient who underwent orthotopic heart transplantation for treatment of end-stage heart failure as a result of ischemic heart disease is reported. four months after transplantation a grade ii rejection episode was complicated by ventricular fibrillation. lidocaine and procainamide intravenously did not effectively prevent recurrence. an increase of antirejection therapy in combination with flecainide acetate effectively prevented further episodes of ventricular fibrilla ...19821498146
gut mucosal lymphocyte subpopulations in the host-defence of shigella infected guinea-pigs.lymphocyte subpopulations from the gut mucosa were studied in shigella-susceptible guinea-pigs to evaluate their role in mucosal immune responses to such organisms. four weeks after infection, isolated lymphocytes were characterised by an immunofluorescent technique. the increase in the t cell intra-epithelial population was less (48%) in comparison to that in normal uninfected animals (74%). on the other hand, ig bearing b cells in the lamina propria showed an increased incidence and more than ...19921500085
integrating general medicine and rheumatology training in the outpatient setting: a practice model.the authors describe a primary care-based educational and practice model that integrates general medicine resident education in outpatient rheumatology with specialty fellowship training. compared with the use of traditional specialty clinics, the model provides better access and service to patients and more appropriate training for residents. revenues from clinical service delivered by faculty-supervised residents and fellows support 80% of the operating costs and educational activities of the ...19811506951
polymerase chain reaction for detection of verocytotoxigenic escherichia coli isolated from animal and food sources.animals and their by-products have been implicated as important sources of verocytotoxigenic escherichia coli (vtec) associated with disease in humans. vtec comprise a wide range of serotypes and produce a variety of closely related verocytotoxins (vt). a pair of oligonucleotide primers, targeting conserved sequences found in vt1, vt2 and vte genes, was used to develop a polymerase chain reaction (pcr) procedure to detect all types of vtec. supernatants of boiled broth cultures of vtec (223 stra ...19921513344
a look at the world. 19721518398
single-dose ciprofloxacin for shigellosis in adults. 19921522320
titres of class-specific antibodies against shigella and salmonella lipopolysaccharide antigens in colostrum and breast milk of costa rican, swedish and vietnamese mothers.enzyme immunoassays (eia) were used to estimate titres of class-specific antibodies against purified and chemically defined phenol-water-extracted lipopolysaccharide (lps) antigens of salmonella serogroup b (bo), shigella dysenteriae type i, plesiomonas shigelloides (the same o-antigen as shigella sonnei) and shigella flexneri y. titres in colostrum and breast milk of swedish, vietnamese and costa rican mothers from various socioeconomic conditions were compared. the antibodies were mainly of th ...19921522329
monitoring and prevention of renal dysfunction in cardiac transplant recipients.renal dysfunction is a common problem in cardiac transplant recipients who receive the immunosuppressive agent cyclosporine. elevation in serum creatinine levels and other indicators of renal dysfunction often appear during the first year after transplant. related problems of hypertension and gout also are common. specific strategies to minimize the nephrotoxic effects of cyclosporine and other potential insults to renal function can be used by nurses, physicians, and other health team members. ...20061524934
solid phase enzyme immunoassays for the quantification of serum amyloid p (sap) and complement component 3 (c3) proteins in acute-phase mouse sera.simple, reliable and sensitive enzyme immunoassays have been developed for the quantification of the mouse acute-phase sap and c3 proteins. the elisa systems were validated using sera from mice injected with s. dysenteriae endotoxin, and detected 500 pg protein/ml. the assays use 96-well microtitre plates permitting rapid processing of a large number of samples.19921526624
monocyte disorders associated with t cell defects in patients with solid tumors.t cells proliferate in response to autologous monocytes in the autologous mixed lymphocyte reaction (amlr). amlr was found to be impaired in patients with advanced cancer (stages iii and iv), whereas normal values were found in the early stages of the disease (stages i and ii). peripheral t lymphocytes from patients with advanced stages also exhibited a decreased ability to produce interleukin-2 (il-2) during an amlr response, whereas production of il-2 by t cells in stages i and ii was comparab ...20061533752
low levels of coagulation inhibitors in patients with clostridium difficile infection.to investigate levels of coagulation inhibitors in sera from patients with clostridium difficile-associated diarrhoea and colitis, commercially available antigen assays were used for immunochemical determination of antithrombin iii, protein c and free protein s. sera from patients with clostridium difficile-associated diarrhoea and colitis showed significantly lowered levels of all measured inhibitors as compared to controls (student's t test). protein c (mean +/- sd): 0.70 +/- 0.30 vs. 1.28 +/- ...19921533851
risk factors for suicide attempts in a human immunodeficiency virus screening program. 19921539099
delirium during intra-aortic balloon pump therapy. incidence and management. 20061539101
heterogeneity analysis of breast cancer families by using age at onset as a covariate.an extension of the usual mixture model of heterogeneity (two family types, one with and one without linkage) is proposed by introducing age at onset as a covariate. the extended model defines age-dependent penetrances where the exact parametrization of age-at-onset distributions depends on the given genotype and family type (linked or unlinked). this extension was applied to breast cancer families. we postulated that the mean age at onset in individuals affected by the linked gene was lower tha ...20061539592
thromboelastogram fails to predict postoperative hemorrhage in cardiac patients.to determine the clinical usefulness of the thromboelastogram in the prediction of postoperative hemorrhage in cardiac patients, we related the results of routine coagulation tests (rcts) and thromboelastography with the amount of chest tube drainage postoperatively in 101 patients requiring cardiopulmonary bypass. our data indicated that there was no correlation between rct results and thromboelastographic variables. no single variable of rcts and thromboelastography correlated well with the am ...20061540061
invasive thymoma with intracaval growth into the right atrium.we report an unusual case of invasive thymoma with intracaval growth into the right atrium. computed tomography and venacavography demonstrated this manner of extension of thymoma. the tumor was completely removed by means of cardiopulmonary bypass after four courses of chemotherapy. multidisciplinary treatment for invasive thymoma with this growth pattern is thought to be useful.20061540072
nucleotide sequence and transcriptional regulation of a positive regulatory gene of shigella dysenteriae.a 1,937 bp psti-hindiii fragment containing the ipar locus was cloned from the large invasion plasmid of shigella dysenteriae cg097, and its nucleotide sequence was completely determined. the ipar protein (35 kda, calculated from the dna sequence) was synthesized in escherichia coli chi 1411 minicells containing the 1,937-bp psti-hindiii fragment. to determine the regulatory role of ipar for ipa genes, we applied genetic complementation experiments using chloramphenicol acetyltransferase (cat) a ...19921541532
shigella-specific iga in saliva of children with bacillary dysentery.to study the secretory immune response after shigella infection, the anti-lipopolysaccharide and anti-shiga-toxin response in saliva, obtained from children with confirmed shigellosis and healthy children, were determined by enzyme-linked immunosorbent assay and by western blot. children with infection showed high titers compared to healthy controls. after shigella dysenteriae type 1 infection a significant change in titer could be observed in a large number of cases, in contrast to shigella fle ...19921547024
drug resistance and plasmid profile of shigellae in taiwan.one hundred and twenty-eight shigella strains isolated from newborn and infant human faecal specimens at kaohsiung medical college hospital in taiwan were serogrouped, serotyped and examined for drug-resistance patterns and for the presence of plasmids. forty-seven per cent of the isolates were found to belong to the shigella sonnei serogroup, 41% to the s. flexneri group, 9% to the s. boydii group and 3% to the s. dysenteriae group. the serotype with the greatest number of strains was s. sonnei ...19921547844
pharmacodynamics of pemoline in attention deficit disorder with hyperactivity.the onset, duration, and offset of pemoline action to improve cognitive performance is examined intensively in 25 prepubescent males suffering from attention-deficit disorder with hyperactivity (addh). the purpose was to characterize the pharmacodynamics of pemoline in addh patients through correlation of plasma pemoline concentration with psychometric measures of memory search efficiency and paired-associates learning, with the physiological effect of pemoline on dopaminergic transmission concu ...20061564025
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