Publications

development of recombinant lactococcus lactis displaying albumin-binding domain variants against shiga toxin 1 b subunit.	infections with shiga toxin-producing bacteria, like enterohemorrhagic escherichia coli and shigella dysenteriae, represent a serious medical problem. no specific and effective treatment is available for patients with these infections, creating a need for the development of new therapies. recombinant lactic acid bacterium lactococcus lactis was engineered to bind shiga toxin by displaying novel designed albumin binding domains (abd) against shiga toxin 1 b subunit (stx1b) on their surface. funct ...	2016	27606705
stable expression of shigella dysenteriae serotype 1 o-antigen genes integrated into the chromosome of live salmonella oral vaccine vector ty21a.	typhoid fever and shigellosis cause high morbidity and mortality worldwide, yet no anti-shigella vaccine is currently available. however, to protect against typhoid fever, an approved vaccine, based on the attenuated salmonella enterica serovar typhi strain ty21a is available. we have investigated ty21a as a live oral vaccine vector for expression of heterologous foreign antigens to protect against other diseases (e.g. shigellosis, anthrax, and plague). shigella lps is a potent vaccine antigen f ...	2016	27655911
erratum: global phylogeography and evolutionary history of shigella dysenteriae type 1.		2016	27694821
active shiga-like toxin produced by some aeromonas spp., isolated in mexico city.	shiga-like toxins (stx) represent a group of bacterial toxins involved in human and animal diseases. stx is produced by enterohemorrhagic escherichia coli, shigella dysenteriae type 1, citrobacter freundii, and aeromonas spp.; stx is an important cause of bloody diarrhea and hemolytic uremic syndrome (hus). the aim of this study was to identify the stx1/stx2 genes in clinical strains and outer membrane vesicles (omvs) of aeromonas spp., 66 strains were isolated from children who live in mexico c ...	2016	27725813
shigellosis in nepal: 13 years review of nationwide surveillance.	shigella is a major cause of gastroenteritis especially in children. in developing countries, the incidence is frequent and results are often life threatening. changing epidemiology and emerging antibiotic resistance warrants continuous monitoring of susceptibility. the present study highlights the changing epidemiology and drug resistance patterns of shigella isolated at different hospitals of nepal over a period of 13 years (jan. 2003-dec. 2015).	2016	27814742
immunization with r-lactococcus lactis expressing outer membrane protein a of shigella dysenteriae type-1: evaluation of oral and intranasal route of administration.	to evaluate the comparative immunogenic potential of food grade lactococcus lactis expressing outer membrane protein a (ompa) of shigella dysenteriae type-1 (sd-1) when administered either orally or intranasally.	2017	27860045
aloe vera (aloe barbadensis miller) supplemented probiotic lassi prevents shigella infiltration from epithelial barrier into systemic blood flow in mice model.	the aim of present work was to investigate preventive role of orally administered aloe vera supplemented probiotic lassi (apl) on shigella dysenteriae infection in mice. at the end of experimental period (2, 5 and 7 days of challenging), different organs such as spleen, liver, small intestine, large intestine, and peritoneal fluid were collected and assessed for shigella colonization. secretary iga was estimated in intestinal fluid. blood was collected in heparinized tubes for various haematolog ...	2017	27914960
a proteomics assay to detect eight cbrn-relevant toxins in food.	a proteomics assay was set up to analyze food substrates for eight toxins of the cbrn (chemical, biological, radiological and nuclear) threat, namely ricin, clostridium perfringens epsilon toxin (etx), staphylococcus aureus enterotoxins (sea, seb and sed), shigatoxins from shigella dysenteriae and entero-hemorragic escherichia coli strains (stx1 and stx2) and campylobacter jejuni cytolethal distending toxin (cdt). the assay developed was based on an antibody-free sample preparation followed by b ...	2017	28008711
facile microwave-assisted synthesis of te-doped hydroxyapatite nanorods and nanosheets and their characterizations for bone cement applications.	in this work, the authors have fabricated the nanorods and nanosheets of pure and te-doped hap with different te concentrations (0.04, 0.08, 0.16, 0.24wt%) by microwave-assisted technique at low temperature. the crystallite size, degree of crystallinity and lattice parameters are calculated. fe-sem study confirms that the fabricated nanostructures are nanorods of diameter about 10nm in undoped and at low concentration of te doping. however, at and higher concentration, it becomes nanosheets of a ...	2017	28024611
characterization of two polyvalent phages infecting enterobacteriaceae.	bacteriophages display remarkable genetic diversity and host specificity. in this study, we explore phages infecting bacterial strains of the enterobacteriaceae family because of their ability to infect related but distinct hosts. we isolated and characterized two novel virulent phages, sh6 and sh7, using a strain of shigella flexneri as host bacterium. morphological and genomic analyses revealed that phage sh6 belongs to the t1virus genus of the siphoviridae family. conversely, phage sh7 was cl ...	2017	28091598
[point-of-care tests for the rapid diagnosis of shigellosis].	worldwide, it is estimated that 140 million people suffer from shigellosis annually. the traditional identification of shigella spp. by culture lacks sensitivity. rapid diagnosis of shigellosis is important because it allows to engage appropriate antimicrobial treatment that shortens the duration and severity of the illness and reduces microbial carriage, thus the spread of infection in the community. onestep immunochromatographic dipstick tests have been successfully developed at institut paste ...	2017	28116567
transcriptional and posttranscriptional regulation of shigella shut in response to host-associated iron availability and temperature.	like most bacteria, shigella must maintain a precise balance between the necessity and toxicity of iron; a balance that is achieved, at least in part, by regulating the production of bacterial iron acquisition systems in response to specific environmental signals. using the shigella heme utilization (shu) system, s. dysenteriae is able to acquire iron from heme, a potentially rich source of nutritional iron within the otherwise iron-limited environment of the human host. investigations presented ...	2017	28127899
sibling srna ryfa1 influences shigella dysenteriae pathogenesis.	small regulatory rnas (srnas) of shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. here, we characterize ryfa1, one member of a sibling pair of srnas produced by s. dysenteriae. unlike its nearly identical sibling molecule, ryfa2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding ryfa1, or a ryfa1-like molecule, is strongly correlated with virulence in a variety of enteropathogens ...	2017	28134784
relationship between drug resistance and the clustered, regularly interspaced, short, palindromic repeat-associated protein genes cas1 and cas2 in shigella from giant panda dung.	to detect drug resistance in shigella obtained from the dung of the giant panda, explore the factors leading to drug resistance in shigella, understand the characteristics of clustered, regularly interspaced, short, palindromic repeats (crispr), and assess the relationship between crispr and drug resistance.	2017	28207509
virulotyping of shigella spp. isolated from pediatric patients in tehran, iran.	shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. in this survey the prevalence of four important virulence genes including ial, ipah, set1a, and set1b were investigated among shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in tehran, iran. the samples were collected during 3 years (2008-2010) from children who were sus ...	2017	28218003
a new phthalide from the endophytic fungus xylaria sp. gdg-102.	a new phthalide derivative, xylarphthalide a (1), along with two known compounds (-)-5-carboxylmellein (2) and (-)-5-methylmellein (3), were isolated from the endophytic fungus xylaria sp. gdg-102 cultured from the chinese medicinal plant sophora tonkinensis. their structures were identified by ms and nmr experiments, and the absolute configuration of 1 was further confirmed by single-crystal x-ray diffraction analysis. compound 1-3 showed antibacterial activities against bacillus megaterium, ba ...	2017	28385040
shigella dysenteriae infection activates proinflammatory response through β-catenin/nf-κb signaling pathway.	shigella dysenteriae (s.dysenteriae) the causative agent of bacillary dysentery invades the human colonic epithelium resulting in severe intestinal inflammatory response and epithelial destruction. however, the mechanism by which s.dysenteriae infection regulates proinflammatory cytokines during intestinal inflammation is still obscure. in this study, we evaluated whether the interaction of β-catenin and nf-κb regulates proinflammatory cytokines tnf-α and il-8 by modulating gsk-3β activity durin ...	2017	28430783
evaluation of pigments from methanolic extract of tagetes erecta and beta vulgaris as antioxidant and antibacterial agent.	the total phenolic content (tpc), antioxidant and antibacterial activities of methanolic extract of marigold flower (mf) (tagetes erecta) and beet root (br) (beta vulgaris) were examined. the present work reveals that mf contained greater amount of tpc (42.5 mg/g gae) as compared to br (39.4 mg/g gae). methanolic extract of mf exhibited excellent dpph free radical scavenging power (ic50 0.0716 mg/ml) and reducing power at 1 mg/ml concentration. similar results have been obtained in ftc and tba m ...	2017	28494614
in vitro antibacterial activity of poly (amidoamine)-g7 dendrimer.	nano-scale dendrimers are synthetic macromolecules that frequently used in medical and health field. traditional anibiotics are induce bacterial resistence so there is an urgent need for novel antibacterial drug invention. in the present study seventh generation poly (amidoamine) (pamam-g7) dendrimer was synthesized and its antibacterial activities were evaluated against representative gram- negative and gram-positive bacteria.	2017	28583153
protection against shigellosis caused by shigella dysenteriae serotype 4 in guinea pigs using escherichia albertii dm104 as a live vaccine candidate strain.	recently, we reported the induction of protective immunity by environmental escherichia albertii strain dm104 against shigella dysenteriae in guinea pig model. in this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum igg and mucosal iga antibody titers. protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs aga ...	2017	28597684
characterization of hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: optimization based statistical modeling.	in this study, the extracting parameters of crude polysaccharides (cpss) from the hypericum perforatum (hp) including extraction time (eti, 60-180min), extraction temperature (ete, 60-90°c), and the water/solid ratio (w/s, 10-30 was optimized by using three-variable-three-level box-behnken design-response surface methodology (bbd-rsm) based on the single-factor experiments. the optimal extraction conditions were as follow: eti 117.5min, ete 74.28°c, and w/s 20.3:1. under these conditions, the ex ...	2017	28602988
dissecting binding of a β-barrel membrane protein by phage display.	membrane proteins (mps) constitute a third of all proteomes, and contribute to a myriad of cellular functions including intercellular communication, nutrient transport and energy generation. for example, tonb-dependent transporters (tbdts) in the outer membrane of gram-negative bacteria play an essential role transporting iron and other nutrients into the bacterial cell. the inherently hydrophobic surfaces of mps complicates protein expression, purification, and characterization. thus, dissectin ...	2017	28627567
characterization of a large antibiotic resistance plasmid found in enteropathogenic escherichia coli strain b171 and its relatedness to plasmids of diverse e. coli and shigella strains.	enteropathogenic escherichia coli (epec) is a leading cause of severe infantile diarrhea in developing countries. previous research has focused on the diversity of the epec virulence plasmid, whereas less is known regarding the genetic content and distribution of antibiotic resistance plasmids carried by epec. a previous study demonstrated that in addition to the virulence plasmid, reference epec strain b171 harbors a second, larger plasmid that confers antibiotic resistance. to further understa ...	2017	28674052
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.	simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.	2017	28713514
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.	a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...	2017	28720951