the effects of the carboxyl-terminus amino acids of the shiga toxin b-subunit on retrograde transport.the shiga toxin b-subunit (stxb), from the enteric pathogen, shigella dysenteriae, is responsible for the attachment of its receptor, globotriaosylceramide (gb3), and navigates the retrograde pathway from the plasma membrane to the endoplasmic reticulum (er). in this study, in order to demonstrate the role of carboxyl-terminus (c-terminus/al) amino acids of the b-fragment on the retrograde transport speed and the retrograde transport pathway, stxb was modified by site-directed mutagenesis and by ...201222552548
cephalosporin-resistant shigella flexneri over 9 years (2001-09) in determine the pattern and antimicrobial resistance genes of cephalosporin resistance in shigella flexneri and shigella dysenteriae over 9 years.201222410619
differential expression of gastric muc5ac in colonic epithelial cells: tff3-wired il1 β/akt crosstalk-induced mucosal immune response against shigella dysenteriae understanding of the signaling mechanism(s) that regulate the differential expression of gastric mucin muc5ac in colonic epithelial cells would contribute significantly to investigations of its role in colonic mucosa infected with the bacterial pathogen shigella dysenteriae. here we show that s. dysenteriae-sinduced expression of interleukin-1β upregulates muc2 expression and the differential expression of muc5ac. differential expression of muc5ac involves crosstalk between interleukin-1β and ...201222389405
comparison of microcalorimetric fingerprint profiles of lonicerae japonicae flos and lonicerae compare the microcalorimetric fingerprint profiles of lonicerae japonicae flos (lj.f) and lonicerae flos (l.f), microcalormietry was applied to find the heat change regularity of bacillus shigae (b. shigae) metabolism affected by lj.f and l.f (we choose lonicera macranthoides hand.-mazz in this paper) with different concentrations. the thermogenic curves and thermodynamics parameters were investigated as evaluation index, and then the date of experiment was studied by similarity analysis. all ...201122242460
antimicrobial effects of psidium guajava extract as one mechanism of its antidiarrhoeal action.a morphine-like spasmolytic action (not naloxone reversible; involving the inhibition of acetylcholine release) and also effects on the transmural transport of electrolytes (na(+) and k(+)) and water have been reported as possible modes of the antidiarrhoeal action of polar fractions of psidium guajava leaf extractives. the objective for this study was to verify if the reported modes of the antidiarrhoeal action should be broadened to include direct antimicrobial actions on some of the more comm ...199922589684
hemolytic-uremic syndrome in asir region.hemolytic-uremic syndrome (hus) is a worldwide disease and a common cause of acute renal failure in childhood. recently the number of cases in our area seems to be increasing.199623008549
subcellular localization of ipac, an invasion plasmid antigen of shigella dysenteriae type 1 and its in-vitro binding capability to mammalian cells.invasion plasmid antigen c (ipac), a 45-kda protein encoded by an invasion plasmid of shigella, is associated with the invasion of epithelial cells by the bacteria. invasive strains of s. dysenteriae type 1 secreted more proteins into the extracellular environment than a non-invasive strain and secreted more ipac protein. an anti-ipac mouse monoclonal antibody was used as a probe to determine the subcellular localization of ipac and its involvement in invasion of mammalian cells. immunogold labe ...199524414917
regulation of cell-wall morphology and growth encoded by plasmids in shigella dysenteriae type 1.shigella dysenteriae type 1, isolated locally, was found to contain six plasmids and these could be eliminated using sds. a 70-kb plasmid was necessary to maintain the normal cell-wall morphology. synthesis of nine major membrane proteins (90 to 40 kda) was severely impaired in all-plasmid cured strains. electron microscopy revealed a prominent separation between the outer and inner membranes of the cured strains, indicating that plasmid loss led to defects in the cell envelope. the growth rates ...199524414647
[effect of combined antibiotics of the tetracycline series with bacteriophage on shigella dysenteriae]. 201424544824
[stability of changed properties of streptomycin-resistant strain of shigella flexneri after prolonged preservation]. 201424544446
[biomycin and levomycethin in prevention and therapy of experimental dysenterial intoxication]. 201424544304
[intramuscular administration of biomycin and terramycin in experimental dysenterial infection]. 201424544302
[effect of bacteriophage on resistance of flexner's bacillus to antibiotics]. 201624544299
[fermentative characteristics of collection of culture of shigella dysenteriae newcastle]. 195324543449
[duration of survival time of shigella dysenteriae in feces]. 195324543444
[antibacterial effect of tjiliwung river water on shigella shigae in connection with the presence of corresponding antagonists and bacteriophages]. 195024539386
in vitro antibacterial activity of poly (amidoamine)-g7 dendrimer.nano-scale dendrimers are synthetic macromolecules that frequently used in medical and health field. traditional anibiotics are induce bacterial resistence so there is an urgent need for novel antibacterial drug invention. in the present study seventh generation poly (amidoamine) (pamam-g7) dendrimer was synthesized and its antibacterial activities were evaluated against representative gram- negative and gram-positive bacteria.201728583153
evaluation of pigments from methanolic extract of tagetes erecta and beta vulgaris as antioxidant and antibacterial agent.the total phenolic content (tpc), antioxidant and antibacterial activities of methanolic extract of marigold flower (mf) (tagetes erecta) and beet root (br) (beta vulgaris) were examined. the present work reveals that mf contained greater amount of tpc (42.5 mg/g gae) as compared to br (39.4 mg/g gae). methanolic extract of mf exhibited excellent dpph free radical scavenging power (ic50 0.0716 mg/ml) and reducing power at 1 mg/ml concentration. similar results have been obtained in ftc and tba m ...201728494614
isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india.a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ...201728720951
detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization.simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses.201728713514
characterization of hypericum perforatum polysaccharides with antioxidant and antimicrobial activities: optimization based statistical this study, the extracting parameters of crude polysaccharides (cpss) from the hypericum perforatum (hp) including extraction time (eti, 60-180min), extraction temperature (ete, 60-90°c), and the water/solid ratio (w/s, 10-30 was optimized by using three-variable-three-level box-behnken design-response surface methodology (bbd-rsm) based on the single-factor experiments. the optimal extraction conditions were as follow: eti 117.5min, ete 74.28°c, and w/s 20.3:1. under these conditions, the ex ...201728602988
protection against shigellosis caused by shigella dysenteriae serotype 4 in guinea pigs using escherichia albertii dm104 as a live vaccine candidate strain.recently, we reported the induction of protective immunity by environmental escherichia albertii strain dm104 against shigella dysenteriae in guinea pig model. in this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum igg and mucosal iga antibody titers. protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs aga ...201728597684
characterization of a large antibiotic resistance plasmid found in enteropathogenic escherichia coli strain b171 and its relatedness to plasmids of diverse e. coli and shigella strains.enteropathogenic escherichia coli (epec) is a leading cause of severe infantile diarrhea in developing countries. previous research has focused on the diversity of the epec virulence plasmid, whereas less is known regarding the genetic content and distribution of antibiotic resistance plasmids carried by epec. a previous study demonstrated that in addition to the virulence plasmid, reference epec strain b171 harbors a second, larger plasmid that confers antibiotic resistance. to further understa ...201728674052
dissecting binding of a β-barrel membrane protein by phage display.membrane proteins (mps) constitute a third of all proteomes, and contribute to a myriad of cellular functions including intercellular communication, nutrient transport and energy generation. for example, tonb-dependent transporters (tbdts) in the outer membrane of gram-negative bacteria play an essential role transporting iron and other nutrients into the bacterial cell. the inherently hydrophobic surfaces of mps complicates protein expression, purification, and characterization. thus, dissectin ...201728627567
shigella dysenteriae infection activates proinflammatory response through β-catenin/nf-κb signaling pathway.shigella dysenteriae (s.dysenteriae) the causative agent of bacillary dysentery invades the human colonic epithelium resulting in severe intestinal inflammatory response and epithelial destruction. however, the mechanism by which s.dysenteriae infection regulates proinflammatory cytokines during intestinal inflammation is still obscure. in this study, we evaluated whether the interaction of β-catenin and nf-κb regulates proinflammatory cytokines tnf-α and il-8 by modulating gsk-3β activity durin ...201728430783
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