intestinal secretion. 1977190182
cyclic adenosine 3', 5'-monophosphate in cerebrospinal fluid during thermoregulation and fever.1. samples of cerebrospinal fluid (c.s.f.) have been taken from the cisterna magna of unanaesthetized cats, whilst rectal temperature was recorded, during exposure of the animals to various ambient temperatures and during fever induced by pyrogen. the concentration of adenosine 3', 5'-monophosphate (cyclic amp) in samples of c.s.f. has been assayed. 2. cats exposed to low ambient temperatures (-2 to +2 degrees c) for 3 h maintained body temperature by both behavioural and autonomic heat gain act ...1976190383
subcellular distribution of steroids in rat adrenocortical tissue after incubation in vitro [proceedings]. 1977194992
depletion of replenishment of cellular cyclic adenosine monophosphate in hemorrhagic shock.hemorrhagic shock in rats was produced by bleeding them to a mean arterial blood pressure of 40 millimeters of mercury, which was maintained for two hours. after sacrifice, small pieces of liver, kidney, muscle and brain were quickly removed and frozen in liquid nitrogen. a protein-free extract of tissues was prepared, and cyclic adenosine monophosphate was measured by the radioimmunoassay procedure. analysis os liver, kidney, muscle and brain showed that there were significant reductions in cyc ...1977201041
cell culture propagation of porcine rotavirus (reovirus-like agent).two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ...1977201198
differential labelling of alpha and beta-noradrenergic receptors in calf cerebellum membranes with 3h-adrenaline. 1977201857
[lipoproteins as serum inhibitors in rubella hemagglutination inhibition test and their elimination (author's transl)].lipoproteins of 20 human sera were isolated by flotation centrifugation and tested for inhibitory activity in rubella hi-test. low density lipoproteins (ldl) showed the highest titers with an average of 1:238,0, whereas the others (vldl, hdl) had lower titers (see tab. 2). the greatest reduction of all classes of lipoproteins was achieved with kaolin treatment. heparin-manganous chloride and dextran sulfate-calcium chloride method mainly reduced the ldl (84.4 resp. 78.7%). in a comparative test ...1977202112
ocular herpes simplex infection. a clinical evaluation of virus isolation and studies on iodo-deoxyuridine resistance.fifty-seven patients with ocular herpes simplex (hs) infection were studied for evaluation of existing methods for virus isolation and its application in diagnosis of hs infection. virus was isolated in 90% of 34 cases with keratitis dendritica, when specimens were taken within eight days of onset of symptoms. the same isolation frequency was obtained in 10 cases of palpebral herpes with conjunctivitis. no isolation was possible in 11 cases of keratitis disciformis. laboratory confirmation was o ...1977202139
the distribution of the inherited hl 1 and r 67 antigens on rabbit serum high density lipoprotein (hdl) particles.qualitative and quantitative analysis of the distribution of hl 1 antigen and r 67 antigen on high density lipoprotein (hdl) particles were conducted on six rabbit sera. the hl 1 antigen showed non-identity with a-i-containing hdl particles, and antiserum to hl 1 did not remove a-i-containing hdl particles. the r 67 antigen showed partial identity with a-i-containing hdl particles. antiserum to r 67 antigen removed 30--40% of a-i-containing hdl particles from the sera of animals which were heter ...1978205613
tumorigenic action of streptozotocin on the pancreas and kidney in male wistar rats.pancreatic islet cell tumors were induced in 38 of 44 male wistar rats (86%), which survived 9 to 14 months following the various treatment schedules. a single i.v. injection of streptozotocin alone, 30, 40, 50, or 65 mg/kg of body weight produced adenomas of pancreatic islet cells in 8 of 9 (89%), 6 of 7 (86%), 2 of 4 (50%), and 1 of 2 rats (50%), respectively. the neoplasms were seen in all of the 8 rats given a single i.p. injection of picolinamide, 250 mg/kg of body weight, 15 min before a s ...1978207424
synthesis and secretion of transferrin by cultured mouse hepatoma cells.the mouse hepatoma cell (hepa-1) in tissue culture has been shown to synthesize and secrete three electrophoretically distinct transferrins. each of these forms of transferrin has a molecular weight of 77,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the concentration of each form is indicated by its staining intensity, which is highest in the form with the fastest mobility and lowest in the form with the slowest mobility. the relative rate of transferrin synth ...1978207612
measuring interhemispheric transfer time in man. 1977210552
selective antagonism by d-alpha-aminoadipate of amino acid and synaptic excitation of cat spinal neurons. 1978210880
supersensitivity to the cyclic gmp response to glutamate during cerebellar maturation. 1978211431
[blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus].in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ...1979219399
identification of cytomegalovirus in bone marrow biopsy.the bone marrow trephine biopsy has become a common procedure for evaluating hematopoietic abnormalities. occasionally, the first clue to an infectious process is obtained from this source. a bone marrow biopsy, done to evaluate pancytopenia in a patient who had received a renal transplant, revealed large intranuclear inclusions diagnostic of cytomegalovirus. antibody titers were initially negative but rose over the next few weeks to confirm the diagnosis. even though the bone marrow biopsy may ...1979219548
demonstration of enzootic bovine leucosis virus in britain. 1978219581
adenosine and deoxyadenosine kinase from rat liver. 1979232628
intracellular oxygen tension and energy metabolism in the cat brain cortex during haemorrhagic shock.the changes in intracellular oxygen tension and energy metabolism of the cat brain cortex were studied by surface fluororeflectometry during haemorrhagic shock. the results may be summarized as follows. (a) intracellular oxygen tension, i.e. the maximum cortical nad reduction obtained during nitrogen gas inhalation decreased gradually during the hypovolaemic phase of shock and finally, the brain cortex became ischaemic. (b) partial uncoupling of the cerebrocortical mitochondrial respiration and ...1979232967
[isolation of bovine cell lines and susceptibility to foot-and-mouth disease virus].morphology and chromosomes of cell sublines derived from two new bovine kidney lines are reported. cell susceptibility to the foot-and-mouth disease virus is discussed. one of the sublines showed epithelial-like cells, while the remainder, elonged fibroblastic-like cells. most of them had a diploid number of chromosomes. from these sublines only one was sensible to the foot-and-mouth disease virus.1979233200
[circadian rhythm of poly a polymerase in rat liver nuclei].isolated nuclei from the liver of rats maintained under daily scheduled conditions of light (12 hrs) and food (8 hrs) showed a peak of mn2+-dependent poly (a) polymerase activity 6 hrs after the beginning of the feeding period, as shown by dna dependent rna polymerase b. in fasting animals the peak of poly (a) polymerase occurs at 18.00.1979233201
the pathogenesis of shigella diarrhea. v. relationship of shiga enterotoxin, neurotoxin, and cytotoxin.the biological activity of the enterotoxin of shigella dysenteriae 1 was compared with that of a well-studied 20-year-old partially purified preparation of neurotoxin from the same organism. enterotoxicity, neurotoxicity, and cytotoxicity were present to an equivalent extent in both preparations. human convalescent antisera and experimental rabbit antisera had equal toxin-neutralizing antibody to the cytotoxic activity in these toxin preparations. multiple protein bands were present in each toxi ...1975236346
inhibition of pathogenic enteric bacteria by hyperbaric oxygen: enhanced antibacterial activity in the absence of carbon dioxide.the antibacterial effects of 24-h exposures to high-pressure oxygen in relation to environmental co(2) were studied at 3 atm absolute (ata) and at 1 ata. eight gram-negative, aerobic and facultatively aerobic, pathogenic enteric bacteria (salmonella typhosa, salmonella paratyphi, salmonella schottmuelleri, shigella dysenteriae, shigella flexneri, proteus vulgaris, pseudomonas aeruginosa, and escherichia coli) were exposed as shallow-broth cultures and agar surface cultures. although broths suppl ...1975238466
regional anesthesia in cattle. 1979260529
a survey of caseous lymphadenitis and its etiology in goats in the united states. 1979260549
[experience with the large-scale manufacture of a live associated mumps-measles vaccine]. 1979262713
[study of the lipid constituents of yeasts cultivated on n-paraffins (toprina and liquipron). methods for their determination in tissues and biological fluids of animals fed such yeasts]. 1979262949
x-linked mental retardation: transmission of the trait by an apparently unaffected male.a pedigree is presented in which an apparently unaffected man transmitted the gene for x-linked mental retardation to at least four of his 12 daughters. none of his 12 sons was mentally retarded. these findings may be explained by a somatic mutation and germinal mosaicism in the father or by a half chromatid mutation in maternal gametes.1978263439
in vitro strength of the human periodontal ligament.the tensile properties of the human periodontal ligament were studied by direct loading and compression experiments. it was found that the ligament behaved in an elastic manner up to an elastic limit after which rupture occurred. the values obtained for the tensile strength of the ligament are in general agreement with those of other workers for similar tissues.1977264865
surgical adjuvant therapy for potentially curable cancer: the rationale of adjuvant chemotherapy and anticoagulant therapy. 1977273400
immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory t cells. 1977302274
binding of 3h-dexamethasone and 3h-corticosterone to rat liver cytosol during postnatal period.the binding of 3h-dexamethasone (3h-dx) and 3h-corticosterone (3h-b) to specific binding proteins of rat liver cytosol was compared during pre- and postnatal period. the binding of 3h-dx was almost twice as high as that of 3h-b during the whole postnatal period. the scatchard analysis showed a number of binding sites for 3h-dx to be 4.44 x 10(-13) mol mg-1 (kd 5.9 x 10(-9) mol 1(-1)) and for 3h-b to be 2.7 x 10(-13) mol mg-1 (kd 1.6 x 10(-8) mol 1(-1)). a negative relationship between the bindin ...1978305844
amino acid transport in thymic- and spleen-derived lymphocytes.we have examined the transport of amino acids by the sodium-dependent "a" and "asc" system in thymic- and splenic-derived lymphocytes from the long-evans rat. lymphocytes derived from the thymus transport amino acids by both the "a" and "asc" systems, whereas lymphocytes from the spleen transport amino acids by the "asc" system only. thymic lymphocytes are capable of establishing a steady state distribution ratio of 7.9 for 2-aminoisobutyric acid, but splenic lymphocytes can attain only 3.5. the ...1978308950
synthesis and analgesic-antiinflammatory activity of some 4- and 5-substituted heteroarylsalicylic acids.we have made a series of 4- and 5-aryl- and 4- and 5-heteroarylsalicylic acid derivatives with the objective of reducing gastric irritation and increasing potency. here we describe a series of 4- and 5-heterocyclic salicylic acids and their antiinflammatory-analgesic potencies measured in comparison to aspirin. an improvement of the therapeutic index over aspirin of 100 was achieved; however, the heterocyclic salicylic acids lacked antipyretic activity. some physicochemical parameters which may ...1978309948
acute epiglottitis: 26 years' experience at the montreal children's hospital.two hundred and four cases of acute epiglottitis treated at the montreal children's hospital between 1951 and 1978 are reviewed. the comparison of tracheostomy vs. nasotracheal intubation is discussed. nasotracheal intubation has been found to be the treatment of choice as long as the necessary services are available in the hospital.1979313456
formation of a stable complex between human proelastase 2 and human alpha 1-protease inhibitor. 1979313930
the general pharmacology of a novel antitussive compound ru 20201.1,2,3,4,4a,9b-hexahydro-8,9b-dimethyl-4[3-(4-methyl piperazin-1-yl)propionamido] dibenzofuran-3-one dihydrochloride (ru 20201) is an antitussive compound with a potency comparable to that of codeine. unlike codeine, ru 20201 is devoid of any apparent cns effects and does not depress the respiratory system. it has no adverse effect on the gastrointestinal tract no has it any analgesic or anti-inflammatory properties. only at very high doses was a local anaesthetic effect observed.1979314807
refractory pneumocystis carinii infection in chronic granulomatous disease: successful treatment with granulocytes.pneumocystis carinii pneumonia developed in an 11-year-old girl with chronic granulomatous disease who had normal cellular and humoral immunity. the patient remained febrile during treatment with sulfamethoxazole-trimethoprim and pentamidine but became afebrile when treated with a series of 12 granulocyte transfusions combined with sulfamethoxazole-trimethoprim. in addition to documenting p carinii infection in chronic granulomatous disease our findings suggest that granulocyte transfusions may ...1979316128
experimental use of a gastric flap on an omental pedicle to close defects in the trachea, pharynx, or cervical esophagus.the use of local or regional skin flaps for repairs after head and neck cancer surgery often increases the deformity of the patient. we present a new procedure using a gastric flap on an omental pedicle. it has been successful in dog experiments where we transferred it to the head and neck region, to serve as a substitute for the standard skin flaps.1977320615
interaction between rifampicin and trimethoprim in vitro and in experimental infections. 1977321415
bacterial toxins as virulence factors: shiga bacillus dysentery viewed as a toxinosis. 1977321946
[genetics and biochemistry of the bacterial ribosome]. 1977322729
biological properties of shigella flexneri 2a toxin and its serological relationship to shigella dysenteriae 1 toxin.a toxin extracted from heat-inactivated, alkaline-treated shigella flexneri 2a showed biological properties similar to those of shigella dysenteria 1 toxin. the s, flexneri 2a toxin was lethal to mice, enterotoxic for ileal loops of rabbits, and cytotoxic for hela cells. a serological relationship between s. flexneri 2a and s. dysenteriae 1 toxin was shown with cross neutralization tests.1977323142
the pathogenesis of shigella diarrhea. vi. toxin and antitoxin in shigella flexneri and shigella sonnei infections in humans.two strains of shigella flexneri and one of shigella sonnei were studied for toxin production in vitro. all of the three strains produced a cell-free cytotoxin that showed marked similarity to that produced by shigella dysenteriae 1. each toxin eluted in two distinct peaks on chromatography with sephadex g-150, was destroyed by heating at 90 c for 30 min, and was neutralized by s. dysenteriae 1 antitoxin. patients with infections due to s. flexneri and s. sonnei developed antibody that neutraliz ...1977323376
characterization of an r-plasmid associated with ampicillin resistance in shigella dysenteriae type 1 isolated from epidemics.ampicillin-resistant strains of shigella dysenteriae type 1 isolated in epidemics in mexico, central america, and bangla desh were examined for the presence of plasmid deoxyribonucleic acid (dna) by gel electrophoresis. all strains contained a heterogeneous population of plasmids. transfer experiments to escherichia coli k-12 indicated that the ampicillin resistance determinant (ap(r)) was located on a 5.5-megadalton (mdal) plasmid identical in all shiga strains examined, as judged by dna hybrid ...1977324394
inhibition of small-intestinal sugar and amino acid transport by the enterotoxin of shigella dysenteriae i.the enterotoxin of shigella dysenteriae i produces fluid and electrolyte secretion in the rabbit ileum. these present studies were designed to evaluate nonelectrolyte transport in rabbit ileal mucosa exposed to shigella enterotoxin. both 10 mm galactose and 5 mm l-alanine absorptions were significantly impaired in enterotoxin-exposed ileal mucosa compared with control mucosa. l-alanine influx was not imparied in two other secretory processes: that induced by cholera enterotoxin and hyperosmolari ...1977324910
pathogenesis of shigella diarrhea. vii. evidence for a cell membrane toxin receptor involving beta1 leads to 4-linked n-acetyl-d-glucosamine oligomers.the binding of shigeua dysenteriae 1 cytotoxin to hela cells in culture and to isolated rat liver cell membranes was studied by means of an indirect consumption assay of toxicity from the medium, or by determination of cytotoxicity to the hela cell monolayer. both liver cell membranes and hela cells removed toxicity from the medium during incubation, in contrast to wi-38 and y-1 mouse adrenal tumor cells, both of which neither bound nor were affected by the toxin. uptake of toxin was directly re ...1977327017
studies on a new antibiotic--chuangxinmycin.a new antibiotic, designated chuangxinmycin and produced by a new species of microorganism actinoplanes tsinanensis n. sp., has been isolated in crystalline form. assay methods, fermentation and isolation procedures for the antibiotic are described. uv-ir-, nmr- and mass spectra as well as specific rotation of the antibiotic are given. its molecular formula is c12h11no2s. studies on its chemical structure and total synthesis have been completed. according to its chemical structure, chuangxinmyci ...1977327539
complete structure of the repeating unit of the o-specific polysaccharide chain of shigella dysenteriae type 3 lipopolysaccharide. 1977328145
somatic antigens of shigella. structural investigation on the o-specific polysaccharide chain of shigella dysenteriae type-2 lipopolysaccharide.the o-specific polysaccharide obtained from shigella dysenteriae type-2 lipopolysaccharide by mild acid hydrolysis consisted of n-acetylgalactosamine, n-acetylglucosamine, d-galactose, d-glucose, and o-acetyl group in the ratio of 2:1:1:1:1. a number of oligosaccharides were obtained by deamination of the n-deacetylated polysaccharide and by smith degradation of the both native and o-deacetylated polysaccharides. the identification of oligosaccharides along with methylation analysis and chromic ...1977330162
[shigella shige as an etiological factor of bacillary dysentery]. 1977330362
shigellosis control--a rosy future? 1977333037
shigella surveillance in the united states, 1975. 1977333038
algorithms in the diagnosis and management of exotic diseases. xxvii. shigellosis. 1977333040
some effects of intravenous prostaglandin e, and endotoxin in young chickens.1 the effects of intravenous prostaglandin e1 and endotoxin were studied in young chickens (11-17 days old). 2 at a thermoneutral ambient temperature (31 degrees c), intravenous prostaglandin e1, produced behavioural and electrocortical sleep, increased oxygen consumption and, after an initial fall, elevated body temperature. below thermoneutrality (16 degrees c), the initial hypothermic effect was more marked and oxygen consumption was lowered. 3 the soporific actions of prostaglandin e1 were s ...1977334307
some effects of prostaglandins e1 and e2 and of endotoxin injected into the hypothalamus of young chicks: dissociation between endotoxin fever and the effects of prostaglandins.prostaglandins e1 and e2 elevated body temperature of young chicks when injected into the hypothalamus at thermoneutrality (31 degrees c). in contrast, they lowered body temperature when so injected below thermoneutrality (16degreesc): the relation of the fall in body temperature to increased heat loss and decreased heat production was examined. 2 the above effects below thermoneutrality were potentiated by pretreatment with inhibitors of prostaglandin synthetase and possible reasons for this po ...1977334308
somatic antigens of shigella. the strucuture of the specific polysaccharide chain of shigella dysenteriae type 5 lipopolysaccharide.the specific polysaccharide was released from shigella dysenteriae type 5 lipopolysaccharide by mild acidic hydrolysis and then purified by gel chromatography on sephadex g-50. the polysaccharide was built up of residues of d-mannose, 2-acetamido-2-deoxy-d-glucose, 3-0-(d-1-carboxyethyl)-l-rhamnose (rhamnolactylic acid) and 0-acetyl groups in a ratio 2:1:1:1. on the basis of radiospectroscopy, methylation analysis, smith degradation, and chromium trioxide oxidation, the repeating oligosaccharide ...1977334537
cell-wall lipopolysaccharide of the 'shigella-like' escherichia coli 058. structure of the polysaccharide chain.two lipopolysaccharide preparations were obtained from escherichia coli 058 by extraction with 45% aqueous phenol and fractional precipitation with cetyltrimethyl ammonium bromide (cetavlon). chemical analysis and polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate showed that the two preparations differed only in the extent of the o-specific polysaccharide moiety. the o-specific polysaccharide was characterized with proton magnetic resonance and infrared spectroscopy, op ...1977334542
location of new clear plaque genes on the p1 map. 1977335644
effects of methylxanthine drugs on pyrogen-induced hyperthermia.the hyperthermic response to pyrogen was not potentiated by caffeine or theophylline administered i.p. into cats or rabbit. injection of these drugs into the anterior hypothalamus or into the third cerebral ventricle in cats was also without effect on pyrexia. these results support the hypothesis that cyclic amp in the anterior hypothalamus does not mediate pyrogen-induced hyperthermia in cats.1977336383
toxin from the culture filtrate of shigella dysenteriae that causes morphological changes in chinese hamster ovary cells and is distinct from the neurotoxin.a toxin that causes morphological changes in chinese hamster ovary cells was isolated from a culture filtrate of shigella dysenteriae 1. it was partially purified by successive column chromatography on diethylaminoethyl-cellulose, bio-gel a-5m, and hydroxylapatite. the toxin was separated from neurotoxin by diethylaminoethyl-cellulose column chromatography and was not lethal for mice.1977336549
[characteristics of the ultrastructural changes of the large intestine mucosa after acute dysentery].electron microscopic examinations of biopsy specimens of the large intestine from 8 patients one month after termination of the acute phase of dysentery revealed dystrophic and destructive changes in surface epithelium cells consisting in the lack of glycocalyx, fragmentation of microvilli, vacuolation and subsequent homogenization of the cytoplasm. swelling and destruction of mitochondria, pyknosis and rhexisof the nucleus, exposure and loss of the intactness of the apical membrane of the cells ...1977337939
rose spots in shigellosis caused by shigella dysenteriae type 1 infection. 1977338095
an overview of recent experiences with plasmid-mediated antibiotic resistance or induced virulence in bacterial diseases. 1977340443
[an epidemic of bacillary dysentery caused by shigella dysenteriae, type 1, in mogadiscio].an epidemic of bacillary dysentery broke out at mogadiscio in the first months of 1976 produced by shigella dysenteriae type 1 supplanting all other familiers of shigella before present in the zone. the date are relative to 675 cases with 27 deceased. the hydrological origin of epidemy was not probable.1977341817
[study of the antigenic relationship between shigella and leptospira. i. antigenic relationship between shigella dysenteriae, type 1 and leptospira sejroe, leptospira hardjo and leptospira sao paulo]. 1977341818
single-dose tetracycline therapy for shigellosis in adults.forty-two adults who had shigella isolated from stool (26 symptomatic, 16 asymptomatic) received a single oral dose of tetracycline hydrochloride (2.5 g). minimal inhibitory concentrations of the 42 isolates demonstrated that 41% were sensitive to tetracycline. sixteen of 18 patients with diarrhea who had tetracycline-resistant shigella and all eight patients with diarrhea who had tetracycline-sensitive shigella were clinical well and had shigella-negative stools 48 hours after therapy. fifteen ...1978342737
antipyretics and fever induced in adult fowls by prostaglandins e1, e2 and o somatic antigen. 1978347328
haemolytic-uraemic syndrome complicating shigella dystentery in south indian children.shigella dysentery caused 65% of all cases of acute renal failure (arf) seen in children treated at the christian medical college hospital, vellore, during the 33 months ending september 1977. in the 40 children with arf secondary to shigella dysentery, haematological findings suggested that they were suffering from the haemolytic-uraemic syndrome, and glomerular hypercellularity and fibrin deposition were present in all 12 patients whose renal histology could be studied. peritoneal dialysis was ...1978350345
cerebellar afferent projections from the vestibular nuclei in the cat: an experimental study with the method of retrograde axonal transport of horseradish peroxidase.details of cerebellar afferent projections from the vestibular nuclei were investigated by the method of retrograde axonal transport of horseradish peroxidase (hrp) in the cat. the distribution of labeled cells in the vestibular nuclei following hrp injections in various parts of the cerebellum indicates that vestibular neurons in the medial and descending nuclei and cell groups f and x project bilaterally to the entire cerebellar vermis, the flocculus, the fastigal nucleus and the anterior and ...1978350598
altered membrane-associated functions in chronic lymphocytic leukemia cells.peripheral blood lymphocytes consisting mainly of neoplastic b cells from patients with chronic lymphocytic leukemia (cll cells) showed a markedly reduced response to the human b-cell mitogens anti-beta2 microglobulin, sepharose-bound protein a and sepharose-bound anti-human immunoglobulin (anti f(ab')2) in all of nine patients studied. on the other hand, cll cells from three out of eight patients tested responded well to the calcium ionophore a23187. sepharose-bound protein a and anti-beta2 mic ...1978350779
[conditions and factors determining the etiological structure of dysentery and its changes. report 1. epidemiological theory of etiological selectivity of the main (primary) pathways of infection and their differences in various nosological forms of dysentery].the authors present the elaborated and formed epidemiological theory ("conformity theory") according to which the etiological structure of dysentery is determined by the etiological selectivity of the main (primary) waves of transmission of the infection differing in various nosological forms of dysentery. in grigoriev-shiga dysentery the domestic way of the spread of infection plays the main role, in flexner and newcastle dysentery--the water way, and in sonne dysentery--the food way (particula ...1978352042
a new approach for a pyrogen test [proceedings]. 1978352465
effects of certain cations (fe, zn, mg, and ca) on bacterial endotoxins.the natural occurrence of cations fe, zn, mg, and ca in the lipopolysaccharide (lps) of both the s and r forms of shigella dysenteriae 1 was studied. lps preparations were obtained either by phenol-water extraction (according to the method of westphal et al., z. naturforsch. 7b:148-155, 1952) or by extraction of cells with hypertonic sodium chloride-sodium citrate (according to the method of raynaud and digeon, c. r. acad. sci. (paris) 229:564-566, 1949), with subsequent chromatographic purifica ...1978357292
interventions in diarrheas of infants and young prevent diarrhea, efforts must be directed toward decreasing environmental contamination of enteropathogens, altering exposure of persons to naturally occurring agents, or to increasing host tolerance to prevalent enteropathogens. a vaccine is feasible in the control of certain enteric infections among high risk groups: typhoid fever and cholera, in areas of hyperendemicity or during an epidemic, shigellosis in institutionalized populations that are at risk to develop illness due to known pre ...1978359524
surface structures of escherichia coli that produce diarrhea by a variety of enteropathic mechanisms.strains of escherichia coli, mostly of human origin, were obtained from several different investigators who had isolated them from patients with diarrhea from many different parts of the world. the mechanisms by which these e. coli were thought to have caused diarrhea included: (i) synthesis of labile, stable, or shigella dysenteriae-like enterotoxins; (ii) invasion of the intestinal mucosa; and (iii) unknown. each strain was carefully examined for pili or flagella to correlate the presence or a ...1978361579
naturally occurring promoter down mutation: nucleotide sequence of the trp promoter/operator/leader region of shigella dysenteriae 16.the promoter/operator/leader region of the trp operon of shigella dysenteriae 16 has single base pair differences from the corresponding region of escherichia coli at positions -24 and -13. the difference at -13 was shown to be responsible for the 90% reduction in promoter function characteristic of the trp operon of s. dysenteriae. the base pair difference at position -13 also renders the operator partially constitutive. this allows the organism to maintain relatively high repressed levels of t ...1978364484
a comparative evaluation of media in the isolation of shigella dysenteriae type 1. 1978365721
mitomycin c stimulates production of a toxin in shigella species that causes morphological changes in chinese hamster ovary cells.shigella dysenteriae produced a toxin that caused morphological changes in chinese hamster ovary cells which was different from the neurotoxin (y. takeda, k. okamoto, and t. miwatani, infect. immun. 18:546--548, 1977). it was found that the toxin was produced not only by s. dysenteriae but also by s. flexneri, s. boydii, and s. sonnei. the production of the toxin was stimulated by the addition of 1 microgram of mitomycin c per ml to the culture in the early logarithmic growth phase.1979370009
characteristics of lipid a-protein complex from endotoxin of shigella dysenteriae type 1 (s and r strains).mild acetic acid hydrolysis of endotoxin (lipopolysaccharide-protein complex) of shigella dysenteriae type 1 (s and r forms) yielded a lipid a-protein complex that consisted of amino acids, fatty acids, and sugar and, in terms of chemical composition, displayed no marked differences between the s and r forms. its protein portion (53 to 56%) consisted of at least 16 amino acids. in the fatty acid portion (14 to 18%), myristic, 3-hydroxymyristic, palmitic, and stearic acids accounted for 50%. the ...1979370018
[nomenclature of the bacteria of the genus shigella and their systematic position in the family of enterobacteriaceae]. 1978371309
construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pl promoter.two multiple-copy, cole1-type, plasmid cloning vehicles, phub2 and phub4, have been constructed that carry four different single restriction sites down-stream from the phage lambda promoter pl. the promoting activity of pl is switched off at low temperature in the presence of a cits gene that specifies a temperature-sensitive repressor but could be activated by heat induction. cits was located either on the host chromosome, or on a second plasmid prk248 that is compatible with the cloning vehicl ...1979372049
shigella vaginitis: report of 38 patients and review of the literature.thirty-eight cases of shigella vaginitis were identified in a retrospective review of records kept over the past 14 years. the cases of vaginitis were due to three subgroups of shigella. vaginitis varied in severity and duration, persisting for several months in some instances. in 47% of the cases, there was associated bloody vaginal discharge. only two children had diarrhea temporally associated with vaginitis, but six others had had diarrhea. many treatment modalities were used. systemic antib ...1979375177
shigella serotypes in recent isolations at lucknow. 1979376439
infectious mononucleosis associated with fatal beta hemolytic streptococcal infection. 1979378507
high-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. melting curves and relaxation a continuation of our studies on structural effects on the 31p chemical shifts of nucleic acids, we present 31p nmr spectra of yeast phenylalanine trna in the presence and absence of mg2+. superconducting field (146 mhz) and 32-mhz 31p nmr spectra reveal approximately 15 nonhelical diester signals spread over approximately 7 ppm besides the downfield terminal 3'-phosphate monoester. in the presence of 10 mm mg2+, most scattered and main cluster signals do not shift between 22--66 degrees c, t ...1979383146
artificial salmonella vaccines: o-antigenic oligosaccharide-protein conjugates induce protection against infection with salmonella typhimurium.outbred mice were vaccinated with various artificial salmonella vaccines and subsequently challenged intraperitoneally with graded doses of virulent salmonella typhimurium. the salmonella vaccines used were: (i) octasaccharide, obtained by hydrolysis of the o-antigenic polysaccharide chain of s. typhimurium strain sh 4809 with phage p22-associated endo-rhamnosidase and covalently linked to either diphtheria toxin or edestine; (ii) purified outer membrane proteins (porins) from s. typhimurium; an ...1979387597
isolation of haemin-requiring mutants of escherichia coli k12.fifty-five haemin-requiring mutants were isolated from haemin-permeable mutants. according to their growth responses to haem precursors and their patterns of porphyrin accumulation, the 55 mutants fell into three groups which were judged to have defects in 5-aminolaevulinate dehydratase, ferrochelatase, and uroporphyrinogen iii cosynthase or uroporphyrinogen decarboxylase. in mutants of the group deficient in 5-aminolaevulinate dehydratase, the mutations were adjacent to lac, and evidence is pre ...1979387909
epidemiology of shigella dysenteriae, type 1 infections, in dacca urban area.a prospective study of 47 families with an index case of shigella dysenteriae type 1 was conducted in the urban areas of dacca. the subsequent infection rates in contacts type 1 were 30.6% and 28.3% in the age groups 0--4 and 5--9. total secondary infection rate was 20.4% with rates in males higher than in females. among the contacts 12.5% developed diarrhoea caused by the same serotype, but 22.1% developed diarrhoea with undiagnosed causes. for one hospitalised case there were seven symptomatic ...1979388775
shigella dysenteriae, sh flexneri, and sh boydii infections in england and wales: the importance of foreign travel. 1979389342
a satire on the influenza of 1803. 1979390272
fifty years ago. paper published in the december issue 1925. a review of a recent epidemic of bacillary dysentery with special reference to a new therapy: a paper read before the uganda branch of the british medical association, march 1925. 1979391538
patterns of shigella species and serotypes in east africa. 1979391541
sub-populations of peripheral lymphocytes from patients with shigella bacillary dysentery with associated this study we ascertained the proportion and absolute number of t, b and nul cell peripheral lymphocytes from shigella dysenteriae type i patients and compared these results with those from age matched controls. single blood samples were collected from 13 bangladeshi children who complained of symptoms for one or more weeks. patients were divided into two categories, with and without a leukaemoid response. the leukaemoid response was primarily granulocytic in nature, but there was a substanti ...1979395727
septicaemic manifestations of shigellosis. 1979396236
shigella dysenteriae 1-like cytotoxic enterotoxins produced by salmonella strains.a salmonella enteritidis strain produced a cytotoxin in addition to heat-labile (lt) and heat-stable (st) enterotoxins. two strains of serotypes salmonella kapemba and salmonella thompson were lt and st negative, but exhibited a cytotoxic effect. after sephadex g-100 fractionation of the crude s. enteritidis material, some high and low molecular fractions had both cytotonic and cytotoxic activities. of the two other salmonellae, only some high molecular fractions contained the cytotoxic substanc ...1979396765
enterotoxins of enteric bacteria--a review.a review of the enterotoxins of enteric bacteria was compiled, taking into account recent developments of the field as well as results of the author's own research work. after characterization of enterotoxins of different origin, their pathogenic role is described, with special attention to the genetic background of these agents. the possible use of enterotoxins for active immunization purposes is also discussed.1979399581
synergy and rifampicin. 1977405361
immunization with shigella dysenteriae type 1: evaluation of antitoxic immunity in prevention of experimental disease in rhesus monkeys (macaca mulatta).the role of serum antitoxic antibody in protection against the dysentery caused by shigella dysenteriae type 1 (shiga's bacillus) was studied in monkeys fed 10(10) virulent organisms after parenteral immunization with a formalin-inactivated shiga toxoid preparation standardized in mice. two 125-microgram doses of toxoid adsorbed on aluminum hydroxide adjuvant and given 14 days apart provided mice with a 54-fold increase in resistance to parenteral toxin. in rhesus monkeys (macaca mulatta), the s ...1977409782
effect of some bacterial products on platelet electrophoretic mobility (pem).bacterial lps, used in our experiments, are able to decrease pem at concentration of 100 microgram/ml after incubation with 1 x 10(7) platelets/ml for 30 min at 37 degrees c. such effect is statistically significant for bacteroides fragilis lps, s. enteritidis and s. typhi. e. coli lps 026:b6 w and e. coli 055:b5 w are not effective at all, s. minnesota (incomplete) lps 595 and 345 free of 0-polysaccharide and partially lacking core in their molecule did not influence pem, while s. minnesota lps ...1977410424
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