formylation of initiator trna methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression.eucaryotic and procaryotic organisms differ in two aspects of their translation machinery: polycistronic messengers are expressed as a sequence of individual proteins only in procaryotes, and the initiation of protein synthesis proceeds with an initiator trna which is found to be modified (formylated) in procaryotes and not in eucaryotes. in the present study, we show that formylation is required in vivo for the coordinate expression of the escherichia coli lactose operon. our experiments are co ...197898518
altered mrna metabolism in ribonuclease iii-deficient strains of escherichia coli.the metabolism of mrna from the lactose (lac) operon of escherichia coli has been studied in ribonuclease (rnase) iii-deficient strains (rnc-105). the induction lag for beta-galactosidase from the first gene was twice as long, and enzyme synthesis was reduced 10-fold in one such mutant compared with its isogenic rnc+ sister; in the original mutant strain ab301-105, synthesis of beta-galactosidase was not even detectable, although transduction analysis revealed the presence of a normal lac operon ...197898520
a.d.a. recommendations to dhew on proposed changes in standards for long-term care facilities. 197898553
observations on acute gastric dilatation in nonhuman the years 1967-1977 we diagnosed 23 cases of acute gastric dilatation in monkeys. fourteen of these animals were macaca mulatta, five macaca fascicularis, and one each of macaca nemestrina, aotus trivirgatus, saimiri sciureus, and colobus guereza. fourteen of the animals were males, nine were females, and all were adults or subadults. mortality was 78% (18 of 23 animals). thirteen of the animals had received on anesthetic, immobilizing, or tranquilizing drug 1-2 days before developing acute g ...197898666
localised insertion of new cell wall in bacillus subtilis. 197898715
electron-microscopic identification of axonal initial segments belonging to interneurons in the dorsal lateral geniculate nucleus of the monkey. 197898733
reduced hepatic bilirubin uridine diphosphate glucuronyl transferase and uridine diphosphate glucose dehydrogenase activity in the human fetus.hepatic bilirubin uridine diphosphate glucuronyl transferase (udpg-t) activity was 0.14 and 0.22 units in two fetuses aged 17 and 22 weeks, respectively, and less than 0.1 unit in 15 fetuses, aged 8--19 weeks compared to 0.68--1.99 units in 21 normal adults. hepatic uridine diphosphate glucose dehydrogenase (udpg-d) activity in 14 fetuses, aged 8--18 weeks, ranged from 6.2--15.0 units (mean = 11.3 +/- 0.7) compared to 28.8--49.2 units (mean = 39.6 +/- 2.5) in eight normal adults (p less than 0.0 ...197898749
[some aspects relating to the aflatoxin generation during the self-heating of cereals].in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...197898933
age-associated changes in the blood picture of the goat. 197898936
improved safety of splenoportography by plugging of the needle tract.splenoportography, a useful preoperative diagnostic procedure in patients with portal hypertension, has been largely discontinued because of the risk of hemorrhage. we have used both ivalon and gelfoam in particle and plug form to plug the needle tract after splenoportography. initial studies were conducted on six dogs. plugging of the needle tract by injection of suspensions of small gelfoam or ivalon particles resulted in intravasation of these particles into the splenic vein. this was demonst ...197898987
microradiographic demonstration of pyelolymphatic backflow in the porcine kidney.pyelolymphatic backflow occurs when intrapelvic pressure rises acutely. although peripelvic and hilar lymphatics have occasionally been demonstrated during intravenous urography and retrograde pyelography, the patterns of intrarenal microlymphatic filling have not previously been studied radiographically in multipapillary kidneys. microradiography was performed after retrograde ureteral injections of barium sulfate suspension in recently excised pig kidneys. contrast filled the ducts of bellini ...197898998
jaundice and breast-feeding among alaskan eskimo newborns.the course, incidence, and severity of neonatal jaundice was studied in 95 alaskan eskimo infants. breast-fed infants had higher bilirubin concentrations than bottle-fed babies. both groups experienced high bilirubin levels, similar to those previously reported in navajo and oriental infants but greater than those observed in whites and blacks. a marked capacity to inhibit hepatic glucuronyl transferase was observed in breast-milk specimens but only partly accounted for the bilirubin differences ...197899026
interferon inducing activity of polyinosinic acid.although poly(i) is generally considered to be inactive as an interferon inducer, we have found several authentic poly(i) preparations to be effective inducers. their interferon inducing ability varied considerably from one cell system to another. in human diploid fibroblasts, primed with interferon and superinduced by cycloheximide and actinomycin d, all active poly(i) samples proved nearly as effective in inducing interferon as poly(i).poly(c). in primary rabbit kidney cell cultures, the activ ...197899486
ultraviolet light-induction and photoreactivation of thymine dimers in a cyanobacterium, anacystis nidulans.partially photoreactivable mutant of anacystis nidulans demonstrates partial photorepair of thymine dimers. the wild type which is completely photoreactivable at the conditions studied shows higher level of thymine dimer photolysis.1978100072
inverse correlation between species lifespan and capacity of cultured fibroblasts to convert benzo(a)pyrene to water-soluble metabolites. 1978101383
purification and characterization of allergens from parietaria officinalis pollen. 1978101439
[salmonellosis]. 1978101617
the surface properties of neisseria gonorrhoeae: topographical distribution of the outer membrane protein antigens.gonococci were labelled with 125i using the lactoperoxidase system. the amount of label incorporated was similar with all strains including those which appeared capsulated. electrophoresis on sodium dodecyl sulphate-polyacrylamide gels revealed that the major proteins labelled were those found in outer membrane preparations. comparison of variants of one strain showed that the major outer membrane protein (protein i) was always present and heavily labelled. the second major protein (protein ii) ...1978102733
effect of cobalt-60 radiation on response to endodontic therapy in monkeys.response of teeth that had received therapeutic doses of cobalt-60 radiation to endodontic therapy were investigated in three monkeys. the results indicated no appreciable effect of the irradiation on the response to root canal treatment aside from reduction in osteoblastic activity.1978103612
toxoplasma gondii: penetration into differentiating friend erythroleukemia cells. 1978103745
shigellosis and escherichia coli diarrhea: relative importance of invasive and toxigenic mechanisms.shigellae and dysentery-like escherichia coli must invade the epithelium of the colon to cause disease which can present as dysentery, diarrhea, or both. this paper addresses the possible role of a shigella dysenteriae-like (shiga-like) toxin in the pathogenesis of shigellosis and e. coli diarrheal diseases. the possibility for such a role is suggested by the following observations: 1) diarrhea, considered to be a result of secretion of water by the small bowel, is frequently observed in shigell ...1979104615
dynamics of corneo-scleral envelope. 1978104926
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
production of antivenom against detoxified taipoxin and immunochemical analysis of the alpha, beta and gamma subunits. 1979105431
food toxins and their implication in human health. 1978105997
[comparative study of methods for the bacterioscopic diagnosis of tuberculosis and of methods for determining drug sensitivity]. 1979106383
[changes in certain biochemical parameters of rat skin following prolonged gamma irradiation after radioprotection]. 1978106409
the role of catecholamines in clinical medicine.the sympathetic nervous system is of major importance for the regulation of several physiological functions. drugs which inhibit the actions of catecholamines and adrenergic drugs are used in the treatment of many clinical disorders. the potential role of catecholamines in a number of human diseases has, however, until recent years been studied to a limited extent only due to lack of methods for quantitation of sympathetic nervous activity. after the development of enzymatic isotope-derivative a ...1979106629
baroreceptor control of atrioventricular conduction in man.although human baroreflexes are known to exert a powerful physiological control on heart rate, little information exists on the physiological control they exert on the atrioventricular conduction system. in 11 normotensive subjects with normal atrioventricular conduction, we altered baroreceptor activity by injection of pressor and depressor drugs (phenylephrine and trinitroglycerin) and recorded mean arterial pressure (map, catheter measurements), r-r interval, and pre-his and post-his interval ...1979106985
alcohol, malnutrition and liver disease. 1979107268
two functional types of neurons in the superficial layers of monkey superior colliculus. 1978107871
spinal cord evaluation by cortical evoked ten monkeys, selective segmental lesions of the dorsal columns at the upper thoracic and middle cervical levels resulted in almost total attenuation of the cortical evoked potential responses to peripheral nerve stimulation. conversely isolated segmental dorsal column preservation showed intact transmission of the evoked responses at rostral spinal cord, nucleus ventralis posterior lateralis, and cortical levels. responses recorded from the intralaminar thalamic nuclei in the region of nucleu ...1979107931
an enzyme-linked immunosorbent assay (elisa) for detecting chicken anti-reovirus antibody at high enzyme-linked immunosorbent assay (elisa) with high sensitivity was developed for use in the avian system. anti-reovirus antibody produced in chickens against the isolants s1133, umi-203, reo-25, and winterfield were detected with this method at high titers (up to 1:14,000).1978107949
immunoelectrophoretic studies on human small intestinal brush border proteins. a quantitative study of brush border enzymes from single small intestinal biopsies.a method for measuring brush border membrane enzymes from small intestinal biopsies by crossed immunoelectrophoresis is presented. the use of a brush border specific antiserum made isolation of the brush border membrane before analysis unnecessary. this prevented loss of material which, together with inactivation of enzymes, was a limiting factor in previous studies of brush border enzymes from peroral biopsies. in 58 biopsies from patients without gastrointestinal disorders a close correlation ...1979108036
multiple patterns of induction of rat liver microsomal mono-oxygenases and other polypeptides by xenobiotics [proceedings]. 1979108146
characteristics of immunoglobulin synthesized early in the development of xenopus laevis. 1979108156
demonstration of human apolipoprotien a in isolated mucosal cells from small intestine and isolated hepatocytes.isolated mucosal cells from the human jejunum and stomach, cryostat sections from the jejunum, isolated parenchymal liver cells and lymphocytes were investigated for the presence of apolipoprotien a (apoa). antisera against purified human apoa-i and apoa-ii were raised in rabbits and conjugated with fluorescein-isothiocyanate (fitc). mucosal cells from jejunum and stomach were isolated with pronase from tissue obtained from operated patients. apoa-i and apoa-ii could be demonstrated in isolated ...1979108182
a solid-phase radioimmunoassay for bacterial lipopolysaccharide.a radioimmunoassay for e. coli 055:b5 lipopolysaccharide (lps) is described. the lps was derivatised by two new methods and subsequently radiolabeled with 125i to a specific activity of 2-4 mci/mg without apparent loss in its biophysical, immunological or biological activities. using antibody-coated polystyrene tubes, a solid-phase radioimmunoassay was developed with a sensitivity of 10-500 ng/ml of lps.1979108200
macaca assamensis as a new host for experimental plasmodium knowlesi infection. 1978108217
preparation and antibacterial activity upon micrococcus luteus of derivatives of iturin a, mycosubtilin and bacillomycin l, antibiotics from bacillus subtilis.methylated and acetylated derivatives of iturin a and mycosubtilin and methylated derivatives of bacillomycin l were prepared and their antibacterial activity on micrococcus luteus was compared with the activity of the original substance. the results obtained show the importance of polar groups for the antibiotic activity of the substances of iturin group.1979108238
gentamycin. 1979108423
immobilization of pyridoxal 5'-phosphate and pyridoxal 5'-phosphate-dependent enzymes on sepharose. 1979108513
[pyruvate and phosphoenolpyruvate carboxylase in methylotrophs].the activity of pyruvate and phosphoenolpyruvate carboxylases was determined in cell extracts of obligate and facultative methylotrophs which metabolized monocarbon reduced compounds via different pathways. phosphoenolpyruvate carboxylase was found to be the only enzyme responsible for the high level of co2 fixation by methylotrophs with the serine pathway (methylosinus trichosporium, hyphomicrobium vulgare, pseudomonas methylica). methylotrophs with the hexulose phosphate pathway mehylobacter c ...1979108526
acquisition and discrimination of appetitively and aversively conditioned heart rate responses in rhesus monkeys.this report describes the acquisition of a conditional heart rate response to both classical aversive and appetitive conditioning in eight rhesus monkeys (macaca mulatta). the behavioral paradigm consisted of two discrete one minute tones followed by the corresponding unconditional stimuli, i.e., electric shock or purina monkey chow. a third tone followed by neither food nor shock served as a discriminative stimulus (ds). the conditional heart rate responses developed in two phases. the first ph ...1978108656
[on the antimicrobial activity of propolis and propolis constituents (author's transl)].after a survey of the literature on the antimicrobial activity of the bee product propolis, the authors discuss their own findings as compared to the chemotherapeutical agents streptomycin, oxytetracycline, chloramphenicol, nystatin, griseofulvin and sulphamerazine. according to the results obtained by testing 25 isolated constituents on bacillus subtilis, staphylococcus aureus, candida albicans and trichophyton mentagrophytes, the antimicrobial properties of this mixture of natural substances a ...1979108687
patterns of virus-immune t-cell responsiveness. comparison of (h-2k x h-2b) leads to h-2b radiation chimeras and negatively selected h-2b lymphocytes.negatively selected h-2k(b)d(b) tdl can be induced to respond strongly to vaccinia virus presented in the context of both h-2k(k) and h-2d(b) when stimulated in irradiated h-2k(k)d(b) recipients. addition of excess (h- 2k(k)d(b) x h-2k(b)d(b))f1 tdl, which are low responders to h-2d(b)-vaccinia virus, does not obviously suppress the reactivity pattern of the h-2k(b)d(b) t cells. however, lymphocytes from chimeras made by reconstituting h- 2k(b)d(b) mice with (h-2k(k)d(k) x h-2k(b)d(b))f(l) bone ...1979115959
annotations on the measurement of liver ferritin, especially in the rat. 1979120687
effects of renal nerves on renal hemodynamics. i. direct stimulation and carotid occlusion.renal nerves were stimulated either directly (loop electrodes) or indirectly (bilateral carotid occlusion with maintenance of control blood pressure) in anesthetized dogs to determine the effects on renal blood flow (rbf), glomerular filtration rate (gfr), sodium excretion (unav), and intrarenal distribution of blood flow (microsphere method). direct nerve stimulation decreased rbf 20% in 9 of 10 dogs; but gfr, unav, and intrarenal distribution of blood flow did not change significantly. carotid ...1975124020
[possible causes determining the developmental rhythm of populations of the nasopharyngeal gadfly of sheep, oestrus ovis l. (diptera, oestridae)].on the basis of observations undertaken in azerbaijan and available literary data the author considers the hypotheses concerning the reasons causing the delay of the ist instar larvae development and determining the age structure changes in population of oestrus ovis l. within a year. the main reasons are as follows: factors depending on the population density at the larval phase, the light day duration within the life cycle of the botfly females.1975124845
behavioral changes following vmh lesions in rats with controlled insulin levels.the effects of ventromedial hypothalamic (vmh) lesions were studied in female rats made diabetic with streptozotocin that were given twice daily injections of protamine zinc insulin (0.75 mu/100g/day) and in non-diabetic animals of the same sex. hyperphagia resulted from vmh lesions in both diabetic animals whose insulin levels were controlled and in non-diabetic animals. all animals with lesions exhibited persistent increases in feeding during the light protion of the light-dark cycle. signific ...1975125424
effect of methysergide, a blocker of serotonin receptors, on plasma prolactin levels in lactating and ovariectomized rats.the effect of methysergide (mes, 2.5 mg/100 g body wt), a serotonin antagonist, on prolactin release has been studied in lactating and ovariectomized rats. mes caused significant increases in prolactin release in both animals. studies in ovariectomized, hypophysectomized rats indicate that this effect is not due to a decrease in the peripheral metabolism of prolactin. in vitro incubations of anterior pituitary fragments with mes failed to demonstrate any increase in prolactin release, suggesting ...1975126860
characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa.neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ...1975126993
the hurler syndrome: treatment of cultured hurler fibroblasts with normal human serum.prolonged replacement of fetal calf serum by normal human serum for the enrichment of medium during tissue culture of hurler fibroblasts resulted in increased acid mucopolysaccharides in the cells and in the medium. the predominant intracellular mucopolysaccharide had the characteristics of dermatan sulfate when hurler cells were treated with either serum. normal human serum contains a nonspecific coreective factor capable of augmenting the loss of 35so4-amps from hurler cells, but not from norm ...1975127180
[first izolation of enterobacterium a 12 (sachs) in bratislava in the year 1971 (author's transl)]. 1975127664
scattered mitoses in mature joint cartilage in rabbits after local trauma. a chalone effect?this is a demonstration of local traumatization of articular cartilage inducing mitoses in parts of a joint other than the site of inquiry. parts of one of the patellar cartilages of the full-grown rabbit were scored while the other knee was subjected only to arthrotomy. radioactive thymidine and autoradiographic technique demonstrated that labeled chondrocytes appeared not only around the score in the patella but also scattered in the tibia and femoral condyles. whether the mechanism of regiona ...1975127681
immunologic relationship among fibrinolysins secreted by cultured mammalian tumor cells. 1975127717
identification and actions of gastric inhibitory polypeptide. 1975128084
in vitro fertilisation of human oöcytes.this paper describes attempts to cure infertility due to irremediable fallopian tube lesions by the laparoscopic recovery of pre-ovulatory ovarian oöcytes, their fertilisation in vitro, and their subsequent reimplantation into the uterine cavity.1975128140
[changes in the lungs and the mass of the right ventricle of the heart in chronic hypoxia (experimental-morphological study)].in experiments on 105 white rats using histological, histochemical, and morphometric methods the state of the lungs following daily "rises" in a pressure chamber to the "hight" of 5000--9000 m at verious time intervals--from 1 day to 9 weeks was studied. in 18 animals "ascents" were preceded by sinistral pulmonectomy. in parallel, the heart was investigated by the method of separate weighing. at initial stages changes in the lungs were characterized predominantly by impairment of the blood- and ...1975128341
the effect of butacaine on adenine nucleotide binding and translocation in rat liver mitochondria.the effect of the local anaesthetic, butacaine, on adenine nucleotide binding and translocation in rat liver mitochondria partially depleted of their adenine nucleotide content was investigated. the range of butacaine concentrations that inhibit adenine nucleotide translocation and the extent of the inhibition are similar to the values obtained for native mitochondria. butacaine does not alter either the total number of atractyloside-sensitive binding sites of depleted mitochondria, or the affin ...1975128352
[organization of the veterinary service of a dairy complex]. 1975129913
[epidemiological study of shigella dysenteriae 1 in 2 communities of the guanacaste province, costa rica, 1975]. 1976136972
effect of different albumin media on the lipid-mobilizing action of sympathicotropic substances in vitro.the authors studied the effect of adrenotropic substances on lipolysis in rat epididymal adipose tissue in albumin medium in vitro. on using albumins of different origin (human, bovine), the pd2 values for catecholamines differed by more than one order, in correlation to the type of albumin used. the isopropylnorsynephrine pd2 values did not differ. the addition of ascorbic acid (100 microng/ml) raised the catecholamine pd2 values and completely equalized the pd2 values found in both media. the ...1977140390
acid mucopolysaccharides in metal-induced tumor and connective tissue capsules surrounding implants in rats. 1975142824
[toxicological characteristics of thaliblastine[].the toxic action of the antiphlogistic drug thaliblastin, containing 93-95 per cent of the alcaloid thalicarpine was investigated. with its single intraperitoneal injection to mice the dl10 of the drug amounts to 273-306 mg/kg, dl50-325-350 mg/kg and dl90-370-402 mg/kg. with its 5-day long administration the respective figures for mice are 102-165, 125-201 and 154-243 mg/kg and for rats--170, 203 and 272 mg/kg. the respective figures for a 20-day long introduction to mice are 155, 185 and 220 mg ...1977143373
cardiac hypertrophy and antihypertensive therapy.biochemical (myocardial dna, rna, and hydroxyproline) and humoral (plasma [pra] and kidney [kra] renin activity) factors were determined in spontaneously hypertensive rats (shr) and normotensive wistar controls (nr) before and following treatment with minoxidil or propranolol. minoxidil (150 mg.litre-1 drinking water) effectively controlled blood pressure (17.3 kpa vs 24.9 kpa [130 mmhg vs 187 mmhg], p less than 0.001) despite marked and sustained increases in both pra and kra ventricular weight ...1977145319
changes in immunological activity of rat lymphoid organs during pregnancy.immune reactivity in lymhoid organs of rats during the last week of syngeneic (lewis: l x l) or allogeneic (females lewis x males wistar: l x w) pregnancy was compared with that found in non-pregnant animals. the thymus weight was slightly reduced and the response of thymic lymphocytes to phytohaemagglutinin and concanavalin a was slightly, but not significantly, elevated in pregnant rats. by contrast, the response of lymphoid cells from spleen and mesenteric lymph nodes to the mitogens was redu ...1978149623
the multicomposite structure of tendon.a revised morphological model for the crimp structure of tendon is presented. the 300-500 mu diameter tendons of the mature rat tail are comprised of from one to more than ten substructures, called fascicles, of 80-320 mu diameter. fascicles each possess a "crimp structure" demonstrable in the polarizing microscope and neighboring fascicles within a tendon usually exhibit crimp registry. the fascicle itself is shown to be a cylindrical array of planar-zig-zag crimped 500-5000 a diameter collagen ...1978149646
[method for simultaneous study of peristaltic and pendular movements of isolated rabbit ileum].a method is described for studying simultaneously the peristalsis and the pendular movements in the isolated pieces of rabbit ileum. at the same time, using this method it is possible to analyse the actions of drugs on the mechanisms which ensure the pendular movements and the propulsive activity, as well as their relationship. the method is relatively simple and only classical and commercially available equipments are needed. with this method the records and the volume of fluid expelled are sui ...1978150905
replacement of endoplasm with artificial media in plasmodial strands of physarum polycephalum. effects on contractility and morphology. 1978151630
immunological methods employed in an attempt to induce erythema nodosum leprosum (enl) in the mouse foot pad model five different parameters were employed to simulate the condition of enl as observed in the human. the experimental groups with five to six months leprosy infection were injected intravenously with various anti-mycobacterial antibodies, m. leprae sonicate and 'b' cells obtained from syngenic donors. the control group of animals, infected similarly, were treated either with m. leprae sonicate or gamma globulins precipitated from normal human serum. all recipients were ...1978151764
phage typing of nontypable isolates of staphylococcus aureus using the new phages 94, 95, and 96 and the heat-shock treatment.of 2,144 isolates of staphylococcus aureus (from human beings in new york state) received in the authors' laboratory during 1975, 992 (46.,%) were nontypable with the use of the international basic set of bacteriophages. these 2,144 isolates were retested with the use of the recently added phages 94, 95, and 96. nearly 60% of the isolates were lysed with one or more of these three phages, including 64% of those previously nontypable, thus reducing the percentage of nontypables to 16.5%. one hund ...1978152059
[bilboquet's osteosynthesis in open fractures and compound injuries of the hand]. 1978154714
activation of suppressor t cells in human autologous mixed lymphocyte of autologous t and mitomycin-c treated b cells results in increased dna synthesis in the responding t cells. t cells thus activated in amlc exerted suppressive effects on both the proliferative and cytotoxic responses of fresh unstimulated t cells to allogeneic cells in mlc. the suppressor cells generated are sensitive to treatment with mitomycin-c. amlc-activated cells treated with mitomycin-c failed to suppress both cytotoxicity and proliferation in fresh primary mlc. it appears th ...1979156235
histopathogenesis of spontaneous periodontal disease in conventional rats. i. histometric and histologic study. 1979161576
classification of enterotoxins on the basis of activity in cell culture.two cell culture systems were used in a study of the biological properties of several bacterial enterotoxins in vitro. by means of one model, in which hela cell monolayers were used, cytotoxic effects, interms of detachment of cells from a glass surface due to cell death, were assayed. by means of the second model, activation of the adenyl cyclase-cyclic adenosine 3', 5'-monophosphate (amp) system, in terms of increased steroidogenesis by y-1 adrenal cells (an effect which we have termed cytoton ...1975162926
cyclic 3',5'-amp phosphodiesterase of rabbit aorta.cyclic amp and cyclic gmp phosphodiesterase activities (3' : 5'-cyclic amp 5'-nucleotidohydrolase, ec were demonstrated in the isolated intima, media, and adventitia of rabbit aorta. the activity for cyclic amp hydrolysis in the intima was 2.7-fold higher than that for cyclic gmp hydrolysis. the activity for cyclic amp hydrolysis in the media was approximately equal to that for cyclic gmp hydrolysis, but in the adventitia, cyclic gmp hydrolytic activity was 2.1-fold higher than cyclic ...1975164219
cyclic adenosine-3',5'-monophosphate in cerebrospinal fluid during fever and antipyresis. 1975166164
template specificity of the cytoplasmic dna polymerase in xenopus laevis oocytes. 1975167780
effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat.the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ...1975169297
characterization of monkey peripheral neutrophil granules during infection.rhesus monkey (macaca mulatta) neutrophils were shown to contain the azurophilic granule maker enzymes myeloperoxidase and beta-glucuronidase but were deficient in the specific granule markers alkaline phosphatase (akp) and lysozyme. isopycnic centrifugation of leukocyte homogenates on linear sucrose gradients resulted in cosedimentation of myeloperoxidase and beta-glucuronidase with an equilibrium density of 1.18. after an intravenous inoculation of monkeys with salmonella typhimurium akp activ ...1975170208
solubilization of the [8-lysine]vasopressin receptor and adenylate cyclase from pig kidney plasma membranes.adenylate cyclase and the [8-lysine]vasopressin receptor were solubilized from pig kidney medulla membranes using the nonionic detergent triton x-100. optimal conditions for solubilization were under continuous stirring in a medium containing 0.5% (/v) triton x-100, 100 mm tris-hcl, ph 8, and 10 mm mgcl2. both adenylate cyclase activity and [3h][8-lysine]vasopressin binding activity were recovered in a -26,000 x g supernatant of detergent-treated membranes. the yield of solubilized adenylate cyc ...1975170274
[characteristics and types of clostridium perfringens derived from various animals]. 1975170433
effect of shigella enterotoxin on electrolyte transport in rabbit ileum.shigella dysenteriae i is one of several bacteria which produces an enterotoxin capable of stimulating intestinal water and electrolyte secretion. unlike cholera and escherichia coli enterotoxins which have been shown to increase intracellular cyclic adenosine monophosphate levels in the small intestine, the mechanism by which shigella enterotoxin causes intestinal secretion is not known. to study shigella enterotoxin-stimulated intestinal secretion, rabbit ileal mucosa exposed in vivo to shigel ...1975172398
ultrastructural myocardial changes produced by viruses.some of the ultrastructural pathological alterations in the myocardium produced by viral infections with members of the picornavirus group are described. these alterations varied from mild to severe, with necrosis. in animals infected with coxsackie b1 virus and emc virus, readily identifiable viral crystals were frequently found in association with the myocardial lesions by means of electron microscopy. the electron microscope made it possible to study vary early myocardial changes which were n ...1975172990
suppression of pulmonary antibacterial activity following sendai virus infection in mice: dependence on virus dose. 1975173264
unrelated animal viruses share receptors. 1976175292
degenerative alterations in the ventral cochlear nucleus of the guinea pig after impulse noise exposure. a preliminary light and electron microscopic study.guinea pigs were exposed to the noise of 40 shots of an alarm pistol held at a distance of about 60 cm. the ventral cochlear nuclei were studied in phase contrast and electron microscopy after both survival periods and longer periods of up to 55 days survival. marked degeneration of primary cochlear nerve endings and of synapting secondary neurons of the posterior caudal part of the ventral cochlear nucleus (avcn) and the octupus cell area (oca) of the posterior ventral cochlear nucleus (pvcn) w ...1975175774
a radiometric method for predicting effectiveness of chemotherapeutic agents in murine leprosy.a simple radiometric method has been developed for evaluating the effect of drugs on the metabolism of m. lepraemurium. the method is based on the measurement of the 14co2 produced through bacterial metabolism of acetate-u-14c. seventeen drugs were tested: bacitracin, cephaloridine, chloramphenicol, cycloserine, dactinomycin, dds, ethionamide, inh, kanamycin, methenamine mandelate, nitrofurantoin, oxacillin, polymyxin b, rifampicin, streptomycin, sulfadimethoxine and vancomycin. the drugs which ...1975176120
[obtaining the beta-globulin fractions from swine and cattle sera and a study of their immunological activity].the rivanol precipitation was used to obtain beta-globulin fractions from specific swine sera against edema disease, paratyph and aujeszky's disease as well as from normal ovine and swine sera. agar electrophoresis revealed that the preparations produced contained beta-globulin (86 per cent), gamma-globulin (5 per cent), and alpha2-globulin (9 per cent). the beta-globulin preparations were studied for the presence of antibodies against e. coli, salmonellae, staphylococci, myxovirus parainfluenza ...1976176771
the oncogenic potential of some avian adenoviruses causing diseases in chickens.the oncogenic potentials of three different strains of avian adenoviruses (the tipton strain of the inclusion body hepatitis virus, the dpi-2, and the indiana c viruses) were investigated in newborn hamsters. animal inoculations were via two routes, subcutaneous and intracerebral. all three viruses proved nononcogenic for the hamsters observed over a period of 225 days. however, lesions of hepatitis similar to those of inclusion body hepatitis of chickens were seen in three hamsters inoculated w ...1976176986
regulation of mitochondrial protein turnover by thyroid hormone(s).1. the effect of thyroidectomy on turnover rates of liver, kidney and brain mitochondrial proteins was examined. 2. in the euthyroid state, liver and kidney mitochondria show a synchronous turnover with all protein components showing more or less identical half-lives compared with the whole mitochondria. the brain mitochondrial proteins show asynchronous turnover, the soluble proteins having shorter half-lives. 3. mitochondrial dna (m-dna) of liver and kidney has half-lives comparable with that ...1975177002
[epidemiology of human and animal brucellosis in western africa. the results of six studies in the ivory coast, upper volta, and nigeria].brucellosis has a two-fold importance in public health: the transmission of the disease to man by contact with animals or ingestion of raw milk is of direct importance; of indirect importance is the loss of animal protein. the authors have carried out ten epidemiological investigations in different bio-climatic zones of west africa. more than 120 villages were evaluated. in each village, 100 persons were chosen at random and all or part of the animal population was also studied. three tests were ...1976177324
evaluation of the antilipolytic action of clomiphene in vitro.clomiphene (10(-3) - 10(-2) m) in a dose-dependent manner inhibited the lypolytic response of isolated rat epididymal adipose tissue and fat cells to epinephrine, acth, and dibutyryl-cyclic amp. furthermore, it reduced the non-hormonally stimulated activity of a crude preparation of lipase from epididymal adipose tissue. the accumulation of cyclic amp produced by epinephrine in fat cells was not prevented by clomiphene at a concentration causing antilipolytic activity. it is concluded from these ...1976177345
pregnancy interrupting effects of some bacterial toxins.embryotoxic properties of shigella dysenteriae and clostridium perfringens toxins, of e. coli endotoxin, v. cholerase and e. coli enterotoxins were compared in mice. e. coli endotoxin has embryotoxic effects at all stages of pregnancy. e. coli enterotoxin v. cholerae enterotoxin and shigella dysenteriae toxin are most effective mainly at earlier stages of pregnancy. clostridium perfringens toxin has no embryotoxic effect.1976178014
activation of intestinal mucosal adenylate cyclase by shigella dysenteriae i enterotoxin.because the mechanism whereby shigella dysenteriae i enterotoxin induces intestinal secretion is unclear, the effect of this toxin on adenylate cyclase activity in rabbit ileal mucosa was studied under various in vitro and in vivo conditions. activation of adenylate cyclase by shigella enterotoxin was observed only when substrate (atp) concentrations above the km of adenylate cyclase were employed. these concentrations of atp are greater than those required to demonstrate activation of adenylate ...1976178569
mechanism of plasma cyclic amp response to hypoglycemia in man.the effect of insulin-induced hypoglycemia on plasma cyclic amp levels was studied in normal volunteers, adrenalectomized, and sympathectomized subjects. significant increases in plasma glucagon were observed in all groups. normal subjects all had two- to threefold rises in plasma camp while no response was seen in any adrenalectomized or sympathectomized subject. these findings suggest that the mechanism for enhanced plasma camp release during insulin-induced hypoglycemia is catecholamine depen ...1976178982
the effect of infectious laryngotracheitis virus on the bursa of fabricius of young chickens. 1976181407
[infection of hela cells by herpes virus of horses type 1 in different temperature and dose of the virus (author's transl)]. 1976183896
monkeypox and whitepox viruses in west and central africa.prospects for the eradication of smallpox are now highly encouraging. with the cessation of man-to-man transmission, the question of possible animal reservoirs of smallpox becomes increasingly important. during the period 1970-1975, 20 cases of a smallpox-like disease were detected in smallpox-free areas of tropical rain forest in west and central africa. epidemiological and virological investigations revealed that the disease was caused by an animal poxvirus termed monkeypox virus, a member of ...1976186209
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