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[antipuretic, antimicrobic action of gegen qinlian tang]. 19873328655
[trial use of embryonal and early-fetal thymus extracts in the treatment of non-healing skin defects. ii. crural ulcers in humans]. 19873329747
xylose lysine desoxycholate agar for recovering shigella dysenteriae 1 and shigella flexneri from faeces. 19873330107
imipenem/cilastatin for pediatric infections in hospitalized patients.one hundred and four children who were hospitalized for documented or suspected non-cns bacterial infections (56 males/48 females, 22 days to 15 years old) were treated with intravenous imipenem/cilastatin for 9.4 days (range 3 to 28 days). children up to three years of age received 100 mg/kg/day and older children 60 mg/kg/day, administered in four divided doses. bacterial pathogens were isolated before therapy in 85%. diagnoses in the 74 evaluable patients included bronchopneumonia with or wit ...19873331043
increase in multiresistance of shigella dysenteriae type 1 strain in rwanda. 19873333152
clinical features, including haemolytic-uraemic syndrome, in shigella dysenteriae type 1 infection in children of rangoon. 19873333769
[non-tuberculous infections of the spine].non tuberculous spondylodiscitis of the rachis is an uncommon entity that affects boys and male adults with greater frequency. the zone with higher incidence of affection is the lumbar region. usually the causal germ travels through the hematogenous via from an extrarachidial infectious area. clinical manifestations begin with an acute segmentary rachialgia and paravertebral muscular contracture while presenting fever. in special cases of little boys or when having radiated pain, the presenting ...19873333891
[non-tuberculous infections of the spine].non tuberculous spondylodiscitis of the rachis is an uncommon entity that affects boys and male adults with greater frequency. the zone with higher incidence of affection is the lumbar region. usually the causal germ travels through the hematogenous via from an extrarachidial infectious area. clinical manifestations begin with an acute segmentary rachialgia and paravertebral muscular contracture while presenting fever. in special cases of little boys or when having radiated pain, the presenting ...19873333891
shiga toxin from shigella dysenteriae 1 inhibits protein synthesis in reticulocyte lysates by inactivation of aminoacyl-trna binding.inhibition of the peptide elongation cycle of eukaryotic protein synthesis by shiga toxin from shigella dysenteriae 1 was examined in toxin-treated reticulocyte lysate mixtures. peptidyl transferase activity of toxin-treated ribosomes was measured by following the decrease in peptidyl-trna concentrations when puromycin was added after incubation with toxin. concentrations of [3h]leucine-labeled peptidyl-trna were measured by extraction with cetyltrimethylammonium bromide. the data suggest that s ...19863334156
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...19883345436
nutritional requirements of shigellae for growth in a minimal medium.most (about 81%) of the clinical isolates of shigellae that were tested failed to grow in a minimal medium. of the auxotrophic isolates belonging to the four shigella species, 98% grew in a minimal medium containing methionine, nicotinic acid, and tryptophan. the combination of methionine and tryptophan appears to be an obligatory requirement for shigella dysenteriae serotype 1 strains, while the combination of nicotinic acid and tryptophan appears to be obligatory for serotype 2. requirements w ...19883346071
variation in the human lymphocyte sister-chromatid exchange frequency: results of a long-term longitudinal study.the variation in lymphocyte sister-chromatid exchange (sce) frequency as a function of time was investigated in nonsmokers and smokers. the smokers were divided into 3 groups depending on their smoking status. the group termed 'smokers' participated in a program to stop smoking but did not reduce or eliminate their use of tobacco; 'smoke enders' successfully completed the smokending program and remained free of tobacco for the duration of the study, while the 'variable' group stopped smoking for ...19883347215
[functioning of inhibitory mechanisms of gastric secretion during variations in its intensity].seasonal and ovulatory fluctuations of "alimentary" gastric secretion were accompanied by synphase changes of its fatty inhibition in dogs with pavlov gastric pouch and with basov fistula of the stomach. secretory variations stimulated with histamine led to no such inhibitory fluctuations. changes in functioning of stimulating mechanisms of the gastric gland seem to be accompanied by parallel changes of functioning of inhibitory ones in natural conditions. an increase or decrease of acid gastric ...19883356274
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...19883366515
[hoof diseases as a result of stall floor defects].the pig's hoof is characterized by sudden changes between hard and soft horn and a high growth rate of the horn wall. these facts contribute to the high incidence of hoof alterations, which is seen in various inadequate floors. hoof alterations are only painful in cases, where the corium is irritated. some hoof alterations are described, the question of poor footing is discussed. the author provides recommendations regarding the recognition of inadequate barn floors and their improvement. stable ...19883368898
cytochrome p-450-catalyzed dehydrogenation of 1,4-dihydropyridines.a variety of different 4-substituted 1,4-dihydropyridine hantzsch esters are substrates for ring dehydrogenation by a cytochrome p-450 (p-450) enzyme (p-450 ut-a); the substitutent could be varied from a hydrogen to a naphthalenyl, but a pyrenyl derivative was not dehydrogenated. when a 4-alkyl group is present, both the p-450 which oxidizes the substrate and other p-450s can be inactivated (by putative alkyl radicals). p-450s did not discriminate with regard to removal of the 4-h atoms from an ...19883372517
[parasitic gastrointestinal nematodes of game ungulates in the belgian ardennes]. 19883375795
[evaluation of the results of performance tests for stallions and the swiss saddle horse shows]. 19883375797
study of humidification potential of a heat and moisture exchanger in tracheotomized dogs.though a heat and moisture exchanger (hme) has been used in clinical practice following a tracheotomy on a limited basis, no work has been done to document the humidification potential of an hme in this situation. eighteen dogs underwent tracheotomy and were killed at either 1 day (nine dogs) or 10 days (nine dogs) postoperatively. following the tracheotomy, the animals were divided into three groups according to the type of humidification received: ambient conditions, warm mist, or hme. the tra ...19883377401
a direct microassay for serum retinol (vitamin a alcohol) by using size-exclusion high-pressure liquid chromatography with fluorescence detection.serum retinol (bound to plasma retinol-binding protein, rbp) can be determined by direct injection of as little as 20 microliter of serum or plasma by using size-exclusion high-pressure liquid chromatography (se-hplc) with fluorescence detection. toyo soda tsk g-3000sw columns (0.75 x 7.5-cm guard column plus 0.75 x 30-cm analytical column) were eluted with 0.2 m nacl/0.01 m phosphate buffer (ph 6.8) at 1 ml/min, with detection at 280 nm for protein elution. fluorescence of the retinol-rbp compl ...19883407933
c-myc expression and transformed phenotypes in hybrid clones between mouse plasmacytoma s194 cells and normal spleen cells or fibroblasts.expression of the rearranged c-myc oncogene and transformed phenotypes was investigated in 2 different types of somatic cell hybrid clones between a balb/c mouse plasmacytoma line (s194) and normal allogeneic spleen cells or fibroblasts. in the parental s194 cells, one allele of the c-myc was rearranged and its 5'-flanking region was partially deleted by recombination with the immunoglobulin c alpha gene. due to this recombination, s194 cells expressed approximately 20-fold higher than normal sp ...19883417371
genetic characterization of leishmania isolates at 37 enzyme loci. 19883417374
knobbed spermatozoa in two normande bulls.two unrelated yearling normande bulls in a bull stud had 26 and 18% knobbed spermatozoa as well as other major sperm abnormalities. one of the bulls was eliminated from the bull stud because of poor semen quality and growth performance. the other bull was maintained for additional semen evaluation, during which time the mean percentage of major sperm abnormalities decreased, indicating amelioration of a transient testicular degeneration.19883417539
clinical trial of low-fat, high-carbohydrate diet in subjects with mammographic dysplasia: report of early outcomes.despite evidence that dietary fat intake may influence breast cancer risk, there is little information about the effects of dietary fat on the human breast. we have studied the effects of dietary fat on the breast by examining the influence of dietary fat reduction on mammographic dysplasia (nodular or sheetlike areas of radiological density). subjects with mammographic dysplasia were randomly allocated to a control group, in which they received advice about maintaining a balanced diet (36% of c ...19883418730
pressure sores: a biochemical test for early detection of tissue damage.this study was undertaken to seek biochemical indicators in blood associated with the onset of early tissue damage which may progress to the formation of a pressure sore. changes in serum creatinine phosphokinase (cpk), inorganic phosphate, and lactate dehydrogenase in systemic blood of pigs were investigated before, during, and after local indentation. pressures of 540mmhg and 700mmhg were applied for six hours on both sides of the scapula and backs of five anesthetized animals. two hours after ...19883421821
disseminated intravascular coagulation in post-dysenteric haemolytic uraemic syndrome.coagulation studies were carried out in 14 children with haemolytic uraemic syndrome that followed acute dysentery. stool cultures showed shigella dysenteriae in 3 cases and were sterile in the remainder. prolongation of the prothrombin time, activated partial thromboplastin time and thrombin time and raised levels of fibrinogen degradation products were found in 12 cases, indicating the presence of disseminated intravascular coagulation. renal histologic examination showed cortical necrosis in ...19873425309
glutathione metabolism in isolated rat hepatocytes: acinar heterogeneity of detoxifying enzymes and effects of ethanol.periportal and perivenous hepatocytes were isolated by the digitonin-collagenase perfusion technique. the activity of the cytosolic glutathione s-transferase was higher in perivenous cells, but the cytosolic glutathione reductase and the microsomal glutathione s-transferase activities were evenly distributed. in contrast, both the se-dependent and the microsomal se-independent glutathione peroxidase activity and the glucose-6-phosphate dehydrogenase activity was much lower in perivenous hepatocy ...19873426686
serological investigations on the cause of diarrhoea on the cape verde islands.in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9.19873443746
serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria.188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus.19873443752
moniliella suaveolens var. nigra. 19873444445
osteogenic sarcoma and soft tissue myxoma in a patient with fibrous dysplasia and hemoglobins jbaltimore and s.a 41-year-old man with recognized polyostotic fibrous dysplasia since late childhood developed fibroblastic osteogenic sarcoma in the left tibia. four months after the initial diagnosis, an intramuscular myxoma was discovered in the left thigh. twenty years previously he had been found to be heterozygous for hemoglobins jbaltimore and s. malignant transformation in fibrous dysplasia is unusual and may be associated in some individuals with prior irradiation. soft tissue myxomas associated with f ...19863456858
actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro.mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ...19873482153
immune responses to h-2kd antigen expressed by recombinant vaccinia virus.a recombinant vaccinia virus (vv-h2kd-6) containing the coding sequence for the murine major histocompatibility complex class i antigen h-2kd has been constructed and used to express h-2kd on the surface of infected cells. vaccinia expressed h-2kd has been shown to generate an h-2kd-specific primary cytotoxic t-cell response in mice infected with the recombinant virus and to stimulate an h-2kd-specific cytotoxic t-cell response in vitro. cells infected with the recombinant virus acted as targets ...19863489941
a case of human b cell leukemia that implicates an autocrine mechanism in the abnormal growth of leu 1 b cells.production of b cell growth factor (bcgf) from b-chronic lymphocytic leukemia (b-cll) cells was demonstrated. freshly isolated monoclonal b-cll cells expressed surface mu, delta, b1, and leu 1, but not ba (an antigen expressed only on activated b cells). upon stimulation with anti-igm, they secreted bcgf, which could act on anti-igm-stimulated autologous leukemic cells as well as anti-igm-stimulated normal b cells. cell lines established from these leukemic cells also constitutively secreted bcg ...19863490495
development of vaccines against shigellosis: memorandum from a who meeting.endemic shigellosis is a worldwide problem, with a high morbidity rate in most developing countries; substantial mortality may also occur, especially with disease caused by shigella dysenteriae serotype 1. the limited efficacy of current measures to control this infection makes the development of vaccines for the prevention of shigellosis particularly important. this memorandum describes the clinical features of and immunity to shigellosis, summarizes the present status of efforts to develop sui ...19873495364
dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen.the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ...19873495738
idiopathic production of interleukin-1 in acquired immune deficiency syndrome.we determined the capacity of peripheral blood monocytes from 19 patients with acquired immune deficiency syndrome (aids) or related conditions (1 with lymphadenopathy, 8 with aids-related complex, and 10 with aids) to produce intracellular and extracellular interleukin-1 (il-1) spontaneously and upon stimulation with bacterial endotoxin. all patients were anti-human t-cell lymphotropic virus type iii antibody positive. results were compared with those obtained with 10 normal controls of similar ...19873498739
production and assay of ovine t cell growth factor by concanavalin a stimulated peripheral blood leukocytes.conditions for the production and assay of ovine t cell growth factor (tcgf) activity were evaluated. peripheral blood leukocytes were stimulated with concanavalin a (con a) in the presence of 2% autologous serum or serum-free media. supernatants were harvested after 30 hr and concentrated for further characterization. a 28 hr proliferation assay with 2.5 x 10(4) 24 hr con a blasts per well was optimal for detection of tcgf. peak tcgf activity occurred with a 30-37 kd molecular weight fraction. ...19873499702
[substantiation of using pancreatic galvanization in the treatment of acute pancreatitis]. 19873502158
dog inactive renin: biochemical characterization and secretion into renal plasma and lymph.it has been suggested that the dog is a useful model for studies of inactive renin (ir). however, the nature and origin of the trypsin-activated angiotensin-forming activity in dog plasma have not been fully defined. we characterized dog ir using renin-specific antibody, immunoaffinity, and affigel blue chromatography. activated ir resembles renal and plasma active renin in biochemical and immunological properties. ir was detected in renal lymph, arterial and venous plasma, as well as renal extr ...19863510560
shigellaemia in adults: case reports and literature review. 19863510864
rectal histology in acute bacillary dysentery.a recent epidemic of acute shigella dysentery in west bengal (india) provided us with an opportunity to examine the rectal mucosal abnormalities seen in this condition. one hundred two patients were investigated using sigmoidoscopy, rectal biopsy, and rectal swab for culture. pure culture of shigella was obtained in 37 cases, and the rectal biopsy specimens from these patients were assessed in detail. the mean (+/- sd) duration of illness was 47.8 +/- 27.4 h (range 8-120 h), and most patients (3 ...19863510937
colonic perforation in shigella dysenteriae 1 infection. 19863511451
ultrastructural pathology of the rectal mucosa in shigella dysentery.colonocyte damage in the rectal mucosa in shigellosis is the result of bacterial invasion and leads to ulceration. additional factors in colonocyte damage may be the shigella cytotoxin and, especially in colonic crypt cells, bacterial endotoxin. a vascular lesion was present in the lamina propria of the rectal mucosa, which resembled endothelial damage secondary to bacterial endotoxins. in patients with longer duration of symptoms, relative vascular insufficiency, activated lymphocytes, eosinoph ...19863515957
[development of the classification of bacteria in the genus shigella]. 19863518304
prevention of traveler's diarrhea by the tablet form of bismuth subsalicylate.in a randomized double-blind study, swiss adults traveling to tropical countries for 12 to 28 days took a solid formulation of bismuth subsalicylate (1.05 or 2.1 g/day on a twice-daily regimen) or placebo. efficacy was evaluated in 231 volunteers. diarrheal incidence was reduced by 41% in persons taking the high dose (p = 0.007) and by 35% in those taking the low dose (p = 0.03) with excellent compliance. no serious adverse reactions occurred, but objectionable taste, constipation, and nausea we ...19863518624
comparative efficacies of single intravenous doses of ceftriaxone and ampicillin for shigellosis in a placebo-controlled trial.to evaluate ceftriaxone for the treatment of shigellosis, 94 adult males with acute dysentery were randomly assigned to receive ceftriaxone (1 g), ampicillin (4 g), or saline placebo intravenously in single doses in a double-blind design. stool cultures were positive for shigella dysenteriae in 52 patients, s. flexneri in 38 patients, and other species in 4 patients. both ceftriaxone and ampicillin caused reductions in the mean duration of fever and the means of daily stool frequency 2 to 4 days ...19863518625
immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique.thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ...19863519753
the relation between production of cytotoxin and clinical features in shigellosis.the relation between in vitro production of hela cell cytotoxin by strains of shigella and clinical symptomatology was determined for 35 travelers from the united states who developed shigellosis in guadalajara, mexico. there were 25 patients with shigella sonnei, eight with shigella flexneri, one with shigella boydii, and one with shigella dysenteriae. these strains were evaluated for in vitro production of cytotoxin. the amount of cytotoxin did not correlate with the number of stools passed, t ...19863519788
pathogenesis of shigella diarrhea. xi. isolation of a shigella toxin-binding glycolipid from rabbit jejunum and hela cells and its identification as globotriaosylceramide.a glycolipid that specifically binds shigella toxin was isolated from both hela cells and rabbit jejunal mucosa and identified as globotriaosylceramide (gb3) by its identical mobility on hptlc to authentic erythrocyte gb3. toxin also bound to a band tentatively identified as alpha-hydroxylated gb3. in addition, toxin bound to p1 antigen present in group b human erythrocyte glycolipid extracts. the common feature of the three binding glycolipids is a terminal gal alpha 1----4gal disaccharide link ...19863519828
two toxin-converting phages from escherichia coli o157:h7 strain 933 encode antigenically distinct toxins with similar biologic activities.escherichia coli o157:h7 strain 933 contains two distinct toxin-converting phages (933j and 933w). the biologic activities and antigenic relationship between the toxins produced by 933j and 933w lysogens of e. coli k-12, as well as the homology of the genes that encode the two toxins, were examined in this study. the 933j and 933w toxins, like shiga toxin produced by shigella dysenteriae type 1, were cytotoxic for the same cell lines, caused paralysis and death in mice, and caused fluid accumula ...19863522426
enzyme-linked immunosorbent assay for shigella toxin.an enzyme-linked immunosorbent assay (elisa) was developed for the detection of shigella toxin. for the assay, a mouse monoclonal antibody against the b subunit of the toxin and a rabbit polyclonal antibody against the holotoxin were employed. the monoclonal antibody was used to coat wells of a microtiter plate, and the polyclonal antibody preparation was used as the detecting antibody. the amount of bound polyclonal antibody was determined by using a goat anti-rabbit immunoglobulin g-alkaline p ...19863522627
comparison of norfloxacin and nalidixic acid for treatment of dysentery caused by shigella dysenteriae type 1 in adults.a severe epidemic of dysentery began late in 1979 in northeast zaire and spread to rwanda, burundi, and tanzania. the epidemic strain is a multiply resistant shigella dysenteriae type 1, which acquired resistance against trimethoprim and more recently against nalidixic acid in the course of the epidemic. a comparative open trial in rwandan adults with shiga dysentery involved 18 patients treated with norfloxacin at 400 mg twice daily and 12 patients treated with nalidixic acid at 1 g three times ...19863524434
specific detection of plasmodium falciparum malaria by a molecularly cloned dna probe.a highly repeated dna sequence from plasmodium falciparum was cloned and used as a probe in molecular hybridization to detect malaria. our results indicate that the probe is specific to p. falciparum but not to other species of plasmodium and is extremely sensitive. as little as a 20 pg parasite dna, which is equivalent to about 1000 parasites can be detected. the cloned dna can be used as a diagnostic tool to follow the course of infection of falciparum malaria.19863527161
bacterial toxins and diarrhoea.bacteria and their toxins are responsible for an enormous burden of diarrhoeal disease. knowledge about the toxins and their mechanisms of action is limited. thus, although considerable information is available about the mechanism of action of cholera toxin and a small number of heat-stable enterotoxins, information on the role and action of many others is incomplete. the demonstration of a toxic effect in a test system does not necessarily imply that that activity is relevant to the pathogenesi ...19863527494
multiply drug-resistant shigella dysenteriae type 1 and travelers' diarrhea. 19863528325
regulation of adenovirus mrna formation. 19863529876
fetal epithelial binding of 1,2-dibromoethane in mice.whole-body autoradiography and computer-assisted image analysis were used to study the tissue binding of the volatile carcinogenic pesticide 1,2-dibromo[14c]ethane (dbe) in c57bl mouse fetuses. autoradiograms obtained from pregnant mice in late gestation (day 16-17), showed a high level of non-volatile metabolites in the epithelia of the fetal upper alimentary tract and respiratory tract. as determined by image analysis, the concentration of non-extractable (presumably covalently bound) metaboli ...19863530523
comparison of dna probes and the sereny test for identification of invasive shigella and escherichia coli strains.forty-two shigella and 29 escherichia coli strains were screened for invasiveness in the sereny test and for hybridization with two recently described dna probes for the invasiveness plasmid. both probes produced identical results. all sereny-positive strains hybridized with both dna probes. three sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.19863531233
an epidemic of bacillary dysentery in western rwanda 1981-1982.the epidemiologic and clinical aspects of a major shigella dysenteriae epidemic resistant to all available drugs that occurred in western rwanda in 1981-82 are analyzed. about 5% of the population was affected. in total, there were 11,801 cases of dysentery, with an average of 833 cases/month. the course of the epidemic was independent from seasonal variations. in children under 5 years of age, the majority (61%) of dysentery cases involved males; at all other ages, however, there was a femal ...19863533269
[prevalence and care of patients with proteus mirabilis enteritis]. 19863533289
[prevalence and care of patients with proteus mirabilis enteritis]. 19863533289
septic pylephlebitis following shigella bacteremia: case report. 19863535150
morphologic evaluation of the effects of shiga toxin and e coli shiga-like toxin on the rabbit intestine.the effects of a shiga toxin derived from shigella dysenteriae type 1, strain 60r, and a shiga-like toxin from the enterohemorrhagic escherichia coli o157:h7, strain 933, were studied in the in vivo rabbit ileal loop model. the effects of both toxins were similar and resulted in severe villus blunting by 18-24 hours after exposure. with both toxins, a dose effect was noted; and the lesions, first detected at 2 hours after inoculation, became more severe over time. both toxins appeared to act dir ...19863535529
shigella dysenteriae type 1 enterocolitis.shigella dysenteriae type 1 is much more virulent than shigella flexneri and sonnei which are endemic in australia. this report describes a 22 year old woman who acquired shigella dysenteriae type 1 whilst travelling in india. during the course of her illness, she developed severe enterocolitis for which a subtotal colectomy was performed. the illness resembled fulminant ulcerative colitis and its infectious nature was difficult to establish because several fecal cultures failed to grow the path ...19863535768
production of shiga toxin and other cytotoxins by serogroups of shigella.we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...19863537154
enzyme-linked immunosorbent assay to detect shiga toxin of shigella dysenteriae and related toxins.a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga toxin. four species of shigella, escherichia coli, and vibro parahaemolyticus were tested for production of shiga or shiga-like toxin by elisa and vero cell bioassay. in the elisa, most strains of s. dysenteriae and some strains of e. coli isolated from traveler's diarrhea were positive. these elisa-positive strains were positive by vero cell bioassay without exception. some e. coli strains and most v. para ...19873539985
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...19863542256
ionic requirements for entry of shiga toxin from shigella dysenteriae 1 into cells.the ionic requirements for entry of shiga toxin into cells were examined by measuring inhibition of protein synthesis after short-term incubations with toxin. the sensitivity of vero cells and hela cells to shiga toxin was strongly dependent on the divalent cation present. vero cells were most sensitive in the presence of cacl2 and srcl2, whereas hela cells were equally sensitive in the presence of mgcl2, srcl2, and cacl2. both cell lines were protected by bacl2, cocl2, and mncl2. inhibitors of ...19873542829
identification of the carbohydrate receptor for shiga toxin produced by shigella dysenteriae type 1.the binding of shiga toxin isolated from the bacterium shigella dysenteriae type 1 to a series of glycolipids and to cells or cell homogenates has been studied. bound toxin was detected using either 125i-labeled toxin or specific monoclonal antibody and 125i-labeled anti-antibody. overlay of toxin on thin-layer chromatograms with separated glycolipids and binding to glycolipids coated in microtiter wells established that the toxin specifically bound to gal alpha 1-4gal beta (galabiose) placed te ...19873543013
outbreak of post-measles gastroenteritis due to shigella dysenteriae type-i in karsog tehsil, district mandi (h.p). 19863543110
shigellosis in bangladesh. 19873543150
convulsions in childhood shigellosis. clinical and laboratory features in 153 children.we studied 153 children who experienced convulsions associated with shigellosis. the male-female ratio was 1.2:1.0. thirty-six children had a previous history of febrile convulsions, and 31 children had a family history of convulsive disorder. most of the children were 0.5 to 3 years of age, although 49 (32%) were older than 3 years of age and 20 (13.1%) were older than 5 years of age. all children were febrile; in 75% of the children, the temperature was over 39 degrees c. the majority of the c ...19873544808
[a preliminary study on the usefulness of the plasmid profile analysis for the epidemiological investigation of shigellosis]. 19863545491
voluntary hyperventilation changes recruitment order of parasternal intercostal motor units.the order of recruitment of single-motor units in parasternal intercostal muscles during inspiration was studied in normal human subjects during quiet breathing and voluntary hyperventilation. electromyograms were recorded from the second and third intercostal spaces by means of bipolar fine wire electrodes. flow at the mouth, volume, end-expired co2, and rib cage and abdominal anterior-posterior diameters were monitored. single-motor units were identified using criteria of amplitude and shape, ...19873558179
[proliferative activity of experimental brain tumors].the method of autoradiography was used to study the parameters of the cell cycle and the intensity of proliferative processes in three transplantable rat brain tumors differing in the duration of the latent period and sensitivity to chemotherapy. the tumors were found to be similar in the duration of the cell cycle and its phases, the mean values of the label index and growth fraction, and were characterized by the irregularity of the proliferative processes within the tumor; the label index was ...19873564792
cytotoxicity of shiga toxin for primary cultures of human colonic and ileal epithelial cells.shiga toxin purified from shigella dysenteriae 1 was cytotoxic to cultured epithelial cells from human colon and ileum. the cytotoxicity, which affected only about 50% of treated cells, was neutralized by rabbit antiserum monospecific for shiga toxin and mediated by protein synthesis inhibition.19873570477
hereditary hyperlipidemia in the rabbit due to overproduction of lipoproteins. i. biochemical studies.an inherited metabolic disorder in a strain of new zealand white rabbits, characterized by marked hypercholesterolemia (394 +/- 100 mg/dl), with moderately elevated or normal triglyceride levels is described. low density lipoprotein (ldl), intermediate density lipoprotein (idl) and very low density lipoprotein (vldl) cholesterol levels were increased. vldl and idl, and to a lesser extent ldl, had increased free cholesterol and esterified cholesterol content, and triglyceride content was reduced. ...19873579720
antibodies to rat liver cathepsins: characterization and use for the identification of enzyme precursors. 19863579871
clinical and biochemical significance of toxin production by aeromonas hydrophila.production of cytotoxin and enterotoxin by aeromonas strains obtained from stools of 50 children in mexico and texas and from blood of 9 children with sepsis was determined. results were correlated with clinical features of infected children as well as with biochemical traits of aeromonas strains. cytotoxin was produced by 40 of 42 aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsi ...19873584426
cardiac-specific troponin-i radioimmunoassay in the diagnosis of acute myocardial infarction.the cardiac isotype of the myofibrillar contractile protein, troponin-i, is located specifically in the mammalian heart. a sensitive radioimmunoassay has been developed to detect human and nonhuman primate cardiac troponin-i in serum down to 10 ng/ml. immunochemical cross reactivity with skeletal troponin-i was only 2% and was species nonspecific. normal patient levels of cardiac troponin-i are about 10 ng/ml. in patients with acute myocardial infarction (n = 32), serum cardiac troponin-i was el ...19873591601
tubal microsurgery. ii. experimental use.tubal microsurgery is a valuable tool to study tubal reproductive physiology and various infertility problems. microsurgical tubal resection reanastomoses of the rabbit fallopian tube and their impact on fertility are reviewed. data obtained from experimental work on the function of cilia and muscle activity in tubal transport are discussed.19873596349
[combined effects of fentanyl and diazepam on hemodynamics and myocardial blood flow in dogs with normal and ischemic heart]. 19873613053
pentapeptide nuclear localization signal in adenovirus e1a.the adenovirus e1a gene products are nuclear proteins important in transcriptional control of viral functions during infection. by producing normal e1a proteins and derivatives of e1a in bacteria and microinjecting these proteins into cultured cells, we were able to examine their ability to localize to the nucleus. we showed that a short peptide sequence at the carboxyl terminus of e1a is necessary for the rapid (30-min) nuclear localization of that protein. additionally, we showed that just the ...19873614197
thermally induced disulfide bond exchanges in human ige.the thermally induced changes in human ige was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. when the ige (1 mg/ml) was heated at 56 degrees c for several hours in the absence of sodium dodecyl sulfate, it gave aggregated forms that could be reduced completely to heavy and light chains with dithiothreitol. on the other hand, in the presence of sodium dodecyl sulfate (0.1%), the ige was changed by heating to five components with small mol. wts dependent on temperat ...19873614214
myocardial dysfunction in sepsis.a synthesis of the postulated sequence of events for development of the inappropriate response of the myocardium to a demand for increased cardiac index is schematically represented in fig. 10. in sepsis, bacteremia and/or the release of endotoxin activate the macrophages which release a spectrum of mediators. some of these mediators (and possibly also endotoxin itself) have a negative influence on myocardial contractile function either directly and/or through altering cellular calcium flux. thi ...19873615452
myocardial dysfunction in sepsis.a synthesis of the postulated sequence of events for development of the inappropriate response of the myocardium to a demand for increased cardiac index is schematically represented in fig. 10. in sepsis, bacteremia and/or the release of endotoxin activate the macrophages which release a spectrum of mediators. some of these mediators (and possibly also endotoxin itself) have a negative influence on myocardial contractile function either directly and/or through altering cellular calcium flux. thi ...19873615452
altered shape and size of red blood cells in obese hyperglycaemic mice.red blood cells (rbcs) from hyperglycaemic ob/ob-mice, normoglycaemic controls, and a healthy man were sucked into a narrow capillary, photographed and measured. mouse rbcs had a smaller diameter than human ones. although of normal diameter, the ob/ob-mouse rbcs exhibited increased area and volume, and more frequently than control rbcs had a minimum cylindrical diameter greater that 3 micron. cross-sectional profiles with minimum bending resistance were computed for rbcs of mean area and volume. ...19873630732
isoperiodic bursting by magnocellular neuroendocrine cells in the rat hypothalamic slice.1. recruitment of magnocellular neuroendocrine cells (m.n.c.s) to a repetitive burst pattern (phasic firing) is associated with increased vasopressin secretion from neurohypophysial terminals in the intact animal. based on invertebrate studies, bursts of action potentials can arise in two distinct ways: as an intrinsic property of the recorded cell or as an emergent property of synaptic interactions. 2. the majority of phasic m.n.c.s in the hypothalamic slice preparation display an endogenous pa ...19873656153
photoperiodic modification of negative and positive feedback effects of oestradiol on lh secretion in ovariectomized goats.ovariectomized shiba goats carrying an oestradiol implant (4-10 pg/ml) were kept under a short-day light regimen (10l:14d; group 1, n = 4) or a long-day regimen (16l:8d; group 2, n = 4). plasma lh concentrations were lower (p less than 0.05) in group 2 than in group 1 between days 40 and 200, suggesting an enhanced negative feedback effect of oestradiol on lh secretion under a long-day regimen. on days 30, 60, 100, 149 and 279, an lh surge was induced by i.v. infusion of oestradiol for 48 h; the ...19873656284
effect of different fractions of dialyzable leukocyte extract--transfer factor--on the chemiluminescence of human phagocytes.the chemiluminescence (cl) inducing effect of the fractions of dialyzable leukocyte extract (dle)--transfer factor--from tonsil leukocytes was tested on the phagocytes of human whole blood. the fractionation of dle by sephadex g-25 results in three main fractions: i, ii, iii. we found only fraction ii having a direct cl inducing effect on the non-stimulated cells. fraction ii increased further the cl of phagocytes which were stimulated by zymosan (mannozym). fractions i and iii were ineffective. ...19873661353
the mode of action of shiga toxin on peptide elongation of eukaryotic protein synthesis.the effect of shiga toxin, from shigella dysenteriae 1, on the component reactions of peptide elongation were investigated. enzymic binding of [3h]phenylalanine-trna to reticulocyte ribosomes was inhibited by 50% at 7 nm toxin. elongation factor 1 (eef-1)-dependent gtpase activity was also inhibited. both reactions were not restored by addition of excess eef-1 protein. in contrast, toxin concentrations of 200 nm were required to inhibit by 50% the elongation factor 2 (eef-2)-dependent translocat ...19873663122
leukemoid reaction in shigella dysenteriae type 1 infection. 19873666850
intra-right-atrial balloon pumping (irabp) during a fontan procedure. 19873676005
[change in tissue glycogen reserves in tumor bearers as a reflection of a hypoglycemic stress syndrome].glycogen content in the brain, liver and skeletal muscles of rats bearing ascite zajdela hepatoma (azh) and solid 27 hepatoma (27-h) has been studied. serum glucose levels directly correlated with liver glycogen reserves. in the terminal stage of tumor growth depletion of liver glycogen was observed, while the stores of muscle glycogen did not diminish. within 1-4 days (azh) and 15-30 days (27-h) after implantation the stores even exceeded those of control healthy rats. in the terminal stage, in ...19873689968
[cytogenetic activity of metabolites of pesticides representing different classes of chemical compounds].the cytogenetic activity of some substances formed in agricultural plants during metabolism of pesticides of four classes of chemical compounds was studied in the culture of human peripheric blood lymphocytes. metabolites were shown either to have mutagenic properties similar to those of the initial compounds (ziramtetramethylthiourea, both being mutagens; captan-phthalimide, both possessing no cytogenetic activity) or to be considerably transformed in comparison with them as a result of deactiv ...19863705167
somatotopic organization of motoneurons innervating the pronators, carpal and digital flexors and forepaw muscles in the dog: a retrograde horseradish peroxidase study.central distribution of motor neurons innervating the individual forearm and forepaw muscle through the median and ulnar nerves was studied in the dog using the retrograde horseradish peroxidase (hrp) method. all hrp-labeled cells were seen ipsilaterally in restricted parts within the dorsolateral (dl) and retrodorsolateral nucleus (rdl) of the ventral horn at levels from the cranial tip of c7 to the cranial third of t2. the results showed that motoneurons situated more cranially supply the more ...19863708348
increased collateralization of the cerebellothalamic pathway following neonatal hemicerebellectomy.the postlesional reorganization of the cerebellothalamic cells was here studied using a multiple retrograde tracing technique. to this purpose, the cerebellothalamic cell population was investigated in 3 groups of adult rats: (a) cases hemicerebellectomized at birth; (b) cases hemicerebellectomized in adulthood; and (c) control unlesioned ones. in all of the groups the fluorescent tracers diamidino yellow and fast blue were injected bilaterally in the thalamus and the retrograde labeling obtaine ...19863708349
triethyltin-induced neuronal damage in neonatally exposed rats.neuropathological and biochemical effects of neonatal exposure to the alkyl metal triethyltin were examined in juvenile male long evans rats. rats were injected intraperitoneally on postnatal day 5 with 6 mg/kg of triethyltin bromide and sampled on day 20. the brains of tin-treated animals weighed significantly less than either saline or starved controls and exhibited a marked caviation of the ventrolateral surfaces. histologically, neuronal necrosis was noted in the entorhinal and transitional ...19863714127
morphogenesis of sclerotome and neural crest in avian embryos. in vivo and in vitro studies on the role of notochordal extracellular material.the distribution of sclerotome and neural crest cells of avian embryos was studied by light and electron microscopy. sclerotome cells radiated from the somites towards the notochord, to occupy the perichordal space. neural crest cells, at least initially, also entered cell-free spaces. at the cranial somitic levels they moved chiefly dorsal to the somites, favouring the rostral part of each somite. these cells did not approach the perichordal space. more caudally (i.e. trunk levels), neural cres ...19863719666
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