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total hip arthroplasty after fracture of the acetabulum. long-term results.we made a retrospective study of 55 primary total hip arthroplasties in 53 patients with a history of previous acetabular fracture. the mean follow-up was 7.5 years and the average age at fracture was 48.7 years. the incidence of radiographic femoral loosening (29.4%), symptomatic loosening (15.7%), and femoral revision (7.8%) were similar to those previously reported at 10 years for routine arthroplasties by stauffer (1982). on the acetabular side, the incidence of radiographic loosening (52.9% ...20072211750
gorham's syndrome (massive osteolysis): a case report. 20042213318
plants used in guatemala for the treatment of gastrointestinal disorders. 1. screening of 84 plants against enterobacteria.gastrointestinal disorders are important causes of morbidity in developing countries. natural healing is the traditional way of treating these diseases in guatemala. ethnobotanical surveys and literature reviews showed that 385 plants from 95 families are used in guatemala for the treatment of gastrointestinal disorders. the activity of 84 of the most commonly used plants was screened in vitro against five enterobacteria pathogenic to man (enteropathogenic escherichia coli, salmonella enteritidi ...19902214824
immunolabelling of shiga toxin in macrophages infected with shigella dysenteriae 1.immunolabelling of shiga toxin in macrophages infected with a non-invasive shigella dysenteriae 1 isolate showed that bacteria remained alive for 3 h after ingestion within the phagocytic vacuole and synthesized shiga toxin. the normal process of toxin secretion was, however, impaired by the phagosomal environment and toxin molecules accumulated within the bacterial cytoplasm.19902218059
hemolysin as a marker for serratia.all serratia marcescens strains (total of 33) of different sources were hemolytic including clinical strains previously classified as being nonhemolytic. dna fragments of the two hemolysin genes hybridized with the chromosomal dna of s. marcescens, s. liquefaciens, s. kiliensis, s. grimesii, s. proteamaculans, s. plymutica, s. rubridaea which were also hemolytic. the restriction pattern of the hemolysin locus differed in each strain. s. ficaria and s. marinorubra expressed a different hemolysin ...19902222120
parathyroid gland disease and hypercalcemia. 20072223448
parathyroid gland disease and hypercalcemia. 20072223448
normal early pregnancy: serum hcg levels and vaginal ultrasonography findings.vaginal sonography and determination of serum hcg levels were carried out in 22 healthy pregnant women every 2 to 4 days after the first positive pregnancy test until a living fetus was observed. gestational age was calculated from the day of ovulation, assessed by lh surge or hcg administration, plus 14 days. a gestational sac of 1-3 mm was detected at a mean (sem) of 31.2 (0.2) days of gestation (range 30-33 days). the corresponding mean hcg level was 730 iu/l (30) and the range 467-935 iu/l ( ...20072223680
normal early pregnancy: serum hcg levels and vaginal ultrasonography findings.vaginal sonography and determination of serum hcg levels were carried out in 22 healthy pregnant women every 2 to 4 days after the first positive pregnancy test until a living fetus was observed. gestational age was calculated from the day of ovulation, assessed by lh surge or hcg administration, plus 14 days. a gestational sac of 1-3 mm was detected at a mean (sem) of 31.2 (0.2) days of gestation (range 30-33 days). the corresponding mean hcg level was 730 iu/l (30) and the range 467-935 iu/l ( ...20072223680
thermodynamic properties of globular proteins and the principle of stabilization of their native structure.a semi-empirical method has been used to estimate the thermodynamic parameters of hydration of buried surface areas of ribonuclease s, lysozyme and myoglobin from the model of complete unfolding according to ooi et al. ((1987) proc. natl. acad. sci. usa 84, 3086-3090). the buried surface area of proteins is considered as the difference between the accessible surface area of native protein and the completely extended polypeptide chain according to lee and richards ((1971) j. mol. biol. 55, 379-40 ...20082223840
differentiation of shiga toxin and vero cytotoxin type 1 genes by polymerase chain reaction.two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction technique to distinguish genes for shiga toxin in shigella dysenteriae 1 and type 1 vero cytotoxin (vt1) in escherichia coli. vt1a and vt1b primers directed at a common 130-base-pair (bp) fragment of the stx and slti genes detected template nucleic acid in both shiga toxin-positive s. dysenteriae 1 and vt1-producing e. coli strains. vt1c and vt1d primers, targeting a 140-bp fragment of the promoter region of t ...19902230244
plasmid profiles of shigella dysenteriae type 1 isolates from ethiopia with special reference to r-plasmids.plasmid profiles of 80 shigella dysenteriae type 1 (shiga's bacillus) strains, collected between 1974 and 1985 in ethiopia, were examined. strains with the dominant antibiotic-resistance (r-) type--resistance to ampicillin (a), chloramphenicol (c), streptomycin (s), sulphadiazine (su) and tetracycline (t)--showed two distinct plasmid profiles (pp). six plasmid bands were demonstrated in "ethiopian strains" with pp-1a isolated between 1974 and 1982. in mating experiments with these strains, esche ...19902231675
[new aspects of intolerance of the aqueous iodinated x-ray contrast medium, adipiodone--fatal interaction of adipiodone-ultrabil with the immunomodulators corynebacterium parvum (cp) and shigella dysenteriae endotoxin (lps)].the authors assume a new type of interaction of the aqueous x-ray contrast substance type adipiodone (ultrabil 50% spofa) with immunomodulators of corynebacterium parvum (cp) and the endotoxin of shigella dysenteriae (lps). the fatal type of interaction developed regularly as a result of the concurrent i.v. administration of the x-ray contrast substance adipiodone with the endotoxin of shigella dys. (lps) to laboratory animals (mice) which had five days previously a single dose of corynebacteriu ...19902237238
methotrexate as an adjunct in the treatment of persistent mild cardiac allograft rejection.because methotrexate arrests inflammation in autoimmune disease, we studied its efficacy in persistent low-grade cardiac allograft rejection. seventeen patients aged 39.5 +/- 0.9 years (mean +/- se) had persistent rejection despite previous therapy with high dose corticosteroids. maintenance immunosuppression consisted of prednisone, azathioprine, and cyclosporine. the rejection episode treated with methotrexate occurred 180 +/- 55.4 days posttransplantation. patients had incurred 2.7 +/- 0.3 pr ...20072238053
methotrexate as an adjunct in the treatment of persistent mild cardiac allograft rejection.because methotrexate arrests inflammation in autoimmune disease, we studied its efficacy in persistent low-grade cardiac allograft rejection. seventeen patients aged 39.5 +/- 0.9 years (mean +/- se) had persistent rejection despite previous therapy with high dose corticosteroids. maintenance immunosuppression consisted of prednisone, azathioprine, and cyclosporine. the rejection episode treated with methotrexate occurred 180 +/- 55.4 days posttransplantation. patients had incurred 2.7 +/- 0.3 pr ...20072238053
mechanism of the vasodilator action of pinacidil.the mechanism of the vasodilator action of pinacidil has been studied in rat mesenteric small arteries. the results show, first, that the use of flux studies to make measurements of ion permeability requires knowledge of the membrane potential, especially as regards k+ permeability. second, the results confirm that the vasodilator effect of pinacidil is due to an increase in k+ permeability. lastly, the results suggest that the k+ channels involved are sensitive to glibenclamide.19902242449
correlation between beta 2-microglobulin and soluble interleukin-2 receptor levels in plasma of individuals living in a malarial endemic region.beta 2-microglobulin (beta 2m) levels were related to the expected immunoprotection in 81 individuals living in a malarial mesoendemic area near bobo-dioulasso (burkina faso), who were longitudinally followed. soluble interleukin-2 receptor (sil-2r) levels were positively correlated to those of beta 2m (r = 0.44; n = 237; p less than 0.001). this suggests that most of the beta 2m could have originated from activated t and b cell membrane turnover. in our study, both beta 2m and sil-2r were inver ...19902242964
[surgical scoliosis therapy using vds and harrington instrumentation (ventral derotation spondylodesis)].the vds-method in accordance to zielke and the harrington-distraction-spondylodesis are proved as methods for correction of scoliosis lasting for decades, which late results are world-wide analysed and published. the both diametrical corrective principles and the fundamental different operative techniques settle their different indications, which doubtless depend on the experience of the surgeon. both methods complete each other in operative spectrum of the spinal surgeon. a careful study of the ...20082248610
[bacillary dysentery--1988]. 19902251385
residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments.residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ...20072258513
residual effectiveness of insecticides for control of german cockroaches (dictyoptera: blattellidae) in food-handling establishments.residual effectiveness of insecticides was compared for two treatment methods at various sites within two commercial kitchens. for all sites and insecticides, german cockroach, blattella germanica (l.), mortality was greatest for treatments of cracks and crevices than for treatments of exposed surfaces. mortality at the dishwashing site was lower than at all other sites. we attributed reduced mortality at the dishwashing site to a higher vapor pressure deficit. mortality and the amount of insect ...20072258513
the differential efficacy of antihypertensive agents in the elderly.a number of controlled trials have shown that antihypertensive therapy is beneficial to the elderly (greater than or equal to 60 years of age). even so, there may be deleterious age-related effects associated with such therapy. because of physiological and pathophysiological problems peculiar to the elderly (many of which remain to be defined precisely), both benefits and untoward effects of antihypertensive drugs differ, sometimes within classes of agents themselves. none of the traditional age ...20082258887
[foreign bodies of the digestive tract]. 20082259119
[bacteriology and epidemiology of shigellas isolated at the army hospital in djibouti].in a space of 26 months 108 shigellas were isolated from 104 patients with sporadic and benign diseases. 90% of the subjects were young french. both s. dysenteriae and s. boydii make up 21% of the strains studied. 13% of the germs are multiresistance. multiresistance can enhance development of serious sickness if a virulent strain comes in.19902263188
structural aspects of pathology in alzheimer's disease.the characteristic lesions of alzheimer's disease, neurofibrillary tangles and neuritic plaques, are the sites of accumulation of abnormal fibrillar material. the structure of the paired helical filament from tangles has been analysed by electron microscopy and biochemical studies have shown that it contains microtubule associated protein tau as a component. fibrils of beta-amyloid in the neuritic plaque arise by polymerization of a small proteolytic fragment of a much larger precursor protein. ...20082268679
survey of enteropathogenic agents in children with and without diarrhoea in ghana.a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ...19902270006
critical care medicine: activities, advances, and implications for infection control. 20082271151
independent eye movements in the turtle.in order to evaluate the normal eye movements of the turtle, pseudemys scripta elegans, the positions of each eye were recorded simultaneously using two search-coil contact lenses. optokinetic nystagmus (okn) was strikingly unyoked in this animal such that one eye's slow-phase velocity was substantially independent of that of the other eye. on the other hand, the fast-phase motions of both eyes occurred more or less in synchrony. an eye's slow-phase gain is primarily dependent on the direction a ...19942271458
fatty streak development in the vicinity of aortic coarctations in hypercholesterolemic rabbits.the influence of altered local hemodynamics on fatty streak development in rabbits fed high cholesterol diets was investigated. an aortic coarctation was created in the abdominal aorta of nine rabbits by placing a partially constricting gold or silver band (1.7 mm x 10 mm) around the aorta between the renal arteries and aortic bifurcation. controls were 20 rabbits; seven sham operated and 13 unoperated rabbits. the abdominal aorta 1-2 cm proximal to the coarctation showed lipid deposition involv ...19902271758
haematological investigation in neonates. 19902271791
the physiological and psychological effects of the expression and inhibition of emotion.previous work has indicated that humor enhances immunity, but the immunological effects of overt crying have not been studied. thirty-nine women viewed sad and humorous videotapes and either inhibited or expressed overt expressions of laughter and weeping; these were observed and timed. moods and immunity (s-iga) were repeatedly measured. the humorous stimulus resulted in improved immunity, regardless of the overt laughter expressed; overt crying was immunosuppressive, whereas the inhibition of ...19902271804
bovine spongiform encephalopathy (bse): menace or mirage? 19902271904
[role of protons and bicarbonate in adaptative gastric cytoprotection].the unknown mechanism of adaptative gastric cytoprotection (agc) induced by 20% ethanol and subsequent injury with 70% ethanol was studied in wistar rats. pretreatment with indomethacin or hgii2 did not prevent the agc, there suggesting that neither endogenous pgs nor gastric mucus take part in its mechanism. on the other hand, ranitidine pretreatment blocked and even aggravated the damage induced by ethanol-ethanol. in contrast, the latter phenomenon was reverted by 20% acidified ethanol. it is ...19902275313
in vitro diffusion of lidocaine across endotracheal tube cuffs.smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ...19902275911
in vitro diffusion of lidocaine across endotracheal tube cuffs.smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ...19902275911
antenatal diagnosis of parenti-fraccaro type achondrogenesis. 20082276781
polysplenia in a fetus with bradycardia from 26 to 36 weeks' gestation, complex cardiac malformations, and heart block.polysplenia is one of several splenic anomalies. frequently, as in the case presented here, significant cardiac anomalies coexist. these cardiac malformations determine the clinical course. in this case, early death was expected and occurred. this patient is different from others described in the literature in that persistent fetal bradycardia was noted at 26 weeks' gestation.20082276938
[supernumerary teeth in the deciduous dentition].the authors presented sovrannumeraries (s) teeth in primary dentition, and the pedodontic, surgical and orthodontic problems. semeiotics signs, and x ray suggested, are described. all the types of s. teeth are showed: supplementaries, conoides, tuberculates and infundibuliformes. for every type characteristics, problems and surgical timing are described. tuberculates and infundibuliformes teeth are the most dangerous for the permanent teeth interrupted: eruption delay, dilacerations, impactions, ...19912288972
enzyme-linked immunosorbent assay for shiga toxin and shiga-like toxin ii using p1 glycoprotein from hydatid cysts.shiga toxin from shigella dysenteriae type 1 strains and shiga-like toxins (slt) i and ii from escherichia coli bind to terminal alpha-d-galp-(1----4)-d-galp containing glycolipids. hydatid cyst fluid isolated from sheep infected with echinococcus granulosus contains a glycoprotein (p1gp) with a terminal alpha-d-galp-(1----4)-d-galp disaccharide. preparations of p1gp were shown to interact directly with shiga toxin and to inhibit the binding and cytotoxicity of shiga toxin to hela cells. a sandw ...19902295845
death in shigellosis: incidence and risk factors in hospitalized patients.the total number of admissions and deaths of patients with shigellosis were ascertained at the dhaka treatment centre of the international centre for diarrhoeal disease research, bangladesh, 1974-1988, and the characteristics of 67 patients who died were compared with those of 134 discharged alive. of 9780 shigella-infected inpatients, 889 (9.1%) died; 32.3% of deaths occurred in children less than 1 year of age. fatality rates were highest (10.3%) in shigella sonnei-infected patients and lowest ...19902313128
evaluation of children with possible appendicitis using technetium 99m leukocyte scan.we evaluated the use of technetium 99m albumin colloid white blood cell (tac-wbc) scan in 33 children with possible appendicitis. ten children had appendicitis, four of whom had perforated their appendix at the time of surgery. children with appendicitis differed from children without appendicitis in the incidence of right lower quadrant rebound tenderness (60% vs 17.4%), and white blood cell count (x10(3)/mm3) (14.0 + 1.9 vs 10.7 +/- 1.0). tac-wbc scans were interpreted as either positive, nega ...20082330248
[hepato-renal polycystic disease and liver hydatidosis (a rare association)].a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ...19902346686
[study of the blink reflex in spinocerebellar degeneration].blink reflex (br) was composed of r1 and r2. r1 is considered to indicate the brainstem function. the purpose of this study was to evaluate the brainstem function of spinocerebellar degeneration (scd) by the r1 latency (r1). br was evoked by the percutaneous stimulation of supraorbital nerve. and r1 was measured by the action potential of orbucularis oculi muscle. direct response was recorded by the percutaneous stimulation of facial nerve to determine the terminal latency of facial nerve (d). b ...20082364628
kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate.the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ...19902365688
[retinal artery occlusion: etiology and risk factors apropos of 151 cases].a retrospective study covering 151 patients was conducted in order to determine the incidence of risk factors (arterial hypertension, diabetes mellitus, smoking, hyperlipidemia, and cardiovascular disorders) in cases of retinal artery occlusion. occlusion of the central artery was found in 43% of the patients, occlusion of one or several branch arteries in 53%, and cilioretinal artery occlusion in 4%. a monofactorial study of risk factors produced a statistically significant value only for arter ...19762366474
role of bacterial and host factors in the pathogenesis of shigella septicemia.to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ...19902373088
tissue integration of a12o3-ceramic dental implants (frialit)--a case report.the tissue intraosseous integration of a12o3-ceramic dental implants (frialit) is studied in a patient case, where an implant-fracture made it necessary to remove the implant from a patient's jaw. the surgical removing of the apical part of the implant with the use of a trepan bur made it possible to examine and visualize histologically and microradiographically the tissue adjacent to the implant. the results of both the histological and microradiographical studies showed that a good healing and ...20072374995
a method for detecting shiga toxin and shiga-like toxin-i in pure and mixed culture.shiga toxin and shiga-like toxins (slts, syn. verotoxins) are currently detected by tissue culture assays that are expensive, time-consuming and require specialised facilities and experienced personnel. we have developed a rapid method to detect shiga toxin and slt-i (verotoxin 1) based on their binding to globotriosyl ceramide (gb3). bound toxin was then detected by an enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies. the direct detection of cytotoxins from pure culture plat ...19902391709
role of ceramide as a lipid mediator of 1 alpha,25-dihydroxyvitamin d3-induced hl-60 cell differentiation.the treatment of hl-60 myelocytic leukemia cells with 1 alpha,25-dihydroxyvitamin d3 (1,25-(oh)2d3) resulted in the activation of a neutral sphingomyelinase and in sphingomyelin turnover (okazaki, t., bell, r., and hannun, y. (1989) j. biol. chem. 264, 19076-19080). in this paper, the effects of 1,25-(oh)2d3 on the product of sphingomyelin hydrolysis, ceramide, and the possible function of ceramide as a lipid mediator of the effects of 1,25-(oh)2d3 on hl-60 cell differentiation were investigated ...20072394750
functional analysis of the shiga toxin and shiga-like toxin type ii variant binding subunits by using site-directed mutagenesis.the b subunit of shiga toxin and the shiga-like toxins (slts) mediates receptor binding, cytotoxic specificity, and extracellular localization of the holotoxin. while the functional receptor for shiga toxin, slt type i (slt-i), and slt-ii is the glycolipid designated gb3, slt-ii variant (slt-iiv) may use a different glycolipid receptor. to identify the domains responsible for receptor binding, localization in escherichia coli, and recognition by neutralizing monoclonal antibodies, oligonucleotid ...19902404947
the colon in shigellosis: serial colonoscopic appearances in shigella dysenteriae i.twenty-five patients (16 males and 9 females; mean age 24.7 +/- 8.4 years) with acute colitis caused by shigella dysenteriae i were studied, a total of 115 colonoscopic examinations being performed. twenty-two patients had weekly colonoscopic examinations until the last procedure revealed normal colonic mucosa. six abnormal colonoscopic appearances were documented, namely mucosal edema, ulcers, friability, punctate spots, erythematous areas and luminal exudate. mucosal edema was the dominant fin ...19902407526
axonally transported shigella cytotoxin is neuronotoxic.shigella dysenteriae strains produce an exotoxin (sdt) which inhibits protein synthesis in susceptible cells and is neurotoxic in some species. intraneural microinjection of highly purified sdt into the cervical vagus nerves of rats, mice, guinea pigs and rabbits produced cytopathic changes within 24 hours in vagal sensory but not motor neurons. these changes consisted of an initial loss of nissl substance followed by progressive cell degeneration and resulted in permanent neuronal loss. indirec ...19852411880
'o'-antigens are essential virulence factors of shigella sonnei and shigella dysenteriae 1.a novel transposon mutagenesis system has been developed for shigella. we have used it to isolate specific mutants of shigella sonnei and shigella dysenteriae 1 that fail to produce lipopolysaccharide 'o' side chains. the virulence of the mutants was evaluated in the sereny test and in a hela cell invasion assay. all ho'-minus mutants failed to provoke a positive sereny reaction but retained the ability to invade hela cells. this demonstrates that 'o' side chains are virulence factors of s. sonn ...19852413650
inactivation by polymyxin b of the endotoxin-mediated interferon production in the rabbit.polymyxin b (pb) completely or at least significantly inhibited the capacity of shigella dysenteriae 1 cells and the lipopolysaccharide (lps) and lipid a (la) subunits of several bacterial endotoxins to induce interferon (ifn) in rabbits. animals injected with lps inactivated by pb to the point of not inducing detectable ifn levels did not develop hyporesponsiveness to secondary ifn induction by a homologous inducer. it was concluded that pb inhibits the ifn-inducing capacity of endotoxin and it ...19852413651
[vital staining of the conjunctiva for prevention of epithelial implantation cysts].selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ...19852415747
growth factors, angiogenesis and metastasis. 19862424027
prothrombin requires two sequential metal-dependent conformational transitions to bind phospholipid. conformation-specific antibodies directed against the phospholipid-binding site on prothrombin.prothrombin is a gamma-carboxyglutamic acid-containing protein that binds to phospholipid vesicles in the presence of calcium ions after undergoing a metal ion-induced conformational transition. to integrate recent data into a scheme that is compatible with our knowledge of prothrombin-metal interaction, we have proposed a new model of prothrombin structure. in this model prothrombin undergoes two metal-dependent conformational transitions: pt----pt'----pt*. the first transition is not cation-sp ...20082429962
immunofluorescence detection of cryptosporidium oocysts in fecal smears.an indirect fluorescent antibody (ifa) procedure was developed for the detection of cryptosporidium sp. oocysts in human, nonhuman primate, and bovine fecal smears. the procedure, which takes about 90 min to perform, involves the use of a rabbit antiserum against cryptosporidium oocysts isolated from dairy cattle. cross-specificity testing of the ifa method revealed no reactivity with yeasts, various amoebae, giardia lamblia, chilomastix sp., or blastocystis sp. and only very weak cross-reactivi ...19862429982
sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells.changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain c3 were exposed to the fungal toxins, sporidesmin or gliotoxin. both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. other changes included cytoplasmic vacuolation and blocked entry into s-phase of the cell cycle. under these conditions [3h]thymidine incorporation into the cells was also diminished but ...19862432076
component of whole cell ca current due to electrogenic na-ca-exchange in cardiac myocytes.a carrier mechanism that mediates an exchange of na+ for ca2+ across the cell membrane is believed to be an important regulator of intracellular ca2+ and therefore the contractile strength of the heart. considerable evidence indicates that there is an unequal transfer of electrical charge during this exchange which implies that the carrier may contribute substantially to the net flow of ionic current across the sarcolemmal membrane. to detect such a component of current, nondialyzed single frog ...20062435177
monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains.monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ...19872437036
[photocoagulation with krypton laser of subretinal vessels in macular degeneration related to age. long-term results (apropos of 100 cases)]. 20082440603
a mucin containing the x, y, and h type 2 carbohydrate determinants is shed by carcinoma cells.monoclonal antibody br 15-6a directed to the y carbohydrate determinant (fuc alpha 1----2gal beta 1----4glcnac(3----1fuc) beta 1----3gal beta 1----4glc beta 1----1cer) reacted with the cell surface and conditioned media of colorectal and breast carcinoma cell lines. double determinant immunoassays using br 15-6a as detector antibody showed that the y determinant is part of a high molecular weight mucin that coexpressed other carbohydrate antigens based on a type 2 chain (x, h type 2). type 1 cha ...20082442094
[structure of the o-specific polysaccharide chain of shigella dysenteriae type 7 lipopolysaccharide].the structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide has been established mainly by 13c nmr analysis of the intact and modified (acetylated and de-o-acetylated) polymers, as well as of products of its solvolysis with anhydrous hydrogen fluoride. the polysaccharide contains two unusual sugar derivatives. n-acetyl-d-galactosaminuronamide and 4-(n-acetylglycyl)amido-4,6-dideoxy-d-glucose (galnacan and qui4n----glyac, respectively) and is built u ...19872445353
the effects of the protein synthesis inhibitor anisomycin on the febrile responses to intracerebroventricular injections of bacterial pyrogen, arachidonic acid and prostaglandin e2.1. anisomycin (15 mg/kg) was administered s.c. to cats at ambient temperatures of 5 degrees c, 20 degrees c and 38 degrees c. it produced biphasic effects on body temperature at 5 degrees c and 20 degrees c, an initial fall in temperature followed by a rise in body temperature, and a rise in body temperature of long latency at 38 degrees c. 2. anisomycin (15 mg/kg) attenuated the hyperthermic responses to centrally injected pge2 (1 microgram) at all ambient temperatures studied and also complete ...19872446153
comparative effects of adriamycin and dna-non-binding analogues on dna, rna, and protein synthesis in vitro.drug-dna binding is claimed to be the basis by which the antitumor antibiotic adriamycin (doxorubicin) inhibits dna and rna synthesis in vitro. however, in preliminary studies the dna-non-binding adriamycin analogue n-trifluoroacetyladriamycin-14-valerate (ad 32) showed somewhat greater inhibition of dna and rna synthesis than adriamycin under identical conditions. the kinetics of macromolecule synthesis inhibition induced by adriamycin and ad 32, and the two principal dna-non-binding metabolite ...19872446792
effects of substance p, neurokinin a and calcitonin gene-related peptide in human skin and their involvement in sensory nerve-mediated responses.the effects evoked by intradermal injections of substance p (sp), neurokinin a (nka) or calcitonin gene-related peptide (cgrp) were studied in 51 non-atopic subjects. sp and nka produced flare and weal, and cgrp produced an indurated erythema. the reactions to sp were strong, the flare being maximal 3-5 min after injection and the weal after 10-15 min. nka evoked a much weaker flare and a slightly weaker weal than did sp. cgrp produced a prominent long-lasting, indurated erythema with pseudopod ...19872446892
structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern.anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ...19872447883
the stability of o-antigen plasmid is determined by a chromosomal region of shigella dysenteriae 1.it is well established that plasmids are involved in the expression of lipopolysaccharide in certain species of shigella. in shigella sonnei, both the biosynthesis of oligosaccharide side chains (o antigen), and cell invasiveness are controlled exclusively by a 120 megadalton (mda) plasmid. in shigella dysenteriae 1, a 10 kilobase (kb) plasmid is required for o-antigen production. shigella dysenteriae 1 strains devoid of this plasmid lose the ability to synthesize o antigen. interestingly, this ...19882454151
epitope mapping of hla-b27 and hla-b7 antigens by using intradomain recombinants.to study the hla-b7 and hla-b27 antigenic determinants, hybrid genes between these two alleles were constructed by in vivo recombination in escherichia coli. after transfection of these genes into p815 (high transfection efficiency recipient) murine cells, the bindings of bw6, hla-b7, and hla-b27 allele-specific mab were studied, as well as that of human anti-hla-b7 and anti-hla-b27 monospecific alloantisera. most of the hla-b7 antigenic determinants were assigned to the first external domain of ...19882459214
ifn-treated neuroblastoma cell lines remain resistant to t cell-mediated allo-killing, and susceptible to non-mhc-restricted cytotoxicity.neuroblastoma cell lines can have very low mhc ag expression. the cell lines are insensitive to allo-killing by primed ctl, but are sensitive to non-mhc-restricted cytotoxicity. ifn-gamma increased class i expression, but the cells remained insensitive to ctl. susceptibility to nonrestricted effectors was preserved. class i+ glioma cell lines behaved similarly. the ctl resistance was localized to the recognition phase. neuroblastoma lines did not form conjugates with primed t cells, but were lys ...20082459235
somatic antigens of shigella: structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide.4-(n-acetylglycyl)amino-4,6-dideoxy-d-glucose has been identified as a component of the shigella dysenteriae type 7 o-specific polysaccharide, in addition to the previously reported 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galacturonic acid. on the basis of selective cleavage with anhydrous hydrogen fluoride and analysis by 1h- and 13c-n.m.r. spectroscopy and f.a.b.-mass spectrometry, it was concluded that the tetrasaccharide repeating-unit of the polysaccharide has the following ...19882463086
purification and characterization of a phage-encoded cytotoxin from an escherichia coli o111 strain associated with hemolytic-uremic syndrome.cytotoxin production by escherichia coli o111:h-strain hus-2 (hamburg) is associated with a temperate toxin-converting bacteriophage (tcp-111). e. coli laboratory strain c600 transduced and subsequently lysed by the phage produced and liberated large amounts of cytotoxin (ct111) which was purified by sequential chromatography. when compared with published procedures for toxin release from viable cells, lysis of the c600 culture by the phage was most effective. by sds-page ct111 as shiga toxin fr ...19882464885
enteropathogens isolated from children with gastro-enteritis at ga-rankuwa hospital, south africa.a prospective 3-year study for enteric pathogens associated with diarrhoea in children was undertaken at ga-rankuwa hospital, south africa. rotavirus was the most commonly isolated pathogen (24%), showing a marked seasonal distribution, with an autumn peak in activity. enterotoxigenic and enteropathogenic escherichia coli were frequently identified, and salmonella, shigella and campylobacter were also isolated. cryptosporidium and giardia lamblia were the most frequently seen parasites. rotaviru ...19882467616
cloning of the rfb gene region of shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines.recent studies have shown that determinants for the production of o antigen lipopolysaccharide in shigella dysenteriae 1 are distributed over two distinct genetic elements, the chromosome and a 9 kb plasmid designated phw400. in this communication, we describe the cloning of all determinants necessary for s. dysenteriae 1 o antigen production in e. coli k-12 and their combination in a single plasmid. an rp4::minimu r-prime plasmid, r-prime 40, containing the his-rfb (histidine biosynthesis-lipop ...19862469931
genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: 9 kb plasmid of s. dysenteriae 1 determines addition of a galactose residue to the lipopolysaccharide core.production of the somatic antigen, o-specific polysaccharide of shigella dysenteriae 1 is determined by the chromosomal rfb gene cluster and the rfp gene located on the 9 kb plasmid phw400 carried by this organism. when transferred to escherichia coli k-12, which produces lipopolysaccharide consisting only of core oligosaccharide linked to lipid a, rfp gene-containing plasmids caused modification of the core oligosaccharide leading to the appearance of core molecules with new electrophoretic mob ...19862469932
genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: structure and functions of the rfb gene cluster.the genetic organization and functions of the shigella dysenteriae 1 rfb gene cluster, which specifies the somatic o antigen in this organism, have been studied in escherichia coli k-12 by insertion and deletion mutagenesis of pss9, a pbr322 hybrid containing the shigella rfb genes. on the basis of the sensitivity/resistance to rough-specific bacteriophage t3 of e. coli k-12 derivatives containing mutant pss9 plasmids, of the banding patterns and immunoreactivity of lps isolated from such deriva ...19862469933
production and characterisation of monoclonal antibodies to verotoxins 1 and 2 from escherichia coli of serotype o 157:h7.fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (mabs) to purified verotoxins 1 and 2 (vt1, vt2) of escherichia coli of serotype o157:h7. of these mabs, eight were obtained by immunisation of balb/c mice with purified vt1, and six were obtained from balb/c mice immunised with purified vt2. with the exception of mab 1c5, with a heavy chain of igg2b class, antibodies produced from mice immunised with heat-treated toxin were of igm class. mabs produced from mice immu ...19892479749
effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins.the interferon (ifn)-inducing activity of detoxified lipopolysaccharide (lps) was tested in rabbits treated with lps preparation derived from escherichia coli, salmonella typhi, salmonella enteritidis and shigella dysenteriae serovar 1. of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the ifn-inducing potential of lps. in contrast, acet ...19892484274
studies on shiga-like toxin produced by enterohemorrhagic escherichia coli: purification and characterization of the toxin and development of methods for identifying the toxin.a simple purification method using deae cellulose column chromatography and immunoaffinity column chromatography was developed for purifying shiga-like toxin produced by escherichia coli o157:h7. about 0.75 mg of purified toxin was obtained from 5 liters of culture (62% recovery). the purified toxin was demonstrated to be immunologically, biologically and structurally indistinguishable from shiga toxin. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga-lik ...19892485395
the complexion and complexity of the nursing profession. 19952486684
morbidity pattern in aged persons attending rural health centre in haryana. 19972486987
[imported myiasis: analysis of 5 cases].five cases of furuncular myiasis imported by spanish travelers from tropical countries are reported. after reviewing the cycle of the infecting organism and the characteristics of the human disease, a table with the clinical and epidemiological data of our patients is presented. the relevance of an adequate clinical and geographical anamnesis in these patients is discussed. finally, the effectiveness of a simple, nonaggressive treatment is reported, which permits the patient to verify by himself ...19892490857
nonrandom rearrangement of chromosome 14 at band q32.33 in human lymphoid malignancies with mature b-cell phenotype.chromosomes were studied in 61 patients with differentiated b-cell malignancies including 21 with non-hodgkin's lymphoma (nhl), three with hairy cell leukemia (hcl), eight with waldenström's macroglobulinemia (wm), and 29 with plasma cell disorder. chromosomally abnormal clones were identified in 35 of 61 patients studied: all with nhl, all with hcl, three of eight with wm, and eight of 29 with plasma cell disorder. the most recurrent chromosomal abnormality, observed in 26 of the 35 patients wh ...19892492904
four new provisional serovars of shigella.four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ...19892501349
hemagglutinating properties of shigella dysenteriae type 1 and other shigella species.strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ...19892503449
tongue flaps in repair of large palatal defects.anteriorly based dorsal tongue flaps were used in 19 cleft patients for closing large palatal defects. the procedure was successful in 17 patients. one patient had partial marginal necrosis after division of the pedicle, while another had complete necrosis of the distal part of the flap. our results support the view that for success of the procedure, adequate length, width and thickness of the flap are important, while the height of the palate also plays a role.20082509583
[increased efficiency in the disinfection of sewage by lactic acid bacteria].the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ...19892514340
[in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics].in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ...19892518646
[evaluation of results of school health examination in the murcia region. 1986/87].in the present study, we have carried out an evaluation of school examinations in 47,943 children in the past course. we have compared our results with those from similar previous studies in other parts of spain. our findings were comparable in visual acuity and scoliosis, and not so much about cryptorchidism and phimosis. we think that examinations are necessary, but it is needed to increase the quality and the effectiveness, to get closer to the knowledge of usefulness and to the correct evalu ...20082518932
[cardiac tamponade as the first manifestation of cancer].we report 3 patients having cardiac tamponade as the initial presentation of cancer. at the onset of symptoms all three patients were incorrectly diagnosed. pulsus paradoxus and cardiomegaly on the chest x-ray were the main clinical features. two dimensional echocardiography was vital revealing massive pericardial effusion with diastolic right atrial and ventricular collapse. all patients had bloody pericardial fluid removed and the histopathology of the metastatic pericardial tissue showed lung ...20082519419
identification of shiga-like toxin in escherichia coli strains, etiological agents of diarrheal disease.shiga-like toxin presence, in 20 e. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees c, in the presence of chloroform and by i.v. inoculation in mice. in 4 out of 20 strains, shiga-like toxin in high titres was identified. most of the strains presented an inconstant and variable production of shiga-like toxin in comparison with shigella dysenteriae type 1 (shiga) reference strain. the authors also confirm the existence of shiga-like toxin und ...19892520669
[the dependence of hemoglobin and iron reserve indices on habitat conditions (sampling research in osh province, the kirghiz ssr)].sampling investigation of female population (896 subjects) was conducted in five regions with opposite environmental conditions. a relatively low incidence of iron deficiency anemia and high incidence of exhausted iron reserves in the women living in the high mountains indicates the participation of the adaptive-compensatory mechanisms in the development of iron-deficiency. a high rate of iron deficiency incidence in the regions of intensive tobacco growing and association of iron-deficiency ane ...19892533125
effects of the nmda receptor antagonist mk-801 against amygdaloid-kindled seizures in the rat.the effects of acute and subchronic administration of the noncompetitive nmda receptor antagonist mk-801 on the duration and severity of amygdaloid-kindled seizures in rats were determined to evaluate its anticonvulsant activity. both the highest acute (1000 micrograms/kg) and subchronic regimens (2 x 300 micrograms/kg, 7 days) produced significant reductions in the seizure stage and afterdischarge duration. although ataxia may have affected the seizure stage, mk-801 attenuated the primary after ...19892536332
multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription.transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ...19892542891
dual function of a nuclear factor i binding site in mmtv transcription regulation.using linker-scanning mutagenesis we had previously identified four elements within the mmtv ltr which are necessary for transcriptional stimulation by glucocorticoid hormones. two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. the third element contained a nf-i binding site, and the fourth the tata box. here we show that mutations that abolish in vitro binding of nf-i had a negative effect also on the basal activity of the mmtv promoter of ltr-containing pl ...19892542892
[a germ cell tumor of an ectopic testis in polyorchidism]. 20082547104
influence of diethyldithiocarbamate on nickel-induced hepatic metallothionein in rats.the influence of sodium diethyldithiocarbamate (ddc) on 63nickel chloride-induced metallothionein (mt) and alterations in essential metal levels in the liver of rats was investigated. the induction of mt, zn and cu levels of the hepatic cytosolic "heat stable fraction" (hsf) by ddc increased with time up to 24 hr. although mt, zn and cu were significantly greater at 17 hr than those at 6.5 hr after 63ni administration, the ni level decreased. the treatment with ddc at 6 hr, but not at 10 min, pr ...19892548306
phosphate regulon in members of the family enterobacteriaceae: comparison of the phob-phor operons of escherichia coli, shigella dysenteriae, and klebsiella pneumoniae.the structure and function of the phob and phor genes of shigella dysenteriae strains and klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. complementation tests among the genes of escherichia coli, s. dysenteriae strains, and k. pneumoniae for production of alkaline phosphatase indicate that s. dysenteriae serotype 2 and serotype 3 strains and k. pneumoniae are phoa+ phob+ phor+ but s. dysenteriae sh and serotype 1 strains are phoa phob+ phor. nucl ...19892556368
characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli.the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ...19892556396
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