PMID(sorted ascending)
[can vision defects among artists influence their art?]. 20061895830
endocytosis and intracellular transport of the glycolipid-binding ligand shiga toxin in polarized mdck cells.the glycolipid-binding cytotoxin produced by shigella dysenteriae 1, shiga toxin, binds to mdck cells (strain 1) only after treatment with short-chain fatty acids like butyric acid or with the tumor promoter 12-o-tetradecanoylphorbol 13-acetate. the induced binding sites were found to be functional with respect to endocytosis and translocation of toxin to the cytosol. glycolipids that bind shiga toxin appeared at both the apical and the basolateral surface of polarized mdck cells grown on filter ...19911901867
bacteriological and protozoal study on domestic water supply in karachi.four hundred and fifty eight water samples collected from domestic tanks and taps and community taps were analysed for bacterial contamination. faecal pollution was more in domestic tanks and taps in mehmoodabad, korangi and least in water collected from community taps of liaquatabad, korangi and mehmoodabad. bacteria isolated were mostly e. coli, enterobacter sp, klebsiella sp. and a. faecalis, while other organisms (except pseudomonas) were found in lower numbers. salmonella para typhi and par ...19911902529
differential effects of gonadotrophin-releasing hormone agonists administered as desensitizing or flare protocols on hormonal function in the luteal phase of hyperstimulated cycles.the incorporation of gonadotrophin-releasing hormone agonist (gnrha) in in-vitro fertilization (ivf) stimulation protocols has led to doubt about the quality of the subsequent luteal phase. the effects of two gnrha stimulation protocols on luteal phase concentrations of oestradiol (e2), progesterone (p), luteinizing hormone (lh) and follicle stimulating hormone (fsh) were compared with the standard clomiphene stimulation regimen. subjects receiving clomiphene with human menopausal gonadotrophin ...19911905309
new trends in the use of al(oh)3-conjugated endotoxins and their subunits from the s- and r-forms of shigella dysenteriae serovar 1 for model vaccination purposes.the lipopolysaccharides, lipopolysaccharide-protein complexes and their lipids a, isolated from shigella dysenteriae 1, exhibited lethal toxicity (ld50 300-400 micrograms per mouse), pyrogenicity (0.01-1.0 microgram), activity in the limulus test (10(-3)-10(-12) mg ml-1) and produced a positive local shwartzman reaction. it was found that these substances bound to al(oh)3 were capable of successfully protecting mice from challenge with both homologous and heterologous infections (up to 90% of mi ...19911905447
antimicrobial activity of microgard against food spoilage and pathogenic microorganisms.microgard, a commercially available fermented milk product containing antimicrobial metabolites, was a potent inhibitor for gram-negative bacteria such as pseudomonas, salmonella, and yersinia when 1% concentration was incorporated into agar media. gram-positive bacillus cereus, staphylococcus aureus, and listeria monocytogenes were insensitive to microgard. kluyveromyces marxianus, an unidentified black yeast, and penicillium expansum were partially suppressed, whereas aspergillus niger and a y ...19911906486
screening of children for enteric bacterial pathogens in the outborn neonatal ward in lagos, nigeria.babies, on admission into a neonatal ward at the lagos university teaching hospital, had their rectal swab specimens examined bacteriologically and screened for enteric bacterial pathogens over a one-year-period at two-week intervals. it was found that on the average there were 3 (9.68%) enteric bacterial pathogens out of an average of 31 admissions at each screening period. the enteric bacterial pathogens isolated included: non-typhoid salmonellae, which accounted for 55 (80.88%) isolates out o ...19911915794
reemergence of shigella dysenteriae type 2 in sicily: an epidemiological evaluation.three strains of shigella dysenteriae type 2 were isolated from a small household outbreak which occurred in palermo, sicily, during summer 1990. two isolates were recovered from hospitalized patients and one from an asymptomatic carrier. the infection could not be associated with travel to foreign countries or contact with travellers returned from abroad. since 1953 s. dysenteriae has been never isolated in southern italy. the isolates from dysentery cases were susceptible to antibiotics and ca ...19911921742
shigellosis. shigella dysenteriae type 1. 19911931580
inner city psychiatry. 20071932958
myopic excimer laser keratectomy. a preliminary report. 20071936430
preparation, characterization, and immunogenicity of conjugates composed of the o-specific polysaccharide of shigella dysenteriae type 1 (shiga's bacillus) bound to tetanus toxoid.the background for developing conjugate vaccines for shigellosis composed of the o-specific polysaccharide (o-sp) bound to a protein is described elsewhere (c. y. chu, r. schneerson, and j. b. robbins, submitted for publication). briefly, there is direct evidence for type (lipopolysaccharide [lps])-specific protection after infection with the wild type or with attenuated strains of shigellae. prospective studies of israeli armed forces recruits show a correlation between preexisting serum immuno ...19911937803
increased vitamin e intake restores fish-oil-induced suppressed blastogenesis of mitogen-stimulated t lymphocytes.we sought to determine whether fish-oil supplementation would suppress blastogenesis in vitro of concanavalin a (cona)-stimulated peripheral blood mononuclear cells (pbmcs) and, if so, whether it could be reversed with increased intake of vitamin e. healthy males ate a controlled basal diet providing a total of 40% of energy from fat when fed in conjunction with 15 g/d of either placebo oil (po) or fish-oil concentrate (foc) fortified with 15 mg alpha-tocopherol/d for three periods. the subjects ...20071951163
age- and sex-specific cycles in united states suicides, 1973 to us suicides peak on the first day of each week, in the first week of each month, and in the late spring of each year. although these cycles are assumed to characterize all us suicides, analyses by age and sex show that the cycles occur only in a few subpopulations. day-of-the-week effects are found almost exclusively in middle-aged suicides; day-of-the-month effects are found almost exclusively in elderly male suicides; and month-of-the-year effects asre found almost exclusively in teenage ...20071951813
esophageal function and dysfunction. 20071951923
[maternal morbidity and mortality in extra-genital pathology].the authors analyze the incidence and structure of acute and chronic visceral diseases in pregnant women and the incidence and structure of maternal death because of extragenital diseases. causes of maternal mortality are discussed and approaches to reducing maternal morbidity and mortality in visceral diseases outlined.20071951974
compartmentalization within the nucleus: discovery of a novel subnuclear region.antibodies to a set of structurally related autoantigens (p23-25) bind to a previously uncharacterized, large structural domain in the nucleus of a variety of human cell types. this subnuclear domain is visible by phase contrast alone as a region of decreased density after several different fixation protocols. the morphology of this region changes dramatically during the cell cycle and we have given it the name pika (for polymorphic interphase karyosomal association) based on preliminary evidenc ...19911955462
distribution of insertion sequence is200 in salmonella and shigella.two dna probes for the detection of insertion sequence is200 by either southern blotting or colony hybridization were constructed. one of the probes is a 300 bp ecori-hindiii fragment of is200 cloned onto pbluescript ks(+); the other is a tail-to-tail dimer of the same fragment cloned onto puc19. a survey of the presence of is200 among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of salmonella spp. examined contained one or more copies of insertion se ...19901964174
h(+)-stimulated release of prostaglandin e2 and cyclic adenosine 3',5'-monophosphoric acid and their relationship to bone resorption in neonatal mouse calvaria cultures.the addition of protons to the medium of neonatal mouse calvaria cultures stimulated bone resorption and release of calcium into the medium. in addition, added protons significantly increased the release of prostaglandin e2 (pge2) and cyclic adenosine 3',5'-monophosphoric acid (camp) from the bones. indomethacin significantly inhibited the release of calcium, pge2 and camp from proton-treated cultures. the positive control, parathyroid hormone (pth)-treated cultures, also gave rise to bone resor ...19901964815
production and characterization of monoclonal antibodies with therapeutic potential against shiga toxin.four monoclonal antibodies (t2d1, t3b1, t7b2 and t4c4) were produced and characterized against sh. dysenteriae type 1. these monoclonal antibodies (mcabs) were shown to be directed against the same or adjacent determinants on the a-subunit of the shiga toxin. all the anti-shiga toxin mcabs are igg1 (kappa) antibodies and have the capacity to neutralize the cytotoxic and neurotoxic effects of the shiga toxin: the data strongly suggest that both of the activities, associated with the toxin, are du ...19901966939
pivmecillinam-resistant shigella dysenteriae type 1 infection in bangladesh. 19901972232
inhibition by anti-cd2 monoclonal antibodies of anti-cd3-induced t cell-dependent b cell activation.anti-cd3 mab can activate t cells to help in b cell activation as detected by late events, such as maturation of b cells into ig-secreting cells (igsc), or by early events, such as b cell surface expression of the activation marker cd23. two different anti-cd2 mab each inhibited anti-cd3-induced t cell-dependent b cell activation in a dose-dependent fashion. neither irradiation of the t cells prior to culture nor depletion of cd8+ cells abrogated the inhibitory effects of anti-cd2 mab. despite t ...20071976440
identification of three amino acid residues in the b subunit of shiga toxin and shiga-like toxin type ii that are essential for holotoxin activity.shiga toxin of shigella dysenteriae type i and shiga-like toxins i and ii (slt-i and slt-ii, respectively) of enterohemorrhagic escherichia coli are functionally similar protein cytotoxins. these toxin molecules have a bipartite molecular structure which consists of an enzymatically active a subunit that inhibits protein synthesis in eukaryotic cells and an oligomeric b subunit that binds to globotriaosylceramide glycolipid receptors on eukaryotic cells. regionally directed chemical mutagenesis ...19911991714
production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11.a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ...19911993773
synthetic peptides as the basis for vaccine design. 19912017193
unusual association of a plasmid with nalidixic acid resistance in an epidemic strain of shigella dysenteriae type 1 from asia.the association of a 20-mda plasmid with nalidixic acid resistant (nalr) strains of shigella dysenteriae 1 has been examined. the plasmid, which is readily transferable, does not itself code for nalidixic acid resistance but offers a survival advantage to its host under nalidixic acid stress. the plasmid-containing cultures of s. dynsenteriae 1 produced nalr mutants in vitro at a frequency 1000-fold higher than their plasmidless parent strains, after two exposures to nalidixic acid. using a simi ...19912021898
efficacy of aqueous extract of garlic & allicin in experimental shigellosis in rabbits.the aqueous extract of garlic (allium sativum) and allicin both showed significant in vitro antibacterial activity against isolates of multiple drug-resistant shigella dysenteriae 1, sh. flexneri y, sh. sonnei and enterotoxigenic escherichia coli. the minimum inhibitory concentrations of the aqueous extract and allicin against sh. flexneri y were 5 and 0.4 microliters/ml, respectively. the two agents also showed promising in vivo antibacterial activity against sh. flexneri y when tested in the r ...19912022399
the association of haemagglutination and adhesion with lipopolysaccharide of shigella dysenteriae serotype this study the ability of strains of shigella dysenteriae serotype 1 to agglutinate mammalian erythrocytes is attributed to the polysaccharide fraction of bacterial-cell lipopolysaccharide (lps). lps obtained from a rough, mutant strain of s. dysenteriae serotype 1, lacking the o-antigen polysaccharide side-chain, did not agglutinate erythrocytes, clearly demonstrating a link between o-antigen polysaccharides and haemagglutinating activity (ha). strains of s. dysenteriae serotype 1 adhered we ...19912030501
conversion of oral glucose to lactate in dogs. primary site and relative contribution to blood lactate.we evaluated the relative contribution of oral glucose to arterial lactate and the relative role of the splanchnic bed in converting glucose to lactate in normal healthy dogs. after an oral glucose load (1.2 g/kg) spiked with [u-14c]glucose (16.9 muci/kg; protocol 1, n = 7), arterial blood lactate increased from 0.43 +/- 0.03 mm at basal to a peak of 1.04 +/- 0.07 mm at 45 min and then slowly decreased to 0.47 +/- 0.07 mm at 240 min. arterial blood [14c]lactate peaked at 60 min and then decrease ...19912040389
when is treatment economically justified? 19852041026
four decades of shigellosis in israel: epidemiology of a growing public health problem.despite the improved standard of living in israel, shigellosis remains a common disease. examination of trends in the incidence of shigellosis in israel between 1951 and 1987 showed a period of declining rates followed by an increase in the reported incidence beginning around 1969. this increase was mainly due to shigella sonnei; rates of infection with shigella flexneri remained relatively constant. trends showed marked differences between age groups and between ethnic groups. in comparison, th ...19912041956
insulin and blood pressure regulation.epidemiological evidence suggests that there is a close association between obesity, non-insulin-dependent diabetes (niddm) and hypertension. obesity and niddm are the classical insulin-resistant states. even in the absence of these conditions, essential hypertension is associated with insulin resistance. in view of the acute effects of insulin on renal sodium reabsorption, the sympathetic nervous system, the renin-angiotensin-aldosterone system, the transmembranous cation transport, the cardiov ...20072043223
shigella dysenteriae type 1--guatemala, 1991.on march 14, 1991, physicians at a hospital in guatemala city reported to the institute of nutrition of central america and panama (incap) that a 2-year-old boy living in an orphanage in guatemala city had been hospitalized with dysentery; stool cultures yielded shigella dysenteriae type 1. another child from the orphanage had recently died from dysentery. during march 18-21, two other young children from the orphanage were diagnosed with s. dysenteriae type 1. on march 21, health officials in r ...19912046650
epidemiologic aspects of shigellosis and other causes of dysentery in thailand.nearly 20% of children seen in the outpatient department of children's hospital in bangkok, thailand, for diarrheal disease had bloody diarrhea. shigella species and enteroinvasive escherichia coli--isolated from 13% and 2% of children with diarrhea, respectively--were the most frequent causes of bloody diarrhea. campylobacter species and nontyphoidal salmonella species were also isolated frequently but were much less often associated with bloody diarrhea. shigella species were rarely isolated f ...19912047642
shigellosis in vietnam: seroepidemiologic studies with use of lipopolysaccharide antigens in enzyme immunoassays.the performance of enzyme immunoassays (eias) with use of o-antigen-containing lipopolysaccharides (lpss) extracted with phenol-water from shigella dysenteriae type 1, shigella flexneri serotypes 1a-5b, and shigella sonnei for determination of the serum antibody responses after onset of bacillary dysentery is reviewed. for the purpose of several studies, serum samples from a total of 175 vietnamese and 47 swedish patients, for whom shigella species had been isolated from fecal specimens, were ob ...19912047643
mortality due to shigellosis: community and hospital data.almost all fatal cases of shigellosis occur in developing countries, and data on mortality are generally compiled from three sources: investigations of epidemics caused by shigella dysenteriae type 1, surveillance of endemic diarrheal disease, and reports from hospitals. attack rates during epidemics of dysentery due to infection with s. dysenteriae type 1 have ranged from 1% to 33%, and case-fatality rates have ranged from 1% to 7%. in matlab, a rural district in bangladesh, most diarrhea-relat ...19912047645
shiga toxin: purification, structure, and function.shiga toxin is a potent toxin produced by shigella dysenteriae type 1 strains. the toxin has three biologic activities--cytotoxicity, enterotoxicity, and neurotoxicity--and one known biochemical effect: inhibition of protein synthesis. it consists of two polypeptide chains, an a chain (molecular weight, 32,225) and a b chain (molecular weight, 7,691). these two peptides associate with a stoichiometry of one a and five b subunits to form the holotoxin. the a chain is responsible for the biochemic ...19912047652
digalactosyl-containing glycolipids as cell surface receptors for shiga toxin of shigella dysenteriae 1 and related cytotoxins of escherichia coli.purified glycolipids containing galactose-alpha(1-4)galactose bind with shiga toxin of shigella dysenteriae 1 and shiga-like toxins of escherichia coli with high specificity in both thin-layer chromatograms and glycolipid-coated microtiter plates. these glycolipids appear to be the natural cell surface receptors for this class of bacterial toxins since artificial glycoconjugates block cytotoxicity as well as binding of 125i-labeled toxin. cell lines lacking these glycolipids are refractory to to ...19912047653
shiga toxin: intestinal cell receptors and pathophysiology of enterotoxic effects.shiga toxin is enterotoxic in rabbit small bowel and binds to the microvillus membrane (mvm). the toxin exhibits specificity for glycolipids possessing a terminal gal-alpha 1----4gal disaccharide, including the neutral glycolipid gb3 in mvm. gb3, which is developmentally regulated in the rabbit small bowel, is present in very low concentration until the animals reach day 16 of life. in older animals an increase in gb3 content is paralleled by an increase in the ability of mvm to bind toxin, whic ...19912047654
intestinal manifestations of invasive diarrheas and their from studies of 916 children with diarrhea, including 122 from whom shigellae were isolated, and data on patients affected in an epidemic due to shigella dysenteriae type i were analyzed to determine whether a diagnostic clinical profile of shigellosis could be identified. blood and/or mucus in stool, increased frequency of stool, abdominal pain, rectal tenesmus, and fever were noted more frequently in patients with shigellosis. the diagnostic confirmation of shigellosis depends on the isol ...19912047655
antimicrobial therapy for controlled clinical trials, which were first performed with use of the sulfonamides, antimicrobial agents have been shown to shorten the duration of symptoms and lessen the excretion of pathogens during episodes of shigellosis. not all antimicrobial agents that are active in vitro against shigella are effective in vivo, and efficacy of an agent can only be assessed by properly conducted clinical trials. resistance to both ampicillin and trimethoprim-sulfamethoxazole, the drugs of choice for t ...19912047659
site-specific recombinase genes in three shigella subgroups and nucleotide sequences of a pinb gene and an invertible b segment from shigella boydii.inversional switching systems in procaryotes are composed of an invertible dna segment and a site-specific recombinase gene adjacent to or contained in the segment. four related but functionally distinct systems have previously been characterized in detail: the salmonella typhimurium h segment-hin gene (h-hin), phage mu g-gin, phage p1 c-cin, and escherichia coli e14 p-pin. in this article we report the isolation and characterization of three new recombinase genes: pinb, pind, and defective pinf ...19912061288
[a provisional serovar of shigella dysenteriae isolated in japan from travellers' diarrhea].a bacterial strain was isolated that possessed the biochemical characteristics of shigella dysenteriae but did not belong to any of the established s. dysenteriae serovars, from the feces of a diarrheal case who had travelled to turkey, pakistan, india and thailand for about a month. this strain was invasive to tissue culture cells and gave a positive serény test. preparing antiserum against this strain and examining the antigenic relationship with the established o antigens of shigella and esch ...19912066600
structural equation modeling in clinical assessment research with children.the use of structural equation modeling has gained increased interest in recent years in the social and behavioral sciences. this article reviews the basic tenets of structural modeling in relation to issues in research and practice involving clinical assessment and compares this approach with more traditional psychometric approaches to the validation of assessment instruments with children. arguments for and against the inclusion of nonexperimental variables in causal studies aimed at establish ...19812071721
nonsquamous tumors of the head and neck in the geriatric population.thyroid tumors, salivary gland tumors, and non-hodgkin's lymphoma are described. psychosocial issues involving the geriatric patient are discussed. case reports of a number of geriatric tumor patients are included.20072074986
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...19902075365
[the drug resistance and dna plasmid profiles of shigella dysenteriae 1 in the ussr and abroad].the medicinal resistance and plasmid profiles of 62 s. dysenteriae strains 1, isolated in the ussr in 1986-1988 from soviet and foreign citizens (from afghanistan, vietnam) and 8 strains obtained from india in 1987 were studied. pronounced similarity between the phenotypes of medicinal, including conjugative, resistance in the strains of soviet and foreign origin was established. in the soviet s. dysenteriae strains 1 the presence of two main types of plasmid dna profiles (140, 6, 4, 2 md and 14 ...19902075761
shiga-like toxin and haemolytic-uraemia syndrome. 19902080009
[the visit of french military physicians to the ussr]. 19552082543
[effect of maternal virus-specific t-lymphocytes on pregnancy and fetal pathogenesis in experimental congenital influenza infection].inoculation of virus-specific cytotoxic lymphocytes (ctl) to pregnant mice infected with influenza virus was shown to result in a decrease of infectious virus concentration in the lungs and blood of mice as well as in the placentas and fetuses. with an increase in the dose of inoculated ctl, however, premature deliveries with stillbirths were observed as well as deaths of the mice, the highest frequency of fetal deaths being observed after infection of pregnant mice in the first half of pregnanc ...19902082546
[effects of ethidium bromide on the viscosity of chromatin secreted by rat liver in normal state and after denervation].the paper studies structural state of chromatin secreted from the rat's liver. for this purpose the authors studied the dependence of relative viscosity of chromatin preparations secreted from the liver of rats and three groups of animals being in different terms of postdenervation process.19902083357
the mechanism of inhibition of s-adenosyl-l-homocysteine hydrolase by fluorine-containing adenosine analogs.(z)-4',5'-didehydro-5'-deoxy-5'-fluoroadenosine (i), 5'-deoxy-5'-difluoroadenosine (ii), and 4',5'-didehydro-5'-deoxy-5'-fluoroarabinosyl-adenosine (iii) are inhibitors of rat liver s-adenosyl-l-homocysteine hydrolase. compounds i and ii are time-dependent and irreversible inhibitors of the enzyme. both i and ii are oxidized by e.nad to produce e.nadh, and fluoride anion is formed in the inactivation reaction (0.7 to 1.0 mole fluoride/mole of enzyme subunit, and 1.7 moles fluoride/mole of enzyme ...19902094766
two unrelated cases of single maxillary central incisor with 7q terminal deletion.two unrelated cases of single maxillary central incisor (sm-ci) with 7q terminal deletion of the same breakpoint at 7q36.1 were described. they had mental retardation, microcephaly, hypotelorism, short stature, and normal levels of plasma growth hormone. one case had bilateral caudal ectopic kidneys, double renal pelves, and dilated ureters. the other had bilateral hydroureteronephrosis. the present cases suggest that 7q terminal deletion is one of the causes of smci.19902094780
[aminoacyl-trna synthetases (codases) and their noncanonical functions].the aim of this review is to summarize the data obtained in the author's laboratory during the last decade. the main objects of these investigations were mammalian aminoacyl-trna synthetases, mainly bovine tryptophanyl-trna synthetase (ec the data are discussed and compared with those described in literature. in the course of these studies it turned out that some properties of mammalian aminoacyl-trna synthetases for instance, nuclear location of some of the synthetases, presence of ex ...19902094804
[emergency coronary surgery in patients with unstable angina pectoris. results and place in the total concept of the treatment of this group of patients].the urgent coronary surgery performed in patients with high risk unstable angina (rest pain greater than 48 h) shows still different results. we found in 57 urgent operated patients the same functional and clinical results as after elective bypass grafting. a higher in hospital mortality after urgent coronary surgery based on a higher number of grafts are occluded in the early postoperative period. the better prognosis concluded that the use of urgent coronary surgery is the therapy of choice in ...20022094888
[identification of the public health nurse within the public health work force in the regional health department-6 of ribeirão preto, sp, (brazil)].the general purpose of this study was the identification of the nurse within the public health work force in the regional health department of ribeirão preto of the state health secretariat of s. paulo. a questionnaire the options of which included: identification, level of training, progress in career, function, future professional expectations and membership of professional associations, was the chief data source. of the group studies 35 were female, 40% of whom were between 20 and 39 years ol ...20032094953
[health economics--stopgap measures are no decisions. interview by kaj nyman]. 20062095080
["the profession is not the cause of the crisis"]. 20062095089
[the characteristics of the biological properties of shigella dysenteriae 1 circulating in the ussr and india: its biochemical activity and agglutinability].the properties of 71 s. dysenteriae 1 strains isolated from patients in the ussr and india in 1986-1988 were studied. the cultures possessed typical biochemical and serological properties. as revealed in this investigation, high fastidiousness of this infective agent to the quality of synthetic nutrient could become the cause of false negative reactions in different substrates used for the identification of enterobacteria, thus leading to diagnostic mistakes. the variability of the biochemical a ...19902097846
[derivative spectroscopy and its use in sanitary hygiene control].an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...20072099444
outbreak of dysentery due to nalidixic acid resistant s. dysenteriae 1 at agartala, tripura: a hospital based study.during the epidemic of bacillary dysentery at agartala, tripura, a total of 62 hospitalized patients suffering from diarrhoeal diseases were studied during the later part (11-16 june, 1988) of the epidemic. principal features of shigellosis cases were discussed. of these 62 cases investigated, 19(30.6%) cases had the mucoid diarrhoea. from them s.dysenteriae type 1 and s.flexneri had been recovered from 31.6% and 10.5% cases, respectively. all the strains of s.dysenteriae type 1 isolated during ...19902101382
multidrug resistant epidemic shigellosis in a village in west bengal, out break of acute bacillary dysentery in a village called dhamasin in hooghly district of west bengal was investigated during march 1984. forty seven percent of families were affected. a total of 91 cases and 2 deaths occurred amongst 937 people giving an over all attack rate of 9.7% and a case fatality rate of 2.2 percent. highest attack rate (22.7%) was observed in below one year age group. multiple drug resistant shigella dysentery type 1 strains were isolated for the first time from 6 ou ...19902101383
construction and evaluation of live attenuated vaccine strains of shigella flexneri and shigella dysenteriae 1.shigellosis is an invasive disease of the human colon which is particularly prevalent among children of the developing world. no proper vaccine is available to protect against this enteric disease. it is currently accepted that only live strains with attenuated virulence administered orally may elicit protective immunity at the level of the colonic mucosa, which is the exclusive site of multiplication of causative microorganisms such as shigella flexneri and shigella dysenteriae 1. we have const ...19902101481
improved sectioning and ultrastructure of bacteria and animal cells embedded in lowicryl.lowicryl k4m-embedded gram-positive and gram-negative bacteria have a tendency to separate between the cell surface and the resin. this often leads to distortion of bacteria and more especially of mycobacteria. we describe attempts made to overcome this technical problem. different assays were made on bacillus subtilis, escherichia coli, and mycobacterium avium: 1) modification of the bacterial surface by coating of bacteria with proteinic compounds; 2) treatment of bacteria with metallic salts ...19902110246
[experience in using new drug preparations for treating adult patients with acute intestinal infections].ciprofloxacin (bayer, frg), a derivative of hydroxyquinolone acid, was used for the treatment of patients with shigellosis and salmonellosis and for the sanitation of salmonella carriers. the drug turned out to exert a positive effect on bacteriological sanitation of the body. in order to treat patients with food toxinfection of unknown etiology, use was made of intetrix (farmacos, socialist federal republic of yugoslavia). inclusion of the drug into combined treatment of patients with food toxi ...19902110389
possible torsade in a patient with aids. 19892115530
immunodetection of lipopolysaccharide in macrophages during the processing of non invasive shigella dysenteriae.the location of lipopolysaccharide (lps) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive shigella dysenteriae 1 strain. bacterial degradation began only 3 h after the end of infection. the first visible sign of degradation was detected by immunogold labelling at the level of lps which detached from the bacterial surface and was transferred to the perinuclear lysosomes. after a few hours, it was found in small vesicles spread over the wh ...19902129020
effective induction immunosuppression for cadaver renal transplantation at the st. francis regional medical center.the okt3 induction protocol, when compared to a conventional drug protocol, resulted in an improvement in both short- and long-term patient and graft survival, especially in patients receiving a primary transplant. the 1-year allograft survival in primary transplants receiving okt3 induction was 91% and the t1/2 of 13.5 years. this was accomplished while still being able to minimize the total drug dose of aza, pred, and csa, thereby minimizing clinical complications. the use of okt3 did not incr ...19852129408
[a scapular osteo-cutaneous flap in mandibular reconstruction].the authors report their experience with 6 cases involving the scapular t.o.l.v. technique described by swartz et coll. they review the anatomy of the vascularisation of the flap together with the possible operative techniques. the 6 cases involved mandibular reconstruction following transmandibular bucco-pharyngectomy, either as first or second line surgery. in all cases the osteo-cutaneous flap gave a good quality reconstruction both in terms of oro-bucco-pharyngoplasty and in terms of repair ...19732130448
training of specialists for the biomedical sciences in the ussr. 19982132951
kinetic analysis of 3-quinuclidinyl 4-[125i]iodobenzilate transport and specific binding to muscarinic acetylcholine receptor in rat brain in vivo: implications for human studies.radioiodinated r- and s-quinuclidinyl derivatives of rs-benzilate (r- and s-125iqnb) have been synthesized for quantitative evaluation of muscarinic acetylcholine receptor binding in vivo. two sets of experiments were performed in rats. the first involved determining the metabolite-corrected blood concentration and tissue distribution of tracer r-iqnb (active enantiomer) and s-iqnb (inactive enantiomer) in brain 1 min to 26 h after intravenous injection. the second involved the measurement of br ...19902134838
clonal deletion of self-reactive t cells at the early stage of t cell development in thymus of radiation bone marrow chimeras.sequential appearance of t cell subpopulations occurs in the thymocytes of irradiated c3h/he mice (h-2k, mls-1b2a, thy-1.2) after transplantation with bone marrow cells of akr/j mice (h-2k, mls-1a2b, thy-1.1) (akr----c3h chimeras). the donor-derived thymocytes of akr----c3h chimeras on day 14 after bone marrow transplantation (bmt) contained a large number of blastlike cd4+cd8+ cells which represent relatively immature thymocytes, whereas those on day 21 after bmt consisted of small sized cd4+,c ...20042141619
effects of chronic non-ketotic diabetes and aging on myocardial function and fatty acid oxidation.the effects of aging and chronic non-ketotic diabetes on contractile properties, oxygen consumption, palmitate oxidation and morphology were studied in isolated, perfused working hearts of 2, 9, 12 and 22 month old rats. the heart rate, coronary flow, and oxygen consumption were no different among the 9, 12 and 22 month control and diabetic hearts. cardiac work was not depressed in control hearts until 22 months of age. depression of cardiac output due to aging in the control hearts progressed i ...19902141842
[antibiotic resistance of shigella isolated in the kara-kalpak a.s.s.r. 1987-1988].sensitivity of shigella spp. isolated in one of the hospitals of nukus within 1987-1988 and earlier in 1977 and 1985 was studied. s. flexneri 1-5 remained the main causative agents of dysentery on the territory. however, beginning from 1987 there were registered cases of dysentery caused by s. dysenteriae 1. the isolates were most sensitive to cefotaxime, cephaloridine, polymyxin b and gentamicin. the majority of the isolates were resistant to tetracycline, levomycetin (chloramphenicol) and stre ...19902144726
transposition in shigella dysenteriae: isolation and analysis of is911, a new member of the is3 group of insertion sequences.twenty-nine clear-plaque mutants of bacteriophage lambda were isolated from a shigella dysenteriae lysogen. three were associated with insertions in the ci gene: two were due to insertion of is600, and the third resulted from insertion of a new element, is911. is911 is 1,250 base pairs (bp) long, carries 27-bp imperfect terminal inverted repeats, and generates 3-bp duplications of the target dna on insertion. it was found in various copy numbers in all four species of shigella tested and in esch ...19902163395
the antiphospholipid antibody dilemma. 20072168305
associations of escherichia coli k-12 ompf trimers with rough and smooth lipopolysaccharides.the associations of both rough and smooth lipopolysaccharides (lps) with the ompf porin of escherichia coli k-12 were examined in gale strains deleted for ompc. transformation with pss37 and growth with galactose conferred the ability to assemble a shigella dysenteriae o antigen onto the core oligosaccharide of e. coli k-12 lps. the association of lps with ompf trimers was assessed by staining, autoradiography of lps specifically labeled with [1-14c]galactose, and western immunoblotting with a m ...19902168378
neutralization of poliovirus by cell receptors expressed in insect examine the interaction of the poliovirus receptor (pvr) with virus and the role of the pvr in virus entry, the pvr was expressed in insect cells. poliovirus bound to insect cells infected with a recombinant baculovirus (acpvr) carrying cdna encoding the pvr. antibodies raised against pvr expressed in bacteria immunoprecipitated a 67-kilodalton polypeptide from cytoplasmic extracts of acpvr-infected cells. treatment of acpvr-infected cells with tunicamycin revealed that the pvr is a glycoprot ...19902168959
[shiga and shiga-like toxins]. 19902173117
a model for evaluation of gas exchange: mouth to mouth ventilation of infants by emergency medical technicians.we describe a model for evaluating techniques of infant ventilation during resuscitation. the utility of the model is illustrated by testing performance of emergency medical technicians in mouth to mouth ventilation of a model 4 kg infant. ventilation was generally adequate with mean (+/- s.d.) frequency 22 +/- 9 breaths per minute and tidal volume 40 +/- 13 ml. gas delivered to the model consisted of pico2 7 +/- 6 mmhg and fio2 0.20 +/- 0.007. assuming normal metabolic rate and respiratory dead ...20082174185
trimethoprim resistance gene in shigella dysenteriae 1 isolates obtained from widely scattered locations of asia.trimethoprim-resistance genes of shigella dysenteriae 1 strains, isolated from a different location of six different countries of asia over a 5-year period were characterized by using three different dihydrofolate reductase (dhfr) gene probes. the trimethoprim-resistant (tmpr) strains hybridized only with the type i dhfr gene probe by colony hybridization. none of the strains hybridized with types ii and iii dhfr gene probes. southern blot experiments using plasmid dna extracted from these resis ...19902182327
rapid and specific detection of verotoxin genes in escherichia coli by the polymerase chain reaction.a set of four synthetic oligonucleotide probes derived from sequences of the vt1 (shiga-like toxin i [slt-i]) and vt2 (slt-ii) genes were used in a polymerase chain reaction (pcr) amplification procedure to detect these genes in some enteric pathogens. a total of 40 verotoxin-producing escherichia coli strains and 43 isolates of other recognized enteric pathogens were studied. pcr amplification products identifying the vt1 and vt2 gene sequences were observed only in nucleic acid extracted from ...19902182671
imaging the carpal tunnel. 20012190578
[antibiotic resistance of strains of shigella dysenteriae and flexneri isolated in tananarive and on the east coast of madagascar].from november 1988 to march 1989, stools from 804 malagasy children were examined, thirty-seven strains of shigella were isolated, from which 5 s. dysenteriae serotype 1 (= shiga bacillus) strains were found to be resistant to ampicillin, carbenicillin, streptomycin, chloramphenicol, tetracycline, sulphonamide and even to trimethoprim. resistance to trimethoprim has appeared only recently in madagascar.19902190702
dna sequence analysis of pgla and mechanism of export of its polygalacturonase product from pseudomonas solanacearum.the pgla gene encodes a 52-kilodalton extracellular polygalacturonase (pga) which is associated with the phytopathogenic virulence of pseudomonas solanacearum. the nucleotide sequence of pgla and the putative amino acid sequence of the pga protein were determined. a computer search identified a 150-residue region of pga which was similar (41%) to the amino acid sequence of a region of the pg-2a polygalacturonase from tomato. comparison of the amino terminus of the pgla open reading frame with th ...19902193922
comparative study of attachment to and invasion of epithelial cell lines by shigella dysenteriae.henle 407 and hela cells were compared as hosts for shigella dysenteriae at a low multiplicity of infection. efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic s. dysenteriae without congo red, were much greater for henle 407 cells than for hela cells.19902194970
production of shiga-like toxin among escherichia coli strains and other bacteria isolated from diarrhea in são paulo, elevated level of shiga-like toxin i (slt-i) production was found in 1 of 466 escherichia coli strains studied. among the 34 sonic lysates obtained from classical enteropathogenic e. coli, 5 produced slt-i. the aeromonas, citrobacter, edwardsiella, enterobacter, klebsiella, proteus, providencia, pseudomonas, salmonella, serratia, shigella, yersinia, and vibrio strains also studied were not slt producers, except for a shigella dysenteriae type 1 strain. although slt-i-producing e. coli strains ...19902199511
polymerase chain reaction for detection of invasive shigella flexneri in food.the polymerase chain reaction (pcr) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive escherichia coli, shigella flexneri, shigella dysenteriae, shigella boydii, and shigella sonnei as templates. this pcr product was easily detected by agarose gel electrophoresis. a 210-bp acci-psti fragment lying within the amplified region was used as a probe in southern hybridization blots and showed that the pcr-generated product was derived from the invas ...19902200336
epidemiology of shigellosis in teknaf, a coastal area of bangladesh: a 10-year survey.the epidemiological data on shigellosis in teknaf, a coastal area of bangladesh, were reviewed for a 10-year period (1975-84). certain similarities and differences were observed in the epidemiology of the disease in teknaf when compared with urban dhaka and rural matlab. similarities included: round-the-year infection with two peaks, one in the monsoon period and the other in the winter period; high male to female attendance ratio at the treatment centre; the predominance of infection in the und ...19902200700
plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from an epidemiological survey, plasmid profiles and antimicrobial susceptibility testing of 100 shigella isolates in lagos, nigeria was done. all the isolates were sensitive to nalidixic acid, nitrofurantoin and ciprofloxacin. the commonest antimicrobial susceptibility pattern was resistance to ampicillin, colistin sulphate, co-trimoxazole, streptomycin and tetracycline. all but 4 of 100 isolates screened contained one or more plasmids. plasmid profile analysis distinguished more strains than did ...19902200702
plasmid profiles of antibiotic-resistant shigella dysenteriae types 2, 3, 4, 6 and 7 isolated in ethiopia during 1976-85.plasmid profile analysis by agarose gel electrophoresis was carried out on 37 drug-resistant strains of shigella dysenteriae types 2, 3, 4, 6 and 7. these strains were collected between 1976 and 1985 in addis ababa, ethiopia. the plasmid profile of s. dysenteriae type 2 strains with r-type cssut did not show middle-sized plasmids likely to code for cssut resistance. all strains contained a large plasmid of about 120 megadaltons (mda), and a cryptic plasmid of about 2.2 mda. the plasmid profiles ...19902200703
haemolytic uraemic syndrome with shigella.a 49-year-old male developed bloody diarrhoea whilst on a visit to india. sigmoidoscopy and rectal biopsy showed acute colitis. shigella dysentery type i was isolated from stool culture. cytotoxin production by the organism was demonstrated. the patient developed acute renal failure, thrombocytopaenia and microangiopathic haemolytic anaemia. he required mechanical ventilation, haemodialysis, blood transfusion and antibiotic therapy and achieved a complete recovery. this is an unusual case of hae ...19902202698
in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i.the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ...19902203313
carboxy-terminal peptides from the b subunit of shiga toxin induce a local and parenteral protective effect.two synthetic peptides corresponding to overlapping sequences from the c-terminus of the b chain of shiga toxin were prepared and characterized. these peptides consisted of residues 54-67 and 57-67 in the protein sequence. this region coincides with the major peak of surface area residues, as predicted from a computer-derived plot. for the purpose of immunization, the peptides were either conjugated with a protein or a synthetic carrier, or were polymerized. polyclonal antibodies against these p ...19902203962
new approach to develop a vaccine design against shigellosis. 19902204244
recent advances in understanding the pathogenesis of the hemolytic uremic of the requirements for an agent to cause hemolytic uremic syndrome (hus) is its ability to injure endothelial cells. shiga-like toxin (slt) can do this. slt is produced by escherichia coli and shigella dysenteriae serotype 1; both have been implicated as causes of typical hus. endothelial cells have receptors (gb3) for slt and the toxin can inhibit eukaryotic protein synthesis, thereby causing cell death. glomerular endothelial cell injury or death results in a decreased glomerular filtrati ...19902205273
[a case of shigellosis due to shigella dysenteriae 1 which was thought to be infected in domestic area].a 43-year-old man was admitted to our hospital because of bloody diarrhea and abdominal pain on january 10, 1989. on laboratory examination s. dysenteriae 1 was isolated from his stool, therefore he was diagnosed as shigellosis. after the administration of ofloxacin was started, s. dysenteriae 1 was immediately eradicated. but his diarrhea persisted until the 19th day from the onset of the illness and his abdominal pain persisted until the 21st day. he had never been overseas and he had never ea ...19902205674
[detection of two shiga-like toxins from escherichia coli o157:h7 isolates by the polymerase chain reaction method].fourteen isolates of e. coli o157:h7 and five isolates of s. dysenteriae type-1 were examined by polymerase chain reaction (pcr) for the structural genes (slt-i or slt-ii), encoding shiga-like toxins (slts). the two primer pairs (v1; 5'agttaatgtggtggcgaa and v2; 5'gactgcgtcagtgaggtt for slt-i, v3; 5'ttcggtatcctattcccg and v4; 5'tctctggtcattgtatta for slt-ii) used were of the same positions representing the dna sequence covering 471bp of the slt-i or slt-ii. a 5-microliter portion of boiled bacte ...19902205737
cell surface proteins from shigella dysenteriae type 1.a simple extraction procedure was used for preparing cell surface proteins (csps) from shigella dysenteriae type 1. the preparations obtained using either buffer or water extractions were free from lipopolysaccharide (lps), as well as cytoplasmic and periplasmic proteins. by sds-page, about 25 polypeptides were detected, and western-blot analysis recognised 15 polypeptide antigens. when analysed by crossed immunoelectrophoresis, using anti-shigella dysenteriae type 1 rabbit sera, 18 antigenic ba ...19902206198
synthesis and biological activity of 3-vinylthio- and 3-vinylthiomethylcephem derivatives.the synthesis and biological properties of some 3-vinylthio- and 3-vinylthiomethylcephem derivatives are described. both series possess potent antibacterial activity. among them, 3-[(z)-2-cyanovinylthiomethyl]cephem derivative was found to have an expanded antibacterial spectrum.20082211378
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