| childhood carriers of salmonella and shigella species in the rural area of ile-ife, nigeria. | four hundred and eight apparently healthy school children from rural primary schools in ile-ife were examined for the prevalence of salmonella and shigella species, which were found respectively in 2% and 3.2% of these children. the prevalence of the organisms decreased as the age of the children increased. salmonella species were found only in specimens from boys while shigella species occurred in both sexes and were more prevalent among females. no specimen contained both organisms. only one o ... | 1990 | 2364932 |
| [aeromonas species as cause of diarrhea and infections outside the gastrointestinal tract in the netherlands]. | in a national survey in the period may 1986-december 1987, aeromonas was isolated from 277 out of 16,857 (1.6%) samples of watery, bloody or mucous stool, from patients with diarrhoea. there was a clear seasonal pattern (less than 1% in winter, up to 3% in summer). a. caviae was isolated most frequently (49%), followed by a. sobria (35%) and a. hydrophila (15%). some non-identifiable strains were isolated as well. aeromonas were isolated in particular from faeces of patients aged over 70 years ( ... | 1990 | 2366898 |
| [comparative study of the antibacterial activity of cefpodoxime against enterobacteria producing beta lactamases]. | cefpodoxime proxetil (ru 51 807) is the oral prodrug of cefpodoxime (ru 51 763), a third generation cephalosporin. the antibacterial activity of cefpodoxime was compared with the activities of amoxicillin in combination with clavulanic acid (aug), cefaclor (ccl), cefuroxime (cxm) and cefotaxime (ctx), against species of enterobacteriaceae showing a resistance pattern against ampicillin (amp), ticarcillin (tic), cefalothin (cft) and cefotaxime (ctx) respectively. for strains amp and tic r, cft an ... | 1990 | 2367149 |
| role of bacterial and host factors in the pathogenesis of shigella septicemia. | to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ... | 1990 | 2373088 |
| microscopic examination of stools and a latex slide agglutination test for the rapid identification of bacterial enteric infections in khmer children. | eighty-five stools collected from 50 children with diarrhea at an evacuation site on the thai-kampuchean border were (1) examined microscopically for fecal leukocytes, (2) tested after 24 hr enrichment in brain/heart infusion broth by a latex slide agglutination test for detection of salmonella and shigella, and (3) examined with microbiological techniques to identify bacterial, viral, and parasitic pathogens. if the 65 specimens in which one or no pathogens are considered, 6 or more fecal leuko ... | 1990 | 2383976 |
| studies on transformation in shigella. | transformation of pbr322 dna into shigella occurred at a low frequency. the efficiency of transformation was highest in s. dysenteriae 1 and lowest in s. flexneri. treatment of cells with cacl2 for a prolonged period (24h) increased the efficiency of transformation in all strains, except in s. flexneri, where transformation efficiency could not be improved by a variety of manipulations. transformation efficiency did not increase in any of the strains when transformation was carried out with plas ... | 1990 | 2390745 |
| a method for detecting shiga toxin and shiga-like toxin-i in pure and mixed culture. | shiga toxin and shiga-like toxins (slts, syn. verotoxins) are currently detected by tissue culture assays that are expensive, time-consuming and require specialised facilities and experienced personnel. we have developed a rapid method to detect shiga toxin and slt-i (verotoxin 1) based on their binding to globotriosyl ceramide (gb3). bound toxin was then detected by an enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies. the direct detection of cytotoxins from pure culture plat ... | 1990 | 2391709 |
| phosphorylation of the virg protein of agrobacterium tumefaciens by the autophosphorylated vira protein: essential role in biological activity of virg. | agrobacterium tumefaciens virulence genes are induced by plant signals through the vira-virg two-component regulatory system. the vira protein is a membrane-spanning sensor molecule that possesses an autophosphorylating activity, and the virg protein is a sequence-specific dna-binding protein. in this report, we demonstrate that the virg protein is phosphorylated by the vira protein and that the phosphate is directly transferred from the phosphorylated vira molecule (phosphohistidine) to the vir ... | 1990 | 2394678 |
| trimethoprim resistance; epidemiology and molecular aspects. | | 1990 | 2404123 |
| doxycycline prophylaxis for shigellosis. | the efficacy of oral doxycycline, 100 mg/d for 14 days, in reducing the incidence of shigellosis in newcomers to an area hyperendemic for the disease was examined in a double-blind, placebo-controlled trial. of 107 entrants, 100 completed the study; 50 received the drug and 50 received a placebo. diarrheal disease and associated symptoms were monitored for 8 weeks. starting on the 3rd day of the trial, an outbreak was observed, and shigella flexneri type 2a was isolated from 6 subjects. eight of ... | 1990 | 2404480 |
| contribution of superoxide dismutase and catalase activities to shigella flexneri pathogenesis. | a shigella flexneri serotype 5 strain deficient in the production of the iron-containing superoxide dismutase fesod (sodb) and a catalase-negative (katfg) s. flexneri serotype 5 strain were isolated. both strains were examined for increased sensitivity to oxygen stress by using assays involving killing by mouse peritoneal macrophages and human polymorphonuclear leukocytes as well as infection of rabbit ileal loops. the sodb mutant was extremely sensitive to killing by phagocytes when compared wi ... | 1990 | 2404874 |
| functional analysis of the shiga toxin and shiga-like toxin type ii variant binding subunits by using site-directed mutagenesis. | the b subunit of shiga toxin and the shiga-like toxins (slts) mediates receptor binding, cytotoxic specificity, and extracellular localization of the holotoxin. while the functional receptor for shiga toxin, slt type i (slt-i), and slt-ii is the glycolipid designated gb3, slt-ii variant (slt-iiv) may use a different glycolipid receptor. to identify the domains responsible for receptor binding, localization in escherichia coli, and recognition by neutralizing monoclonal antibodies, oligonucleotid ... | 1990 | 2404947 |
| acute glomerulonephritis due to shigella flexneri dysentery with demonstration of a virulence protein of shigella in circulating immune complexes. | | 1990 | 2405072 |
| the use of hygiene, cohorting, and antimicrobial therapy to control an outbreak of shigellosis. | shigellae are easily transmitted in day-care centers to children and adult staff by contamination of diaper-changing surfaces and fomites or directly from person to person. appropriate antimicrobial therapy may shorten the duration of diarrhea caused by shigellae and eliminate the organism from the feces. current recommendations of the american academy of pediatrics and the american public health association are that infected children be isolated until three and two, respectively, consecutive st ... | 1990 | 2405639 |
| aetiological studies of diarrhoeal diseases in infants and young children in iran. | the aetiology of diarrhoeal diseases was investigated among 715 patients admitted to four children's hospitals in tehran, during february 1986 to march 1987, and also among 443 patients attending the central out-patient clinic in sanandaj, state of kordestan, during october 1986. enteropathogenic escherichia coli (epec) were the most common pathogens found in both areas. almost 26.7% of the patients in tehran and 20.1% of the patients in sanandaj were infected with epec. enterotoxigenic e. coli ... | 1990 | 2406458 |
| in vitro antimicrobial activity of bismuth subsalicylate and other bismuth salts. | this report demonstrates that bismuth subsalicylate (bss) effectively inhibits growth of a number of bacterial strains known to cause diarrhea, including escherichia coli, salmonella, shigella, and campylobacter. other bismuth salts and sodium salicylate, a hydrolysis product of bss in the gut, also were examined and were shown to have various degrees of activity. growth of the organisms was monitored in vitro by inoculating culture fluid that contained one of the compounds to be tested and dete ... | 1990 | 2406851 |
| diarrhea in developed and developing countries: magnitude, special settings, and etiologies. | diarrheal diseases are major causes of morbidity, with attack rates ranging from two to 12 or more illnesses per person per year in developed and developing countries. in addition, diarrheal illnesses account for an estimated 12,600 deaths each day in children in asia, africa, and latin america. the causes of diarrhea include a wide array of viruses, bacteria, and parasites, many of which have been recognized only in the last decade or two. while enterotoxigenic escherichia coli and rotaviruses ... | 1990 | 2406855 |
| epidemiology of travelers' diarrhea and relative importance of various pathogens. | each year 12 million persons travel from an industrialized country to a developing country in the tropics or subtropics. these travelers experience a high rate of diarrhea caused by a wide variety of enteric pathogens acquired by ingestion of contaminated food or water. one or more pathogens can be found in the stool of a majority of ill individuals. enterotoxigenic escherichia coli generally are the most frequently identified pathogens, having been found in a median of 42% of travelers' diarrhe ... | 1990 | 2406860 |
| the colon in shigellosis: serial colonoscopic appearances in shigella dysenteriae i. | twenty-five patients (16 males and 9 females; mean age 24.7 +/- 8.4 years) with acute colitis caused by shigella dysenteriae i were studied, a total of 115 colonoscopic examinations being performed. twenty-two patients had weekly colonoscopic examinations until the last procedure revealed normal colonic mucosa. six abnormal colonoscopic appearances were documented, namely mucosal edema, ulcers, friability, punctate spots, erythematous areas and luminal exudate. mucosal edema was the dominant fin ... | 1990 | 2407526 |
| [theoretical conformation analysis of tetrasaccharide-repeated links of the shigella flexneri o-antigenic polysaccharide]. | theoretical conformational analysis of four tetrasaccharide repeating units of the shigella flexneri serogroup y polysaccharide has been carried out. interdependency of conformational states of neighbouring disaccharide units in the oligosaccharides has been investigated and conformational distribution of tetrasaccharides in solution calculated. taking into account the entropy of oligosaccharide chains is shown to lead to significant correction of the results. | 1985 | 2408628 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| vertical transmission of enteric pathogens at birth. | to determine how frequently mothers infect their infants with enteric pathogens at birth, stools were collected from 75 thai mothers immediately before delivery and from their infants 24-48 h later. enteropathogenic escherichia coli (epec) were isolated from 25 of the 75 mothers just prior to delivery (32%), enteroviruses from six (8%), enterotoxigenic e. coli from five (7%), and shigella, salmonella and vibrio parahaemolyticus each from one (1%). epec serotype 0128:k67 was isolated from one mot ... | 1985 | 2409892 |
| use of non-carbonated soft drinks to provide safe drinking water. | non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ... | 1985 | 2409896 |
| experiences with serology of plesiomonas shigelloides. 1. o-antigenic structure. | with a set of 30 o-antisera, o-antigens were identified in 80% of 158 plesiomonas shigelloides strains. only strains of one serovar (018) regularly contained capsular antigen that caused their inagglutinability in the live state. two groups of serovars displayed some o-antigenic relationship: 03 and 029; 08, 011 and 012. each serovar in either group possessed a specific o-antigen and "group-common" minor antigens, which were designated i, ii and iii. serovar 017 possessed o-antigen identical wit ... | 1985 | 2410492 |
| axonally transported shigella cytotoxin is neuronotoxic. | shigella dysenteriae strains produce an exotoxin (sdt) which inhibits protein synthesis in susceptible cells and is neurotoxic in some species. intraneural microinjection of highly purified sdt into the cervical vagus nerves of rats, mice, guinea pigs and rabbits produced cytopathic changes within 24 hours in vagal sensory but not motor neurons. these changes consisted of an initial loss of nissl substance followed by progressive cell degeneration and resulted in permanent neuronal loss. indirec ... | 1985 | 2411880 |
| [method for the statistical processing of the results of an immunoenzyme analysis]. | a method for the statistical processing of data having different distribution functions is proposed. this method consists in the use of integral distribution functions. the description of the method based on the use of soviet serial table-top microcomputers, model elektronika mk-56, with specially developed programs (primary statistical data processing and single-factor regression analysis) is presented. the possibilities of this method are illustrated, by way of example, by processing the resul ... | 1985 | 2412370 |
| [synthesis of oligosaccharide fragments of o-specific polysaccharides of shigella flexneri. iii. synthesis of the tetrasaccharide glc alpha 1-3rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome and the pentasaccharide glcnac beta 1-2(glc alpha 1-3)rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome]. | two synthetic schemes to prepare the title branched tetrasaccharide and pentasaccharide are described. these oligosaccharides represent fragments of the o-antigenic polysaccharides of shigella flexneri serotype 5b. | 1985 | 2412567 |
| [membrane activity of a cell-free filtrate of shigella flexneri 2a]. | in the course of metabolism in shigella flexneri 2a strain there was noted secretion of a membrane-tropic factor into media. this factor increased the conductivity of bilayer lipid membranes causing formation of ionic channels in them. minimal conductivity of the channels was about 25 ps in 0.5 m nacl; at ph 7-8 the channels exhibited cation-anion selectivity. the calculated number of cation transfer (t+) was elevated with an increase in the channel amplitude, approaching 0.86. since the conduct ... | 1985 | 2413620 |
| 'o'-antigens are essential virulence factors of shigella sonnei and shigella dysenteriae 1. | a novel transposon mutagenesis system has been developed for shigella. we have used it to isolate specific mutants of shigella sonnei and shigella dysenteriae 1 that fail to produce lipopolysaccharide 'o' side chains. the virulence of the mutants was evaluated in the sereny test and in a hela cell invasion assay. all ho'-minus mutants failed to provoke a positive sereny reaction but retained the ability to invade hela cells. this demonstrates that 'o' side chains are virulence factors of s. sonn ... | 1985 | 2413650 |
| inactivation by polymyxin b of the endotoxin-mediated interferon production in the rabbit. | polymyxin b (pb) completely or at least significantly inhibited the capacity of shigella dysenteriae 1 cells and the lipopolysaccharide (lps) and lipid a (la) subunits of several bacterial endotoxins to induce interferon (ifn) in rabbits. animals injected with lps inactivated by pb to the point of not inducing detectable ifn levels did not develop hyporesponsiveness to secondary ifn induction by a homologous inducer. it was concluded that pb inhibits the ifn-inducing capacity of endotoxin and it ... | 1985 | 2413651 |
| [use of immunoenzyme analysis for evaluating the specificity of the antibody recognition of the antigenic determinants of streptococcus group a]. | investigations carried out with the use of a celia system have revealed that antibodies in the sera of patients with primary erysipelas and antibodies in rabbit antiserum to the ribosomes of group a streptococcus specifically bind with adsorbed streptococcal ribosomes, recognizing the antigenic determinants of streptococcal ribosomes, which differ from those of individual gram-negative prokaryotes (escherichia coli, shigella sonnei, shigella flexneri, salmonella minnesota). the modified celia sy ... | 1985 | 2413662 |
| [immune response and immune tolerance to the o-antigen of shigella flexneri vi]. | the method for the determination of the number of cells synthetizing antibodies to s. flexneri vi o-antigen in the spleen of mice has been developed. primary immune response to this antigen has been studied with the use of the new method. immune response to the optimum immunogenic dose of o-antigen has a manifest variable character. the intensity of primary immune response has been shown to rise with the increase of the dose of o-antigen from 0.004 to 50 micrograms. the preliminary injection of ... | 1985 | 2413664 |
| genetic determinants of virulence in shigella and dysenteric strains of escherichia coli: their involvement in the pathogenesis of dysentery. | | 1985 | 2414072 |
| [serodiagnosis of dysentery using shigella diagnostic agents (synthetic colored and erythrocytic)]. | | 1985 | 2414530 |
| [experimental study of the sensitivity of the enzyme-labeled antibody reaction and the coagglutination reaction for detection of shigella and salmonella antigens in urine]. | | 1985 | 2414531 |
| [specific antigens of the causative agents and their antibodies in the circulating immune complexes in acute dysentery]. | the level of circulating immune complexes has been determined in 53 patients in the dynamics of the disease. for the first time circulating immune complexes have been found to contain shigella sonnei k-antigen and shigella flexneri o-antigen, as well as iga, igg and igm to shigella. shigella antigens can be detected from the first week of the disease, and their occurrence does not depend on the level of circulating complexes in patients blood serum. | 1985 | 2414952 |
| [effect of vitamins of the b group on the accumulation of complex antigens of dysentery bacteria in the process of deep cultivation]. | | 1985 | 2416151 |
| [comparative characteristics of antigenic conversion induced by shigella flexneri and escherichia coli 0129 phages]. | the interaction of s. flexneri converting phages pe5, p90 and fv with e. coli antigenic variant o129, e. coli o129 converting phage vb with the above antigenic variant and with s. flexneri y-variant was studied. phage pe5 and phage vb were found to induce the conversion of o-antigen in e. coli antigenic variant 0129 and in s. flexneri y-variant with the detection of antigens v and 7,8. phages p90 and fv induced no conversion of o-antigen. changes in the antigenic properties of convertants were c ... | 1985 | 2416154 |
| anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections. | several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ... | 1986 | 2421739 |
| [effect of levamisole immunocorrective therapy on the dynamics of infectious o-antigenemia and the clinical manifestations in dysentery patients]. | the immunocorrecting agent levamisole decreases the duration of infectious o-antigenemia, determined in the o-aggregate hemagglutination test and in the enzyme immunoassay, essentially accelerates the progress of convalescence and considerably decreases the possibility of the prolonged relapsing course of the disease in comparison with common methods of treatment. | 1986 | 2422855 |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type i and type iii:6,7,8 antigens, group 6 antigen, and a core epitope. | monoclonal antibodies against the shigella flexneri lipopolysaccharide (lps) were generated in two fusions by using the myeloma cell line sp2/0 as a fusion partner with spleen cells from balb/c mice immunized with s. flexneri serotypes 1b and 3a bacteria. the antibodies were characterized by immunoblotting, enzyme-linked immunosorbent assay (elisa), hemagglutination, and coagglutination. four different types of monoclonal antibodies were isolated: antibodies specific for the core antigen of the ... | 1986 | 2424839 |
| shigella flexneri o-antigen epitopes: chemical and immunochemical analyses reveal that epitopes of type iii and group 6 antigens are identical. | in the present investigation we studied the nature of a shigella flexneri o antigen, the type iii antigen. no structural entity has yet been attributed to this antigen. phenol-water-extracted lipopolysaccharides (lpss) from all s. flexneri serotypes possessing either group 6 antigen or both type iii antigen and group 6 antigen were subjected to de-o-acetylation by weak alkali treatment. two-dimensional nuclear magnetic resonance spectra of serotype 3a native lps and de-o-acetylated lps were comp ... | 1986 | 2424840 |
| [nbt-test stimulation of neutrophilic granulocytes by bacterial preparations in healthy persons]. | | 1986 | 2425131 |
| hla-b27m1m2 and high immune responsiveness to shigella flexneri in post-dysenteric arthritis. | the heterogeneous hla-b27 antigen is closely associated with post-infectious or reactive arthritis (rea) and is comprised of two serologically defined variants: b27m1+m2+ and b27m1+m2-. an outbreak of dysentery (n = 120) caused by a shigella flexneri 2a strain, which possessed cell envelope antigens with epitopes resembling b27m2, resulted in five b27m1+m2+ patients with rea. the remaining seven b27m1+m2+, one b27m1+m2- and all but three b27-negative patients remained free of joint symptoms; the ... | 1986 | 2428743 |
| colonization, virulence, and mucosal interaction of an enteropathogenic escherichia coli (strain rdec-1) expressing shigella somatic antigen in the rabbit intestine. | the group factor 3,4 somatic antigen was transferred by recombination from a donor strain of shigella flexneri type 2a to the escherichia coli o15 rabbit pathogen strain rdec-1. a hybrid clone (7482-1-1) that expressed only the s. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the o15 somatic antigen were compared for virulence in rabbits. the 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (p les ... | 1986 | 2430026 |
| [genetic analysis of shigella plasmid involved in pathogenicity]. | | 1986 | 2430118 |
| [synthesis of a repeating unit and the dimer of the repeating unit of the major chain of shigella flexneri o-antigen polysaccharide]. | methyl glycoside of the tetrasaccharide glcnac(beta 1-2)rha(alpha 1-2)rha(alpha 1-3)rha, which represents a repeating unit of the basic chain of shigella flexneri o-antigenic polysaccharides, was synthesized using acylated monosaccharide synthons. a dimer of the repeating unit, octasaccharide [glcnac(beta 1-2)rha(alpha 1-2) rha(alpha 1-3)rha(alpha 1-3)]2-ome was obtained by trclo4-catalyzed condensation of two tetrasaccharide blocks. | 1986 | 2430581 |
| [somatic antigens of shigella and escherichia coli. determination of the structure of o-specific polysaccharide from shigella boydii type 12 and its serological properties compared with the o-specific polysaccharide from escherichia coli]. | the structure of the o-specific polysaccharide of the somatic antigen (lipopolysaccharide) of shigella boydii, type 12, was established by 1h- and 13c-nmr, methylation analysis and partial acid hydrolysis methods. the polysaccharide consists of pentasaccharide repeating units of the following structure: (formula; see text) the amount of o-acetyl groups was far less than stoichiometric, only about 2 for 3-4 repeating units. nevertheless, the results of serological studies revealed 3-o-acetyl-alph ... | 1986 | 2430583 |
| lack of correlation between hla-b27 like antigenic epitopes on shigella flexneri and the occurrence of reactive arthritis. | | 1986 | 2430745 |
| [intergeneric antigenic relation of hafnia and shigella (s. flexneri and s. boydii) bacteria]. | the o-antigenic relationships between hafnia and shigellae (s. flexneri and s. boydii) have been studied. for the first time the presence of antigenic relationship between hafnia o19, o4, o9, o33, o5, o16, o12, o7, o29, o28, o10, o32, o24, o25, o18, o1, o13, o3, o22, o30, o37, o14, o11, o25, o23, o21, o28, o16, o24, o8, o26, o27 and s. flexneri la, lb, 2a, 2b, 4a, 4b, 6, 5a, 5b, as well as between hafnia o10, o21, o35, o36, o9, o28, o8, o30 and s. boydii 1, 3, 6, 14, 2, 5, 2, 12 have been reveal ... | 1986 | 2431565 |
| a murine monoclonal antibody specific for the outer core oligosaccharide of salmonella lipopolysaccharide. | immunoglobulin g3 murine monoclonal antibody t6 specific for the lipopolysaccharide of salmonella o serogroups a to e was established. by using r mutants of salmonella spp., escherichia coli, and shigella spp., the major reactive epitope with t6 was tentatively identified as the terminal disaccharide, n-acetylglucosamine 1.2----alpha glucose, of the core oligosaccharide. t6 was reactive with 10 clinical isolates of each of the salmonella o serogroups a to e but not with 58 isolates of other gram ... | 1987 | 2432013 |
| analysis of cell division gene ftsz (sulb) from gram-negative and gram-positive bacteria. | the ftsz (sulb) gene of escherichia coli codes for a 40,000-dalton protein that carries out a key step in the cell division pathway. the presence of an ftsz gene protein in other bacterial species was examined by a combination of southern blot and western blot analyses. southern blot analysis of genomic restriction digests revealed that many bacteria, including species from six members of the family enterobacteriaceae and from pseudomonas aeruginosa and agrobacterium tumefaciens, contained seque ... | 1987 | 2432055 |
| enteropathic arthropathies. | musculoskeletal disease occurs in association with inflammatory bowel disorders including crohn's disease and ulcerative colitis, as well as with whipple's disease; with enteritis caused by salmonella, shigella, and yersinia; and also following intestinal bypass surgery. extraintestinal causes of musculoskeletal alterations include laennec's and biliary cirrhosis and pancreatitis. three types of musculoskeletal abnormalities are recognized in patients with inflammatory bowel diseases: peripheral ... | 1987 | 2434970 |
| [effect of rna nucleotides on shigella biology]. | sodium nucleinate and its mononucleotides have been found capable of stimulating the multiplication and virulence of shigellae, as well as their sensitivity to antibiotics, in cultures grown in nutrient media. | 1986 | 2435088 |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains. | monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ... | 1987 | 2437036 |
| prevention of shigellosis by a salmonella typhi-shigella sonnei bivalent vaccine. | we genetically modified attenuated salmonella typhi strain ty21a to express the form i o polysaccharide antigen of shigella sonnei. three doses of this bivalent, live oral vaccine strain (1-8 x 10(9) organisms/dose) were given to young adults who, along with unvaccinated controls, were challenged one month later with pathogenic s. sonnei. the vaccinees had 40% protection against diarrhea and 56% against hematest-positive diarrhea. two of three vaccine lots provided higher levels of protection (5 ... | 1987 | 2437220 |
| [genetic characteristics and trends in the spread of a new shigella flexneri subserovar with the antigenic formula iv:7,8]. | as the result of experiments with the conjugation of s. flexneri strains 4 belonging to an unusual subserovar (iv: 7,8) with escherichia coli donor strains k12 hfr c and hfr h, as well as experiments with converting phages iv and 7,8, this new subserovar of s. flexneri 4, similarly to other s. flexneri subserovars, was proved to be the y-variant of shigellae rendered lysogenic by the two above phages. the experiments also revealed that 97.9% of all s. flexneri strains 4 (iv: 7,8) under study pos ... | 1987 | 2437740 |
| [specificity of the serological diagnosis of dysentery using erythrocytic reagents]. | the serological specificity of the diagnosis of dysentery, made by different methods with the use of antigenic and antibody erythrocyte diagnostic agents under the conditions of the circulation of different shigella species and subspecies in a given locality, has been compared. the method for the determination of the diagnostic titer of the total serum antibody activity has proved to be the least specific. the methods for the detection of shigella antigens, especially over time, in patients' exc ... | 1987 | 2437742 |
| [antigenic determinants of bacteria. the structure of the repeating unit of a specific polysaccharide from shigella boydii type 7]. | acid hydrolysis of the antigenic lipopolysaccharide from shigella boydii type 7 afforded a specific polysaccharide composed of 2-acetamido-2-deoxy-d-glucose, d-glucose, d-galactose, 5-acetamido-3,5,7,9-tetradeoxy-7-[(3r)-3-hydroxybutyramido]-l- glycero-l-manno-nonulosonic acid (nonn2a) and acetic acid residues in the 1:1:2:1:1 ratio. from the results of methylation analysis, hydrogen fluoride solvolysis and smith degradation, the structure of the repeating unit of the specific polysaccharide was ... | 1987 | 2437931 |
| characterization of five shigella flexneri variant y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens. | the structural domains of the shigella flexneri variant y o-antigen epitopes 3,4 have defied definition, despite knowledge of the structure of the linear polysaccharide chain of the lps molecule. the dual epitope designation of group antigen 3,4 is based on absorption data using polyvalent rabbit antisera. five monoclonal antibodies specific for the y antigen, generated after immunization of balb/c mice or lou/c rats, were selected on the basis of elisa by using well-characterized s. flexneri y ... | 1987 | 2438343 |
| structure and biology of shigella flexneri o antigens. | | 1987 | 2438412 |
| antigenic specificity of escherichia coli alkaline phosphatase studied with monoclonal antibodies: immunological characterization of e. coli and shigella strains. | monoclonal antibodies (moab) to the alkaline phosphatase of escherichia coli were produced from spleen cells of balb/c mice primed with purified alkaline phosphatase of e. coli and sp2o/ag-14 myeloma cells. five stable clones were established. they all produced antibodies which reacted by enzyme-linked immunosorbent assay (elisa) with alkaline phosphatase of all e. coli (25 strains) independently of their origin (drinking water, saline water, surface water, faecal or clinical origin), and with t ... | 1987 | 2440463 |
| [nonspecific and antigen-specific adhesiveness of circulating lymphocytes in dysentery patients undergoing combined therapy using thymalin and indomethacin]. | nonspecific adhesiveness of lymphocytes to polyacrylonitrile fiber and the antigen-specific adhesiveness of t-lymphocytes to shigella flexneri pellicular immunosorbent have been studied in dysentery patients. short courses of treatment with indomethacin, but not with thymalin (the preparation of thymic hormones), used for immunomodulation, have accelerated the normalization of nonspecific lymphocyte adhesiveness during regression of the disease, while indomethacin, in contrast to thymalin, inhib ... | 1987 | 2441548 |
| aetiology and clinical features of acute childhood diarrhoea in an outpatient clinic in vellore, india. | the aetiology of acute diarrhoea was investigated in 245 children less than 3 years old in a paediatric outpatient clinic in southern india. in 55% of the children organisms were found in the stools, and one quarter were infected with multiple organisms. viruses, enteropathogenic e. coli, shigella and campylobacter species accounted for 75% of all isolates. cholera and helminthiasis were rare, and no child had amoebiasis. clinical findings were useful in the diagnosis of shigella and rotavirus i ... | 1987 | 2445263 |
| rotavirus as an important cause of diarrhoea in a hospital for children in dammam, saudi arabia. | a major cause of morbidity and mortality among children in developing countries is acute diarrhoea and rotavirus, a reovirus-like agent, is found to be a leading causative agent. we report here the incidence of rotavirus infection among infants and young children with gastro-enteritis treated at the children's hospital in dammam, kingdom of saudi arabia. one hundred and fifty children, aged between 1 and 60 months, suffering from diarrhoeal illness, were selected as the study cohort; another 150 ... | 1987 | 2445264 |
| [structure of the o-specific polysaccharide chain of shigella dysenteriae type 7 lipopolysaccharide]. | the structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide has been established mainly by 13c nmr analysis of the intact and modified (acetylated and de-o-acetylated) polymers, as well as of products of its solvolysis with anhydrous hydrogen fluoride. the polysaccharide contains two unusual sugar derivatives. n-acetyl-d-galactosaminuronamide and 4-(n-acetylglycyl)amido-4,6-dideoxy-d-glucose (galnacan and qui4n----glyac, respectively) and is built u ... | 1987 | 2445353 |
| the effects of the protein synthesis inhibitor anisomycin on the febrile responses to intracerebroventricular injections of bacterial pyrogen, arachidonic acid and prostaglandin e2. | 1. anisomycin (15 mg/kg) was administered s.c. to cats at ambient temperatures of 5 degrees c, 20 degrees c and 38 degrees c. it produced biphasic effects on body temperature at 5 degrees c and 20 degrees c, an initial fall in temperature followed by a rise in body temperature, and a rise in body temperature of long latency at 38 degrees c. 2. anisomycin (15 mg/kg) attenuated the hyperthermic responses to centrally injected pge2 (1 microgram) at all ambient temperatures studied and also complete ... | 1987 | 2446153 |
| polysaccharide antigens of escherichia coli. | the major surface antigens of escherichia coli are the cell wall lipopolysaccharides (lps; o antigens) and the capsular polysaccharides (ps; k antigens). these polysaccharides are synthesized at the cytoplasmic membrane of the bacteria; the lps are transported to the outer membrane, where they reside, whereas the ps are secreted into capsules. the lps consist of lipid a covalently linked to the core oligosaccharide, which itself is covalently linked to the o-specific polysaccharide. the latter, ... | 1987 | 2446369 |
| [antigenic polysaccharides of bacteria of the genus shigella. determination of the structure of polysaccharide chains of shigella boydii type 9 lipopolysaccharide and detection of unusually high molecular weight glycolipid]. | specific acidic polysaccharide has been isolated from the shigella boydii type 9 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of d-glucose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, and l-rhamnose. from the results of methylation analysis, partial acid hydrolysis and 13c nmr data the structure of the repeating unit of the polysaccharide was deduced as follows: [----4)dglcp(alpha 1----4)dglcap(beta 1----3)dglcn ... | 1987 | 2447896 |
| [antigenic polysaccharides of bacteria of the genus shigella. the structure of the polysaccharide chain of shigella boydii type 14]. | a specific acidic polysaccharide has been isolated from the shigella boydii type 14 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of the d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose and d-galactose residues in the ratio 1:1:3. from the results of methylation analysis and partial acid hydrolysis, the structure of the repeating unit of the specific polysaccharide was deduced as follows: (-6dgalp alpha 1-4dglcap beta ... | 1987 | 2447897 |
| [shigella toxicity in immunological tolerance]. | the toxic effect of killed and live shigella sonnei cultures on normal mice and on mice, tolerant to shigella o-antigen and to human erythrocytes of different blood groups (in the abo system) was under study. the toxicity of shigellae, introduced intraperitoneally, has been found to depend on their viability, on their capacity for penetration into the blood, and on the split character of immunological tolerance to shigella antigens. | 1987 | 2448979 |
| spectrum and mode of action of azithromycin (cp-62,993), a new 15-membered-ring macrolide with improved potency against gram-negative organisms. | the macrolide antibiotic azithromycin (cp-62,993; 9-deoxo-9a-methyl-9a-aza-9a-homoerythromycin a; also designated xz-450 [pliva pharmaceuticals, zagreb, yugoslavia]) showed a significant improvement in potency against gram-negative organisms compared with erythromycin while retaining the classic erythromycin spectrum. it was up to four times more potent than erythromycin against haemophilus influenzae and neisseria gonorrhoeae and twofold more potent against branhamella catarrhalis, campylobacte ... | 1987 | 2449865 |
| shiga toxin inhibits the anti-invasive effect of interferons. | | 1988 | 2450154 |
| [relation of the immunosuppressivity of virulent shigella to the structure of o antigen]. | the capacity of filtrates of virulent shigella cultures (vsc) and their different fractions to render avirulent strains, injected intraperitoneally into mice simultaneously with these filtrates, capable of suppressing immune response (delayed hypersensitivity) has been studied. among the fractions of vsc filtrates, the lipoid fraction soluble in the mixture of ethanol and ether has proved to be active. active factors can be extracted from vsc filtrates by means of immunosorbents obtained from an ... | 1988 | 2452538 |
| natural killer cell activation and interferon production by peripheral blood lymphocytes after exposure to bacteria. | we have previously shown that peripheral blood natural killer (nk) cells have significant levels of cytotoxic activity against shigella flexneri-infected hela cells. in this report, we show that nk cell activity against k562 tumor cells and shigella flexneri-infected hela cells can be greatly enhanced by preincubating peripheral blood lymphocytes (pbl) for 18 h with kanamycin-treated shigella flexneri or salmonella typhimurium. cell-free supernatants obtained from pbl-bacteria cultures contained ... | 1988 | 2453465 |
| [determination of the o antigen of shigella sonnei by a competitive immunoenzyme method]. | a technique for immunoenzymatic diagnosis of dysentery by shigella sonnei o-antigen was developed. for induction of antibodies to o-antigen rabbits were immunized by intravenous administration of a commercial antidysentery vaccine. specific antibodies to o-antigen belonging to class g immunoglobulins and not binding to o-antigens of sh. flexneri and salmonella typhimurium were obtained. beta-lactamase of bacillus licheniformis 749/c was used as a marker enzyme in the immunoenzymatic assay. to in ... | 1988 | 2454084 |
| the stability of o-antigen plasmid is determined by a chromosomal region of shigella dysenteriae 1. | it is well established that plasmids are involved in the expression of lipopolysaccharide in certain species of shigella. in shigella sonnei, both the biosynthesis of oligosaccharide side chains (o antigen), and cell invasiveness are controlled exclusively by a 120 megadalton (mda) plasmid. in shigella dysenteriae 1, a 10 kilobase (kb) plasmid is required for o-antigen production. shigella dysenteriae 1 strains devoid of this plasmid lose the ability to synthesize o antigen. interestingly, this ... | 1988 | 2454151 |
| [plasmid profile types of virulent and avirulent strains of phase-i shigella sonnei and their rough phase-ii and r-form variants]. | the study of s. sonnei in phase i, irrespective of their virulence, has revealed the existence of at least 3 types of profiles of large plasmids: (i)a having a single plasmid with a molecular weight of about 120 md; (i)b having, alongside plasmid pss120, a plasmid with a molecular weight of about 60 md; (i)c, represented only by vaccine strain 6s, having three plasmids with molecular weights of about 80, 60 and 37 md. the plasmid profiles of rough s. sonnei in phase ii are characterized by the a ... | 1988 | 2455394 |
| ribosomal-rna patterns of escherichia coli, salmonella typhimurium and related enterobacteriaceae. | rrna sequences are usually highly conserved among species. in enterobacteriaceae we have shown that salmonella typhimurium does not have an equivalent to the 23s rrna of escherichia coli but its 23s rrna is cleaved in vivo into two smaller species. this cleavage appears to be a result of a difference between the s. typhimurium and e. coli rrna sequences rather than to differences in ribonuclease activity. we have surveyed a wide range of enterobacteriaceae for the presence or absence of 23s rrna ... | 1988 | 2455809 |
| structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of shigella flexneri. | the chemical structure of the o-antigen of a proposed new provisional serotype of shigella flexneri has been determined. methylation analysis, glc-ms, 1h-nmr and 13c-nmr showed that the linear o-antigenic polysaccharide is the same as for all s. flexneri [kenne, l., lindberg, b., petersson, k. & romanowska, e. (1977) carbohydr. res. 56, 363-370]. a novel structural feature is that the disaccharide alpha-d-glcp-(1----2)-alpha-d-glcp is linked to o4 of the n-acetyl-glucosamine residue. (formula: s ... | 1988 | 2458260 |
| shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies. | invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ... | 1988 | 2459066 |
| [ultrasonic disintegration of immune complexes for the detection of shigella antigens in the urine of patients with dysentery]. | | 1988 | 2461483 |
| interferon treatment of hep-2 cells alters the adhesive ability of pseudomonas aeruginosa. | previous studies have shown that interferons specifically interact with hep-2 cells and reduce invasiveness by salmonella and shigella. in this study an effect of interferons on the adhesiveness of a strictly extracellular bacterium was demonstrated. hep-2 cells were treated with human leukocyte interferon, recombinant interferon alpha-a or recombinant interferon gamma and the adhesiveness of pseudomonas aeruginosa to the cells was examined using a light microscopy method. a recent isolate and a ... | 1988 | 2461717 |
| [evaluation of the efficacy of elective differential media and their combinations for isolating shigella and salmonella]. | | 1988 | 2462097 |
| bacterial epitopes involved in the induction of reactive arthritis. | a panel of documented arthritogenic shigella flexneri strains as well as an epidemic-associated non-arthritogenic shigella sonnei control was used to identify a 2-megadalton plasmid specific to the arthritogenic strains. the plasmid, phs-2, contains a dna sequence that encodes a 22-amino acid polypeptide encompassing a pentapeptide homologous to part of the polymorphic region of the alpha-1 domain of hla-b27. these results suggest that molecular mimicry between arthritogenic bacterial-encoded ep ... | 1988 | 2462352 |
| [stimulation of the secretory iga system in monkeys by parenteral immunization with a ribosomal sonne dysentery vaccine]. | the experiment was made on 16 monkeys (rhesus macaques). only 1 out of 12 monkeys immunized with s. sonnei ribosomal vaccine and all 4 control monkeys fell ill as the result of oral challenge with s. sonnei virulent strain. the immunized monkeys stopped excreting shigellae earlier than the control monkeys. antibody to lipopolysaccharide (lps) in the serum and saliva of the monkeys were studied in the enzyme immunoassay with monospecific antibodies to human iga, igg and igm. a single injection of ... | 1988 | 2462772 |
| somatic antigens of shigella: structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide. | 4-(n-acetylglycyl)amino-4,6-dideoxy-d-glucose has been identified as a component of the shigella dysenteriae type 7 o-specific polysaccharide, in addition to the previously reported 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galacturonic acid. on the basis of selective cleavage with anhydrous hydrogen fluoride and analysis by 1h- and 13c-n.m.r. spectroscopy and f.a.b.-mass spectrometry, it was concluded that the tetrasaccharide repeating-unit of the polysaccharide has the following ... | 1988 | 2463086 |
| immunoglobulin m, a, and g antibody response to lipopolysaccharide o antigen in symptomatic and asymptomatic shigella infections. | the antilipopolysaccharide antibody response in sera obtained from subjects involved in 10 outbreaks of shigellosis occurring in israeli military field units was determined by an enzyme-linked immunosorbent assay and a passive hemagglutination test. both tests were found to be sensitive and specific for the diagnosis of shigellosis. a significant antibody response was detected in 73 to 82% of the symptomatic and 48 to 60% of the asymptomatic subjects during the shigella sonnei and shigella flexn ... | 1989 | 2463995 |
| [specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants]. | the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ... | 1988 | 2464261 |
| usefulness of the stool wright's stain in the emergency department. | a prospective study was conducted to determine if a wright's stain of stool specimen to detect fecal leukocytes was accurate in predicting the presence of a bacterial pathogen on stool culture. entry criteria were patient age greater than or equal to 3 months and diarrhea of greater than 1 day. the patient population was drawn from an urban county hospital emergency department on the texas-mexican border. a total of 69 patients were evaluated by both routine stool culture and stool wright's stai ... | 1988 | 2464634 |
| purification and characterization of a phage-encoded cytotoxin from an escherichia coli o111 strain associated with hemolytic-uremic syndrome. | cytotoxin production by escherichia coli o111:h-strain hus-2 (hamburg) is associated with a temperate toxin-converting bacteriophage (tcp-111). e. coli laboratory strain c600 transduced and subsequently lysed by the phage produced and liberated large amounts of cytotoxin (ct111) which was purified by sequential chromatography. when compared with published procedures for toxin release from viable cells, lysis of the c600 culture by the phage was most effective. by sds-page ct111 as shiga toxin fr ... | 1988 | 2464885 |
| [immunotropic activity of cyclophosphane]. | the sensitivity of ram erythrocyte-specific b-cells to cyclophosphane was studied. an antigen and two polyclonal activators, lipopolysaccharide and dextran sulfate, were used as the clone activators. the immunodepressant effect of cyclophosphane was found to depend on the degree of the immunocompetent clone activation. the maximal elimination of the clone under the influence of cyclophosphane occurred after its activation by the antigen and lipopolysaccharide. the drug exerted the minimal effect ... | 1988 | 2466690 |
| monoclonal antibodies delineate multiple epitopes on the o antigens of salmonella typhi lipopolysaccharide. | fifteen monoclonal antibodies (mabs) directed against salmonella typhi were produced and characterized. the specificities of the antibodies were determined by their binding patterns in an enzyme immunoassay, with a panel of lipopolysaccharides isolated from different bacteria. seven mabs reacted with s. typhi, salmonella enteritidis, and salmonella dublin (all belonging to serogroup d). one mab also reacted with salmonella paratyphi a and s. paratyphi b. five mabs reacted with s. typhi, s. enter ... | 1988 | 2466868 |
| enteropathogens isolated from children with gastro-enteritis at ga-rankuwa hospital, south africa. | a prospective 3-year study for enteric pathogens associated with diarrhoea in children was undertaken at ga-rankuwa hospital, south africa. rotavirus was the most commonly isolated pathogen (24%), showing a marked seasonal distribution, with an autumn peak in activity. enterotoxigenic and enteropathogenic escherichia coli were frequently identified, and salmonella, shigella and campylobacter were also isolated. cryptosporidium and giardia lamblia were the most frequently seen parasites. rotaviru ... | 1988 | 2467616 |
| [comparative diagnostic value of methods for detecting dysentery antigens in substrates of the patient's body]. | the comparative evaluation of different immunological methods, such as the enzyme immunoassay, the aggregate hemagglutination test and the complement fixation test, used for the detection of specific shigella antigens in biological body substrates obtained from 287 patients with acute dysentery caused by s. sonnei, s. flexneri and s. newcastle has been carried out. the enzyme immunoassay and the aggregate hemagglutination test most effective (97.5 +/- 0.5 and 92.4 +/- 0.9, respectively), the obj ... | 1989 | 2469267 |
| feeding by mucin and intestinal growth of some enteric bacterial pathogens. | enteroinvasive escherichia coli, salmonella typhi-murium, shigella sonnei, shigella flexneri, as well as e. coli k-12 show dose dependent growth in minimal medium completed with purified hog gastric "granular mucin". this ability is based on alpha-galactosidase production: defective, melibiose (and galactose) non-fermenting k-12 mutant were unable to utilize mucin. the viability of the parent k-12 strain in the cecal content of mice is significantly higher than that of its mel- mutant phenotype. ... | 1988 | 2469280 |
| effects of interferon and tumour necrosis factor on development of intracellular bacterial infections. | invasiveness of the epithelial cells in the gut by salmonella, shigella, enteroinvasive escherichia coli and other facultatively intracellular enteropathogenic bacteria, is an important pathogenetic factor in the course of infection. this process is inhibited when cells are pretreated with different types of interferons as demonstrated in animal and in culture models. the tumour necrosis factor seems to have minor effect on this process. mostly the internalization process is reduced, and, to a l ... | 1988 | 2469282 |
| [incompatibility and the copy number of plasmid pkym is regulated by ribonucleic acid]. | | 1988 | 2469791 |
| cloning of the rfb gene region of shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. | recent studies have shown that determinants for the production of o antigen lipopolysaccharide in shigella dysenteriae 1 are distributed over two distinct genetic elements, the chromosome and a 9 kb plasmid designated phw400. in this communication, we describe the cloning of all determinants necessary for s. dysenteriae 1 o antigen production in e. coli k-12 and their combination in a single plasmid. an rp4::minimu r-prime plasmid, r-prime 40, containing the his-rfb (histidine biosynthesis-lipop ... | 1986 | 2469931 |
| genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: 9 kb plasmid of s. dysenteriae 1 determines addition of a galactose residue to the lipopolysaccharide core. | production of the somatic antigen, o-specific polysaccharide of shigella dysenteriae 1 is determined by the chromosomal rfb gene cluster and the rfp gene located on the 9 kb plasmid phw400 carried by this organism. when transferred to escherichia coli k-12, which produces lipopolysaccharide consisting only of core oligosaccharide linked to lipid a, rfp gene-containing plasmids caused modification of the core oligosaccharide leading to the appearance of core molecules with new electrophoretic mob ... | 1986 | 2469932 |